한수지 49(6), , 2016 Original Article Korean J Fish Aquat Sci 49(6), ,2016 배합사료내야콘, 생강및블루베리첨가에따른조피볼락 (Sebastes schlegeli) 치어의성장, 혈액성상및 Strep

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1 한수지 49(6), , 2016 Original Article Korean J Fish Aquat Sci 49(6), ,2016 배합사료내야콘, 생강및블루베리첨가에따른조피볼락 (Sebastes schlegeli) 치어의성장, 혈액성상및 Streptococcus iniae 감염에미치는영향 이기욱 김희성 조성환 * 박찬일 1 하민수 2 한국해양대학교해양생명과학부, 경상대학교해양생명과학과 1, 대한사료 2 Effect of Dietary Inclusion of Yacon, Ginger and Blueberry on Growth, Feed Utilization, Serum Chemistry and Challenge Test against Streptococcus iniae of Juvenile Rockfish Sebastes schlegeli Ki Wook Lee, Hee Sung Kim, Sung Hwoan Cho*, Chan-Il Park 1 and Min-Su Ha 2 Division of Marine BioScience, College of Ocean Science and Technology, Korea Maritime University, Busan 49112, Korea 1 Department of Marine Biology and Aquaculture, Gyeongsang National University, Tongyeong 53064, Korea 2 Daehanfeed Co., Ltd., Incheon 22300, Korea Yacon (YC), ginger (GG) and blueberry (BB) were added to the diet of juvenile rockfish Sebastes schlegeli to investigate the effects of these substances on growth, feed utilization and resistance to Streptococcus iniae compared to feed additive ethoxyquin. A total of 480 fish were distributed in 12 tanks and four experimental diets [control (Con), with 0.01% ethoxyquin, and 1% YC, GG and BB diets] were provided in triplicate. After an 8-week feeding trial, 20 fish from each tank were infected with Streptococcus iniae. Fish fed the BB diet had higher weight gain than fish fed the GG and Con diets. Feed efficiency of fish fed the GG and YC diets was higher than that of fish fed the BB and Con diets. The protein efficiency ratio of fish fed the GG diet was higher than that of fish fed any other diet. The cumulative mortality 54 h after infection of fish fed the Con diet was higher than that of fish fed any other diet. These results indicate that YC, GG, and BB are effective dietary additive, not only improving growth performance and feed utilization but also lowering the mortality of juvenile rockfish exposed to S. iniae. Key words: Rockfish Sebastes schlegeli, Streptococcus iniae, Cumulative mortality 서론 (Sebastes schlegeli) (85,448 ) (45,759 : 53.6%) (18,774 : 22.0%) (KOSIS, 2016). (Kim et al., 2001; Cho et al., 2015), (Lee and Kim, 2009), (Lee, 2002), (Lee et al., 2000; Mizanur et al., 2014), (Lee et al., 1996; Jeon et al., 2014) lysozyme (Hwang et al., 2013) Vibrio alginolyticus (Kim et al., 1999).,.., Korean J Fish Aquat Sci 49(6) , December 2016 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Licens ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Received 6 October 2016; Revised 30 November 2016; Accepted 5 December 2016 *Corresponding author: Tel: Fax: address: chosunh@kmou.ac.kr Copyright 2016 The Korean Society of Fisheries and Aquatic Science 823 pissn: , eissn:

2 824 이기욱ㆍ김희성ㆍ조성환ㆍ박찬일ㆍ하민수 (WHO, 2006).. [Yacon (YC), Polymnia sochifolia Poeppig & Endlicher] polyphenol (Kim et al., 2010; Park et al., 2012), (Kim, 2013). Choi et al. (2010) Staphylococcus aureus. Kim et al. (2016) YC. [Ginger (GG), Zingiber officinale Roscoe] gingerols shogaol (Sebiomo et al., 2011), (Ekwenye and Elegalam, 2005) (Balestra et al., 2011). Akintobi et al. (2013) Salmonella typhi Proteus mirabilis,. (Nya and Austin, 2009; Talpur et al., 2013; Hassanin et al., 2014). [Blueberry (BB), Vaccinium ashei Reade] anthocyanin (Jeong et al., 2012; Vizzotto et al., 2013; Deng et al., 2014), (Dunlap et al., 2006; Molan et al., 2008; Papandreou et al., 2009).. ethoxyquin (6-ethoxy-2, 2, 4-trimethyl-1, 2-dihydroquinoline; EQ) (Thorisson et al., 1992), Wang et al. (2015) (Lateolabrax japonicus) EQ mg/kg diet. Streptococcus sp. (Choi et al., 2010; Jung et al., 2012). YC, GG BB, S. iniae EQ. 실험어및사육조건 재료및방법 2, 1 2 ( : 52%, 12%) 2-4%. [ (Mean SE: g)] L ( : 45 L) L/min, aeration. 실험사료의준비 Table 1.,.. 0.1% EQ (, ) (Con). 1% YC, GG BB (, ) 1%. 3: (09:00, 17:00). 일반성분분석,, AOAC (1990). Kjeldahl method (Auto Kjeldahl System, Buchi B-324/435/412, Switzerland) ether-extraction method, 550 C 4, 105 C dry oven 24. 채혈및혈액성상분석 8 1, 5 50 ppm,. Automatic Chemistry System (HITACHI 7180/ , Hitachi, Japan) total protein, total cholesterol, glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT) triglyceride.

