KMB ` 실험실탐방 국민대학교식품생물공학연구실 (Food and BioTechnology Lab., FBTL) 연구책임자 : 박용철교수소속기관 : 국민대학교 Department of Bio and Fermentation Convergence Technology, Kookmin University 77 Jeongneung-Ro, Seongbuk-Gu, Seoul 136-702, South Korea Tel: 02)910-5596, Fax: 02)910-5739 E-mail: ycpark@kookmin.ac.kr Homepage: http://fbtl.kookmin.ac.kr 책임교수소개 [ 학력 ] 1994. 02. 서울대학교식품공학과농학사 1996. 02. 서울대학교협동과정생물화학공학전공공학석사 2003. 08. 서울대학교협동과정생물화학공학전공공학박사 [ 경력 ] 2014. 01.~ 현재 Associate Editor of Journal of Microbiology and Biotechnology 2014. 03.~ 현재국민대학교바이오GMP기술인력양성사업바이오화학과정책임자 2009. 09.~ 현재국민대학교바이오발효융합학과조교수, 부교수 2006. 07.~2009. 08. 서울대학교농업생물신소재연구소연구교수 2004. 07.~2006. 06. 박사후연구원, Department of Biochemistry and Cell Biology, Rice University, U.S.A. 1
2003. 09.~2004. 07. 서울대학교 BK21 농생명공학사업단박사후연구원 1997. 09.~1999. 08. 동양제과주식회사기술개발연구소연구원 1996. 07.~1997. 09. 진로종합연구소연구원 실험실구성원 박용철 ( 연구책임자 ), 김수정 ( 박사후연구원 ), 이혜진 ( 연구원 ), 성영제 ( 박사과정 ), 박미경 ( 석사과정 ), 박해성 ( 석사과정 ), 조용한 ( 석사과정 ), 이현수 ( 석사과정 ), 김보미 ( 석사과정 ), 전영리 ( 학부생 ), 김지영 ( 학부생 ) 2
` 연구분야 소개 (1) 주요 연구분야 대사공학(metabolic engineering)을 이용한 식품 및 산업소재의 대량생산기술 개발 (bioethanol, 2,3-butanediol, 3-hydroxypropionic acid, isobutylene, D-lactate, D-ribose, xylitol 등) 난발효성 탄수화물을 이용한 고부가가치 소재 개발 (알긴산, 펙틴, 한천 등) 생물전환기술(biotransformation)을 이용한 엔지니어링 플라스틱 원료(dicarboxylic acid) 생산기술개발 유전자 조작에서부터 목적산물(G-TOP, Gene-To-Product)의 발효생산까지 시스템과 발효공정의 글로벌 최적화 기술(GO SysPro, Global Optimization of Systems and Process) 개발 3
(2) 연구주제 1) 시스템생물공학기반식품유래고성능효모플랫폼개발 미래형바이오공정에필요한미생물시스템은 (1) 높은온도 압력 산도, 고농도알코올 유기산 염류에대한다중내성을보유하고 (2) 유전자조작이가능하고 (3) 알코올 유기산등의고기능성바이오케미컬생산에이용가능한특성을보유해야함. 본연구과제의최종목표는미래형바이오공정에적합한식품유래고성능유전자재조합다중내성효모플랫폼을개발하는것임 4
2) 장쇄디카르복실산생산공정개발 탄소수 9~13개로구성된장쇄디카르복실산은폴리아미드 / 나일론및폴리에스터, 폴리우레탄합성의단량체또는화학 / 화장품 / 식품소재로광범위하게이용되고있는중요한화학소재임. 예를들어나이론-610, hot melt adhesives, 부식방지제, plasticizers, greases, 수지, 유화제, hydraulic fluids, 양초제조시사용되고또한 aromatics, antiseptics, painting materials 생산시중간체로사용되고있는 C10 디카르복실산인세바식산의수요는연평균 15-20% 로증가하는것으로예상됨 현재까지장쇄디카르복실산을상업적으로생산하는방법은지방산을원료로화학촉매공정및오존의사용으로환경문제를야기시키고있어서청정기술인생물공정을이용한기술개발을위해선진국을중심으로대규모투자를하고있음 곧, 본연구과제의최종목표는효율적인장쇄디카르복실산생산용생물공정및분리정제공정을개발하는것임 3) 섬유소바이오매스공정부산물의고부가가치응용제품용중간원료화및공통기술개발 비식용그린카본은농림부산물, 초 / 목본류등을포함하는섬유소바이오매스 (cellulosic biomass) 로정의하고, 섬유소바이오매스는셀룰로오스 (cellulose) 와헤미셀룰로오스 (hemicellulose) 의탄수화물중합체와리그닌 (ligin) 의방향족화합물중합체로이루어져있음 비식용그린카본을바이오슈가로전환시키기위해다양한전처리 (pretreatment) 방법이개발되어왔고묽은산, 암모니아, 초임계수와 organosolv 등의촉매를주로사용함 모든전처리공정은주요산물로셀룰로오스를, 공정부산물로헤미셀룰로오스와리그닌을분리하여회수함 셀룰로오스는당화공정을통해서포도당으로전환되어바이오공정의원료 (feedstock) 로이용되는반면, 헤미셀룰로오스당류와리그닌의활용기술개발은현재까지미흡함 5
곧, 본과제의최종목표는바이오슈가공정부산물인헤미셀룰로오스당류와리그닌을이용하여고부 가가치응용제품의중간체를제조할수있는플랫폼생촉매 (platform biocatalyst) 를개발하는것임. 4) 자일로스이송체가강화된고초균을이용한식품소재라이보스생산메커니즘규명 라이보스는고부가가치식품용소재로리보플라빈과향미증진제의원료로사용됨. 라이보스생산용균주는대부분고초균인바실러스서브틸리스로서대사효소트랜스케톨래이즈를보유하고있지않고섬유소유래의자일로스를비특이적으로흡수함. 곧, 섬유소유래의자일로스를효과적으로라이보스생산에이용하기위해서자일로스특이이송체가도입된라이보스생산균주를개발해야함. 본연구의최종목표는자일로스이송체유전자가강화된바실러스서브틸리스균주를제조하고자일로스를원료로이용한라이보스의생산메커니즘을규명하여식품소재라이보스를효율적으로발효생산할수있는최적의시스템을구축하는것임 자일로스유도형벡터시스템개발및자일로스이송체유전자의라이브러리구축 자일로스이송체발현시스템과재조합바실러스서브틸리스개발및자일로스이송메커니즘규명 자일로스이송체강화형바실러스서브틸리스를이용한라이보스생산기술개발 5) 알길산분해효소대량생산기술개발 해조류바이오매스의일종이갈조류는알긴산, 섬유소, 라미나란등의탄수화물로이루어져있음 이중알긴산은전체갈조류의 60% 이상을차지하기때문에갈조류를이용한바이오소재의생산을위해서는알긴산을분해하여단량체로전환시키는기술개발이필수적임 본연구에서는해양미생물에서유래한알긴산분해효소를유전자재조합기술을이용하여대량생산하고생산된효소를이용한갈조류알긴산단량체를생산하기위한효소공정을개발함 6
6) 갈조류탄수화물기반바이오에탄올생산균주및발효공정개발 갈조류의주요탄수화물인알긴산을이용하여바이오에탄올을생산할수있는유전자재조합미생물을개발함 알긴산단량체는난발효성단당류로이를이용하려면유전공학, 미생물공학및대사공학등의기술을이용하여단량체를출발물질로한대사경로를재설계해야함. 곧, 대사재설계된유전자재조합미생물을이용하여난발효성알긴산단량체를원료로바이오케미컬류를생산할수있는 platform 기술을개발하고자함 대표연구성과 (selected from 2012) (1) 논문 Kwang-wook Kim, Sun-Ki Kim, Yong-Cheol Park*, and Jin-Ho Seo (2014) Enhanced production of 3-hydroxypropionic acid from glycerol by modulation of glycerol metabolism in recombinant Escherichia coli. Bioresource Technology, 156, 170-175. So-Yeon Shin, Yi-Hyun Bae, Sun-Ki Kim, Yeong-Je Seong, Suk-Hwan Choi, Kyoung Heon Kim, Yong-Cheol Park*, and Jin-Ho Seo (2014) Effects of signal sequences and folding accessory proteins on extracellular expression of carboxypeptidase Y in recombinant Saccharomyces cerevisiae. Bioprocess and Biosystems Engineering, 37(6): 1065-1071 Soo-Jung Kim, Seung-Oh Seo, Yong-Cheol Park, Yong-Su Jin, Jin-Ho Seo (2014) Production of 2,3-Butanediol from xylose by engineered Saccharomyces cerevisiae. Journal of Biotechnology Yi-Hyun Bae, Dae-Hyuk Kweon, Yong-Cheol Park, Jin-Ho Seo (2014) Deletion of the HXK2 gene in Saccharomyces cerevisiae enables mixed sugar fermentation of glucose and galactose in oxygen-limited conditions. Process Biochemistry, 49(4):547-553. Sung-Hyuk Han, Jung-Eun Lee, Kyung-moon Park and Yong-Cheol Park* (2013) Production of 2,3-butanediol by a low-acid producing Klebsiella oxytoca NBRF4. New Biotechnology, 30(2): 166-172. Su-ryang Kwak, Yong-Cheol Park* and Jin-Ho Seo (2013) Biosynthesis of 3-hydroxypropionic acid from glycerol in recombinant Escherichia coli expressing Lactobacillus brevis dhab and dhar gene clusters and E. coli K-12 aldh. Bioresource Technology, 135, 4323-439 7
