Benchtop Flow Cytometry guava easycyte Systems 머크밀리포아의 guava easycyte System 은특허받은 microcapillary 기술을이용한컴팩트한시스템으로다양하고복잡한세포분석이가능합니다. Cell Health Cell Signaling Stem Cells Immunology Cell Counting & Viability Cell Cycle DNA Damage Mitochondrial Analysis Apoptosis MARK Pathway EGFR Pathway Pl3/Akt/mTOR Pathway Jak/ATAT Pathway Chemokine Embryotic Stem Cell (Human/Mouse) Neural Stem Cell (Rodent) Regulation T-Cell Phenotype Markers
Flow Cytometry Solutions Microcapillary Flow Cytometry Technology guava system은 microcapillary technology를사용하여정확한세포분석이가능합니다. 또한 microcapillary flow cell은 sheath fluid가요구되지않기때문에시료와시약이소량사용되며폐기물이거의발생하지않습니다. 사용자는 microcapillary flow cell을직접교체할수있기때문에언제든지세척과유지관리가용이합니다. Guava-patented microcapillary system Traditional sheath fluid system Sheath Fluid: None : <80mL per 8 hour run Typical Cells Per Protocol: 10 3-10 6 cells/test Microcapillary Sheath Fluid: ~10 ml/test : 8,000 ml per 8 hour run Typical Cells Per Protocol: 10 5-10 6 cells/test Traditional Flow Flow Flow Cell Sheath Fluid Sheath Flow Injection Tip Anatomy of the guava easycyte Systems guava easycyte system은하나 (blue) 또는두개 (blue & red) 레이저가장착되어있으며사용자의연구목적과분석에따라 system을선택할수있습니다. 하나 (blue) 의레이저가장착된모델에는 5/5HT, 6/6HT system이있으며두개 (blue & red) 의레이저가장착된모델에는 6-2L/6HT-2L, 8/8HT system이있습니다. guava easycyte 8/8HT system의경우두개 (blue & red) 레이저를사용하여동시에최대 8개의 parameters(6개의 PMTs, forward scatter, side scatter) 를측정할수있습니다. Excitation laser : blue(488 nm), red(640 nm) Emission : 6 PMTs(photomultipliers) Green, Yellow, Red1, NIR1, Red2, NIR2 Fluorochromes optimized on the eastcyte systems Inside the guava easycyte 8 and 8HT Systems BLUE LASER (488 nm) BLUE LASER (488 nm) GREEN GREEN FITC FITC YELLOW YELLOW PE PE RED1 RED1 PE-Cy5 PE-Cy5 * * PE-Cy5.5 PE-Cy5.5 ** ** NIR1 NIR1 PE- PE- 785 nm 785 nm 661 nm RED LASER (640 nm) RED LASER (640 nm) NIR - Near InfraRed PE - Phycoerythrin FITC - Fluorescein APC - Allophycocyanin RED2 RED2 APC APC NIR2 NIR2 APC- APC- Forward Scatter Side Scatter 583 nm 690 nm 525 nm RED LASER (640 nm) BLUE LASER (488 nm) * Optimized dye for easycyte 5, 6, 5HT and 6HT ** Optimized dye for easycyte 6-2L, 8, 6HT-2L and 8HT Multiwell Plate
guava easycyte Single Microcapillary flow cell 유량보충액 (sheath fluid) 이필요없으며필요시사용자가직접교체가능 Wash vial Flow cell 의고압력세척기능 Up to six-color detection 하나 (blue) 또는두개 (blue, red) 의레이저원을사용하여 6가지의컬러검출 vial 8 시간반응시간동안 80ml 이하로 waste 수집 Small footprint 실험실공간을적게사용가로 : 45.1cm 세로 : 44.5cm 높이 : 22.2cm Robotic sample tray Single tube 로한개씩샘플분석가능 Specifications for single tube format System easycyte 5 easycyte 6 easycyte 6-2L easycyte 8 Catalogue 0500-5005 0500-5006 0500-5007 0500-5008 Type Single tube Single tube Single tube Single tube Blue (488nm) Blue (488nm) FSC SSC Green 525/30nm 525/30nm 525/30nm 525/30nm Yellow 583/26nm 583/26nm 583/26nm 583/26nm Red1 680/30nm 680/30nm 690/50nm 690/50nm NIR1 N/A 785/70nm N/A 785/70nm Red2 N/A N/A 661/19nm 661/19nm NIR2 N/A N/A N/A 785/70nm Microcapillary Fluidics Direct, Absolute cell counts Mixing InCyte Software guavasuite Software modules Digital Signal Processing