3 배합사료내사료첨가제가조피볼락치어면역성에미치는영향 825 Table 1. Ingredients (%, DM basis) and nutrient composition of the experimental diets Ingredients (%) Experimental diets Con YC 1 GG 1 BB 1 Fish meal Fermented soybean meal Wheat flour Ethoxyquin Additives Squid liver oil Soybean oil Vitamin premix Mineral premix Choline Nutrients (%) Dry matter Crude protein Crude lipid Ash YC (Yacon), 1 GG (Ginger), and 1 BB (Blueberry) were purchased from Tojongherb Co Ltd. (Dongdaemun-gu, Seoul, Korea). 2 Fish meal and was purchased from Abank Co Ltd. (Seocho-gu, Seoul, Korea). 3 Fermented soybean meal was supplied by CJ CheilJedang Corp. (Jung-gu, Seoul, Korea). 4 Ethoxyquin was supplied from Chunhajeil feed Co Ltd. (Daedeok-gu, Daejeon, Korea). 5 Vitamin premix contained the following amount which were diluted in cellulose (g/kg mix): L-ascorbic acid, 121.2; DL-α-tocopheryl acetate, 18.8; thiamin hydrochloride, 2.7; riboflavin, 9.1; pyridoxine hydrochloride, 1.8; niacin, 36.4; Ca-D-pantothenate, 12.7; myo-inositol, 181.8; D-biotin, 0.27; folic acid, 0.68; p-aminobenzoic acid, 18.2; menadione, 1.8; retinyl acetate, 0.73; cholecalciferol, 0.003; cyanocobalamin, Mineral premix contained the following ingredients (g/kg mix): MgSO 4 7H 2 O, 80.0; NaH 2 PO 4 2H 2 O, 370.0; KCl, 130.0; ferric citrate, 40.0; ZnSO 4 7H 2 O, 20.0; Ca-lactate, 356.5; CuCl, 0.2; AlCl 3 6H 2 O, 0.15; KI, 0.15; Na 2 Se 2 O 3, 0.01; MnSO 4 H 2 O, 2.0; CoCl 2 6H 2 O, 1.0. S. iniae 에의한공격성실험 (challenge test) CFU/L S. iniae (FP5024) 0.1 ml. 7, ,. 통계분석 One-way ANOVA Duncan s multiple range test (Duncan, 1955) SAS version 9.3 program (SAS Institute, Cary, NC, USA). 결과및고찰 8, Table %. BB Con GG (P<0.05), YC (P>0.05). YC, GG BB Con (P<0.05). Kim et al. (2016) ( ) YC, GG BB. (Choi et al., 2004), (Kim et al., 2005), (Cho and Lee, 2012), (Cho et al., 2013),, (Yun et al., 2016). 8, Table 3. BB (YC, Con, GG) (P<0.05), GG (P<0.05). GG YC BB Con (P<0.05). GG BB, YC Con, Con (P<0.05). GG. GG Con 0.1-1% GG (Oncorhynchus mykiss), (Lates calcarifer) Nile tilapia (Oreochromis niloticus) ( ) (Nya and Austin, 2009; Talpur et al., 2013; Hassanin et al., 2014). 0.5% Chlorella (Bai et al., 2001) Lycium chinense (Lim et al., 2009)

4 826 이기욱ㆍ김희성ㆍ조성환ㆍ박찬일ㆍ하민수 Table 2. Survival (%), weight gain (g/fish) and specific growth rate (SGR) of rockfish Sebastes schlegeli fed the experimental diets containing various sources of additives for 8 weeks Experimental diets Initial weight (g/individual) Final weight (g/individual) Survival (%) Weight gain (g/individual) SGR 1 (%/day) Con 4.2± ±0.02 b 100.0±0.00 a 12.1±0.02 c 2.40±0.002 b YC 4.3± ±0.02 a 100.0±0.00 a 12.3±0.02 ab 2.43±0.002 a GG 4.3± ±0.02 a 100.0±0.00 a 12.2±0.02 b 2.42±0.003 a BB 4.3± ±0.02 a 100.0±0.00 a 12.3±0.03 a 2.43±0.006 a Values (means of triplicate±se) in the same column sharing a common superscript are not significantly different (P>0.05). YC, Yacon; GG, Ginger; BB, Blueberry. 1 SGR (%/day) = (Ln final weight of fish - Ln initial weight of fish) 100/days of feeding trial. Table 3. Feed consumption (g/fish), feed efficiency (FE) and protein efficiency ratio (PER) of rockfish Sebastes schlegeli fed experimental diets containing various sources of additives for 8 weeks Experimental diets Feed consumption (g/fish) FE (%) 1 PER 2 Con 11.6±0.03 b 104±0.2 b 2.06±0.004 c YC 11.6±0.06 b 106±0.5 a 2.08±0.009 b GG 11.5±0.03 c 107±0.2 a 2.13±0.003 a BB 11.8±0.03 a 105±0.1 b 2.09±0.003 b Values (means of triplicate±se) in the same column sharing a common superscript are not significantly different (P>0.05). YC, Yacon; GG, Ginger; BB, Blueberry. 1 Feed efficiency (FE, %) = Weight gain of fish 100/feed consumed. 2 Protein efficiency ratio (PER) = Weight gain of fish/protein consumed.. 8 total protein, total cholesterol, GPT, GOT triglyceride Table 4. (P>0.05)., (Jeon et al., 2013) S. iniae, 7 Fig Con GG Cumulative mortality (%) Elapsed time (day) Con YC GG BB Fig. 1. Cumulative mortality of rockfish Sebastes schlegeli fed the experimental diets containing various sources of additives for 8 weeks, and then infected by Streptococcus iniae (means of triplicates±se)., 54 ( 7 ) YC, GG BB Con (P<0.05). S. iniae,, (Baeck et al., 2006). YC, BB GG 7 S. parauberis Kim et al. (2016). EQ, Kim et al. (2016) Table 4. Serum chemistry of rockfish Sebastes schlegeli fed experimental diets containing various sources of additives for 8 weeks Experimental diets Total protein (g/dl) Total cholesterol (mg/dl) GPT (IU/L) GOT (IU/L) Triglyceride (mg/dl) Con 4.16±0.061 a 212.0±1.73 a 1.7±0.33 a 45.0±5.86 a 290.3±20.28 a YC 3.81±0.284 a 184.7±18.22 a 2.3±0.88 a 39.3±11.46 a 246.0±45.62 a GG 4.29±0.144 a 206.7±5.24 a 1.3±0.33 a 53.7±7.22 a 275.7±5.21 a BB 4.29±0.115 a 205.7±13.42 a 2.3±0.67 a 39.3±2.33 a 240.3±21.18 a Values (means of triplicate±se) in the same column sharing a common superscript are not significantly different (P>0.05). YC, Yacon; GG, Ginger; BB, Blueberry.

