Oligo synthesis 4 단계공정을한 cycle 로하여 1 mer 씩 3' 말단에서 5' 말단까지순차적으로합성이진행됩니다. Deblocking Coupling Capping Oxidation OPC Purification Reverse-phase chromato

온라인용 Brochure 입니다. CUSTOM Oligo SYNTHESIS SERVICE

Oligo synthesis 4 단계공정을한 cycle 로하여 1 mer 씩 3' 말단에서 5' 말단까지순차적으로합성이진행됩니다. Deblocking Coupling Capping Oxidation OPC Purification Reverse-phase chromatography resin 이충진된 OPC (Oligonucleotide Purification Cartridge) 컬럼을이용한정제방식으로합성이완료된후불완전한 Oligo( n-1, n-2 mer) 및합성과정에서생성된각종불순물을제거하는정제과정입니다. 모든 Oligo 주문에무료로적용되는기본정제입니다. PAGE Purification 50 mer 이상의긴 Oligo 합성시고순도 oligo 를필요로할때 PAGE 정제를시행합니다. OPC 정제산물을 PAGE (Polyacrylamide Gel Electrophoresis) 로전개하여주밴드만분리하는정제법으로 90% 이상의고순도 Oligo 를확보할수있습니다. 정제과정에서손실이있어최종수득량이다소적을수있습니다. RP-HPLC Purification 50 mer 이하의 Oligo 합성시고순도 Oligo 를필요로할때 RP-HPLC (Reverse-phase HPLC) 정제를시행합니다. RP 컬럼상에서정상적인 Oligo 와불완전한 Oligo 간친유성 (lipophilicity) 차이로분리정제하는방법으로, 90% 이상의고순도 Oligo 를확보할수있습니다. 정제과정상의손실로최종수득량이다소적을수있습니다. Quality Control PAGE Q/C 모든 Oligo는 Polyacrylamide Gel Electrophoresis를통하여품질관리를수행하고있습니다. Size, Multi-band 유무및 Smearing 등의상태를확인하고재합성여부를판단합니다. MALDI-TOF mass spectrometry Q/C MALDI-TOF/MS분석은 50 mer 이하의 Oligo Sample에한하여측정하고있습니다. 이론분자량과측정분자량차및 Multi-peak 유무로재합성여부를판단합니다. Synthesis Scale 0.05 µmole 0.2 µmole 1.0 µmole Available Lengths ~ 90 mer ~ 90 mer ~ 90 mer 가격 ( /mer) 500 1,000 2,000 Synthesis Scale 0.05 µmole 0.2 µmole 1.0 µmole Available Lengths ~ 90 mer ~ 90 mer ~ 90 mer 가격 ( /mer) 300 800 1,800 Synthesis Scale 0.05 umole 0.2 umole 1.0 umole OPC Purification 무료 무료 무료 PAGE Purification 25,000 25,000 35,000 HPLC Purification 30,000 30,000 40,000 택배납품지역 1 차 /2 차접수건 3 차접수 접수 +1 일후발송 발송후 1~2 일이내수령가능 ( 제주및도서, 산간지역제외 ) 1 차 /2 차접수건 3 차접수 홈페이지 ( 월 ~ 금 ) 1 차접수 2 차접수 3 차접수 전일오후 11 시 ~ 당일오전 11 시 50 분 오전 11 시 50 분 ~ 오후 5 시 오후 5 시 ~ 오후 11 시 영업사원배송지역 접수 +1 일후발송 +2 일후발송 서울일부, 경기. 인천, 대전, 부산, 포항, 진주, 대구지역의경우 1 차접수건 - 접수 +1 일후배송 2 차 /3 차접수건 - 접수 +2 일후배송 토요일일요일 / 공휴일 월요일 1 차일괄접수 월요일 1 차일괄접수 or 익일영업일 1 차접수

25 nmole 100 nmole 1 µmole 5 µmole 10 µmole 합성가능길이 (bases) 15 ~ 60 10 ~ 90 5 ~ 100 5 ~ 100 5 ~ 100 5 ~ 100 보장량 3 ODs 6 ODs 15 ODs 45 ODs 225 ODs 450 ODs 가격 ( /base) 530 700 1,400 2,400 13,900 25,000 100 nmole 1 µmole Annealing Service 가격 54,000 72,000 81,000 LabReady Service Fee 가격 LabReady Fee 5,000 RxnReady Mixing Service Fee 가격 25 nm, 100 nm and 250 nm Mixed Oligos 40,000 Normalized Tube Fee 가격 25 nm, 100 nm, 250 nm and 1 um DNA Oligos 10,000 5 µm DNA Oligo 20,000 10 µm DNA Oligo 40,000 Machine Mix Base 무료 Hand Mix Base * 20,000 / 조합