Yong-Cheol Park, Hye-Jin Lee, Chang-Sup Kim, Jin-Ho Seo (2013) Effects of oxygen supply and mixed sugar concentration on D-ribose production by a transketolase-deficient Bacillus subtilis SPK1. Journal of Microbiology and Biotechnology, 23(4): 555-559. Soo-Rin Kim, Yong-Cheol Park, Yong-Su Jin and Jin-Ho Seo (2013) Strain engineering of Saccharomyces cerevisiae for enhanced xylose metabolism. Biotechnology Advances, 31(6): 851-861 Young-Wook Chin, Jin-Byung Park, Yong-Cheol Park, Kyung Heon Kim and Jin-Ho Seo* (2013) Metabolic engineering of Corynebacterium glutamicum to produce GDP-L-fucose from glucose and mannose. Bioprocess and Biosystems Engineering, 36(6):749-756. Yong-Cheol Park, Soo-Young Jun and Jin-Ho Seo (2012) Construction and characterization of recombinant Bacillus subtilis JY123 able to transport xylose efficiently. Journal of Biotechnology, 161(4): 402-406. Sung-Haeng Lee, Tsutomu Kodaki, Yong-Cheol Park* and Jin-Ho Seo (2012) Effects of NADH-preferring xylose reductase expression on ethanol production from xylose in xylose-metabolizing recombinant Saccharomyces cerevisiae. Journal of Biotechnology, 158(4): 184-191. Young-Kyung Park, Sang-Min Jung, Hyung-Kwon Lim, Young-Jin Son, Yong-Cheol Park* and Jin-Ho Seo (2012) Effects of Trx2p and Sec23p expression on stable production of hepatitis B surface antigen S domain in recombinant Saccharomyces cerevisiae. Journal of Biotechnology, 160(3-4): 151-160. Nam-Soo Han, Tae-Jip Kim, Yong-Cheol Park, Jae-han Kim and Jin-Ho Seo (2012) Biotechnological production of human milk oligosaccharides. Biotechnology Advances, 30(6): 1268-1278. Young-Hoon Jung, Soo-ah Kim, Taek-Ho Yang, Hee-Jong Lee, Do-young Seung, Yong- Cheol Park, Jin-Ho Seo, In-Geol Choi, Kyoung Heon Kim (2012) Aqueous ammonia pretreatment, saccharification, and fermentation evaluation of oil palm fronds for ethanol production. Bioprocess and Biosystems Engineering, 35(9): 1497-1503. Jin-Woo Kim, Young-Wook Jin, Yong-Cheol Park* and Jin-Ho Seo (2012) Effects of deletion of glycerol-3-phosphate dehydrogenase and glutamate dehydrogenase genes on glycerol and ethanol metabolism in recombinant Saccharomyces cerevisiae. 8
