9 박천만ㆍ김기혁ㆍ문혜나ㆍ여인규 UV, (Mtsung et l., ; Li et l., ; Mrtinez-Huiltle nd Enri, ).. (Stoner et l., 9; Rhmn et l., ). mixed oxidnt (MO). 재료및방법 실험균 rin hert

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1 한수지 (), 9-9, Originl Artile Koren J Fish Aqut Si (),9-9, 제주도양식넙치 (Prlihthys oliveus) 에서분리한병원균 종에대한 Mixed Oxidnt 및차아염소산나트륨살균효과 박천만 김기혁 문혜나 여인규 * 제주대학교해양생명과학과 Effet of Mixed Oxidnts nd Sodium Hypohlorite on Pthogeni Miroorgnisms in Olive flounder Prlihthys oliveus Aquulture on Jeju Islnd Cheonmn Prk, Ki-hyuk Kim, Hye-n Moon nd In-Kyu Yeo* Deprtment of Mrine Life Siene, Jeju Ntionl University, Jeju, Kore Mrine pthogeni teri, suh s Streptoous prueris, Edwrdsiell trd nd Virio hrveyi, n use lethl infetions in frmed fish, ozone nd ntiiotis, re employed to sterilize wters used for rering fish to mitigte this thret. The most widely used method is tretment with sodium hypohlorite solution. However, the mintenne of onstnt onentrtion of hlorine in rering wters n e diffiult. We investigted the potentil of mixed oxidnt (MO) solution generted y eletrolysis of se wter to improve wter qulity. We mesured the survivl rtes of fish pthogeni teri exposed to different onentrtions of MO (.,.,. nd. MO) nd sodium hypohlorite (.,.,. nd. ppm) for vrious lengths of time (,,,,, nd min). We found time-dependent derese in the survivl rtes of the tested pthogeni miroorgnisms. The steriliztion effet of the MO solution on pthogeni orgnisms ws greter thn tht of sodium hypohlorite for grm-negtive nd grm-positive teri. We onlude tht MO solution produed y eletrolysis ould e used to mintin onstnt hlorine onentrtion in quulture systems. Key words: Eletrolysis, Mixed oxidnt, Sodium hypohlorite, Steriliztion effet, Pthogeni teri 서론,,,9., (MOMAF, 99; NFRDI, ; Sttistis Kore, )., (Kim et l., ).,, (Letux nd Meyer, 9; Rphel et l., 9; Fox et l., 9; Spere et l., 99).,, (Brton nd Iwm, 99; Pikering, 99).,,. Koren J Fish Aqut Si () 9-9, August This is n Open Aess rtile distriuted under the terms of the Cretive Commons Attriution Non-Commeril Liens ( whih permits unrestrited non-ommeril use, distriution, nd reprodution in ny medium, provided the originl work is properly ited. Reeived April ; Revised My ; Aepted 9 June *Corresponding uthor: Tel: +... Fx: E-mil ddress: ikyeo99@jejunu..kr Copyright The Koren Soiety of Fisheries nd Aquti Siene 9 pissn:-, eissn:-