guava easycyte High Throughput Sampling Microcapillary flow cell 유량보충액 (sheath fluid) 이필요없으며필요시사용자가직접교체가능 Wash vial Flow cell 의고압력세척기능 Up to six-color detection 하나 (blue) 또는두개 (blue, red) 의레이저원을사용하여 6가지의컬러검출 Small footprint 실험실공간을적게사용가로 : 51.5cm 세로 : 59.0cm 높이 : 25.4cm vial 8 시간반응시간동안 80ml 이하로 waste 수집 Robotic sample tray 96 well plate 를 10 개 sample tube 를사용하여자동분석가능 Specifications for 96 well format System easycyte 5HT easycyte 6HT easycyte 6HT-2HL easycyte 8HT Catalogue 0500-4005 0500-4006 0500-4007 0500-4008 Type 96 well 96 well 96 well 96 well Blue (488nm) Blue (488nm) FSC SSC Green 525/30nm 525/30nm 525/30nm 525/30nm Yellow 583/26nm 583/26nm 583/26nm 583/26nm Red1 680/30nm 680/30nm 690/50nm 690/50nm NIR1 N/A 785/70nm N/A 785/70nm Red2 N/A N/A 661/19nm 661/19nm NIR2 N/A N/A N/A 785/70nm Microcapillary Fluidics Direct, Absolute cell counts Automation-96 well and 10 tubes Mixing InCyte Software guavasuite Software modules Digital Signal Processing
Software guava 소프트웨어는 flexible assay 전용모듈인 InCyte 소프트웨어와 specific assay 전용모듈인 guavasuite 소프트웨어로구성되어있으며, 각각의전용소프트웨어모듈에서시스템설치, 데이터확보및분석이가능합니다. 또한시스템을편리하게 QC할수있는 easycheck과자동으로세척할수있는 guava clean 기능이포함되어있습니다. InCyte Software InCyte 소프트웨어의인터페이스는사용자가정의한파라미터의 heat-map 을표시함으로써다차원의세포분석이가능하며 11 개의데이터를동시에비교 분석할수있습니다. InCyte 소프트웨어는 sirna screens, apoptosis/cell cycle compound screening, high-throughput cell-based assays의결과를해석하는데유용합니다. 마우스로쉽게 gating 하여 drag & drop( 끌어넣기 ) 로다른 plot 으로이동 IC 곡선은초기용량에대한반응곡선범위를보여줌 실시간으로 plot 측정하면서동시에최대 11 plot 을확인 측정한데이타표시및분석시데이타선택란 분석 template 생성 이전분석데이터확인 Heat map 은 single 또는 96-well format 의실험으로부터얻은측정값을파란색의명도차이로표현하여한번에최대 6 개의분석파라미터를확인 Heat map 에동시에비교하고자하는데이터를지정할때사용 (cut-off 값과 threshold 값을조정할때슬라이더바이용 ) Heat map view Candidate Compound Staurosporine 96 well plate 전체데이터를파악하기용이 동시에최대 4개실험데이터를나열가능 파란색의명도차이로측정값의 intensity 표현 Single tube 측정데이터도 heat map 사용가능 Camptothecin 짧은시간안에실험데이터전체적인윤곽파악가능 CASP 8 Ann V CASP 9 MitoP
Analysis Kits Apoptosis Kits 4500-0450 Guava Nexin Reagent 100 Tests 4500-0455 Guava Nexin Reagent 500 Tests 4000-0061 Guava Express 7-AAD Reagent 200 Tests 4500-0121 Guava TUNEL Kit 100 Tests 4500-0121-1 Guava TUNEL Kit Bag 1 of 2, (-20C) 100 Tests 4500-0121-2 Guava TUNEL Kit Bag 2 of 2, (2-8C) 100 Tests 4500-0500 Guava MultiCaspase SR Kit 4500-0510 Guava Caspase 3/7 SR Kit 4500-0520 Guava Caspase 9 SR Kit 4500-0530 Guava MultiCaspase FAM Kit 4500-0540 Guava Caspase 3/7 FAM Kit 4500-0550 Guava Caspase 8 FAM Kit 4500-0560 Guava Caspase 9 FAM Kit 4500-0570 Guava MultiCaspase SR and Caspase 3/7 FAM Kit 4500-0580 Guava MultiCaspase SR and Caspase 8 FAM Kit 4500-0590 Guava MultiCaspase SR and Caspase 9 FAM Kit 4500-0600 Guava Caspase 3/7 SR and Caspase 8 FAM Kit 4500-0610 Guava Caspase 3/7 SR and Caspase 9 FAM Kit 4500-0620 Guava Caspase 3/7 SR and MultiCaspase FAM Kit 4500-0630 Guava Caspase 9 SR and Caspase 3/7 FAM Kit 4500-0640 Guava Caspase 9 SR and Caspase 8 FAM Kit 4500-0650 Guava Caspase 9 SR and MultiCaspase FAM Kit FCCH100105 