100 nmole (5 ~ 60 bases) (5 ~ 60 bases) 1 µmole (5 ~ 60 bases) 5 µmole (5 ~ 60 bases) 10 µmole (5 ~ 50 bases) RNA bases 10,500/base 13,300/base 28,000/base 77,000/base 160,000/base 2' O-Methyl RNA bases 13,300/base 20,300/base 30,800/base 119,000/base 224,000/base Chimeric DNA bases 700/base 1,400/base 2,400/base 13,900/base 25,000/base RNase-Free HPLC 110,000 150,000 230,000 476,000 826,000 Annealing of Complementary Oligo 54,000 72,000 별도문의별도문의별도문의 * Yields 합성스케일 보장량 (Standard Desalting) 보장량 (RNase-Free HPLC ) 100 nmole 6 ODs 1 OD 15 ODs 3 ODs 1 μmole 45 ODs 15 ODs

PAGE HPLC IE HPLC RNase-Free HPLC Dual HPLC Dual PAGE & HPLC 100 nmole 59,000 54,000 54,000 110,000 112,000-90,000 84,000 84,000 150,000 168,000 182,000 1 µmole 160,000 147,000 147,000 230,000 266,000 336,000 5 µmole 900,000 350,000 350,000 476,000 770,000 1,310,000 10 µmole 1,300,000 470,000 470,000 826,000 1,060,000 2,000,000 100 nmole 1 µmole 5 µmole 10 µmole PAGE Purification 1 OD 2 ODs 10 ODs 50 ODs 100 ODs HPLC Purification 1 OD 4 ODs 20 ODs 100 ODs 200 ODs IE-HPLC Purification 1 OD 4 ODs 20 ODs 100 ODs 200 ODs RNase-Free HPLC Purification 1 OD 4 ODs 20 ODs 100 ODs 200 ODs Dual HPLC Purification 1 OD 2 ODs 10 ODs 50 ODs 100 ODs Dual PAGE & HPLC 0.5 OD 1 OD 5 ODs 25 ODs 50 ODs

Product 정제방법보장량가격 4 nmole Ultramer DNA Oligo (45 ~ 200 bases) Standard Desalt 4 nmole 1,120/base 20 nmole Ultramer DNA Oligo (45 ~ 200 bases) Standard Desalt 20 nmole 2,240/base PAGE-Purified Ultramer DNA Oligo (60 ~ 200 bases) PAGE 정제 Inquire 1,120/base ( 정제비용 130,000 원추가 ) 4 nmole 20 nmole PAGE 200 pmole 4 nmole 20 nmole Description Delivered in Tubes Delivered in Plates 5' Phosphorylation 28,000 35,000 35,000 14,000 14,000 18,000 5' Biotin 56,000 70,000 70,000 28,000 28,000 35,000 5' Amino Modifier C6 34,000 42,000-17,000 17,000 25,000 5' Amino Modifier C12 77,000 98,000-39,000 39,000 49,000 5' deoxyinosine 12,000 14,000 14,000 6,000 6,000 7,000 5' deoxyuridine 12,000 14,000 14,000 6,000 6,000 7,000

