Conventional PCR: AllInOneCycler Real-Time PCR: Exicycler 96 Protein Synthesis and Purification: ExiProgen DNA/RNA Preparation: ExiPrep 16 Plus & ExiC

Conventional PCR: AllInOneCycler Real-Time PCR: Exicycler 96 Protein Synthesis and Purification: ExiProgen DNA/RNA Preparation: ExiPrep 16 Plus & ExiCracker Electrophoresis: Agaro-Power Microbial Culture: HT-MegaGrow Vortexing & Spin-down: ExiSpin Instruments & Devices Phone: 1588-9788 (ext.4->3) Email: Instrument-support @bioneer.co.kr

01 Conventional PCR AllInOneCycler PCR System 3

AllInOneCycler PCR System Features and Benefits Fastest ramp rate AllInOneCycler TM 에적용된일반 thermal block 의 ramp rate 는최대 6.5 /sec 이며, 특수합금 thermal block* 은최대 9.5 / sec 로향상되었습니다. 이로인해전체적인실험시간이단축되어실험자의소중한시간과에너지를절약할수있습니다. 특수합금 thermal block 은기존의 aluminum block 에비해열용량이 30% 작은재질로제작되어 ramp rate 를약 30% 증가시켰습니다. * 특수합금 thermal block: 바이오니아에서자체개발한물질로제작된 thermal block 으로특허를취득하였으며, 미국, 유럽, 일본, 중국에특허출원중에있습니다 Description AllInOneCycler TM 는바이오니아의특허기술로자체개발한특수재질의 thermal block 과독창적인온도제어알고리즘을적용하여 ramp rate 를획기적으로증가시킨유전자증폭기기입니다. 고객의실험분야및목적에따라 6 종류의 block 을제공하고있어사용자의편의성을대폭향상시켰습니다. 온도변화량 (delta T) = 열량 (Q) 비열 (C) * 비중 (M) 열량 (Q) 이일정할경우온도변화량 (delta T) 을높이기위해서는비열 (C)* 비중 (M) 의값이감소되어야함. AllInOneCycler TM 는독창적인온도제어알고리즘으로정밀한온도제어가가능해재현성있는결과를얻을수있으며, gradient 기능을이용하여한번에최적의실험조건을찾을수있습니다. 그리고편의성을강조한사용자인터페이스가적용되어장비의사용이쉬워졌으며, 컴팩트한크기와경량화설계로공간효율성과이동성을높였습니다. Figure 1. Thermal block 금속에따른열용량비교. www.bioneer.co.kr 3

AllInOneCycler PCR System 실제 PCR 실험을통해, 타사장비에비해약 10 % 의구동시간절감효과를확인하였습니다. Figure 2. AllInOneCycler TM ( 일반 block 장착 ) 과경쟁사장비의구동시간및 PCR 결과비교. 높은에너지효율성 Thermal block에새로운합금을사용하여기존의일반 thermal block에비해열용량이약 30% 낮아졌습니다. 열용량이낮기때문에합금 thermal block을사용할경우에타사의장비에비해약 20% 의에너지절감효과가확인되었습니다. (a) (b) Figure 3. (a) 일반 block 과합금 block 의열용량비교. (b) AllInOneCycler TM ( 합금 block 장착 ) 와경쟁사장비의 PCR 1 회시전력소모량비교. 높은온도균일성과정확성더욱정밀해진온도제어기능으로, 온도균일성과정확성이향상되어재현성높은결과를얻을수있습니다. 이를통해중앙부와주변부의온도차이가발생하는기존장치들의문제점을극복하였습니다. BIONEER Figure 4. 96-well block 과 384-well block 의 24 개 well 에서 PCR 결과비교. 4