5 배합사료내사료첨가제가조피볼락치어면역성에미치는영향 827. YC, BB GG., Nile tilapia GG, Aeromonas hydrophila, V. harveyi A. hydrophila (Nya et al., 2009; Talpur et al., 2013; Hassanin et al., 2014)., 4 7 YC BB GG (P<0.05). GG YC BB S. iniae. (Sivaram et al., 2004; Ji et al., 2007a; Punitha et al., 2008; Kim et al., 2012; Talpur and Ikhwanuddin, 2012; Talpur et al., 2013; Talpur, 2014), Ji et al. (2007b) 2:2:1:1 Massa medicate fermentata, Crataegi fructus, Artemisia capillaries Cnidium officinale 0.3, 0.5 1% ( ) 0.1% ( )., YC, GG BB S. iniae (immunostimulant). 사사 2014 ( ),. " ( )",. References Akintobi OA, Onoh CC, Ogele JO, Idowu AA, Ojo OV and Okonko IO Antimicrobial activity of Zingiber officinale (ginger) extract against some selected pathogenic bacteria. Nat Sci 11, Association of Official Analytical Chemists (AOAC) Official Methods of Analysis, 15th edn. Arlington, VA, USA. Baeck GW, Kim JH, Gomez DK and Park SC Isolation and characterization of Streptococcus sp. from diseased flounder (Paralichthys olivaceus) in Jeju island. J Vet Sci 7, Bai SC, Koo J, Kim K and Kim S Effects of Chlorella powder as a feed additive on growth performance in juvenile Korean rockfish, Sebastes schlegeli (Hilgendorf). Aquacult Res 32 (Suppl. 1), Balestra F, Cocci E, Pinnavaia G and Romani S Evaluation of antioxidant, rheological and sensorial properties of wheat flour dough and bread containing ginger powder. LWT Food Sci Technol 44, org/ /j.lwt Cho SH and Lee S Onion powder in the diet of the olive flounder, Paralichthys olivaceus: Effects on the growth, body composition, and lysozyme activity. J World Aquacult Soc 43, x. Cho SH, Jeon GH, Kim HS, Kim DS and Kim C Effects of dietary Scutellaria baicalensis extract on growth, feed utilization and challenge test of olive flounder (Paralichthys olivaceus). Asian-Aust J Anim Sci 26, org/ /ajas Cho SH, Kim HS, Myung SH, Jung W, Choi J and Lee S Optimum dietary protein and lipid levels for juvenile rockfish (Sebastes schlegeli, Hilgendorf 1880). Aquacult Res 46, Choi S, Ko S, Park G, Lim S, Yu G, Lee J and Bai SC Utilization of song-gang stone as dietary additive in juvenile olive flounder, Paralichthys olivaceus. J Aquaculture 17, Choi HS, Jee B, Cho MY and Park M Monitoring of pathogens on the cultured Korean rockfish Sebastes schlegeli in the marine cages farms of south sea area from 2006 to J Fish Pathol 23, Choi JG, Kang OH, Lee YS, Oh YC, Chae HS, Obiang-Obounou B, Park SC, Shin DW, Hwang BY and Kwon DY Antimicrobial activity of the constituents of Smallanthus sonchifolius leaves against methicillin-resistant Staphylococcus aureus. Eur Rev Med Pharmacol Sci 14, Deng Y, Yang G, Yue J, Qian B, Liu Z, Wang D, Zhong Y and Zhao Y Influence of ripening stages and extracting solvents on the polyphenolic compounds, antimicrobial and antioxidant activities of blueberry leaf extracts. Food Contr 38, Duncan DB Multiple range and multiple F tests. Biometrics 11, Dunlap KL, Reynolds AJ and Duffy LK Total antioxidant power in sled dogs supplemented with blueberries and the comparison of blood parameters associated with exercise. Comp Biochem and Physiol A Mol Integr Physiol 143, 429-

6 828 이기욱ㆍ김희성ㆍ조성환ㆍ박찬일ㆍ하민수 Ekwenye UN and Elegalam NN Antibacterial activity of ginger (Zingiber officinale Roscoe) and garlic (Allium sativum L.) extracts on Escherichia coli and Salmonella typhi. Int J Mol Med Adv Sci 1, Hassanin ME, Hakim Y and Badawi ME Dietary effect of ginger (Zingiber officinale Roscoe) on growth performance, immune response of Nile tilapia (Oreochromis niloticus) and disease resistance against Aeromonas hydrophila. Abbassa Int J Aqua 7, Hwang J, Lee S, Rha S, Yoon H, Park E, Han K and Kim S Dietary green tea extract improves growth performance, body composition, and stress recovery in the juvenile black rockfish, Sebastes schlegeli. Aquacult Inter 21, Jeon GH, Cho SH, Lee SM, Nam TJ and Kim DS Effects of the dietary