638 문수경ㆍ김인수ㆍ고준철ㆍ박혜진ㆍ정보영 n-3 PUFA (Huynh and Kitts, 2009; Saito et al., 1999). 20 n-3 PUFA. 재료및방법 재료 Ko et al. (2015) (2015 ), 11 (2016 ) (Table

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1 한수지 50(6), , 2017 Original Article Korean J Fish Aquat Sci 50(6), ,2017 제주도산아열대성어류 20 종의지방산조성특징 문수경 김인수 고준철 1 박혜진 2 정보영 * 경상대학교식품영양학과 / 농업생명과학연구원, 1 국립수산과학원제주수산연구소, 2 창신대학교식품영양학과 Fatty Acid Composition of 20 Subtropical Fish Species from Jeju Island Soo-Kyung Moon, In-Soo Kim, Jun-Cheol Ko 1, Hye-Jin Park 2 and Bo-Young Jeong* Department of Food and Nutrition/Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Korea 1 Jeju Fisheries Research Institute, National Institute of Fisheries Science, Jeju 63068, Korea 2 Department of Food Science and Nutrition, Changshin University, Changwon 51352, Korea Lipid levels and fatty acid compositions of 20 species of subtropical fish caught off the coast of Jeju Island, Korea were determined. The fish were categorized according to total lipid (TL) content as follows; lean fish (TL=1.19±0.32%, n=10 species), low-fat fish (TL=3.02±0.44%, n=6 species) and medium-fat fish (TL=6.87±1.23%, n=4 species). The prominent fatty acids were saturated fatty acids (SFAs), at 16:0 and 18:0 monounsaturated fatty acids (MUFAs), at 18:1n-9 and 16:1n-7, and polyunsaturated fatty acids (PUFAs), at 22:6n-3, 20:5n-3 and/or 20:4n-6. Across all species, the percentages of PUFAs and SFAs were 38.0±7.89% and 34.3±3.08%, respectively, with no significant difference between both groups (P<0.05), but showed higher proportions than that of MUFA group (27.8±7.23%) (P<0.05). The proportion of n-3 PUFAs was 27.1±8.68% across all fish species, similar to levels in other fishes from Korea. These results suggest that subtropical fish from Jeju Island are a good source of n-3 PUFAs for humans. Key words: Lipid content, Subtropical fish, n-3 Polyunsaturated fatty acid, Jeju island. 서론 (Jeong et al., 2003; Seong et al., 2010). (Min and Kim, 2006). 41 ( ) 1.17 ( ), (0.67 ) 2 (Suh et al., 2011; Belkin, 2009; Jang et al., 2006).,. Ko et al. (2015) , 49.4%. (MFDS, 2017a),., 20:5n-3 (eicosapentaenoic acid, EPA), 22:6n-3 (docosahexenoic acid, DHA) n-3 (polyunsaturated fatty acid, PUFA) (Ackman, 1989; Jeong et al., 1998b), n-3 PUFA (Kinsella, 1988; Hirayama, 1990; Yazawa and Kageyama, 1991; Breslow, 2006; Johnson and Schaefer, 2006; Hibbeln et al., 2007; Moon et al., 2014), n-3 PUFA (Bradberry and Hilleman, 2013; MFDS, 2017b).,,,,. (, ) (, ) Korean J Fish Aquat Sci 50(6) , December 2017 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Licens ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Received 14 November 2017; Revised 20 November 2017; Accepted 22 November 2017 *Corresponding author: Tel: Fax: address: byjeong@gnu.ac.kr Copyright 2017 The Korean Society of Fisheries and Aquatic Science 637 pissn: , eissn:

2 638 문수경ㆍ김인수ㆍ고준철ㆍ박혜진ㆍ정보영 n-3 PUFA (Huynh and Kitts, 2009; Saito et al., 1999). 20 n-3 PUFA. 재료및방법 재료 Ko et al. (2015) (2015 ), 11 (2016 ) (Table 1). ( ).,,,. speed cutter (NFM-8860, NUC Co. Ltd., Korea), -70 (WUF-500, DAIHAN Scientific Co. Ltd., Korea). 2, 2 4. 총지질및지방산조성분석 (total lipid, TL) Bligh and Dyer (1959). TL AOCS (1998) GC-2010 Plus (Shimadzu, Ltd., Kyoto, Japan)., TL 14% BF 3 -Methanol (AOCS, 1998). TL Omegawax 320 fused silica capillary column (30 m 0.32 mm 0.25 µm film thickness, Supelco, Inc. Bellefonte, USA) Table 1. Biological data of 20 species of subtropical fish caught off the coast of Jeju island Korean name Scientific name Common name Body length Body weight No. of Collection (cm) (g) sample date 1. Yeol-ssang-dong-ga-ri Parapercis multifasciata Bicolor-barred weever 14.9± ± Dec Ga-si-bog Diodon holocanthus Linnaeus Balloonfish 17.8± ± Aug Sae-da-rae Brama japonica Pomfret 33.7± ± Jul Yug-dong-ga-ri-dom Evistias acutirostris Banded boarhead 22.0± ± Jun Gin-ggo-ri-beng-e-dom Girella melanichthys Small scale blackfish 22.4± ± Jul Ho-bag-dom Choerodon azurio Scar breast tuskfish 19.8± ± Aug Geo-bug-bog Ostracion immaculatus Black-spotted boxfish 20.3± ± Aug No-rang-ben-ja-ri Callanthias japonicus Yellowtail red bass 19.7± ± Jul Ssug-gam-peng Scorpaenopsis cirrhosa Hairy stingfish 22.3± ± Aug Cheong-jul-dom Chaetodontoplus septentrionalis Blue-lined angelfish 13.1± ± Aug Dog-ga-si-chi Siganus fuscescens Rabbit fish 25.2± ± Aug A-hob-dong-ga-ri Goniistius zonatus Whitespot-tail morwong 23.9± ± Aug Hwang-nol-rae-gi Pseudolabrus sieboldi Bamboo leaf wrasse 16.5± ± Aug Yeo-deorb-dong-gai-ri Goniistius quadricornis Black barred morwong 25.4± ± Jul Beom-dom Microcanthus strigatus Stripey 11.9± ± Aug Mu-jeom-hwang-nolrae-gi Pseudolabrus eoethinus Red naped wrasse 14.7± ± Aug Gu-gal-dom Lethrinus haematopterus Red collared emperor 27.3± ± Aug Du-jul-chog-su Pseudupeneus spilurus Japanese goatfish 27.8± ± Jun Ben-ja-ri Parapristipoma trilineatum Three line grunt 23.9± ± Jul Ja-ri-dom Chromis notata Coralfish 9.9± ± Jun. 2015