AllInOneCycler PCR System Interchangeable thermal block system: AllInOneCycler TM 는재질에따라일반 thermal block 과특수합금 thermal block 을선택적으로사용할수있으며, 샘플에따라 96-well, 384- well, slide block 을선택할수있습니다. 총 6 가지 block 에서실험목적에맞게 block 을교체하여사용할수있으며, 신규개발 block 을계속추가할예정입니다. Figure 5. AllInOneCycler TM 의교체가능한 thermal block. Thermal gradient PCR: AllInOneCycler TM 에탑재된 gradient 기능을이용해실험자는한번에최적의실험조건을쉽게찾을수있어새로운실험조건설정시시간과소모품비용을최소화할수있습니다. Figure 6. AllInOneCycler TM 의 thermal gradient PCR 기능을이용한 PCR 조건설정 (Human genomic DNA, CFS2 gene 830 bp). 간편한사용자인터페이스 : 7인치의터치스크린 LCD 채용과사용자중심의 UI를통해편리하고쉽게장비를사용할수있으며, 8종의 template 프로토콜을통해실험프로토콜을설정하는시간을줄일수있습니다. 또한, PC에서다수의장비를제어하고모니터링하는별도의프로그램을통해동시에대량의샘플을처리할수있습니다. (a) 시작화면 (b) Protocol 작성및수정화면 (c) 기존 protocol 불러오기화면 Figure 7. AllInOneCycler TM 의화면구성. 컴팩트하고심플한디자인 : 심플하고세련된디자인과작고가벼운사이즈로공간이좁은소규모실험실에서도쉽게설치할수있으며, 위치이동이용이합니다. Figure 8. AllInOneCycler TM 의앞, 뒤, 측면디자인. www.bioneer.co.kr 5

AllInOneCycler PCR System Specifications Physical specifications Dimension (W x H x D) 246 x 232 x 369 mm (9.68 x 9.14 x 14.53 in) Weight 8.4 kg (18.52 lbs) Display 7-inch Touch LCD screen Control Embedded Board Power consumption 100-240 V~, 8.5 A max, 50/60 Hz, 630 VA Operating temperature 15~30 C (59~86 F) Operating humidity 20~80 %, no condensation Port 2 USB ports for data storage Operating specifications Method of heating / cooling Peltier Temperature range 4.0 C~99.9 C (39.2 F ~ 211.82 F) Gradient range 20 C~95 C Block type Fast Block Normal Block Max heating ramp rate 9.5 C/sec (17.1 F/sec) 6.5 C/sec (11.7 F/sec) Max cooling ramp rate 7.7 C/sec (13.86 F/sec) 4.5 C/sec (8.1 F/sec) Temperature accuracy ± 0.3 C (±0.54 F) Temperature uniformity < 0.3 C ( 0.54 F) Lid temperature 90~110 C (194 F ~230 F) Ramp rate control 1~100% Ordering Information BIONEER Cat. no. Product Description AllInOneCycler TM PCR System A-2041-1N AllInOneCycler TM 96 well PCR system A-2041-2N AllInOneCycler TM 384 well PCR system A-2041-3N AllInOneCycler TM Slide PCR system AllInOneCycler TM Fast PCR System A-2041-1F AllInOneCycler TM Fast 96 well PCR system A-2041-2F AllInOneCycler TM Fast 384 well PCR system A-2041-3F AllInOneCycler TM Fast Slide PCR system AllInOneCycler TM Thermal Block A-2041-1-1 AllInOneCycler TM 96 well thermal block only A-2041-2-1 AllInOneCycler TM 384 well thermal block only A-2041-3-1 AllInOneCycler TM Slide thermal block only A-2041-1-2 AllInOneCycler TM Fast 96 well thermal block only A-2041-2-2 AllInOneCycler TM Fast 384 well thermal block only A-2041-3-2 AllInOneCycler TM Fast Slide thermal block only AllInOneCycler TM PC Control Software A-2041-9 AllInOneCycler TM PC control software DNA Polymerase & PCR PreMix K-2012 AccuPower PCR PreMix, 96 tube K-2631 AccuPower ProFi Taq PCR PreMix, 96 tubes K-2111 AccuPower Multiplex PCR PreMix, 96 tubes 6

AllInOneCycler PCR System K-2611 AccuPower PyroHotStart Taq PreMix, 96 tubes Reverse Transcriptase & RT-PCR PreMix K-2044 AccuPower CycleScript RT PreMix, dt20, 96 tubes K-2201 AccuPower RocketScript TM Cycle RT PreMix, 96 tubes K-2501 AccuPower RocketScript TM RT-PCR PreMix, 96 tubes Plastic consumables TC2-02-N 0.2 ml Flat Cap PCR Tube, 1000 tubes T-028-CN 0.2 ml PCR 8-Tube Strip with 8-Cap Strip T-0212-CN 0.2 ml PCR 12-Tube Strip with 12-Cap Strip 3247-01 0.2 ml PCR Tubes with individual attached UltraFlux I 3420-00 Semi skirt 96-Well PCR Cycle Plate, 10 plates 3430-00 Full skirt 384-Well PCR Cycle Plate, 10 plates 3510-00 96 well sealing mat, 5 ea www.bioneer.co.kr 7