4 nmole 20 nmole PAGE 200 pmole 4 nmole 20 nmole Description Delivered in Tubes Delivered in Plates Int deoxyinosine 12,000 14,000 14,000 6,000 6,000 7,000 Int deoxyuridine 12,000 14,000 14,000 6,000 6,000 7,000 Phosphorothioate Bond (PTO) 5,000 5,000 5,000 별도문의 5,000 5,000 2' O-Methyl RNA bases 12,000 19,000 19,000 별도문의 12,000 19,000 3' Ribo A 42,000 56,000 56,000-42,000 56,000 3' Ribo C 42,000 56,000 56,000-42,000 56,000 3' Ribo G 42,000 56,000 56,000-42,000 56,000 3' Ribo U 42,000 56,000 56,000-42,000 56,000 3' Amino Modifier 40,000 49,000 - - 40,000 49,000 5' 5-Methyl dc 70,000 84,000 84,000-70,000 84,000 Int 5-Methyl dc 70,000 84,000 84,000-70,000 84,000 3' Phosphorylation 35,000 42,000 42,000-35,000 42,000 Int Spacer 18 70,000 84,000 84,000-70,000 84,000 3' C3 Spacer 63,000 77,000 77,000 - - - Product 정제방법 보장량 가격 4 nmole Ultramer RNA Oligo (60 ~ 120 bases) Standard Desalt 4 nmole 8,400/ base 20 nmole Ultramer RNA Oligo (60 ~ 120 bases) Standard Desalt 20 nmole 12,600 / base 80 nmole Ultramer RNA Oligo (60 ~ 120 bases) Standard Desalt 80 nmole 22,400 / base Description 4 nmole 20 nmole 80 nmole 5' 6-FAM 91,000 91,000 113,400 5' Amino Modifier C12 77,000 77,000 98,000 5' Amino Modifier C6 36,400 36,400 44,800 5' Biotin 63,000 63,000 77,000 5' deoxyinosine 14,000 14,000 16,800 5' deoxyuridine 14,000 14,000 16,800 5' Phosphorylation 28,000 28,000 35,000

Description (Dye) Positions Ex.(nm) Em.(nm) Alternative Freedom Dyes 5' Internal 3' 6-FAM (NHS Ester) 496 516 6-FAM (Fluorescein) 495 520 Fluorescein dt 495 520 Cy3 550 564 TYE 563 TAMRA 559 583 JOE (NHS Ester) 529 555 MAX (NHS Ester) Cy5 648 668 TYE 665 TAMRA (NHS Ester) 559 583 MAX (NHS Ester) 524 557 JOE (NHS Ester) TET 522 539 Cy5.5 685 706 TYE 705 ROX (NHS Ester) 588 608 ATTO Rho101 (NHS Ester) TYE 563 549 563 Cy3 Yakima Yellow 524 551 HEX 538 555 TEX 615 596 613 Texas Red -X (NHS Ester) TYE 665 645 665 Cy5 TYE 705 686 704 Cy5.5 Alexa Fluor Dyes Alexa Fluor 488 (NHS Ester) 492 517 ATTO 488 (NHS Ester) Alexa Fluor 532 (NHS Ester) 527 553 Alexa Fluor 546 (NHS Ester) 555 571 Alexa Fluor 594 (NHS Ester) 584 616 ATTO 590 (NHS Ester) Alexa Fluor 647 (NHS Ester) 650 670 ATTO 647N (NHS Ester) Alexa Fluor 660 (NHS Ester) 661 691 Alexa Fluor 750 (NHS Ester) 753 775 LI-COR IRDyes 5' IRDye 700 684 702 5' IRDye 800 791 809 5' IRDye 800CW (NHS Ester) 767 791 ATTO Dyes ATTO 488 (NHS Ester) 502 522 Alexa Fluor 488 (NHS Ester) ATTO 532 (NHS Ester) 534 554 ATTO 550 (NHS Ester) 560 575 ATTO 565 (NHS Ester) 570 591 ATTO Rho101 (NHS Ester) 592 609 ROX (NHS Ester) ATTO 590 (NHS Ester) 602 624 Alexa Fluor 594 (NHS Ester) ATTO 633 (NHS Ester) 635 653 ATTO 647N (NHS Ester) 649 662 Alexa Fluor 647 (NHS Ester) Rhodamine Dyes Rhodamine Green -X (NHS Ester) 504 531 Rhodamine Red -X (NHS Ester) 574 594 5-TAMRA (Azide) 546 579 WellRED Dyes WellRED D4 Dye 648 666 WellRED D3 Dye 683 701 WellRED D2 Dye 763 778 Other 6-FAM (Azide) 496 516 Texas Red -X (NHS Ester) 598 617 TEX 615 Lightcycler 640 (NHS Ester) 620 635 Dy 750 (NHS Ester) 747 776

