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1 理 蓮 臨 類 V CNPH 行 93 年 年 參 行 理 94 年 2 21

2 濫 不 諸 類 料 (1) 歷年來 (70-92 年 ) 六 更 年來 (80-92 年 ) 療 金 蓮 葉 龍 蓮 蓮 療 (2) 蓮 葉 Scutellria barbata Don 臨 (3, 4) 蓮 D- 糖 (D-galactosamine) (Acetaminophen) 不 (5) 蓮 Chou (6) (7-12) 蓮 Baicalein Baicalin Wogonin 蓮 理 Kimuya (13) Abe (14) (15-19) Baicalein Baicalin Wogonin apigenin luteolin 類 列 殺 (20) 茶 protocatechuic acid 類 (21) 蓮不 療 療 臨 見 都 量 便 1976 年 (22) 量 3.5 % 49.8 % 46.7% (23-27) (28) (29) 臨 流 (23-26,,28, 30-31) 臨 流 行 綠 臨 流 良 離 臨 度 (304.2 K) 臨 力 ( psi) 行 别 離 不 便 率 易 離 臨 蓮 naringenin luteolin protocatechuic acid wogonin baicalein baicalin apigenin 量 益 理

3 1. 蓮 粒 量 105 量 蓮 量 12.1 % 量 (dry basis) 2. 臨 (SFE) 臨 流 ISCO 臨 流 良 量 力 40Mpa 數 (Fig. 1) 量 蓮 (cartridge) 度 力 行 HPLC 度 3. Naringenin Aldrich HPLC 度 HPLC JASCO UV/Vis (photodiode) 離 Inertsil 5C 18 ODS mm 度 250 mm 度 45 流 流 1 ml/min protocatechuic acid 流 0.1% H 3 PO nm naringenin luteolin baicalin apigenin 流 0.1% H 3 PO 4 -CH 3 CN (80:20, v/v) 280 nm baicalein 流 0.1% H 3 PO 4 -CH 3 CN (75:25, v/v) 280 nm wogonin 流 0.1% H 3 PO 4 -CH 3 CN (70:30, v/v) 280 nm 論 1. HPLC 精 量 0.5 mg/ml Naringenin 六 (intraday) 連 六 (interday) 兩 HPLC 精 wogonin baicalein baicalin naringenin luteolin apigenin protocatechuic acid intraday (RSD) wogonin baicalein baicalin naringenin luteolin apigenin protocatechuic acid interday (RSD) 兩 (RSD) 略 4% 精 2. 臨 (1) 臨

4 臨 力 bar 度 K 4 諸 wogonin CO 2 (2) 臨 量 力 171 bar 度 325K 量 量 2 度 2.13 % 量 wogonin 量 量 論 Lucien Foster 契 11.5% 度 力 量 3 力 171 bar 度 K wogonin 量 度 度 臨 度 降 K 量 臨 度 4 度 325K wogonin 量 力 力 臨 度 度 Hildebrand 3 4 wogonin 力 bar 度 K 臨 wogonin 力 度 論 臨 蓮 臨 wogonin (CNPH 93-10) 參 1. ( 料 2. Chiu, H. F., Lin, C. C., Yang, C. C. and Yang, F., The Pharmacological and Pathological Studies on Several Hepatic Protecttive Crude Drugs from Taiwan(I), American Journal of Chinese Medicine, 16(3-4), , Chiu, F. H., Lin, C. C., Yen, M. H., Wu, P. S. and Yang, C. Y., The Pharmacological and Pathological Studies on Several Hepatic Protective Crude Drugs from Taiwan (V): The Effects of Bombax Malabrica and Scutellaria Rivularis, Am. J. Chin. Med., 4, , Lin, S. C., Lin, C. C., Lin, Y. H. and Chen, C. H., Protective and Therapeutic Effects of Ban-Zhi-lian on Hepatotoxin-induced Liver Injuries, Am. J. Chin. Med., 22, 29-42, 林 蓮 180 論 73 年度 6. Chou, C. J., A New Favone from Scutellaria Rivularis, J. Taiwan Parm. Assoc., 30, 36-43, Miyaichi, Y. and Tomimori, T., Studies on the Constituents of Scutellaria Species XVI, On