02 Real-Time PCR Exicycler 96 (Ver.4) Real-Time Quantitative Thermal Block 9

Exicycler 96 (Ver.4) Real-Time Quantitative Thermal Block Conventional Optics Light (Unpolarized) Bioneer s patented polarizing optics Light (Unpolarized) Band pass filter Band pass filter Polarizer Dichromic mirror Band pass filter Noise signal Detector Polarizing Beam filter Polarizer Band pass filter Minimized noise Detector Figure 1. Separation of a reflection light from fluorescence. A reflection light (Unpolarized) A reflection light (Polarlized) Description Exicycler TM 96 (Ver.4) 는 96 well 동시검출방식의 Real-Time qpcr 장비입니다. 바이오니아의특허인 LT (Light Tunnel) 기술과편광을이용한형광검출기술로광학적민감도와정확도를높이고 96 well 간의편차를최소화하여 reference dye 에의한 normalization 이없이각 well 당 5 채널의형광값을정밀하게분석할수있습니다. 또한최적의 PCR annealing 온도를한번에찾을수있는 thermal gradient block 기능으로실험조건설정시시간과소모품비용을최소화할수있습니다. 분석소프트웨어는 Absolute Quantification, Relative Quantification, SNP genotyping, Existence/Nonexistence 모드를제공하여간편하게 viral load analysis, 유전자발현분석, SNP genotyping 등다양한분야의실험분석이가능합니다. Features and Benefits 민감하고정확한실험을위한최고의광학계편광을이용한형광검출기술로반사광에의한노이즈 * 를최소화하여실험민감도와정확도가향상되었습니다 (Figure 1). * 반사광에의한노이즈 : qpcr에이용되는형광물질은여기광 (Excitation energy) 을받아여기상태가되었다가기저상태로떨어지면서형광을발하게됩니다 (emission). 일반적인 qpcr 장비에서는형광물질 (fluorescence dye, reporter dye 등 ) 을여기시키기위해시료로조사된여기광의일부가 plate 및기구내부표면에서반사되면서시료에서발생된형광과함께형광검출장치로입사됩니다. 일반적으로형광필터 (band pass filter) 로반사광을제거하게되지만, 여기광의광량이형광량에비해아주크기때문에, 이에비례하여반사광의광량또한상당히커서형광필터로완벽하게제거하지못하여시료의형광검출시제거되지못한반사광이노이즈를발생시켜민감도와정확도를떨어뜨리는요인으로작용합니다. Figure 1. Separation of a reflection light from fluorescence. Reference dye 사용이불필요하여 5-multiplex PCR 가능 Well 간광학편차를기구적으로해결하는바이오니아의 LT 기술로일반적으로편차보정을위해사용되는 reference dye 가필요하지않으므로 5 채널의형광을모두시료에활용할수있어 real 5 multiplex PCR 이가능합니다. Excitation Emission Fluorescence Dye 1 475 nm 530 nm FAM, SYBR Green I 2 520 nm 560 nm JOE, TET 3 550 nm 590 nm TAMRA, CY3 4 570 nm 630 nm Texas Red, ROX, Red 610 5 630 nm 690 nm CY5 Ramp rate 향상으로반응시간단축바이오니아의 thermal block과온도제어기술로온도조절에대한 ramp rate가향상되어, PCR 반응에걸리는시간 (run time) 을 40% 이상줄였습니다 ( 당사기존모델대비 ) (Figure 2). Time (min) 100 80 60 40 20 Reducing run time Cycling time Ramping time 0 Previous model Newest model Figure 2. Comparison of operating time and ramping time between the previous model and the newest model of Exicycler TM 96. www.bioneer.co.kr 9