3 제주도산아열대성어류지방산조성 639 gas chromatograph (GC-2010 Plus, Shimadzu Seisakusho, Co, Ltd. Kyoto, Japan). (injector) FI (flame ionization) (detector) 250, (column oven) /min Carrier gas He (54.0 ml/min), split ratio 1:50. (Supelco 37 Component FAME Mix., Sigma-Aldrich Korea, Seoul, Korea) (retention time), (Ackman, 1986; Moon et al., 2005) ECL (equivalent chain length). methyl tricosanoate (99%, Sigma-Aldrich Korea, Seoul, Korea). 통계분석 SPSS 12.0 (SPSS Inc., Chicago, IL, USA) (SD), (one-way analysis of variance) P<0.05 Duncan's multiple range tests. 결과및고찰 총지질함량 Table (total lipid, TL). TL 0.71% 8.23%, TL 4 (Ackman, 1989; Huynh and Kitts, 2009).,,,,,,,,,, 10 TL % lean fish [LF, TL 2%,, (KAST, 1998) ],,,,,, 6 TL % low-fat fish (LFF, TL 2-4%, ),,,, 4 TL % medium-fat fish (MFF, TL 4-8%, ). TL 9% high-fat fish (HFF, ). 20 TL Jeong et al. (1998a, b). TL 8.26% (Jeong et al., 1998a) TL (1.26%)., Table 2. Total lipid (TL) content and fatty acid compositions (% of total fatty acids) of five species of lean fish (LF) in 20 species of subtropical fish caught off the coast of Jeju island* Lean fish (TL<2%) 1. Bicolor-barred weever 2. Balloonfish 3. Pomfret 4. Banded boarhead 5. Small scale blackfish 14: ±0.11 c 0.28 ±0.01 a 0.56 ±0.03 b 2.90 ±0.04 g 2.33 ±0.06 f 15:0 iso ± : ± ± ± ± ± :0 DMA ± ± ± ± ± : ±0.11 e ±0.17 a ±0.07 h ±0.34 c ±0.02 h 17:0 iso 0.50 ± ± ± ± ± : ± ± ± ± ± :0 iso ± ± ± ± :0 DMA 0.76 ± ± ± ± ± : ±0.04 j ±0.02 o 6.17 ±0.02 h 8.27 ±0.39 l 7.11 ±0.04 k 20: ± ± ± ± ± : ± ± : ± ±0.00 Total SFA :1n ± ± :1n ±0.02 a 2.76 ±0.03 c 1.99 ±0.06 b 4.47±0.02 g 2.96 ±0.05 d

4 640 문수경ㆍ김인수ㆍ고준철ㆍ박혜진ㆍ정보영 Table 2. Continued Lean fish (TL < 2%) 1. Bicolor-barred weever 2. Balloonfish 3. Pomfret 4. Banded boarhead 5. Small scale blackfish 16:1n ± ± ± ± :1n ± ± ± ± ± :1 DMA ± ± ± :1n ± ± :1n ±0.03 a 9.60 ±0.02 c ±0.23 h ±0.74 d ±0.11 e 18:1n ±0.01 c 4.20 ±0.00 k 1.79 ±0.01 b 2.12 ±0.11 c 2.15 ±0.00 c 18:1n ± ± ± ± :1n ± ± ± ± :1n ±0.01 cd 2.29 ±0.03 i 0.80 ±0.01 cd 1.63 ±0.62 g 1.04 ±0.01 def 20:1n ± ± ± ± ± :1n ±0.07 ab 0.37 ±0.01 ab 0.21 ±0.01 a 1.67 ±0.88 e 0.43 ±0.02 ab 22:1n ± ± ± :1n ± Total MUFA :2n ± ± ± ± :2n ± ± ± ± ± :2n ± ± ± ± ± :2n ± ± :3n ± ± ± ± ± :3n ± ± ± ± ± :3n ± ± ± ± ± :4n ± ± ± :4n ± :2n ± ± ± ± ± :3n ± ± ± :4n ±0.05 k ±0.10 r 1.91 ±0.01 c 7.49 ±0.22 p 4.32 ±0.03 j 20:3n ± ± ± :4n ± ± ± ± ± :5n ±0.03 k 4.19 ±0.03 e 3.26 ±0.03 c 8.83 ±0.06 p 6.13 ±0.02 l 22:2 NMID ± ± :3n ± ± :4n ±0.02 m 4.87 ±0.07 p 0.16 ±0.01 a 1.07 ±0.04 e 0.77 ±0.04 c 22:5n ± ± ± ± ± :5n ±0.02 n 7.35 ±0.10 q 1.28 ±0.01 b 2.44 ±0.08 f 2.77 ±0.02 g 22:6n ±0.02 n ±0.12 e ±0.38 o ±0.60 g ±0.13 l Total PUFA TL (%) 0.71±0.01 a 0.82 ±0.03 a 0.87±0.01 a 1.09 ±0.01 b 1.25 ±0.01 c n n DMA, dimethyl acetal. 2 NMID, nonmethylene-interrupted diene. SFA, saturated fatty acid; MUFA, monounsaturated fatty acid; PUFA, polyunsaturated fatty acid. *Results represent means ± SD; superscripts denote significant differences (P<0.05) between all fish species (20 species).

5 제주도산아열대성어류지방산조성 641 Table 3. Total lipid content and fatty acid compositions (% of total fatty acids) of five species of lean fish (LF) in 20 species of subtropical fish caught off the coast of Jeju island* Lean fish (TL < 2%) 6. Scar breast tuskfish 7. Black-spotted boxfish 8. Yellowtail red bass 9. Hairy stingfish 10. Blue-lined angelfish 14: ±0.25 l 1.77 ±0.02 d 2.06±0.01 e 3.47 ±0.02 i 1.92 ±0.06 de 15:0 iso ± : ± ± ± ± ± :0 iso 0.18 ± ± ± :0 DMA ± ± ± ± ± : ±0.03 ef ±0.03 g 22.61±0.33 k ±0.05 j ±0.20 ij 17:0 iso 0.55 ± ± ± ± ± : ± ± ± ± ± :0 iso 0.34 ± ± ± ± ± :0 DMA 0.42 ± ± ± ± ± : ±0.06 h ±0.01 n 5.11±0.04 cd 5.97 ±0.04 g 5.69 ±0.08 f 20: ± ± ± ± ± : ± : ± ± ±0.02 Total SFA :1n ± ± ± ± :1n ±0.15 e 5.84 ±0.01 j 5.17±0.37 h 7.00 ±0.01 l 6.92 ±0.12 l 16:1n ± ± ± :1n ± ± ± ± ± :1n ±0.01 f ±0.01 m 11.39±0.02 fg ±0.05 g ±0.00 l 18:1n ±0.05 ef 4.92 ±0.03 l 2.47±0.03 d 2.11 ±0.00 c 4.86 ±0.10 l 18:1n ± ± ± ± ± :1n ± ± ± ± :1n ±0.01 de 0.35 ±0.02 a 3.52±0.01 l 1.78 ±0.00 gh 0.63 ±0.01 bc 20:1n ± ± ± ± ± :1n ±0.02 ab ±0.88 g 1.06 ±0.02 d - 22:1n ± ± :1n ± ± Total MUFA :2n ± ± ± ± :2n ± ± ± ± ± :2n ± :2n ± ± ± ± ± :2n ± ± :3n ± ± ± ± ± :3n ± ± ± ± ± :3n ± ± ± ± ± :4n ± ± ± ± :2 NMID ± :2n ± ± ± ± ± :3n ± ± ± ±0.01

6 642 문수경ㆍ김인수ㆍ고준철ㆍ박혜진ㆍ정보영. 6 (2015 ),,. 7 (1995 ) TL. 20 TL (0.71%- 8.26%, %) Jeong et al. (1998a,b) (TL, %, %) 14 (TL, %, %), 26 (TL, %, %) TL, (Kim et al., 2005) Jeong et al. (1998a,b). (Kim et al., 2005). 지방산조성 20 14:0 22:6n-3 40 (Tables 2, 3, 4, 5). TL 5% 16:0, 18:0 (saturated fatty acid, SFA) 18:1n-9, 16:1n-7 (monounsaturated fatty acid, MUFA), 22:6n-3 (docosahexaenoic acid, DHA), 20:5n-3 (eicosapentaenoic acid, EPA) PUFA. 20 PUFA ( %)>SFA ( %)>MUFA ( %) PUFA SFA MUFA, PUFA SFA (P<0.05) (Table 6). 10 PUFA ( %)>SFA ( %)>MUFA ( %) (P<0.05), SFA ( %)>PUFA ( %)>MUFA ( %), SFA MUFA, SFA PUFA, PUFA MUFA (P<0.05). 4 MUFA ( %)>SFA ( %)>PUFA ( %), (P<0.05). SFA, Table 3. Continued Lean fish (TL<2%) 6. Scar breast tuskfish 7. Black-spotted boxfish 8. Yellowtail red bass 9. Hairy stingfish 10. Blue-lined angelfish 20:4n ±0.08 n 8.45 ±0.01 q 1.26±0.02 b 3.00 ±0.02 f 6.51 ±0.17 o 20:3n ± ± :4n ± ± ± ± ± :5n ±0.02 l 3.45 ±0.00 d 4.55±0.06 f 4.92 ±0.01 h 2.54 ±0.07 a 22:2 NMID 1.74 ± ± ± :3n ± ± :4n ±0.03 h 3.17 ±0.01 o ±0.03 d 2.23 ±0.04 l 22:5n ± ± ± ± ± :5n ±0.02 i 4.87 ±0.03 n 2.26±0.03 e 2.12 ±0.02 d 4.97 ±0.14 o 22:6n ±0.19 h 5.59 ±0.06 a 27.25±0.40 m ±0.08 k 9.15 ±0.17 d Total PUFA TL (%) 1.29±0.14 cd 1.33 ±0.05 cd 1.37±0.01 cd 1.45±0.07 d 1.73 ±0.13 e n n DMA, dimethyl acetal. 2 NMID, nonmethylene-interrupted diene. SFA, saturated fatty acid; MUFA, monounsaturated fatty acid; PUFA, polyunsaturated fatty acid. *Results represent means ± SD; superscripts denote significant differences (P<0.05) between all fish species (20 species).