inclusion of Porphyra and sea tangle Laminaria japonica on the growth, feed utilization, body composition, and plasma chemistry of juvenile Korean rockfish Sebastes schlegelei. Korean J Fish Aquat Sci 46, Jeon GH, Kim HS, Myung SH and Cho SH The effects of the dietary substitution of fishmeal with tuna by-product meal on growth, body composition, plasma chemistry and amino acid profiles of juvenile Korean rockfish (Sebastes schelgeli). Aquacult Nutr 20, org/ /anu Jeong H, Jo Y, Jeong J, Kim H and Heo HJ Nutritional composition and in vitro antioxidant activities of blueberry (Vaccinium ashei) leaf. Korean J Food Preserv 19, Ji SC, Takaoka O, Jeong GS, Lee SW, Ishimaru K, Seoka M and Takii K. 2007a. Dietary medicinal herbs improve growth and some non-specific immunity of red sea bream Pagrus major. Fish Sci 73, Ji SC, Jeong G, Im GS, Lee SW, Yoo JH and Takii K. 2007b. Dietary medicinal herbs improve growth performance, fatty acid utilization, and stress recovery of Japanese flounder. Fish Sci 73, Jung SH, Choi H, Do J, Kim MS, Kwon M, Seo JS, Hwang JY, Kim SR, Cho Y, Kim JD, Park MA, Jee B, Cho MY and Kim JW Monitoring of bacteria and parasites in cultured olive flounder, black rockfish, red sea bream and shrimp during summer period in Korea from 2007 to J Fish Pathol 25, jfp Kim Y Effect of dietary supplementation of yacon (Polymnia sonchifolia) by-product on performance and physicochemical properties of chicken thigh meat. Kor J Poult Sci 40, Kim KH, Hwang YJ and Bai SC Resistance to Vibrio alginolyticus in juvenile rockfish (Sebastes schlegeli) fed diets containing different doses of aloe. Aquaculture 180, Kim K, Wang XJ and Bai SC Reevaluation of the optimum dietary protein level for the maximum growth of juvenile Korean rockfish, Sebastes schlgeli (Hilgendorf). Aquacult Res 32 (Suppl. 1), org/ /j x x. Kim C, Kang B, Jee H, Choi A, Kim C, Cho Y, Hahn H and Nam KD Effect of chitosan-based feed additive on the growth and quality of cultured Japanese flounder, Paralichthys olivaceus. J Chitin Chitosan 10, Kim AR, Lee JJ, Jung HO and Lee MY Physicochemical composition and antioxidative effects of yacon (Polymnia sonchifolia). J Life Sci 20, Kim S, Harikrishnan R, Kim M, Balasundaram C and Heo M Broussonetia kazinoki as a feed additive enhances disease resistance against Streoptococcus parauberis in Paralichthys olivaceus. Fish Pathol 47, Kim HS, Kim HJ, Choi DG, Jang B, Cho SH, Kwon M, Min B and Kim DS Effect of various source of dietary additives on growth, body composition, and challenge test survival of juvenile rockfish Sebastes schlegeli. Turk J Fish Aquat Sci 16, KOSIS Korean Statistical Information Service. Korea. 16, Lee SM Apparent digestibility coefficients of various feed ingredients for juvenile and grower rockfish (Sebastes schlegeli). Aquaculture 207, org/ /s (01) Lee S, Yoo J and Lee JY The use of soybean meal, corn gluten meal, meat meal, meat and bone meal, or blood meal as a dietary protein source replacing fish meal in Korean rockfish (Sebastes schlegeli). Kor J Anim Nutr Feedstuffs 20, Lee S, Hwang U and Cho SH Effects of feeding frequency and dietary moisture content on growth, body composition and gastric evacuation of juvenile Korean rockfish (Sebastes schlegeli). Aquaculture 187, org/ /s (00) Lee S and Kim K Effects of dietary carbohydrate to lipid ratios on growth and body composition of juvenile and grower rockfish, Sebastes schlegeli. Aquacult Res 40, Lim DK, Yoo KY, Shin DG, Kim JE, Bae J, Bai SC and Lee JY Effects of dietary kugija Lycium chinense supplementation on juvenile Korean rockfish Sebastes schlegeli. Korean J Fish Aquat Sci 42, kfas Mizanur RM, Yun H, Moniruzzaman M, Ferreira F, Kim K and

7 배합사료내사료첨가제가조피볼락치어면역성에미치는영향 829 Bai SC Effects of feeding rate and water temperature on growth and body composition of juvenile Korean rockfish, Sebastes schlegeli (Hilgendorf 1880). Asian-Australas J Anim Sci 27, ajas Molan AL, Lila MA and Mawson J Satiety in rats following blueberry extract consumption induced by appetitesuppressing mechanisms unrelated to in vitro or in vivo antioxidant capacity. Food Chemistry 107, dx.doi.org/ /j.foodchem Nya EJ and Austin B Use of dietary ginger, Zingiber officinale Roscoe, as an immunostimulant to control Aeromonas hydrophila infections in rainbow trout, Oncorhynchus mykiss (Walbaum). J Fish Dis 32, org/ /j x. Papandreou MA, Dimakopoulou A, Linardaki ZI, Cordopatis P, Klimis-Zacas D, Margarity M and Lamari FN Effect of a polyphenol-rich wild blueberry extract on cognitive performance of mice, brain antioxidant markers and actylcholinesterase activity. Behav Brain Res 108, dx.doi.org/ /j.bbr Park JS, Kim HS and Chin KB The antioxidant activity of yacon (Polymnia sonchifolia) and its application to the pork patties as a natural antioxidant. Korean J Food Sci Ani Resour 32, Punitha SMJ, Babu MM, Sivaram V, Shankar VS, Dhas SA, Mahesh TC, Immanuel G and Citarasu T Immunostimulating influence of herbal biomedicines on non-specific immunity in grouper Epinephelus tauvina juvenile against Vibrio harveyi infection. Aquacult Int 16, dx.doi.org/ /s Sebiomo A, Awofodu AD, Awosanya AO, Awotona FE and Ajayi AJ Comparative studies of antibacterial effect of some antibiotics and ginger (Zingiber officinale) on two pathogenic bacterial. J Microbiol Antimicrob 3, Sivaram V, Babu MM, Immanuel G, Murugadass S, Citarasu T and Marian MP Growth and immune response of juvenile greasy groupers (Epinephelus tauvina) fed with herbal antibacterial active principle supplemented diets against Vibrio harveyi infections. Aquaculture 237, dx.doi.org/ /j.aquaculture Talpur AD and Ikhwanuddin M Dietary effects of garlic (Allium sativum) on haemato-immunological parameters, survival, growth, and disease resistance against Vibrio harveyi infection in Asian seabass, Lates calcarifer (Bloch). Aquaculture , aquaculture Talpur AD, Ikhwanuddin M and Bolong AA Nutritional effects of ginger (Zingiber officinale Roscoe) on immune response of Asian sea bass, Lates calcarifer (Bloch) and disease resistance against Vibrio harveyi. Aquaculture , Talpur AD Mentha piperita (Peppermint) as feed additive enhanced growth performance, survival, immune response and disease resistance of Asian seabass, Lates calcarifer (Bloch) against Vibrio harveyi infection. Aquaculture , Thorisson S, Gunstone FD and Hardy R The antioxidant properties of EQ and of some of its oxidation products in fish oil and meal. J Amer Oil Chem Soc 69, dx.doi.org/ /bf Vizzotto M, da Rosa Fetter M, Corbelini DD, Pereira MC amd Gonzales TN Bioactive compounds and antioxidant activity of blueberry (Vaccinium ashei Reade). Acta Horticult 972, Wang J, Ai Q, Mai K, Xu H, Zuo R, Xu W, Zhang W and Zhang C Effects of dietary ethoxyquin on growth, feed utilization and residue in the muscle of juvenile Japanese seabass, Lateolabrax japonicus. Aquacult Res 46, World Health Organization (WHO) Report of a joint FAO/OIE/WHO expert consultation on antimicrobial use in aquaculture and antimicrobial resistance. Seoul, Republic of Korea. Yun A, Kim HS, Seo Y, Cho SH and Bae JY Effects of dietary antioxidant supplementation on the growth, serum chemistry, body composition and challenge test results of juvenile rockfish Sebastes schlegeli. Korean J Fish Aquat Sci 49,

324 윤아영ㆍ김희성ㆍ서영완ㆍ조성환ㆍ배준영 ( ),. (herb), (Turan and Akyurt, 2005; Turan et al., 2007; Galina et al., 2009; Soosean et al., 200; Dada and Oviawe, 20; Deng

324 윤아영ㆍ김희성ㆍ서영완ㆍ조성환ㆍ배준영 ( ),. (herb), (Turan and Akyurt, 2005; Turan et al., 2007; Galina et al., 2009; Soosean et al., 200; Dada and Oviawe, 20; Deng 한수지 49(3), 323-329, 206 Original Article Korean J Fish Aquat Sci 49(3),323-329,206 항산화기능사료첨가제가조피볼락 (Sebastes schlegelii) 의성장, 체조성, 혈액성상및세균공격성에미치는영향 윤아영 김희성 서영완 조성환 * 배준영 한국해양대학교해양생명과학부, CJ 제일제당 Effects