ISSN 1599-8630 Vol.8 No.2 Korea Biosafety Clearing House Vol.8 No.2 (2007 7) >> >> >> >> >> >> >>
Contents 2007. 07 Biosafety Vol.8, No.2 BIOSAFETY
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형질전환 조류를 이용한 바이오신약 생산기술 한재용 교수는 서울대학교 동물자원학과 및 동 대학 대학원을 종업, 미국 미네소타 주 한 재 용 교수 립대학교에서 동물분자유전학으로 박사학위를 받았다. 2002년부터 2005년까지 농 촌진흥청 바이오그린21사업 가금연구단장을 역임한 바 있으며, 현재는 모교인 서울대 서울대학교 농생명공학부 jaehan@snu.ac.kr 22 BIOSAFETY Vol.8 No.2 학교에서 농생명공학부 교수로서, 한국가금학회 부회장, 아시아태평양 축산학회 편집 위원직을 맡고 있다.
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기획 형질전환 조류를 이용한 바이오신약 생산기술 Lillico SG, Sherman A, McGrew MJ, Robertson CD, Smith J, Haslam C, Barnard P, Radcliffe PA, Mitrophanous KA, Elliot EA, Sang HM. Oviduct-specific expression of two therapeutic proteins in transgenic hens. Proc Natl Acad Sci USA 2007;104:1771-1776 Love J, Gribbin C, Mather C, Sang H. Transgenic birds by DNA microinjection. Biotechnology(NY) 1994;12:60-63 McGrew MJ, Sherman A, Ellard FM, Lillico SG, Gilhooley HJ, Kingsman AJ, Mitrophanous KA, Sang H. Efficient production of germline transgenic chickens using lentiviral vectors. EMBO Rep 2004;5:728-733 Mizuarai S, Ono K, Yamaguchi K, Nishijima K, Kamihira M, Iijima S. Production of transgenic quails with high frequency of germ-line transmission using VSV-G pseudotyped retroviral vector. Biochem Biophys Res Commun 2001;286:456-463 Mozdziak PE, Borwornpinyo S, McCoy DW, Petitte JN. Development of transgenic chickens expressing bacterial beta-galactosidase. Dev Dyn 2003;226:439-445 Naito M, Sasaki E, Ohtaki M, Sakurai M. Introduction of exogenous DNA into somatic and germ cells of chickens by microinjection into the germinal disc of fertilized ova. Mol Reprod Dev 1994;37:167-171 Pain B, Clark ME, Shen M, Nakazawa H, Sakurai M, Samarut J, Etches RJ. Long-term in vitro culture and characterisation of avian embryonic stem cells with multiple morphogenetic potentialities. Development 1996;122:2339-2348 Park HJ, Park TS, Kim TM, Kim JN, Shin SS, Lim JM, Han JY. Establishment of an in vitro culture system for chicken preblastodermal cells. Mol Reprod Dev 2006;73:452-461 Park TS, Hong YH, Kwon SC, Lim JM, Han JY. Birth of germline chimeras by transfer of chicken embryonic germ (EG) cells into recipient embryos. Mol Reprod Dev 2003;65:389-395 Patel TB, Pequignot E, Parker SH, Leavitt MC, Greenberg HE, Kraft WK. Transgenic avian-derived recombinant human interferon-alpha2b (AVI-005) in healthy subjects: an open-label, single-dose, controlled study. Int J Clin Pharmacol Ther 2007;45:161-168 Rapp JC, Harvey AJ, Speksnijder GL, Hu W, Ivarie R. Biologically active human interferon alpha-2b produced in the egg white of transgenic hens. Transgenic Res 2003;12:569-575 Salter DW, Smith EJ, Hughes SH, Wright SE, Crittenden LB. Transgenic chickens: insertion of retroviral gene into chicken germ line. Virology 1987;157:236-240 Scott BB, Lois C. Generation of tissue-specific transgenic birds with lentiviral vectors. Proc Natl Acad Sci USA 2005;102:16443-16447 van de Lavoir MC, Diamond JH, Leighton PA, Mather-Love C, Heyer BS, Bradshaw R, Kerchner A, Hooi LT, Gessaro TM, Swanberg SE, Delany ME, Etches RJ. Germline transmission of genetically modified primordial germ cells. Nature 2006;441:766-769 Zhu L, van de Lavoir MC, Albanese J, Beenhouwer DO, Cardarelli PM, Cuison S, Deng DF, Deshpande S, Diamond JH, Green L, Halk EL, Heyer BS, Kay RM, Kerchner A, Leighton PA, Mather CM, Morrison SL, Nikolov ZL, Passmore DB, Pradas-Monne A, Preston BT, Rangan VS, Shi M, Srinivasan M, White SG, Winters-Digiacinto P, Wong S, Zhou W, Etches RJ. Production of human monoclonal antibody in eggs of chimeric chickens. Nat Biotech 2005;23:1159-1169 Park TS, Han JY. Derivation and characterization of pluripotent embryonic germ cells in chicken. Mol Reprod Dev 2000;56:475-482 KBCH 31
형질전환과 체세포 복제기술을 이용한 바이오장기 생산 이훈택 교수는 건국대학교 축산학과 및 동 대학 대학원을 졸업, 미국 일리노이주립대학 이 훈 택 교수 교에서 분자내분비학으로 박사학위를 받았다. 농림부의 차세대 성장동력사업인 바이오 장기(무균돼지) 연구과제(2004년~2006년)의 총괄책임자를 맡은 바 있으며, 현재는 건국대학교 동물생명과학대학 htl3675@konkuk.ac.kr 32 BIOSAFETY Vol.8 No.2 건국대학교 동물생명과학대학 교수로서, 건국대학교 의생명과연구원 바이오장기센터 소장, 한국동물번식학회 부회장 및 아시아동물생명공학회 부회장직을 역임하고 있다.
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기획 형질전환과 체세포 복제기술을 이용한 바이오장기 생산 Osborne FN. Am J Transplant 2005;5:1248-55 Rood PPM et al. Am J Transplantation 2006;6:1269-1274 Ramirez P et al Transplantation Proceed 2005;37:4103-4106 Reemtsma K et al. Ann Surg 1964;160:384-412 Salomon DR et al, Nature 2000;407:90-94 Schlitt H et al. Langenbecks Arch Surg 1999 Schuurman HJ et al. Transplantation 2002;73:1146-51 Scobie L et al, Transplantation Society Congress, Rome 2000 Taylor LA, Greenstein SM2007, http://www.emedicine.com/med/topic3715.htm Vanderpool HC et al. J Law Med Ethics 1999;27:153-184 Waterworth PD et al. J Heart Lung Transplant 1998;17:1201-136 Wilmut I et al. Nature 1997;385:810-816 Wilson CA et al. J Virol 1998;72:3082-3087 Wu GS et al. Xenotransplantation 2005;12:197-208 Yau-Lin Tseng et al. Transplantation 2005;80:1493-1500 Yoichi Takahagi et al. Molecular Repoduction and Development 2005;71:331-338 Zaidi A et al. Transplantation 1998;65:1584-1594 Zawada WM et al. Nature Med 1998;4:569-572 OECD 2005, http://www.oecd.org/document/3/0,2340,en_2649_33703_34962243_1_1_1 _1,00.html OECD/WHO, 2001 이종간 장기이식에 관한 감독규정 http://whqlibdoc.who.int/hq/2001/who_cds_csr_eph_2001.1.pdf WHO 규정, http://whqlibdoc.who.int/hq/2001/who_cds_csr_eph_2001.2.pdf WHO 제네바협약, 2005, http://www.who.int/transplantation/xenoenglish.pdf BUSINESS COMMUNICATIONS COMPANY, 2004. Biotechnology Industry Review. http://www.bccresearch.com/bio/bio001e.asp 미국 FDA 보고서 2006, http://www.fda.gov/cber/xap/xap.htm 호주보고서 2005 http://www.nhmrc.gov.au/about/committees/expert/gtrap/_files/xenotrans.pdf KBCH 45
형질전환 동물 및 동물복제 관련 국내 특허 동향 이 충 호 사무관 이충호 사무관은 서울대학교 수의학과를 졸업하고 동 대학 대학원에서 수의학으로 박사 특허청 식품생물자원심사팀 vetho@kipo.go.kr 46 BIOSAFETY Vol.8 No.2 학위를 받았다. 특허청 유전공학심사팀 및 생명공학심사팀에서 형질전환 및 체세포 복제 기술에 관한 심사를 4년 가까이 하였으며, 현재는 특허청 식품생물자원팀에 소속되어 있다.