7 제주도산아열대성어류지방산조성 643 Table 4. Total lipid content and fatty acid compositions (% of total fatty acids) of five species of low-fat fish (LFF) in 20 species of subtropical fish caught off the coast of Jeju island* Low-fat fish (TL, 2-4%) 11. Rabbit fish 12. Whitespot-tail morwong 13. Bamboo leaf wrasse 14. Black barr- ed morwong 15. Stripey 14: ±0.01 hi 2.87 ±0.01 g ±0.23 m 2.88 ±0.01 g 2.39 ±0.01 f 15: ± ± ± ± ± :0 iso ± ± ± :0 DMA ± : ±0.09 m ±0.05 f ±0.13 ef ±0.08 d ±0.09 m 17:0 iso 0.33 ± ± ± ± ± : ± ± ± ± ± :0 iso 0.67 ± ± :0 DMA ± ± ± : ±0.02 b 8.83 ±0.01 m 5.81 ±0.13 f 5.39 ±0.01 e 6.58 ±0.04 i 20: ± ± ± ± ± : ± ± : ± Total SFA :1n ± ± ± ± :1n ± :1n ±0.01 o 5.27 ±0.02 h 4.30 ±0.01 f 5.52 ±0.03 i 4.44 ±0.02 fg 16:1n ± ± :1n ± ± ± ± ± :1n ± ± ± :1n ±0.02 c ±0.04 n ±0.17 j ±0.04 n ±0.10 o 18:1n ±0.01 j 4.06 ±0.01 k 3.22 ±0.06 g 3.28 ±0.01 gi 3.00 ±0.00 fg 18:1n ± ± ± ± ± :1n ± ± ± ± :1n ±0.00 ab 1.06 ±0.01 ef 3.00 ±0.07 k 1.98 ±0.01 h 1.22 ±0.00 f 20:1n ± ± ± ± ± :1n ±0.01 ab 0.63 ±0.01 bc 0.56 ±0.01 abc 1.11 ±0.01 d 0.57 ±0.01 abc 22:1n ± ± ± :1n ±0.01 Total MUFA :2n ± ± ± ± :2n ± ± ± ± ± :2n ± ± ± :2n ± ± ± ± ± :2n ± ± :3n ± ± ± ± ± :3n ± ± ± ± ± :3n ± ± ± ± :4n ± ± ± ± ± :2 NMID ± ± :2n ± ± ± ± ±0.00

8 644 문수경ㆍ김인수ㆍ고준철ㆍ박혜진ㆍ정보영 Table 4. Continued Low-fat fish (TL, 2-4%) 11. Rabbit fish 12. Whitespot-tail morwong 13. Bamboo leaf wrasse 14. Black barr- ed morwong 15. Stripey 20:3n ± ± ± :4n ±0.09 l 4.99 ±0.05 m 4.08 ±0.02 i 2.82 ±0.02 e 4.53 ±0.03 k 20:3n ± :4n ± ± ± ± ± :5n ±0.03 b 7.95 ±0.00 o 6.91 ±0.21 m 9.32 ±0.11 q 4.79 ±0.09 g 22:2 NMID 0.28 ± ± ± ± ± :3n ± ± :4n ±0.01 g 3.01 ±0.01 n 1.54 ±0.04 i 1.13 ±0.01 f 2.18 ±0.00 k 22:5n ± ± ± ± ± :5n ±0.06 p 3.73 ±0.01 k 2.94 ±0.03 h 4.68 ±0.02 m 4.04 ±0.05 l 22:6n ±0.18 i 6.25 ±0.13 b 8.13 ±0.11 c ±0.09 g 6.52 ±0.02 b Total PUFA TL (%) 2.24±0.02 f 2.89±0.11 g 2.90±0.05 g 3.27±0.09 h 3.34±0.17 hi n n DMA, dimethyl acetal. 2 NMID, nonmethylene-interrupted diene. SFA, saturated fatty acid; MUFA, monounsaturated fatty acid; PUFA, polyunsaturated fatty acid. *Results represent means ± SD; superscripts denote significant differences (P<0.05) between all fish species (20 species). PUFA, MUFA (Huynh and Kitts, 2009). guppy Kayama et al. (1963). (, 5 62 N) 5 SFA ( %)>MUFA ( %)>PUFA ( %) (Arai et al., 2015), (, N) 6 SFA ( %)>MUFA ( %)>PUFA ( %) (Metilio and Aspiras-Eye, 2014). SFA PUFA 10%. 10 (, N) SFA ( %)>PUFA ( %)>MUFA ( %) (Dhaneesh et al., 2012), 3 (, 26 15'N) (, N) SFA ( %)>PUFA ( %)>MUFA ( %) (Saito et al., 1999). SFA, PUFA. Jeong et al. (1998b) (, 34 85'N) PUFA ( %, %)>MUFA ( %, %)>SFA ( %, %) (Jeong et al., 1998b), PUFA. 20 (, 33 25'N) PUFA ( %) SFA ( %)>MUFA ( %) MUFA. 8 (Özogul et al., 2007) PUFA>SFA>MUFA. 8 (British Columbia Alaskan) PUFA ( %)>MUFA ( %)>SFA ( %) (Huynh and Kitts, 2009), South Georgia (, 54 S) 10 PUFA ( %)>MUFA ( %)>SFA ( %) (Stowasser et al., 2012).,,,, PUFA, MUFA.