Exicycler 96 (Ver.4) Real-Time Quantitative Thermal Block 정확한온도제어와 Ct 값오차최소화로높은실험재현성구현 정확한온도제어를통해전체 well에대한온도정확성과균일성을향상시켰습니다. 온도편차가 ±0.3 이내로가장자리와중앙의온도차이가매우작습니다 (Figure 3). 또한빠르고정확한분석을위해모든 well의형광 data를 2D CCD sensor를통해동시에측정하여 96 well plate의 well간의측정시간차이에따른편차 (Time-lag) 를없앴습니다. 광학계에 LT 특허기술을적용하여모든 well에조사되는빛의양이균일하기때문에, 어떤 well에서 PCR 하여도 Ct 값편차가 0.3 cycle 이내로유지됩니다 (Figure 4). Fluorescence graph Melting curve analysis Standard curve analysis Relative Quantification Figure 3. Exicycler TM 96 has superior well-to-well uniformity. Existence/non-existence assay Figure 5. Exicycler TM 96 Analysis Software. SNP Genotyping Wide Linear Dynamic Range로저농도및고농도의시료정량에탁월 Detection range는 10 1 ~10 9 copies 이상으로저농도및고농도의시료에서도 DNA 정량, 정성분석이가능합니다 (Figure 6). Figure 4. qpcr result using 1x10 6 copy of IRF3 gene in each of 96 well positions. Figure 6. Ct values of 10-fold diluted samples show a wide dynamic range of quantification and high PCR efficiency. Self-diagnostics, Door 자동개폐기능탑재및사용자중심인터페이스로편의성증대시스템의고장유무를실험전에확인하는자체진단기능을보유하고있어, 실험진행중발생될문제점과그에따른시료의손실을사전에방지할수있습니다. Door 자동개폐기능으로작동이편리할뿐만아니라전자동화시스템에적용하기적합합니다. 또한 melting curve, absolute quantification, relative quantification, existence/nonexistance, SNP genotyping 결과를손쉽게분석할수있는 Exicycler TM 96 analysis software를제공하여사용자편의성을높였습니다 (Figure 5). BIONEER 10

Exicycler 96 (Ver.4) Real-Time Quantitative Thermal Block Temperature gradient function 으로빠르게최적의 PCR 조 건결정 40~75 범위에서 1~20 까지온도 gradient 가가능하여최적의 PCR annealing 온도를한번에찾을수있습니다. 폭넓은 application Time increment, Temp increment 조절기능을보유하고있어 touch down PCR 과같은 application 이가능합니다. Applications Quantification of Gene Expression MicroRNA Expression Analysis Gene Detection Virus Load Analysis Pathogen Detection Genotype Analysis SNP (Single nucleotide polymorphism) Detection Drug Resistance Analysis Genetic Disease Detection Oncology DNA Methylation Study Specifications Temperature Accuracy ± 0.3 (±0.54 ) Temperature Uniformity ± 0.3 (±0.54 ) Ramping Rate Fast block Normal block Max 5.0 (9.0 )/sec Max 4.5 (8.1 )/sec Lid Temperature 90~120 (194 ~ 248 ) Gradient Range 20~95 (36 ~ 203 ) Temperature Increment Range Time Increment Range 0.1~2.0 (0.18 ~ 3.6 ) 1 ~ 60 sec Ramp rate control range 1~100% Operating temperature 15 ~ 30 (59 ~ 86 ) Operating Humidity 20 ~ 80%, no condensation Computer Specifications Operating System Windows XP & Window7 (32-bit OS only) Processor Speed Intel Dual Core E2160 (1.8 GHz) or higher Memory Communication Port Screen Resolution 1 GB or higher USB 2.0 high speed 1280X1024 or higher * 본기기의사양은제품의성능개선을위해예고없이변경될수있습니다. Physical specifications Dimension (W x D x H) 355 x 540 x 470 mm Weight (kg) Sample Capacity/ Size Sample Volume Input voltage Frequency Power Optical Specifications Light Source Wattage Sensor 39 kg Fast block Normal block Fast block Normal block 100~240 VAC 50/60 Hz Opaque White 96-well Low Profile PCR Plate/0.1 ml Opaque White 8-strip Low profile PCR Tube Opaque White 96-well PCR Plate/0.2 ml Opaque White 8-strip PCR Tube 10 ~ 50 μl (20 μl recommended) 20 ~ 100 μl (50 μl recommended) 800 VA (Fuse: 250 V, F10AL, 2 ea) Short arc lamp 120 W 2D CCD Excitation Filter / 5 Set Emission Filter Operating Specifications Method of Heating / Peltier cooling Temperature Range 4.0~99.9 (39.2 ~ 211.82 ) www.bioneer.co.kr 11

Exicycler 96 (Ver.4) Real-Time Quantitative Thermal Block Ordering Information Cat. no. A-2060-1 A-2060-2 Product Description Exicycler 96 (ver.4) Real-Time Quantitative Thermal Block Exicycler 96 (Ver.4) Fast Real-Time Quantitative Thermal Block Related Products Cat. no. Accessories A-2060-A1 AccuPower Fluorescence Test Kit for Exicycler 96 A-2060-A2 AccuPower Fluorescence Test Kit for Exicycler 96 Fast Cat. no. Premix & Reagent K-6600 AccuPower Plus DualStar qpcr PreMix, 50 µl/rxn, 8-tube strips, 96 rxn, Exicycler, optical film included K-6210 AccuPower GreenStar qpcr PreMix, 50 µl/rxn, 8-tube strips, 96 rxn, Exicycler, optical film included K-6253 AccuPower 2X GreenStar qpcr Master Mix, 50 µl/rxn, 100 Rxn, K-6704 AccuPower Dual-HotStart RT-qPCR PreMix, 50 µl/rxn, 8-tube strips, 96 rxn, Exicycler, optical film included Cat. no. Plastic Consumables 3111-4110 Adhesive Optical Sealing Film, 100 sheets 3111-50 0.2 ml Opaque White 8-tube strip PCR Tube, 250 Strips 3111-51 0.2 ml Opaque White 8-tube strip PCR Tube, Low Profile, 250 Strips 3111-52 Opaque White 96-well Semi-skirted PCR Plate, 25 Plates 3111-53 Opaque White 96-well Semi-skirted, Low Profile PCR Plate, 25 Plates (Full-skirted) BIONEER 12

03 Protein Synthesis and Purification ExiProgen TM 14

Adapt or AC-I nput DC-Out put DC-I nput

ExiProgen Application Enzyme engineering Protein evolution Synthetic biology Bio-energy R&D Protein drug R&D Available Experiments with Bioneer s Kits Protein Synthesis/Purification Kit Genomic DNA Extraction Kit Total RNA Extraction Kit Viral DNA/RNA Extraction Kit Fragment DNA Purification Kit Procedure ( 단백질합성 ) Experimental Data 다양한크기의단백질합성결과 Figure 1. Plasmid DNA를 template로사용한경우 10~120 kda, PCR product를사용한경우는 10~60 kda까지합성이가능한것을확인. M1: AccuLadder TM Protein Size Marker (Low) 1: Calul3 (17.5 kda) 2: RNase H (20 kda) 3: DUSP 3 (22 kda) 4: CAT (24 kda) 5: AcGFP (29 kda) 6: EF-Ts (34 kda) 7: VF (45 kda) 8: Poly A polymerase (50 kda) 9: M-MLV RTase (75 kda) 10: BM3 (117 kda) M2: AccuLadder TM Protein Size Marker (Broad) Ordering Information Cat. no. Product Description A-5041 ExiProgen TM Cat. no. Protein synthesis and Purification Kits for ExiProgen TM K-7300 ExiProgen EC Protein Synthesis Kit, 16 rxns K-7301 ExiProgen EC Protein Synthesis Kit, 32 rxns K-7302 ExiProgen EC Protein Synthesis Kit, 96 rxns K-7310 ExiProgen EC-Maxi Protein Synthesis Kit, 8 rxns K-7320 ExiProgen EC-Tagfree Protein Synthesis Kit, 8 rxns K-7330 ExiProgen EC-Disulfide Protein Synthesis Kit, 8 rxns K-7220 ExiProgen His-tagged Protein Purification Kit, 16 rxns K-7221 ExiProgen His-tagged Protein Purification Kit, 32 rxns K-7240 ExiProgen Dialysis Kit, 16 rxns www.bioneer.co.kr 15

04 DNA/RNA Preparation ExiPrep 16 Plus 17 ExiCracker NEW 19

Adapt or AC-I nput 100 240V~50/ 60Hz,1. 5A 7A DC-Out put 24VDC,2. I ns t r ument DC-I nput 24VDC,2. 7A

