03-15(3)-04(고숙주).fm

Transcription

1 Res. Plant Dis. 15(3) : (2009) Research in Plant Disease The Korean Societ of Plant Patholog ZYMV»ƒ e w š *Á½ Á Á½ šá 1 Á 1 û» e, 1 w Effect of the Infection Times b Zucchini ellow mosaic virus on the Yield and Growth in Cucumber Sug-Ju Ko*, Do-Ik Kim, Beom-Rong Kang, Seon-Gon Kim, Yong Hwan Lee 1 and Hong-Soo Choi 1 Environment-Friendl Agricultural Research Instiute, Jeollanamdo Agricultural Research and Extension Services, Naju , Korea 1 Agricultural Microbiolog Division, National Academ of Agricultural Science, Suwon , Korea (Received on September 17, 2009; Accepted on October 21, 2009) We investigated the effect of the infection times and infection degrees at transplanting time of Zucchini ellow mosaic virus (ZYMV) on the growth and ield of cucumber plants at the semi-forcing and the retarding culture in When cucumber was inoculated with ZYMV at transplanting time, 15, 30, 45, 60, and 75 das after transplanting, vine length, internode length, number of leaf of the plants and marketable ield largel decreased as the cucumber infected earlier. The regression models were obtained between the disease incidence levels at 20 das after transplanting as the inoculation degrees and the marketable ield of cucumber: = e x (R 2 = ) at the semi-forcing culture and = 14695e x (R 2 = ) at the retarding culture. And the cucumber ield loss regression models between the smptoms incidence das from the final harvesting time and the rates of ield decrease were expressed as =1.0851x (R 2 = ) at the semi-forcing culture and=1.0439x (R 2 = ) at the retarding culture. Kewords : Cucumber, Infection times, Yield, ZYMV (Cucumis sativus L.) j w. 4,893 ha ƒ 78% wš ( naqs.go.kr). w Cucumber mosaic virus (CMV), Papaa ring spot virus (PRSV), Watermelon mosaic virus 2 (WMV-2), Zucchini ellow mosaic virus (ZYMV) š, m Cucumber green mottle mosaic virus (CGMMV), Kuri green mottle mosaic virus (KGMMV) (Choi, 2001; Ko, 2006; Ullman 1991; Yuki, 2000). potvirus r, ZYMVƒ *Corresponding author Phone) , Fax) ) kosj@korea.kr š (Ko, 2006). ZYMV potvirus w 750 nm ¼ x (Mus persicae) sww 10 w (Lisa Lecoq, 1984; Yuan Ullman, 1996) Lisa Lecoq w j š, ZYMVƒ š (, 1995; ½, 1995). ZYMV n ƒ j w w ù» w, j»x g j vw w. w ZYMV w k vww w ƒ w x w ZYMV»ƒ e w wš w.

2 176 š Á½ Á Á½ šá Á. x û» x ü xs w. xùw x 3 21 x 9 12 w w cm w» x 4 26 l 6 27, x l ¾ ƒƒ w. w 10a (N-P-K)» wš, 20 l 5 10a N, K ƒƒ 1 kg w 1 w.. ZYMV»ƒ e w w» w» w M (ph 7.0) ZYMV š(1:5, w/v) w carborundum(400 mesh) w š w.»», 15, 30, 45, 60, 75 ¾ 15 w. w w» w» x 25%, 50%, 100%, x 10%, 25%, 50%, 100% w. x e ù 3 w, ƒ,,,,, s,,, t w (, 2003). š»» ZYMV.»» 15» 50% û š, 30, 45, 60, 75 60~80%».» 90%.» t û w.»» 6.9%, 23.7%, 48.3% ƒƒ w»¾ ƒq 100%,» 8.5%, 18.3%, 49.1%, 85.0%»¾ ƒw ql (KI7 xg uˆqu iupq iq `YGVu i xg qpg purrq q s t gsq p u s qur iu s q g p q g pu sx q i x qu i i hq s qq t q