9 제주도산아열대성어류지방산조성 645 Table 5. Total lipid content and fatty acid compositions (% of total fatty acids) of one species of LFF and four species of medium-fat fish in 20 species of subtropical fish caught off the coast of Jeju island* LFF Medium-fat fish (TL, 4-8%) 16. Red naped wrasse 17. Red collared emperor 18. Japanese goatfish 19. Three line grunt 20. Coralfish 14: ±0.05 m 3.27 ±0.07 hi 3.15±0.03 h 4.13 ±0.06 j 5.79±0.42 k 15:0 iso ± :0 anteiso ± : ± ± ± ± ± :0 iso ± : ±0.03 cd ±0.18 hi 23.76±0.08 l ±0.10 j 16.86±0.56 b 17:0 iso 0.38 ± ± ± ± ± : ± ± ± ± ± :0 iso 0.25 ± ± ± ± ± :0 DMA ± ± : ±0.02 f 6.63 ±0.02 i 5.20±0.01 d 4.96 ±0.01 c 3.34±0.03 a 20: ± ± ± ± ± : ± ± ± ±0.07 Total SFA :1n ± ± ± ± :1n ±0.01 e 6.90 ±0.12 l 7.96±0.00 n 6.28 ±0.03 k 7.58±0.12 m 16:1n ± ± ± ± :1n ± ± ± ± ± :1 DMA ± :1n ± :1n ±0.02 i ±0.00 p 15.87±0.01 l ±0.02 k 7.49±0.13 b 18:1n ±0.01 c 3.13 ±0.01 gh 3.06±0.33 i 2.74 ±0.00 e 1.50±0.01 a 18:1n ± ± ± ± :1n ± ± ± ± :1n ±0.01 l 1.26 ±0.01 f 1.59±0.01 g 2.54 ±0.01 j 10.83±0.10 m 20:1n ± ± ± ± ± :1n ±0.01 f 0.61 ±0.02 abc 0.79±0.01 cd 2.57 ±0.02 g 14.29±0.17 h 22:1n ± ± ± ± ± :1n ± Total MUFA :2n ± ± ± ± ± :2n ± ± ± ± ± :2n ± ± ± :2n ± ± ± ± ± :2n ± ± ± :3n ± ± ± ± ± :3n ± ± ± ± :3n ± ± ± ± ± :4n ± ± ± ± ± :4n ± :2 NMID ± ± ±0.42 -

10 646 문수경ㆍ김인수ㆍ고준철ㆍ박혜진ㆍ정보영 Table 5. Continued LFF Medium-fat fish (TL, 4-8%) 16. Red naped wrasse 17. Red collared emperor 18. Japanese goatfish 19. Three line grunt 20. Coralfish 20:2n ± ± ± ± ± :3n ± :4n ± ± ± ± ± :3n ± ± ± :4n ± ± ± ± ± :5n ±0.02 i 4.85 ±0.02 gh 5.68±0.06 j 4.93 ±0.02 h 7.19 ±0.13 n 22:2 NMID 0.42 ± ± ± :3n ± ± ± ± :4n ±0.03 f 1.85 ±0.04 j 1.04±0.02 de 0.33 ±0.00 b - 22:5n ± ± ± ± ± :5n ±0.01 c 3.79 ±0.03 k 2.34±0.09 e 3.25 ±0.04 j 0.61 ±0.01 a 22:6n ±0.11 h ±0.20 e 16.08±0.15 i ±0.26 j ±0.38 f Total PUFA TL (%) 3.45±0.01 i 5.42 ±0.04 j 6.41±0.05 k 7.38±0.26 l 8.26±0.14 m n n DMA, dimethyl acetal. 2 NMID, nonmethylene-interrupted diene. SFA, saturated fatty acid; MUFA, monounsaturated fatty acid; PUFA, polyunsaturated fatty acid. *Results represent means ± SD; superscripts denote significant differences (P<0.05) between all fish species (20 species). (homeoviscous adaptation). PUFA (Farkas,1979; Gladyshev et al., 2011). PUFA n-3 PUFA DHA EPA, n-6 PUFA 20:4n-6. DHA 20.4% ( 14.0%) ( 10.9%). (40.0%), (34.5%), (27.3%), (26.5%), (22.2%) 5 DHA 20%, 20 (P<0.05). TL PUFA PUFA ( ) PUFA triglyceride ( ) (Fogerty et al., 1986). EPA 4.98%, ( 6.21%) ( 5.63%). 