2 9 박천만ㆍ김기혁ㆍ문혜나ㆍ여인규 UV, (Mtsung et l., ; Li et l., ; Mrtinez-Huiltle nd Enri, ).. (Stoner et l., 9; Rhmn et l., ). mixed oxidnt (MO). 재료및방법 실험균 rin hert infusion (BHI, Difo, USA), slmonell-shigell (SS, Difo, USA) thiosulfte itrte ile slts surose (TCBS, Difo, USA),, ml,., rpm.. genomi DNA Prep Kit (SolGent, Kore) DNA. S rrna F; -AGAGTTTGATCMTG- GCTCAG-, 9R; -GGTTACCTTGTTACGACTT- Polymerse hin retion (PCR) (Lne, 99; Ludwing, ). PCR 9, 9,, yle, PCR (US/MYgein, MJ RESERCH, USA). S rdna SolGent. NCBI (ntionl enter for iotehnology informtion) BLAST (si lol lignment serh tool) serh progrm Gennk dtse Ez Txon-e ( dtse. dtse lustl W softwre multiple lignment. MO 및차아염소산나트륨희석액제조 sodium hyphlorite solution.-.99% (Sigm ldrih, USA).,.,.. ppm. MO ( LF),, MO.,.,.. MO. MO 및차아염소산나트륨농도별어류병원세균살균능및유효농도측정 MO (,,,,, ) gr plting. phosphte uffer sline (PBS) BHI gr L olony ml. MO hlorine poket photometer (Hh in., USA).,. MO 의적정중화반응평가 MO.% NCl PBS, % sodium thiosulfte. hlorine poket photometer (Hh in., USA). 통계처리 MO SPSS version (SPSS In., USA) One-wy ANOVA-test. Dunn s multiple test, P<..

3 병원균에대한 MO 및차아염소산나트륨살균효과 9 V. hrveyi (log CFU/ml) V. hrveyi (log CFU/ml) Treted time (min). ppm. ppm. ppm. ppm E.trd (log CFU/ml) E. trd (log CFU/ml) Treted time (min). ppm. ppm. ppm. ppm Treted time (min) Treted time (min) Fig.. Growth urves of Virio hrveyi with different exposure times of mixed oxidnt (MO) nd sodium hypohlorite. Eh point represents the men of optil density determintions. Different supersript letters (,, ) indite signifint differene etween the vlues (P<.). Error rs re omitted. 결과 실험균의동정 Virio sp. V. hrveyi 99%, Edwrdsiell sp. E. trd % Streptoous sp. S. prueris % (dt not shown). MO. 병원균에대한살균효과 MO. Log CFU/mL (Fig. -). V. hrveyi. ppm,. ppm. ppm MO. MO,. MO Fig.. Growth urves of Edwrdsiell trd with different exposure times of mixed oxidnt (MO) nd sodium hypohlorite. Eh point represents the men of optil density determintions. Different supersript letters (,, ) indite signifint differene etween the vlues (P<.). Error rs re omitted.. MO (Fig. ). E. trd. ppm,. ppm. ppm, MO. MO,. MO. MO (Fig. ). S. prueris. ppm,. ppm. ppm MO. MO,. MO. MO (Fig. ). 병원균살균시 MO 및차아염소산나트륨의경시적농도변화 MO., MO,, MO S. prueris (Log CFU/ml)

4 9 박천만ㆍ김기혁ㆍ문혜나ㆍ여인규 S. prueris (Log CFU/ml) S. prueris (log CFU/ml) Treted time (min) Fig.. Growth urves of Streptoous prueris with different exposure times of mixed oxidnt (MO) nd sodium hypohlorite. Eh point represents the men of optil density determintions. Different supersript letters (,, ) indite signifint differene etween the vlues (P<.). Error rs re omitted. (Fig., ). V. hrveyi MO (Fig. )., (Dt not shown). MO. MO 의적정중화반응평가. ppm. ppm. ppm. ppm Treted time (min) MO Tle..% NCl MO, PBS. % sodium thiosulfte MO. Residul Chlorine (ppm) Residul Chlorine (ppm) (C) Residul Chlorine (ppm) Fig.. Effetive hlorine onentrtion of Virio hrveyi, Edwrdsiell trd nd Streptoous prueris (C) with different exposure times of mixed oxidnt (MO). Eh point represents the men of optil density determintions. Error rs re omitted. Tle. Redution effet of mixed oxidnt (MO) ording to ddition of different neutrlizing gent,.% NCl, % sodium thiosulfte nd phosphte uffered sline (PBS) Neutrlizing gent Treted time (min).% NCl % Sodium thiosulfte PBS

5 병원균에대한 MO 및차아염소산나트륨살균효과 9 Sodium thiosulfte, Sodium thiosulfte V. hrveyi, E. trd S. prueris (dt not shown). 고찰 9, (Nguyen nd Kni, 999; Lee et l., ; Kng et l., ; Kim et l., ). S. prueris S. prueris,, (Bek et l., ; Kng et l., ). (Hwng et l., )., E. trd, V. hrveyi (Alvrez et l., 99; Ann nd Rimstd, )., (Mtsungg et l., 99; Nkyme et l., 99; Mtsungg et l., ).., MO (Kerwik et l., ). MO MO (Fig. -). MO (NCl) (Cl - ), (H ) (H ) (OH - ) (OH - ) (N + ) (NOH). (Cl ) (NOH) MO (Ptermrkis nd Fountoukidis, Residul Chlorine (ppm) Residul Chlorine (ppm) (C) Residul Chlorine (ppm) ppm. ppm. ppm. ppm. ppm. ppm. ppm. ppm. ppm. ppm. ppm. ppm Fig.. Effetive hlorine onentrtion of Virio hrveyi, Edwrdsiell trd nd Streptoous prueris (C) with different exposure times of sodium hypohlorite. Eh point represents the men of optil density determintions. Error rs re omitted. 99). MO (Boyln et l., ; Tk nd Prk, 9; Joshi et l., ), Ltoous grviee V. lginolytius, E. trd V. nruillrum MO (Tnk et l., ). V. hrveyi E. trd S. prueris MO

6 9 박천만ㆍ김기혁ㆍ문혜나ㆍ여인규 (Fig. -)..,, Clostridium otulinum, Cmpyloter jejun (Smiert, 9; Merrell et l., 9; Buk et l., 9; Blser et l., 9). (Lee et l., ; Dio et l., ), (Prk et l., ). MO (Fig.. ). MO MO (Rhmn et l., )., (Onhorhynhus mykiss), (Mugil ephlus) (Miropterus slmoides) (Brungs, 9; Dress et l., ; Kerwik et l., ; Fng et l., ). (Ptinopeten yessoensis) UV (Jorquer et l., )..% NCl BHI (Prk et l., ), % sodium thiosulfte Cl + H O + N S O HCl + H SO + N SO MO., MO.% NCl % sodium thiosulfte., MO, MO, V. hrveyi E. trd S. prueris. MO. 사사. Referenes Alvrez JJ, Austin B, Alvrze AM nd Reyes H. 99. Virio hrveyi: A pthogen of peneid shirimps nd fish in Venezuel. J Fish Dis, -. Ann k nd Rimstd E.. Intivtion of infetious slmon virus, virl hemorrhgi septiemin virus nd infetious pnreti nerosis virus in wter using UVC irrdition. Dis Aqu Org, -. Bek GW, Kim JH, Gomez DK nd Prk SC.. Isoltion nd hrteriztion of Streptoous sp. from disesed flounder (Prlihthys oliveus) in Jeju islnd. J Vet Si, -. Brton BA nd Iwm GK. 99. Physiologil hnges in fish from stress in quulture with emphsis on the response nd effets of ortiosteroids. Annu Rev Fish Dis, -. Blser MJ, Smith PF, Wng WLL nd Hoff JC. 9. Intivition of mpyloter jejuni y hlorine nd monohlormine. Appl Environ Miroiol, -. Boyln JA, Lwrene KA, Downey JS nd Gherrdini FC.. Borreli urgdorferi memrnes re the primry trgets of retive oxygen speies. Mol Miroiol, Brungs WA. 9. Effets of residul hlorine on quti life. J Wter Pollut Control Fed, -9. Buk GE, Prshll KA nd Dvis CP. 9. Eletron mirosopy of the ooid form of Cmpyloter jejuni. J Clin Miroiol, -. Dio HF, Li XY, Gu JD, Shi HC nd Xie ZM.. Eletron mirosopi investigtion of the teriidl tion of eletrohemil disinfetion in omprison with hlorintion, ozontion nd fenton retion. Proess Biohem 9, -. Drees KP, Aszdegn M nd Mier RM,. Comprtive eletrohemil intivtion of teri nd teriophge. Wter Res, ()9-. Fng Q, Shng C nd Chen G.. MS intivtion y

7 병원균에대한 MO 및차아염소산나트륨살균효과 9 hloride-ssisted eletrohemil disinfetion. J Environ Eng, ():(). Fox CH, Johnson FB, Whiting J nd Roller PP. 9. Formldehyde fixtion. J Histohem Cytohem, -. Hwng SD, Woo SH, Kim SH, H SJ, Jung YE nd Prk SI.. Immunomodultory hrteristis of Streptoous prueris isolted from infeted olive flounder, Prlihthys oliveus. J Fish Pthol, -. Jorquer MA., Vleni G, Eguhi M, Ktyose M nd Riquelme C.. Disinfetion of sewter for hthery quulture systems using eletrolyti wter tretment. Aquulture, -. Joshi SG, Cooper M, Yost A, Pff M, Ern UK, Fridmn G, Friedmn G, Fridmn A nd Brooks AD.. Nontherml dieletri-rrier dishrge plsm-indued in tivtion involves oxidtive DNA dmge nd memrne lipid peroxidtion in Esherihi oli. Antimiro Agents nd Chemother, -. Kni K. 99. Bteril diseses of flounder, Prlihthys oliveus. J Fish Pthol, 9-. Kng CY, Kng BJ, Moon YG, Kim KY nd Heo MS.. Chrteriztion of Streptoous prueris isolted from ultured olive flounder, Prlihthys oliveus in the Jeju Islnd. J Fish Pthol 9, -. Kerwik MI, Reddy SM, Chmerlin AHL nd Holt DM.. Eletrohemil disinfetion, n environmentlly eptle method of drinking wter disinfetion?. Eletrohim At, -. Kim JW, Cho MY, Prk GH, Won KM, Choi HS, Kim MS nd Prk M.. Sttistil dt on infetious diseses of ultured olive flounder Prlihthys oliveus from to. J fish pthol, 9-. Kim JW, Lee HN, Jee BY, Woo SH, Kim YJ nd Lee MK.. Monitoring of the mortlities in the quulture frms of south kore. J Fish Pthol, -. org/./jfp... Lne DJ. 99. S/S rrna sequening. In: Nulei id tehniques in teril systemtis. Stkerndt, E nd Goodfellow M, Eds.. Lee CH, Kim PY, Ko CS, Oh DC nd Kng BJ.. Biologil hrteristis of Streptoous inie nd Streptoous prueris isolted from ultured flounder, Prlihthys oliveus in Jeju. J Fish Pthol, -. Lee SJ, Lee DC, Kim H nd Lee H.. Comprison of hlorine, hlorine dioxide nd ozone s disinfetnts in drinking wter. Kor J Env Hlth So, -. Letux F nd Meyer FP. 9. Mixture of mlhite green nd formline for ontroling Ihthyophthirius nd other protozon prsites of fish. Prog Fish Cult org/./-(9)[:momgf]..o;. Li XY, Ding F, Lo PSY nd Sin SHP.. Eletrohemil disinfetion of sline wstewter effluent. J Environ Eng, ():(9). Ludwig W.. Nulei id tehniques in teril systemtis nd identifition. Int j food miroiol, -. Mrtinez-Huitle CA nd Enri B.. Eletrohemil lterntives for drinking wter disinfetion. Angew Chem Int Ed., Mtsung T, Nksono S, Kitjim Y nd Horiguhi K. 99. Eletrohemil disinfetion of teri in drinking wter using tivted ron fiers. Biotehnol Bioeng, Mtsung T, Okohi M, Tkhshi M, Nkym T, Wke H nd Nkmur N.. TiN eletrode retor for disinfetion of drinking wter. Wter Res, -. org/./s-()-x. Merrell BR, Wlker BI nd Coolugh JC. 9. Cmpyloter fetus ss. Jejuni, newly reognized enteri pthogen: morphology nd intestinl oloniztion. Snning Eletron Mirose, -. MOMAF (Ministry of Mritime Affirs nd Fisheries). 99. Sttistil yerook of mritime ffirs nd fisheries. Ministry of Mritime Affirs nd Fisheries, Seoul, Kore. Nkym T, Wke H, Ozw K, Kodm H, Nkmur N nd Mtsung T. 99. Use of titnium nitride for eletrohemil intivtion of mrine teri. Environ Si Tehnol, NFRDI (Ntionl Fisheries Reserh nd Development Institute).. Prevention of teril fish disese nd medil tretment for produe helth fish. Ntionl Fisheries Reserh nd Development Institute, Busn, Kore. Nguyen HT nd Kni K Seletive grs for the isoltion of Streptoous inie from Jpnese flounder, Prlihthys oliveus nd its ulturl environment. J Appl Miroiol, 9-. ttps://doi.org/./j x. Plumo SA. 9. Het injury nd repir in Cmpyloter jejuni. Appl Envirion Miroiol, -. Prk KH, Oh MJ nd Kim HY.. Disinfetion effet of hlorine dioxide on pthogeni teri. J quulture, -. Ptermrkis G nd Fountoukidis E. 99. Disinfetion of wter y eletrohemil tretment. Wter Res, Pikering AD. 99. Rinow trout husndry: mngement of the stress response. Aquulture, org/./-(9)9-n. Rphel D, Wong TA, Moodnik R nd Borden BG. 9. The effet of temperture on the teriidl effiieny of sodium hypohlorite. J Endod, -.