ExiPrep 16 Plus Procedure Experimental Data Figure 1. 전혈에서추출한 genomic DNA 의전기영동분석. 전혈 200 μl 를이용하여 genomic DNA 를추출하고, 100 ng 씩정량하여 1% Agarose gel 에전기영동을수행한결과입니다. Figure 2. 전혈에서추출한 Genomic DNA 의 Real-Time PCR 결과. Alzheimer Disease 와관련된 5 가지의 primer&probe set 을이용하여 Real- Time PCR 을수행한결과입니다. Ordering Information Cat. no. Product Description A-5030 ExiPrep 16 Plus Cat. no. Nucleic Acid Extraction Kits for ExiPrep 16 Plus K-4211 ExiPrep Plus Blood Genomic DNA Kit, 96 rxns K-4214 ExiPrep Plus Bacteria Genomic DNA Kit, 96 rxns K-4215 ExiPrep Plus Plant Genomic DNA Kit, 96 rxns K-4244 ExiPrep Plus Plant Total RNA Kit, 96 rxns K-4271 ExiPrep Plus Viral DNA/ RNA Kit, 96 rxns K-4272 ExiPrep Plus Viral DNA Kit, 96 rxns K-4273 ExiPrep Plus Viral RNA Kit, 96 rxns BIONEER 18

ExiCracker Specifications Dimensions (W x H x D) Weight No. of wells Diameter of wells 300 x 230 x 280 mm 6.5 kg 16 wells 8 mm Description ExiCracker TM 는종자와잎, 뿌리등의식물조직을분쇄할수있는기구입니다. 동시에 16 개시료를분쇄할수있어연구실에서주로사용하는막자와막자사발에비해빠르고간편하며, 시료손실및오염의위험이낮을뿐만아니라당사의자동핵산추출장비인 ExiPrep TM 을이용한자동핵산추출이가능합니다. 또한사용자의편의에따라자사의자성입자기반핵산추출 kit 인 MagListo TM 5M Plant Genomic DNA Extraction Kit 를이용한핵산추출에도사용할수있습니다. Available Experiments with Bioneer s Kits ExiPrep TM Plus Plant Genomic DNA Kit ExiPrep TM Plus Plant Total RNA Kit MagListo TM 5M Plant Genomic DNA Extraction Kit Procedure Features and Benefits 최대 16 개시료를동시에처리 : Sample rack 에는 16 개의시료를넣을수있으며, 기구상단의손잡이를통해공이 (crushing pestle) 를움직여시료를동시에분쇄하므로빠르고간편하게식물시료의전처리가가능합니다. Experimental Data 종자를포함한식물조직으로부터핵산추출 : 호박, 해바라기, 벼와같이크기가크고단단한종자나브로콜리, 양배추등의잎조직분쇄가가능합니다. 반영구적인기구물 : Sample rack 과공이 (crushing pestle) 등의기구물을특수처리한합금소재로제작하여견고할뿐아니라핵산추출용시약에대한부식에강하여반영구적으로사용할수있습니다. Figure 1. ExiCracker TM 와막자사발의성능비교 Ordering Information Cat. no. A-5030-A21 Product Description ExiCracker TM www.bioneer.co.kr 19