Position 25 nmole 530/mer 100 nmole 830/mer 1,400/mer 1 μmole 2,400/mer 보장량 3 ODs 6 ODs 15 ODs 45 ODs 합성가능길이 15 ~ 60 mer 10 ~ 90 mer 5 ~ 100 mer 5 ~ 100 mer RNA 합성가능 Cholesteryl-TEG 3' - 112,000 112,000 336,000 O O Hexynyl 5' - 112,000 140,000 210,000 O O 5-Octadiynyl du HPLC 정제필수 5' / Internal - 117,600 147,000 220,500 O O 3' - 151,900 186,200 279,300 O O Acrydite 5' - 112,000 140,000 210,000 O Adenylation 5' - 1,120,000 1,393,000 2,450,000 O Amino Modifier 3' - 40,000 49,000 70,000 O Amino Modifier C12 5' 77,000 77,000 98,000 147,000 O Amino Modifier C6 5' 33,600 33,600 42,000 70,000 O Amino Modifier C6 dt Azide (NHS Ester) Biotin Biotin (Azide) Biotin dt Biotin-TEG 5' / Internal - 175,000 210,000 315,000 O 3' - 280,000 280,000 420,000 O internal - 196,000 245,000 371,000 O 5' / 3' - 133,000 168,000 245,000 O O 5' 56,000 56,000 70,000 140,000 O 3' - 84,000 105,000 161,000 O Internal - 137,200 171,500 259,700 O O 5' - 117,600 147,000 220,500 O O 5' / Internal - 245,000 245,000 385,000 O O 3' - 322,000 322,000 511,000 O O 5' - 101,000 126,000 168,000 O O 3' - 84,000 105,000 161,000 O O Digoxigenin (NHS Ester) 5' / 3' - 161,000 161,000 245,000 O O Dithiol Desthiobiotin-TEG 5' / Internal - 245,000 308,000 462,000 O O 3' - 406,000 406,000 616,000 O O 5' / Internal - 168,000 210,000 315,000 O 3' - 553,000 553,000 840,000 O Dual Biotin 5' - 280,000 280,000 420,000 O O I-Linker 1.2 5' - 77,000 105,000 161,000 O PC Biotin 5' - 203,000 203,000 385,000 O Thiol Modifier C6 S-S 5' - 175,000 175,000 273,000 O Thiol Modifier C3 S-S 3' - 140,000 140,000 210,000 O Uni-Link Amino Modifier 5' / Internal - 105,000 133,000 203,000 PAGE 가능 Position 25 nmole 530/mer 100 nmole 830/mer 1,400/mer 1 μmole 2,400/mer 보장량 3 ODs 6 ODs 15 ODs 45 ODs 합성가능길이 15 ~ 60 mer 10 ~ 90 mer 5 ~ 100 mer 5 ~ 100 mer RNA 합성가능 3' Dabcyl 3' - 112,000 140,000 210,000 O O Black Hole Quencher 1 3' - 133,000 175,000 252,000 O Black Hole Quencher 2 3' - 133,000 175,000 252,000 O lowa Black FQ 5' / 3' - 100,800 126,000 189,000 O O Iowa Black RQ 5' / 3' - 100,800 126,000 189,000 O O HPLC 정제필수 PAGE 가능

Position 25 nmole 530/mer 100 nmole 830/mer 1,400/mer 1 μmole 2,400/mer 보장량 3 ODs 6 ODs 15 ODs 45 ODs 합성가능길이 15 ~ 60 mer 10 ~ 90 mer 5 ~ 100 mer 5 ~ 100 mer RNA 합성가능 TAMRA -Sp 3' - 119,000 140,000 210,000 O 5-TAMRA (Azide) 6-FAM (Azide) 6-FAM (Fluorescein) HPLC 정제필수 Internal - 176,400 220,500 333,200 O O 5' - 156,800 196,000 294,000 O O Internal - 224,000 280,000 420,000 O O 5' - 137,200 171,500 254,800 O O 5' - 91,000 113,400 245,000 O 3' - 84,000 105,000 210,000 O Alexa Fluor 488 (NHS Ester) 5' / 3' - 301,000 301,000 455,000 O Alexa Fluor 532 (NHS Ester) 5' / 3' - 413,000 413,000 623,000 O Alexa Fluor 546 (NHS Ester) 5' / 3' - 413,000 413,000 623,000 O Alexa Fluor 594 (NHS Ester) 5' / 3' - 483,000 483,000 735,000 O Alexa Fluor 647 (NHS Ester) 5' / 3' - 301,000 301,000 455,000 O Alexa Fluor 660 (NHS Ester) 5' / 3' - 483,000 483,000 973,000 O Alexa Fluor 750 (NHS Ester) 5' / 3' - 483,000 483,000 735,000 O ATTO 488 (NHS Ester) 5' / 3' - 312,000 416,000 576,000 O ATTO 532 (NHS Ester) 5' / 3' - 312,000 416,000 576,000 O ATTO 550 (NHS Ester) 5' / 3' - 312,000 416,000 576,000 O ATTO 565 (NHS Ester) 5' / 3' - 312,000 416,000 576,000 O ATTO 590 (NHS Ester) 5' / 3' - 312,000 416,000 576,000 O ATTO 633 (NHS Ester) 5' / 3' - 312,000 416,000 576,000 O ATTO 647 (NHS Ester) 5' / 3' - 312,000 416,000 576,000 O ATTO Rho101 (NHS Ester) 5' / 3' - 273,000 364,000 504,000 O Cy3 Cy5.5 Cy5 Internal - 224,000 280,000 420,000 O O 5' - 128,800 161,000 252,000 O O 3' - 133,000 161,000 245,000 O O 5' - 245,000 273,000 413,000 O O 3' - 273,000 315,000 455,000 O O Internal - 224,000 280,000 420,000 O O 5' - 128,800 161,000 252,000 O O 3' - 133,000 161,000 245,000 O O Dy 750 (NHS Ester) 5' - 532,000 532,000 798,000 O 6-FAM (NHS Ester) 5' / 3' - 210,000 266,000 392,000 O Fluorescein dt 5'/ Internal - 245,000 273,000 385,000 O O 3' - 364,000 364,000 511,000 O O HEX 5' - 91,000 113,400 231,000 O IRDye 700 5' - 112,000 140,000 210,000 O IRDye 800 5' - 112,000 140,000 210,000 O IRDye 800CW (NHS Ester) 5' - 350,000 350,000 490,000 O JOE (NHS Ester) 5' / 3' - 273,000 273,000 413,000 O LightCycler 640 (NHS Ester) 5' / 3' - 532,000 532,000 798,000 O MAX (NHS Ester) 5' - 161,000 196,000 294,000 O O 3' - 343,000 343,000 539,000 O O Rhodamine Green -X (NHS Ester) 5' / 3' - 273,000 273,000 413,000 O Rhodamine Red -X (NHS Ester) 5' / 3' - 273,000 273,000 413,000 O PAGE 가능

Position 25 nmole 530/mer 100 nmole 830/mer 1,400/mer 1 μmole 2,400/mer 보장량 3 ODs 6 ODs 15 ODs 45 ODs 합성가능길이 15 ~ 60 mer 10 ~ 90 mer 5 ~ 100 mer 5 ~ 100 mer ROX (NHS Ester) TAMRA (NHS Ester) RNA 합성가능 HPLC 정제필수 5' - 231,000 231,000 350,000 O 3' - - 210,000 315,000 O Internal - 280,000 315,000 553,000 O 5' - 154,000 189,000 301,000 O 3' - 140,000 175,000 273,000 O TET 5' - 91,000 113,400 231,000 O TEX 615 Texas Red -X (NHS Ester) TYE 563 TYE 665 5' - 161,000 196,000 294,000 O O 3' - 189,000 231,000 343,000 O O 5' - 266,000 322,000 462,000 O 3' - 245,000 245,000 413,000 O 5' - 134,400 168,000 252,000 O O 3' - 161,000 196,000 294,000 O O 5' - 134,400 168,000 252,000 O O 3' - 161,000 196,000 294,000 O O TYE 705 5' - 252,000 252,000 385,000 O O WellRED D2 5' - - 273,000 413,000 O WellRED D3 5' - - 273,000 413,000 O WellRED D4 5' - - 273,000 413,000 O Yakima Yellow 5' - 91,000 113,400 231,000 PAGE 가능 Position 25 nmole 530/mer 100 nmole 830/mer 1,400/mer 1 μmole 2,400/mer 보장량 3 ODs 6 ODs 15 ODs 45 ODs 합성가능길이 15 ~ 60 mer 10 ~ 90 mer 5 ~ 100 mer 5 ~ 100 mer Phosphorothioate Bond - 5,000 5,000 7,000 O RNA 합성가능 HPLC 정제필수 PAGE 가능 Position 25 nmole 530/mer 100 nmole 830/mer 1,400/mer 1 μmole 2,400/mer 보장량 3 ODs 6 ODs 15 ODs 45 ODs 합성가능길이 15 ~ 60 mer 10 ~ 90 mer 5 ~ 100 mer 5 ~ 100 mer Phosphorylation RNA 합성가능 HPLC 정제필수 5' 28,000 28,000 35,000 63,000 O O 3' - 35,000 42,000 70,000 O O PAGE 가능 Position 25 nmole 530/mer 100 nmole 830/mer 1,400/mer 1 μmole 2,400/mer 보장량 3 ODs 6 ODs 15 ODs 45 ODs 합성가능길이 15 ~ 60 mer 10 ~ 90 mer 5 ~ 100 mer 5 ~ 100 mer 3' Hexanediol 3' - 50,400 63,000 84,000 O RNA 합성가능 HPLC 정제필수 C3 Spacer 5' / Internal / 3' - 56,000 70,000 105,000 O O dspacer(1,2 -Dideoxyribose) 5' / Internal - 119,000 140,000 210,000 O O 3' - 189,000 189,000 280,000 O O PC Spacer 5' / Internal - 189,000 238,000 357,000 O O Spacer 18 Spacer 9 5' / Internal - 70,000 84,000 126,000 O O 3' - 112,000 112,000 168,000 O O 5' / Internal - 56,000 70,000 105,000 O O 3' - 91,000 91,000 140,000 O O PAGE 가능