3 » ƒƒ 78.1%, 91.7%, 98.7%, 100% ql w ùkû (Fig. 2).»» w ƒ š, ƒ w x» 2ƒ. ƒ û, û» q.» p. ZYMV» p»ƒ w. x» 286 cm, cm, cm, ZYMV»ƒ e w cm, cm, cm, 527 cm (Table 1). x» 227 cm, cm, cm, cm, cm, cm, 444 cm (Table 2).» w w w š, m ƒ. w w. w, s ƒ. w x x w w (Table 1, 2). (KI7 xg uˆqu iupq iq rxqˆqx h u rqi qp u t`ygvg g xg u s uqp u s qur iu s q g p q g pu sx q i x qu i i hq 6CDNG9rrqi r`ygvu rqi u g purrq q s t gsq tqs t ri i hq u qur iu si x q C i xg u uq Vu qxq s t H rxqgˆq C q pqxq s t S qpugq q Fqgrxq s t Fqgr up t T g xg u s uq &$!q \ "!&p $#p $'g hi!&h #85T [ &%q ""p $#p %g $i!!h!85t! '!p ""&p %"i %g "hi " gh "#85T!"%%ip "$!ip %#i %"g "hi!"h $85T!&!"hi "&$hi %'h %"g &hi!$h %#85T "$"h ##h &h %#g "h "!gh Bqgx t xg # %g $#g &%g % g "!g $g It was das after transplanting time. Means followed b a common letter are not significantl different at the 5% b DMRT.

4 178 š Á½ Á Á½ šá Á ZYMV»ƒ w» q. š (2004) CGMMV» w» 35.3%,» 20.9% w š w. x ƒ j» ƒ.»».» ZYMV w e w w x t 12,150 kg/10a w» 2,271 kg 18.7%. 15 4,306 kg 35.5%, 30 5,080 kg 41.9%, 45 5,662 kg 46.6%, 60 9,037 kg 74.4%, 75 9,076 kg 77.2% (Table 3). x» t 455 kg 7,469 kg w 6.1% š, 15 2,365 kg 6CDNG9rrqi r`ygvu rqi u g purrq q s t gsq tqs t ri i hq u q g pu si x q C i xg u uq Vine length H rxqgˆq Internode length Stem diameter Leaf length Leaf width T g xg u s uq %!q \!!#p $'p $i!i %h #85T [ & "p "!i $&p $!ghi!i!h!85t!#"i "! hi & i $"ghi i 'h "#85T!&!"h "#hi &#hi $ hi!i $h $85T!'$&h "#'gh &%ghi $&g "&h "g %#85T " h "#%gh &&gh $%gh #"gh "&g Bqgx t xg """g "'g 'g $#ghi $'g #%g It was das after transplanting time. Means followed b a common letter are not significantl different at the 5% b DMRT. 6CDNG9rrqi r`ygvu rqi u g purrq q s t gsq uqxp ri i hq u qur iu si x q C i xg u uq H r gxr u qg xg H rg wq ghxq H r g wq ghxq Marketable fruit ratio Marketable ield wsg T g xg u s uq $!$ & $! %p \ &% #85T [ ' $% $ ' "!$i!##!85t! %' #!" #&i "' "#85T "# && #&!#% #$$ i "$$ $85T " " #&! '!%h %"" %#85T! "# &% $ # '!%$h %% Bqgx t xg " && #! %& #g It was das after transplanting time. Means followed b a common letter are not significantl different at the 5% b DMRT. Index 6CDNG9rrqi r`ygvu rqi u g purrq q s t gsq uqxp ri i hq u q g pu si x q C i xg u uq No. of total H rg wq ghxq H r g wq ghxq Gg wq ghxqr u g u Gg wq ghxq uqxp wsg T g xg u s uq & '# % "##p \ $ #85T [ '!% ' &#!$#i!%!85t ' #$ $" "$!!#$$i "%& "#85T " # $ "#$!!$"i "# $85T # &# " $& #"' h %!$ %#85T & '!$ %' #'#$h %'& Bqgx t xg!& $! &" %"$'g It was das after transplanting time. Means followed b a common letter are not significantl different at the 5% b DMRT. Index