05 Electrophoresis Agaro-Power 21

Agaro-Power Description Agaro-Power TM 는 loading adapter 가부착된 agarose gel electrophoresis system 으로써최대 96 개의 sample (size marker 제외 ) 을 2~3 분내로빠르게 loading 할수있도록개발되었습니다. 본제품은폴리카보네이트사출물로제작하여아크릴류의전기영동장치들에비해내구성이뛰어납니다. 또한, power supply 가포함되어있어별도의 power supply 가필요없고간편하게 gel 을 casting 할수있는 gel casting tray 도포함되어있습니다. Features and Benefits Loading adaptor Multi-channel pipette 을이용하여여러 sample 을한번에 loading 하려면 well 이찢어질염려가있고모두정확한위치에 sample 을주입하기가어렵습니다. 바이오니아에서독창적으로개발한 loading adaptor 는정확한 well 의위치를잡아주기때문에더욱빠르고정확하게 loading 할수있습니다. Specifications Distance Between Electrodes Buffer Volume Gel Tray Size (L x W) Comb (No. of teeth) Caster Size (L x W) Input Voltage Output Voltage 228 mm 700 ml 163 x 149 mm 25 well or 34 well/line x 1ea 21 well or 34 well/line x 1ea 15 well or 34 well/line x 1ea 13 well or 34 well/line x 1ea 170 x 156 mm 110 VAC 50/60 Hz, 220VAC 50/60 Hz High:150 V±10% Low : 75 V±10% (User selectable) Power supply Low (75 V) 또는 High voltage (150 V) 를공급하며, ON/OFF system에의해조절이가능합니다. Gel caster & tray Gel caster 를이용하여편리하게 gel 을만들수있습니다. Gel caster 에눈금표시가되어있어동일한두께의 gel 을만들수있습니다. 또한두개의 comb 를사용할경우 gel 분리를쉽게하기위하여 dam 을사용하면편리하게 gel 을만들수있습니다. Comb set Agaro-Power TM 에적용되는 comb 는다양한포맷으로구성되어있습니다. 양쪽이다른형태의 well 로되어있고용도에따라다양하게바꾸어사용할수있습니다. 32 well comb 를모두사용할경우에는 96 개의샘플을한번에 loading 할수있어대용량실험에도편리합니다. (Comb Set 은별도구매이지만, Agaro-Power TM System 구매시 line 당 34/25/21/15/13well 로이루어진 4 개의 Comb Set 이제공됩니다.) Ordering Information Cat. no. Product Description A-7020 Agaro-Power TM System Cat. no. Plastic Consumables A-7020-1 Agaro-Power TM, Power Supply A-7020-2 Agaro-Power TM, Agar Tank/Lid A-7020-3 Agaro-Power TM, Comb Set A-7020-3-1 Agaro-Power TM, Comb (25 well / 34 well) A-7020-3-2 Agaro-Power TM, Comb (13 well / 34 well) A-7020-3-3 Agaro-Power TM, Comb (15 well / 34 well) A-7020-3-4 Agaro-Power TM, Comb (21 well / 34 well) A-7020-4 Agaro-Power TM, Gel Caster/Tray A-7020-5 Agaro-Power TM, Cable Jack www.bioneer.co.kr 21

06 Microbial Culture HT-MegaGrow Shaking Incubator 23

HT-MegaGrow Shaking Incubator Description HT-MegaGrow Shaking Incubator 는표준 96 well plate 를최대 20 개까지장착하여 1,920 종의균주를한꺼번에배양할수있게설계되어 synthetic biology, protein engineering 등의다수의균주들을다루어야하는연구에필수적인장비입니다. 다수의균주들을배양하기위해표준 96 well plate 가일반적으로사용되고있으나, well 의직경이작아혼합이잘되지않는단점이있습니다. 본 incubator 는일반적인 96 well plate 에서혼합이충분히이루어질수있도록회전반경이최적화되어있으며, 빠른회전이가능하도록설계되어, 바이오니아의표준 96 well plate (Cat. no. 90021) 와 deep well 96 plate (Cat. no. 90061) 를사용할경우최소 100 μl 부터 2 ml 까지배양할수있습니다. 특히 HT-MegaGrow Shaking Incubator 는적은양의균주를배양할때수분이증발하는문제를해결하기위하여바이오니아가특수제작한수분공급용기를장착하고있어실험조건을최적화시켜줍니다. Features and Benefits Quick Speed / High Growth Yield: 작은회전반경과빠른회전으로균주를효율적으로배양할수있어균주가성장하는데필요한시간이일반적인 incubator에비해 30 ~ 40% 단축됩니다. Contamination Free: 높은회전수로작동됨에도불구하고 well간의 contamination 가능성이없습니다. High Throughput Sample Growth: 최대 20개의 96 deep well plate를사용하여총 1,920개의시료를동시에배양할수있습니다. Convenience: 배양하기위한균주를 multi-channel pipette 을사용하여각 well 에간편하게접종할수있습니다. 버튼조작이간단하여최소한의조작으로동작이가능합니다. Plate 받침대가상 / 하판으로분리되어있어 plate 의장착과분리가용이합니다. Multiple Functionally & Simple Manipulation: 온도조절기능이있어설정한온도를일정하게유지할수있습니다. 기기작동중온도편차는 ±1 정도로 cell 의 growth condition 이일정하게유지시켜줍니다. 산소양과주입시간이설정된값에의해자동으로조절됩니다. 배양과정에서생기는수분부족현상을막기위해수분공급용기가장착되어있습니다. Specifications <20 개의 plate 를동시장착할수있는내부구조 > Dimension (W x D x H) 725 x 560 x 560 mm Weight (kg) 120 kg Input Voltage 220 Vac, 60 Hz Power Dissipation 450 Watts Operating Temperature 15 ~25 Temperature Range 30 ~40 Temperature Accuracy 설정치 ± 1 Humidity Range over 90% Maximum RPM 1,200 rpm Eccentricity (mm) 4 www.bioneer.co.kr 23

