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Transcription:

최종연구보고서

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Acetic acid Treatment Non- primed AA 0.001% AA 0.01% AA 0.05% 4 Sprout length(mm) 3 2 1 0 0 12 24 36 48 60 Time(hours) Water-Soluble Chitosan Treatment Non- primed WSC 0.001% WSC 0.01% WSC 0.05% 4 Sprout length(mm) 3 2 1 0 0 12 24 36 48 60 Time(hours) - 52 -

Water-Unsoluble Chitosan Treatment Non- primed WUC 0.001% WUC 0.01% WUC 0.05% 4 Sprout length(mm) 3 2 1 0 0 12 24 36 48 60 Time(hours) Ultra-Sound Treatment (28kHz) 4 Non-primed US 1min US 5min US 10min US 20min US 30min Sprout length(mm) 3 2 1 0 0 12 24 36 48 60 Time(hours) - 53 -

Ultra-Sound Strength 40% Treatment (40kHz) Non-primed US 1min US 5min US 10min US 20min US 30min Sprout length(mm) 4 3 2 1 0 0 12 24 36 48 60 Time(hours) Ultra-Sound Strength 70% Treatment (40kHz) Non-primed US 1min US 5min US 10min US 20min US 30min 4 Sprout length(mm) 3 2 1 0 0 12 24 36 48 60 Time(hours) - 54 -

Ultra-Sound Strength 100% Treatment (40kHz) Non-primed US 1min US 5min US 10min US 20min US 30min 4 Sprout length(mm) 3 2 1 0 0 12 24 36 48 60 Time(hours) Ultra-Sound weakness Treatment (60kHz) Non-primed US 1min US 5min US 10min US 20min US 30min 4 Sprout length(mm) 3 2 1 0 0 12 24 36 48 60 Time(hours) - 55 -

Ultra-Sound Strength strong(100%) Treatment (60kHz) Non primed US 1min US 5min US 10min US 20min US 30min 4 Sproutt length(mm). 3 2 1 0 0 12 24 36 48 60 Time(hours) Germination rate(%) 100 90 80 70 60 50 40 30 20 10 0 Non-primed AA 0.001 AA 0.01 AA 0.05 WSC 0.001 WSC 0.01 WSC 0.05 WUC 0.001 WUC 0.01 WUC 0.05 60hr 48hr 36hr 24hr 12hr Treatment - 56 -

Germination rate(%) 100 90 80 70 60 50 40 30 20 10 0 Non-primed 28 khz 1 min 28 khz 5 min 28 khz 10 min 28 khz 20 min 28 khz 30 min 40 khz 1 min 40 khz 5 min 40 khz 10 min 40 khz 20 min 40 khz 30 min 60 khz 1 min 60 khz 5 min 60 khz 10 min 60 khz 20 min 60 khz 30 min Treatment 60hr 48hr 36hr 24hr 12hr 4.0 Sprout length (mm) 3.0 2.0 1.0 60hr 48hr 36hr 24hr 12hr 0.0 Non-primed AA 0.001 AA 0.01 AA 0.05 WSC 0.001 WSC 0.01 WSC 0.05 WUC 0.001 WUC 0.01 WUC 0.05 Treatment - 57 -

4.0 Sprout length (mm) 3.0 2.0 1.0 60hr 48hr 36hr 24hr 12hr 0.0 Non-primed 28 khz 1 min 28 khz 5 min 28 khz 10 min 28 khz 20 min 28 khz 30 min 40 khz 1 min 40 khz 5 min 40 khz 10 min 40 khz 20 min 40 khz 30 min 60 khz 1 min 60 khz 5 min 60 khz 10 min 60 khz 20 min 60 khz 30 min Treatment - 58 -

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Acetic acid Treatment Non primed AA 0.001% AA 0.01% AA 0.05% 4 Sprout length(mm) 3 2 1 0 0 12 24 36 48 60 Time(hours) Water-Soluble Chitosan Treatment Non primed WSC 0.001% WSC 0.01% WSC 0.05% 4 Sprout length(mm) 3 2 1 0 0 12 24 36 48 60 Time(hours) - 61 -