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동물세포를 이용한 치료용 단백질 생산기술 나 규 흠 수석연구원 나규흠 박사는 연세대학교 생명공학과를 졸업, 동 대학 대학원에서 미생물공학으로 동아제약(주) 연구소 바이오텍 연구원 khna@donga.co.kr 56 BIOSAFETY Vol.8 No.2 석사를 마친 후 성균관대학교에서 제조약학으로 박사학위를 받았다. 1989년부터 현 재까지 동아제약(주) 연구소 바이오텍 연구원에서 수석연구원으로 재직중에 있다.
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기획 동물세포를 이용한 치료용 단백질 생산기술 참고문헌 Kwaks, T.H.J. and Otte, A.P. Employing epigenetics to augment the expression of therapeutic proteins in mammalian cells. Trends in Biotechnology 24, 137-142 (2006) Le Hir, H., Nott, A. and Moore, M.J. How introns influence and enhance eukaryotic gene expression. Trends in Biochemical Science 28, 215-220 (2003) Markrides, S.C. Components of vectors for gene transfer and expression in mammalian cells. Protein Expression and Purification 17, 183-202 (1999) Anderson, D.C. and Krummen, L. Recombinant protein expression for therapeutic applications. Current Opinion in Biotechnology 13, 117-123 (2002) Arden, N., Nivtchanyong, T. and Betenbaugh, M.J. Cell engineering blocks stress and improves biotherapeutic production. Bioprocessing 3, 23-28 (2004) Barnes, L.M., Bentley, C.M. and Dickson, A.J. Characterization of the stability of recombinant protein production in the GS-NS0 expression system. Biotechnology and Bioengineering 73, 261-270 (2001) Butler, M. Animal cell culture: recent achievements and perspectives in the production of biopharmaceuticals. Applied Microbiology and Biotechnology 68, 283-291 (2005) Chadd, H.E. and Chamow, S.M. Therapeutic antibody expression technology. Current Opinion in Biotechnology 12, 188-194 (2001) Chu, L. and Robinson, D.K. Industrial choices for protein production by large-scale cell culture. Current Opinion in Biotechnology 12, 180-187 (2001) Hesse, F. and Wagner, R. Developments and improvements in the manufacturing of human therapeutics with mammalian cell cultures. Trends in Biotechnology 18, 173-180 (2000) Hu, W-S and Aunins, J.G. Large-scale mammlian cell culture. Current Opinion in Biotechnology 8, 148-153 (1997) Nevalainen, K.M.H., Te o, V.S.J. and Bergquist, P.L. Heterologous protein expression in filamentous fungi. Trends in Biotechnology 23, 468-474 (2005) Sethuraman, N. and Stadheim, T.A. Challenges in therapeutic glycoprotein production. Current Opinion in Biotechnology 17, 341-346 (2006) Sinacore, M.S., Drapeau, D. and Adamson, S.R. Adaptation of mammalian cells to growth in serumfree media. Molecular Biotechnology 15, 249-257 (2000) Wurm, F.M. Production of recombinant protein therapeutics in cultivated mammalian cells. Nature Biotechnology 22, 1393-1398 (2004) Wurm, F.M. and Pallavicini, M.G. and Arathoon, R. Integration and stability of CHO amplicons containing plasmid sequences. Developments in Biological Standardization 76, 69-82 (1992) Zeng, H. and Willson, J.H. Gene targeting in normal and amplified cell lines. Nature 344, 170-173 (1990) Recombinant therapeutic proteins, Datamonitor DMHC1975 (2004. 4.) Therapeutic proteins, Datamonitor DMHC1803 (2002. 8) 박홍우, CHO 세포 고농도배양을 위한 핵심기반 기술개발. 산업자원부 산업기술개발사업 기술개발보고서 (2005. 8.). 나규흠, 재조합 동물세포주의 세포기질 평가 및 특성분석, 생명공학의약품안전평가기반기술구축연구보고서, 1, 177196 (2003) KBCH 69
형질전환 동물을 이용한 바이오의약품 개발의 기업 연구개발 현황 김 훈 택 수석연구원 김훈택 연구원은 1990년부터 2000년까지 10년간 SK케미칼에서 근무하였고, 현재는 (주)인투젠 연구개발실 htkim@in2gen.com 70 BIOSAFETY Vol.8 No.2 SK케미컬이 1998년에 인수한 바이오 벤처기업인 (주)인투젠의 연구개발실에서 수 석연구원으로 근무하고 있다.
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