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샘플발광억제율 샘플함량단위활성혈액샘플희석율표준품 실측샘플발광억제 샘플희석배율 비교활성 단위활성 혈액혈액 - 362 -
억제백분비 대조관측정관대조관 - 363 -
활성 대조관측정관대조관 반응액총량채취반응액량 샘플희석배율 - 364 -
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비효소 샘플군 비효소군 샘플군 비효소군 샘플군단백질 표준농도 - 366 -
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Tble 1. Effect of ethnol on concentrtions of S-contining mino cids nd the metbolites Time 2 h 4 h 6 h 8 h Methionine SAM SAH Cysteine GSH Hypoturine Turine Turine Group nmole/g liver µmole/g liver µmole/ml serum Control 64 ± 2 108 ± 4 25 ± 2 106 ± 4 6.70 ± 0.16 0.20 ± 0.02 2.45 ± 0.43 0.36 ± 0.02 Ethnol 58 ± 2 96 ± 4 24 ± 1 97 ± 3 6.20 ± 0.17 0.20 ± 0.05 3.30 ± 0.52 0.40 ± 0.03 Control 57 ± 6 107 ± 5 24 ± 2 103 ± 2 6.30 ± 0.17 0.16 ± 0.01 3.40 ± 0.33 0.38 ± 0.02 Ethnol 83 ± 5* 110 ± 5 27 ± 3 104 ± 4 4.70 ± 0.13*** 0.46 ± 0.04** 5.06 ± 0.25** 0.85 ± 0.13* Control 59 ± 7 111 ± 4 25 ± 1 117 ± 6 6.18 ± 0.38 0.17 ± 0.01 2.04 ± 0.32 0.33 ± 0.02 Ethnol 79 ± 3* 96 ± 2** 26 ± 2 85 ± 3** 4.04 ± 0.25** 0.46 ± 0.06** 6.92 ± 0.76** 0.92 ± 0.05*** Control 66 ± 3 101 ± 5 22 ± 1 121 ± 8 5.87 ± 0.21 0.19 ± 0.02 2.57 ± 0.61 0.40 ± 0.04 Ethnol 98 ± 12* 85 ± 7* 45 ± 3*** 100 ± 2* 2.96 ± 0.34*** 0.90 ± 0.16** 5.55 ± 0.41** 1.32 ± 0.09*** 18 h Control 111 ± 2 26 ± 2 108 ± 5 6.88 ± 0.21 0.17 ± 0.02 2.43 ± 0.67 N/D Ethnol 96 ± 4** 33 ± 3** 82 ± 3** 5.41 ± 0.21** 0.11 ± 0.01** 2.04 ± 0.58 N/D 24 h 48 h 120 h Control 62 ± 4 108 ± 5 25 ± 2 106 ± 5 6.68 ± 0.19 0.20 ± 0.02 3.25 ± 0.77 0.44 ± 0.05 Ethnol 56 ± 7 92 ± 3* 37 ± 1** 93 ± 7 5.65 ± 0.22* 0.12 ± 0.02* 2.92 ± 0.63 0.41 ± 0.04 Control 64 ± 5 99 ± 4 23 ± 1 108 ± 3 7.19 ± 0.18 0.22 ± 0.02 2.21 ± 0.42 0.40 ± 0.03 Ethnol 72 ± 6 76 ± 4** 23 ± 2 86 ± 6** 6.94 ± 0.76 0.10 ± 0.02** 1.09 ± 0.13* 0.25 ± 0.01** Control 98 ± 4 23 ± 1 113 ± 4 6.64 ± 0.32 0.18 ± 0.02 2.58 ± 0.54 0.29 ± 0.03 N/D Ethnol 99 ± 2 25 ± 1 123 ± 4 7.10 ± 0.12 0.19 ± 0.01 1.01 ± 0.21* 0.25 ± 0.01 168 h Control 107 ± 3 24 ± 1 110 ± 3 7.17 ± 0.14 0.20 ± 0.01 2.28 ± 0.47 N/D Ethnol 105 ± 6 26 ± 1 121 ± 1* 7.56 ± 0.28 0.21 ± 0.05 2.69 ± 0.34 N/D Ech vlue represents the men SEM for more thn four rts. *,**,*** Significntly different from the control t P < 0.05, 0.01, 0.001, respectively (Student's t-test). - 373 -
Time Group MAT C S C L GCS CDO CDC product formed (nmole)/min/mg protein 2 h 4 h 6 h 8 h 24 h 48 h 120 h 168 h Control 0.110 ± 0.002 17.7 ± 0.5 63.6 ± 3.5 7.3 ± 0.3 0.55 ± 0.05 14.9 ± 1.1 Ethnol 0.103 ± 0.006 16.2 ± 0.9 48.9 ±1.5** 6.7 ± 0.2 0.53 ± 0.12 17.2 ± 1.3 Control 0.116 ± 0.006 19.9 ± 0.9 65.0 ± 4.2 7.3 ± 0.5 0.52 ± 0.05 16.0 ± 1.3 Ethnol 0.093 ± 50.7 ± 21.8 ± 1.7 0.003* 2.6* 7.5 ± 0.7 0.70 ± 0.03* 15.2 ± 1.6 Control 0.118 ± 0.007 19.6 ± 0.9 68.8 ± 3.4 7.5 ± 0.4 0.55 ± 0.05 13.4 ± 1.0 0.094 ± 60.6 ± Ethnol 21.5 ± 0.8 7.5 ± 0.3 0.89 ± 0.10* 0.001** 1.3* 17.1 ± 1.1* Control 0.109 ± 0.006 18.9 ± 0.7 72.2 ± 2.8 6.2 ± 0.7 0.49 ± 0.03 15.1 ± 1.0 0.084 ± 15.9 ± Ethnol 67.0 ± 7.7 9.1 ± 1.3* 0.57 ± 0.16 14.3 ± 4.0 0.006* 1.3* Control 0.097 ± 0.004 16.7 ± 0.6 66.4 ± 7.0 6.0 ± 0.2 0.54 ± 0.04 13.1 ± 2.3 8.5 ± 0.34 ± Ethnol 0.103 ± 0.003 17.1 ± 0.5 55.3 ± 2.6 12.9 ± 0.9 0.3** 0.04** 14.9 ± Control 0.103 ± 0.003 16.5 ± 1.7 69.3 ± 5.2 5.7 ± 0.3 0.64 ± 0.04 0.8 10.6 ± 0.25 ± Ethnol 0.113 ± 0.003 16.4 ± 0.4 61.4 ± 4.2 14.1 ± 1.3 0.3*** 0.06** Control 0.121 ± 0.004 16.4 ± 1.2 55.4 ± 3.1 6.8 ± 0.5 0.48 ± 0.02 14.1 ± 0.4 Ethnol 0.120 ± 0.005 18.2 ± 2.1 60.1 ± 2.1 7.2 ± 0.3 0.32 ± 0.04** 15.3 ± 1.1 Control 0.108 ± 0.006 18.3 ± 0.7 58.7 ± 2.2 7.0 ± 0.6 0.53 ± 0.05 15.9 ± 0.9 Ethnol 0.112 ± 0.003 20.3 ±1.4 64.6 ± 7.7 7.2 ± 0.6 0.62 ± 0.05 12.4 ± 2.1-374 -
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4 4 Weeks Ethnol (mg/ml) 3 2 1 12 Weeks 50 Weeks 0 0 50 100 150 200 250 Time (min) 35 700 Triglyceride (mg/g liver) 30 25 20 15 10 5 0 *** ** b Mlondildehyde (nmol/g liver) 600 500 400 300 200 100 0 ** ** b 4 Weeks 12 Weeks 50 Weeks 4 Weeks 12 Weeks 50 Weeks - 377 -
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1000 TOSC of cytosol control DMN 7dys DMN 14 dys DMN 28 dys 800 TOSC / mg protein 600 400 200 b b 0 OH ONOO OOH 80000 TOSC / g liver 60000 40000 20000 b b b b 0 OH ONOO OOH Figure 2. Totl oxyrdicl scvenging cpcity towrds hydroxyl rdicls, peroxynitrite nd peroxyl rdicls of cytosol Rts were given n intrperitonel injection of DMN (10 µl/kg/dy) 3 times per week (3 consecutive dily injections nd 4 dys off per week) for 1, 2, 4 weeks. Animls were scrificed on dys 7, 14 nd 28 from the initition of exposure. Ech vlue represents the men + SEM for more thn 4 rts. Different letters (, b) indicte siginificnt differences between groups (one-wy ANOVA followed by Newmn-Keuls multiple rnge test, P<0.05). - 382 -
1000 TOSC of microsome control DMN 7dys DMN 14 dys DMN 28 dys TOSC / mg protein 800 600 400 200 b b b c 0 OH ONOO OOH 18000 16000 14000 TOSC / g liver 12000 10000 8000 6000 4000 b b b c b b b 2000 0 OH ONOO OOH Figure 3. Totl oxyrdicl scvenging cpcity towrds hydroxyl rdicls, peroxynitrite nd peroxyl rdicls of heptic microsome Rts were given n intrperitonel injection of DMN (10 µl/kg/dy) 3 times per week (3 consecutive dily injections nd 4 dys off per week) for 1, 2, 4 weeks. Animls were scrificed on dys 7, 14 nd 28 from the initition of exposure. Ech vlue represents the men + SEM for more thn 4 rts. Different letters (, b, c) indicte siginificnt differences between groups (one-wy ANOVA followed by Newmn-Keuls multiple rnge test, P<0.05). - 383 -
TOSC of serum control DMN 7 dys DMN 14 dys DMN 28 dys 250 200 TOSC / mg protein 150 100 50 0 OH ONOO OOH TOSC / ml 12000 10000 8000 6000 4000 b b b b b b b 2000 0 OH ONOO OOH Figure 5. Totl oxyrdicl scvenging cpcity towrds hydroxyl rdicls, peroxynitrite nd peroxyl rdicls of serum Rts were given n intrperitonel injection of DMN (10 ml/kg/dy) 3 times per week (3 consecutive dily injections nd 4 dys off per week) for 1, 2, 4 weeks. Treted nimls were scrificed on dys 7, 14 nd 28 from the initition of exposure. Ech vlue represents the men + SEM for more thn 4 rts. Different letters (, b) indicte siginificnt differences between groups (one-wy ANOVA followed by Newmn-Keuls multiple rnge test, P<0.05). - 384 -
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