5 the Phenol Glycosides of the Root of Scutellaria Baicalensis Georgi, Natural Med., 48, , Chou, C. J. and Lin, S. Y., Studies on the Constituents of Scutellaria Rivularis Roots( ), J. Taiwan Parm. Assoc., 37, , Tomimori, T., Miyaichi Y., Imoto, Y. and Kizu, H., Studies on the Constituents of Scutellaria Species X, On the Flavonoid Constituents of Ban Zhi Lian the Whole Herb of Scutellaria Rivalaris Wall(I), Shoyakugaku Zasshi, 38, , Tomimori, T., Lin, H., Miyaichi, Y., Toyofuku, S. and Namba, T., Studies on the Constituents of Scutellaria Species VI, On the Flavonoid Constituents of the Root of Scutellaria Baicalensis Georgi (5), Quantitative analysis of Flavonoids in Scutellaria Roots by High-performance Liquid Chromatography, Yakugaku Zasshi, 105, , Tomimori, T., Miyaichi, Y., Imoto, Y. and Kizu, H., Studies on the Constituents of Scutellaria Species VII, On the Flavonoid Constituents of Ban Zhi Lian, the Whole Herb of Scutellaria Rivalaris Wall(2), Shoyakugaku Zasshi, 40, , Tomimori, T., Imoto, Y. and Miyaichi, Y., Studies on the Constituents of Scutellaria Species XII, On the Flavonoid Constituents of Root of Scutellaria Rivularis Wall, Chem. Pharm. Bull., 38(12), , Kimuya, Y., Kubo, M., Tani, T., Arichi, S. and Okuda, H., Studies on Scutellaria Radix IV, Effects on Lipid Peroxidation in Rat Liver, Chem. Pharm. Bull., 29, , Abe, K., Inoue, O. and Yumioka, E., Binary Excretion of Metabolites of Baicalin and Baicalein in Rats, Chem. Pharm. Bull., 38(1), , Lin, C. C. and Shieh, D. E., The Anti-inflammatory Activity of Scutellaria Rivalaris Extractions and Its Active Components, Baicalin, Baicalein and Wogonin, The 10 th Symposium on Natural Products, Kaohsiung, Taiwan, R.O.C., pp , Lin, C. C. and Shieh, D. E., In Vivo Hepatoprtective Effect of Baicalin, Baicalein and Wogonin from Scutellaria Rivalaris, The Bull. Of Society of Pharmacognosy, R.O.C., NO. IV, pp , 呂 : Baicalein 類 :Shikonin 類 論 89 年度 18. 類 Hep G2 cells 滑 理 論 82 年度 19. 呂 (1) (2) Baicalein 論 89 年度 20. Kobayashi, T., Nakata, T. and Kuzumaki, T., Effect of Favonoids on Cell Cycle Progression in Prostate Cancer Cells, Cancer Lett., Feb 8, 176 (1), 17-23, Hudson, E. A., Dinh, P. A., Kokubun, T., Simmonds, M. S. and Gescher, A., Characterization of Potentially Chemopreventive Phenols in Extracts of Brown rice that Inhibit the Growth of Human Colon Cancer cells, Cancer Epidemiol Biomarkers Prev., Nov, 9(11), , 年度 Baysal, T. and Starmans, D. A. J., Supercritical carbon dioxide extraction of carvone and limonene from caraway seed, Journal of Supercritical Fluids, 14, , Yepez, B., Espinosa, M., Lopez, S. and Bolanos, G., Producing antioxidant fractions from herbaceous matrices by supercritical fluid extraction, Fluid Phase Equilibria, , , Lucas, A., Martinez, E., Rincon, J., Balanco, M. A. and Gracia, I., Supercritical fluid extraction of tocopherol concentrates from olive tree leaves, Journal of Supercritical

6 Fluids, 22, , Chiu, K. L., Cheng, Y. C., Chen, J. H. Chang, C. J. and Yang, P. W., Supercritical fluids extraction of Ginkgo ginkgolides and flavonids, Journal of Supercritical Fluids, 24, 77-87, Chen, J. H., Xia, Z. H. and Tan, R. X., High-performance liquid chromatographic analysis of bioactive triterpenes in Perilla frutescens, Journal of Pharmaceutical and Biomedical Analysis, 32, , Lin, M. C., Tsai, M. J.and Wen, K. C., Supercritical fluid extraction of flavonids from Scutellariae Radix, Journal of Chromatography A, 830, , Sha, Y. F., Huang, Tao M., Shen, S. and Duan, G. L., Determination of volatile compounds in Magnolia Bark by microwave-assisted extraction couple to headspace solid-phase microextraction and gas chromatography-mass spectrometry, The Japan Society for Analytical Chemistry, 20, , Catchpole, O. J., Perry, N. B., da Silva, B. M. T., Grey, J. B. and Smallfied B. M., Supercritical extraction of herbs Ι: Saw Palmetto, St John, s Wort, Kava Root, and Echinacea,, Journal of Supercritical Fluids, 22, , Zancan, K. C., Marques, M. O.M., Petenate, A. J., Meireles, M. A. A., Extraction of ginger (Zingiber officinale Roscoe) oleoresin with carbon dioxide and co-solvents: a study of the antioxidant action of the extracts, Journal of Supercritical Fluids, 24, 57-76, L u q u e d e C a s t r o, M. D. and Te n a, M. T., S t ategies for Supercritical Fluid Extraction of Polar and Ionic Compounds, Trends in Analytical Chemistry, 15, 32-37, Fig. 1. A schematic diagram of the extraction apparatus of supercritical carbon dioxide.

7 2.0 Conents of components (mg/g) :Baicalin :Baicalein :Wogonin Percentage of alcohol (%) Fig. 2. Effect of the amount of co-solvent added on the extraction of three constituents from Scutellaria Barbata Don as function of percentage of alcohol (SCE at 171 bar and 328K). 2.4 Conents of components (mg/g) :Baicalin :Baicalein :Wogonin Temperature (K) Fig. 3. Effect of temperature on the extraction of three constituents from Scutellaria Barbata Don (SCE at 171 bar and 11.5% alcohol).

8 2.4 Contents of components (mg/g) :Baicalin :Baicalein :Wogonin Pressure (bar) Fig. 4. Effect of pressure on the extraction of three constituents from Scutellaria Barbata Don (SCE at 328 K and 11.5% alcohol).