Position 25 nmole 530/mer 100 nmole 830/mer 1,400/mer 1 μmole 2,400/mer 보장량 3 ODs 6 ODs 15 ODs 45 ODs 합성가능길이 15 ~ 60 mer 10 ~ 90 mer 5 ~ 100 mer 5 ~ 100 mer 2,6-Diaminopurine (2-Amino-dA) 2-Aminopurine 2'-Fluoro A 2'-Fluoro C 2'-Fluoro G 2'-Fluoro U RNA 합성가능 HPLC 정제필수 5' / Internal - 245,000 245,000 385,000 O O 3' - 322,000 322,000 511,000 O O 5' / Internal - 210,000 210,000 315,000 O 3' - 280,000 280,000 420,000 O 5' / Internal - 42,000 42,000 63,000 O 3' - 56,000 56,000 84,000 O 5' / Internal - 42,000 42,000 63,000 O 3' - 56,000 56,000 84,000 O 5' / Internal - 42,000 42,000 63,000 O 3' - 56,000 56,000 84,000 O 5' / Internal - 42,000 42,000 63,000 O 3' - 56,000 56,000 84,000 O 3' Dideoxy-C 3' - 140,000 175,000 266,000 O O 3' Ribo A 3' - 42,000 56,000 84,000 3' Ribo C 3' - 42,000 56,000 84,000 3' Ribo G 3' - 42,000 56,000 84,000 3' Ribo U 3' - 42,000 56,000 84,000 5-Bromo du 5' / Internal - 112,000 112,000 168,000 O O 5-Hydroxymethyl dc 5' / Internal - 490,000 609,000 910,000 O 3' - 637,000 798,000 1,183,000 O 5-Methyl dc 5' / Internal / 3' - 70,000 84,000 126,000 O O 5-Nitroindole deoxyinosine deoxyuridine Inverted dt 5' / Internal - 210,000 210,000 315,000 O O 3' - 280,000 280,000 420,000 O O 5' / Internal 12,000 12,000 14,000 21,000 O O 3' - 12,000 14,000 21,000 O O 5' / Internal 12,000 12,000 14,000 21,000 O O 3' - 12,000 14,000 21,000 O O 5' - 196,000 245,000 364,000 O 3' - 53,200 63,000 98,000 O O isodc 5' / Internal - 105,000 105,000 210,000 O isodg 5' / Internal - 105,000 105,000 210,000 O Super G 5' / Internal / 3' - 490,000 609,000 910,000 O Super T 5' / Internal / 3' - 203,000 203,000 385,000 O UNA-A 5' / Internal / 3' - 42,000 49,000 70,000 UNA-C 5' / Internal / 3' - 42,000 49,000 70,000 UNA-G 5' / Internal / 3' - 42,000 49,000 70,000 UNA-U 5' / Internal / 3' - 42,000 49,000 70,000 PAGE 가능

