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1 CNIC 行 94 年 年 年

2 老 行 不 利 酪 力 DPPH TEAC 利 MPA580 料 行 類 參 年 量 利 領 歷 理 來 易 度 易 理 來 更 力 了 歷 數 更 年 更 爲 便 更 2

3 老 留 識 流 列 流 識 來 來 陸 Hu, G. (Chrysanthemum ramat) (Crocus sativus L) 精 (2-hydroxy-4-methoxybenzophenone-5-sulfonic acid) 力 不 力 論 令 例 蘆 金 金縷 例 料 蘆 A. barbadensis MilleAloe vera extract 金 Calendula officinal Calendula extract 刺 狀 L. Matricaria chamomilla. L. Chamomile fluid extract Zingiber officinale Ginger root extract 金縷 Hamamelis virginiana L. Hamaelis extract Rosmarinus Rosemary extract or oil 3

4 officinalis L. 茶 Melaleuca alternifolia Ch. Tea tree oil 狀 Triticum sp. Wheat germ oil 1. 力 DPPH (1,1-diphenyl-2-picryl hydrazyl) 375ul sample 20mg/l DPPH 750ul nm ABTS free radical 1.5ml 離 ABTS(1000uM) 0.25ml H 2 O 2 (500uM)0.25ml Peroxidase (44units/ml)0.25ml ml nm Liposome system 1cc Liposome(10mg/1cc NaH 2 PO 4 -Na 2 HPO 4 ) 1cc FeCl 3 (400uM) 1cc 1cc Ascorbic Acid(400uM) cc TBHQ(10mg/1cc ) 2cc TCA-TBA-HCL

5 2000rpm 離 nm 2. 酪 量 精 70 units mushroom tyrosinase 0.5 ml L-tyrosine (0.1 mg/ml) 利 度 量 475 nm 100 酪 酪 3. 2 ml 0.01 % (0.123 mg/ 5 ml 12.5 g methylene chloride, 37.5 g cyclohexane, 50 g isopropanol) 250~400 nm 行 數 5

6 (2 mg/cm 2 ) (Transpore TM tape) In vitro SPF ( UV1000S) 量 280~400 nm 率 率 SPF 4. 老 不 度 extracts 3 I III 行 力 老 力 例 丹 30 (Glycyrrhiza glabra) (twig of Morus alba) (root-bark of Morus alba) 丹 (Paeonia suffruticosa ANDREWS) (Chaenomeles speciosa) 酪 力 30 (Glycyrrhiza glabra L.) (Citrus reticulate Blanco) (Scutellaria baricalensis GEORGI) 金 (Lonicera japonica Thunb) 連 (Coptis chinensis Franch) 丹參 (Salvia 6

7 miltiorrhiza Bge) (Phellodendron chinense schneid) 力 連 老 力 論 流 ; 來 更 流 力 列 療 六 參 1. Bernard, P. and Berthon, J. Y Resveratrol: an original mechanism on tyrosinase inhibition. International journal of Cosmetic Science. 22, Boots the chemist Ltd. The guide to practical measurement of UVA/UVB ratios. The Boots Chemist, PLC, Nottingham, England. 3. Cabanes, J. et al Kojic acid, a cosmetic skin whitening agent, is a slow-binding inhibitor of catecholase activity of tyrosinase. J. Pharm. Pharmacol. 46, Cosmetics & toiletries Natural and Botanical products formulary. Vol 111, Easton, A. Women have deadly desire for paler skin in the 7

8 Philippines. The Lancet 352, Fitzpartrick, T. B Pathophysiology of hypermelanoses. Clin. Drug. Invest. 10 (suppl. 2) 7. Goihman-Yahr, M Skin aging and photoaging: an outlook. Clinics in Dermatology. 14, Lee, K. T. et al., Biological screening of 100 plant extracts for cosmetic use (I): inhibitory activities of tyrosinase and DOPA auto-oxidation. International Journal of Cosmetic Science. 19, Lin, C.-G., Kao, Y.-T., Liu, W.-T., Huang, H.-H., Chen, K.-C. and Lin, H.-C Cytotoxic effects of Bacillus anthracis lethal toxin on macrophage-like cell line. Current Microbiology 33, Luckewicz, W Determination of ascorbly dipalmitate in cosmetic whitening powders by differential scanning calorimetry. J. Soc. Cosmet. Chem Maeda K. et al In vitro effectiveness of several whitening cosmetic components in human melanocytes. J. Soc. Cosm. Chem. 42, Masuda, M. et al Skin lighteners. Cosmet. Toil. 111, Melo, P. S., Duran, N., and Haun, M. Cytotoxicity of prodigisin and benznidazole on V79 cells Toxicology letters 116, Merot, F., Seniuta, R., Benita, G. and Masson, Ph Method for quantifying cutaneous pigmentation in animals and preliminary study in humans. International Journal of Cosmetic Science. 14, Motoyoshi, K., Ota, Y., Takuma, Y. and Takenouchi, M Wrinkles from UVA exposure. 113, Phillips, B. J Development of cell culture techniques for assessment of the toxicity of plant products. Toxicology in vitro 10, Schallreuter, K. U. et al Regulation of melanin biosynthesis in the human epidermis by tetrahydrobiopterin. Science Shin, N. H. et al Oxyresveratrol as the potent inhibitor on dopa oxidase activity of mushroom tyrosinase. Biochememical And Biophysical Research Communications. 243, Shirota, S. et al Tyrosinase inhibitors from crude drugs. Biol. Pharm. Bull. 17,

9 20. Smith, J State of the industry: the Asia-Pacific cosmetics and toiletries sector, DCI Stern, M. Klausner, M., Alvarado, R., Renskers, K., and Dickens, M Evaluation of the EpiOcular tissue model as an alternative to the Draize eye irritation test. Toxicology in vitro 12,