20:4n %, ( 4.07%) ( 1.75%). 20:4n-6 (14.1%), (8.45%), (7.49%), (6.51%), (6.30%) (P<0.05). 20:4n-6. (Kim et al., 2005). Jeong et al., (1998b) 72 (8.85%), (7.45%), (5.62%) 20:4n-6, (NIFS, 1994; Chyung, 1991). (Siganus canaliculatus) 20:4n-6 (Saito et al., 1999).. 20:4n-6 EPA 22:5n-3, DHA (Jeong et al., 1998c), (Jeong et al., 1993; Dawczynski et al., 2007).

11 제주도산아열대성어류지방산조성 647 Table 6. The SFA, MUFA and PUFA compositions (% of total fatty acids) in all fish (20 species), lean fish (10 species), low-fat fish (6 species) and medium-fat fish (4 species)* Category SFA MUFA PUFA All fish 34.3±3.23 b 27.8±7.01 a 37.9±7.71 b Lean fish 33.7±2.55 b 23.3±5.55 a 43.1±7.20 c Low-fat fish 36.3±3.90 b 29.9±3.51 a 33.8±4.41 ab Medium-fat fish 32.7±2.92 a 35.9±5.80 a 31.4±3.16 a SFA, saturated fatty acid; MUFA, monounsaturated fatty acid; PUFA, polyunsaturated fatty acid. *Results represent means±sd; superscripts denote significant differences (P<0.05) between all fish species (20 species). SFA PUFA 14:0. SFA 14:0 6.23% ( 2.40%) ( 4.13%). SFA 16:0 ( 21.1%) ( 20.2%), ( 21.0%). MUFA, 20:1n-9 22:1n :1n %, 1.36% 1.87%. 22:1n % 0.75% 0.88%. 20:1n-9 22:1n % 14.3%, 20 (P<0.05). 20:1n-9 22:1n-11 (15.5% 14.36%) (5.20% 6.37%) (Huynh and Kitts, 2009), (12.4% 15.3%)(Budge et al., 2002) (16.3% 24.4%)(Copeman and Parrish, 2004). 2 MUFA copepod ( ) (Graeve et al., 1994; Budge et al., 2002). wax esters 20:1 22:1 fatty alcohol 20:1n-9 22:1n-11 (Tocher, 2003). (Kim et al., 2005), 99.3% (Ko and Jeon, 1983). 20:1n-9 22:1n-11. (Jeong et al., 1998b) 20:1n- 9 22:1n-11., (Kayama et al., 1963; Jeong et al., 1998b,c). n-3 PUFA,,, (Kayama et al., 1963). 20 DHA, EPA n-3 PUFA % ( %) 14 ( %) (Jeong et al., 1998b). n-3 PUFA (Metilio and Aspiras-Eye, 2014) n-3 PUFA ( %, 13.2%) 14%. (Huynh and Kitts, 2009) n-3 PUFA ( %, 38.2%) 10%. (Huynh and Kitts, 2009; Saito et al., 1999)., 20 TL, (Jeong et al., 1998a,b) TL n-3 PUFA. 사사 References Ackman RG WCOT (capillary) gas-liquid chromatography. In: Analysis of oils and fats. Hamilton RJ and Rossell JB, eds. Elsevier Applied Science Publishers Ltd., London and New York, U.K. and U.S.A., Ackman RG Nutritional composition of fats in seafoods. Prog Food Nutr Sci 13, AOCS (American Iol Chemists' Society) AOCS official method Ce 1b-89. In: Official Methods and Recommended Practice of the AOCS (5th Ed). Firestone D, ed. AOCS,

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