Water-Unsoluble Chitosan Treatment Non primed WUC 0.001% WUC 0.01% WUC 0.05% Sprout length(mm) 4 3 2 1 0 0 12 24 36 48 60 Time(hours) Ultra-Sound Treatment (28kHz) Non primed US 1min US 5min US 10min US 20min US 30min Sprout length(mm) 4 3 2 1 0 0 12 24 36 48 60 Time(hours) - 62 -

Ultra-Sound Treatment (40kHz) Non primed US 1min US 5min US 10min US 20min US 30min 4 Sprout length(mm) 3 2 1 0 0 12 24 36 48 60 Time(hours) Ultra-Sound Treatment (60kHz) Non primed US 1min US 5min US 10min US 20min US 30min 4 Sprout length(mm) 3 2 1 0 0 12 24 36 48 60 Time(hours) - 63 -

Germination rate(%) 100 90 80 70 60 50 40 30 20 10 0 Non-primed AA 0.001 AA 0.01 AA 0.05 WSC 0.001 WSC 0.01 WSC 0.05 WUC 0.001 WUC 0.01 WUC 0.05 60hr 48hr 36hr 24hr 12hr Treatment Germination rate(%) 100 90 80 70 60 50 40 30 20 10 0 Non-primed 28 khz 1 min 28 khz 5 min 28 khz 10 min 28 khz 20 min 28 khz 30 min 40 khz 1 min 40 khz 5 min 40 khz 10 min 40 khz 20 min 40 khz 30 min 60 khz 1 min 60 khz 5 min 60 khz 10 min 60 khz 20 min 60 khz 30 min Treatment 60hr 48hr 36hr 24hr 12hr - 64 -

4.0 Sprout length (mm) 3.0 2.0 1.0 60hr 48hr 36hr 24hr 12hr 0.0 Non-primed AA 0.001 AA 0.01 AA 0.05 WSC 0.001 WSC 0.01 WSC 0.05 WUC 0.001 WUC 0.01 WUC 0.05 Treatment 4.0 Sprout length (mm) 3.0 2.0 1.0 60hr 48hr 36hr 24hr 12hr 0.0 Non-primed 28 khz 1 min 28 khz 5 min 28 khz 10 min 28 khz 20 min 28 khz 30 min 40 khz 1 min 40 khz 5 min 40 khz 10 min 40 khz 20 min 40 khz 30 min 60 khz 1 min 60 khz 5 min 60 khz 10 min 60 khz 20 min Treatment - 65 -

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Acetic acid Treatment Non-primed AA 0.001% AA 0.01% AA 0.05% 4 Sprout length(mm) 3 2 1 0 0 12 24 36 48 60 Time(hours) - 68 -

Water-Soluble Chitosan Treatment Non-primed W SC 0.001% WSC 0.01% WSC 0.05% 4 Sprout length(mm) 3 2 1 0 0 12 24 36 48 60 Time(hours) Water-Unsoluble Chitosan Treatment Non-primed WUC 0.001% WUC 0.01% WUC 0.05% 4 Sprout length(mm) 3 2 1 0 0 12 24 36 48 60 Time(hours) - 69 -

Ultra-Sound Treatment (28kHz) Non-primed US 1min US 5min US 10min US 20min US 30min 4 Sprout length(mm) 3 2 1 0 0 12 24 36 48 60 Time(hours) Ultra-Sound Treatment (40kHz ) 4 Non-primed US 1min US 5min US 10min US 20min US 30min Sprout length(mm) 3 2 1 0 0 12 24 36 48 60 Time(hours) - 70 -

Ultra-Sound Treatment (60kHz) Non-primed US 1min US 5min US 10min US 20min US 30min 4 Sprout length(mm) 3 2 1 0 0 12 24 36 48 60 Time(hours) Germination rate(%) 100 90 80 70 60 50 40 30 20 10 0 Non-primed AA 0.001 AA 0.01 AA 0.05 WSC 0.001 WSC 0.01 WSC 0.05 WUC 0.001 WUC 0.01 WUC 0.05 60hr 48hr 36hr 24hr 12hr Treatment - 71 -

Germination rate(%) 100 90 80 70 60 50 40 30 20 10 0 Non-primed 28 khz 1 min 28 khz 5 min 28 khz 10 min 28 khz 20 min 28 khz 30 min 40 khz 1 min 40 khz 5 min 40 khz 10 min 40 khz 20 min 40 khz 30 min 60 khz 1 min 60 khz 5 min 60 khz 10 min 60 khz 20 min 60 khz 30 min. Treatment 60hr 48hr 36hr 24hr 12hr 4.0 Sprout length (mm) 3.0 2.0 1.0 60hr 48hr 36hr 24hr 12hr 0.0 Non-primed AA 0.001 AA 0.01 AA 0.05 WSC 0.001 WSC 0.01 WSC 0.05 WUC 0.001 WUC 0.01 WUC 0.05 Treatment - 72 -

4.0 Sprout length (mm) 3.0 2.0 1.0 60hr 48hr 36hr 24hr 12hr 0.0 Non-primed 28 khz 1 min 28 khz 5 min 28 khz 10 min 28 khz 20 min 28 khz 30 min 40 khz 1 min 40 khz 5 min 40 khz 10 min 40 khz 20 min 40 khz 30 min 60 khz 1 min 60 khz 5 min 60 khz 10 min 60 khz 20 min 60 khz 30 min Treatment - 73 -

- 74 -

- 75 -

- 76 -

- 77 -

- 78 -

- 79 -

- 80 -

- 81 -

- 82 -

- 83 -

- 84 -

90 Germination rate (%) 80 70 60 50 40 30 Cotrol(500~900/cc) A1(5,000~9,000/cc) A2(10,000~20,000/cc) A3(400,000~600,000/cc) 24h 36h 48h 60h 72h Time (hours) - 85 -

90 Germination rate (%) 80 70 60 50 40 30 Cotrol(500~900/cc) A4(600,000~900,000/cc) A5(1,000,000/cc) 24h 36h 48h 60h 72h Time (hours) - 86 -

Sprout length (mm) 3.0 2.5 2.0 1.5 1.0 0.5 Cotrol(500~900/cc) A1(5,000~9,000/cc) A2(10,000~20,000/cc) 0.0 24h 36h 48h 60h 72h Time (hours) Sprout length (mm) 3.0 2.5 2.0 1.5 1.0 0.5 Cotrol(500~900/cc) A3(400,000~600,000/cc) A4(600,000~900,000/cc) A5(1,000,000/cc) 0.0 24h 36h 48h 60h 72h Time (hours) - 87 -

- 88 -

- 89 -

- 90 -

- 91 -

- 92 -

- 93 -

- 94 -

- 95 -

- 96 -

- 97 -

- 98 -

- 99 -

100 90 80 Control E-Stimuli Germination rate(% 70 60 50 40 30 20 10 0 24h 36h 48h 60h 72h time (hour) 3.50 3.00 Control E-Stimuli 2.50 Sprout length(mm 2.00 1.50 1.00 0.50 0.00 24h 36h 48h 60h 72h time (hour) - 100 -

- 101 -

- 102 -

- 103 -

- 104 -

- 105 -

- 106 -

- 107 -

- 108 -

3.0 water 2.5min 5min 7.5min 10min 2.5 Sprout length, mm 2.0 1.5 1.0 0.5 0.0 0 10 20 30 40 50 Time, hours - 109 -

- 110 -

- 111 -

- 112 -

- 113 -

- 114 -

- 115 -

- 116 -

- 117 -

연구팀침지발아실험장치 - 118 -

- 119 -

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- 129 -

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- 131 -

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- 133 -

- 134 -

- 135 -

100 90 80 Control Biosystem Germination rate(% 70 60 50 40 30 20 10 0 24h 36h 48h time (hour) - 136 -

Germination rate(%) 100 90 80 70 60 50 40 30 20 10 Control Biosystem 0 24h 36h 48h time (hour) 90 80 70 Control Biosystem Germination rate(%) 60 50 40 30 20 10 0 24h 36h 48h time (hour) - 137 -

3.00 Control 2.50 Biosystem Sprout length(mm 2.00 1.50 1.00 0.50 0.00 24h 36h 48h time (hour) - 138 -

3.00 Control 2.50 Biosystem Sprout length(mm 2.00 1.50 1.00 0.50 0.00 24h 36h 48h time (hour) 3.00 Control 2.50 Biosystem Sprout length(mm 2.00 1.50 1.00 0.50 0.00 24h 36h 48h time (hour) - 139 -

- 140 -

- 141 -

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- 145 -

- 146 -

- 147 -

- 148 -

- 149 -

- 150 -

- 151 -

0 0±0 BA 0.258±0.124 C BC 0.422±0.201 B BC 0.822±0.506 BAC BC 1.729±1.188 BA 0.001 0±0 D 0.064±0.134 BC C 0.366±0.434 BA BC 0.837±0.7567 A C 1.275±1.470 BA 0.01 0±0 DC 0.095±0.136 BC BC 0.432±0.234 BA C 0.672±0.367 BDC C 1.194±0.918 BC 0.05 0±0 0.001 0±0 D 0.051±0.128 C C 0.143±0.160 B D 0.276±0.1584 D D 0.447±0.281 DC A 0.295±0.227 A A 0.758±0.505 A BC 0.945±0.793 A BC 1.841±1.745 A 0.01 0±0 BAC 0.232±0.205 BA BA 0.695±0.436 BA BA 1.121±0.666 BA BA 2.069±1.087 BA 0.05 0±0 A 0.288±0.250 A BA 0.680±0.382 BA A 1.336±0.709 A A 2.655±1.597 BA 0.001 0±0 BDC 0.118±0.196 BAC C 0.195±0.364 BA D 0.195±0.188 DC D 0.195±0.206 D 0.01 0±0 D 0.083±0.184 BAC C 0.172±0.357 BA D 0.172±0.357 BDC D 0.176±0.327 DC 0.05 0±0 BDC 0.139±0.207 BA C 0.229±0.347 BA D 0.229±0.353 BDC D 0.268±0.367 DC - **±** **±NS ***±*** ***±*** - 152 -

0 0±0 E 0.087±0.129 H E 0.557±0.384 E E 1.2105±0.757 BC G 2.247±1.333 B 1 0±0 CD 0.164±0.188 EF DE 0.670±0.431 DE ED 1.356±0.781 BC FDE 3.112±1.314 B 5 0±0 CB 0.195±0.229 CB DEC 0.763±0.581 BDAC BDC 1.595±1.004 BAC FDE 3.133±1.634 B 10 0±0 C 0.186±0.173 GF BAC 0.886±0.673 A BA 1.786±1.257 A BAC 3.749±1.931 B 20 0±0 A 0.253±0.216 CD BA 1.018±0.604 BA BC 1.640±1.035 BAC BDAC 3.456±1.740 B 30 0±0 A 0.272±0.209 D BAC 0.926±0.505 BDEC BA 1.836±0.853 BC FDE 3.139±1.418 B 1 0±0 D 0.153±0.111 I E 0.597±0.431 DE ED 1.335±0.789 BC FDE 2.922±1.509 B 5 0±0 A 0.280±0.291 A BAC 0.904±0.633 BA BA 1.851±1.027 BAC BA 3.837±4.482 A 10 0±0 A 0.274±0.234 B BAC 0.960±0.607 BA A 1.947±0.876 BA BDAC 3.459±1.792 B 20 0±0 B 0.220±0.190 EF A 1.048±0.613 BA BA 1.739±0.952 BAC BDEC 3.357±1.652 B 30 0±0 CB 0.195±0.165 G BA 1.006±0.651 BA BA 1.861±1.096 BA FDEC 3.199±1.603 B 1 0±0 C 0.186±0.199 ED DEC 0.754±0.508 BDEC BAC 1.695±0.867 BC FEG 2.801±1.646 B 5 0±0 CD 0.182±0.144 H DEC 0.755±0.446 DEC BAC 1.668±0.726 c FEG 2.769±1.586 B 10 0±0 CB 0.198±0.180 GF BDC 0.826±0.417 E EDC 1.440±0.714 C FG 2.703±1.530 B 20 0±0 A 0.260±0.216 CD BAC 0.959±0.578 BDAC BA 1.836±1.076 BA A 3.985±4.899 A 30 0±0 A 0.252±0.173 GF BDAC 0.872±0.592 BAC BA 1.862±0.976 BAC FDE 3.145±1.696 B - ***± ***± ***± ***± - 153 -

0 0 33.3 74.0 78.7 88.7 27.3 70.2 1.000 0.001 0 40.0 80.7 82.7 84.7 24.7 93.5 1.333 0.01 0 40.0 82.0 84.0 88.0 25.1 90.0 1.282 0.05 0 29.3 56.0 62.7 64.7 25.4 204.0 2.907 0.001 0 50.7 84.7 86.0 92.0 22.9 68.8 0.980 0.01 0 52.7 88.0 88.7 94.0 22.7 65.4 0.932 0.05 0 56.0 88.7 88.7 92.0 21.9 58.5 0.835 0.001 0 24.7 26.7 28.7 34.0 20.3 1137.7 16.206 0.01 0 18.0 30.0 32.0 34.7 23.6 7032 100.170 0.05 0 22.7 38.7 42.0 48.7 25.2 746.77 10.638 - *** *** *** *** - 154 -

0 0 40.7 68.7 88.7 98.7 27.7 62.9 1.000 1 0 56.0 80.7 94.0 98.7 22.6 55.8 0.887 5 0 62.0 82.0 94.7 96.7 21.4 55.0 0.875 10 0 68.7 82.7 92.0 98.0 20.6 52.3 0.832 20 0 74.0 84.7 90.0 96.0 19.8 53.6 0.853 30 0 76.0 86.0 94.0 96.7 19.6 54.1 0.860 1 0 66.0 78.7 88.7 96.0 20.7 56.8 0.903 5 0 64.7 80.7 92.7 94.7 20.8 51.9 0.825 10 0 70.7 86.7 92.7 98.0 20.3 52.3 0.833 20 0 68.7 80.0 94.7 96.7 20.4 53.6 0.852 30 0 72.0 86.7 88.7 92.7 19.7 53.7 0.854 1 0 56.7 82.7 94.0 94.0 21.9 56.7 0.902 5 0 74.7 82.0 96.0 96.7 19.8 57.5 0.907 10 0 68.7 86.7 94.7 94.7 20.3 59.0 0.923 20 0 74.0 86.7 94.0 96.7 19.8 57.1 0.822 30 0 74.7 82.0 94.7 98.7 19.9 53.9 0.858 - *** ** NS NS - 155 -

0 0±0 F 0.092±0.137 G E 0.215±0.219 E DC 0.757±0.613 BA ED 1.324±1.681 A 0.001 0±0 D 0.128±0.148 F D 0.299±0.231 D BAC 1.037±0.665 A BAC 2.204±1.249 BA 0.01 0±0 B 0.190±0.166 C B 0.460±0.236 D DC 0.768±0.388 BA EDC 1.549±1.115 BAC 0.05 0±0 E 0.103±0.119 I E 0.240±0.185 F ED 0.522±0.357 BA EF 0.812±0.567 BDC 0.001 0±0 A 0.229±0.171 B A 0.535±0.237 D BDC 0.937±0.465 BA BDC 1.920±1.443 A 0.01 0±0 C 0.144±0.134 H C 0.348±0.194 F A 1.415±0.652 BA BA 2.451±1.709 A 0.05 0±0 A 0.226±0.235 A A 0.546±0.351 A BA 1.269±0.608 BA A 2.810±1.100 BAC 0.001 0±0 FE 0.099±0.152 E E 0.220±0.252 C E 0.181±0.314 B F 0.181±0.320 D 0.01 0±0 H 0.069±0.154 ED G 0.130±0.265 B E 0.162±0.315 B F 0.195±0.423 DC 0.05 0±0 G 0.082±0.155 D F 0.172±0.258 CB E 0.200±0.318 BA F 0.523±0.532 BDC - ***± ***± ***± ***± - 156 -

0 0±0 F 0.067±0.116 I B 0.502±0.116 I C 1.059±0.567 A C 1.970±0.998 BC 1 0±0 ECD 0.136±0.185 GF B 0.609±0.185 GF BC 1.161±0.870 A C 1.988±0.930 BC 5 0±0 ECD 0.133±0.218 CD B 0.635±0.218 CD BAC 1.202±0.692 A BAC 2.300±1.334 A 10 0±0 ED 0.126±0.170 G B 0.597±0.170 G BC 1.168±0.618 A BC 2.036±0.916 BC 20 0±0 BA 0.232±0.223 CD BA 0.846±0.223 CD BAC 1.418±0.754 A BAC 2.405±1.137 BAC 30 0±0 A 0.255±0.215 CD BA 0.807±0.215 CD BAC 1.486±0.784 A BAC 2.266±1.230 BA 1 0±0 EF 0.113±0.092 J BA 0.768±0.092 J BAC 1.328±0.761 A BAC 2.241±0.830 C 5 0±0 A 0.264±0.293 A BA 0.699±0.293 A BAC 1.448±0.903 A BAC 2.205±1.002 BC 10 0±0 BA 0.235±0.244 B BA 0.799±0.244 B BAC 1.591±0.744 A BAC 2.371±0.921 BC 20 0±0 ECD 0.149±0.196 EF BA 0.799±0.196 EF BAC 1.501±0.639 A BAC 2.409±0.992 BC 30 0±0 ECD 0.140±0.174 G BA 0.743±0.174 G A 1.744±0.695 A BAC 2.453±0.982 BC 1 0±0 ECD 0.167±0.206 ED BA 0.763±0.206 ED A 1.734±0.833 A A 2.715±1.247 BA 5 0±0 ECD 0.164±0.149 H BA 0.736±0.149 H BAC 1.443±0.656 A BAC 2.481±1.135 BAC 10 0±0 BC 0.187±0.181 GF BA 0.713±0.181 GF BC 1.094±0.618 A BAC 2.138±0.812 C 20 0±0 BCD 0.182±0.229 CB A 1.049±0.229 CB BAC 1.598±0.731 A BAC 2.537±1.050 BAC 30 0±0 BA 0.237±0.172 G BA 0.781±0.172 G BA 1.632±0.655 A BA 2.594±1.039 BAC - ***±*** NS±*** *±NS NS±* - 157 -

0 0 26.0 66.0 78.7 82.0 28.5 84.8 1.000 0.001 0 38.0 78.0 84.7 96.0 27.0 63.0 0.743 0.01 0 44.0 84.0 90.0 94.0 24.9 74.6 0.879 0.05 0 38.0 78.0 78.0 86.7 25.6 129.8 1.530 0.001 0 50.0 86.7 88.0 90.0 22.8 67.0 0.790 0.01 0 46.0 86.0 94.0 92.0 24.0 60.5 0.713 0.05 0 46.0 86.0 90.7 92.7 24.1 57.5 0.678 0.001 0 26.0 30.0 34.7 36.0 20.3 1078.1 12.700 0.01 0 18.0 26.0 28.0 28.0 21.3 898.18 10.581 0.05 0 26.0 36.7 44.7 60.0 28.5 133.45 1.572 - *** *** *** *** - 158 -

0 0 32.0 74.7 90.7 90.7 27.8 66.9 1.000 1 0 46.7 80.7 90.0 92.7 23.9 67.4 1.007 5 0 42.0 80.7 88.7 90.0 24.9 62.1 0.928 10 0 46.7 84.0 92.0 92.7 23.9 66.4 0.993 20 0 68.0 84.7 92.0 92.7 20.2 61.1 0.913 30 0 72.0 82.0 88.7 88.7 19.4 63.6 0.951 1 0 62.0 94.0 94.0 96.7 21.4 63.4 0.948 5 0 62.7 90.0 92.7 94.0 21.0 64.6 0.966 10 0 60.7 88.0 88.7 94.7 21.4 61.9 0.925 20 0 46.0 88.0 92.0 92.7 24.1 61.2 0.915 30 0 58.0 90.0 92.7 92.0 21.6 60.7 0.907 1 0 48.7 94.0 92.7 94.7 23.7 57.3 0.857 5 0 68.0 88.7 92.0 92.7 20.2 60.2 0.900 10 0 66.7 84.0 88.7 94.0 20.5 64.1 0.958 20 0 50.0 92.0 94.7 96.7 23.6 59.5 0.889 30 0 72.7 90.7 92.7 96.7 20.0 58.8 0.879 - * ** NS * - 159 -