HT-MegaGrow Shaking Incubator Experimental Data Figure 1. Cell Growth Curve. Ordering Information Table.1 Culture Conditions. HT-MegaGrow Plate Media Strain Innoculation Growth Time 37, 1,200 rpm, no aeration, oxygenation (No delay, 1 minute interval, 1 second on) 96 Deep well Plate, round-bottom, square-wells, plate lids(bioneer, Cat. no.90030) 500 μl/well of 1x Terrific Broth _ Ampicillin(100 μg/ml) E. coli, DH5α carrying pbluescript(no insert) 5 μl pre-culture added to each wells with multichannel pipettor 12 hours *standard floor shaker: 37, 750 rpm. Cat. no. Product Description A-4080 HT-MegaGrow Shaking Incubator A-4080-1 96 Shallow Well Plate Rack ( 상 ) A-4080-2 96 Shallow Well Plate Rack ( 하 ) Cat. no. Plastic Consumables 90021 96 Well Plate-RV, 50 ea/pk 90022 96 Well Plate-RV, 100 ea/pk 90065 96 Well Deep Plate for HT-MegeGrow, 10 ea/pk 90066 96 Well Deep Plate for HT-MegeGrow, 20 ea/pk 90067 96 Well Deep Plate for HT-MegeGrow, 50 ea/pk BIONEER 24

07 Vortexing & Spin-down ExiSpin 26

ExiSpin <Presented rotor for 8-tube strip PCR tube [left] and 1.5 μl microtube [right]> Description ExiSpin TM 은실험과정중반복되는 vortex 와 spin-down 과정을기기자체의 program 을이용하여자동으로반복수행할수있는장비입니다. ExiSpin TM 을사용하면 PCR 을비롯한분자생물학연구에있어서실험시간을획기적으로단축할수있습니다. 특히 8-tube strip 4 개를동시에처리할수있어 AccuPower Kit 사용시신속하고재현성이있게반응물을혼합할수있습니다. 분자진단검사분야, 생화학, 분자생물학등 microtube 를사용하는모든분야의연구에서필수적인장비입니다 Features and Benefits Fully Automated Spin-Mix-Spin Technology: Spin-mix-Spin 각단계의시간과속도를설정할수있고반복횟수를 1-999 cycles 입력할수있습니다. 시료의종류에따라다른조건으로 microtube 의용액을 spin down 하고 mixing 하는과정이선택적으로조절가능하며전자동으로작동하여실험의재현성과효율성을높입니다. 1 st spin: Spin down sample Vortexing of samples 2 nd spin: Spin down mixed sample Convenience and High Reproducibility: 시료에따라최적화된실험 protocol을선택하여실험자에따른오차가없이항상동일한조건으로실험할수있습니다. 4개의 8-tube strip을동시에사용할수있어 32개의 PCR/qPCR mixture 를빠르게제조할수있습니다. 또한최대 12개의 1.5 ml 튜브를사용하여세포를파쇄할수있습니다. 입력된프로그램은 poweron 시에계속유지되어단한번의버튼조작으로반복작동이가능합니다. Application Bacterial cell lysis PCR/qPCR 반응 제한효소반응, kinase/ligation 반응 반복적인 mixing과 spin-down이필요한실험 Specifications Dimensions (W x H x D) Weight Spin Regulation Spin Timer Vortexing Strength SMS- Cycle Regulation Power Supply Ordering Information 19 x 23.5 x 12.5 cm 2.7 kg 1,000 ~ 3,500 rpm (increment 100 rpm) 1 sec- 30 min Soft, Medium, Hard 1-999 cycles AC 24 V, 1,250 ma Cat. no. Product Description A-7040 ExiSpin TM Economical and Efficient: ExiSpin TM 은원심분리기능과 vortex 기능을모두갖고있으므로두가지장비를개별적으로구매할필요가없어매우경제적입니다. 또한 microcentrifuge 용 tube 뿐만아니라 PCR tube 사용이가능한 rotor 가추가로제공되어별도의추가액세서리구입을위한비용이절감됩니다. BIONEER 26