Reporter Dye(s) Quencher 합성 Scale / 보장량 100 nmol / 10 nmol 250 nmol / 25 nmol 1 μmol / 50 nmol 5' 6-FAM (Ex.495, Em.520) ZEN / Iowa Black FQ* 270,000 495,000 747,000 5' 6-FAM (Ex.495, Em.520) 5' HEX (Ex.538, Em.555) 5' TET (Ex.522, Em.539) 3' TAMRA 387,000 540,000 828,000 3' Black Hole Quencher -1 300,000 540,000 828,000 3' Black Hole Quencher -2 387,000 540,000 828,000 ZEN / Iowa Black FQ* 300,000 630,000 936,000 3' Black Hole Quencher -1 330,000 684,000 1,035,000 3' Black Hole Quencher -2 477,000 684,000 1,035,000 ZEN / Iowa Black FQ* 441,000 630,000 936,000 3' Black Hole Quencher -1 477,000 684,000 1,035,000 3' Black Hole Quencher -2 477,000 684,000 1,035,000

Reporter Dye(s) Quencher 합성 Scale / 보장량 100 nmol / 2 nmol 250 nmol / 8 nmol 1 μmol / 20 nmol 5' 6-FAM (Ex.495, Em.520) 3' TAMRA NHS Ester - 810,000 1,152,000 5' MAX NHS Ester (Ex.524, Em.557) 5' CY3 (Ex.550, Em.564) 5' TEX 615 (Ex.596, Em.613) 5' CY5 (Ex.648, Em.668) 5' TYE 563 (Ex.549, Em.563) 5' TYE 665 (Ex.642, Em.665) 5' JOE NHS Ester (Ex.529, Em.555) 5' TAMRA NHS Ester (Ex.559, Em.583) 5' ROX NHS Ester (Ex.588, Em.608) 5' Texas Red -X NHS Ester (Ex.598, Em.617) 5' Yakima Yellow (Ex.524, Em.551) ZEN / Iowa Black FQ* 801,000 927,000 1,377,000 3' Black Hole Quencher -1 801,000 927,000 1,377,000 3' Black Hole Quencher -2 801,000 927,000 1,377,000 3' Iowa Black RQ 330,000 468,000 855,000 3' Black Hole Quencher -2 480,000 711,000 1,080,000 3' Iowa Black RQ 567,000 657,000 1,197,000 3' Black Hole Quencher -2 801,000 927,000 1,377,000 3' Iowa Black RQ 330,000 468,000 855,000 3' Black Hole Quencher -2 480,000 711,000 1,080,000 TAO / Iowa Black RQ * 330,000 468,000 855,000 3' Iowa Black RQ 567,000 657,000 1,197,000 3' Black Hole Quencher -2 801,000 927,000 1,377,000 3' Iowa Black RQ 567,000 657,000 1,197,000 3' Black Hole Quencher -2 801,000 927,000 1,377,000 ZEN / Iowa Black FQ * - 630,000 810,000 3' Black Hole Quencher -1-684,000 1,035,000 3' Black Hole Quencher -2-684,000 1,035,000 3' Iowa Black RQ - 621,000 927,000 3' Black Hole Quencher -2-819,000 1,251,000 3' Iowa Black RQ - 621,000 927,000 3' Black Hole Quencher -2-819,000 1,251,000 3' Iowa Black RQ - 621,000 927,000 3' Black Hole Quencher -2-819,000 1,251,000 ZEN / Iowa Black FQ* - 630,000 936,000 5' Reporter Dye(s) Quencher 합성 Scale 보장량가격 5' 6-FAM (Ex.495, Em.520) ZEN / Iowa Black FQ Mini probe 0.5 nmole 100,000 5' Reporter Dye(s) Quencher 합성 Scale 보장량가격 5' 6-FAM (Ex.495, Em.520) ZEN / Iowa Black FQ Eco probe 2.5 nmole 120,000

5' Reporter Dye(s) Quencher 합성 Scale 보장량가격 5' 6-FAM (Ex.495, Em.520) ZEN / Iowa Black FQ* 100nm 5 nmole 498,000 Black Hole Quencher-1 100nm 5 nmole 498,000 Fluorophore * Emission wavelength (nm) 6-FAM 520 TET 539 HEX JOE Yakima Yellow VIC 555 555 549 554 Cy 3 549 ATTO 550 NED 575 575 TAMRA ABY 583 583 ATTO 565 PET 591 595 ROX 608 Texas Red -X JUN ATTO 633 LIZ 617 617 657 655 ATTO 647 669 Cy 5 668

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