Bioprocess and Biosystems Engineering, 35(1-2): 49-54. Eun-Jung Oh, Yi-hyun Bae, Kyoung-Heon Kim, Yong-Cheol Park* and Jin-Ho Seo (2012) Effect of overexpression of acetaldehyde dehydrogenase 6 and acetyl-coa synthetase 1 on xylitol production in recombinant Saccharomyces cerevisiae. Biocatalysis and Agricultural Biotechnology, 1, 15-19. Seung-Phill Choi, Yong-Cheol park, Jung-Hwa Lee, Sang-Jun Sim, and Ho-Nam Chang (2012) Effects of L-arginine on refolding of lysine-tagged human insulin-like growth factor 1 expressed in Escherichia coli. Bioprocess and Biosystems Engineering, 35(1-2): 255-263. Young-Wook Chin, Jin-Woo Kim, Yong-Cheol Park and Jin-Ho Seo (2012) Comparison of ethanol fermentation properties between laboratorial and industrial yeast strains using cassava hydrolysate. Korean Journal of Microbiology and Biotechnology, 40(3): 220-225. (2) 특허 Jin-Ho Seo, Yong-Cheol Park. Method for producing ethanol from xylose. 미국특허등 록 (patent No. 8628944 B2, issued date Jan. 1, 2014) 서진호, 오선영, 조정현, 박용철. 자일리톨의생산에 NADPH 및 NADH 를공히이용할 수있는재조합효모를이용한자일리톨의생산방법. 대한민국특허출원 10-2013-0109355 ( 출 원일 2013.09.11) Jin-Ho Seo, Yong-Cheol Park. METHOD FOR PRODUCING ETHANOL FROM XYLOSE USING RECOMBINANT SACCHAROMYCES TRANSFORMED TO ELEMINATE FUNCTIONS OF GENES INVOLVED IN TOR SIGNAL TRANSDUCTION PATHWAY. 미국특허출원 (patent No. 2012/0122172 A1, publication date May 17, 2012) Jae Young Kim, Hyun Min Koo, Young Kyoung Park, Jae Chan Park, Sung Min Park, Jin Ho Seo, Yong Cheol Park. MODIFIED MICROORGANISM HAVING ENHANCED XYLOSE UTILIZATION. 미국특허출원 (publication NO. 2012/0329104 A1, publication date Dec. 27, 9
2012) 김재영, 박재찬, 박성민, 구현민, 박영경, 서진호, 박용철. 향상된자일로스이용능력을 갖는변형미생물. 대한민국특허출원 10-2013-0007687 ( 공개일 2013.01.21) 서진호, 박용철, 김광욱, 이종원, 정인영. 재조합대장균을이용하여 3- 히드록시프로피 온산을고수율로생산하는방법. 대한민국특허출원 10-2013-0007108 ( 출원일 2013.01.22) 서진호, 박용철. 토르 (Tor) 신호전달경로에관여하는유전자의기능을소실시킨재조 합사카로마이세스세레비지애를이용하여자일로오스로부터에탄올을생산하는방법. 대한민 국특허등록 10-1204366 호 (2012.11.19) 서진호, 김선기, 박용철. 음이온으로하전된극성사이드체인을갖는아미노산또는 비전하극성사이드체인을갖는아미노산이태그형태로리파아제의 N- 말단에결합하고있는 재조합리파아제및대장균을이용한이의생산방법. 대한민국특허등록 10-1147838 호 (2012.05.14) 김재영, 박재찬, 박성민, 구현민, 박영경, 서진호, 박용철. 향상된자일로스이용능력을 갖는변형미생물. 대한민국특허출원 ( 공개번호 10-2013-0007687, 공개일 2013.01.21) 10