0 0 F 0.098±0.142 FE BAC 0.429±0.453 A C 1.178±0.550 B BA 2.132±0.952 A 0.001 0 0.01 0 0.05 0 0.001 0 0.01 0 0.05 0 0.001 0 0.01 0 0.05 0 D 0.137±0.159 C BEDC 0.316±0.276 C BA 1.442±0.531 B BA 2.184±0.868 A B 0.194±0.173 B BAC 0.413±0.304 BC BC 1.208±0.447 B B 1.669±0.612 B E 0.109±0.126 G EDC 0.266±0.222 C D 0.712±0.540 B C 0.735±0.583 B A 0.228±0.174 B BA 0.492±0.319 BAC BC 1.359±0.491 B A 2.237±0.808 A C 0.148±0.136 F BDC 0.366±0.234 C BC 1.402±0.754 BA A 2.364±0.836 A A 0.230±0.239 A A 0.602±0.431 BA A 1.652±0.682 BA A 2.359±0.906 A E 0.116±0.149 DC ED 0.187±0.318 BAC E 0.390±0.897 BA C 0.456±0.372 C H 0.069±0.154 DC E 0.137±0.317B AC E 0.241±0.985 BA C 0.326±0.276 C G 0.083±0.155 DC ED 0.199±0.303 BC E 0.447±1.384 A C 0.468±0.557 B - ***±*** **±* ***±NS ***±*** - 160 -

0 0±0 F 0.113±0.118 D H 0.535±0.387 B BC 1.254±0.481 B BA 2.656±0.976 BAC 1 0±0 BDC 0.230±0.179 C G 0.700±0.459 B C 1.215±0.549 BA BDAC 2.341±0.816 BDAC 5 0±0 BDAC 0.235±0.230 B G 0.691±0.480 B BA 1.517±0.575 BA BA 2.715±0.946 BDAC 10 0±0 DE 0.189±0.170 C EFD 0.848±0.607 B A 1.540±0.716 BA BA 2.715±0.875 BDAC 20 0±0 BAC 0.235±0.224 B ECD 0.897±0.590 B BAC 1.378±0.570 BA DC 2.048±0.670 BDC 30 0±0 BAC 0.246±0.181 C A 1.151±0.599 B BAC 1.366±0.512 B D 1.932±0.586 D 1 0±0 F 0.133±0.113 D FG 0.732±0.484 B BA 1.517±0.666 BA BA 2.705±0.792 BDAC 5 0±0 A 0.277±0.290 A EFD 0.829±0.509 B BAC 1.415±0.595 BA BDAC 2.454±0.819 BDAC 10 0±0 BA 0.261±0.224 B BA 1.079±1.067 A A 1.590±0.590 BA A 2.930±0.857B DAC 20 0±0 DEC 0.202±0.190 C BC 1.005±0.561 B A 1.531±0.528 B BDAC 2.37±0.618 DC 30 0±0 DEC 0.207±0.176 C BCD 0.959±0.648 B BA 1.483±0.535 B BDC 2.267±0.582 D 1 0±0 DEC 0.203±0.177 C EFG 0.789±0.500 B BAC 1.387±0.591 BA BDAC 2.490±1.149 A 5 0±0 E 0.175±0.174 C FG 0.755±0.495 B BA 1.486±0.626 BA BDAC 2.389±0.933 BDAC 10 0±0 BDC 0.222±0.179 C ECD 0.902±0.493 B BA 1.492±0.528 B BAC 2.558±0.798 BDAC 20 0±0 BAC 0.248±0.188 C CD 0.939±0.496 B A 1.584±0.980 A BA 2.681±0.726 BDC 30 0±0 A 0.276±0.169 C BC 1.001±0.524 B A 1.603±0.700 BA BA 2.816±1.003 BA - ***±*** **±** *±NS *±* - 161 -