8 9 박천만ㆍ김기혁ㆍ문혜나ㆍ여인규 s99-99()-x. RhmnSME Ding T nd Oh DH.. Effetiveness of low onentrtion eletrolyzed wter to intive foodorne pthogens under different environmentl onditions. Int J Food Miroiol 9, -. Smiert RM. 9. The genus Cmpyloter. Ann Rev Miroiol, Spere DJ, Goff G, MIs P, Wehrkiwsky J nd MNir N. 99. Effets of formlin nd hlormine-t tretments on oxygen onsumption of juvenile slmonids. J Aquti Anim Helth (99)<:eoft>..o;. Sttistis Kore.. Aquulture Sttus Survey. Sttistis Kore, Dejeon, Kore. Stoner GE, Chen GLJ, Shyni M nd Giledi E. 9. The mehnism of low frequeny.. eletrohemil disinfetion. Bioeletrohem Bioenerg 9, org/./-9()-. Tk JK nd Prk JW. 9. The use of eselen for rdioprotetion in ultured ells nd mie. Free Rdi Biol Med, -. Tnk T, Shimod M, Hosokw M, Tguhi T Wke H nd Mtsung T.. Eletrohemil disinfetion of fish pthogens in sewter without the prodution of lethl onentrtion of hlorine using flow retor. J iosi ioeng, -. jios...

Kor J Fish Aquat Sci 44(6), DOI : 44(6), , 2011 순치기간에따른육봉형산천어 (Oncorhynchus masou masou) 의해수적

Kor J Fish Aquat Sci 44(6), DOI : 44(6), , 2011 순치기간에따른육봉형산천어 (Oncorhynchus masou masou) 의해수적 Kor J Fish Aqut Sci 44(6), 753-758 DOI : http://dx.doi.org/.5657/kfas.211.753, 44(6), 753-758, 211 순치기간에따른육봉형산천어 (Oncorhynchus msou msou) 의해수적응능력 김병기ㆍ김재원 ㆍ박정환 1 ㆍ성기백 2 ㆍ김현주 3 강원도립대학해양생명과학과, 1 노스캐롤라이나주립대학생물학과,