FlowCellect MitoPotential Red Kit FCCH100106 FlowCellect MitoDamage Kit FCCH100107 FlowCellect MitoLive Kit FCCH100108 FlowCellect Annexin Red Kit FCCH100109 FlowCellect MitoStress Kit FCCH100109-1 FlowCellect MitoStress Kit, Pkg 1 FCCH100109-2 FlowCellect MitoStress Kit, Pkg 2 FCCH100110 FlowCellect Cytochrome c Kit FCCH100110-1 FlowCellect Cytochrome c Kit, Pkg 1 FCCH100110-2 FlowCellect Cytochrome c Kit, Pkg 2 FCCH100110-3 FlowCellect Cytochrome c Kit, Pkg 3 Human Immuno Cell Kit FCCH100137 FlowCellect Human B-cell FAS Kit FCCH100138 FlowCellect Human T-cell Apoptosis Kit FCCH100139 FlowCellect Human T-cell MitoDamage Kit FCCH100140 FlowCellect Human CD8 T-cell FAS Kit FCCH100141 FlowCellect Human T-cell Activation Kit FCCH100154 FlowCellect Human CD4 T-cell FAS Kit FCCH100155 FlowCellect Human T Cell Caspase 8 Kit FCCH100156 FlowCellect Human T Cell Caspase 9 Kit FCCH100157 FlowCellect Human T Cell Caspase 3/7 Kit CD Kit 4500-0150 Guava Express CD3/CD4 Kit 100 Tests 4500-0190 Guava EasyCD8 Reagent Kit 100 Tests 4500-0195RU0 Guava EasyCD8 Reagent Kit 500 Tests 4500-0196RU0 Guava EasyCD8 Reagent Kit 1000 Tests 4500-0480 Guava Auto CD4/CD4% Reagent Kit 100 Tests 4500-0480RU0 Guava Auto CD4/CD4% Reagent Kit 100 Tests 4500-0485 Guava Auto CD4/CD4% Reagent Kit 500 Tests 4500-0485RU0 Guava Auto CD4/CD4% Reagent Kit 500 Tests 4500-0490 Guava Auto CD4/CD4% Reagent Kit 1000Tests 4500-0490RU0 Guava Auto CD4/CD4% Reagent Kit 1000 Tests FlowCellect Chemokine Kits FCXR100420 FlowCellect Chemokine Receptor CXCR1 Surface FCXR200421 FlowCellect Chemokine Receptor CXCR2 Surface FCXR300422 FlowCellect Chemokine Receptor CXCR3 Surface FCXR400423 FlowCellect Chemokine Receptor CXCR4 Surface FCXR600424 FlowCellect Chemokine Receptor CXCR6 Surface Cell Toxicity Kit 4500-0230 Guava Cell Toxicity Kit (EasyCyte) 4500-0230-1 Guava Cell Toxicity Kit (EasyCyte) Bag 1 of 2 (-20C) 4500-0230-2 Guava Cell Toxicity Kit (EasyCyte) Bag 2 of 2 (2-8C) Cell Cycle Kit 4000-0040 Guava ViaCount Reagent 100 Tests 4000-0041 Guava ViaCount Reagent 600 Tests 4500-0110 Guava ViaCount Flex Reagent for Challenging 100 Tests 4500-0220 Guava Cell Cycle Reagent 100 Tests FCCH025102 FlowCellect Bivariate Cell Cycle Kit for DNA Replication FCCH025103 FlowCellect Bivariate Cell Cycle Kit for G2/M Analysis 4500-0270 Guava Cell Growth Kit 200 Tests 4500-0270-1 Guava Cell Growth Kit Bag 1 of 2 (-20C) 200 Tests 4500-0270-2 Guava Cell Growth Kit Bag 2 of 2 (2-8C) 200 Tests Human IgG Kit 4500-0340 Guava RapidQuant Human IgG Kit 4500-0340-1 Guava RapidQuant Human IgG Kit Part 1 4500-0340-2 Guava RapidQuant Human IgG Kit Part 2 4500-0350 Guava Rapid Quant Mouse IgG Kit 4500-0350-1 Guava RapidQuant Mouse IgG Kit Part 1 4500-0350-2 Guava RapidQuant Mouse IgG Kit Part 2 데모문의지금, 영화과학으로신청하세요! 문의전화 : 02-2140-5408 www.merck-millipore.com www.millipore.com/flowcytometry (135-891) 서울특별시강남구신사동 577-7 구정빌딩 Tel : 02-2140-5400 Fax : 02-2140-5405 E-mail : ywsc@youngwha.com Homepage : www.labplus.co.kr 제품상담 : 생명과학사업부 02-2140-5408