0 0 26.0 62.7 94.0 96.7 31.3 64.6 1.000 0.001 0 38.0 66.7 98.0 98.0 28.6 65.1 1.007 0.01 0 44.0 68.0 94.7 98.0 26.5 81.6 1.263 0.05 0 38.0 64.7 74.0 76.7 24.2 980.8 15.177 0.001 0 50.0 80.0 96.7 100.0 24.0 63.5 0.984 0.01 0 46.0 88.0 90.7 96.7 24.7 61.6 0.955 0.05 0 46.0 86.0 94.0 96.7 24.7 62.3 0.965 0.001 0 40.7 32.0 50.7 54.0 20.0 174.0 6.679 0.01 0 18.0 24.0 48.7 52.0 37.0 260.1 4.025 0.05 0 26.0 40.0 48.7 50.7 23.4 1221.1 18.895 - *** *** *** ** - 162 -

0 0 68.0 86.0 96.7 100.0 20.8 58.6 1.000 1 0 78.7 87.3 93.3 96.7 19.4 61.6 1.051 5 0 64.7 90.0 98.0 86.0 21.1 57.8 0.986 10 0 62.7 88.7 96.0 98.0 21.4 57.8 0.986 20 0 66.0 88.0 95.3 96.0 20.7 68.0 1.160 30 0 74.7 92.0 94.7 96.7 19.8 72.0 1.229 1 0 66.0 89.3 94.7 97.3 20.8 57.9 0.988 5 0 65.3 86.7 94.7 98.0 21.0 60.5 1.032 10 0 71.3 88.7 96.0 98.0 20.2 56.1 0.957 20 0 66.7 92.0 97.3 98.0 20.8 61.8 1.055 30 0 76.0 91.3 95.3 98.0 19.7 63.5 1.084 1 0 67.3 86.0 92.0 92.7 20.3 60.1 1.026 5 0 64.0 85.3 92.7 94.0 20.8 61.4 1.048 10 0 76.0 91.3 96.0 97.0 19.7 59.3 1.012 20 0 74.7 91.3 95.3 98.0 19.9 58.0 0.990 30 0 82.7 91.3 94.7 96.0 19.0 56.8 0.969 - ** NS NS NS - 163 -

Amino acids BR GR CR CYA 1) 0.0 0.0 0.0 Aspartic acid 13.4 0.9 4.5 Glutamic acid 17.6 1.8 3.7 Asparagine 10.2 0.0 0.4 Serine 2.8 2.8 6.0 Glutamine 0.9 0.0 0.4 Histidine 1.6 1.2 1.2 Glycine 1.6 1.7 4.0 Arginine 4.3 3.1 1.3 Threonine 0.9 0.6 4.8 Alanine 7.2 4.5 5.2 GABA 2) 4.7 7.9 4.7 Proline 3.9 1.1 1.7 Theanine 3.5 1.8 3.1 Tyrosine 0.5 0.4 1.4 Valine 1.2 2.6 2.9 Methionine 0.0 0.3 0.8 Cystine 8.2 8.6 6.0 Isoleucine 4.8 4.9 4.5 Leucine 4.2 5.0 4.9 Phenylalanine 0.0 1.0 2.4 Tryptophan 34.6 40.1 41.2 Lysine 0.7 0.6 1.5 Total 126.8 90.9 106.6-164 -

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- 171 -

주 의 1. 이보고서는농림부에서시행한농림기술개발사업의연구보고서입니다. 2. 이보고서내용을발표할때에는반드시농림부에서시행한농림기술개발사업의연구결과임을밝혀야합니다. 3. 국가과학기술기밀유지에필요한내용은대외적으로발표또는공개하여서는아니됩니다.