5 ZYMV»ƒ e w 179 6CDNG9rrqi r`ygv u tu rqi qpxqˆqxg ru tg ˆq u s uq uqxp ri i hq u qur iu si x q [ Infected level g ru tg ˆq uq No. of total H rg wq ghxq H r g wq ghxq Marketable r u g u Marketable uqxpwsg TPC '# # "! $!&i \ #$ TPCC " #' $$ $!%& h! TPCCC $!$ & $! %g &% Bqgx t xg " && #! %& #p Harvesting period : Apr Jun. 27, incidence of ZYMV at the first harvesting time : transplanting time I 15.3%, II 37.3%, III 66.7%. Means followed b a common letter are not significantl different at the 5% b DMRT. Index 6CDNG9rrqi r`ygv u tu rqi qpxqˆqxg ru tg ˆq u s uq uqxp ri i hq u q g pu si x q [ Infected levelg ru tg ˆq uq No. of total No. of marketable No. of g wq ghxq Gg wq ghxqr u g u Gg wq ghxq uqxp wsg TPC % " $!& "#& h \ $" TPCC $ $ "# #&!!''&hi #!$ TPCCC % "" $" "" %&&i!%" TPCV & '# % "##p $ Bqgx t xg!& $! &" %"$'g Harvesting period : Oct Dec. 27, incidence of ZYMV at the first harvesting time : transplanting time I 11.8%, II 25.0%, III 56.0%, IV 96.7%. Means followed b a common letter are not significantl different at the 5% b DMRT. Index (KI7 xg uˆq uqxp rxqˆqx h u rqi qp u t`ygvg g xg u s uqp u s qur iu s q g p q g pu sx q i x q u i i hq

6 180 š Á½ Á Á½ šá Á 31.7%, 30 3,566 kg 47.8%, 45 3,364 kg 45.1%, 60 5,492 kg 73.6%, 75 5,956 kg 79.8% (Table 4).»ƒ š t û, t w.»» w. x» 15.3% 49.4% w š, 37.3% 68.8%, 66.7% 81.3% w (Table 5). x 11.8% 38.6% w š, 25% 46.4%, 56% 62.6%, 96.7% 93.9% w (Table 6).» ùkû, m ƒ. t x 100% w ƒ 0% ƒ¾ w. ù x ƒ w š % w w. ƒ w ù 0% w 100% ùkû (Fig. 2~4). 100% w ƒ 0%ƒ 10, x 15 ƒ,»ƒ x š» š, x» ¼»..» ZYMV 20» w» û w. x = e x (R 2 = ), x = 14695e x (R 2 = ) (Fig. 5). w l» w ¾ mw w. x (KI 7 xg uˆq uqxp `YGVu i xg qpg purrq q s t gsq i i hq r qur iu si x q q g p q g pu s i x qx q

7 ZYMV»ƒ e w 181 (KI Rqxg u tu hq qq u u ugxu iupq iq r`ygvg p uqxp i i hq r qur iu si x q q g p q g pu si x q x q (KIRqxg u tu hq qq pg r ru gxtg ˆq u s uqg p g q r uqxppqi qg qp u t`ygvu rqi u i i hq r qur i u si x q q g p q g pu si x qx q = x (R 2 = ), x = x (R 2 = ) (Fig. 6). mw» dw

8 182 š Á½ Á Á½ šá Á ù s w q. w»» w. 2 w ƒ w».» ƒ s w ƒ» wš w w š» w q. ZYMV»» e w wš w.» l ¾ w»ƒ j w,, ƒ w j w.»ƒ w w.» 20 x = e x (R 2 = ), x = 14695e x (R 2 = ) w. w l» w ¾ mw x = x (R 2 = ), x = x (R 2 = ) w. œ ( : ) w. š x Choi, G. S Occurrence of two tobamovirus diseases in cucurbits and control measures in Korea. Plant Pathol. J. 17: (Cucumis sativus L.) w tobamo- potvirus p w. w ½,,,,, ½, w Zucchini ellow mosaic virus. w ( r) 37: š,, k, Ÿ,, CGMMV»ƒ t e w. 10: Ko, S. J., Lee, Y. H., Cha, K. H., Lee, S. H., Choi, H. S., Choi, Y. S., Lim, G. C. and Kim, K. H Incidence and Distribution of Virus Diseases on Cucumber in Jeonnam Province during Plant Pathol. J. 22: Lisa, V. and Lecoq, H Zucchini ellow mosaic virus. CMI/ AAB Descriptions of plant viruses. No w»». 838 pp. Ullman, D. E., Cho, J. J. and German, T. L Occurrence and distribution of ciucurbit viruses in the Hawaiian Islands. Plant Dis. 75: Yuki, V. A., Reende, J. A. M., Kitajima, E. W., Barroso, P. A. V., Kunuki, H., Groppo, G. A. and Pavan, M. A Occurrence, distribution, and relative incidence of five viruses infecting cucurbits in the state of So Paulo, Brail. Plant Dis. 84: Yuan, C. and Ullman, D. E Comprison of efficienc and propensit as measures of vector importance in Zucchini ellow mosaic potvirus transmission b Aphis gosspii and A. craccivora. Phtopatholog 86: