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3 2017 한국육종학회 - 차세대 BG21 사업단 - GSP 사업단공동심포지엄일정 2017 년 7 월 5 일 ( 수 ) 2017 년 7 월 6 일 ( 목 ) 개회식 << 1 부 Plenary Session >> 중식 iii

4 << 2 부한국육종학회분과발표 >> 분과발표 -1: Genes and Molecular Breeding 분과발표 -2: Phenotypes and Agronomic Traits iv

5 << 2 부한국육종학회분과발표 >> 분과발표 -3: New Breeding Technology & Related Industry 분과발표 -4: Commercial Breeding and Seed Industry v

6 구두발표 -1: Oral Presentation of Young Breeders 포스터발표 vi

7 2017 년 7 월 7 일 ( 금 ) << 3 부 Concurrent session >> Concurrent session-1: 식물분자육종사업단 & GM 작물개발사업단 Concurrent session-2: 농생물게놈활용연구사업단 Concurrent session-3: Golden Seed Project Coffee Break vii

8 << 3 부 Concurrent session >> 구두발표 -2: Oral Presentation of Young Breeders 구두발표 -3: Oral Presentation of Young Breeders Coffee Break viii

9 << 4 부 Plenary Session >> ix

10 2017 Annual Symposium of Korean Society of Breeding Science 1 st day [July 5, 2017] 2 nd day [July 6, 2017] << Plenary Session >> x

11 2017 Annual Symposium of Korean Society of Breeding Science << Oral Presentation >> 3 rd day [July 7, 2017] << Concurrent Session >> << Plenary Session >> xi

12 Contents Plenary Session SYMP-01 Genome editing in rice and maize 4 Bing Yang SYMP-02 A genomic roadmap for breeding of tastier vegetables 6 Sanwen Huang SYMP-03 Targeted soybean breeding for maximum yield potential in different latitudes 8 Kristin Bilyeu SYMP-04 Functions and applications of innate immune receptors in plants 10 Kee Hoon Sohn SYMP-05 Food allergies, celiac disease and wheat sensitivities: biotechnology strategies to reduce the immunogenic potential of wheat flour 12 Susan B. Altenbach SYMP-06 Seeds market and biotechnology development in China 14 David Yeh SYMP-07 Crop breeding by genome-editing 16 Ju-Kon Kim xii

13 한국육종학회분과발표 OA-01 OA-02 Improvement of indica rice varieties using molecular breeding at the International Rice Research Institute (IRRI), Philippines 21 Sung-Ryul Kim, Joie Ramos, Sherry Lou Hechanova, G.D. Prahalada, Jeom-Sig Lee, Kshirod K. Jena The OsRFS encoding a CHD3/Mi-2 chromatin remodeling factor is involved in multiple developmental processes and ROS scavenging in rice 21 Kiyoon Kang, Sung-Hwan Cho, Nam-Chon Paek OA-03 Controlling flowering time by genetic and environmental factors in rice 22 Jinhuan Wei, Heebak Choi, Ping Jin, Yunfei Wu, Jinmi Yoon, Yang-Seok Lee, Taiyong Quan, Gynheung An OA-04 OA-05 An integrated multi-omics approach to investigate the ethylene and abscisic acid signaling in Glycine max leaves 22 Ravi Gupta, Cheol Woo Min, Ramesha H. Jayaramaiah, Sun Tae Kim Plastidic phosphoglucomutase and ADP-glucose pyrophosphorylase isoforms are essential for successful fertilization in rice 23 Sang-Kyu Lee, June-Seob Eom, Seok-Hyun Choi, Dae-Woo Lee, Jong-Seong Jeon OA-06 Development of the phenome and genome database for breeding 23 Keunpyo Lee, Junkyoung Choe, Younghoon Park, Jin-Hee Lim, Byung-Chun In, Sung-Chur Sim OB-01 Genome editing mediated trait development to improve the efficiency of bio-refinery from lignocellulosic biomass 24 Je Hyeong Jung, Ja Kyong Ko, Sun-Mi Lee OB-02 Rice RING E3 ligases : their roles in abiotic stresses 24 Cheol seong Jang OB-03 High-throughput phenotyping of plant using image analysis 25 Inchan Choi, Hyeonso Ji, JeongHo Baek, Song Lim Kim, Taek-Ryoun Kwon, Kyung-Hwan Kim OB-04 Isolation and identification of a novel Sg-9 gene responsible for DDMP saponin biosynthesis in soybean 25 Jong Tae Song xiii

14 OB-05 Genetic fine mapping and characterization of the sy-2 locus, responsible for low temperature sensitivity in pepper 26 Jelli Venkatesh, Li Liu, Min-Young Kang, Jin-Kyung Kwon, Yeong Deuk Jo, Jin-Ho Kang, Sota Koeda, Munetaka Hosokawa, Sandra Goritschnig, Byoung-Cheorl Kang OC-01 DIY CRISPR for plant genome editing 26 Sang-Tae Kim, Hyeran Kim, Je Wook Woo, Hee-Jung Sim, Jae-Young Yun, Hyo-min Ahn, Jahee Ryu, Min Kyung Choi, youjin Shin, Beum-Chang Kang, Suji Bae, Sunyoung Moon, Jin-Soo Kim, Sang-Gyu Kim OC-02 CRISPR system-mediated soybean genome editing 27 Hyeran Kim OC-03 TGsol: Open Innovation Platform 27 조성환, 이봉우, 김지은, 최준경, 김웅범, 오재은, 이정희 OC 유의수, 최범순, 김남훈, 이현오, 박미소, 이승욱 OC-05 Workflow solutions for current challenges in agricultural biotechnology 28 Lincoln Ong OC-06 Microfluidic genotyping system 29 정윤재, MS.Eng., MBA OD 최근진, 오동진, 양미희, 김태영, 김진욱 OD 강항구 OD 박형준 OD 유재흥, 이종훈, 안경구 xiv

15 OD 김명권 OD 조영일 한국육종학회구두발표 OE-1 Efficient FISH analysis method using synthetic oligomers for conserved high copy repeat blocks 35 Nomar Espinosa Waminal, Remnyl Joyce Pellerin, Sung-Min Youn, Tae-Jin Yang, Hyun Hee Kim OE-2 ms 1 35 정규미, 금보라, 이준대 OE-3 OE-4 Identification of SRK-homologous gene family and its use in development of a reliable haplotyping system for the S locus determining self-incompatibility in radish (Raphanus sativus L.) 36 Dong-Seon Kim, Juyeon Jung, Yeon-Ok Choi, Sunggil Kim Characterization of complete chloroplast genome of Peucedanum japonicum and its application for genetic diversity analysis. 36 Ho Jun Joh, Junki Lee, Sae Hyun Lee, Hyun-Oh Lee, Sang-Choon Lee, Tae-Jin Yang OE-5 Overexpression of YUCCA6 gene enhanced drought tolerance in transgenic soybean 37 Jin Sol Park, Hye Jeong Kim, Hyun Suk Cho, Jae Seong Kim, Wan Woo Yeom, Jae Yong Han, Dae-Jin Yun, Young Soo Chung OE-6 Transcriptome profiling of differentially expressed genes associated with secondary growth in radish (Raphanus sativus L.) 37 Goh Choe, Yogesh Gupta, Ana Cecilia Aliaga Fandino, Ji-Young Lee OE-7 Engineering rice with lower grain arsenic 38 Fenglin Deng, Naoki Yamaji, Jian Feng Ma, Enrico Martinoia, Youngsook Lee, Won-Yong Song xv

16 OE-8 OE-9 Enhanced thermo-tolerance in Arabidopsis thaliana through over-expression of CBF1 gene 38 Rupesh Tayade, Tien Dung Nguyen, Lee Dasom, Sung Aeong Oh, Soon Ki Park Complex genome structure of Panax ginseng revealed by ten BAC clone sequences obtained by 3rd generation SMRT sequencing platform using pooled DNA 39 Woojong Jang, Nam-Hoon Kim, Junki Lee, Nomar Espinosa Waminal, Sang-Choon Lee, Murukarthick Jayakodi, Hong-il Choi, Jee Young Park, Yeeun Jang, Tae-Jin Yang OE 이현숙, 전윤아, 강주원, 안상낙 OE-11 Soybean transgenic plants with AtKDA1 gene showed morphological change in plant height 40 Hye Jeong Kim, Hyun Suk Cho, Jae Seong Kim, Jin Sol Park, Wan Woo Yeom, Jae Yong Han, Jeong-Il Kim, Young Soo Chung OE 박현수, 백만기, 남정권, 신운철, 김춘송, 이건미, 박슬기, 조영찬, 김보경 OE-13 OE-14 Mutator based transposon display: Genetic Tool for Evolutionary and crop improvement studies in maize 41 Rahul Vasudeo Ramekar, Neha Samir Roy, Youn Sung Kim, Kyong Cheul Park, Ju Kyong Lee Chloroplast genome, nuclear ribosomal genes and polymorphic SSR markers derived from two whole genome sequence of Euonymus hamiltonianus individuals 41 Junki Lee, Shin-Jae Kang, Ho Jun Joh, Nam-Hoon Kim, Sang-Choon Lee, Woojong Jang, Hyun-Seung Park, Jee Young Park, Tae-Jin Yang OE-15 Development of high tryptophan japonica rice with good eating and grain quality 42 Franz Marielle Nogoy, Hye-Jung Lee, Marjohn Nino, Dal-A Yu, Me-Sun Kim, Sothea Ouk, Yu-Jin Jung, Kwon-Kyoo Kang, Yong-Gu Cho OE-16 Candidate gene analysis of plant height and antioxidant using NILs 42 Yun-A Jeon, Dong-Min Kim, Hyun-Sook Lee, Sun Ha Kim, Sang-Nag Ahn OE-17 Mapping by exome sequencing in wheat: a tall mutant case study 43 Youngjun Mo, Hans Vasquez-Gross, Luis Alejandro de Haro, Tyson Howell, Stephen Pearce, Jorge Dubcovsky xvi

17 한국육종학회포스터발표 PA-01 Molecular cloning, expression and characterization of a squalene synthase gene from grain amaranth (Amaranthus cruentus L.) 47 Young-Jun Park, Kazuhiro Nemoto, Kenichi Matsushima, Han-Yong Um, Jung-Hoon Choi, Chan-sung Oh, Tomotaro Nishikawa PA-02 Gene pyramiding of Sub1A and Pup1 in rice 47 Na-Hyun Shin, Joong Hyoun Chin, Soo-Cheul Yoo PA 지희정, 황태영, 이기원 PA-04 PCR 48 진성범, 박재호, 박석만, 김민주, 김호방, 윤수현 PA-05 Bx7 49 조성우, 김재덕, 강택규, 백연경, 박철수 PA-06 Glu-D1 y- 49 조성우, 김재덕, 강택규, 백연경, 박철수 PA-07 Identification of IDD7 and IDD14 mutants in Arabidopsis thaliana 50 Le Thi Dung, Hyun Uk Kim PA-08 LegExpress: A bioinformatic platform for transcriptome analysis in legume genomes 50 Joo-Seok Park, Chaeyoung Lee, Jin-Hyun Kim, Yoram Choi, Min-Gyun Jeong, Hong-Kyu Choi PA-09 LegCompara: A bioinformatic platform for interactive comparative genome analysis in legumes 51 Jin-Hyun Kim, Chaeyoung Lee, Joo-Seok Park, Yoram Choi, Min-Gyun Jeong, Hong-Kyu Choi PA-10 Alternative splicing events of drought-related genes under drought stress in maize 51 Kitae Song, Hyochul Kim, Kyung-Hee Kim, Jun-Cheol Moon, Jae Yoon Kim, Byung-Moo Lee PA-11 Development of Japonica advanced backcross lines having bacterial blight resistant genes from a cross between japonica and indica 52 Woo-Jae Kim, Jae-Beom Chun, Hyun-Su Park, Hyun-Soon Kim, Kyung-Ho Kang, Ji-Ung Jeong, Jae-Kwon Ko xvii

18 PA-12 Study in search for the heritable force driving phenotypic variation in soybean seed size 52 Sampathkumar Palaniswamy, Jagadeesh Sundaramoorthy, Gyu Tae Park, Jeong-Dong Lee, Hak Soo Seo, Jong Tae Song PA-13 Identification of the gene responsible for high and low inorganic phosphorus in soybean 53 Thi Hai Yen Nong, Jagadeesh Sundaramoorthy, Gyu Tae Park, Jeong-Dong Lee, Hak Soo Seo, Jong Tae Song PA-14 Identification of SNP markers in boysenberry using genotyping-by-sequencing 53 Juhyun Im, Woon Ji Kim, Sang Hun Kim, Seung Cheol Oh, Lan Cho, Si-Yong Kang, Sung-Hwan Jo, Bo-Keun Ha PA-15 Identification and characterization of new high stearic acid soybean mutant induced by gamma-ray irradiation 54 Sang Hun Kim, Juhyun Im, Woon Ji Kim, Ryuli Gang, Si-Yong Kang, Bo-Keun Ha PA-16 Human Bone Morphogenetic Protein-2 (hbmp-2) 54 이주영, 김용호 PA-17 PA-18 Molecular studies of mutations induced by proton beam irradiation in soybean using genotyping-by-sequencing (GBS) 55 Woon Ji Kim, Juhyun Im, Sanghun Kim, Bo-Keun Ha A genome-wide association study of amino acids in core collection of wild soybean (Glycine soja) 55 Woon Ji Kim, Juhyun Im, Sanghun Kim, Ju Seok Lee, Sungtaeg Kang, Soon-Chun Jeong, Jung-Kyung Moon, Bo-Keun Ha PA 서정현, 강범규, 김현태, 신상욱, 김홍식, 백인열, 한원영, 윤홍태, 이병원, 고종민, 곽도연 PA-20 PA-21 PA-22 PA-23 Genetic diversity analysis in Perilla crop from northern areas of china based on SSR molecular markers 56 Shi Jun Ma, Kyu Jin Sa, Tak-Ki Hong, Ju Kyong Lee Bulk segregant analysis (BSA) for drought stress in maize inbred lines using SSR markers 57 Muhammad Qudrat Ullah Farooqi, Shi Jun Ma, Kyu Jin Sa, Ju Kyong Lee Construction of a Linkage map and mapping of QTLs related to agronomic traits in maize with help of Mutator based transposon markers and simple sequence repeat markers 57 Rahul Vasudeo Ramekar, Kyong-Cheul Park, Kyu Jin Sa, Ju Kyong Lee Understanding genetic differentiation and association mapping of agronomic traits in waxy and common maize based on mutator transposon display 58 Rahul Vasudeo Ramekar, Kyong-Cheul Park, Kyu Jin Sa, Ju Kyong Lee xviii

19 PA 박노봉, 남민희, 김상열, 여운상, 이지윤, 권오덕, 박동수, 이종희, 조준현, 송유천, 오성환 PA-25 PA-26 PA-27 PA-28 Effect of sound wave on the expression of ethylene biosynthesis-related genes through regulation of transcription factors RIN and HB-1 59 Mi Jeong Jeong, Joo Yeol Kim, Soo In Lee, Jin A Kim Phylogenic analysis of 246 Korean rice varieties using core sets of microsatellite markers 59 Yong-Sam Kwon, Norvie L. Manigbas, Do-Hon Kim, Gihwan Yi Selection of highly informative SNP markers for Sesame (Sesamum indicum) cultivar identification 60 Jin-Kee Jung, Eun-Jo Sim, Seul-Ki Lim, Dong Min Kim, Seung-In Yi Genome-wide identification and characterization of cold stress tolerance and early flowering in rice using next generation sequencing technology 60 Songhwa Chae, Joung Sug Kim, Kyong Mi Jun, Sang-Bok Lee, Yeon-Ki Kim PA-29 Transcriptomic profiling of pear plant defense responses against Venturia nashicola infection 61 Il-Sheob Shin, Jaean Chun, Yoon-Kyeong Kim PA-30 QTL Detection for Durable Resistance to Blast in a Japonica Variety, Palgong 61 Man-Kee Baek, Jong-Min Jeong, Hyun-Su Park, Soon-Wook Kwon, Woo-Jae Kim, Jeong-Kwon Nam, Seul-Gi Park, Choon-Song Kim, You-Chun Song, Bo-Kyeong Kim, Young-Chan Cho PA 김정주, 신운철, 김우재, 박현수, 강위금, 남정권, 백만기, 조영찬, 김보경, 김대식 PA-32 Characterization of breeding materials with diverse grain size and shape in japonica rice 62 Hyun-Su Park, Man-Kee Baek, Jeong-Kwon Nam, Woon-Chul Shin, Choon-Song Kim, Gun-Mi Lee, Seul-Gi Park, Young-Chan Cho, Bo-Kyeong Kim PA 강범규, 김현태, 서정현, 신상욱, 김홍식, 윤홍태, 이영훈, 오은영, 정찬식, 오인석, 곽도연 PA 강범규, 김현태, 서정현, 신상욱, 김홍식, 최만수, 윤홍태, 고종민, 백인열, 한원영, 이영훈, 이병원, 오기원, 김현영, 곽도연 PA 최유현, 김형태, 임기병 PA-36 Soybean transgenic plants with AtKDA1 gene showed morphological change in plant height 64 Hye Jeong Kim, Hyun Suk Cho, Jae Seong Kim, Jin Sol Park, Wan Woo Yeom, Jae Yong Han, Jeong-Il Kim, Young Soo Chung xix

20 PA-37 Overexpression of YUCCA6 gene enhanced drought tolerance in transgenic soybean 65 Jin Sol Park, Hye Jeong Kim, Hyun Suk Cho, Jae Seong Kim, Wan Woo Yeom, Jae Yong Han, Dae-Jin Yun, Young Soo Chung PA-38 QTL identification for seed storability in rice 65 Dong-Min Kim, Eun-Jo Shim, Seung-Eun Park, Shin-Sook Park, Gyu-Chan Shim, Seung-In Yi PA-39 Human drug niclosamide can be used to inhibits Xanthomonas oryzae-induced rice leaf blight 66 Jun Soo Kwak, Won Joo Shin, Beom Seok Choi, Hak Soo Seo PA-40 Seed germination is controlled by sumoylation in Arabidopsis 66 Sang Woo Park, Woo Sub Kim, Hak Soo Seo PA-41 AtSIZ1-mediated Abiotic stresses are regulated by E3 ubiquitin ligase activity of COP1. 67 Joo Yong Kim, Kyu Ho Lee, Hak Soo Seo PA-42 Isolation and characterization of putative F-box proteins in wheat 67 Min Jeong Hong, Dae Yeon Kim, Joon-Woo Ahn, Sang Hoon Kim, Hong-Il Choi, Si-Yong Kang, Yong Weon Seo, Jin-Beak Kim PA-43 Pinkie Girl 68 박종택, 정재아, 박상근, 김미선 PA-44 Identification of QTLs for tolerance to hypoxia during germination in rice 68 Suk-Man Kim, Kyeong-Ho Kang, Jong-Min Jeong, Russell Reinke PA-45 High temperatures induce diploid pollen and male sterility in Arabidopsis 69 Tien Dung Nguyen, Sung Aeong Oh, Soon Ki Park PA-46 PA-47 PA-48 Identification and molecular characterization of DDMP saponin biosynthesis gene in soybean 69 Jagadeesh Sundaramoorthy, Gyu Tae Park, Seokhyung Lee, Jeong-Dong Lee, Hak Soo Seo, Jong Tae Song Identification and evaluation of abiotic stress tolerant genes in Arabidopsis thaliana and develop transgenic rice for heat stress tolerance 70 Rupesh Tayade, Tien Dung Nguyen, Lee Dasom, Sung Aeong Oh, Ki Hong Jung, Soon Ki Park Identification of genetic basis causing a cytokinesis defect in Arabidopsis mutant phenotype by map-based cloning 70 Saima Akhter, Ha Thi Lieu, Rupesh Tayade, Sung Aeong Oh, Soon Ki Park PA-49 background selection SNP 71 김진희, 이은수, 안율균, 김도선, 홍종필, Abinaya Manivannan, 최승국 xx

21 PA-50 PA-51 Molecular genetic mapping of Chili veinal mottle virus (ChiVMV) resistance genes in pepper 71 Joung-Ho Lee, Jeong-Tak An, Koeun Han, Seula Choi, Muhammad Irfan Siddique, Byoung-Cheorl Kang Evaluation of phenotypic variants and identification of non-pungent mutants among EMS-treated M 2 populations 72 Muhammad Irfan Siddique, Koeun Han, Byoung-Cheorl Kang PA-52 QTL mapping for inward rolling of leaf blade in rice using RIL population 72 Su Jang, Hyekyung Son, Taejun Kim, Jinwoo Lee, Hee-Jong Koh PA-53 Fine-mapping and characterization of a new light dependent incomplement dominant lesion mimic mutant in rice (Oryza sativa L.) 73 Zhuo Jin, Jin seok Moon, Somyung Lee, Hee-Jong Koh PA-54 The evolution of tomato mitochondrial genomes and intracellular gene transfer in tomato 73 Hyoung Tae Kim, Je Min Lee PA 최재근, 박종열, 장은하, 류시환, 서영호, 박기진, 남궁민, 윤석원, 용우식, 최준근 PA-56 RNA sequencing and gene network analysis in rice irradiated with gamma-rays and ion-beams 74 Hong-Il Choi, Sung Min Han, Sun-Goo Hwang, Cheol Seong Jang, Soon-Jae Kwon, Joon-Woo Ahn, Yeong Deuk Jo, Sang Hoon Kim, Si-Yong Kang Jin-Baek Kim PA 안억근, 이상복, 이점호, 장재기, 원용재, 이정희, 정국현, 하운구, 정응기, 김명기, 정종민, 서정필, 양창인 PA-58 MutMap 75 안억근, 홍하철, 정국현, 원용재, 현웅조 PA-59 Construction and application of functional gene modules to regulatory pathways in rice 76 Woo-Jong Hong$, Anil Kumar Nalini Chandran$, Jong-Seong Jeon, Ki-Hong Jung PA-60 PA-61 Quadratic code system for genetic identification of rice cultivars using InDel markers specific to dense variation blocks 76 Hyangmi Park, Yongjae Won, Kukhyun Jung, Hyun Ungjo, Hacheol Hong, Yulho Kim Quantitative trait loci(qtls) for gummy stem blight resistance using Bi-parental populations in watermelon 77 Eun Su Lee, JinHee Kim, Yul-Kyun Ahn, Jong Pil Hong, Abinaya Manivannan, Tae Sung Park, Minkyoung Kim, Do-Sun Kim PA-62 Genome-wide identification of the dehydrin genes in the Cucurbitaceae species 77 Sang-Choon Lee, Won-Kyung Lee, Ho Jun Joh, Inseo Kim, Jaehyeon Jeon, Sae Hyun Lee, Tae-Jin Yang, Kihwan Song xxi

22 PA-63 SSR 4 78 김민주, 윤수현, 박재호, 박석만, 진성범 PA 정재아, 신학기, 김미선, 박종택 PA 정재아, 이은혜, 신학기, 김미선, 박종택 PA-66 Genotyping and bioinformatics analysis of single nucleotide polymorphism related to powdery mildew resistance in Capsicum baccatum and Capsicum annuum 79 Abinaya Manivannan, Yul-Kyun Ahn, Tae-Hwan Jun, Eun-Young Yang, Sena Choi, Jinhee Kim, Eun-Su Lee, Do-Sun Kim PA-67 Sequencing and de novo assembly of diploid perilla genome 80 Seon-Hwa Bae, Kyeong-Seong Cheon, Myoung-Hee Lee, Ki-Won Oh, Jeong-Hee Lee, Ung-Han Yoon, Jundae Lee, Tae-Ho Kim PA-68 PA-69 PA-70 Genome-wide identification of the SOD, APX, and CAT gene families in the Cucurbitaceae species 80 Won-Kyung Lee, Sang-Choon Lee, Ho Jun Joh, Inseo Kim, Jaehyeon Jeon, Sae Hyun Lee, Tae-Jin Yang, Kihwan Song Molecular identification of Allium ochotense and A. microdictyon using multiplex-pcr based on single nucleotide polymorphisms 81 Yong Bog Kim, Rahul Vasudeo Ramekar, Byong Gon Choi, Ju Kyong Lee, Ik Young Choi, Sun Gang Choi, Kyong Cheul Park Construction of high-resolution map and analysis of stem related to QTL using Milyang23/Gihobyeo recombinant inbred lines 81 Ye-Ji Lee, Kyeong-Seong Cheon, Eun-Ju Byeon, Hyeonso Ji, Gang-Seob Lee, Ung-Han Yoon, Nam-In Hyung, Tae-Ho Kim PA-71 qvdt11, a major QTL related to drought tolerance as stable tiller formation in rice 82 Su-Min Jo, Tae-Heon Kim, Dongjin Shin, Saes-Beul Lee, Ji-Yoon Lee, Sang-Ik Han, Jun-Hyeon Cho, Kyung-Min Kim, Jong-Hee Lee, You-Chun Song, Dong-Soo Park, Myung-Kyu Oh PA 김현태, 강범규, 한원영, 이병원, 이영훈, 최만수, 서정현, 고종민, 윤홍태, 오기원, 하태정, 곽도연 PA-73 Atomato flowering gene SELF PRUNING 5G promotes day-neutrality and early yield 83 Soon Ju Park, Sebastian Soyk, Jong Hyang Bae, José M Jiménez-Gómez, Zachary B Lippman PA-74 JMJ30-mediated H3K9me3 demethylation drives tissue identity changes to promote cellular reprogramming in Arabidopsis 83 Kyounghee Lee, Ok-Sun Park & Pil Joon Seo xxii

23 PA-75 The MIEL1 E3 ubiquitin ligase negatively regulates cuticular wax biosynthesis in Arabidopsis stems 84 Hong Gil Lee, Juyoung Kim, Mi Chung Suh, Pil Joon Seo PA 김경민, 신동진, 김경훈, 박효진, 권유리, 현종내, 오명규 PA-77 PA-78 Resequencing of Gayabyeo, a Tongil-type brown planthopper (BPH) resistant rice variety, and development of single nucleotide polymorphism (SNP) markers 85 Hyun-Ju Kang, Eokkeun Ahn, Song Lim Kim, Inchan Choi, Jeongho Baek, Kyung-Hwan Kim, Hyeonso Ji Genetic analysis of four dense green leaf mutant lines of rice and their genome resequencing 85 Jun Oh, Hyun-Ju Kang, Song Lim Kim, Jeongho Baek, Inchan Choi, Kyung-Hwan Kim, Hyeonso Ji PA 배민지, 진영돈, 안동춘, 정용모, 최시림, 홍광표, 황주천 PA-80 Detection of QTLs in interspecific Perilla citriodora x P. hirtella mapping population 86 Myoung Hee Lee, Ki Won Oh, Myung Sik Kim, Jung In Kim, Eun Young Oh, Suk Bok Pae, Un Sang Yeo, Tae-Ho Kim, Jeong Hee Lee, Do Yeon Kwak, Yong Chul Kim PA-81 High oleic acid PE451, PE2742, PE 박은영, 이정동 PA-82 AR 87 도재왕, 윤재복, 박석진 PA-83 Mutation of SPOTTED LEAF4 (SPL4) encoding a microtubule severing protein produces reactive oxygen species (ROS) and delays leaf senescence in rice 88 Giha Song, Da-Yea Park, Nam-Chon Paek PA-84 Mastering of terpenoid metabolic pathway via rice MYB transcription factor, OsMYBR22 88 Hyung-Keun Ku, Ye Sol Jeong, Min-Kyoung You, Dongho Lee, Sun-Hyung Lim, Sun-Hwa Ha PA-85 PA-86 Genome-wide DNA polymorphisms analysis of rice mutants by gamma-ray irradiation based on Whole genome re-sequencing 89 Lee Jae Woo, Cheol Seong Jang The expression of putative genes related to plant growth in dwarf soybean derived from crossing of G. max and G. soja. 89 Yong-Wook Ban, Taewook Kim, Doori Park, Chanseok Shin, Ik-young Choi PA-87 (Ligularia fischeri) microsatellite 90 안혜림, 김영미, 조아르나, 권용락, 서용원, 정지희 xxiii

24 PA-88 PA-89 PA-90 PA-91 Assessment of parental genome in Asiatic x L. longiflorum Hybrids through genomic in situ hybridization analysis 90 Fahad Ramzan, Hyoung Tae Kim, Adnan Younis, Young Kwan Jo, Choi Yu Hyeon, Ki-Byung Lim OsPhyB-mediating novel regulatory pathway for drought tolerance in rice root identified by a global RNA-Seq transcriptome analysis of rice genes in response to water deficiencies 91 Yo-Han Yoo, Anil Kumar N.C, Jong-Chan Park, Yun-Shil Gho, Sang-Won Lee, Gynheung An, Ki-Hong Jung Callus induction and plant regeneration from immature zygotic embryos of various maize genotypes (Zea mays L.) 91 Joon Ki Hong, Ki Jin Park, Gang-Seob Lee, Dool Yi Kim, Ju-Kon Kim, Seung Bum Lee, Eun Jung Suh, Kyung-Hwan Kim, Yeon-Hee Lee Identifying cold stress related genes of rice (Oryza sativa L.) at early young microspore stage using Genome-wide association study 92 Mijeong Kim, Taegyu Kim, Yoonjung Lee, Jisu Choi, Giwon Cho, Joohyun Lee PA-92 Transcriptome profiling of fruit maturity genes in sweet persimmon (Diospyros kaki Thunb.) 92 Eun-Gyeong Kim, Ji-Young Son, Yeo-Ok, Park, Young-Han Lee, Doo-Sang Park, Youngmi Choi, Joonyup Kim, Younghoon Park PA-93 Proteome analysis of leaves and roots of sorghum under copper stress 93 Swapan Kumar Roy, Seong-Woo Cho, Soo Jeong Kwon, Kabita Sarker, Zhanguo Xin, Sun-Hee Woo PA-94 Overexpression of ICE1, a regulator of cold acclimation, enhances cold tolerance in transgenic Arabidopsis thaliana 93 Zhi-Fang Zuo, Hana Jeong, Hyeon-Jin Sun, Mi-Young Park, Dae-Hwa Yang, Chang-Ho Eun, Hong-Gyu Kang, Hyo-Yeon Lee PA-95 Differential proteomic analysis of cadmium-responsive proteins in leaves and roots 94 Swapan Kumar Roy, Seong-Woo Cho, Soo Jeong Kwon, Kabita Sarker, Zhanguo Xin, Sun-Hee Woo PA-96 (JG21-MJ) 94 정하나, 좌지방, 선현진, 양대화, 강홍규, 이효연 PA 하건수, 송윤호, 조윤상, 이지우 PA-98 Comparison of transcriptomic changes in Miscanthus sinensis under salt stress 95 Minjung Yook, Soo-Hyun Lim, Hyejin Yu, Do-Soon Kim PA-99 Genetic diversity and relationship analysis of Angelica gigas using genetic SSR markers 96 Yurry Um, Dae Hui Chung, Gwang Hun Park, Jinsu Gil, Yi Lee, Chungryul Jung, Kwon Seok Jeon, ManJo Kim, Yunmi Park xxiv

25 PA-100 The bicistronic expression of two non-mammalian Viral 2A sequences in plants using golden rice phenotype 96 Ye Sol Jeong, Young Joo Jung, Hyung-Keun Ku, Jae Kwang Kim, Kyoung Bok Lee, Mi Ran Ko, Min Kyoung You, Sun-Hyung Lim, Ju-Kon Kim, Dong ho Lee, Sun-Hwa Ha PA-101 Growth characteristics and amino acids composition of quality protein maize lines 97 Hwan-Hee Bae, Beom-Young Son, Jung-Tae Kim, Jin-Seok Lee, Sang Gon Kim, Seong-Bum Baek PA-102 GASA 97 최현모, 배은경, 이효신, 최영임 PA-103 A simple, rapid and systematic method for the developed GM rice analysis 98 So-Young Kim, Min Kang, Hye-min Lim, Jung-Il Cho, Hyeon-so Ji, Kang-Hyun Lee, Soo-Chul Park, Gang-Seob Lee PA-104 PA-105 Establishment of hairy root cultures by Agrobacterium rhizogenes-mediated transformation of ginseng (Panax ginseng) for ginsenoside production 98 Min Jung Kim, Van Binh Nguyen, Vo Ngoc Linh Giang, Yun Sun Lee, Tae-Jin Yang A putatively stress-related gene BrTSR53 isolated from Chinese cabbage (Brassica rapa) confers salt stress tolerance in Yeast 99 Min Kang, So-Young Kim, Hye-min Lim, Jung-Il Cho, Hyeon-so Ji, Kang-Hyun Lee, Soo-Chul Park, Gang-Seob Lee PA-106, 99 김동용, 박소연, 왕헝, 권순욱 PA-107 Development of chloroplast DNA markers for classification of the collected species and for ecological restoration in Rhus chinensis (Anacardiaceae) 100 Inseo Kim, Yun Sun Lee, Jee Young Park, Ho Jun Joh, Hyun Oh Lee, Sang-Choon Lee, Tae-Jin Yang PA-108 A nonsense mutation of Capsanthin/capsorubin synthase controlling orange pepper fruit 100 Jeong Eun Kim, Hee Ju Yoo, Byung-Cheorl Kang, Je Min Lee PA-109 Inferring the genetic determinants of fruit colors in tomato by carotenoid profiling 101 Hee Ju Yoo, Gyu-Myung Lee, Chang-Sik Oh, Inhwa Yeam, Je Min Lee PA-110 Integrative transcriptomic and functional analyses to unveil distinct genetic influences on fruit ripening between pepper and tomato 101 Gyu-Myung Lee, Hee Ju Yoo, InhwaYeam, DoilChoi, Je Min Lee PA 김재희, 박영기, 김세현 PA 박영기, 김재희, 김세현 xxv

26 PA-113 Characterization of low-temperature germinability QTL, qltg1 103 Sun Ha Kim, Kyu-Chan Shim, Hyun-Sook Lee, Anh Quynh Le, Sang-Nag Ahn PA-114 PA-115 Genetic diversity, population structure, and core collection of black soybean accessions with green cotyledon using 6K SNP marker 103 Ji Yun Lee, Hong Jib Choi, Chang Suk Huh, Jeong Suk Bae, Se Jong Kim, Jeong Dong Lee Development of high resolution genetic map for Onion (Allium cepa L.) using reference free Genotyping-by-sequencing (GBS) and SNP assays 104 Jinkwan Jo, Preethi M Purushotham, Koeun Han, Heung-ryul Lee, Gyoungju Nah, Byoung-Cheorl Kang PA-116 Functional analysis of rice OsXRI5 gene related to bacterial blight 104 Hye Seon Kim, Seok-Jun Moon, Kyong Sil Lee, Duk-Ju Hwang, Il-Pyung Ahn, Shin-Chul Bae, Myung Ok Byun, Sang Ryeol Park PA-117 PA-118 PA-119 PA-120 Diversity in composition and molecular weight distribution of endosperm proteins among Ethiopian tetraploid wheat genotypes 105 Daniel Hailegiorgis, Chong Ae Lee, Song Joong Yun Analysis of QTL associated with flag-leaf angle using a doubled haploid genetic map in rice (Oryza sativa L.) 105 Jeong-Gwan Ham, Sopheap Yun, Rahmatullah Jan, A-Ra Cho, Jun-Hoi Kim, Xiao-Xuan Du, Kyung-Min Kim Construction of genetic map using EST-SSR markers in sweetpotato (Ipomoea batatas (L.) Lam) 106 Jin-Hee Kim, Jun-Hoi Kim, Xiao-Xuan Du, Sopheap Yun, Kyung-Min Kim Genetic analysis of chlorophyll content and leaf senescence using progeny from an interspecific cross in rice (Oryza sativa L.) 106 Kyu-Chan Shim, Luong Ngoc Ha, Sun Ha Kim, Yun-A Jeon, Hyun-Sook Lee, Xin Lu, Sang-Nag Ahn PA-121 SNP 107 안영은,, 허온숙, 노나영, 고호철, 전영아, 성정숙, 이주희, 백형진, 이준대 PA-122 Genome-wide association study for detection of disease resistance genes in grape vine 107 Kyo-Sang Lee, Hyun A Jang, Sang-Keun Oh PA-123 Genetic variations on florigen related genes in tomato. 108 Min-Sung Kang, Jong Hyang Bae, Soon Ju Park PA-124 Development of freezing tolerance molecular markers from Brassica oleracea 108 Hayoung Song, Yoonkang Hur PA-125 Development of genetic markers in Korean cultivated peanut(arachis hypogaea L.) by resequencing 109 Daewoong Lee, Tae-Hwan Jun xxvi

27 PA-126 Identification of novel pungency regulatory genes in the fatty-acid biosynthesis pathway in Capsicum annuum 109 Juhun Lee, Min-Young Kang, Koeun Han, Doyeon Hwang, Byoung-Cheorl Kang PA-127 QTL 110 이현숙, 심규찬, 김선하, 송원용, 안상낙 PA-128 Development of genetic maps of Allium cepa using genotyping-by-sequencing 110 Daewoong Lee, YongSuk Chung, Changsoo Kim, Tae-Hwan Jun PA-129 Identification of candidate genes associated with bacterial wilt resistance in pepper (Capsicum annuum L.) 111 Daewoong Lee, YangJae Kang, Yul-Kyun Ahn, Taehwan Jun PA-130 GWAS for rice seed germinability under low temperature condition. 111 Ah-Rim Lee, Heng Wang, Seong-Gyu Jang, Da-Eun Im, Soon-Wook Kwon PA-131 PA-132 PA-133 Compartmentation of rice preyltransferases for terpene precursors (GPP, FPP, GGPP, and SPP) in greening leaf protoplasts 112 Min Kyoung You, Sun-Hwa Ha A new semi-dwarf and white grain wheat variety, Baekkang with good baking bread quality 112 Chon-Sik Kang, Kyeong-Hoon Kim, Young-Keun Cheong, Jae-Han Son, Jong-Chul Park, Yang-Kil Kim, Kyong-Ho Kim, Tae-Il Park, Young-Mi Yoon, Ouk-Kyu Han, Gi-Heung Hong, Jin-Kyeong Choi, Jae-Ki Song, Jeong-Suk Bae, Bo-Kyeong Kim, Chulsoo Park A new semi-dwarf and red grain wheat variety, Saekeumkang with resistance to pre-harvest sprout, fusarium head blight and good making noodles quality 113 Chon-Sik Kang, Kyeong-Hoon Kim, Young-Keun Cheong, Jae-Han Son, Jong-Chul Park, Yang-Kil Kim, Kyong-Ho Kim, Tae-Il Park, Young-Mi Yoon, Ouk-Kyu Han, Gi-Heung Hong, Jin-Kyeong Choi, Jae-Ki Song, Jeong-Suk Bae, Bo-Kyeong Kim, Chulsoo Park PA-134 Screening techniques for stress tolerance against drought and salinity in rice seedlings 113 Jae-Young Song, Seon-Kyeong Song, Dal-A Yu, Me-Sun Kim, Sothea Ouk, Kwon Kyoo Kang, Yong-Gu Cho PA-135 PA-136 A new wheat variety, Johan with winter hardiness resistance, early maturity, high yield and good noodle quality 114 Chon-Sik Kang, Kyeong-Hoon Kim, Young-Keun Cheong, Jae-Han Son, Jong-Chul Park, Yang-Kil Kim, Kyong-Ho Kim, Tae-Il Park, Young-Mi Yoon, Ouk-Kyu Han, Gi-Heung Hong, Jin-Kyeong Choi, Jae-Ki Song, Jeong-Suk Bae, Bo-Kyeong Kim, Chulsoo Park Functional properties of an alternative, tissue-specific promoter for rice NADPH-dependent dihydroflavonol reductase 114 Joonki Kim, Hye-Jung Lee, Wricha Tyagi, Michael Kovach, Megan Sweeney, Susan McCouch, Yong-Gu Cho xxvii

28 PA-137 PA-138 PA-139 A new long spike and numerous wheat variety, Taejoong with resistance to lodging and fusarium head blight and good noodle quality 115 Chon-Sik Kang, Kyeong-Hoon Kim, Young-Keun Cheong, Jae-Han Son, Jong-Chul Park, Yang-Kil Kim, Kyong-Ho Kim, Tae-Il Park, Young-Mi Yoon, Ouk-Kyu Han, Gi-Heung Hong, Jin-Kyeong Choi, Jae-Ki Song, Jeong-Suk Bae, Bo-Kyeong Kim, Chulsoo Park Comparative expression analysis of rice and Arabidopsis peroxiredoxin genes suggests conserved or diversified roles between the two species and leads to the identification of tandemly duplicated rice peroxiredoxin genes differentially expressed in seeds 115 Yun-Shil Gho, Sun-A Park, Sung-Ruyl Kim, Anil Kumar Nalini Chandran, Gynheung An, Ki-Hong Jung Transcriptome profiling of flowering genes in Chinese cabbage and comparison with the radish flowering gene expression 116 Won Yong Jung, Areum Lee, Youn-Sung Kim, Hye Sun Cho PA-140 Xanthomonas oryzae pv. oryzae triggers complex transcriptomic defense network in rice 116 Marjohn Nino, Franz M. Nogoy, Me-Sun Kim, Jae-Young Song, Kwon-Kyoo Kang, Yong-Gu Cho PA-141 PA-142 PA-143 PA-144 PA-145 PA-146 Genetic diversity analysis of pear (Pyrus spp.) germplasm based on genotyping-by- sequencing 117 Keumsun Kim, Sewon Oh, Hyeondae Han, Kidong Hwang, Min-Gi Lee, Suji Ahn, Jiyeon Kim, Yoon-Kyeong Kim, Daeil Kim Comparative transcriptional profiles of compatible and incompatible interactions between Xanthomonas oryzae pv. oryzae and rice 117 Marjohn Nino, Joon ki Kim, Franz M. Nogoy, Dal-A Yu, Sothea Ouk, Jae-Young Song, Kwon-Kyoo Kang, Yong-Gu Cho Comparative analysis of phylogenetic trees assessed by SSR and SNP markers in Asian pears (Pyrus spp.) 118 Keumsun Kim, Kidong Hwang, Sewon Oh, Hyeondae Han, Min-Gi Lee, Suji Ahn, Jiyeon Kim, Yoon-Kyeong Kim, Daeil Kim Analysis of genetic diversity and population structure for Actinidia arguta using SNPs derived from genotyping-by-sequencing 118 Sewon Oh, Keumsun Kim, Hyeondae Han, Kidong Hwang, Min-Gi Lee, Suji Ahn, Jiyeon Kim, Mockhee Lee, Daeil Kim Identification of genetic structure for kiwifruits (Actinidia spp.) using genotyping-by- sequencing 119 Sewon Oh, Keumsun Kim, Hyeondae Han, Kidong Hwang, Min-Gi Lee, Suji Ahn, Jiyeon Kim, Mockhee Lee, Daeil Kim Molecular characterization of TaGAST1, one of wheat GAST family of small polypeptides during spike development 119 Jae Yoon Kim, Chang-Ho Kim, Yong Weon Seo xxviii

29 PA-147 PA-148 PA-149 Assessment of Genetic relationship using genome-wide single nucleotide polymorphisms developed by genotyping-by-sequencing in apples (Malus spp.) 120 Hyeondae Han, Keumsun Kim, Sewon Oh, Kidong Hwang, Min-Gi Lee, Suji Ahn, Jiyeon Kim, Sunheum Cho, Daeil Kim Use of SNP derived CAPS markers for identification of apple sports based on genotyping-bysequencing 120 Hyeondae Han, Keumsun Kim, Sewon Oh, Min-Gi Lee, Kidong Hwang, Jiyeon Kim, Suji Ahn, Sunheum Cho, Daeil Kim Tissue specific roles of 4-hydroxy-3-methylbut-2-enyl diphosphate reductases on rice terpenoids metabolism 121 Yeo Jin Lee, Min Kyoung You, Sun-Hwa Ha PA-150 BA NAA, 121 양환래, 박선경, 이상희, 김종보 PA-151 PA-152 ERF group VII genes predicted as candidates of terpene synthesis regulator under blue light 122 Heebak Choi, Sun-Hwa Ha Genetic variation architecture of mitochondrial genome reveals the differentiation in Korean landrace and weedy rice 122 Wei Tong, Qiang He, Yong-Jin Park PA-153 Lectures of statistical models and population genetics for crop breeding 123 Kyu-Won Kim, Soon-Chun Jeong, Ji-Hye Hwang, Hye-Won Chang, Hee-Jong Koh, Jung-Kyung Moon, Yong-Jin Park PA-154 PA-155 Identification of candidate defense genes for rice blast supported by orthology identification, eqtl mapping and GWAS 123 Kyu-Won Kim, Won-Hee La, Il-Pyung Ahn, Yong-Jin Park The development of algorithms, to enable comparative genomic analysis from many-to-many orthologs. 124 Kyu-Won Kim, Jung-Rye Nam, Yong-Jin Park PA-156 Construction of rice haplotype/indel information for rice functional study 124 Kyu-Won Kim, Jung-Rye Nam, Yong-Jin Park PA-157 PA-158 A comparison of pipelines for quantifying transcript expression from RNA-Seq data of Korean rice core collection 125 Jung-Rye Nam, Kyu-Won Kim, Yong-Jin Park Tolerance of anaerobic conditions caused by flooding during direct seeding and germinated seedling 125 Win Htet Oo, Yong-Jin Park xxix

30 PA-159 Transcriptome wide association studies reveal complex regulation network of rice eating and cooking quality 126 Qiang He, Kyu-Won Kim, Weiguo Zhao, Yong-Jin Park PA 최홍집, 오태영, 허창석, 이지윤, 배정숙, 김세종 PA-161 CRISPR/Cas9 127 김미진, 문기범, 사미나단수브라야, 장지영, 배상수, 이긍주, 김현순 PA-162 PA-163 Transcriptome analysis of putative MYB-bHLH-WD40 transcription factors in purple and yellow seeds of wheat (Triticum aestivum L.) during seed development 127 Paulina Calderón Flores, Dae Yeon Kim, Yong Weon Seo Differential gene expression of transcription factors related to the flavonoid pathway in the transcriptome of yellow and purple seeds of wheat (Triticum aestivum L.) during seed development 128 Paulina Calderón Flores, Dae Yeon Kim, Yong Weon Seo PA-164 Transcript analysis during wheat grain filling period under the heat stress 128 Chan Seop Ko, Meong Kyu Ou, Jong Nae Hyun, Kyung Hun Kim, Jin Baek Kim, Min Jeong Hong, Yong Weon Seo PA-165 Transcript and protein expression analysis of colored-grain wheat during grain development 129 Dae Yeon Kim, Min Jeoug Hong, Yong Weon Seo PA-166 Genetic analysis of dwarf wheat mutant generated by gamma ray irradiation 129 Eun Jeong Lee, Jin Seok Yoon, Yong Weon Seo PA-167 Development and flowering in wheat relative to vernalization period 130 Cheol Won Lee, Yong Weon Seo PA-168 RNA-seq analysis of wheat-rye translocations under cold stress 130 Woo Joo Jung, Yong Weon Seo PA-169 qcrs12, a major QTL related to cold tolerance at seedling stage in rice 131 Tae-Heon Kim, Su-Min Jo, Saes-Beul Lee, Ji-Yoon Lee, Sang-Ik Han, Jun-Hyeon Cho, Jong-Hee Lee, You-Chun Song, Dong-Soo Park, Myung-Kyu Oh, Dongjin Shin PA-170 Improved method for reliable HMW-GS identification by RP-HPLC and SDS-PAGE in common wheat cultivars 131 Jong-Yeol Lee, You-Ran Jang, Susan B Altenbach, Sun-Hyung Lim, Young-Mi Kim PA-171 Proteomic analysis of gliadin fractions in a common wheat 132 Jong-Yeol Lee, You-Ran Jang, Sun-Hyung Lim, Kyoungwon Cho xxx

31 PA-172 PA-173 PA-174 PA-175 PA-176 RNA interference-mediated simultaneous suppression of seed storage proteins in rice grains 132 Jong-Yeol Lee, You-Ran Jang, Sun-Hyung Lim, Kyoungwon Cho Enhancing of flower color with combinatorial expression of transcription factor genes, mpap1 and B-Peru 133 Da-Hye Kim, Jung Ae Park, Sung Ok Park, Sang-Kyu Park, Jong-Yeol Lee, Sun-Hyung Lim Chromosomal assignment of gliadin fractions in Chinese spring and its aneuploid lines using RP-HPLC, SDS-PAGE, A-PAGE and 2-DGE 133 You-Ran Jang, Sun-Hyung Lim, Jong-Yeol Lee Accurate and reliable analysis of LMW-GS alleles using Aroona NILs and standard cultivars 134 Su-Jin Cho, Jin Sun Kim, You-Ran Jang, Sun-Hyung Lim, Jong-Yeol Lee Cloning and functional characterization of floral scent full length genes in Cymbidium Cultivar Sunny Bell Flowers 134 Mummadi Reddy Ramya, Hye Ryun An, Pue Hee Park PA-177 Identification of nitrogen-use-efficiency coding and noncoding transcripts in Oryza sativa L Seon-Ju Shin, Jae-Hee Lee, Hongryul Ahn, Inuk Jung, Sun Kim, Hawk-Bin Kwon PA-178 Reconstruction of composite comparative map including ten legume species 135 Chaeyoung Lee, Jin-Hyun Kim, Joo-Seok Park, Yoram Choi, Min-Gyun Jeong, Hong-Kyu Choi PA-179 Efficient FISH analysis method using synthetic oligomers for conserved high copy repeat blocks 136 Nomar Espinosa Waminal, Remnyl Joyce Pellerin, Sung-Min Youn, Tae-Jin Yang, Hyun Hee Kim PB-01 Function of fibrillin1a, 1b and 2 genes in highlight stress in Arabidopsis 136 Inyoung Kim, Hyun Uk Kim PB-02 Characterization of trichome morphology and aphids resistance of cultivated potato and its wild species 137 Kwang-Soo Cho, Min-Kwon, Hong-Sik Won, Ji-Hong Cho, Ju-Seong Im, Jang-Gyu Choi, Young-Eun Park PB 조성우, 김학신, 최용현, 최용석, 박철수 xxxi

32 PB 조성우, 김학신, 최용현, 최용석, 박철수 PB 이원도, 강시용, 박용진, 권순욱, 이영상, 조유현 PB 이원도, 강시용, 박용진, 권순욱, 조유현 PB 이원도, 김태형, 박용진, 권순욱, 이영상, 조유현 PB 홍지화, 이영길, 강지선, 이민휘, 박영준, 김현태, 이재훤, 김동민 PB 홍지화, 이솔화, 손민식, 박영준, 김현태, 이재훤 PB 홍지화, 최경후, 김은옥, 연영자, 박영준, 김현태, 이재훤 PB 이민석, 고건희, 이정대, 유주, 이진호 PB-12 PB-13 Analysis of powdery mildew susceptible genes in Korea melon lines to develop the powdery mildew molecular marker 142 Hyung Seong Lee, Haeun Jung, Seongbin Hwang, Hyun Uk Kim Development of molecular markers for screening salt tolerant and sensitive accessions in Glycine max and Glycine soja 142 Seokhyung Lee, Gyu Tae Park, Jagadeesh Sundaramoorthy, Jeong-Dong Lee, Hak Soo Seo, Jong Tae Song PB 박기진, 박종열, 류시환, 서영호, 최재근, 남궁민, 용우식, 윤석원, 최준근 PB 김정곤, 박문웅, 성병열, 최재성, 박미자 PB 강경원, 김백준, 조영득, 강시용 PB 김정주, 신운철, 정재혁, 강위금 xxxii

33 PB 박현수, 김기영, 백만기, 조영찬, 김보경, 남정권, 고재권, 신운철, 김우재, 고종철, 김정주, 김현순, 이점호, 정종민, 강경호, 정지웅, 전재범, 이영복 PB-19 Morphological variation of accessions of Perilla Crop from north areas of China 145 Shi Jun Ma, Ju Kyong Lee PB 윤영미, 김지은, 조성우, 강천식, 정영근, 박철수 PB 김정주, 신운철, 강위금, 조현숙, 양창인, 강신구 PB-22 Difference of stachyose content between RS2 dominant allele and rs2 recessive allele in soybean seed 147 Jin-A Kim, Sung-Jin Han, Sang-Woo Choi, Dong-Hui Kang, Su-Jin Lee, Jong-Il Chung PB-23 Agronomical traits of advanced soybean line with low indigestible carbohydrates 147 Su-Jin Lee, Sung-Jin Han, Sang-Woo Choi, Dong-Hui Kang, Jin-A Kim, Jong-Il Chung PB-24 Identification of quantitative trait loci related to germinability under low oxygen condition 148 Jong-Min Jeong, Woon-chul Shin, Man-Kee Baek, Hyun-Su Park, Chun-Song Kim, Jeong-Kwon Nam, Young-Chan Cho PB-25 GWAS study for traits influencing chemical and pasting characteristics of eating quality in 96 Japonica rice germplsam 148 Jong-Min Jeong, Woon-chul Shin, Man-Kee Baek, Hyun-Su Park, Chun-Song Kim, Jeong-Kwon Nam, Young-Chan Cho PB-26 Expand phenomics matrix with non-visible spectrum (hyperspectral) in Brassica rapa subsp. chinensis (bok choy) 149 Hyo-suk Kim, Sungyul Chang, Youngchul Chung, Hyeong Seok Kim, Jae Hun Kim PB 김영진, 정종성, 황태영, 김지혜, 김원호 PB-28 PB-29 Effect of Stratification, GA3 and kinetin treatment on germinationin in Panax ginseng C.A. Meyer 150 Jung-Woo Lee, Jang-Uk Kim, Ick-Hyun Jo, Ji-Eun Hong, Kyong-Hwan Bang, Kee-Choon Park, Young-Chang Kim Effect of Stratification, GA3 and kinetin treatment on zygotic embryo development in Panax ginseng C.A. Meyer 150 Jung-Woo Lee, Jang-Uk Kim, Ick-Hyun Jo, Kyong-Hwan Bang, Kee-Choon Park, Young-Chang Kim xxxiii

34 PB-30 Resistance of strawberry cultivars to Phytophthora cactorum 151 Ye Rin Lee, Myeong Hyeon Nam, Won Suk Jang, Jin Hoon Lee, Jundae Lee PB 박석만, 고상욱, 윤수현, 박재호, 김민주, 이동훈, 최영훈 PB-32 Evaluating three priming sources to surge kenaf seed germination in non-stress and stress conditions 152 In-Sok Lee, Chan-Ho Kang, Ki-Kwon Lee PB 남정권, 박현수, 김기영, 김보경, 조영찬, 고재권, 김현순, 백만기, 김정주, 김우재, 신운철, 고종철, 이점호, 정종민, 정지웅, 강경호, 이영복 PB 남정권, 신운철, 박현수, 정종민, 김춘송, 백만기, 조영찬, 고재권, 정희채, 김보경 PB 원용재, 안억근, 장재기, 이상복, 이정희, 정국현, 김명기, 양창인, 하운구, 현웅조, 정응기, 조영찬, 오세관, 윤미라, 김보경, 김병주 PB-36 Effect of mulching on wintering and growth of autumn sown faba bean (Vicia Faba L.). 154 Min-kyu Lee, Jaihyunk Ryu, Dong-Gun Kim, Jung Min Kim, Jin-Baek Kim, Si-Yong Kang, Bo-Keun Ha, Soon-Jae Kwon PB-37 Assessment of fatty acid composition in the different part of faba bean (Vicia faba L.) cultivars 154 Dong-Gun Kim, Jaihyunk Ryu, Min-kyu Lee, Jung Min Kim, Jin-Baek Kim, Chang-Hyu Bae, Si-Yong Kang, Soon-Jae Kwon PB-38 ( 55 ) 155 김성업, 이명희, 배석복, 오은영, 오기원, 정찬식, 여운상 PB 송석보, 고지연, 우관식, 정태욱, 최명은, 김정인, 고종철, 최유미, 곽도연 PB-40 L-dopa contents of three different parts of faba bean by UPLC 156 Jung Min Kim, Jaihyunk Ryu, Min-kyu Lee, Dong-Gun Kim, Jin-Baek Kim, Si-Yong Kang, Bo-Keun Ha, Soon-Jae Kwon PB-41 A new early-heading and high-yielding forage oat cultivar "Dakyeong" 156 Tae-Il Park, Yang-Kil Kim, Hyoung-Ho Park, Ok-Kyu Han, Tae-Hwa Song, Jong-Chul Park, Jong-Ho Park, Chon-Sik Kang, Young-Jin Oh, Kyong-Ho Kim, Kyu-Hwan Choi, Ki-Hong Hong, Hyun-Seok Chae, Young-Keun Cheong, Bo-kyeong Kim xxxiv

35 PB-42 A new oat cultivar "Hi-Early" suitable in the paddy field of Korea 157 Tae-Il Park, Yang-Kil Kim, Hyoung-Ho Park, Ok-Kyu Han, Tae-Hwa Song, Jong-Chul Park, Jong-Ho Park, Chon-Sik Kang, Young-Jin Oh, Kyong-Ho Kim, Kyu-Hwan Choi, Ki-Hong Hong, Hyun-Seok Chae, Young-Keun Cheong, Bo-kyeong Kim PB-43 Biological responses and oxidative stress induced by acute and chronic gamma irradiation in Oryza sativa L. 157 Sung Min Han, Hong-Il Choi, Si-Yong Kang, Jin-Baek Kim PB 이진석, 손범영, 김정태, 배환희, 백성범, 정태욱, 김상곤, 김성국, 정건호 PB 이지윤, 조준현, 이종희, 손영보, 박노봉, 한상익, 오성환, 신동진, 송유천, 박동수, 박병명, 오인여, 남민희, 오명규 PB 구자환, 한옥규, 안종웅, 권순종 PB-47 Polymorphic SSRs for genetic diversity and molecular breeding applications in sesame (Sesamum indicum L.) 159 Sovetgul Asekova, Myung Hee Lee, Jung In Kim, Sung Up Kim, Suk Bok Bae, Un Sang Yeo, Eun Young Oh, Ki Won Oh, Do Yeon Kwak PB-48 Analysis of initial growth QTLs in rice using high-throughput plant phenotyping technology 160 Song Lim Kim, Hyenso Ji, Hongseock Lee, Eungyeong Lee, June Oh, Nyunhee Kim, Yoonkook Park, Jungmin Park, Inchan Choi, JeongHo Baek, Taek-Ryoun Kwon, Kyung-Hwan Kim PB 김경훈, 김경민, 신동진, 권유리, 현종내, 오명규 PB-50 Selection and characterization of soybean mutants with alteration in seed linolenic acid composition 161 Young Eun Jang, Min Jeong Hong, Soon-Jae Kwon, Joon-Woo Ahn, Sang Hoon Kim, Yeong Deuk Jo, Hong-Il Choi, Si-Yong Kang, Jin-Baek Kim PB 최인찬, 김송림, 이홍석, 이은경, 김년희, 백정호, 지현소, 권택윤, 김경환 PB 이상복, 현웅조, 정종민, 이정희, 서대하, 김병주, 성열규 PB 이홍석, 김송림, 이은경, 오준, 김년희, 백정호, 최인찬, 지현소, 김경환 xxxv

36 PB 김년희, 김송림, 이은경, 이홍석, 백정호, 지현소, 최인찬, 박부희, 김경환 PB-55 RGB 163 이은경, 백정호, 한희정, 김송림, 김년희, 최인찬, 정남희, 최만수, 문중경, 지현소, 김경환 PB-56 PB-57 Relationship between Agronomic Traits and Canopy Structure in determining Miscanthus giganteus Biomass Yield 164 Soo-Hyun Lim, Yeon-Ho Park, Do-Soon Kim Identification and determination of phenolic compounds in seed of Kenaf (Hibiscus cannabinus L.) mutant Cultivars 164 Jaihyunk Ryu, Soon-Jae Kwon, Yeong Deuk Jo, Jung-Min Kim, Min-Kyu Lee, Dong-Gun Kim, Joon-Woo Ahn, Jin-Baek Kim, Si-Yong Kang PB 오영진, 박태일, 김양길, 박형호, 박종철, 강천식, 한옥규, 윤건식, 홍기흥, 배정숙, 송재기, 김경호, 정영근, 송태화, 박종호 PB 오영진, 박태일, 김양길, 박형호, 박종철, 강천식, 한옥규, 윤건식, 홍기흥, 배정숙, 송재기, 김경호, 정영근, 송태화, 박종호 PB-60 Analysis of genetic variation in fruit color of Capsicum germplasm using SMRT sequencing 166 Min-Young Kang, Hyo-Bong Jeong, Ayoung Jung, Koeun Han, Moo-Chan Kang, Byoung-Cheorl Kang PB 이형운, 이준설, 정미남, 남상식, 양정욱, 김재명, 안승현, 송연상, 한선경, 황엄지 PB 김현철, 이현석, 이솔지, 강준원, 임혜민, 이위영, 장경환 PB 이현석, 김현철, 이솔지, 강준원, 임혜민, 이위영 PB 박승민, 김기용, 김지혜, 김영진, 김원호 PB 이솔지, 김현철, 이현석, 임혜민, 강준원, 이위영, 김판기 PB-66 Early maturity and white seed Perilla for edible seed Deulhyang 169 MyoungHee Lee, JiMyoung Choi, EunYoung Oh, SungUp Kim, JungIn Kim, SukBok Pae, KiWon Oh, UnSang Yeo, DoYeon Kwak xxxvi

37 PB-67 Antioxidant activities and flavonoid contents of Korean black soybean landraces 169 Da-Young Baek, Kyung Jun Lee, Myoung-Jae Shin, Gi-An Lee, Yang-Hee Cho, Kyung-Ho Ma, Yoon-Sup So, Jung-Ro Lee PB-68 Antioxidant activity in leaf extracts of Korean adzuki bean landraces 170 Kyung Jun Lee, Myoung-Jae Shin, Jung-Ro Lee, Yang-Hee Cho, Kyung-Ho Ma, Gi-An Lee PB-69 Comparison of antioxidant activities in Korean common bean landraces 170 Xiao-Han Wang, Kyung Jun Lee, Myoung-Jae Shin, Kyung-Ho Ma, Gi-An Lee, Yang-Hee Cho, Jung-Ro Lee PB-70 Comparison of tocopherol composition in Korean black soybean landraces 171 Ga-Hee Kim, Kyung Jun Lee, Myoung-Jae Shin, Gi-An Lee, Yang-Hee Cho, Kyung-Ho Ma, Yoon-Sup So, Jung-Ro Lee PB-71 Morphological diversity in Capsicum spp. germplasm 171 Sea-Hee Han, Jung-Ro Lee, Gi-An Lee, Kyung-Ho Ma PB-72 Genome-wide identification of rice collar preferred genes using meta-expression analysis (RNA-seq) and construction of the regulatory network 172 Hyo-Yong Kim, Yun-Shil Gho, Ki-Hong Jung PB-73 Lodging Tolerance and High Yield Barnyard Millet Variety Borajik 172 Jeeyeon Ko, Seokbo Song, Myeongen Choe, Kwansik Woo, Taewook Jeong, Doyeon Kwak, Kiyoung Kim, Jongcheol Ko, Inseok Oh PB-74 Evaluation of quality of perilla (Perilla frutescens var. frutescens) germplasms by using near infrared spectroscopy 173 Eun-Young Oh, Myung-Hee Lee, Jung-In Kim, Sung-Up Kim, Suk-Bok Bae, Un-Sang Yeo, Sovetgul Asekova, Ki-Won Oh, Do-Yeon Kwak PB-75 A new large grain and high yielding peanut variety "Tamsil" 173 Suk-Bok Pae, Myung-Hee Lee, Sung-Up Kim, Eun-Young Oh, Un-Sang Yeo, Ki-Won Oh, Deok-Young Song, In-Seok Oh, Yong-Chul Kim PB-76 Development of mutation populations by irradiation for breeding and functional genomics study in pepper 174 Yeong Deuk Jo, Han Sol Kang, Sang Hoon Kim, Jaihyunk Ryu, Jin-Baek Kim, Si-Yong Kang PB 현웅조, 이정희, 원용재, 정국현, 안억근, 이상복, 김명기, 하운구, 양창인, 성낙식, 조영찬, 강경호, 서대하, 김병주 PB 손지영, 박여옥, 김은경, 이영한, 최성태, 김성철, 안광환, 박두상 xxxvii

38 PB 김경훈, 윤성란, 신동진, 권유리, 김경민, 한상익, 현종내, 오명규 PB-80 PB-81 The PURPLE PERICARP trait of black rice is defined as a complementary gene interaction between Pb and Pp genes by the extended Mendelian 9:7 ratio 176 Kyung Eun Lee, Jegeun Cho, Jong Bae Kim, Sang Gu Kang Testa color might not be a factor to determine seed dormancy in wheat (Triticum aestivum L.) 176 Paulina Calderón Flores, Yong Weon Seo PB 김양길, 이미자, 박종철, 김경호, 강천식, 박종호, 한옥규, 최진경, 배정숙, 송재기, 박태일, 정영근, 김보경 PB 김양길, 박종철, 김경호, 강천식, 박종호, 한옥규, 윤건식, 배정숙, 조수현, 박태일, 정영근, 김보경 PB-84 Compositional variability in diverse maize hybrids (Literature review) 178 Mia Park, Gregory B. Tilton, Erin Bell PB-85 Growth characteristics and forage productivity of new tall fescue 178 Ki-Won Lee, Md. Atikur Rahman, Sang-Hoon Lee, Ki-Yong Kim, Hee Chung Ji, Tae Young Hwang, Gi Jun Choi PB 함태호, 권순욱, 류수노 PB 정지웅, 정종민, 강경호, 고재권, 김보경 PB 정지웅, 정종민, 김우재, 김현순, 김보경 PC-01 A study on enhancement of hydroxy fatty acid in transgenic Arabidopsis using CRISPR-Cas9- mediated gene editing 180 Mid-Eum Park, Kyeong-Ryeol Lee, Hyun Uk Kim PC-02 A new hybrid carnation Cream Magic with light yellow flower color of spray type 181 Mi-Seon Kim, Bong-Sik Yu, Jae-A Jung, Jong-Taek Park, Hak-Ki Shin xxxviii

39 PC-03 Copy number validation by using read coverage of NGS whole genome re-sequencing 181 Yang Qin, Hee-Jong Woo, Kong-Sik Shin, Myung-Ho Lim, Hyun-Suk Cho, Seong-Kon Lee PC 정용모, 진영돈, 배민지, 최시림, 홍광표, 권오창 PC-05 QTL analysis of the qbk WD, a major QTL for bakanae disease resistance in rice 182 Sais-Beul Lee, Tae-Heon Kim, Su-Min Jo, Jun-Hyun Cho, Ji-Yoon Lee, Jong-Hee Lee, Sang-Ik Han, You-Chun Song, Myung-Kyu Oh, Dong-Soo Park PC-06 A double freesia Sunny Gold with dark yellow petals 183 Youn Jung Choi, Young Ran Lee, Su Young Lee, Yun Im Kang, Hae Ryong Cho PC-07 A pink double freesia (Freesia hybrida Hort.) Love Fiction 183 Youn Jung Choi, Young Ran Lee, Su Young Lee, Yun Im Kang, Hae Ryong Cho PC 박석만, 박재호, 윤수현, 김민주 PC-09 Chalcone synthase gene targeting using the Agrobacterium tumefaciens-mediated CRISPR-Cas9 system in carrot (Daucus carota L.) 184 Eun Ju Lee, Jun Young Choi, Sun-geum Jeong, Youn-Sung Kim PC-10 CBF3 185 조신환, 안율균, 김진희, 이은수, 김정호, 김도선 PC-11 AVP1 CBF3 185 조신환, 안율균, 김진희, 이은수, 김정호, 김도선 PC 진영돈, 안동춘, 배민지, 정용모, 황주천, 최시림, 홍광표 PC-13 shed-,, 186 박은준, 양은영, 채수영, 문지혜, 박태성, 김옥례, 김대영, 이선이, 김상규 PC-14 CRISP/Cas9 Glucoraphanin 187 이상협, 황병호 PC 이진석, 손범영, 김정태, 배환희, 김상곤, 백성범 PC 박민영, 장하영, 임용표, 이정수, 박수형 xxxix

40 PC-17 DHF1, DHW1 188 류시환, 최재근, 박종열, 남궁민, 용우식, 윤석원, 박기진, 최준근 PC-18 PC-19 Assessment of genetic diversity of rice germplasm with black purple and red pericarp color using simple sequence repeats (SSR) markers 189 Jae-Ryoung Park, Won-Tae Yang, Doh-Hoon Kim, Yong-Sham Kwon The Storage stability of perilla oil affected by storage conditions and fatty acid composition 189 MyoungHee Lee, JiMyoung Choi, EunYoung Oh, SungUp Kim, JungIn Kim, SukBok Pae, KiWon Oh, UnSang Yeo, DoYeon Kwak PC-20 Modification of seed colors via CRISPR/Cas9-targeted knockout of LDOX gene in rice 190 Shahina Akter, Hyo-Ju Lee, Ye Rim Lee, Yeo Jin Jung, Jong Hee Kim, Yong-Gu Cho, Kwon-Kyoo Kang, Yu-Jin Jung PC-21 High-frequency genome editing with the CRISPR/Cas9 system in rice 190 Hyo-Ju Lee, Sangsu Bae, Geung-Joo Lee, Pil Joon Seo, Dong Hyun Kim, Jae Uk Lee, Yong-Gu Cho, Kwon Kyoo Kang, Yu Jin Jung PC 김남훈, 김혜식, 신명주, 이현오, 최범순, 유의수 PC-23 Development of wheat transformation by biolastic approach in a Korean wheat cultivar Keumkang 191 Jin Sun Kim, Su-Jin Choi, You-Ran Jang, Sun-Hyung Lim, Jong-Yeol Lee PC-24 AVMP (Assembly Verification using Mate Pair) 192 박미소, 김남훈, 이승욱, 유의수, 강상호, 최범순 PC-25 PC-26 PC-27 Early maturing rice Variety, Gawaji 1, adaptable to climatic change and double cropping system in Gyeonggi-Do, northern area of South Korea 192 Ji-Hye Lee, Jung-Hee Jang, Byoung-Rourl Choi, Tae-Jin Won Overexpression of the Oryza sativa glutathione synthetase improved stress tolerance and productivity in transgenic rice plants under field conditions Seong-Im Park, Young-Saeng Kim, Jin-Ju Kim, Il-Sup Kim, Ho-Sung Yoon Overexpression of a novel RING-type E3 ubiquitin ligase gene induces the coiled branch (cbr) mutant in Arabidopsis Gyu Tae Park, Jagadeesh Sundaramoorthy, Seokhyung Lee, Jeong-Dong Lee, Hak Soo Seo, Jong Tae Song xl

41 Concurrent session OF-01 SP-LL-37, human antimicrobial peptide, enhances disease resistance in transgenic rice 195 In Hye Lee, Yu-Jin Jung, Yong Gu Cho, Ill Sup Nou, Md. Amdadul Huq, Franz Marielle Nogoy, Kwon-Kyoo Kang OF-02 Optimization of crop productivity in tomato by manipulating genetic variations in florigen related genes 195 Soon Ju Park, Sebastian Soyk, Min-Sung Kang, Zachary B Lippman OG-01 Utilizing multi-omic information in rice breeding: experience from new trials 199 Kyu-Won Kim, Qiang He, Win Htet Oo, Ji-Min Yoo, Phitaktansakul Rungnapa, Dong-Hee Kim, Jung-Rye Nam, Sang-Ho Chu, Wei-Guo Zhao, Sang-Won Park, Il-Pyung Ahn, Yong-Soo Choi, Yoo-Hyun Cho, Young-Sang Lee, Jung-Kyung Moon, Yong-Jin Park OG-02 Construction of core collection using single nucleotide polymorphism analysis in cultivated soybeans (Glycine max L. Merr) and wild soybeans (Glycine soja Siebold & Zucc.) germplasm. 199 Man-Soo Choi OG-03 GWAS of primary agronomic characters in Capsicum core collection 200 Jin-Kyung Kwon, Hea-Young Lee, Koeun Han, Na Young Ro, On-Sook Hur, Ho-Cheol Go, Byoung-Cheorl Kang OG-04 Soybean nested association mapping(nam) population for novel gene/qtl mass screening 200 Sung-Taeg Kang, Ju-Seok Lee, Jung-Kyung Moon, Soo-Kwon Park, Man-Soo Choi, Soon-Chun Jeong, Namshin Kim, Bo-Keun Ha OH 박용주, 이문호 OH 박은준, 양은영, 채수영, 문지혜, 박태성, 김옥례, 김대영, 이선이, 김상규 xli

42 OH 최강준 OH 박종인, 김회택, 노일섭 xlii

43 Plenary Session

44

45 Plenary Session

46 SYMP-01 Bing Yang Engineered nucleases represent a group of potent enzymes to induce DNA double strand breaks (DSBs) and subsequently precise genetic alteration in target genomes. Modified CRISPR (clustered regularly interspaced short palindromic repeats) systems, comprising Cas9 nucleases and single guide RNAs (sgrnas), and TALENs (TAL effector nucleases) have emerged as potent biotechnological tools for both basic and applied research. The most promising utilization of both Cas9/sgRNA and TALENs is for targeted genome editing, precise genetic alterations within any genome of interest, as demonstrated in a plethora of organisms including several crop plants. My presentation describes development and application of these two technologies to generate heritable genome modifications in rice and maize. TALENs were employed to generate stable, heritable mutations in dozens of rice genes and maize glossy2 gene. The frequency of mutagenesis varied from species and constructs targeting different genes. The highest rate in rice reached about 65% of transgenic lines carry desired mutations, while construct targeting the maize glossy2 gene produced mutant lines from the maize genotype Hi-II at a frequency of about 10%. Phenotypic changes associated with mutated genes were observed in some mutant lines in rice and maize. We also modified the Cas9/sgRNA system suitable for targeted gene mutagenesis in rice and maize, respectively. The two systems have been successfully applied to rice and maize for targeted mutagenesis of many genes. Transgenic lines of T0 generation carrying site-specific mutations were produced at frequency as high as 100% in rice and 80% in maize. Our results demonstrate that TALENs and Cas9/sgRNA are effective toolboxes for genome editing in rice and maize, empowering the discovery of gene function and the trait improvement. Corresponding Author: byang@iastate.edu 4

47 Associate Professor Department of Genetics, Development and Cell Biology Iowa State University Ames, IA USA Research interests - Genome editing technology development and application in plants, -TALENs, CRISPR; - Microbe/host plant interaction, - basic understanding of molecular mechanism underlying plant disease susceptibility and resistance mediated through bacterial type III effectors; - Engineering plant disease resistance through genome editing Education May Ph.D. in Plant Pathology Kansas State University June M.S. in Agriculture Southwest Forestry University, Chin June B.S. in Agriculture Southwest Forestry University, China Professional experiences(starting with the most recent one) Associate Professor Iowa State University Assistant Professor Iowa State University Research Assist. Prof. Kansas State University Postdoc Res. Associate Kansas State University Graduate Res. Assistant Kansas State University Research Assistant Kunming Institute of Zoology, CAS Selected publications Si Nian Char,Anjanasree K. Neelakandan, Hartinio Nahampun, Bronwyn Frame, Marcy Main, Martin H. Spalding, Philip W. Becraft, Blake C. Meyers, Virginia Walbot, *Kan Wang, *Bing Yang An Agrobacterium delivered CRISPR/Cas9 system for high frequency targeted mutagenesis in maize. Plant Biotechnology Journal15(2): Si Nian Char, Erica Unger-Wallace, Bronwyn Frame, Sarah A. Briggs, Marcy Main, Martin H. Spalding, Erik Vollbrecht, Kan Wang and * Bing Yang 2015 Heritable site-specific gene mutagenesis using TALENs in maize. Plant Biotechnology Journal 13(7): Huanbin Zhou, Bo Liu, Donald Weeks, Marty Spalding and * Bing Yang 2014 Large chromosomal deletions and heritable small genetic changes induced by CRISPR/Cas9 in rice. Nucleic Acids Research42, WenZhi Jiang, Bing Yang and *Donald Weeks Efficient CRISPR/Cas9-mediated gene editing in Arabidopsis thaliana and inheritance of modified genes in T2 and T3 generations. PloS One 9:e Li, T., Liu, B. Spalding, H.M., Weeks, D. and * Yang, B High efficiency TALEN-based gene editing produces disease resistant rice. Nature Biotechnology 30, Li, T., Huang, S. (co-first author), Zhao, X., Wright, D., Carpenter, S., Spalding, M.H., Weeks, P.D. and * Yang, B The modularly assembled designer TAL effector nucleases for targeted gene knockout and gene replacement in eukaryotes. Nucleic Acids Research, 39, Zhiyuan Ji, Chonghui Ji, Bo Liu, Lifang Zou, *Gongyou Chen, *Bing Yang2016 Interfering TAL effectors of Xanthomonas oryzae neutralize R-gene-mediated plant disease resistance. Nature Communications 7: Sheng Huang, Ginny Antony, Ting Li, Bo Liu, Ken Obasa, *Bing Yang, *Frank F White2016The broad recessive resistance gene xa5 of rice is a virulence effector-dependent quantitative trait for bacterial blight. Plant Journal 86(2): Junhui Zhou, Zhao Peng, Juying Long, Davide Sosso, Bo Liu, Joon-Seob Eom, Sheng Huang, Sanzhen Liu, Casiana Vera Cruz, Wolf B. Frommer, Frank F. White, * Bing Yang Gene targeting by the TAL effector PthXo2 reveals cryptic resistance gene for bacterial blight of rice. Plant Journal 82(4): Antony, G., Zhou, J. (co-first author), Huang, S., Li, T., Liu, B., White, F.F., and * Yang, B Rice xa13 recessive resistance to bacterial blight is defeated by induction of the disease susceptibility gene Os-11N3. Plant Cell 22:

48 SYMP-02 Sanwen Huang The majority crop today is domesticated from their wild ancestors. Cucurbits were domesticated from their wild ancestors that had extremely bitter fruits. Cucurbitacins are triterpenoids that confer a bitter taste in cucurbits such as cucumber, melon, watermelon, squash, and pumpkin. With genomics and biochemistry, we identified nine cucumber genes in the pathway for biosynthesis of cucurbitacin C and elucidated four catalytic steps. We discovered transcription factors Bl (Bitter leaf) and Bt (Bitter fruit) that regulate this pathway in leaves and fruits, respectively. Traces in genomic signatures indicated that selection imposed on Bt during domestication led to derivation of nonbitter cucurbits from their bitter ancestors. Tomato serves as an important resource of vitamins, minerals and other nutrients in daily diet, however, the mordern commercial tomato suffers from the deterioration in flavor. To understand and ultimately correct this deficiency, we quantified flavor associated chemicals in 398 modern, heirloom, and wild accessions. A subset of these accessions was evaluated in consumer panels, identifying the chemicals that made the most important contributions to flavor and consumer liking. We found that modern commercial varieties contain significantly lower amounts of many of these important flavor chemicals than older varieties. Whole-genome sequencing and a genome-wide association study permitted identification of genetic loci that affect most of the target flavor chemicals, including sugars, acids, and volatiles. Together, these results provide an understanding of the flavor deficiencies in modern commercial varieties and the information necessary for the recovery of good flavor through molecular breeding. Corresponding Author: huangsanwen@caas.cn 6

49 Principal Investigator Deputy Director General Agricultural Genomics Institute, Chinese Academy of Agricultural Sciences (CAAS) Shenzhen , China Phone: Research interests - Decoding vegetable genomes of cucumber, potato, tomato, etc. - Developing variation maps of vegetables - Uncovering the genetic basis of important biological processes Education Ph.D. in Plant Breeding Wageningen University M.S. in Plant Science China Agricultural University B.S. in Horticulture China Agricultural University Professional experiences(starting with the most recent one) Principal Investigator Agricultural Genomics Institute, Chinese Academy of Agricultural Sciences (CAAS) Principal Investigator Institute of Vegetables and Flowers, CAAS Research Associate Institute of Vegetables and Flowers, CAAS Selected publications Denise Tieman, Guangtao Zhu,, Matias Kirst, Sanwen Huang, Harry Klee. (2017) A chemical genetic roadmap to improved tomato flavor. Science, 355: (Corresponding authors). Yuan Zhou, Yongshuo Ma, Jianguo Zeng, Lixin Duan, Xiaofen Xue,, Yi Shang, Sanwen Huang (2016)Convergence and divergence of bitterness biosynthesis and regulation in Cucurbitaceae. Nature Plants, 16183:1-8 (Corresponding authors). Shenhao Wang, Xueyong Yang,, Sanwen Huang(2015) A rare SNP identified a TCP transcription factor essential for tendril development in cucumber. Molecular Plant, 8: (Corresponding authors). Zhonghua Zhang, Linyong Mao, Huiming Chen, Fengjiao Bu, Guangcun Li,, Sanwen Huang (2015) Genome-wide mapping of structural variations reveals a copy number variant that determines reproductive morphology in cucumber, Plant Cell, 27: (Corresponding authors). Yi Shang, Yongshuo Ma, Yuan Zhou, Huimin Zhang,, Sanwen Huang (2014) Biosynthesis, regulation and domestication of bitterness in cucumber, Science,346: (Corresponding authors). Tao Lin, Guangtao Zhu, Junhong Zhang, Xiangyang Xu, Qinghui Yu, Zheng Zheng,, Jingfu Li, Zhibiao Ye, Yongchen Du, Sanwen Huang (2014) Genomic analyses provide insights into the history of tomato breeding, Nature Genetics,46: (Corresponding authors). Jianjian Qi, Xin Liu, Di Shen, Han Miao, Bingyan Xie, Xixiang Li,,Zhonghua Zhang, Sanwen Huang (2013) A genomic variation map provides insights into the genetic basis of cucumber domestication and diversity,nature Genetics, 45: (Corresponding authors). The Potato Genome Sequencing Consortium (2011) Genome sequence and analysis of the tuber croppotato, Nature,475: (Corresponding authors). Sanwen Huang, Ruiqiang Li, Zhonghua Zhang, Li Li, Xingfang Gu, Wei Fan, William J Lucas,, Jun Wang, Yongchen Du, Songgang Li (2009) The genome of the cucumber (Cucumis sativus L.), Nature Genetics, 41: (Co-first authors/ Corresponding authors). 7

50 SYMP-03 Kristin Bilyeu Achieving appropriate maturity in a target environment is essential to maximizing crop yield potential. In soybean [Glycine max (L.) Merr.], the time to maturity is largely dependent on developmental response to dark periods. Once the critical photoperiod is reached, flowering is initiated and reproductive development proceeds. Therefore, soybean adaptation has been attributed to genetic changes and natural or artificial selection to optimize plant development in specific, narrow ranges of latitude. In the United States (US), these regions have been classified into maturity groups (MG). Growing soybean lines not adapted to a particular environment typically results in poor growth and significant yield reductions. The objective of this study was to develop a molecular model for soybean maturity based on the alleles underlying the major maturity loci: E1, E2, and E3. We examined allelic variation and diversity of the E maturity genes in a large collection of landraces, North American ancestors, Chinese cultivars, North American cultivars or expired Plant Variety Protection lines, and private-company lines. The E gene status of accessions in the USDA Soybean Germplasm Collection with SoySNP50K Beadchip data was also predicted. We determined the E allelic combinations needed to adapt soybean to different MGs in the US and discovered a strong signal of selection for E genotypes released in the US. The E gene maturity model proposed will enable plant breeders to more effectively transfer traits into different MGs and increase the overall efficiency of targeted breeding for specific MGs. Corresponding Author: kristin.bilyeu@ars.usda.gov 8

51 Research Molecular Biologist Plant Genetics Research Unit Agricultural Research Service-USDA 110 Waters Hall University of Missouri Columbia, Missouri USA Phone: Research interests - Molecular-genetic analysis and characterization of soybean seed composition traits to improve oil and meal functionality - Optimizing allele combinations to maximize soybean yield potential in different environments - Development of high oleic acid and low linolenic acid soybean germplasm - Increasing palatability, nutrition, and functionality of soy foods Education Dec. Ph.D. in Cell and Molecular Biology Baylor College of Medicine May B.S. in Biochemistry University of Missouri. Professional experiences (starting with the most recent one) GS-15 USDA/ARS GS-14 USDA/ARS GS-13 USDA/ARS GS-12 USDA/ARS Postdoctoral fellow USDA/ARS and University of Missouri Selected publications Langewisch T, Lenis J, Jiang G-L, Wang D, Pantalone V, Bilyeu K (2017) The development and use of a molecular model for soybean maturity groups. BMC Plant Biology 17 (1):91 Bilyeu KD, Wiebold WJ (2016) Environmental Stability of Seed Carbohydrate Profiles in Soybeans Containing Different Alleles of the Raffinose Synthase 2 (RS2) Gene. Journal of Agricultural and Food Chemistry 64 (5): Langewisch T, Zhang H, Vincent R, Joshi T, Xu D, Bilyeu K (2014) Major Soybean Maturity Gene Haplotypes Revealed by SNPViz Analysis of 72 Sequenced Soybean Genomes. PLoS ONE 9 (4):e94150 Pham AT, Shannon JG, Bilyeu KD (2012) Combinations of mutant FAD2 and FAD3 genes to produce high oleic acid and low linolenic acid soybean oil. Theor Appl Genet 125 (3): Pham A-T, Lee J-D, Shannon J, Bilyeu K (2011) A novel FAD2-1A allele in a soybean plant introduction offers an alternate means to produce soybean seed oil with 85% oleic acid content. TAG Theoretical and Applied Genetics 123 (5): Gillman J, Tetlow A, Lee J-D, Shannon JG, Bilyeu K (2011) Loss-of-function mutations affecting a specific Glycine max R2R3 MYB transcription factor result in brown hilum and brown seed coats. BMC Plant Biology 11 (1):155 Pham A-T, Lee J-D, Shannon JG, Bilyeu K (2010) Mutant alleles of FAD2-1A and FAD2-1B combine to produce soybeans with the high oleic acid seed oil trait. BMC Plant Biology 10 (1):195 Dierking EC, Bilyeu KD (2008) Association of a soybean raffinose synthase gene with low raffinose and stachyose seed phenotype. The Plant Genome 1 (2):

52 SYMP-04 Kee Hoon Sohn Plant innate immunity relies on two layers of pathogen detection. Cell surface-localized pattern recognition receptors detect pathogen-associated molecular patterns (PAMPs) of invading microorganisms and activate PAMP-triggered immunity (PTI). Successful pathogens must circumvent PTI to colonize plants, and many bacterial pathogens use type III secretion (T3S) to deliver effectors that suppress PTI into plant cells. Effectors can be detected directly or indirectly by plant disease resistance (R) proteins, which then activate effector-triggered immunity (ETI) generally together with a hypersensitive response (HR) of the infected tissue. Plant pathogenic bacteria, Pseudomonas syringae and Erwinia amylovora, translocate a type III secretion-dependent effector protein, AvrRpt2, in host plant cells. AvrRpt2 is a cysteine protease and cleaves its host target protein RIN4. AvrRpt2-directed cleavage of RIN4 is recognized by CC-NB-LRR type immune receptors RPS2 and MR5 in Arabidopsis and Malus, respectively. Interestingly, RPS2 and MR5, although both recognize AvrRpt2, do not share significant sequence homology. We found that RPS2 but not MR5 shows autoactivity as shown by a rapid programmed cell death when transiently overexpressed in Nicotiana benthamiana leaf cells. Thus, we hypothesized that the mechanisms by which RPS2 and MR5 are activated by AvrRpt2-directed cleavage of RIN4 differ from each other. Further mechanistic details of AvrRpt2-triggered activation of MR5 will be presented. Corresponding Author: khsohn@postech.ac.kr 10

53 Assistant Professor Department of Life Sciences, Postech, 77 Cheongam-ro, Nam-gu, Pohang, 37673, Republic of Korea Phone: Research interests - Molecular mechanisms of plant disease resistance and susceptibility - Engineering and deployment of plant immune receptors to develop disease resistant crops Education 2009 Ph.D. in Biological Science University of East Anglia/The Sainsbury Laboratory (UK) 2003 M.S. in Plant Pathology Korea University 2001 B.S. in Agricultural Biology Korea University Professional experiences(starting with the most recent one) 2015-present Assistant Professor Postech 2015-present Adjunct Associate Professor Massey University (NZ) Lecturer/Bioprotection Research Fellow Massey University (NZ) Senior Research Associate The Sainsbury Laboratory (UK) Selected publications Jayaraman, J., Choi, S., Prokchorchik, M., Choi, D.S., Spiandore, A., Rikkerink, E.H., Templeton, M.D., Segonzac, C. and Sohn, K.H. (2017) A bacterial acetyltransferase triggers immunity in Arabidopsis thaliana independent of hypersensitive response. Scientific Reports (in press) Zhang, X., Bernoux, M., Bentham, A.R., Newman, T.E., Ve, T., Casey, L.W., Raaymakers, T.M., Hu, J., Croll, T.I., Schreiber, K.J., Staskawicz, B.J., Anderson, P.A., Sohn, K.H., Williams, S.J., Dodds, P.N. and Kobe, B. (2017) Multiple functional self-association interfaces in plant TIR domains. Proceedings of the National Academy of Sciences of the United States of America 114, E2046-E2052 Sarris, P.F., Duxbury, Z., Huh, S.U., Ma, Y., Segonzac, C., Sklenar, J., Derbyshire, P., Cevik, V., Rallapalli, G., Saucet, S.B., Wirthmueller, L., Menke, F.L.H., Sohn, K.H. and Jones, J.D.G. (2015) A plant immune receptor complex is activated by bacterial effectors that target WRKY transcription factors. Cell 161, Saucet, S.B., Ma, Y., Sarris, P.F., Sohn, K.H. and Jones J.D.G. (2015) Two linked pairs of TNL resistance genes function independently to recognize bacterial effector AvrRps4 in Arabidopsis. Nature Communications 6:6338 Sohn, K.H., Segonzac, C., Rallapalli, G., Sarris, P.F., Woo, J.Y., Williams, S.J., Newman, T.E., Paek, K.H., Kobe, B. and Jones, J.D.G. (2014) The Nuclear Immune Receptor RPS4 is Required for RRS1 SLH1 -Dependent Constitutive Defense Activation in Arabidopsis thaliana. PLOS Genetics 10, e Williams, S.J., Sohn, K.H., Wan, L., Bernoux, M., Sarris, P.F., Segonzac, C., Ve, T., Ma, Y., Saucet, S.B., Ericsson, D.J., Casey, L.W., Lonhienne, T., Winzor, D.J., Zhang, X., Coerdt, A., Parker, J.E., Dodds, P.N., Kobe, B. and Jones, J.D.G. (2014) Structural Basis for Assembly and Function of a Heterodimeric Plant Immune Receptor. Science 344, Sohn, K.H., Hughes, R., Piquerez, S., Jones, JD and Banfield, M. (2012) Identification of crystal structure and functional analysis of bacterial effector AvrRps4. Proceedings of the National Academy of Sciences109,

54 SYMP-05 Susan B. Altenbach Many proteins that contribute to the unique viscoelastic properties of wheat flour also trigger human health problems including food allergies, celiac disease and other wheat sensitivities. To determine whether it is possible to reduce the immunogenic potential of wheat flour without impacting its functional properties, RNA interference was used to silence two complex groups of gluten protein genes in transgenic wheat. The omega-5 gliadins trigger the serious food allergy wheat-dependent exercise-induced anaphylaxis (WDEIA) while the omega-1,2 gliadins contain immunodominant epitopes for celiac disease. Transgenic lines were selected in which all target proteins were significantly reduced in flour with few changes in other flour proteins. End-use quality was assessed in omega-5 gliadin suppressed lines using mixing and baking studies and the allergenic potential of the flour was evaluated by 2-dimensional immunoblot analysis using sera from a collection of WDEIA patients. The data demonstrate that elimination of omega-5 gliadins results in flour with decreased allergenic potential and improved end-use quality. Similar analyses are being conducted with omega-1,2 gliadin suppressed lines. While RNA interference proved to be an effective strategy to reduce the immunogenic potential of wheat flour, the resultant plants are transgenic and unlikely to reach the marketplace because of consumer acceptance issues. Future studies will employ genome editing to develop wheat with reduced immunogenic potential that can be rapidly deployed in breeding programs. Corresponding Author: susan.altenbach@ars.usda.gov 12

55 Research Biologist USDA-ARS Western Regional Research Center 800 Buchanan Street Albany, California USA Phone: Research interests - Molecular basis of wheat end-use quality and immunogenic potential - Proteomic analysis of the effects of environment on wheat flour protein composition and end-use quality - Biotechnology approaches to improve end-use quality and reduce immunogenic potential of wheat flour Education 1983 Ph.D. in Biology University of California, San Diego 1977 B.S. in Zoology University of Wisconsin, Madison Professional experiences 1992-present Research Biologist USDA-ARS Western Regional Research Center, Albany, CA, USA Senior Scientist The Plant Cell Research Institute, Inc., Dublin, CA, USA Postdoc &Staff Scientist ARCO Plant Cell Research Institute, Dublin, CA, USA. Selected publications Lee, J.Y., Beom, H.-R., Altenbach, S.B., Lim, S.-., Kim, Y-T., Kang, C-S., Yoon, U-H., Gupta, R, Kim, S.T., Ahn, S.-N., Kim, Y-M. Comprehensive identification of LMW-GS genes and their protein products in a common wheat variety. Functional and Integrative Genomics 16: Altenbach, S.B., Tanaka, C.K., Whitehand, L.C., Vensel, W.H. Effects of post-anthesis fertilizer on the protein composition of the gluten polymer in a US bread wheat. Journal of Cereal Science 68: Altenbach, S.B., Tanaka, C.K., Pineau, F., Lupi, R., Drouet, M, Beaudoin, E., Morisset, M., Denery-Papini, S. Assessment of the allergenic potential of transgenic wheat (Triticum aestivum) with reduced levels of 5-gliadins, the major sensitizing allergen in wheat-dependent exercise-induced anaphylaxis. Journal of Agricultural and Food Chemistry 63: Huebener, S., Tanaka, C.K., Uhde, M., Zone, J.J., Vensel, W.H., Kasarda, D.D., Beams, L, Briani, C., Green P.H.R., Altenbach, S.B., Alaedini, A. Specific nongluten proteins of wheat are novel target antigens in celiac disease humoral response. Journal of Proteome Research 14: Altenbach, S.B., Tanaka, C.K., Seabourn B.W. Silencing of omega-5 gliadins in transgenic wheat eliminates a major source of environmental variability and improves dough mixing properties of flour. BMC Plant Biology 14: Hurkman, W.J., Tanaka, C.K., Vensel, W.H., Thilmony, R., Altenbach, S.B. Comparative proteomic analysis of the effect of temperature and fertilizer on gliadin and glutenin accumulation in the developing endosperm and flour from Triticum aestivum L. cv. Butte 86. Proteome Science 11: Altenbach, S.B. New insights into the effects of high temperature, drought and post-anthesis fertilizer on wheat grain development. Journal of Cereal Science 56: Altenbach, S.B., Tanaka, C.K., Hurkman, W.J., Whitehand, L.C., Vensel, W.H., and Dupont, F.M. Differential effects of a post-anthesis fertilizer regimen on the wheat flour proteome determined by quantitative 2-DE. Proteome Science 9: DuPont, F.M., Vensel, W.H., Tanaka, C.K., Hurkman, W.J., Altenbach, S.B. Deciphering the complexities of the wheat flour proteome using quantitative two-dimensional electrophoresis, three proteases and tandem mass spectrometry, Proteome Science, 9:

56 SYMP-06 David Yeh China is the second largest seed market in the world with a market value of $7.5bn (ex-company price). Agriculture modernization remains high priority for China and seed industry development plays a key role in agriculture supply-side structural reform. Since 2015, China has revised its Seed Law and released a series of implementation regulations to promote the reform of marketization and support domestic industry development. The Chinese government is working to create favorable environment for innovation in the seed sector by strengthening IPR and encouraging the private sector to be more involved in variety breeding. Biotechnology development has been written into China National Strategy. China will pursue the commercialization of GM corn to address market demand and food security and will continue to enhance GMO administration, stewardship and public education. Currently, several crops are in R&D process such as herbicide tolerant (HT) /disease resistant (DR)/insect resistant (IR) rice, IR/HT corn, salinity tolerant (ST)/HT/DR soybean, DR/HT OSR, HT/drought tolerant (DT) wheat and DT/HT/DR cotton, etc. Corresponding Author: David.yeh@bayer.com 14

57 Head Market Acceptance APAC &Seeds Greater China, Bayer CropScience (China) Co., Ltd. Bayer Center, No. 27, Dongsanhuan North Road, Chaoyang District, China Phone: Education MBA degree Vlerick Leuven Gent Management school/ Peking University joint program M.S. Biological Systems Engineering and Texas A&M University Biotechnology &Certification of Business Professional experiences 2015 Head of Market Acceptance APAC Bayer CropScience China 2012 Head of Seeds Greater China Bayer CropScience China GMO regulatory &market acceptance Bayer CropScience China for S. East Asia China Registration Manager Bayer CropScience China 15

58 SYMP-07 Ju-Kon Kim Genome-editing is going to generate new crop varieties with desirable traits that can satisfy the various demands for global agriculture. As one of the new plant breeding techniques, genome-editing allows plant breeding without introducing a transgene, and this has led to new challenges for the regulation and social acceptance of genome-edited crops. This modern technology can produce novel plants that are similar or identical to those generated by conventional breeding techniques, thus creating indistinct boundaries with regards to genetically modified organism (GMO) regulations. Therefore an appropriate regulatory response is required towards the social acceptance of genome-edited crops. In this symposium, I review the recent development of genome-editing of crops and propose a concept of appropriate regulatory models by unraveling the indistinct boundaries. Corresponding Author: jukon@snu.ac.kr 16

59 서울대학교국제농업기술대학원교수 / 학과장종자생명과학연구소장강원도평창군대화면평창대로 jukon@snu.ac.kr 교육및연구경력 서울대학교농화학과학사 한국과학기술원 (KAIST) 생물공학과석사 미국코넬대학교식물분자생물학박사 명지대학교생명과학정보학부조교수 / 부교수 / 교수 한국분자세포생물학회, 편집위원 한국응용생명화학회간사장, 부편집위원장, 편집위원장 한국연구재단 RB (Review Board, 전문위원 ) 한국응용생명화학회장 2008-현재 한국식물생명공학회부회장, 편집위원 2011-현재 차세대바이오그린21사업 / 전문가위원회위원장 2013-현재 서울대학교국제농업기술대학원교수 / 학과장종자생명과학연구소장 2017-현재 한국과학기술단체총연합회부회장 / 학술진흥위원회농수산분과위원장 최근 3 년간논문게재실적 Jeong, J.S., Jung, C., Seo, J. S., Kim, J.-K. and Chua, N.-H. (2017) The deubiquitinating enzymes UBP12 and UBP13 positively regulate MYC2 levels in jasmonate responses. Plant Cell, in press (co-corresponding author) Lee, D.-K., Kim, Y. S. and Kim, J.-K. (2017) Determination of the optimal condition for ethylmethane sulfonate-mediated mutagenesis in a Korean commercial rice, Japonica cv. Dongjin. Applied Biological Chemistry, in press (co-corresponding author). Jung, H., Chung, P.J., Park, S.-H., Redillas, M.C.F.R., Kim, Y. S., Suh, J.-W. and Kim, J.-K. (2017) Overexpression of OsERF48 causes regulation of OsCML16, a calmodulin-like protein gene that enhances root growth and drought tolerance. Plant Biotechnology Journal, in press (co-corresponding author). Lee, D.-K., Chung, P.J., Jeong, J.S., Jang, G., Jung, H., Bang, S.W., Kim, Y. S., Ha, S.-H., Choi, Y. D. and Kim, J.-K. (2017) The rice OsNAC6 transcription factor orchestrates multiple molecular mechanisms involving root structural adaptions and nicotianamine biosynthesis for drought tolerance. Plant Biotechnology Journal 15: (corresponding author). Yoon, S., Lee, D.-K., Yu, I. J., Kim, Y.S., Choi, Y. D., and Kim, J.-K. (2017) Overexpression of the OsbZIP66 transcription factor enhances drought tolerance of rice plants. Plant Biotechnology Reports 11: (corresponding author). Lee, D.-K., Yoon, S., Kim, Y. S. and Kim, J.-K. (2017) Rice OsERF71-mediated root modification affects shoot drought tolerance. Plant Signaling & Behavior 12:1 (Corresponding author) Lee, D.-K., Jung, H., Jang, G., Jeong J.S., Kim, Y. S., Ha, S.-H., Choi, Y. D. and Kim, J.-K. (2016) Overexpression of the OsERF71 transcription factor alters rice root structure and drought resistance. Plant Physiology 172: (corresponding author). Lee, D.-K., Park, S.-H., Seong, S.-Y., Kim, Y. S., Jung, H., Choi, Y. D. and Kim, J.-K. (2016) Production of insect-resistant transgenic rice plants for use in practical agriculture. Plant Biotechnology Reports 10: (corresponding author). Chung, P.J., Jung, H., Ha, S.-H., Choi, Y. D. and Kim, J.-K. (2016) Transcriptome profiling of drought responsive noncoding RNAs and their target genes in rice. BMC Genomics 17: (corresponding author). Lee, D.-K., Jang, G., Chung, P., Jeong, J. S., Kim, Y. S., Bang, S. W., Jung, H., Choi, Y. D. and Kim, J.-K. (2015) The OsNF-YA7 confers drought stress tolerance of rice in an ABA independent manner. Plant Science 241: (Corresponding author) Park, S.-H., Jeong, J.S., Choi, Y.D. and Kim, J.-K. (2015) Characterization of the rice RbcS3 promoter and its transit peptide for use in chloroplast targeted expression. Plant Biotechnology Reports 9: (Corresponding author) Jung, H., Lee, D.-K., Choi, Y. D. and Kim, J.-K. (2015) OsIAA6, a member of the rice Aux/IAA gene family, is involved in drought tolerance and tiller outgrowth. Plant Science 236: (Corresponding author) Bang, S.-W., Park, S.-H., Kim, Y. S., Choi, Y. D. and Kim, J.-K. (2015) The activities of four constitutively expressed promoters in single-copy transgenic rice plants for two homozygous generations. Planta 241: (Corresponding author) Park, S.-H., Jeong, J.S., Lee, K.H., Kim, Y.S. Choi, Y.D. and Kim, J.-K. (2015) OsbZIP23 and OsbZIP45, members of the rice basic leucine zipper transcription factor family, are involved in drought tolerance. Plant Biotechnology Reports 9: (Corresponding author) 17

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63 OA-01 Sung-Ryul Kim, Joie Ramos, Sherry Lou Hechanova, G.D. Prahalada, Jeom-Sig Lee and Kshirod K. Jena Rice (Oryza sativa L.) is one of the major crops and feed more than half of the world s population. Especially people in most of Asian countries have been eating rice as a staple food, and in current situation rice consumption is rapidly growing in African countries. The International Rice Research Institute (IRRI) has been doing important roles in overall rice sciences including finding/sharing new valuable traits of rice, breeding/distribution of new rice varieties, sharing genetic resources, gender research and training of rice scientists/breeders to reduce poverty and to secure stable rice production, especially in South East and South Asian countries expanding to East African countries, since foundation of IRRI in In the presentation, the current breeding target traits/genes of IRRI with molecular breeding strategy will be briefly introduced. The presentation will be more focused on marker-assisted breeding of rice yield-enhancing genes such as Gn1a and OsSPL14 for improvement of indica rice cultivars and revealing of optimal alleles combinations of five major heading genes (Hd1, Hd2/OsPRR37, Hd3a, Hd4/Ghd7 and Hd5/DTH8) for the adaption of Korean varieties in the tropical regions. Furthermore utilization of high-throughput and high-density SNP genotyping system, whole genome sequencing, and CRISPR/Cas9 genome editing techniques for molecular breeding will be discussed. Corresponding Author: s.r.kim@irri.org OA-02 Kiyoon Kang, Sung-Hwan Cho and Nam-Chon Paek * As an important agronomic trait, rice leaf rolling has attracted much attention from plant biologists and breeders because moderate leaf rolling increases photosynthetic competence and hence raises grain yield. However, the relevant molecular mechanism remains unclear. Rolled fine striped (RFS), a key gene controlling rice leaf rolling, chloroplast development and ROS scavenging, was isolated and characterized. Map-based cloning revealed that rfs-1 mutant harbors CHR4/Mi-2 chromatin remodeling factor belonged to the SNF2-ATP-dependent chromatin remodeling family. The rfs-2 T-DNA insertion mutants under different genetic background mimicked the rfs-1 phenotype, exhibiting extremely incurved leaves phenotype due to defective development of vascular cells on the adaxial side. RFS was expressed in various tissues and accumulated mainly in the vascular cells throughout leaf development. Furthermore, RFS deficiency resulted in cell death phenotype, that was caused by generating more ROS in rfs-2 mutants compared with wild-type. Quantitative PCR experiments showed that expression of five ROS-scavenging genes (CATC, APX8, putative copper/zinc superoxide dismutase, putative superoxide dismutase and Prx IIE2) was decreased in the rfs-2 mutant. Further analysis by western-blot and chromatin immunoprecipitation (ChIP) demonstrated that RFS had reduced levels of H3K9me2, H3K4me3 and H3K27me3 at five ROS-scavenging genes. Loss-of-function in RFS also led to developmental defect including pollen development, grain filling and root development. Our results suggest that RFS is involved in development processes and play an important role in ROS scavenging to modulate ROS homeostasis. *Corresponding Author: ncpaek@snu.ac.kr 21

64 OA-03 Jinhuan Wei 1*, Heebak Choi 1*, Ping Jin 1*, Yunfei Wu 1, Jinmi Yoon 1, Yang-Seok Lee 2, Taiyong Quan 3, Gynheung An Yield and quality traits are tightly related to flowering time. Most high-yield rice cultivars flower late, whereas high-quality cultivars flower early. Shortening flowering time without quantity reduction or delaying flowering time with maintaining seed quality are in demand. In this study we elucidated genetic and environmental factors that control flowering time. First, we analyzed the effects of SD treatment on flowering time. Second, we examined the effect of exogenous sucrose on controlling flowering time. Third, we performed a screening of T-DNA tagging lines to find a promising genetic factors. Screening T-DNA tagging population resulted in identification of two early flowering mutant lines where expression levels of Roc4 gene were significantly enhanced. The early flowering phenotypes were observed in LD conditions but not SD conditions, similar to ghd7. Further experimental results lead our conclusion to the identification of Roc4 as flowering time inducer contributed to control flowering time in rice and it showed potentials for improving quantity traits through modulating Ghd7 expression. *Corresponding Author: genean@khu.ac.kr (G. An) OA-04 Ravi Gupta 1, Cheol Woo Min 1, Ramesha H. Jayaramaiah 1, Sun Tae Kim 1* 1 Phytohormones are the chemical messengers that regulate plethora of biological processes throughout the life cycle of a plant. Although several components related to the biosynthesis and signaling of these phytohormones have been identified, a deep understanding of downstream targets is missing, especially at the protein and metabolite levels. Therefore, here we used an integrated physiological, proteomics and metabolomics approach to investigate the ethylene, ABA and combined ABA+ethylene signaling in soybean leaves. A protamine sulfate precipitation (PSP) method was employed to enrich the low-abundance proteins followed by their identification and quantification using label-free quantitative proteomics. This approach allowed the identification of 5171 unique proteins and 1182 differentially modulated in one or more treatments. Moreover, phosphoproteome analysis led to the identification of 716 class 1 phosphorylation sites (localization probability 0.75, score difference 5) belonging to 532 unique phosphoproteins. In particular, increased phosphorylation of MPK3/6 was observed after ethylene treatment while ABA resulted in dephosphorylation of these MPKs. Functional annotation of the identified proteins showed an increased abundance of proteins related to the flavonoid and isoflavonoids biosynthesis in response to ethylene treatment and a shift in the fatty acid metabolism upon ABA treatment. HPLC analysis showed an accumulation of isoflavones (Genistin, Daidzein, and Genistein) upon ethylene treatment, validating the proteomics results. Further, metabolome analysis using LC-MS/MS confirmed the accumulation of flavonoids and isoflavonoids in response to ethylene treatment and accumulation of lipids in response to ABA treatment. Taken together, our results showed potential cross-talks between ethylene and MPK-signaling and ABA and lipid signaling pathways. *Corresponding Author: stkim71@pusan.ac.kr 22

65 OA-05 Sang-Kyu Lee *, June-Seob Eom, Seok-Hyun Choi, Dae-Woo Lee and Jong-Seong Jeon To elucidate the starch synthetic pathway and the role of this reserve in rice pollen, we characterized mutations in the plastidic phosphoglucomutase, OspPGM, and the plastidic large subunit of ADP-glucose (ADP-Glc) pyrophosphorylase, OsAGPL4. Both genes were upregulated in maturing pollen, a stage when starch begins to accumulate. Progeny analysis of self-pollinated heterozygous lines carrying the OspPGM mutant alleles, osppgm-1 and osppgm-2, or the OsAGPL4 mutant allele, osagpl4-1, as well as reciprocal crosses between wild type (WT) and heterozygotes revealed that loss of OspPGM or OsAGPL4 caused male sterility, with the former condition rescued by introduction of the WT OspPGM gene. While iodine staining and transmission electron microscopy analyses of pollen grains from homozygous osppgm-1 lines produced by anther culture confirmed the starch null phenotype, pollen from homozygous osagpl4 mutant lines, osagpl4-2 and osagpl4-3, generated by CRISPR/Cas system, accumulated small amounts of starch, which were sufficient to produce viable seed. Such osagpl4 mutant pollen, however, was unable to successfully compete against WT pollen, validating the important role of this reserve in fertilization. Our results demonstrate that starch is polymerized mainly from ADP-Glc synthesized from plastidic hexose phosphates in rice pollen, and that starch is an essential requirement for successful fertilization in rice. *Corresponding Author: jjeon@khu.ac.kr OA-06 Keunpyo Lee *1, Junkyoung Choe 2, Younghoon Park 3, Jin-Hee Lim 4, Byung-Chun In 4, Sung-Chur Sim Advance of next generation sequencing (NGS) techniques and bioinformatics has led to dramatic increase of genome data in crop species. The linkage between genotype and phenotype is essential to develop molecular tools, which can accelerate to improve agronomically important traits in breeding programs. In the present study, we developed a prototype of the 'Phenome and Genome Database for Breeding (PGDB)' using the tomato data. The PGDB provides not only high-quality phenotypic data, but also SNP markers for traits of interest. For the phenotypic data, we selected traits based on Plant Ontology System, UPOV test guideline, and comments from breeders and used a collection of 314 accessions representing contemporary varieties, vintage varieties, and wild species. The SNP genotypes of tomato varieties were produced using the SolCAP array consisting of 7,720 genome-wide SNPs and the publicly available resequencing data. With these phenotypic and genotypic data, this web-based database provides multiple options to find molecular markers associated with traits of interest. Thus, the PGDB is a useful breeder s toolbox to facilitate cultivar improvement in tomato breeding programs and could be further extended for other crops. *Corresponding Author: kplee@korea.kr 23

66 OB-01 Je Hyeong Jung 1*, Ja Kyong Ko 2, Sun-Mi Lee Lignocellulosic biomass has been considered one of the most promising alternatives to fossil resources for the production of fuels and fine chemicals. However, bio-refinery from biomass into bio-based products remains suboptimal primary due to the recalcitrant nature of biomass. Plant cell walls of lignocellulosic biomass are highly heterogeneous, composed of various polysaccharides and aromatic compounds in the form of (hemi)cellulose and lignin. Simple sugars recovered from (hemi)cellulose are utilized as the main substrate for bioconversion, while lignin prevents the efficient hydrolysis of (hemi)cellulose into fermentable sugars by forming a physical barrier and non-productively adsorbing hydrolyzing enzymes. In this respect, reduced lignin content and/or altered lignin structure have been desirable traits for the biomass feedstock, and modifying lignin biosynthetic pathway has been a straightforward strategy to improve the bioconversion efficiency. Genome editing technologies enables rapid introduction of desired traits by precise sequence modification and controlled incorporation of fine-tuned gene units. Here, we reports targeted mutagenesis of one of the lignin biosynthetic genes, caffeic acid O-methyltransferase (COMT) in sugarcane and barley through TALEN and CRISPR/Cas9, respectively. Targeted mutation of COMT resulting in reduced lignin content led to a substantial increase in fermentable sugars and bio-ethanol yields. In addition, a strong synergism in elevating ethanol productivity was created by the combination of utilizing lignin reduced biomass and deploying engineered yeast strain co-fermenting both xylose and glucose. *Corresponding Author: jhjung@kist.re.kr OB-02 Cheol seong Jang As sessile organisms, plants are constantly exposed to adverse environmental stresses, such as high salinity, water deficit, and temperature fluctuation. Therefore, plants have evolved mechanisms that allow them to adapt and survive in various adverse environmental conditions. The proteins harboring RING finger motif(s) have been shown to mediate protein protein interactions that are relevant to a variety of cellular processes including abiotic stress adaptation. To elucidate the evolutionary dynamics of the rice RING finger protein family, a total of 488 potential RING finger protein genes were retrieved via in silico analysis from the rice genome and then divided into groups on the basis of the presence of zinc-coordinating Cys and/or His residues in their RING domains. In this study, molecular dissection of four was performed by examining their E3 ubiquitin ligase activity and subcellular localization, and determining their substrate proteins. First, OsHCI1 was specifically induced by heat and cold stress treatments, and its subcellular localization was shown to be mainly associated with the Golgi apparatus and changed rapidly and extensively moving along the cytoskeleton. Thus, OsHCI1 may have accumulated in the nucleus under high temperatures. In addition, heterogeneous overexpression of OsHCI1 in Arabidopsis highly increased the survival rate through acquired thermotolerance. Secondly, OsCTR1 was highly expressed under dehydration and defense related phytohormones, and its encoded protein was localized in both the chloroplasts and the cytosol. Two chloroplast-localized proteins (OsCP12 and OsRP1) interacted with OsCTR1 in the cytosol, and ubiquitination by OsCTR1 led to protein degradation via the Ub 26S proteasome. The Oryza sativa RING finger protein with microtubule-targeting domain 1 (OsRMT1) is a functional RING E3 ligase likely involved in a salt mechanism. Functional characterization revealed that OsRMT1 undergoes homodimer formation and subsequently autoubiquitination-mediated protein degradation under normal conditions. The other protein OsSAD1 was highly induced in response to high salinity, dehydration treatment, and ABA stresses. For the results of Yeast-Two hybridization, in vivo protein degradation assay supports that OsSAD1 interacts with 3 substrates namely OsSNAC2, OsGRAS44, and OsPIRIN1, and mediates proteolysis of these 3 substrates via the 26S proteasome pathway. In conclusion, these comprehensive studies provide insight into the biological function of OsRFPs, which may be useful in understanding how rice plants adapt to unfavorable environmental stresses. Corresponding Author: Tel , csjang@kangwon.ac.kr 24

67 OB-03 Inchan Choi, Hyeonso Ji, JeongHo Baek, Song Lim Kim, Taek-Ryoun Kwon, Kyung-Hwan Kim * Considerable progress has been made in plant genotyping with the establishment of next generation sequencing methods. However phenotyping is a limiting factor in crop development, which makes it difficult to develop accurate and large scale genetics and breeding. Recently, plant phenomics has emerged as a new research area through high-throughput analysis of plant traits such as plant structure, water contents, physiological status etc. using image analysis. RDA has been setting a high-throughput crop phenotyping (HTCP) facility with RGB, IR, and NIR image analysis instruments. In addition, robotic XYZ phenotyping systems with RGB, 3D-Laser, and fluorescence cameras are being installed. The crop plant phenotyping facility consists of an environmental control greenhouse, image acquisition devices, conveyor, watering system, DB and data management programs. As the first trial to utilize the HTCP system in real study, we are analyzing key characteristics of rice plants such as height, leaf area, and leaf color etc. using RGB images. Besides, the color of the soybean seeds and the flower shape of the Phalaenopsis were analyzed with the image technology. This facility shall continue to be utilized for analysis of various target traits of rice plants such as abiotic stress tolerances and yield components in the future. *Corresponding Author: biopiakim@korea.kr OB-04 Jong Tae Song We recently isolated the DDMP-deficient saponin mutants in soybean [Glycine max (L.) Merr.]. In soybean, two groups of saponins, group A and DDMP saponins were reported till now. Group A saponins are undesirable component of food products due to its bitterness and astringency. In contrast, DDMP saponins and their derivatives are not possessing bitterness and astringency but has more beneficial to human health than group A saponins when consumed as regular diet. However, DDMP saponin biosythesis and associated genes did not come to limelight yet. Therefore, we utilized two EMS mutant lines (PE2248 and PE2371) with DDMP-deficient saponins to identify and characterize the gene which is encoding a protein responsible for biosynthesis of DDMP saponins, and then finally to reveal a role of saponins in soybean plants. The breeding cross has been made with these two mutants along with two cultivars, Pungsannamul and Uram to study the segregation and genetic linkage analysis, respectively. The segregation analysis showed that the mutant phenotype is controlled by single recessive gene. The position of locus (Sg-9) involved in the biosynthesis of DDMP deficient saponins was mapped using bulk segregation analysis and fine mapped on chromosome 16 (130 kb) between two SNP markers. Sequence analysis of Sg-9 gene revealed a single nucleotide polymorphism in PE2248 (G626A) and PE2371 (C137T) mutant lines. In addition, we verified the mutant alleles by complementation analysis between two mutants. Corresponding Author: jtsong68@knu.ac.kr 25

68 OB-05 Jelli Venkatesh 1, Li Liu 1, Min-Young Kang 1, Jin-Kyung Kwon 1, Yeong Deuk Jo 1, Jin-Ho Kang 1, Sota Koeda 2, Munetaka Hosokawa 2, Sandra Goritschnig 3, Byoung-Cheorl Kang 1* Capsicum chinense sy-2 is a low temperature-sensitive natural pepper mutant. The low temperature-sensitivity of the sy-2 is controlled by a single recessive gene, sy-2 located on pepper chromosome 1. In the present study, the sy-2 locus was fine mapped using F 2 mapping populations developed from across between C. chinense sy-2 and No Based on genetic linkage analysis and genomic information of pepper, the sy-2 gene was delimited to an interval of kb region flanked by SNP 5-5 and SNP 3-8 markers. Two putative F-box genes, ORF10 and ORF20 were identified as candidates for low temperature sensitivity from the sy-2 target region based on the relative gene expression analysis. The sy-2 plants under low temperature exposure (20 C) had a lower expression level of these F-box genes than wild-type plants, even under 28 C growth temperature. Virus induced gene silencing (VIGS) of ORF10/ORF20 genes in wild type pepper, No.3341 resulted in plants with abnormal leaves similar to as that of sy-2 mutant pepper under low temperature. Silencing of ORF10/ORF20 homologs in Nicotiana benthamiana has also resulted in the development of similar mutant phenotype. Protein-protein interaction studies suggest that ORF10 and ORF20 are part of Skp, Cullin, F-box (SCF) complex proteins, which plays important roles in proteasome-mediated degradation of proteins. Taken together, these results suggest that the sy-2 candidate F-box proteins, as a component SCF complex protein could play an important role in plant growth and development under low temperature conditions. Further functional characterization of these F-box genes would offer new insights into the molecular and physiological mechanisms of low temperature sensitivity in pepper. *Corresponding Author: bk54@snu.ac.kr OC-01 Sang-Tae Kim, Hyeran Kim, Je Wook Woo, Hee-Jung Sim, Jae-Young Yun, Hyo-min Ahn, Jahee Ryu, Min Kyung Choi, youjin Shin, Beum-Chang Kang, Suji Bae, Sunyoung Moon, Jin-Soo Kim, Sang-Gyu Kim * Genome editing tools have been developed to manipulate genomic DNA in animals and plants. In particular, the recent genome editing tool, CRISPR/Cas9, allows us to induce targeted mutagenesis with high efficiency and low cost. As the PCR (polymerase chain reaction) has revolutionized molecular biology, the CRISPR system will become a key and basic technique to answer many biological questions. In addition, the CRISPR system will be widely used in applied science, such as gene therapy, drug development, and livestock and crop breeding. In this talk, I will talk about how to do gene editing with CRISPR/Cas9 system for plant genome editing. Especially, I will suggest a basic guide line for the beginner: how to design a guide RNA, how to check a mutation frequency, and how to deliver Cas9-guide RNA into a plant cell. Finally, I will talk about the new CRISPR system, base-editor for targeted nucleotide substitutions. *Corresponding Author: sgkim@ibs.re.kr 26

69 OC-02 Hyeran Kim Genome editing has been recently democratized by the development of RNA-guided endonucleases (RGENs) repurposed from the type II CRISPR/Cas9 prokaryotic adaptive immune system. CRISPR/Cas9 and CRISPR/Cpf1 system have been rapidly implemented for plant genome editing and crop breeding. Crop improvement is essential to attaining world food security and enhancing nutrition for human beings. Both conventional breeding and modern molecular breeding have contributed to increased crop production and quality. However, the time and resources for breeding practices have been limited. It takes a long time to bring a novel improved crop to the market, and the genetic sources from wild species cannot be always available for crops of our interests. Genome editing-mediated molecular breeding can overcome those limitations of time and resource by facilitating the specific editing of plant genomes. We successfully set up versatile CRISPR/Cas9 and CRISPR/Cpf1 systems for plant research and crop breeding. In this seminar, RGENs (Cas9 and Cpf1)-mediated soybean genome editing and our recent studies will be shared and discussed. Corresponding Author: ranny@ibs.re.kr / ranny96@gmail.com OC-03 조성환 *, 이봉우, 김지은, 최준경, 김웅범, 오재은, 이정희 신품종육성은다양한분야의지식과경험이한데어우러져만들어지는총체적인산물로생각할수있다. 최근유전체정보를포함하여다양한오믹스데이터와생물정보학기술이크게발전하면서혁신적인육종시대를앞당기고있다. 그럼에도불구하고데이터가지니는다양성, 복잡도, 대용량의크기등으로인해독립된각자의팀에의해실용적활용에한계가발생하고있다. 심지어는절망을경험하기도한다. 따라서데이터를생산하거나확보하는차원에서그치는것이아니라, 어떻게데이터로부터얻은정보를효율적으로활용하여우리가원하는신품종을개량해갈수있을지고민하게되었다. 그중하나의시도는오믹스정보및분석기술, 아이디어등을함께공유하고, 공동의문제를함께풀어갈수있는오픈플랫폼을구축하는것이다. TGsol 은가지과작물 ( 토마토, 고추, 감자 ) 의유전체육종을구현하도록돕는것을목적으로농생명게놈활용연구사업단의지원을받아 2012 년부터구축하여공개해왔다. 지난수년간표준유전체와 250 여계통의토마토유전자원 resequencing 결과및고추 RIL resequencing 결과를이용하여수백만의 genome-wide SNP 와다양한정보를확보하였다. 또한모든정보는사용자가검색하여이용할수있도록제공하고있으며, 육종활동에필요하다고판단되는다양한툴을개발해왔다. 그러나수많은데이터확보에도불구하고데이터베이스의활용이라는측면에서한계를느끼게되었다. 만들어놓은정보를검색하여제공하는수준으로는해외선진연구팀혹은산업체와경쟁이어렵다는것을인식하였다. 이제는많은전문가의참여를통해상호간의이해의폭을넓혀실질적인활용사례를만들고, 문제점을해결해나갈수있는통로를확보하는것이절실하게필요하다는것을배우게되었다. 이를위해서는사용이편리한플랫폼을만드는것뿐만아니라서로의정보를공유하는것이서로에게도움이된다는것을이해하고실천하는것이필요하다. 이를위해서 TGsol 웹사이트내에의견을공유할수있는공간을만들어가면서다양한의견교류가정보기반의육종을앞당기기를기대해본다. (TGsol web site: * 주저자 : Tel , shjo@seeders.co.kr 27

70 OC-04 유의수 *, 최범순, 김남훈, 이현오, 박미소, 이승욱 차세대유전체염기서열분석및생물정보학의비약적발전은많은유전체정보를신속하고효율적이며저비용으로생산, 해석할수있는방법을제공하였고, 이을바탕으로표준유전체해독및유전체또는작물고유의특성을통합적인해석을통해이해하기위한많은사업들이진행되고있다. 또한 resequencing/gbs 와같이응용된 NGS 기술을이용하여주요작물의유전변이를대량확보하고이를분자육종에이용하고자하는유전체기반분자육종이대두되고있다. 최근국제미작연구소 (IRRI) 와중국 BGI 에서진행한 rice 3k project 는 95 개국에서수집된재래 / 재배벼 3,000 점을 4-60x 정도로 requencing 을하여대량의유전변이을수집하였고이를표현형과의연관분석을통해다양하고복잡한양적형질의동정에활용할계획이다. 이런추세는농업전분야로확대되어대량의유전체데이터의생산및분석이이루어지고있어효율적생물정보분석시스템의개발이향후데이터를생산, 해석하고이를분자육종에효율적으로적용하는데반드시필요한분야로여겨진다. 분자육종의효율성을가속화하기위해서본유전체기반분자육종시스템구축과제는다양한유전체분석도구들의개발로효율적인유전체정보의활용과분석이될수있도록하는시스템을구축하고자한다. 주요내용으로는 1) 다양한유전체정보 ( 유전체, 전사체, SNP 정보, 분자마커정보, 표현형정보등 ) 를수집하여통합유전체데이터베이스를운영하고, 2) NGS 기반의 SNP genotyping 에필요한효율적파이프라인을구축하고, 3) 유전체정보와변이정보를연동하여 visualization 할수있는브라우저와분자마커개발에필요한도구의개발이다. 본발표를통해서비교유전체도구, 분자마커개발파이프라인및시각화툴등과제수행에서구축된도구들을소개하며앞으로의방향을소개하려한다. 이런노력을통해서다양한유전체정보를분자마커개발, QTL 탐지, 후보유전자동정등분자육종에효율적으로활용할수있게하며, 분자육종의선진화에기여하고자한다. * 주저자 : Tel , yeisooyu@phyzen.com OC-05 Lincoln Ong 본세션에서는 LGC 그룹이보유하고있는첨단대규모육종지원시스템을소개하고대규모분자육종에효율적으로활용할수있는다양한지원시스템을소개하고자합니다. LGC 그룹은영국런던에본사를둔글로벌라이프사이언스회사로서 1842 년회사창립을시작으로올해 175 년의장대한역사를지닌그룹입니다. LGC 그룹은실험실서비스, 측정과계측관련표준및기준물질, 유전체및분석시험분야에있어글로벌리더로서전세계 22 개국가에제품공급및서비스를하고있으며, 글로벌시장에발맞춰전세계거점지역내 9 곳의제조라인과 3 곳의특화된서비스연구소가있습니다. LGC 그룹내 Genomics 비지니스유닛은리서치, 진단및적용분야에있어토탈유전체솔루션을제공하는글로벌리더입니다. LGC 제품군은 end-point PCR 및 qpcr 분야에있어최상의시약, 특화된기기와고객니즈에맞춘서비스를제공하는토탈포트폴리오입니다. 시약으로는전세계적으로사용되는 KASP, BHQ 프로브등이있으며장비로는 Array Tape 을기반으로한전자동최신시스템인 Nexar, DNA extraction 을위한최적의기기인 Oktopure 및 Genotyping 의장비군인 SNPline 과더불어 PCR 플랫폼과 liquid handling 이통합운영되는 IntelliQube 등이있습니다. 이와더불어 Genotyping, DNA extraction, Array, GBS(Genotyping by Sequencing), Sanger sequencing 과 NGS 등, LGC 의혁신적인기술과서비스를통해고객의니즈에맞는프로젝트서비스와랩서비스가제공되고있습니다. 보다자세한 LGC 그룹에대한내용은웹사이트 ( 또는국내 LGC genomics 담당자인정미영이사 (michelle.jeong@lgcgroup.com) 에게연락주시기바랍니다. 감사합니다. 28

71 OC-06 정윤재, MS.Eng., MBA This presentation describes how to perform genotyping of low-concentration DNA with the Juno Genotyping IFC (integrated fluidic circuit) on the Juno system. This is possible through advanced microfluidics technology that integrates preamplification and genotyping reactions of up to 96 samples and 96 genotyping assays in a single workflow on an IFC. The IFC produces 9,216 genotypes in less than three hours using a simple workflow with minimal hands-on time. Samples are loaded into individual inlets of the Juno Genotyping IFC, then distributed across multiple reaction chambers in nanoliter-volume aliquots. With high-quality samples, detecting the specific targets requires thermal cycling for preamplification and PCR for genotyping on the instrument. After genotyping is performed on the Juno system, the IFC is scanned on the EP1 system or the Biomark HD system to collect genotyping data for later analysis. Corresponding Author: Ken.jung@fluidigm.com OD-01 최근진, 오동진, 양미희, 김태영, 김진욱 종자산업은농업에있어서가장기본이되는자산이며생명산업및 ICT 등과융합하여미래의고부가가치산업으로서가능성이높은산업이다. 따라서다국적기업들이종자산업에참여하여시장을점유해가고있는형편이며특히국제적으로는듀폰의다우인수, 캠차이나의신젠타인수, 바이엘의몬산토인수와국내적으로는 LG 화학의동부팜한농, 농협의농우인수등의변화가있었다. 우리는이러한대내외환경변화에적극대처하고국내종자기업이세계시장에서경쟁력을갖출수있도록새로운육성정책을발굴하고지원할필요성이있다고할것이다. 이를위해그동안정부는민간육종연구단지의조성, 방사선육종센타의설립그리고 GSP 사업을추진해오고있다. 그러나세계종자시장의급격한증가및변화에도불구하고국내종자시장의규모는거의증가가없는상태이다. 이는파종기술의기계화및정밀화, 종자시장의포화, 경지면적감소등의원인이있는것으로볼수있다. 따라서정부는종자산업의발전을위해세계종자시장의점유율을확대하고글로벌및우수종자기업을육성하기위해종자산업의시장규모를확대하고종자산업기초향상및기반구축, 시장주도형신품종개발, 우수종자공급, 유통질서확립및종자기업의경쟁력향상을추진할계획이다. 또한종자산업의세계화를위해서는수출시장의다변화및새로운수출시장의개척에목표를두고수출전용품종의육성, 품질관리강화, 신수요의창출, 국제협력의확대를추진해나갈계획이다. 교신저자 : Tel , kjchoi1001@korea.kr 29

72 OD-02 강항구 Sweet Corn 동남아지역개발에있어서필수적인고온및우기잘자랄수있는특성을가진품종개발 (Tropical Type) 이매우중요하다 년인도및인도네시아에서시험포사업을여러차례실시하여매우긍정적인결과를얻었다. 인도에서는 1 품종이상업화되었으며 1 조합이매우유망한조합으로선발되었다. 인도네시아에서는 3 조합이선발되어 2017 년도에확대시험할예정이다. 인도및인도네시아선발조합들이같은조합들은없었다. 인도네시아기후조건이인도보다열악하므로인도네시아조합들은초세및내병성부분에서인도요구특성보다훨씬강한특성을지닌방향으로육성을해야할것이다. 동남아지역 Sweet Corn 시장은아직까지큰시장이형성된것은아니나지속적으로시장규모가증가추세에있으므로향후시장성은매우크다고예측되며, 해외법인국가 ( 인도, 인도네시아및중국남부 ) 를중심으로적극적으로우선개발계획을가지고있으며그외국가들도점차적으로개발을확대할계획이다. OD-03 박형준 종자시장은품종의기술적인경쟁력이가장중요한 Product Driven Market 이다. 특히품종들의유전적특성의차별화를만들어내기힘든상황에서는 Management Competition 또한품종개발성공의중요한한축이다. 기술적인차별화의중요한부분들을활발히육성하는것이전체종자사업의중요한한 chain 이다. 즉종자산업에서는육성이외의여러다른축들이존재하므로, 품종육성을통한유전적품종의차별화뿐만아니라 supply chain 의또다른기능들을통해서차별화하는것도종자산업의중요한부분이다. 인도고추종자시장은현재수확량만가지고차별화되는 1 단계를넘어인도고유의품종적요구도와내병성을겸비한 3 단계로발전한시장으로급속히이동하고있으므로, 전체적인 supply chain 에서의차별화를만들어내지않으면시장에서성공하기힘들다. 교신저자 : paul0525@naver.com 30

73 OD-04 유재흥 1*, 이종훈 1, 안경구 1 1 세계양배추재배면적은약 200 만 ha (FAO 2010 년자료 ) 로채소용배추과재배면적으로는가장넓은재배면적의작물로전세계으로널리재배및섭취되고있으며, 전세계종자소요량은 500 여톤, 시장규모는판매가격기준으로 1 억 7 천만불 ( 약 1900 억원 ) 이상으로추정된다. 전체양배추재배면적중 3 분의 2 에해당하는지역이아시아이며중국인도의재배면적이 100 만 ha 이상으로추정되고, 유럽은약 42 만 ha 로약 20% 내외로추정된다. 양배추주요생산및소비지역인유럽과아시아지역의양대시장규모는각각약 7 천 3 백만불, 6 천 9 백만불로전체의 80% 이상을차지하는것으로추정된다. 재배면적이제일큰아시아시장은여러국내회사들의노력으로일본및해외거대기업들과각축을벌일수있는수준으로발전을해왔으나고부가가치시장으로금액적인시장규모는가장큰유럽지역은네덜란드및일본회사들에비해국내회사들의진출이미미한상황이다. 글로벌양배추시장에서해외기업들과경쟁할수있도록시들음병, 검은썩음병, 뿌리혹병등주요내병성품종및저장성을높이는내열구성품종육성연구를통해유럽시장으로의진출과아시아및세계여러시장에도수출을증대시키고있다. 또한종자및농업관련무역회의참석을통한품종홍보활동강화및국가별핵심거래처를확보, 해외현지지역잡지를통한지속적인홍보와자체브랜드판매비율을확대함으로서브랜드력을강화하는활동등수출시장확대를위한마케팅전략강화활동도병행하고있다. * 주저자 : Tel , andy.yoo@joeunseeds.com OD-05 김명권 토마토는세계적으로과채류 (fruit vegetable) 중에서가장재배면적이많으며글로벌채소종자시장에서중요한위치를차지하고있다. 각나라마다재배환경, 소비형태에따라다양한토마토품종들이재배되고있다. 이에품종육종도지역별, 재배형태별, 이용방법에맞추어품종을육성하고있으며, 크게 protected cultivation and open field cultivation, fresh usage and processing usage 로나눌수있다. 육종형질적인면에서보면생육특성, 과품질, 내병성요소로, 생육특성은유한형무한형, 저온신장성고온신장성, 내건성내습성, 초세, 엽형, 절간, 화방발달등이고, 과품질은착과비대균일성, 색상의선명도, 어깨색, 경도, 열과저항성, 저장성, 당도, 식미, 성분함량등이며, 내병성은토마토황화잎말림바이러스 (TYLCV), 토마토모자이크바이러스 (ToMV), 토마토반점위조바이러스 (TSWV), 시들음병 (Fusarium), 반신위조병 (Verticillium), 근부위조병 (Fusarium radicis), 역병 (Phytophthora), 겹둥근무늬병 (Alternaria), 잎곰팡이병 (Cladosporium), 선충 (Nematode), 갈색근부병 (Pyrenochaeta), 흰가루병 (Powdery mildew), 풋마름병 (Bacterial wilt), 점무늬병 (Stemphylium), 세균성반점병 (Xanthomonas) 등이있다. 여러특성과형질들을 Target 시장에맞추어육종하여야하며, 현지적응성시험으로정확한평가와조직적인홍보마케팅활동, 대농민고객컨설팅이병형되어야만성공적인품종을개발할수있다. 교신저자 : Tel , mkkim207@naver.com 31

74 OD-06 조영일 최근종자산업은바이오에너지, 식품, 의약, 신소재등미래성장유망산업의원천이되는기반산업으로부각되고있으며, 종자관련기술이첨단 BT, IT 기술과융합하면서새로운고부가가치산업으로급성장하고있다. 이러한세계적흐름에부응하여정부는종자산업을미래신성장동력산업으로육성하기위한종자산업육성정책의일환으로육종 R&D 인프라구축및지원사업인 종자산업진흥센터 ( 민간육종연구단지 ) 를 2016 년 10 월전라북도김제시백산면에조성하였다. 종자산업진흥센터는민간종자기업의우수신품종개발과수출을확대할수있도록지원하는서비스전문기관으로민간육종연구단지내위치하여분자표지및기능성분석서비스등첨단육종기술지원서비스제공하고, 국제종자박람회개최등종자산업육성지원과산업전문인력양성교육및안정적공급, 민간육종연구단지운영 관리등의업무를수행한다. 종자산업진흥센터는민간종자기업의육종 R&D 를글로벌수준의차세대분자육종체제로전환하고, 글로벌시장경쟁력향상을위해몬산토, 신젠타등다국적종자기업이사용하고있는첨단분석장비 ( 주요장비 7 종 ) 을도입하여분자표지분석및기능성성분분석등맞춤형육종기술지원서비스를제공하고있다. 종자산업진흥센터의분자표지분석서비스는대용량자동화 DNA 추출장치, 대용량유전자분석장치등첨단분석시스템 3 종을국내최초로도입하였고, 고추, 배추, 벼등 9 개작물을대상으로확보된병저항성및특성검정, 종자순도검정, 품종판별, 여교배선발 (MAB : Marker-assisted backcross) 용분자표지등약 2,770 여개를이용하여종자기업이우수계통의조기선발과품종의특성을신속 저렴하게검정할수있도록분석서비스를제공하고있다. 또한고기능성계통선발과고품질의품종개발지원을위해국내종자기업을대상으로사전수요조사를통해선정된서비스요구도가높았던카로티노이드, 갭사이시노이드등 7 가지기능성성분에대해서도분석서비스를제공하고있다. 향후종자산업진흥센터는수요에따라식물종자분야뿐만아니라축산, 수산분야로도단계적으로서비스항목및영역을확대해나갈예정이다. 교신저자 : breedy01@efact.or.kr 32

75 한국육종학회구두발표

76

77 OE-1 Nomar Espinosa Waminal 1,2, Remnyl Joyce Pellerin 1, Sung-Min Youn 1, Tae-Jin Yang 2* and Hyun Hee Kim 1* 1 2 Fluorescence in situ hybridization (FISH) is a highly efficient molecular cytogenetic tool to visualize distribution of DNA elements in a genome. Conventional FISH procedure involves preparation of fluorescence-labeled probes that is time-consuming and requires technical experience. To improve this process, we designed oligodeoxynucleotide (ODN) probes for three commonly used targets, 5S, 45S rdna, and telomere repeats. We designed twelve, four, and one ODN probes for 45S rdna and 5S rdna, and telomere tandem arrays, respectively. The rdna probes has universal utility for plants including gymnosperms and angiosperms, animals, and fungi and telomere probe can be utilized for most of plants harboring Arabidopsis-type, (TTTAGGG)n, telomere array. Use of different fluorochromes conjugated to the 5 end of each ODN probes permitted the simultaneous visualization of the genomic loci of the three target sequences which are commonly the most distinct feature of a chromosome complement. Utilization of pre-labelled ODN probes reduced efforts and time for FISH analysis. It greatly reduced the FISH hybridization time from ~16h at 37 for conventional FISH to as short as 5 min at room temperature. The ODN probes can be ideal for routine FISH analysis even for species without preliminary chromosomal data. The method is efficient, reliable, and rapid in simultaneously detecting several target repeats, and is particularly useful in rapid chromosomal analysis of crop collections in breeding studies. Acknowledgement: This work was carried out with the support of "Cooperative Research Program for Agriculture Science & Technology Development (PJ )" Rual Development Administration, Republic of Korea. *Corresponding Author: Tae-Jin Yang: Tel , tjyang@snu.ac.kr Hyun Hee Kim: Tel , kimhh@syu.ac.kr OE-2 정규미, 금보라, 이준대 * 파프리카의경제적인일대잡종종자를생산하기위해노동력과비용을절감할수있는유전자적웅성불임 (genic male sterility, GMS) 시스템을사용하고있다. 기개발된 PmsM1-CAPS 분자표지는유전자적웅성불임유전자인 ms 1 으로부터약 2-3 cm 떨어진곳에위치해있기때문에제한적으로사용되었다. 따라서본연구에서는파프리카 1,118 개체 F 2 분리집단을사용하여 ms 1 유전자좌고밀도유전자지도를작성하였으며, NGS 분석을통해얻은 SNP 정보를이용해 ms 1 유전자좌에아주가깝게연관된 12 개의 HRM 분자표지를개발하였다. 또한이분자표지를이용하여재조합이일어나지않은 kb 구간을설정할수있었고, 그곳의염기서열을 FGENESH 웹프로그램을이용하여분석한결과, 11 개의 open reading frames(orf) 이존재함을확인하였다. 그중가장강력한후보유전자는 CA05g06780 으로 Arabidopsis MALE STERILITY 1 (MS1) 유전자와아주높은상동성 (homology) 을보이고있었는데, Arabidopsis 에서 MS1 유전자는 plant homeodomain(phd)-type 전사인자로서화분 (pollen) 과융단조직 (tapetum) 의발달을조절하는기능을가지고있다고보고되었다. 따라서가임 (male-fertile, MF) 과불임 (male-sterile, MS) 파프리카의 CA05g06780 유전자를염기서열분석한결과, 불임유전자에서 1 bp 의결실 (deletion) 이발견되었다. 이결실은 2 번째엑손 (exon) 내에서발생하였는데, 이는 3 번째엑손에서 premature stop codon 을야기시켜가임단백질 (664 개의아미노산 ) 보다훨씬짧은단백질 (197 개의아미노산 ) 을만들도록영향을미치는것이었다. 이러한정보를바탕으로 1 bp InDel 을구분할수있는 luna probe HRM 분자표지를개발하여다양한파프리카재료에테스트한결과마커형과표현형이완벽히일치함을확인하였다. * 교신저자 : Tel , ajfall@jbnu.ac.kr 35

78 OE-3 Dong-Seon Kim 1*, Juyeon Jung 2, Yeon-Ok Choi 2, Sunggil Kim 1* 1 2 Eleven SRK-homologous genes were identified from two draft genome sequences of radish (Raphanus sativus L.). The phylogenetic tree of the SRK and its homologs showed that all homologs were more closely related to the class II SRK genes compared with class I SRKs. Three homologs were shown to be tightly linked to the S locus. To develop efficient and reliable S locus haplotyping system, partial sequences of the tightly linked SP6 and SRK genes were isolated from 35 diverse breeding lines showing differential self-incompatibility responses. A total of 24 SP6 and 29 SRK alleles were isolated. Whereas the phylogenetic tree of the SP6 alleles showed species-specific clustering patterns, the phylogenetic tree of the SRK alleles revealed intergeneric pairing of some SRK alleles. Among 35 breeding lines, 14 have combinations of the unique SLL2 and SP6 alleles, but the others shared a common allele of either SLL2 or SP6 genes. Except for two breeding lines which contained the same SLL2, SRK, and SP6 alleles, all breeding lines were shown to harbor unique haplotypes consisting of the SLL2, SRK, and SP6 alleles. When 57 additional diverse breeding lines were analyzed by this new S locus haplotyping system, haplotypes of all breeding lines were clearly identified. In addition, four novel haplotypes containing the same SRK allele but different SP6 alleles were identified. *Corresponding Author: Tel , dronion@jnu.ac.kr OE-4 Ho Jun Joh 1, Junki Lee 1, Sae Hyun Lee 1, Hyun-Oh Lee 2, Sang-Choon Lee 1, Tae-Jin Yang 1,3* Peucedanum japonicum, a perennial herbal plant species belonging to the Apiaceae family, is distributed around coastal regions in Korea, Japan and Taiwan. In Korea, the leaves and roots are utilized as edible vegetables and oriental medicine named Sik-Bang-Poong, respectively. However, like most medicinal plants, very few genomic studies have been performed for this plant species. In this study, we have collected wild accessions from Jeju, Wan-do and Geumo-do and then characterized complete chloroplast (cp) genomes and 45S nuclear ribosomal DNA sequences by de novo assembly using next generation sequencing data of whole genome. Comparative analysis identified size and structural variations among cp genomes of P. japonicum accessions. In particular, two types of inverted repeats (IRs), short IR and long IR, were identified, which were correlated with sequence polymorphisms in other regions of cp genomes. Taken together, sequences and polymorphisms identified in this study will be valuable genetic resources that can be applied for molecular breeding of P. japonicum. Acknowledgement: This research was supported by Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ ), Rural Development Administration, Republic of Korea. *Corresponding Author: Tel , tjyang@snu.ac.kr 36

79 OE-5 Jin Sol Park 1, Hye Jeong Kim 1, Hyun Suk Cho 1, Jae Seong Kim 1, Wan Woo Yeom 1, Jae Yong Han 1, Dae-Jin Yun 2, Young Soo Chung 1* 1 2 YUCCA6 gene was introduced to produce drought tolerant transgenic soybean plants via Agrobacterium-mediated transformation using the improved half-seed method. The presence of the gene in transgenic plants were confirmed by PCR and Southern blot analysis, and the expression was investigated by RT-PCR. Transgenic line #2, #3 and #5 were tolerant to drought stress while non-transgenic plants were withered completely. Line #2, #3 and #5 were not affected remarkably by water deficit condition and lead to enhanced drought tolerance due to the prevention of cell membrane damage and maintenance of chlorophyll content. Moreover, the enhanced drought tolerance in transgenic lines resulted in reduced transpiration rate and low ROS content. *Corresponding Author: Tel , chungys@dau.ac.kr OE-6 Goh Choe *, Yogesh Gupta, Ana Cecilia Aliaga Fandino, Ji-Young Lee The Root system has two important functions for plants, providing mechanical support to the aerial parts of the plant by anchoring the plant body to the ground; and absorbing nutrients and water from the soil to distribute them throughout the plant. To better adapt to the environment, some plant species evolved to fortify these functions by increasing the root girth through secondary growth. The secondary growth has its importance in biomass accumulation as well. Many plants utilize roots as storage organs of carbohydrates produced during photosynthesis. The demands for plant biomass is increasing rapidly in the food market as well as energy industry. A continuous breeding effort aiming at improving biomass production is required. Radish, which specializes on the biomass allocation to roots with vigorous radial growth characteristics, can be a good candidate for the breeding purposes and for understanding secondary growth in plants. Although the molecular mechanisms underlying these events are still elusive, it is known that secondary growth originates at the vascular cambium which is located between the xylem and phloem. Vascular cambium divides periclinally to provide additional cell layers which will be differentiated into secondary xylem and phloem cells to the inside and outside respectively, contributing secondary growth capability. To identify important regulators facilitating root secondary growth, we collected laser-dissected tissue samples including cambium, cortex, and parenchyma from radish inbred lines showing contrasting secondary growth, and generated RNA-seq data. Novel transcripts were identified from de novo transcriptome assembly. Differentially expressed genes in cambium were isolated and analyzed. *Corresponding Author: gohchoe@snu.ac.kr 37

80 OE-7 Fenglin Deng 1, Naoki Yamaji 2, Jian Feng Ma 2, Enrico Martinoia 3, Youngsook Lee 1, Won-Yong Song 1* Rice is a staple food for billions of people in Asia, and its consumption is increasing in Europe and the USA, because it is regarded as a healthy, gluten-free alternative source of carbohydrates. However, the As content of rice poses a serious problem. As is a poison, and chronic exposure, even in low doses, can cause skin problems, bladder disease, and cancer. Particularly alarming are baby foods based on rice, which were considered to be nutritious and safe, but in fact contain relatively high amounts of As and can cause diseases and developmental problems in children. Reducing the As content in rice grains is therefore a very important issue for human health. To establish an effective technology for reducing As in rice grains, we devised a strategy to store As in the root and other vegetative organs of the plant, thereby inhibiting As from moving into the rice grain. This idea builds on results from our ongoing long-term research into the transport of toxic heavy metals and metalloids in plants. In the present work, we report the successful engineering of rice lines with reduced As in brown rice grains, using a tissue-specific promoter to drive the expression of genes encoding As transporters to sequester As into the vacuole. The engineered rice plants have lower levels of root-to-shoot and internode-to-grain As translocation, resulting in a 70% reduction in As accumulation in the brown rice grain. This work provides an innovative approach for reducing human exposure to As by making rice a safer source of calories and nutrition. *Corresponding Author: Tel , songwy@postech.ac.kr OE-8 Rupesh Tayade 1*, Tien Dung Nguyen, Lee Dasom 1, Sung Aeong Oh, Soon Ki Park * Adaptation of plants to global warming temperature is a challenging task. Current scenario is that heat stress and drought is becoming emerging abiotic stress causing serious threat to global food security. Enhancing the high temperature tolerance of crop species can facilitate the adaption of plant to ever changing environment and increase the yield. In Arabidopsis thaliana the C-repeat binding factor (CBF) transcription factor is involved in responses to low temperature and water deficit. In several plant species, over-expression of CBF genes leads to enhanced low temperature tolerance and growth inhibition. In the current study we generated transgenic Arabidopsis plants and observed the increased survival rate at heat stress condition (42ºC, 4h:30min) and, the delayed leaf senescence and flowering time compared to wild-type plant. In addition, it also observed the increased seed size. This result indicates that CBF1 gene may incorporate stress signals into developmental program for better adaptive growth under unfavorable conditions. *Corresponding Author: Tel , psk@knu.ac.kr 38

81 OE-9 Woojong Jang 1, Nam-Hoon Kim 2, Junki Lee 1, Nomar Espinosa Waminal 1, Sang-Choon Lee 1, Murukarthick Jayakodi 1, Hong-il Choi 3, Jee Young Park 1, Yeeun Jang 1 and Tae-Jin Yang 1* Korean ginseng (Panax ginseng C. A meyer) which has excellent pharmacological effects has been used as a valuable medicinal plant for a long time. In spite of its usefulness however, there has not been sufficient genetic information until now. Especially, its complex genome structure has not been elucidated. To understand its genome structure, we obtained ten random ginseng bacterial artificial chromosome (BAC) clone sequences through single-molecule real-time (SMRT) sequencing of a pooled DNA of the ten BAC clones. The ten assembled sequences corresponding to total 1,163,364 bp were obtained. Out of the ten BAC clones, nine were immaculately assembled in complete without any gap. The microcosm of ginseng genome were revealed that the 89.7% was composed of various repeat elements which have complex insertion patterns through careful sequence analysis. Especially, the large fraction of sequences were occupied by Ty3/Gypsy family long terminal repeat retrotransposons (LTR-RTs) as 46.9%. Six novel LTR-RTs were characterized the structure and their insertion time. Fluorescence in situ hybridization (FISH) analysis demonstrated that PgDel2 and PgDel5 elements had a subgenome-biased distribution. In remaining 10.3% of non-repeat regions of the 1,163 kb, only eleven protein-coding genes were identified. Collectively, our analysis reveals that ginseng genome has very complex genome structure with abundant repeats and rare gene frequency. The knowledge generated through this study provides various resources for understanding of ginseng genome and its breeding. Acknowledgement: This research was supported by Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ ), Rural Development Administration, Republic of Korea. *Corresponding Author: Tel , tjyang@snu.ac.kr OE-10 이현숙 1, 전윤아 1, 강주원 1,2, 안상낙 1* 1 2 벼재배에있어서노동력부족과임금상승등의이유로벼직파재배가점점늘어나는추세에있다. 이들직파재배에서유식물체토중출아율은잡초와의경쟁력을높이는중요한요인중하나이다. 벼유식물체의토중출아율은초엽과중배축신장성이관여한다고알려져있다. 본연구에서는재배벼 (Oryza sativa L.) Nipponbare 와 Kasalath 교배집단을이용하여중배축과초엽의양적형질유전자좌 (QTLs) 분석을실시하고토중출아율과의관계를조사하였다. Nipponbare 와 Kasalath 의 98 개여교배집단을이용하여토중 5cm 파종조건에서중배축신장관여 QTLs 은염색체 1, 3, 6 에서, 초엽신장관여 QTLs 은염색체 3, 5 에서탐지되었다. 이들 QTLs 에의한중배축또는초엽신장성신장조절이토중출아율에미치는영향을알아보기위하여염색체단편치환변이군 (chromosome segment substitution lines, CSSL) 을이용하여실험하였다. Nipponbare 와 Kasalath 유래 54 개 CSSLs 을토중 5cm 파종후 14 일동안토중출아율과중배축, 초엽신장성을조사하고상관분석을실시한결과중배축과출아율사이에는높은정의상관관계 (r> 0.6) 를보였으며초엽과는유의적인상관관계를보이지않았다. 이들 54 CSSLs 계통중에서중배축 QTL, qmel-1, qmel-3 을각각또는모두포함하는 3 계통, CSSL-6 (qmel-1), CSSL-15 (qmel-3), CSSL-5 (qmel-1 + qmel-3) 을선발하여토양깊이별출아율과중배축, 초엽의길이를조사하였다. 토양깊이는 3cm, 5cm, 7cm, 10cm 깊이별로종자를파종하고출아율을관찰한결과 7cm, 10cm 의깊이에서중배축신장성이가장좋은 CCSL-5 가 Nipponbare 나다른계통들에비하여가장빠른출아율을보였다. 본연구에서는중배축신장조절을조절하는 QTLs 을염색체 1 번과 3 번에서탐지하였고 CSSLs 계통을이용하여중배축과토중출아율의상관관계가있음을확인하였다. * 주저자 : Tel , ahnsn@cnu.ac.kr 39

82 OE-11 Hye Jeong Kim 1, Hyun Suk Cho 1, Jae Seong Kim 1, Jin Sol Park 1, Wan Woo Yeom 1, Jae Yong Han 1, Jeong-Il Kim 2, Young Soo Chung 1* 1 2 Plants use light as a source of both energy and information about their environment. Among photoreceptors, cryptochromes are blue-light receptors that regulate multiple light responses including hypocotyl growth, flowering, circadian clock and gene expression in plants and animals. Transgenic soybean plants overexpressing AtKDA1 were produced by Agrobacterium-mediated transformation using the modified half-seed method. The integration of the transgene was confirmed from the genomic DNA of transformed soybean plants using PCR, and the copy number was determined by Southern blotting using leaf samples from T 2 seedlings. To examine the effect of AtKDA1 expression on plant height and its yield in transgenic plants, we compared their growth characteristics to those of non-transgenic (NT) plants. The chosen three transgenic seedlings (#9, #10 and #15) exhibited longer hypocotyls than NT seedlings. Agronomic traits including plant height, the number of nodes per plant, branches per plant, pods per plant and total seed weight were also investigated in GMO field. In phenotypic analysis, AtKDA1 transgenic plants showed longer plant heights than NT plants. *Corresponding Author: chungys@dau.ac.kr OE-12 박현수 *, 백만기, 남정권, 신운철, 김춘송, 이건미, 박슬기, 조영찬, 김보경 벼낟알의크기와형태는쌀의품질과소비자선호도에중요한역할을한다. 우리나라에서육성된대부분의자포니카벼품종은현미장폭비 의단원형이고, 현미천립중 22.0g 내외의중소립특성을나타낸다. 본연구는우리나라자포니카벼품종의협소한입형특성을다양화하기위해벼낟알크기와형태가다양화된육종소재를개발하기위해수행되었다. 입형특성을다양화하기위해극대립자포니카유전자원 Jizi1560 ( 현미천립중 48.1g) 과 Jizi1581 (38.0g) 을우리나라자포니카초다수품종인 드래찬 (24.7g) 과 보람찬 (22.9g) 에각각인공교배한후약배양을수행하였다. 4 조합에서 360 개약배양계통을육성하였고, 이중열악형질수반계통을제외한 290 계통에대해서입형및농업형질특성을조사하였다. 이를통해농업형질이양호하고입형특성이다양화된 91 계통의 입형다양화육종소재 를선발하였다. 입형다양화육종소재 는현미장폭비 , 현미천립중 g 의다양한낟알형태및크기를보유하였다. 입형관련주동유전자인 GW2, GS3, SW5 의유전자형을분석한결과 드래찬 과 보람찬 은 GW2GS3sw5, 대립벼 1 호 는 gw2gs3sw5, Jizi1581 은 gw2gs3sw5, Jizi1560 은 gw2gs3sw5 의대립유전자형을가지고있었다. 입형다양화육종소재 는세개유전자로작성가능한 8 개조합모두가확인되었으며, 유전자형분석을통해입형관련유전자가입형및주요농업형질특성에미치는영향을구명하였다. 중원형입형특성의 DGS77 ( 현미장폭비 2.07) 는 전주 598 호 로명명되어지역적응성시험에공시되었고, 대립특성을가지고있는 DGS8 ( 현미천립중 40.4g), DGS59 (41.5g; gw2gs3sw5) 등은제분효율증진을위한대립형쌀가루품종개발의교배모본으로활용되고있다. 장원형특성의 DGS79 ( 현미장폭비 2.78) 와 DGS83 (2.83; GW2gs3SW5) 은자포니카장원형품종개발의교배모본으로활용되어 DGS79/ 보람찬조합으로부터유망한 HR30971-B-B 계통을육성하여생산력검정시험중이다. 본계통을활용하여입형및내병성에관련된분자표지를활용한 MAS 육종체계로조생, 복합내병성의장원형고품질품종을개발하여쌀수출산업에활용할예정이다. * 주저자 : Tel , mayoe@korea.kr 40

83 OE-13 Rahul Vasudeo Ramekar 1, Neha Samir Roy 2, Youn Sung Kim 3, Kyong Cheul Park 4* and Ju Kyong Lee 1* Transposable elements (TEs) account up to 85% of maize genome and has a significant implication in crop improvement and evolutionary analysis. Mutator (Mu) transposons superfamily, a class of DNA transposons, is the most complex and active element in maize genome suggesting a special role in plant evolution. They can be traced in plants, million years ago since the ancestor of maize and rice diverged. Mu element transposition associates preferentially into or near genic regions causing high mutation rate. These elements can capture host genome sequences contributing to new gene structure. In the present study, we have designed, a set of Mu-specific primers based on terminal invert repeats and utilized a modified transposon insertion display method (Mu-TD) for genotyping. Based on this approach we analyzed the distribution pattern of Mu insertion in teosinte (wild relative), sorghum (distant relative) and few domesticated maize accessions (dent, sweet and waxy). While 17% of the Mu insertion appeared to be fossil insertion shared between teosinte and maize, there is strong evidence of recent activity of Mu element in both the species. Mu-TD analysis suggests the presence of high polymorphic insertion among the species and subspecies indicating the utility of the method in studying genetic variations and species relationship regarding repetitive elements. Further, we analyzed 80 maize RIL population; Mu-TD generated an average of 60% Mu anchored polymorphic fragments where element insertion appeared to be segregating in significantly high number. The amplification profile was highly reproducible exhibiting the utility of Mu elements as a new set of transposon display (TD) markers useful for developing the high-density genetic map. *Corresponding Author: Tel , kyongcheul.park@kangwon.ac.kr Tel , jukyonglee@kangwon.ac.kr OE-14 Junki Lee 1, Shin-Jae Kang 1, Ho Jun Joh 1, Nam-Hoon Kim 2, Sang-Choon Lee 1, Woojong Jang 1, Hyun-Seung Park 1, Jee Young Park 1, Tae-Jin Yang 1,3* Whole genome sequencing (WGS) technologies provide large amount of sequence data. WGS-based molecular markers also have been used in plant research including breeding, barcoding and authentication. Although large number of molecular markers have been developed and actively applied, the development of marker was still limited in non-model plants due to their insufficient genetic resources. Therefore, a simple and universal approach is required to develop molecular markers. In this study, an efficient protocol for assembly complete chloroplast (cp) genome, nuclear ribosomal DNA (nrdna), and polymorphic simple sequence repeat (pssr) which are important to understand genetic diversity and evolution in plant, was designed using the low amount of WGS data. We analyzed cp genome, nrdna, and pssr of Euonymus hamiltonianus, a medicinal and ornamental plant. Using 950 Mb of two WGS data of E. hamiltonianus individuals obtained by MiSeq platform, 157,360-bp complete cp genome, 5,824-bp nrdna, and 162 pssr candidate contigs were identified in E. hamiltonianus. In addition, this protocol was applied to Peucedanum japonicum individuals for pssr identification. Taken together, the protocol designed in this study will help to understand fundamental nature of non-model plants. Acknowledgement: This research was supported by the Bio & Medical Technology Development Program of the NRF funded by the Korean government, MSIP (NRF-2015M3A9A ) and Cooperative Research Program for Agriculture Science & Technology Development (PJ ), Rural Development Administration, Republic of Korea. *Corresponding Author: tjyang@snu.ac.kr 41

84 OE-15 Franz Marielle Nogoy 1, Hye-Jung Lee 1, Marjohn Nino 1, Dal-A Yu 1, Me-Sun Kim 1, Sothea Ouk 1, Yu-Jin Jung 2, Kwon-Kyoo Kang 2, Yong-Gu Cho 1* 1 2 Tryptophan is one of the essential amino acids needed by human bodies and can only be obtained by eating meat, eggs, milk etc. In this study, we harnessed the potential of rice to deliver tryptophan in human diet. Several EMS-mutated rice lines with 30 times higher tryptophan content than their wildtype in rice grains were generated. We characterized the eating and grain quality of these high tryptophan rice lines and found out that they have low eating and grain quality. To improve the rice eating and grain quality, we performed crosses with two elite Korean varieties (Samgwangbyeo and Hopumbyeo). A total of four single cross combinations were forwarded in later generation until we get a stable line with high tryptophan, and good eating and grain quality. Among each population, we performed significant tests for several values on palatability, final viscosity, and amylose to select the best line in each cross combination. In addition, we have also utilized two mutant high tryptophan lines and four breeding lines as wildtypes in transforming CRISPR/Cas9 vectors to regulate the expression of four starch synthesis related genes. Results of this study will enable us to understand the function and correlation of these genes in regulating the amylose content of Japonica rice. *Corresponding Author: ygcho@cbnu.ac.kr OE-16 Yun-A Jeon 1, Dong-Min Kim 2, Hyun-Sook Lee 1, Sun Ha Kim 1, Sang-Nag Ahn 1* 1 2 A high-resolution physical map targeting a cluster of yield-related QTLs on the long arm of rice chromosome 9 was constructed across a 37.4kb region containing the six predicted genes including probable ascorbate peroxidase (OsApx). To analysis of candidate genes, a BC 4 F 6 near isogenic lines (NILs) derived from a cross between the Oryza sativa Hwaseong and O. rufipogon were further evaluated and compared to Hwaseong for nine agronomic traits. Significant difference in plant height was observed between Hwaseong and NILs due to an increase in internodes, particularly in the 1 st and 4 th internode, than Hwaseong. To investigate the antioxidant activity in Hwaseong and NILs, the 3,3-diaminobenzidin (DAB) staining and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay were both conducted. The DAB staining was also performed to understand whether or not OsApx gene is important in scavenging H 2 O 2 in rice. Based on DAB staining, dark-brown color was barely detected in NILs than Hwaseong. Also, the DPPH scavenging ability of NILs showed higher value than Hwaseong. Hence, these results suggested that the internode elongation and antioxidant activity might possibly be controlled by OsApx gene. Acknowledgement: This work was supported by the grant from the Next-Generation Biogreen 21 Program for Agriculture & Technology Development (Project No. PJ ), Rural Development Administration, Republic of Korea. *Corresponding Author: Tel , ahnsn@cnu.ac.kr 42

85 OE-17 Youngjun Mo 1,4*, Hans Vasquez-Gross 1, Luis Alejandro de Haro 2, Tyson Howell 1, Stephen Pearce 3, Jorge Dubcovsky 1,5 1 2 í Despite recent advances in high-throughput sequencing technologies, mapping by whole genome sequencing is still not economically feasible in species with large, complex genomes, such as wheat. We used exome capture to reduce genome complexity and tested a rapid mapping strategy in tetraploid wheat to identify a causal mutation responsible for an increased height phenotype. A mutant progeny population segregating for the mutant phenotype was used for mapping, bypassing the two generations of crossing and self-pollination required for generating an F 2 mapping population. We barcoded individual genomic libraries prior to multiplexed exome capture and sequencing to retain genotype information of each mapping individual. Analysis on induced mutations segregating in the mapping population identified a strong candidate region potentially harboring the causal mutation. Coverage analysis conducted in this region identified 1.9 Mb deletion on chromosome 4BS as the most likely causal variant. Our study provides a rapid mapping approach to identify candidate causal mutations in crop species with large polyploid genome such as wheat. *Corresponding Author: Tel , yjmo@ucdavis.edu 43

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87 한국육종학회포스터발표

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89 PA-01 Young-Jun Park 1*, Kazuhiro Nemoto 2, Kenichi Matsushima 2, Han-Yong Um 1, Jung-Hoon Choi 1, Chan-sung Oh 1 and Tomotaro Nishikawa A gene encoding squalene synthase from grain amaranth was cloned and characterized. The full-length cdna was 1805-bp long and contained a 1248-bp open reading frame encoding a protein of 416 amino acids with a molecular mass of 47.6 kda. Southern blot analysis revealed that the A. cruentus genome contained a single copy of the gene. Comparison of the cdna and genomic sequences indicated that the amaranth SQS gene had 12 introns and 13 exons. All of the exons contributed to the coding sequence. The predicted amino acid sequence of the SQS cdna shared high homology with those of SQSs from several other plants. It contained conserved six domains that are believed to represent crucial regions of the active site. We conducted qrt-pcr analyses to examine the expression pattern of the SQS gene in seeds at different developmental stages and in several tissues. The amaranth SQS gene was expressed late in seed development and played a role in the perisperm, and mainly expressed in stem and root tissues. *Corresponding Author: Tel , ramses11@ekr.or.kr PA-02 Na-Hyun Shin 1, Joong Hyoun Chin 2* and Soo-Cheul Yoo 1,3* Rice is one of the major staple food in Asia, covering around half of the world population. More than 40% of rice cultivation area are subject to abiotic stresses such as drought, submergence and phosphate deficiency. Pyramiding useful genes into elite variety is a promising strategy to develop tolerance varieties to multiple abiotic stresses. However, some genes are not functionally compatible when they are introgressed into the same elite variety. Here, we tested the functional compatibility of Sub1 and Pup1, major QTLs for tolerance to submergence and phosphate (P)-deficiency conditions, respectively. Phenotypic analysis revealed that IR64-Sub1 Pup1(SP1) plants harboring both Sub1 and Pup1 QTLs showed significant tolerance to submerged conditions, similarly in IR64-Sub1 (Sub1) plant, while SP1 plants failed to tolerate to P-deficiency conditions; only IR64-Pup1 (Pup1) showed strong P-deficiency tolerance phenotype. In submerged conditions, the expression levels of Sup1A and PISTOL1, major genes for Sub1 and Pup1 QTLs, respectively, were not significantly different in between Pup1 and SP1 plants. On the other hand, the expression of both Sup1A and PISTOL1 was significantly downregulated in P-deficiency conditions, suggesting that Sub1 and Pup1 repressed gene expression each other in P-deficiency conditions. These results suggest Pup1 does not compromise the Sub1 function in submerged conditions while Sub1 suppresses the function of Pup1, possibly by regulating transcript level of Pup1. In conclusion, Sub1 and Pup1 are functionally compatible in terms of submergence tolerance but not in P-deficiency conditions. Further analysis need to be performed to elucidate how Sup1 suppresses the function of Pup1 in P-deficiency conditions. *Corresponding Author: scyoo@hknu.ac.kr 47

90 PA-03 지희정 1*, 황태영 1, 이기원 1 1 현재우리나라에서풀사료작물로는이탈리안라이그라스가 70% 이상을차지하고있으며종자공급량은약 5 천톤정도로상당히큰비중을차지하고있다. 또한이탈리안라이그라스는지중해연안이원산지로추위에약하지만당도가높아가축기호성및사료가치가좋은것으로나타나수입종자대부분은추위에약해월동전이나월동후이른봄날씨가풀리면서식물체와뿌리가말라서많이동사하여수량감소의원인이되어주로남부지방을중심으로재배해왔다. 따라서국립축산과학원에서는지역별재배시기별맞춤형품종으로총 13 품종을육성하였다. 이들품종은논에서수확시기가빨라이모작재배에알맞은극조생종인그린팜등 3 품종과 5 월상순에수확할수있는조생종인코그린등 3 품종, 봄재배에알맞은중생종인코윈마스터가있으며밭에서여러차례수확이가능한만생종인화산 101 호등 6 품종을육성하여보급하고있다. 이로서재배지가대전이남에서중북부지방으로확대하게되었다. 또한우리품종에대하여해외에홍보를강화하기위하여 2013 년부터미국오레곤주등 11 개소에서국외적응성검정시험을실시하여우리품종의우수성이입증되었다. 따라서종자수입국에서수출국으로변모할수있는계기로 2016 년에는국외기술이전을그린팜등 2 품종에대하여 2016 년부터 5 년간 835 톤미국수출계약을체결하였다. 더불어우리품종을유럽시장에판매하기위한수출기반조성책으로 2016 년도에 EU 에그린팜을품종보호출원을실시하여금년부터현지적응성재배시험중에있다. 또한미국에그린팜품종을품종보호출원을추진하여 2016 년 2 월에국내최초로풀사료작물인그린팜을미국시장에품종등록하였다. 풀사료종자중에서중요한종자품질에대한국제적인인증을얻기위해서국제보증종자인증협회 (AOSCA) 에품종등록을추진하여현재그린팜등 4 품종에대해서도품질인증등록을마쳤다. 또한최근에육성한그린팜 2 호에대해서도 2016 년도부터국외적응성재배시험을추진하고있으며국외품종등록과종자수출계약을지속적으로할계획이며우리나라의남부지방에서현재보다수량이 10% 이상증수할수있는초다수성신품종개발에매진하고있다. * 주저자 : Tel , cornhc@korea.kr PA-04 진성범 1, 박재호 1, 박석만 1, 김민주 1, 김호방 2, 윤수현 1* 1 2 제주도내주로재배되고있는대부분의온주밀감품종들은모계의유전형질을동일하게갖고있는주심배와가지변이등에의하여육성되었기때문에묘목상태로농가에공급된품종들에대하여형태학적으로품종을판별하는것은어렵다. 따라서, 본연구는제주도내재배되고있는온주밀감품종중하례조생품종만을판별할수있는마커개발를목적으로수행하였다. 하례조생품종에특이적인 SNP(single nucleotide polymorphism) 마커 6 조합과 negative control 1 조합을이용하여총 8 품종 ( 하례조생원목, 궁천조생, 흥진조생, 유라조생, 궁본조생, 상야조생, 미택온주, 일남 1 호 ) 의온주밀감중하례조생품종만을선발하기위하여 PCR 를수행하였다. 그결과하례조생품종만특이적으로증폭되는 SNP 마커 2 조합 (P1: HL-SNP-SCAF_ F, HL-SNP-SCAF_ R; P5: HL-SNP-SCAF_ F, HL-SNP-SCAF_ R) 을선발하였고, P1(165bp) 과 P5(526bp) 의 PCR 증폭산물은 1 회의 Multiplex PCR 반응을통하여동시에얻었다. 또한, 주심배실생품종 ( 하례조생 ) 의 SNP 지역은모체인품종 ( 입간조생 ) 과유전적으로변이가있음을확인하였고, 온주밀감 17 품종을재배하는농가에서하례조생품종의판별은 Multiplex PCR 로확인할수있었다. 따라서이 SNP 마커를이용한 Multiplex PCR 방법은하례조생품종을농가에공급함에있어서시간적인단축과경제적인경비를절감할수있을것으로기대된다. * 주저자 : Tel , yunsh04@korea.kr 48

91 PA-05 조성우, 김재덕, 강택규, 백연경, 박철수 * 좋은제빵적성을위해 Glu-B1al ( 과발현 Bx7; Bx7 OE + By8) 대립유전자는매우중요한역할을한다. 이대립유전자는핵산표지인자를이용하여국내재래종밀에서발견되었다. 이대립유전자의 x- 형유전자에의해암호화된글루테닌서브유닛인 Bx7 의과발현을확인하기위하여분자수준의분석을수행하였다. 국내재래종밀 (IT166460) 의 SDS-PAGE 결과는유전적으로 Bx7 OE 지니고있는 Glenlea(Canadian western red spring wheat) 와유사한단백질발현양상을보였다. 이차원전기영동 (two-dimensional electrophoresis) 을이용한분석결과역시 IT 에서 Glenlea 처럼 Bx7 OE 를확인하였다. 고분자글루테닌서브유닛들 (HMW-GSs) 에서 Bx7 의비율을확인하기위하여 RP-HPLC 를수행하였다. IT 의 Bx7 의비율은 56.2 ± 0.2% 로써 CS (Chinese Spring, 34.8 ± 1.0%) 과 Glenlea (46.3 ± 1.8%) 보다높은것으로확인되었다. 유전자중복 (gene duplication) 과프로모터영역의 43 bp indel (insertion and deletion) 은 Bx7 OE 의원인이다. IT 은두요인모두가충족되는것을분석을통하여확인하였다. Bx7 OE 를지닌대부분의밀계통들은주로미국에분포하고있다. 따라서국내밀육종프로그램에있어과발현 Bx7 고분자글루테닌서브유닛을지닌국내재래종밀 (IT166460) 은제빵적성향상을위한유전자원으로써중요한역할을할것으로생각된다. * 교신저자 : Tel , pc89@jbnu.ac.kr PA-06 조성우, 김재덕, 강택규, 백연경, 박철수 * Glu-D1 유전좌위상에서 Glu-1Dy12.K 명명된새로운 y- 형고분자글루텐서브유닛이금강과 CS (Chinese Spring) 과의교배조합 (F 9 ) 에서확인되었다. Glu-1Dy12.K 의특징으로 SDS-PAGE 에서기존의 Glu-Dy12 보다빠른전기영동이동도와낮은분자량이확인되었다. 이차원전기영동을바탕으로한질량분석에서 Glu-1Dy12.K 는 ID: P08488 (GLT3_WHEAT) 인 Glutenin, high molecular weight subunit 12 과높은유사성이이차원전기영동을바탕으로한질량분석을통하여확인되었다. Glu-1Dy12.K 를암호화하는유전자 Glu-1Dy12.K 는 652 아미노산에상응하는 1962 염기서열로이루어져있는것을클로닝을바탕으로염기서열분석을통하여확인하였다. Glu-1Dy12.K 은높은유사성을보이는 Glu-1Dy10 (GI: in NCBI) 과 6 bp 와 18bp 에상응하는두곳의 indels (insertion and deletion) 와 21 SNPs (single nucleotide polymorphisms) 가다른것을확인하였으며, Glu-1Dy12 (GI: ) 와는한곳의 indel 과 3 SNPs 가다른것을확인하였다. Glu-1Dy12.K 에서만 AACAGGACAAGGGCAACA 에상응하는예측된 TGQGQQ 인펩타이드서열이결실된것을확인하였다. * 교신저자 : Tel , pc89@jbnu.ac.kr 49

92 PA-07 Le Thi Dung, Hyun Uk Kim * The Arabidopsis genome contains 16 Indeterminate Domain (IDD) transcription factors. They have divided into two groups with the arrangement of zinc finger motifs at N-terminus and several motifs at C-terminus. In this study, to elucidate the role of IDD7 and IDD11 in plants, identification the mutants and generation of overexpressed plants for IDD7 and IDD11 genes is required. Here we report that IDD7 and IDD11 have a distinct motifs arrangement at C-terminus and have not yet characterized their biological functions. Analyzed amino acid sequence showed two transcription factors have high identical. Loss-of-function of these IDD genes exhibits significantly development with the seedling and seed size are larger than wild-type plants, while double mutants idd7-1 and idd11-1 showed smaller growth. Overexpression of IDD11 plants showed the weak development what belong phenotype inhibitor. Analyzed fatty acid concentration in seeds showed that having a significantly increase in idd7-1 and idd11-1 mutant seeds than their wild-type. Our results provide useful information to detect the function of IDD7 and IDD11 transcription factors in Arabidopsis thaliana. *Corresponding Author: Tel , hukim64@sejong.ac.kr PA-08 Joo-Seok Park 1, Chaeyoung Lee 2, Jin-Hyun Kim 2, Yoram Choi 1, Min-Gyun Jeong 1 and Hong-Kyu Choi 3* Genome-wide transcriptome analysis is one of the most powerful means to gain a broad and deep insight into the molecular mechanisms that underlie dynamic interactions among numerous genes in organisms. Although several bioinformatic platforms for gene expression profiling have been developed for individual species, platform for cross-species transcriptome analysis is not currently available. To develop such a bioinformatics system, we employed the technical concept of translational genomics between different species and aimed to build the platform in user-friendly manner. This DB- linked platform, named LegExpress, harbors wide array of transcriptome data for three representative species with relatively the most comprehensive gene expression information, including Glycine max, Medicago truncatula and Arabidopsis thaliana. All these expression data were collected from publically available ArrayExpress ( DB and composed mainly of Affymetrix GenChip data. Raw data were processed to select high-quality transcriptome data and normalized by the RMA standardization method. We developed a program for visualization of the data and organized the user interface according to suitable criteria, such as organs, developmental stages, time courses and different stimuli (e.g., hormones, biotic/abiotic stresses). It is anticipated that LegExpress may play a useful role for breeder/researcher-friendly transcriptome analysis platform and can be applied to design breeding programs through helping breeders discover trait-associated genes. *Corresponding Author: Tel , hkchoi@dau.ac.kr 50

93 PA-09 Jin-Hyun Kim 1, Chaeyoung Lee 1, Joo-Seok Park 2, Yoram Choi 2, Min-Gyun Jeong 2 and Hong-Kyu Choi 3* Comparative genome analysis is a powerful approach to look into the genomic organizations among different, but evolutionary related, species, to predict function of certain genes of interest and to interpret evolutionary relationships between compared species. For such reasons, development of efficient and automated bioinformatic visualization tool is essential in this research field. To achieve this goal, we intended to construct an interactive and flexible bioinformatic interface for the comparative analysis focused on legume genomes, named LegCompara. This platform consists mainly of two parts: a web-based user interface and corresponding relational databases. The database harbors a diverse array of genomic information (e.g., functional annotation, ortholog groups) for seven legumes (M. truncatula, G. max P. vulgaris, C. cajan, V. radiata, C. arietinum) and two model plants (A. thaliana, O. sativa). This genome browser was developed by employing the JavaScript and SVG (Scalable Vector Graphics)-based programs. As a result, we could significantly reduce server s workload, gain rapid response, and enhance graphic visibilities of the interface. This system, unlike other traditional genome browsers, was designed for researchers to dynamically interact with user interface, so it can navigate multiple chromosomes of different or same species simultaneously, resulting in genome-wide and/or regional comparisons by depicting corresponding syntenies with either blocks or lines between orthologous regions or genes. Instant transition of micro-to-macro synteny analyses are also possible by zooming in/out from individual gene to full chromosome scale. It is expected that LegCompara may provide researchers and breeders with useful resources for more efficient and user-friendly comparative genome analysis. *Corresponding Author: Tel , hkchoi@dau.ac.kr PA-10 Kitae Song 1, Hyochul Kim 1, Kyung-Hee Kim 1, Jun-Cheol Moon 2, Jae Yoon Kim 3, Byung-Moo Lee 1* Drought stress is an important factor determining yield, growth and seed quality in maize. We have analyzed drought related genes and their alternative splicing which can make change tolerance using RNA-sequencing and bioinformatics tools. We identified a total of 723 genes and 235 transcripts whose expression was changed by drought in maize. The genes and isoforms were classified by blast, and we also found a multitude of possibilities to estimate correlation between drought and gene function. Furthermore, we identified several drought responsive transcript factors containing zinc finger and NAC domains. The transcript factors are frequently involved in gene expression, included gene regulation and alternative splicing. Overall, our results provide a genome-wide analysis of differentially expressed genes (DEGs), novel transcripts, and alternative splicing under drought stress. Further characterization of the drought-responsive transcripts and their expression patterns identified in this study has potential to advance our understanding of drought tolerance in maize. Acknowledgment: This work was carried out with the support of Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ ) Rural Development Administration, Republic of Korea. This research was supported by Administration, Republic of Korea, the Golden Seed Project ( SB710), Ministry of Agriculture, Food and Rural Affairs (MAFRA), Ministry of Oceans and Fisheries (MOF), Rural Development Administration (RDA), and Korea Forest Services (KFS). *Corresponding Author: Tel , bmlee@dongguk.edu 51

94 PA-11 Woo-Jae Kim *, Jae-Beom Chun, Hyun-Su Park, Hyun-Soon Kim, Kyung-Ho Kang, Ji-Ung Jeong, Jae-Kwon Ko This study was carried out to develop rice variety integrated with rice bacterial blight resistance gene. Advanced Backcross Lines (ABLs) 5 that contains Xa1, Xa3, xa5, and Xa21, was developed from backcross 2 times between Japonica cultivar Hopum having bacterial blight resistant Xa3 gene and Indica cultivar VND95-20 having Xa1, xa5, and Xa21 gene and disease resistance bioassay and marker-assisted selection. Advanced Backcross Lines(ABLs) 21 that contains Xa3 and Xa21, was developed from backcross 2 times between Japonica cultivar Hanggeumnuri having bacterial blight resistant Xa3 gene and Indica variety IRBB21 having Xa21 gene and disease resistance bioassay and marker-assisted selection. Reaction of ABL5-1 and ABL21-1 against 18 isolates in K3a race showed stable durable resistance reaction and lesion length shorter than the donor parents. Analysis of variance conducted for confirm uniformity in pathogenicity of isolates and repeat values of lesion length to each isolates were not significant at p=0.05. In result of cluster analysis, HB4032 isolate showed very high pathogenicity among 18 isolates. *Corresponding Author: Tel , suwonman@gmail.com PA-12 Sampathkumar Palaniswamy 1, Jagadeesh Sundaramoorthy 1, Gyu Tae Park 1, Jeong-Dong Lee 1, Hak Soo Seo 2, Jong Tae Song 1* 1 2 From the time when agriculture began, food grains undergo various selections and breeding for size as well as for other attribute. These phenotypic characters are governed by the genetic makeup. Soybean [Glycine max (L.) Merr] is one of the major agricultural crop mostly used for food and oil. 100 seed weight is the common method to measure the seed size of soybean. For accuracy, measurements like seed length, seed width, seed thickness and seed volume are also added. With the development of molecular biology techniques, marker assisted breeding and advanced genetic sciences it is possible to uncover the genetic makeup involved in the seed size. Over three decades many Quantitative Trait loci (QTLs) are identified for improving this trait. But still the allelic genes responsible for larger seed size and smaller seed size are unknown. In search of genetic resource involved in this trait, we developed an Ethyl methanesulfonate (EMS) mutant line PE887 with smaller seed size. In addition segregating population was developed by breeding cross between PE887 and Pungsannamul. Further genetic linkage mapping will be carried out to find gene(s) involved in the determination of seed size. Acknowledgement: This work was carried out with the support of Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ ) Rural Development Administration, Republic of Korea. *Corresponding Author: Tel , jtsong68@knu.ac.kr 52

95 PA-13 Thi Hai Yen Nong 1, Jagadeesh Sundaramoorthy 1, Gyu Tae Park 1, Jeong-Dong Lee 1, Hak Soo Seo 2, Jong Tae Song 1* 1 2 Phosphorus (P) is a nutrient essential for growth of plants that is available in composition of the nucleic acids, ATP, coenzyme. Phytic acid (myo-inositol-1,2,3,4,5,6-hexakisphosphate) is the main storage form of phosphorus in yeast, mammalian and almost economically important crops. In soybean [Glycine max (L.) Merr] seeds, phytic acid is found in the form of phytate salt mixtures with multivalent cations, proteins, Ins P and starch that prevent their assimilation through the digestive system. Thus, a large amount of phosphorus in the form of phytate undigested monogastric animals and excreted into environment. The objective of the present study is to identify soybean mutants with high available P. Inorganic P was content in 1,266 ethyl methanesulfonate (EMS) M 4 lines from the cultivar Pungsannamul. As the result of our study, we isolated four EMS lines (PE379, PE432, PE2205 and PE2503) with high inorganic P ( g kg -1 ) and six EMS lines (PE718, PE828, PE1466, PE1552, PE3378 and PE3386) with low inorganic P ( g kg -1 )(compare with control line Pungsannamul: 0.90 g kg -1 ). In present, we are selected homozygous of HIP mutant line (PE432). In the future, we will continue selection of homozygous LIP mutants and carried out breeding cross between mutant lines and wild controls lines in order to find the gene(s) responsible for the low and high inorganic P phenotypes. Acknowledgement: This work was carried out with the support of Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ ) Rural Development Administration, Republic of Korea. *Corresponding Author: Tel , jtsong68@knu.ac.kr PA-14 Juhyun Im 1, Woon Ji Kim 1, Sang Hun Kim 1, Seung Cheol Oh 2, Lan Cho 2, Si-Yong Kang 3, Sung-Hwan Jo 4, Bo-Keun Ha 1* Boysenberry is one of the genus Rubus and derived from a cross between Loganberry and a trailing blackberry. Recently, several new boysenberry and blackberry genotypes were developed from mutation breeding technology. In this study, we evaluated genetic diversity among 10 boysenberry genotypes and 6 blackberry genotypes using SNP markers. These SNP markers were identified by genotyping-by-sequencing (GBS) technology. GBS libraries were constructed using 16 different barcodes and the restriction enzyme ApeKI (GCWGC). SOAPdenovo2 produced the highest number of contigs at a k-mer length of 31 and the assembled contigs were used as references. We identified a total of 23,529 polymorphic SNPs and 464 InDel among 16 boysenberry and blackberry genotypes. After filtering SNPs with missing rates < 30%, 1,504 SNPs were used to built a phylogenetic tree among 16 genotypes. In addition, we identified 24 SNP marker set discriminating 16 boysenberry and blackberry genotypes. This SNP marker set will allow for the rapid identification of Rubus germplasm. *Corresponding Author: Tel , bkha@jnu.ac.kr 53

96 PA-15 Sang Hun Kim 1, Juhyun Im 1, Woon Ji Kim 1, Ryuli Gang 1, Si-Yong Kang 2, Bo-Keun Ha 1* 1 2 Soybean [Glycine max (L.) Marr.] seeds are the most valuable sources of vegetable oil. However, the partially hydrogenated soybean oil desirable for solid fat baking applications creates trans fats associated with negative health effects on blood lipids. The chemical hydrogenation can be reduced by increasing stearic acid content that is a saturated fatty acid and neutral effect on cholesterol level. This study identified new mutant genotype containing ~17% stearic acid. This mutant genotype was developed from gamma irradiation treatment with original cultivar, Ilmi containing ~3% stearic acid. The mutant contained a three base pair deletion in the 1 st exon of stearoyl-acyl-carrier protein desaturase (SACPD-C) gene. The deletion of three bases resulted in a protein that is missing one amino acid. This mutation could affect the function of SACPD-C gene and elevate seed stearic acid levels. *Corresponding Author: Tel , bkha@jnu.ac.kr PA-16 이주영, 김용호 * 뼈형성단백질 (Bone morphogenetic protein, BMP) 은세포조절인자로현재까지최소 20 종류가보고되었다. 본연구에서는인간 BMP-2 유전자를일미벼에형질전환하고도입유전자의발현수준을전사체와단백질수준에서확인하였다. hbmp-2 유전자를 ppzp200 vector 를사용하여 Agrobacterium 을매개로벼캘러스에도입시킨형질전환체에서 total RNA 를추출하여 cdna 를합성한뒤 Quantitative real time-pcr(qrt-pcr) 을수행하였다. 벼의내재유전자로사용된 actin 과도입유전자인 hbmp-2 의비특이적인증폭여부를확인하기위해 melt curve 를분석한결과대조구인일미벼를제외한나머지시료에서 melting temperature 가 83 로모두일정하게나타났다. 전사체의상대적인 mrna 량은기준량대비 배가발현됨을확인하였으며, 또한 western blot analysis 를통하여형질전환체에는도입유전자에의한단백질이형성되었음을확인하였다. * 주저자 : Tel , yohokim@sch.ac.kr 54

97 PA-17 Woon Ji Kim 1, Juhyun Im 1, Sanghun Kim 1, Bo-Keun Ha 1* 1 Ionization radiation causes DNA single strand breaks (SSB), double strand breaks (DBS), damage or loss of bases, and intramolecular / intermolecular crosslinking. Since there is no report on the mutation rate according to the proton beam irradiation in soybean, the present study investigates the genetic variation in the soybean chromosome induced by the proton beam irradiation. Total of 22 plants including each 10 M 2 plants induced from 118Gy and 239Gy of proton beam, respectively, and 2 wild-type plants (Deapungkong) were sequenced using GBS technology. Total of 7,453 SNPs were observed in proton beam irradiated M 2 plants. Of these, 3,569 SNPs were observed in the genic regions. Among 5,829 union SNPs, transition and transversion were 47% and 53%, respectively. The highest substitution ratio was for transversion A / T with 37%. Most SNPs (82%) were distributed in one gene, while the rest (12%) had 2 to 5 SNPs per gene. The SNPs induced by proton beam were uniformly distributed in most of the chromosomes. This study will establish a framework for constructing a pool of mutant genetic resources using the proton beam. *Corresponding Author: Tel , bkha@jnu.ac.kr PA-18 Woon Ji Kim 1, Juhyun Im 1, Sanghun Kim 1, Ju Seok Lee 2, Sungtaeg Kang 3, Soon-Chun Jeong 4, Jung-Kyung Moon 5, Bo-Keun Ha 1* In this study, we conducted a genome-wide association study (GWAS) between genetic variation and amino acid contents in core collection of wild soybean. The 203 accessions were used for analyzing amino acids with an amino acid auto-analyzer (S433-H, SYKAM). The total amino acid contents of 203 accessions ranged from 32,896 to 53,251 (mg/100g). The content of cystine was ~ (mg/100g) and the content of methionine was ~ (mg/100g). The correlation between cystine and methionine was r = **, and the 17 amino acids showed highly significant and positive correlation with each other. By GWAS with 130K SNP genotyping data, AX SNP marker showed significant association with three amino acids such as glutamic, leucine, and phenylalanine. The AX was located in 47,702,857 bp of chromosome 9. Four genes, Glyma.09g257300, Glyma.09g258000, Glyma.09g249100, and Glyma.09g250700, associated with amino acids were identified near the genetic region of AX Our results will help us to understand the genetic basis of amino acid biosynthesis in soybean seeds. *Corresponding Author: Tel , bkha@jnu.ac.kr 55

98 PA-19 서정현 1*, 강범규 1, 김현태 1, 신상욱 1, 김홍식 1, 백인열 1, 한원영 1, 윤홍태 2, 이병원 2, 고종민 3, 곽도연 검정콩은맛이좋고무름성이좋아예로부터밥밑용으로사용되어왔으며최근에는종피에들어있는기능성물질에대한관심이높고연구가활발하다. 이러한검정콩은건강기능성식품으로서소비가증가하고시장가격이높게형성되어농업인들의수요가늘고있지만도복, 탈립등재배형질이불리하여이에대한개량이필요하다. 소비자들은검정콩의자엽색이푸른것을선호하며용도확대를위하여다양한크기의품종개발이요구된다. 태청 은대립이면서녹자엽인검정콩육성을목표로하여 YS1593( 밀양 136 호 단파흑대두 ) 을모본으로, 청자 2 호 를부본으로 2005 년에인공교배하였으며계통육종법에의해 YS1764-2B 이선발되었다. 12 ~ 13 년도에실시한생산력검정시험에서는극대립녹자엽계통으로유망시되어 밀양 278 호 의계통명을부여하고 14 ~ 16 년 3 년간 7 개소에서지역적응시험을실시하였다. 태청 은유한신육형으로서엽형이난형, 꽃색이자색, 모용색이갈색, 종실은구형으로검은색종피에자엽색이녹색인고유특성을가지고있다. 성숙기는 10 월 20 일로청자 3 호보다 3 일늦으며 100 립중은 44.5g 으로청자 3 호에비하여 10g 더무겁다. 지역적응시험에서 태청 의내도복성은청자 3 호와비슷한수준으로강하였고, 협개열성은청자 3 호보다강하였다. 태청 의수량은지역적응시험 7 개소평균 240kg/10a 로청자 3 호 (254kg/10a) 의 94% 수준이었다. 이처럼 태청 은알이굵으면서도도복과탈립에강하여재배특성이좋고우수한외관품질을가지고있으므로콩초콜릿등가공용 ( 제과용 ) 및밥밑용콩으로서널리사용될것으로기대된다. * 주저자 : Tel , next0501@korea.kr PA-20 Shi Jun Ma, Kyu Jin Sa, Tak-Ki Hong, Ju Kyong Lee * In this study, 21 simple sequence repeat (SSR) markers were used to evaluate the genetic diversity and population structure among 77 Perilla accessions from high-latitude and middle-latitude areas of China. 95 alleles were identified with an average of 4.52 alleles per locus. The average polymorphic information content (PIC) and genetic diversity (GD) values were and 0.372, respectively. The level of genetic diversity and PIC value for cultivated accessions of var. frutescens from middle-latitude areas were higher than accessions from high-latitude areas. Based on the dendrogram of unweighted pair group method with arithmetic mean (UPGMA), all accessions were classified into four major groups with a genetic similarity of 46%. All accessions of cultivated var. frutescens were clearly discriminated from the cultivated var. crispa. Furthermore, most accessions of cultivated var. frutescens collected in high-latitude and middle-latitude areas were clearly distinguished depending on their geographical location. However, the geographical locations of several accessions of cultivated var. frutescens have no relation with their positions in the UPGMA dendrogram and population structure. This result implies that the diffusion of accessions of cultivated Perilla crop in the northern areas of China might be through multiple routes. On the population structure analysis, 77 Perilla accessions were divided into Group I, Group II, and an admixed group based on a membership probability threshold of 0.8. Finally, the findings in this study will provide useful theoretical knowledge for further study on the population structure and genetic diversity of Perilla and benefit for Perilla crop breeding and germplasm conservation. *Corresponding Author: Tel , jukyonglee@kangwon.ac.kr 56

99 PA-21 Muhammad Qudrat Ullah Farooqi, Shi Jun Ma, Kyu Jin Sa, Ju Kyong Lee * In this study, maize inbred lines were used to evaluate performance under drought stress. Based on germination ability, 24 lines were screened out with tolerance or susceptibility to drought stress. Genetic diversity, relationship, and population structure were evaluated by using 100 SSR molecular markers linked with drought tolerance in maize. A total of 465 alleles were identified with an average of 4.65 alleles per locus using bulk segregant analysis (BSA). Out of them, 199 were specific alleles with tolerance and 35 were susceptible to drought, while the remaining 231 were shared between the two types. The major allele frequency varied from 0.39 to 0.61 with an average of The gene diversity (GD) and polymorphic information content (PIC) average values from all lines were 0.64 and 0.59, respectively. Based on UPGMA analysis, four main cluster groups were identified as most of the drought tolerant lines were clearly discriminated from drought susceptible lines with 33% genetic similarity. On population structure analysis, 24 inbred lines with tolerance and susceptibility were divided into 3 groups. In addition to BSA, a total of 88 SSR s were identified as specific to tolerance and 29 SSR markers were specific to susceptible lines. Among these, bnlg1627, umc1946, dupssr30b, bnlg1812, and dupssr24 SSR markers had great potential for the improvement of drought resistance in maize. Our results were in good agreement with previous studies for drought tolerance using SSR molecular marker and will be useful for further maize breeding studies. *Corresponding Author: Tel , jukyonglee@kangwon.ac.kr PA-22 Rahul Vasudeo Ramekar 1, Kyong-Cheul Park 2, Kyu Jin Sa 1, Ju Kyong Lee 1* 1 2 Genetic Linkage map can provide a deeper understanding of random markers distribution along the chromosome which can be employed for a range of plant breeding objectives. Transposons constitute a large fraction of eukaryotic genome and can have a high impact on overall genome structure. We have designed a set of Mutator (Mu) specific primers based on terminal invert repeats and utilized a modified transposon insertion display method (MU-TD) for genotyping. Here we describe the chromosomal distribution of Mu markers added to a linkage map of maize using anchored SSR markers. The mapping population included 80 F 7:8 RILs derived from cross between dent corns and waxy corn, evaluated for ten grain yield and kernel quality related traits over 2 year period. The TD analysis produce a high profile of the polymorphism between the parental lines with 20 Mu- specific primers. Number of polymorphic bands ranged from 5 to 33. The linkage map included 258 Mu-anchored fragments along with 614 SSR markers distributed along 10 chromosomes and covered a span of cm. The integration of Mu loci extended the map in several telomeric regions and reduced several gaps present in previous map. A total of 22 QTLs were confirmed in 2011 and 2012 for various traits which included total of 18 Mu loci. This work represents an important step forward in genomics and marker assisted breeding in maize. *Corresponding Author: Tel , jukyonglee@kangwon.ac.kr 57

100 PA-23 Rahul Vasudeo Ramekar 1, Kyong-Cheul Park 2, Kyu Jin Sa 1, Ju Kyong Lee 1* 1 2 In Gangwon Agricultural Research and Extension services at the Maize experiment station a large collection of maize inbred lines (waxy and common) of diverse origins, both from local farmers and other countries are assembled. Waxy maize has originated from cultivated flint maize and most recently underwent divergence from common maize. Thus understanding genetic variation and differentiation in this elite lines is fundamental for selection, by which progress in plant breeding can be achieved. Transposable elements (TEs) account up to 85% of the maize genome, creates a large and stable insertions in the genome generating variations which reflect selection acts during evolution and plays a significant role in genome organization, yet they have not been used in population genetics and association analysis. We recently developed a set of Mutator specific primers based on terminal invert repeats and utilized a modified transposon insertion display method (MU-TD) for genotyping. Using this method we studied genetic diversity and population structure of 40 waxy and 40 common maize. Also to explore the contribution of mutator T.Es to phenotypic variation, we undertook association analysis in the same maize line phenotype by ten economical important traits. The results revealed substantial genetic diversity in the studied panel, and that genetic variation in waxy maize was lower compared to common maize. Principle co-ordinate analysis, neighbor-joining cluster analysis, and STRUCTURE analysis supported the presence of two genetically distinct groups. Analysis of molecular variance revealed significant genetic differentiation within the population than among the population describing genetic variation mainly resides within the population. We also found a highly significant association between seven mutator loci with four different traits. These results will be useful for marker-assisted selection in maize breeding program to help breeders choose parental lines and markers for crossing as well as in understanding the role of transposons in creating diversity and shaping the evolution in maize. *Corresponding Author: Tel , jukyonglee@kangwon.ac.kr PA-24 박노봉 1*, 남민희 1, 김상열 2, 여운상 2, 이지윤 2, 권오덕 1, 박동수 2, 이종희 3, 조준현 2, 송유천 2, 오성환 국립식량과학원영덕출장소에서는최근의이상기상에따른병해충증가에대응하기위해밥맛과재배안정성이뛰어난고품질벼품종을육성하고자노력한결과, 우리나라중부및영남평야지와동해안냉조풍지에알맞은고품질중생종벼 상보 를개발하였다. 모본으로재배안전성이뛰어난 영덕 32 호 ( 삼덕벼 ) 를, 부본으로는도정율이우수한 남평벼 를 2002 년하계에인공교배하여 2003 년하계에 32 개체의 F 1 식물체를양성, YR24256 의교배번호를부여하였다 년하계포장에전개한 F 2 집단을포장과실내선발하여 2005 년하계에 F 3 세대 142 계통을육성하였다. 이후 F 4 F 8 세대를계통육종법으로전개하면서예비선발시험을실시하여미질특성이우수하고병해와재해에안정적인계통을선발하였다 년생산력검정예비시험, 년생산력검정본시험을실시한후중생이면서우량계통인 YR 을선발하여 영덕 59 호 로명명하였다 년부터 3 년간지역적응시험을실시한결과, 복합내병성이면서특히숙색이양호한것으로평가되어 2015 년 12 월농작물직무육성신품종선정위원회에서 상보 로명명하게되었다. 상보 는보통기재배시출수기는 8 월 9 일로중생종이며, 간장은 77cm 로약간큰반직립초형이다. 탈립은잘되지않고이삭추출은양호하며까락이거의없다. 화성벼 보다수당립수는 19 개더많으며, 현미천립중도 24.1g 으로더무겁다. 쌀알이맑고투명하며, 도정특성과밥맛도 화성벼 보다우수하다. 또한불시출수는안되는편이고, 위조현상에강하고성숙기엽노화가느린편이며, 내냉성은 화성벼 보다약한중약정도이다. 잎도열병에중정도의저항성을보였고, 줄무늬잎마름병, 흰잎마름병 (K 1, K 2, K 3 ) 에는강하나오갈병, 검은줄오갈병에는약하고, 벼멸구등충해에는감수성이다. 쌀수량은지역적응시험보통기재배 9 개소에서 6.21MT/ha 로 화성벼 보다 11% 증수되었으며이모작적응성도높아적응지역인중부및영남평야지, 남부중산간지, 동남부해안지에보급하게되었다. * 주저자 : Tel , parknb@korea.kr 58

101 PA-25 Mi Jeong Jeong *, Joo Yeol Kim *, Soo In Lee and Jin A Kim We previously reported that sound wave treatment (1 khz) delays fruit ripening in tomato (Solanum lycopersicum), affecting the expression of ethylene biosynthesis-related genes encoding 1-aminocyclopropane 1-carboxylic acid (ACC) synthases (ACS) and ACC oxidases (ACO). In this study, we investigated the activity of the transcription factors RIN and HB-1, which function in the ethylene biosynthetic pathway, in response to sound treatment. To investigate whether RIN and HB-1 directly activate the transcription of ACS and ACO, we performed transcriptional activation analysis in Arabidopsis thaliana leaf protoplasts, transiently expressing RIN or HB-1 and using reporter constructs with promoters of the tomato ACS and ACO genes. Activation of the endogenous AtACS and AtACO genes was also measured by qpcr. The RIN- and HB-1-induced expression of these genes decreased, but the HB-1-induced expression of some genes increased after sound treatment. To confirm these results, we performed transient assays in Nicotiana tabacum, which produced results similar to those observed in Arabidopsis. The major ethylene biosynthesis-related genes harbor a CArG-box as a RIN-binding motif. These findings indicate that RIN and HB-1 affect the expression of ethylene biosynthesis-related genes in response to sound treatment, and they suggest that RIN may regulate the ethylene biosynthesis-related genes by binding to their CArG-boxes. *These authors contributed equally to this work. Author center1097@korea.kr PA-26 Yong-Sam Kwon 1, Norvie L. Manigbas 2, Do-Hon Kim 1 and Gihwan Yi 3* Phylogeny of 246 Korean rice varieties from 3 breeding institutes were evaluated using 26 SSR primers. Two hundred ninety alleles were detected on 11 chromosomes. The number of alleles detected per locus ranged 7-16, with an average of alleles per locus. The highest frequency of allele occurrence was 22.1% on chromosome 6 and the lowest frequency was 1.7% on chromosome 11. The polymorphic information content (PIC) values ranged with an average of Higher PIC values suggests that primer sets could be used in identifying Korean rice varieties. Genetic similarity analysis revealed two major groups. Group 1 included most of the japonica varieties, and Group 2 included predominantly the tongil type rice which is a hybrid of indica and japonica. Cluster analysis showed that there were no distinct clusters among breeding institutes however, some varieties from the same breeding institute occurred in the same sub-cluster. Rice varieties released by the Department of Rice and Winter Cereal Crop exhibited the greatest genetic differences, whereas those of the National Institute of Crop Science exhibited the lowest among the breeding institutes. The phylogenic data suggests that genetic diversity of varieties released by National Institute of Crop Science was greater than that of Department of Rice and Winter Cereal Crop and the Department of Functional Crop. Genetic diversity of special purpose rice such as aromatic, pigmented, waxy, and super yield was greater than rice grown for premium quality. Phylogenic analyses provide a reference data in determining parents to cross for genetic improvement. *Corresponding Author: Tel , gihwan@knu.ac.kr 59

102 PA-27 Jin-Kee Jung *, Eun-Jo Sim, Seul-Ki Lim, Dong Min Kim and Seung-In Yi The construction of a DNA database for identify the Sesame(Sesamum indicum) has been made possible by the development of novel single nucleotide polymorphism(snp) markers. For the development of novel SNP marker, out of 123 varieties, 71 varieties (38 application for protection of new varieties of plants and 33 registration for production and import) that are registered at the Korea Seed & Variety Service for Plant Variety Protection (PVP) and 52 imported varieties were used in this study. Finally 48 SNP(include 7 InDel) loci derived from EST region were tested for develop SNP marker for varieties identification. Finally, 27 SNP markers based on EST were developed for identification of 127 Sesame (Sesamum indicum) and constructed of a DNA database. Totally 119 varieties (67 Korean cultivar and 52 imported cultivar include China, Ethiopia, India, Sudan and Uzbekistan) were identified using these markers except 4 Korean varieties (Alibaba/Superstar, Yeolyeora/Supertaegang). Average polymorphism information content (Shannon s information index) was 0.553, ranging from to And we selected highly informative 12 SNP markers and construed multiplex systems for effective application to Sesame variety identification for Korean cultivars. These highly informative SNP markers will useful for variety identification related to seed dispute and distinctness, uniformity and stability (DUS) test and origin identification for Sesame. Acknowledgement: This work was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries(IPET) through Agri-Bio industry Technology Development Program, cunded by Ministry of Agriculture, Food and Rural Affairs(MAFRA) ( ) *Corresponding Author: Tel , jinkeejung@korea.kr PA-28 Songhwa Chae 1, Joung Sug Kim 1, Kyong Mi Jun 2, Sang-Bok Lee 3 and Yeon-Ki Kim 1* Rice (Oryza sativa) has evolved under a tropical climate and expanded its growth area into temperate zones through the domestication by human. Accordingly flowering and cold resistance are main agronomic traits to guarantee for stable crop production in the area. Efforts to find quantitative trait loci related to these traits have intensified with the next generation sequencing technology. We have developed 400 recombinant isogenic lines (RILs) using Jinbuolbye (JBO) as a female and Samgwang (SG) as a male. JBO is cold stress tolerance and heading at Jul. 12 while SG is cold sensitive and heading at Aug. 15. SG has a high seed quality and been commercialized in Korea. These RILs have currently iterated over 6 generations and the traits related to panicle development and cold resistance been documented. Around 12 gb (40 x genome) have been sequenced for parents and 2 RILs, respectively to provide saturated markers based on single nucleotide polymorphisms (SNPs). The phylogenic relation has been analyzed with snphylo showing the relationship between parental and 2 RILs. Mutations in genic and intergenic regions are further analyzed with SnpEff. Around 590,000 variants are detected among the lines across the chromosomes. The significance of the SNPs related to agronomic traits are discussed. *Corresponding Author: kim750a11@gmail.com 60

103 PA-29 Il-Sheob Shin 1,3*, Jaean Chun 2 and Yoon-Kyeong Kim The molecular understanding of pears against scab caused by Venturia nashicola, a most detrimental fungal disease to pear fruit production worldwide, is very limited. Comparing resistant line [93DB, interspecific hybrid between Danbae (Pyrus pyrifolia P. ussuriensis) and Bartlett (P. communis)] with susceptible cultivar Sweet Skin (SS, P. pyrifolia) according to seven time points of 0, 0.5, 1, 2, 3, 4, 8 days post inoculation (DPI), transcriptomes derived from infected and mock-inoculated young leaves were obtained through Illumina sequencing and analyzed to evaluate the tolerant response and to mine candidate genes of pear to the scab pathogen. Analysis of the mapped reads showed that the infection of V. nashicola led to significant differential expression of 17,827 transcripts with more than 3-fold change in the seven pairs of libraries, of which 9,672 (54%) are up- and 8,155(46%) are down-regulated. These included mainly receptor (NB-ARC domains-containing, CC-NBS-LRR, TIR-NBS-LRR, seven transmembrane MLO family protein) and transcription factor (ethylene responsive element binding, WRKY DNA-binding protein) related gene. To identify transcripts specifically expressed in response to V. nashicola, RT-PCRs were conducted and compare to the expression patterns of genotypes with a range of highly resistant to highly susceptible symptom. An arsenal of defense response of highly resistant pear accessions derived from European pear was probably supposed no sooner had V. nashicola infected its host than host genes related to disease suppression like Polyketide cyclase/dehydrase and lipid transport protein, WRKY family transcription factor, lectin protein kinase, cystein-rich RLK, calcium-dependent phospholipid-binding copine protein were greatly boosted and eradicated cascade reaction induced by pathogen attack within 48 hours. Moreover transcripts activating defense response to V. nashicola were assumed to be triggered by stronger and faster signaling of ethylene and/or jasmonic acid as a stimulus for defense priming to pear plant. This study provides the first transcriptional profile by RNA-seq of the host plant during scab disease and insights into the response of tolerant pear plants to V. nashicola. *Corresponding Author: il-sheob.shin@worldveg.org PA-30 Man-Kee Baek 1, Jong-Min Jeong 1, Hyun-Su Park 1, Soon-Wook Kwon 2, Woo-Jae Kim 1, Jeong-Kwon Nam 1, Seul-Gi Park 1, Choon-Song Kim 1, You-Chun Song 1, Bo-Kyeong Kim 1, Young-Chan Cho 1* 1 2 Rice blast caused by the fungal pathogen Magnaporthe oryzae is a destructing disease of rice that poses serious threats to the world s food security. The use of blast resistant variety of rice is known as the most effective and economical method of controlling this disease. The resistance genes are rapidly been overcome by the new pathogens to be differentiate race. The quantitative trait loci (QTL) is stable against different pathogenic races of the rice blast fungus; hence, the use of QTL is one of the most promising measures for blast control. This study is to identify QTL related to durable resistance in a japonica variety, Palgong that has showed resistance to blast since Palgong was crossed with a susceptible japonica variety Ilpum and F1 was backcrossed with Palgong. A BIL population was developed from the resultant BC1F1 plants by the single-seed descent method. A BIL population was genotyped by Illumina array analysis 768 SNP of japonica rice genetic background (Nagasaki et al. 2010). 216 SNPs except for SNPs of monomorphic and over 10% of missing data were constructed to linkage map and analyzed to QTL for the data of blast nursery screening. In CIM analysis, the putative QTLs were over 2.52 of an average LOD in P<0.05 and over 3.80 of an average LOD in P<0.01, respectively. There were detected 16 putative QTLs explaining % of phenotype variation on chromosomes 2, 4, 7, 11 and 12. The major QTLs were qpbn2.1, qpbn2.3, qpbn4.1, qpbn4.2, qpbn7.2, qpbn11.1, qpbn11.2 explaining % of phenotype variation. *Corresponding Author: Tel , yccho@korea.kr 61

104 PA-31 김정주 1*, 신운철 2, 김우재 3, 박현수 3, 강위금 1, 남정권 3, 백만기 3, 조영찬 3, 김보경 3, 김대식 조생종벼품종은주로중산간지역에서재배되어왔으나평야지에서도추석전햅쌀조기출하를목적으로조기재배면적이증가하고있다. 따라서조생종벼재배면적을늘림으로써쌀출하시기조절및노동력분산효과를기대할수있다. 중산간지적응복합내병성고품질조생종벼신품종 성산 은 2008/2009 년동계에복합내병성조생계통인운봉 42 호와중산간지적응도열병저항성계통인상주 40 호 (IT242653) 를인공교배하여교잡육종법으로육성되었다. 인공교배후분리세대인 F 2 집단에서초형및이삭특성을고려하여포장선발하고 F 3 이후부터는계통으로전개하여주요병해충및미질특성을고려하여선발하였다. 그중조생종이면서복합내병성인계통으로선발된 HR B-1 은 2013~2014 년동안생산력검정시험에서초형이우수하고복합내병성이면서수량성이양호하여 상주 51 호 로계통명을부여하였다. 상주 51 호 는 2014~2016 년 3 년간지역적응시험을수행한결과, 복합내병성을갖추고밥맛이양호한계통으로농촌진흥청농작물직무육성신품종선정심의회를거쳐 성산 으로명명되었다. 주요특성을요약하면다음과같다. 중산간지보통기보비재배에서 성산 의평균출수기는 7 월 27 일로오대벼보다 3 일늦고, 등숙비율은 88.2% 로오대벼보다높으며현미천립중은 20.2g 으로중소립종이다. 성산 은도열병, 줄무늬잎마름병, 흰잎마름병 (K1~K3) 에강하지만, 기타바이러스병및해충에는약하다. 성산 의쌀알은맑고투명하며백미완전립률및완전미도정수율이각각 92.0 및 68.3% 로오대벼보다높다. 중산간지에서보통기보비재배에서 성산 의쌀수량은 5.52MT/ha 로오대벼보다 5% 증수하였다. 성산 의적응지역은남부중산간지, 북부평야지및중산간지, 남부고냉지, 동해안북부해안지이다. 사사 : 본연구는농촌진흥청아젠다사업 ( 과제번호 : PJ ) 의지원에의해수행되었음 * 주저자 : Tel , jjkim74@korea.kr PA-32 Hyun-Su Park *, Man-Kee Baek, Jeong-Kwon Nam, Woon-Chul Shin, Choon-Song Kim, Gun-Mi Lee, Seul-Gi Park, Young-Chan Cho, Bo-Kyeong Kim We developed the Breeding Materials with Diverse Grain Size and Shape in japonica rice (BM_DGS). To characterize BM_DGS, we analyzed allele types for three grain size-related genes, GW2, GS3, and SW5. The alleles of GW2, GS3, and SW5 showed two types, wild type or loss-of-function mutant type. Deuraechan and Boramchan had wild types of GW2 and GS3 and mutant type of SW5, GW2GS3sw5. Daeripbyeo 1, Korean large grain cultivar, had wild type of GS3 and mutant types of GW2 and SW5, gw2gs3sw5. Jizi1581, extremely large grain japonica germplasm, had wild type of SW5 and mutant types of GW2 and GS3, gw2gs3sw5, and the other extremely large grain germplasm, Jizi1560, had mutant types of all three genes, gw2gs3sw5. In BM_DGS, eight allele combinations, all possible combinations using three genes, were confirmed. We evaluated the effect of genes on phenotypic variation of grain-related and agronomic traits. The genes mostly affecting grain length (GL), grain width (GW), grain thickness (GT), and 1,000-grain weight (TGW) were GS3, GW2, GW2, and GW2, respectively. The loss-of-function mutant allele type, gw2, gs3, and sw5, had an effect on increasing grain size. Among allele combinations, gw2gs3gw5 exhibited the largest and heaviest grain while GW2GS3SW5 was the smallest and lightest grain. GW2gs3SW5 resulted in the highest ratio of length to width (RLW) by long and slender grain. The BM_DGS carrying gw2 showed lower panicle number (PN), ratio of ripened grain (RRG), and yield than those of wild type. The BM_DGS carrying gs3 showed earlier heading and lower RRG and yield. The BM_DGS carrying sw5 exhibited much PN but lower number of spikelets per panicle (NS) while had no effect on RRG and yield. The results of this study could be useful to elucidate the relationship between grain size-related genes and grain-related and agronomic traits of BM_DGS. Also, the BM_DGS are being utilized in the breeding programs to diversify the grain size and shape of japonica rice. *Corresponding Author: Tel , mayoe@korea.kr 62

105 PA-33 강범규 1*, 김현태 1, 서정현 1, 신상욱 1, 김홍식 1, 윤홍태 2, 이영훈 3, 오은영 1, 정찬식 3, 오인석 2, 곽도연 제주지역은국내나물용콩생산량의 80% 이상을차지하는나물콩주산지이다. 이지역은소립콩을생산하기에는적합하지만토성과지리적위치및기후에의해경장과꼬투리가달리는높이 ( 협고 ) 가낮아져기계화수확에적합하지않다. 이에경장과협고가높으면서도도복에강하고, 콩나물재배특성이우수한나물용콩품종 아람 을개발하였다. 아람 은 보석 을모본으로 Camp 를부본으로 2007 년인공교배하여 년 F 1 및 F 2 세대를집단으로전개하고개체선발하였다. 10 년에는아시아채소연구개발센터 (AVRDC) 에서 F 3 F 4 세대촉진을거쳐 11 년 F 5 세대에서계통선발하고 년생산력검정시험을수행하였다. 경장이크고소립이며도복에강한특성으로 밀양 283 호 의계통명을부여하고 년 3 년간제주등 4 지역에서지역적응시험을수행하였다. 아람 은유한신육형이며화색은자색, 모용색은회색, 엽형은삼각형의특성을지니고있다. 종실은황색종피, 담갈색제색, 구형이며백립중은 9.9g 으로 풍산나물콩 대비 1g 가볍다. 생육특성은성숙기가 10 월 15 일로 풍산나물콩 대비 7 일늦은만숙종이며, 경장은 65cm, 협고는 13cm 로 풍산나물콩 대비각각 11cm, 2cm 높다. 아람 은불마름병과 SMV 모두강하였고, 내도복성은 2 배밀식재배조건에서 풍산나물콩 대비강하였다. 아람 의발아율은 96% 로 풍산나물콩 대비 2% 높았고, 콩나물수율은 598% 로 풍산나물콩 과비슷하였다. 수량성은적응지역평균 359kg/10a 로 풍산나물콩 대비 11% 증수되고, 특히제주 (318kg/10a) 에서는 20% 증수되었다. * 주저자 : Tel , hellobk01@korea.kr PA-34 강범규 1*, 김현태 1, 서정현 1, 신상욱 1, 김홍식 1, 최만수 2, 윤홍태 3, 고종민 2, 백인열 1, 한원영 1, 이영훈 2, 이병원 3, 오기원 1, 김현영 2, 곽도연 콩은동계작물과의이모작작부체계를구성하기위해서는생육기간이 일정도로짧아야한다. 그러나짧은생육기간으로수량이감소하고, 그동안개발된조숙성품종들은탈립성이높아적기에수확하지못할경우손실량이많았다. 장올 은표준품종인 새올콩 보다경장이크고마디수가많아수량이높고탈립성이개선된품종이다. 장올은 SS86001 을모본으로 수원 192 호 를부본으로 2001 년인공교배하여조합번호 SS01402 를부여하고 F 1 F 2 세대를집단으로전개후선발하였다. F 3 세대부터계통육종법으로선발하고 년생산력검정시험을수행하였으며 밀양 235 호 의계통명을부여한후 년 3 년간 5 개지역에서지역적응시험을실시하였다. 장올 은유한신육형이며엽형이난형이고화색은백색, 모용색은회색인질적특성을가지고있다. 지역적응시험수행결과경장이 64cm, 마디수가 14 개로 새올콩 대비각각 19cm 길고, 3 개많았다. 종실은구형이며제색과종피는황색이나백립중이 18.9g 으로중립종의특성을지니고있다. 생육일수는지역에따라 일이며 새올콩 과유사하였다. 불마름병과콩모자이크바이러스 (SMV) 는 새올콩 보다강하였다. 성숙협을 시간처리후협개열성을조사한결과개열협비율이 0% 로서탈립에강하였다. 수량성은전국평균 212kg/10a 로 새올콩 대비 9% 증수되었다. * 주저자 : Tel , hellobk01@korea.kr 63

106 PA-35 최유현 1, 김형태 1, 임기병 1,2* 1 2 울릉도는 Neogene 에서 Quaternary 에있었던화산활동으로만들어진화산섬으로풍화와침식작용으로인해해안의해식애, 깊은계곡등울릉도만이지니는독특한지형이형성되었다. 이후도입식물및섬내부에서종분화과정으로생성된자생종을포함하여약 550 여종이현재서식하는것으로알려져있다. L. hansonii ( 섬말나리 ) 는 section Martagon 속하는울릉도자생종으로서 3 열의윤생하는잎과크고노란꽃으로다른 lily 들과비교해독특한특징을가지고있어상품성이높을것으로여겨지나현재까지연구는주로계통학적연구에제한되어왔다. 따라서향후 L. hansonii 를상업적으로이용하기위하여다양한연구가필요하다. 울릉도나리령과나리분지에서채집한 L. hansonii 를재료로하여집단간및집단내의유전학적변이를관찰하고자 Fluorescent In situ Hybridization (FISH) 방법을이용, 세포내염색체의 45S ribosomal DNA 와 5S ribosomal DNA 의개수, 위치를확인하였다. 이전연구에서는 section Martagon 에속하는 L. distichum ( 말나리 ) 과 L. tsingtauense ( 하늘말나리 ), L. martagon ( 서양말나리 ) 은염색체내 45S 와 5S rdna 의개수가각각 8 개, 2 개로개체간동일하게나타난다고확인되었다. 그러나 L. hansonii 의경우나리령에서집단내 45S rdna 가 개로다양하게나타났으며 5S rdna 는 2 개씩동일하게나타났다. 나리분지에서는 45S rdna 가 14 개, 16 개로관찰되었으며 5S rdna 는 2 개로나리령과동일하게관찰되었다. 결과적으로 section Martagon 에속하는 Lillium 들의 5S rdna 는 2 개로변함이없으며오직 L. hansonii 에서만 45S rdna 개수의차이를확인할수있었다. 본연구결과는섬에서식하는자생종이본토에서식하는근연종보다유전적다양성이작다는일반적인결과와상반된다. 이는섬말나리종형성시기혹은이후섬말나리가울릉도전역에군집을확장하는과정에서유전적으로차이가있으나형태적으로유사하며생식적격리가없는집단의이주 (migration) 가있었을것으로여겨진다. 사사 : 이논문은 2016 년도정부 ( 교육부 ) 의재원으로한국연구재단의지원을받아수행된기초연구사업임 ( 과제번호 : NRF-2016R1D1A1B ) * 교신저자 : kblim@knu.ac.kr PA-36 Hye Jeong Kim 1, Hyun Suk Cho 1, Jae Seong Kim 1, Jin Sol Park 1, Wan Woo Yeom 1, Jae Yong Han 1, Jeong-Il Kim 2, Young Soo Chung 1* 1 2 Plants use light as a source of both energy and information about their environment. Among photoreceptors, cryptochromes are blue-light receptors that regulate multiple light responses including hypocotyl growth, flowering, circadian clock and gene expression in plants and animals. Transgenic soybean plants overexpressing AtKDA1 were produced by Agrobacterium-mediated transformation using the modified half-seed method. The integration of the transgene was confirmed from the genomic DNA of transformed soybean plants using PCR, and the copy number was determined by Southern blotting using leaf samples from T 2 seedlings. To examine the effect of AtKDA1 expression on plant height and its yield in transgenic plants, we compared their growth characteristics to those of non-transgenic (NT) plants. The chosen three transgenic seedlings (#9, #10 and #15) exhibited longer hypocotyls than NT seedlings. Agronomic traits including plant height, the number of nodes per plant, branches per plant, pods per plant and total seed weight were also investigated in GMO field. In phenotypic analysis, AtKDA1 transgenic plants showed longer plant heights than NT plants. *Corresponding Author: Tel , chungys@dau.ac.kr 64

107 PA-37 Jin Sol Park 1, Hye Jeong Kim 1, Hyun Suk Cho 1, Jae Seong Kim 1, Wan Woo Yeom 1, Jae Yong Han 1, Dae-Jin Yun 2, Young Soo Chung 1* 1 2 YUCCA6 gene was introduced to produce drought tolerant transgenic soybean plants via Agrobacterium-mediated transformation using the improved half-seed method. The presence of the gene in transgenic plants were confirmed by PCR and Southern blot analysis, and the expression was investigated by RT-PCR. Transgenic line #2, #3 and #5 were tolerant to drought stress while non-transgenic plants were withered completely. Line #2, #3 and #5 were not affected remarkably by water deficit condition and lead to enhanced drought tolerance due to the prevention of cell membrane damage and maintenance of chlorophyll content. Moreover, the enhanced drought tolerance in transgenic lines resulted in reduced transpiration rate and low ROS content. *Corresponding Author: Tel , chungys@dau.ac.kr PA-38 Dong-Min Kim 1*, Eun-Jo Shim 1, Seung-Eun Park 1, Shin-Sook Park 1, Gyu-Chan Shim 2, Seung-In Yi Rice seed storability is an important characteristic of seed quality. Presently, little is known about the genetic and physiological mechanisms controlling rice seed storability. In this study, a molecular linkage map consisting of 133 SSR markers was employed to identify QTL associated with seed storability. 96 introgression lines derived from a cross between Hwaseung as the recurrent parent and Oryza rufigopon as a donor parent and parents were evaluated for traits including seed vigour(sv) and germination percentage(gp) under the artificial aging. A total of nine QTLs were detected on chromosome 3, 4, 6, 9 and 10 and the phenotypic variance explained by each QTL ranged from 14.0 to 58.9%. The O. rufipogon allele contributed the positive effect at these nine QTL loci. These results provide important information for understanding the genetic mechanisms on rice seed storability, and will be useful for breeding new rice varieties with high seed storability. *Corresponding Author: Tel , acekdm@korea.kr 65

108 PA-39 Jun Soo Kwak 1*, Won Joo Shin 1, Beom Seok Choi 1, Hak Soo Seo 1 1 Bacterial leaf blight is one of the major diseases in rice and affects yields. Thus, various methods have been applied to protect rice from this disease. Here, we show systemic translocation of the human drug niclosamide (5-chloro--(2-chloro-4-nitrophenyl)-2-hydroxybenzamide)in rice and its long-term effect on prevention of rice leaf blight. The development of pv-induced rice leaf blight was effectively inhibited in untreated systemic leaves as in niclosamide-treated leaves, although its effect gradually decreased in a time-dependent manner. Time-course examination after niclosamide treatment showed that the niclosamide level was highest after 3 h in non-treated distal leaves, suggesting fast systemic movement of niclosamide from the treated local site to untreated distal regions. Our data indicate that niclosamide controls rice leaf blight by its rapid systemic movement and that its effect is maintained for a long time. Acknowledgement: This work was supported by a grant from the Next-Generation BioGreen 21 Program (Plant Molecular Breeding Center no. PJ ), Rural Development Administration, Republic of Korea. *Corresponding Author: Tel , seohs@snu.ac.kr PA-40 Sang Woo Park 1*, Woo Sub Kim 1, Hak Soo Seo 1 1 Seed germination is an important stage in the lifecycle of a plant because it determines subsequent vegetative growth and reproduction. Here, we show that the E3 SUMO ligase AtSIZ1 regulates seed dormancy and germination. The germination rates of the siz1 mutants were less than 50%, even after a short period of ripening. However, their germination rates increased to wild-type levels after cold stratification or long periods of ripening. In addition, exogenous gibberellin (GA) application improved the germination rates of the siz1 mutants to the wild-type level. In transgenic plants, suppression of AtSIZ1 caused rapid post-translational decay of SLEEPY1 (SLY1), a positive regulator of GA signaling, during germination, and inducible AtSIZ1 overexpression led to increased SLY1 levels. In addition, overexpressing wild-type SLY1 in transgenic sly1 mutants increased their germination ratios to wild-type levels, whereas the germination ratio of transgenic sly1 mutants overexpressing msly1 was similar to that of sly1. The germination ratios of siz1 mutant seeds in immature developing siliques were much lower than those of the wild type. Moreover, SLY1 and DELAY OF GERMINATION 1 (DOG1) transcript levels were reduced in the siz1 mutants, while the transcript levels of DELLA and ABSCISIC ACID INSENSITIVE 3 (ABI3) were higher than those of the wild type. Taken together, these results indicate that the reduced germination of the siz1 mutants results from impaired GA signaling due to low SLY1 levels and activity, as well as hyperdormancy due to high levels of expression of dormancy-related genes including DOG1. Acknowledgement: This work was supported by a grant from the Next-Generation BioGreen 21 Program (Plant Molecular Breeding Center no. PJ ), Rural Development Administration, Republic of Korea. *Corresponding Author: Tel , seohs@snu.ac.kr 66

109 PA-41 Joo Yong Kim 1*, Kyu Ho Lee 1, Hak Soo Seo 1 1 Ubiquitination and sumoylation are essential post-translational modifications that regulate growth and development processes in plants, including control of hormone signaling mechanisms and responses to stress. This study showed that COP1 (Constitutive photomorphogenic 1) regulated the activity of Arabidopsis E3 SUMO (Small ubiquitin-related modifier) ligase AtSIZ1 through its E3 ubiquitin ligase activity. Yeast two hybrid analysis demonstrated that COP1 and AtSIZ1 directly interacted with one another, and subcellular localization assays indicated that COP1 and AtSIZ1 co-localized in nuclear bodies. Analysis of ubiquitination showed that AtSIZ1 was polyubiquitinated by COP1. The AtSIZ1 level was higher in cop1-4 mutants than in wild-type seedlings under light or dark conditions, and overexpression of a dominant-negative (DN)-COP1 mutant led to a substantial increase in AtSIZ1 accumulation. In addition, under drought, cold, and high salt conditions, SUMO-conjugate levels were elevated in DN-COP1-overexpressing plants and cop1-4 mutant plants compared to wild-type plants. Taken together, our results indicate that COP1 controls responses to abiotic stress by modulation of AtSIZ1 levels and activity. Acknowledgement: This work was supported by a grant from the Next-Generation BioGreen 21 Program (Plant Molecular Breeding Center no. PJ ), Rural Development Administration, Republic of Korea. *Corresponding Author: Tel , seohs@snu.ac.kr PA-42 Min Jeong Hong 1, Dae Yeon Kim 2, Joon-Woo Ahn 1, Sang Hoon Kim 1, Hong-Il Choi 1, Si-Yong Kang 1, Yong Weon Seo 2, Jin-Beak Kim 1* 1 2 F-box protein is components of the SCF (Skp1-Cullin-F-box protein) E3 ubiquitin ligase complex which binds specific proteins destinated for ubiquitination and degradation. F-box proteins are known to play a critical role in the regulation of various development processes in plants. However, detailed functions of F-box proteins in wheat remain unknown. In order to collect F-box gene family through genome-wide survey, the draft genome sequence of wheat (IWGSC Reference Sequence v1.0 assembly) was used. About 950 F-box protein genes have been identified in the wheat genome. We used Illumina Hiseq 2000 sequencing platform for expression analysis of F-box protein genes according to expression level at wheat developmental stages. We obtained the full length sequences of 3 F-box protein genes (TaFBX1: Traes_2AS_3ADE97AEF, TaFBX2: Traes_7AL_001F061F8, and TaFBX3: Traes_1BL_80A1C84CE), which contains a putative F-box domain and leucine-rich repeats (LRR). The TaFBX genes were differentially expressed in wheat tissues and respond to grain developmental stages. To determine the subcellular localization of wheat F-box proteins, we examined the sub-cellular localization of TaFBX-GFP fusion proteins in a transient assay using tobacco (Nicotiana benthamiana). Our data demonstrated that GFP-tagged TaFBXs were targeted to the plasma membrane and nucleus. Using the yeast two-hybrid screen, we identified TaFBXs-interacting proteins in a wheat library. And also, we performed yeast two-hybrid assay to investigate protein-protein interaction between TaFBX proteins and various wheat SKP-like proteins. Our study of putative F-box proteins will aid on understanding the functions of F-box proteins in wheat. *Corresponding Author: Tel , jbkim74@kaeri.re.kr 67

110 PA-43 박종택 *, 정재아, 박상근, 김미선 거베라 Pinkie Girl' 은 2008 년수원국립원예특작과학원에서분홍색반겹꽃 Free Zone 과살구색 Atilia 를인공교배하여실생계통을양성하고, 실생계통으로부터선발한연분홍색 09B3-76 을부본으로사용하고, 진분홍색 Lila 를모본으로 2011 년인공교배하였다. 인공교배로획득한종자로부터실생계통을양성하여, 화색이선명하고화폭이큰분홍색의스탠다드반겹꽃 원교 12B3-58 을개체선발하였다. 선발된개체에대하여 2012 년조직배양으로개체를증식한후 2012 년부터 2014 년까지 1 2 차생육특성검정을수행하고, 2015 년에 원교 B3-58 호 로계통명을부여하여 3 차특성검정, 안정성 균일성에대한연차별재현성및기호도평가를수행한결과, 화색및화형에대한기호도가우수하고, 연간채화량이많은다수성품종으로그우수성이인정되어, 2015 년농촌진흥청직무육성품종심의회를거쳐 Pinkie Girl 로명명되고직무육성품종으로등록되었다. Pinkie Girl 의생육및개화특성은화색과화형이유사한 Chavez 를대조품종으로하여조사하였다. Pinkie Gril 의설상화는 RHS color chart RP65C 의분홍색반겹꽃으로, 대조품종의 R38D 보다선명한분홍색으로갈색의화반과어우러진다. Pinkie Girl 의화경과내화경이각각 12.0±0.2cm, 5.7±0.3cm 으로대조품종의 11.3±0.2cm, 5.4±0.3cm 보다큰대륜계품종이며, 내부설상화가장자리가규칙적이다. 화반직경은 3.0±0.2cm 로대조품종의 4.0±0.1cm 보다작다. 외부설상화의길이와폭은 5.4±0.3cm, 1.2±0.1cm 로넓은도란형이다. 잎의길이와폭은 46.2±2cm 와 15.4±1cm 이며결각은보통수준이다. 절화장은 68.8±4.1cm 로길며, 절화수명은평균 12.9±1.5 일로대조품종 9.0±0.3 일보다길었다. 또한연간채화량이 54.0±3.5( 본 / 주 ) 로다수성품종이다. * 교신저자 : Tel jongtaek@korea.kr PA-44 Suk-Man Kim 1,2, Kyeong-Ho Kang 3, Jong-Min Jeong 3, Russell Reinke Direct seeding of rice is increasingly being adopted by rice farmers to establish their crops as a means of saving labor and reducing costs. However, the method often results in a poor environment for germination as excessive water levels after seeding can cause poor seedling establishment and a concomitant reduction in yield potential, especially in submergence prone areas. In this study we discovered QTLs associated with tolerance of anaerobic germination in new genetic accessions using genotypic data derived from the Illumina 6K SNP chip. The mapping population developed for QTL analysis comprised a total of 285 F 2 plants derived from across between an AG tolerant indica variety Tai Nguyen (TN) and a susceptible variety Anda. Using 872 SNPs showing polymorphism between the parents, a genetic linkage map was constructed with a mean of 67 markers harbored within each chromosome with a total length of 1,448.45cM and an average interval length of 1.7cM. A total of three QTLs associated with AG tolerance were identified in the population, qag1a and qag1b on chromosome 1 and qag8 on chromosome8 using composite interval mapping (CIM). The percent of variance explained by these QTLs ranged from 5.49 to %. The lines with three QTLs (qag1b+qag1a+qag8) demonstrated approximately 50% survival rate under anaerobic conditions Corresponding Author: Tel , s.kim@irri.org 68

111 PA-45 Tien Dung Nguyen, Sung Aeong Oh, Soon Ki Park Plants are frequently exposed to environment including adverse changes such as temperature stresses. Global climate changes have been being a big challenge for crops, of which, high temperature stresses are known to be more serious in plants occasionally leading male sterility at many the disruption of pollen development leading male sterility. In this study, we demonstrate that a high temperature stress caused completely male sterility due to inhibition of pollen development from microspore stage. High temperature affected plants to decrease length of stamens containing no viable pollen, but not on female organs, indicating that male reproductive organs were more sensitive to heat stress than female. Interestingly we observed that sizes of heat-induced pollen were enlarged to similar level with those from 4X plants. In addition, using marker lines, we found that two set of 8 chromosomes were produced in heat-induced pollen grains. These results strongly indicate that high temperature stress not only causes male sterility, also could induce polyploidy pollen in Arabidopsis. Corresponding Author: , psk@knu.ac.kr PA-46 Jagadeesh Sundaramoorthy 1, Gyu Tae Park 1, Seokhyung Lee 1, Jeong-Dong Lee 1, Hak Soo Seo 2, Jong Tae Song 1* 1 2 Saponins are a group of secondary metabolites available in soybean [Glycine max (L.) Merr.]. Soybean saponins are classified as group A and DDMP saponins. Group A saponins are undesirable component of food products due to its bitterness and astringency. In contrast, DDMP saponins and their derivatives are not possessing bitterness and astringency and beneficial to human health when consumed as regular diet. We recently isolated two EMS mutant lines (PE2248 and PE2371) with DDMP-deficient saponins. The objective of the present study is to identify and characterize the gene which is encoding a protein responsible for biosynthesis of DDMP saponins, and then finally to reveal a role of saponins in soybean plants. Segregation analysis showed that the both the mutant phenotype is controlled by single recessive gene. The position of locus (Sg-9) involved in the biosynthesis of DDMP deficient saponins was mapped using bulk segregation analysis and fine-mapped on chromosome 16 (130 kb) between two SNP markers. The genomic sequencing of Sg-9 gene showed a single nucleotide polymorphism in PE2248 (G626A) and PE2371 (C137T) mutant lines. Complementation between two mutations by crossing of PE2248 and PE2371 exhibited that the mutation sites lie on the same gene. Further, Sg-9 gene characterization will be carried out by cloning and overexpression studies. Acknowledgement: This work was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) fund by Ministry of Education (No. NRF-2016R1D1A1B ). *Corresponding Author: Tel , jtsong68@knu.ac.kr 69

112 PA-47 Rupesh Tayade 1*, Tien Dung Nguyen, Lee Dasom 1, Sung Aeong Oh 1, Ki Hong Jung 2, Soon Ki Park * 1 2 Abiotic stress such as heat and drought are predominating in the current environment scenario. In recent decades global warming is major concern as increasing environment temperature is an alarming threat to crop production worldwide. Recently several genes that confer tolerance to environmental stress have been reported. However, the intensities of tolerance vary significantly depending on cultivars, growing conditions, and mechanisms. In this study, we investigated the profiles of abiotic stress tolerant genes, six-candidate genes of NDPK2, AtGolS1, PRE1, YUCCA6, AtHSP101 and CBF1 from Arabidopsis Thaliana and constructed twenty four vectors combined with four promoters driving constitutive and pollen/anther specific expression, UBQ14, TDF1, RMP1 and OsLPS1. We conducted Agrobacterium-mediated transformation to generate transgenic Arabidopsis plants and rice as heterologous system. In T 2 generation, Arabidopsis transgenic plants will be evaluated further for heat stress at seedling, flowering and other developmental stages traits whereas rice T 0 plants will be evaluated in the field condition for heat stress tolerance. The comparative evaluation may give the border understanding of selected genes and find out more efficient gene for heat stress tolerance and its applicability to other crops. *Corresponding Author: Tel , psk@knu.ac.kr PA-48 Saima Akhter, Ha Thi Lieu, Rupesh Tayade, Sung Aeong Oh, Soon Ki Park Pollen development can be considered as model system not only for the analysis of important processes in plant reproduction but also for learning diverse biological aspects. Gametophytic cytokinesis is necessary for the development and function of male and female gametophytes. In this study we report here that a gametophytic mutant line isolated in activation-tagging pool. Pollen developmental analysis revealed that the asymmetric mitotic division in microspore is compromised by the mutation, leads to the repositioning of the cell plate and partial or complete failure of cytokinesis. Transmission analysis showed that defects in both gametophytes, but severe defects observed in male gametophytes and leads to the production of divided or twin celled pollen. Map-based cloning was used to identify the gene causing mutant pollen phenotype. Mapping population was generated through reciprocal crossing of mutant plant with different ecotype plant and genetic analysis of F 2 plants were carried out. To define chromosomal region causing the mutation we conducted PCR-based analysis of approximately 800 F 2 plants using SSLP markers and narrowed down the region up to 445Kb in chromosome 1. In conclusion the region we narrowed down can serve as candidate for further fine mapping of responsible gene. Corresponding Author: Tel , psk@knu.ac.kr 70

113 PA-49 김진희 *, 이은수, 안율균, 김도선, 홍종필, Abinaya Manivannan, 최승국 SNP(single nucleotide polymorphisms) 는마커의빈도수가풍부하여개발이비교적용이하고비용이저렴하며, 무엇보다최근에다양한 high-throughput 분석기술로 SNP 분석의자동화가가능한장점이있어, 육종조합에적용이가능한마커세트를확립할수있다. 무는배추와함께우리나라 2 대채소로 1 인당소비량은 19.6kg(2012 년기준 ) 포함되는등경제적으로주요한작물이며김치의부재료로사용되는등그이용범위가매우넓다. 국내무가세계적인경쟁력을갖추기위해서는분자표지를이용한분자육종시스템구축이요구된다. 이러한무의분자표지기반육종과정을효율적으로하기위해본연구에서는육종에서주로활용되는형질들을파악하여대표육성계통 35 개를선정한후 Illumina Hiseq 4000 을통해전사체염기서열분석을진행했다. 계통선정에는추대성, 비대양상, 근장, 엽형, 근색, 육색, 형태등의형질을고려했고, 조합이달라져도여교배등의육종과정에적용될수있도록선정하였다. 계통별평균염기서열은 5,869,258,936 bp(58,509,167 reads) 이며, GC 함량은 48%, Q30 값은 97% 이다. 전사체염기서열의비교를통하여다양한무계통들의 SNP 를분석했으며다수의 SNP 를확보했다. 선발한 327 개의 SNP 는품종분류, 분자마커를이용한여교배, 형질마커개발, 유전지도작성등다양한목적에따라 Fluidigm 을이용한 SNP 분자마커세트로개발할예정이며가까운미래에이러한 SNP 마커풀을이용해다양한육종프로그램에대응할수있을것으로기대한다. * 교신저자 : Tel , sayzinni@korea.kr PA-50 Joung-Ho Lee 1, Jeong-Tak An 1, Koeun Han 1, Seula Choi 1, Muhammad Irfan Siddique 1 and Byoung-Cheorl Kang 1* 1 Chili veinal mottle virus (ChiVMV) is the notorious virus affecting the severe loss of pepper production in Asia and Africa. To map the positions of ChiVMV resistance genes, F 2 mapping populations were constructed by crossing each resistance accession, Capsicum annuum, CV3, CV4, and CV8 with susceptible C. annuum, Jeju. In the CV3 and CV8 mapping populations, resistance genes were inherited by a single dominant manner and located on the short arm of pepper chromosome 6. The ChiVMV resistance marker reported in the previous study was co-segregated with these genes. Through allelism test of resistance genes from CV3 and CV8, it was revealed that the resistance gene in each line was originated from the same locus, and we named this gene, Cvr1 (ChiVMV resistance 1) locus. We developed several SNP markers linked to Cvr1 using pepper genome information and pepper bacterial artificial chromosome (BAC) sequences. By contrast, the inheritance mode of ChiVMV resistance in CV4 was different from those of CV3 or CV8. The inheritance study showed that two independent complementary genes involved in ChiVMV resistance in CV4. To map the resistance genes in CV4, SNP-based linkage map was constructed by genotyping-by-sequencing (GBS) method. The result of this study will accelerate the ChiVMV resistance research and breeding resistance cultivar to ChiVMV in pepper. *Corresponding Author: Tel. 02) , bk54@snu.ac.kr 71

114 PA-51 Muhammad Irfan Siddique 1, Koeun Han 1, and Byoung-Cheorl Kang 1* 1 Plant breeding needs genetic variation of economical traits to develop new, high-yielding and improved cultivars. EMS-induces mutation has been practiced to cause mutations at loci regulating economically important traits and/or to knock out the genes to elucidate their functions. The present study was under taken to induce mutations in a Capsicum annuum Micro-Pep. Micro-Pep is a small, pungent pepper generally used as ornamental purpose. The accession is convenient to use in mutation study and molecular research due to its compact growth habit, and small size. The seeds were treated with 1.3% of mutagen to induce mutation and 30% less germination percentage was observed in EMS treated seeds in comparison to control seeds. A total of 6,620 M 1 plants were grown under greenhouse condition and 3996 M 2 mutant lines were harvested. Among these mutants 700 lines were evaluated for novel traits. The mutants were observed with various phenotypic variants such as plant growth, habit, leaves color and shapes, flower and fruits morphology. These Micro-pep mutant collections will serve as platform for TILLING by sequencing to accelerate functional genomics research in pepper. In parallel to this, mutants were screened for non-pungent mutants using Gibbs reagent method. In a result, 1 putative non-pungent mutant was identified. These mutant phenotypes and non-pungent mutant s will be valuable genetic resources for identification of functional genes and molecular breeding of pepper. *Corresponding Author: Tel , bk54@snu.ac.kr PA-52 Su Jang, Hyekyung Son, Taejun Kim, Jinwoo Lee and Hee-Jong Koh * Degree of leaf rolling is one of the important traits that determines plant architecture. Moderate leaf rolling can contribute to improve photosynthetic efficiency not only at individual level but also at population level by increasing erectness of upper leaf. Furthermore, this morphological trait is closely related to planting density, ventilation, and drought resistance. In this study, RILs from cross between Tong-il type variety M23 and japonica variety T887 were used to identify QTL controlling leaf rolling. this population was phenotyped for rolling index of flag leaf (FLRI) and upper second leaf (SLRI) for two years. Total six QTLs for FLRI and SLRI on chromosome 1, 2, and 9 were identified using composite interval mapping. One stable QTL located on chromosome 9 showed consistent effects for FLRI and SLRI in both years, explaining % and % of phenotypic variation. Two QTL on chromosome 2 that were detected in almost the same region were also associated with both traits, implying that two leaf rolling traits could be controlled by same genetic mechanism. The result of QTL analysis would contribute to understanding genetic basis for leaf rolling and these QTLs identified in this study can be used in rice breeding program to improve plant architecture. *Corresponding Author: Tel , heejkoh@snu.ac.kr 72

115 PA-53 Zhuo Jin, Jin seok Moon, Somyung Lee and Hee-Jong Koh * Rice (Oryza sativa L.) is one of the most important food crops. There have been tremendous efforts to increase rice productivity via genetic improvement based on understanding of developmental mechanisms associated with grain yield. To date many lesion mimic mutants have been identified in rice and these mutants are important to understand the mechanism of the programed cell death and disease resistance. In this study we identified a new light-dependent lesion-mimic mutant in rice, through a chemical mutagenesis on Koshihikari with Ethyl methanesulfonate (EMS). The mutant displayed a lesion-mimic phenotype in the absence of pathogen attack and also early senescence phenotype. Lesions appeared on the leaves of the mutant at early stage, and at flowering stage lesions were dispersed over the entire leaf surface. After flowering the mutant showed a significant decrease in chlorophyll content, and high concentrations of reactive oxygen species (ROS). Genetic analysis revealed that spot leaf was caused by incomplete dominant mutation. Using bulked segregant analysis method and sequence-tagged site (STS) markers, the gene that cause lesion-mimic and early senescence phenotype, was located at chromosome 5 within a physical region of 2Mb. The MutMap method is being applied for cloning the gene by a whole-genome resequencing of pooled DNAs from a segregating population. *Corresponding Author: Tel , heejkoh@snu.ac.kr PA-54 Hyoung Tae Kim * and Je Min Lee Three complete mitochondrial genomes (chondrome) (two in Solanum lycopersicum and one in S. pennellii) were newly assembled and the intra- and interspecific variations of chondrome were analyzed. The lengths of three chondrome sequences were 423,596 ~ 446,257 bp. Although there were numbers of rearrangement between chondromes of S. lycopersicum and S. pennellii, over 97% of chondrome sequences were very similar with each other. In terms of intracellular gene transfer (IGT) between organelle genomes, 9,598 bp (2,558 bp in large single copy, 32 bp in small single copy, and 7,008 bp in inverted repeat) of plastome sequences were found in three chondromes in common. Among mitochondrial plastid DNAs (MTPTs) in tomato, five MTPTs were exclusively detected in chondrome of S. pennellii. These five MTPTs seems to be deleted in the chondrome of S. lycopersicum because three and five of them were found in chondromes of Nicotiana and Capsicum, respectively. In addition, Numbers of nuclear copies of mitochondrial DNAs (NUMTs) and nuclear copies of plastid DNAs (NUPTs) were found in nuclear genomes of S. lycopersicum and S. pennellii. Hotspots of NUMTs and NUPTs were found as well. Among IGTs between nuclear genome and chondrome, three of them were recently occurred from nuclear genome to chondrome in S. pennellii but most of them are likely to be transferred from organelle genomes to nuclear genome. As a result, our results support that endosymbiotic gene transfers have frequently occurred recently in tomato genomes. *Corresponding Author: rladbgus@gmail.com 73

116 PA-55 최재근 1*, 박종열 1, 장은하 2, 류시환 1, 서영호 1, 박기진 1, 남궁민 1, 윤석원 1, 용우식 1, 최준근 옥수수연구소에서팝콘용튀김옥수수연구를 1997 년부터시작하여현재오륜팝콘, 지팝콘을개발하여국내재배농가에보급하고있다. 현재국산품종을이용한팝콘은국내산을선호하는소비자층을중심으로소비가되며원료공급을위한친환경재배단지가조성되어있다. 소비자가손쉽게접할수있게팝콘으로튀긴가공상품으로개발되어완제품으로공급되고있으며또한농촌체험활동및가정에서간식대용으로즉석에서튀겨먹는용으로알곡으로만포장되어소비자에게공급되고있다. 영화관에서주로소비되는국내팝콘시장은연간 3 천억이상의규모로형성되어있으나대부분수입산알곡을이용하고있다. 앞으로국내에서개발한품종으로수입산을대체하기위하여 2012 년에강원도옥수수연구소에서 오륜 품종을개발하여전국에 20ha 의재배단지를형성하여국내시장에보급되고있다. 본연구는오륜품종보다수량성및튀김율이높은품종을개발하고자수행하였다. 오륜 2 호 은튀김용모집단에서분리된 GP5 를모본, GP3 를부본으로하여개발하였으며, 수량성은 오륜 대비 9% 증수한 497kg/10a 로수량이높으며, 도복지수는 2 로 오륜 의 3 보다낮아내재해성도강하며, 100 립중은 15.7g 으로 오륜 16.2g 보다소립인특성을갖고있다. 튀김율은알곡수분이 11.0~12.0% 일때 오륜 2 호 가 27 배로 오륜 의 25 배보다높아가공용으로도적합한특성을가지고있어앞으로농가에보급하여재배단지를확대하면수입산을대체할품종으로기대한다. * 주저자 : Tel , jaekeun@korea.kr PA-56 Hong-Il Choi 1, Sung Min Han 1, Sun-Goo Hwang 2, Cheol Seong Jang 2, Soon-Jae Kwon, Joon-Woo Ahn, Yeong Deuk Jo, Sang Hoon Kim, Si-Yong Kang 1 and Jin-Baek Kim 1* 1 2 Exposure to ionizing radiation has been regarded as a kind of abiotic stresses that can change the expression of genes in living organisms. This study aimed on investigating the variations in gene expressions induced by two different types of irradiations with different doses, which were low linear energy transfer (LET) gamma rays and high LET ion-beams on rice. RNA sequencing was carried out using the Illumina HiSeq-2500 platform. More than 90% of the RNA-seq reads were mapped to the rice reference genome sequence (IRGSP-1.0). A total of 247 differentially expressed genes (DEGs) were identified by comparison of the gene expression levels between the wildtype and the irradiated individuals. The 247 DEGs were divided into five modules and 27 intra-modular hub genes were found using the weighted correlation network analysis (WGCNA) method. The MEturquiose module had the most number of genes with 75 related to carbohydrate and small molecule metabolic processes. The co-expression network reconstructed using ARACNE (algorithm for reconstruction of accurate cellular networks) showed specific up- or down-regulation of the genes in each module according to the types and doses of radiation. This study will contribute to understanding the gene expression responses to ionizing irradiation. *Corresponding Author: Tel , jbkim74@kaeri.re.kr 74

117 PA-57 안억근 1*, 이상복 1, 이점호 2, 장재기 3, 원용재 1, 이정희 1, 정국현 1, 하운구 1, 정응기 1, 김명기 1, 정종민 1, 서정필 2, 양창인 년국내쌀생산량은 420 만톤, 일인당쌀소비량이 61.9 kg로생산량은감소하지않으면서소비는지속적으로줄어들고있어구조적으로쌀의공급과잉현상이심화되고있다. 사료용벼는논의형상과기능을유지하면서이러한수급불균형을해결할수있는좋은대안임에도불구하고현시점에서는밥쌀용벼와비교하여소득이낮아정부의보조금없이는농가현장에서재배를기피하는경향이있다. 이에수량성 ( 바이오매스 ) 향상등을통한경제적가치를올릴필요가있다. 이번에육성한사료용벼 청우 는도열병, 흰잎마름병 ( 균계 K1, K2, K3, K3a), 줄무늬잎마름병, 벼멸구및애멸구에저항성이있어병충해방제에들이는노력및비용을절감할수있어친환경안전조사료생산이가능할뿐만아니라농업경영비도절감할수있다. 또한 청우 의지상부건물수량은중부평야, 영남평야및호남평야지 4 개소에서 3 년간시험한결과평균 20.6 톤 /ha 으로 녹양 대비높은수량을나타내경제적가치도높일수있을것으로기대된다. 더구나가축이소화흡수할수있는사료의영양가지표인가소화양분총량 (Total Digestible Nutrients, TDN) 도일반사료작물과비교하여떨어지지않아사료적가치가높다고판단된다. 이처럼복합내병충성, 높은지상부건물수량및가소화양분을보유한 청우 는재배확대시쌀생산조정을통한수급조절에기여할것으로기대된다. 사사 : 본연구는농촌진흥청연구사업 ( 세부과제명 : 사료용벼유용형질도입계통육성, 세부과제번호 : PJ ) 의지원에의해이루어진것임. * 주저자 : Tel , okahn@korea.kr PA-58 안억근 *, 홍하철, 정국현, 원용재, 현웅조 사료용벼는논의형상과기능을유지하면서쌀수급불균형을해결할수있는좋은대안임에도불구하고현시점에서는밥쌀용벼와비교하여소득이낮아 ( 소득차이 : 177,090 원 /10a, KREI, 2011) 정부의보조금없이는농가현장에서재배를기피하는경향이있다. 이에이앙재배에비해생산비 10%, 노동력 22.8% 절감되는직파재배가필수적인데직파재배는잡초발생과관리가큰문제점으로되어있다. 이를해결하기위해화학돌연변이를통한이미다졸리논계제초제저항성사료용벼를선발하였다. 사료용벼중수량성과내병충성이좋은 영우 종자를 EMS(ethyl methane sulphonate) 처리하여돌연변이를유도하고자가수분으로 M 1 을양성한후 M 2 세대 6,000 개체를포장에이앙하여최고분얼기에 Imazaquin( 제품명 : 톤압 ) 을 400 ml ( 유효성분 20%)/10a 를처리하여정상적인생육을보이는저항성개체 4 개체를선발하였다. 현재돌연변이된부위탐색을목적으로 MutMap(a method based on whole-genome resequencing of pooled DNA from a segregating population) 방법적용을위해저항성개체에 영우 wild type 을여교배하여분석집단을창출하였다. 차후 whole-genome resequencing 후 reference sequence 와비교 (SNP index=1) 를통해변이부위를확인하고이미다졸리논계제초제저항성사료용벼개발및육종에이용할계획이다. 사사 : 본연구는농촌진흥청연구사업 ( 세부과제명 : 사료용벼유용형질도입계통생산력검정시험, 세부과제번호 : PJ ) 의지원에의해이루어진것임. * 주저자 : Tel , okahn@korea.kr 75

118 PA-59 Woo-Jong Hong $, Anil Kumar Nalini Chandran $, Jong-Seong Jeon * and Ki-Hong Jung * Signal transduction and transcriptional regulation pathways are key elements in the control of diverse physiological responses and agronomic traits in plants. The regulatory roles of more than 1,000 known genes have been functionally characterized in rice, a model crop plant, and many of them are associated with transcriptional regulation and signal transduction pathways. In this study, we collected and analyzed 417 known genes associated with regulatory pathways, about 40% of the known genes, using the regulation overview installed in the MapMan toolkit. Connecting novel genes to current knowledge about regulatory pathways can elucidate their molecular functions and inspire ideas for further applications. We have summarized the functions of known regulatory genes in the areas of transcriptional regulation, epigenetic regulation, protein modification, protein degradation, signaling and hormone metabolism, also we have emphasized the unique features of several gene families in these classes, including MADS box families, which are strongly associated with the regulation of floral organ identity and flowering time. In addition, our construction of functional modules in four agronomic categories, morphological, physiological, biotic stress and abiotic stress, suggests a basic framework for expanding current knowledge about regulatory pathways to enhance agronomic traits in rice. We also provide a quick illustration of the positive and negative regulatory relationships of the target gene to manipulate agronomic trait by using genome-wide transcriptome data of knockout or overexpression mutations of genes of interest in each functional module. *Corresponding Author: khjung2010@khu.ac.kr (K.-H. Jung), jjeon@khu.ac.kr (J.-S. Jeon) $ These authors contributed equally. PA-60 Hyangmi Park 1*, Yongjae Won 1, Kukhyun Jung 1, Hyun Ungjo 1, Hacheol Hong 1, Yulho Kim Sequence diversity was accumulated through evolution and breeding process. We developed quadratic code system based on insertion/deletion(indel) markers, each of which is specific to a dense variation block (dvb) with non-random recombination due to many variations. Firstly, 2,351 VBs were mined by analyzing of 40 Korean cultivars and published genome sequences of rice(oryza sativa L. spp japonica) for transferability to dvb-specific InDel markers. Secondly, 12,174 putative InDels in the dvb regions were identified for the development of rice barcode system. A set of 595 PCR-based novel InDel markers was designed in order to widen the genetic basis for national rice breeding programs. Among them, 112 dvb-specific InDels from all rice cultivars were selected by gel electrophoresis, which were converted as 2D barcode types according to comparing amplicon polymorphisms in the eight cultivars to the reference cultivar. Finally, the polymorphism of the markers were assessed in 284 rice cultivars, and the rice barcode system that allows a clear distinction among rice cultivars is also detailed. In addition, the changing of the dvbs in a chromosomal level can be quickly identified by investigating the reshuffling pattern of the rice cultivars with 44 marker sets. These results could provide significant information not only for the crop identification but also for breeding of varieties with minimal screening by using a small number of the selected InDel markers. Acknowledgement: This work was carried out with the support of Research Program for Agriculture Science & Technology Development (Project title: Development of high-quality rice varieties to adaptability in middle-area, Project No. PJ ) Rural Development Administration, Republic of Korea. *Corresponding Author: Tel , parkhm2002@korea.kr 76

119 PA-61 Eun Su Lee *, JinHee Kim, Yul-Kyun Ahn, Jong Pil Hong, Abinaya Manivannan, Tae Sung Park, Minkyoung Kim and Do-Sun Kim Watermelon (Citrullus lanatus) is an important vegetable crop in the world and contains various health promoting compounds including many antioxidants such as lycopene and beta-carotene. Gummy stem blight (Didymella bryoniae (Auersw.)) is one of the most destructive diseases and causes crown blight, defoliation and fruit rot, resulting in severe production loss in watermelon. Single nucleotide polymorphisms (SNPs), important molecular markers for genetic studies are frequent sequence variation in the plant genome. We selected 113 SNP markers using Fluidigm SNP genotyping analysis from two watermelon lines, one susceptible( ) and the other resistant( PI ) to gummy stem blight. We surveyed phenotypes of disease resistance regarding pathogenic fungus (KACC 40937) inoculating F 2 population whose number is 94 samples. Phenotypes consist of 3 types. (1 type : not infection, 2 type : infection, 3 type : severe infection) Genotypes of F 2 samples are identified by utilizing Fluidigm SNP genotyping analysis and were scored as A(P 1 homozygous), H(heterozygous) and B(P 2 homozygous). We calculated genetic distance from SNP markers using Carthagene ver. 1.3 program and genetic linkage map was constructed using F 2 population derived from PI We performed QTLs(quantitative traits loci) using genotype and phenotype data related to resistance index of gummy stem blight disease. Genetic inheritance and QTL analysis indicated that gummy stem blight resistance is controlled by an incompletely dominant effect of major QTLs. *Corresponding Author: Tel , lus4434@korea.kr PA-62 Sang-Choon Lee 1, Won-Kyung Lee 1, Ho Jun Joh 1, Inseo Kim 1, Jaehyeon Jeon 1, Sae Hyun Lee 1, Tae-Jin Yang 1*, Kihwan Song 2* 1 2 Dehydrins (DHNs) are hydrophilic proteins belonging to group II of the late embryogenesis abundant (LEA) protein family. DHNs have highly conserved lysine-rich K-segment and are considered as molecular chaperons playing important roles in plant growth and abiotic stress tolerance. In this study, DHN genes were identified through genome-wide searches in five Cucurbitaceae species including cucumber, wild cucumber, melon, watermelon, and bitter gourd. A total of 32 DHN genes were found in the five species, which were further divided into several types depending on the presence and order of the major conserved motifs such as K-, Y-, and S-segments. In silico expression profiling using RNA-Seq data revealed high expression of SK 3 -type DHN gene and very low expression of other DHN genes in cucumber. In silico promoter analysis identified a number of cis-acting elements related to abiotic stress-response, such as DRE and ABRE, in 2-kb upstream sequences from start codon of four cucumber DHN genes. DHN genes identified in this study will be valuable for understanding stress response mechanism as well as enhancing molecular breeding in Cucurbitaceae crops. Acknowledgement: This work was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through Agri-Bio industry Technology Development Program, funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) (grant number HD0b0). *Corresponding Author: Tel , , tjyang@snu.ac.kr, khsong@sejong.ac.kr 77

120 PA-63 김민주 *, 윤수현, 박재호, 박석만, 진성범 감귤품종중운향과금감속인금감은추위에강해내한성육종프로그램에많이사용되며과피와과육을함께먹을수있는장점이있다. 반면감귤속인길전네이블오렌지는과육의당도가높고신맛이적으며종자가없다는특징이있다. 따라서속이다른두품종간의장단점을보완하면서씨가없고과피와과육을함께먹을수있는감귤품종육성을위하여본연구를수행하였다. 길전네이블오렌지의캘러스와장실금감의잎조직을재료로세포융합을실시하였으며융합된세포를적정배지에서배양하여식물체를얻었다. 얻어진식물체에대한배수성은 Flow cytometry 을이용하여검정하였다. 핵 DNA 를판별할수있는 SSR 마커를이용하여핵 DNA 가금감과길전네이블오렌지의 DNA 를모두포함하고있는이질 4 배체라는것을판별하였다. 또한미토콘드리아와엽록체 DNA 판별마커를이용하여미토콘드리아와엽록체가어느쪽에서유래되었는지확인할수있었다. 두가지품종의핵 DNA 가모두포함된것으로보아향후과실은금감과오렌지의중간형태를나타내는과실특성을나타낼것으로예상되며차후에새로운품종으로활용할수있을것이다. * 주저자 : Tel , 88minju@korea.kr PA-64 정재아 1*, 신학기 2, 김미선 1, 박종택 1 스프레이국화 그린트리 (Green Tree) 는 2007 년국립원예특작과학원화훼과국화육종온실에서녹색폼폰형겹꽃인 Leonardo' 를모본으로연녹색폼폰형겹꽃 Green Bird 를부본으로교배하여종자를채종하였다 년종자를파종, 육묘후실생묘를정식하여자연개화후선발하여 07B1-140 계통번호를부여받았다. 이후 1 차, 2 차특성검정을실시하였으며, 3 차특성검정을실시하였다. 개화특성검정을실시하고촉성재배, 자연재배, 억제재배의주년생산성검정및절화수명등소비자기호성에대해조사되었다. 국화 그린트리 품종은 10 월하순에자연개화하는절화용스프레이추국이다. 화색은 YGN144A 로밝은녹색폼폰형겹꽃으로꽃크기는 4.2±0.2 cm이며화당설상화수는 282.7±10.9 매로볼륨감이있는중형화로디스버드형으로재배도가능하다. 초장은 106.1±1.5 cm로균일하가잘자라고동시에개화되고고온기화색발현이균일한특성을가지고있다. 착화수는약 16.8±2.3 개정도이며절화수명은약 21 일로길다. 단일처리후연평균개화반응은 7.5 주이다. 그린트리 품종의재배시주의할점은고온이지속될경우잎이처리는현상이나타날수있으므로과습되지않도록관수에주의해야한다. 그리고억제재배기개화지연을방지하기위해서화아분화시온도를 18 도이상유지시켜주는것이좋다. * 교신저자 : Tel , jabisung@korea.kr 78

121 PA-65 정재아 *, 이은혜, 신학기, 김미선, 박종택 국화에서흰녹병은상업용재배에서가장심각한피해를주는병으로수출검역대상이기도하다. 하지만전체절화국화재배면적의 80% 를차지하는스탠다드즉대국은현재까지재배되는주요품종중에흰녹병저항성품종이없다. 본실험은국화유전자원중에스프레이국화내병성을도입하기위해교배조합을작성하고교잡후대의저항성유전과국화꽃의주요유전형질에대해조사하였다. 흰녹병저항성유전자원으로는 Dancer 와 Gibaeg 품종을교배친으로이용하였으며스탠다드국화교배친으로는주요한육성계통과상용품종을이용하였다. 후대개체를대상으로 3 차에걸친포자비산법을이용한저항성검정을실시하고흰녹병에대한 1 차선발을실시하였다. 흰녹병에대해서 Jinba Dancer 조합에서는 2:1, Dancer 는 4:1 분리비를보였으며, Gibaeg 조합에서는 2:1 분리비를보였다. 후대실생개체 89 개체중흰녹병저항성인 52 개체를선발하고개화특성에따라스탠다드국화의기본특성에적합한개체를 2 차선발하였다. 선발된후대개체는백색스탠다드국화와다시여교배하였다. 여교배한후대는 482 립을채종하고 258 개체를정식하였으며그중다시흰녹병저항성과개화특성검정을실시하여 50 개체를선발하였다. 현재스프레이내병성유전자를도입한흰녹병저항성유망계통을 3 계통선발하였다. * 교신저자 : Tel , jabisung@korea.kr PA-66 Abinaya Manivannan 1, Yul-Kyun Ahn 1*, Tae-Hwan Jun 2, Eun-Young Yang 1, Sena Choi 1, Jinhee Kim 1, Eun-Su Lee 1 and Do-Sun Kim Rapid advancements in genome sequencing platforms provide ample opportunity for the development of DNA-based molecular markers. The present study deals with the genome wide identification, genotyping, and bioinformatics analysis of single-nucleotide polymorphism (SNP) markers related to powdery mildew (PM) resistance in two pepper varieties. Capsicum baccatum (PRH1- a PM resistant line) and Capsicum annuum (Saengryeg- a PM susceptible line), were re-sequenced to develop the SNP markers. The sequencing resulted in a total of 6,213,009 and 6,840,889 SNPs for PRH1 and Saengryeg respectively. Among the SNPs, 4,887,031 polymorphic SNP loci were identified between the two lines and 306,871 high-resolution melting (HRM) marker primer sets were generated. In order to understand the SNPs associated with the vital genes involved in diseases resistance and stress associated processes, chromosome-wise gene ontology analysis was performed. The gene ontology results revealed the occurrence of SNPs related to diseases resistance genes were predominantly distributed in chromosome 4. Furthermore, the chromosomes 5-8 consisted of SNPs-associated with genes involved in the nucleotide and ion binding processes. From the generated results, HRM primers were selected from each chromosome and evaluated in 46 F4 population of Capsicum. Among the tested primers, 19 primers discriminated the population into resistant, moderate, and susceptible varieties which has been corresponded with the physiological disease evaluation data. Thus, the current endeavor describes a novel approach for the identification of SNP markers that can be utilized for molecular breeding of pepper with powdery mildew resistance. *Corresponding Author: Tel , aykyun@korea.kr 79

122 PA-67 Seon-Hwa Bae 1, Kyeong-Seong Cheon 1, Myoung-Hee Lee 2, Ki-Won Oh 2, Jeong-Hee Lee 3, Ung-Han Yoon 1, Jundae Lee 4, Tae-Ho Kim 1* Perilla is a self-pollinating annual herbaceous plant of the Laminaceae family and has mainly been cultivated as an oil crop in East Asia. Here we report progress on the de novo genome assembly of P. citriodora (2n=2X=20). We undertook a draft de novo genome assembly by combining data from multiple sequencing platforms (Illumina, PacBio RS II) using various libraries with different insertions. The genome was assembled into 1,622 scaffolds with 12,325,979 bp (N50) with a total length of Gb. Ten whole BAC sequences involved in omega-3 biosynthesis pathway were highly covered in the scaffolds. CEGMA showed that the percentage of completeness for this assembly was 92.74% and 97.58% for completely and partially aligned core eukaryotic genes, respectively. BUSCO analysis revealed a completeness score of about 95.5%. Repeat analysis showed that 61.47% of the assembled genome was predicted to be repetitive. A total of 196,413 ab initio gene models were predicted along with Perilla scaffolds using MAKER. Of them, 41,751 gene models were matched at least once with GO, Protein families DB and CCD. A total of 39,025 gene models were predicted along with the Perilla transcripts using Blastx. With the above evidences, we found that 55,418 gene models were involved at least once. GenBank showed that a total of 56,604 gene models were predicted. The results above will provide important information on the genome structure to understand the functional genomics of Perilla. *Corresponding Author: Tel , thkim1961@korea.kr PA-68 Won-Kyung Lee 1, Sang-Choon Lee 1, Ho Jun Joh 1, Inseo Kim 1, Jaehyeon Jeon 1, Sae Hyun Lee 1, Tae-Jin Yang 1*, Kihwan Song 2* 1 2 Superoxide dismutase (SOD), ascorbate peroxidase (APX), and catalase (CAT) are key enzymatic antioxidants which play important roles in plant abiotic stress response and tolerance. In this study, SOD, APX, CAT gene families were identified through genome-wide searches in five Cucurbitaceae species including cucumber, wild cucumber, melon, watermelon, and bitter gourd. As a result, 67, 59, and 27 genes were identified to encode SOD, APX, and CAT, respectively, in the five species. Through domain search, phylogenetic analysis, and in silico subcellular location analysis, features of the genes were characterized. In silico expression profiling using RNA-Seq data revealed diverse expression patterns of SOD, APX, and CAT genes in normal cucumber tissues. Antioxidant enzyme genes identified in this study will be valuable resource for understanding stress response mechanism as well as facilitating molecular breeding of Cucurbitaceae crops. Acknowledgement: This work was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through Agri-Bio industry Technology Development Program, funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) (grant number HD0b0). *Corresponding Author: Tel , , tjyang@snu.ac.kr, khsong@sejong.ac.kr 80

123 PA-69 Yong Bog Kim 1, Rahul Vasudeo Ramekar 2, Byong Gon Choi 1, Ju Kyong Lee 2, Ik Young Choi 3, Sun Gang Choi 3 and Kyong Cheul Park 3* Allium ochotense and A. microdictyon, commonly known as Mountain garlic,' are popular and economically important species in many countries including Korea, China, and Mongolia. Their leaves are often used as side dishes as well as in traditional medicines. In Korea, these two species are at risk of extinction due to damage to their natural habitat, and thus they need to be conserved and the subject of breeding programs. However, their identification relies mostly on morphological data, which have limitations and, until recently, led to classifying these two species under A. victorialis. In the present study, a simple and reliable method of molecular identification was developed, targeting four barcoding regions: the internal transcribed spacer (ITS), the maturase K gene (matk), the chloroplast psba-trnh intergenic region, and the large subunit of the ribulose-bisphosphate carboxylase gene (rbcl). Single nucleotide polymorphisms (SNPs) were found in ITS and matk regions, but species-specific primers were designed based solely on the SNP at position 680 of the ITS region that differentiated A. ochotense from A. microdictyon. Using these primers in amplification refractory mutation system (ARMS)-PCR, A. ochotense, and A. microdictyon were simultaneously and efficiently distinguished. This study is the first to report a simple, rapid, and efficient method for discriminating A. ochotense and A. microdictyon, evidencing the utility of species-specific markers to accelerate conservation and breeding programs. *Corresponding Author: Tel , kyongcheul.park@kangwon.ac.kr PA-70 l Ye-Ji Lee 1,4, Kyeong-Seong Cheon 1, Eun-Ju Byeon 1, Hyeonso Ji 2, Gang-Seob Lee 3, Ung-Han Yoon 1, Nam-In Hyung 4, Tae-Ho Kim 1* Rice (Oryza sativa L.) is one of the major crops for most Asians and grain production has dramatically increased over the past decade. However, severe weather conditions, such as strong winds, often cause yield reduction by stem lodging. To identify the loci related to lodging resistance, we studied QTL analysis using the recombinant inbred lines derived from Milyang23 and Gihobyeo (MGRILs). We generated 3,221 SNP markers from 162 MGRILs through resequencing analysis by Illumina Hiseq Recombination breakpoints were visualized on bin-map with SNPs compared to parental chromosome and used for the construction of the high-resolution genetic map. Subsequently, in 2017, the 12 QTLs in each internode diameter and culm length were detected from within the QTL regions of chromosome 1, 6, and 7 with high accuracy (LOD>8). In comparison with QTL regions of 2016, 11 QTLs were overlapped and new one (LOD>20) was detected in It will be possible to narrow down these candidate QTL regions to identify genes related to lodging resistance by map-based cloning. Acknowledgement: This research was supported by "National Institute of Agricultural Sciences" (Project No. PJ010089), Rural Development Administration, Republic of Korea Corresponding Author: Tel , thkim@rda.go.kr 81

124 PA-71 Su-Min Jo *1, Tae-Heon Kim 1, Dongjin Shin 1, Saes-Beul Lee 1, Ji-Yoon Lee 1, Sang-Ik Han 1, Jun-Hyeon Cho 1, Kyung-Min Kim 2, Jong-Hee Lee 3, You-Chun Song 1, Dong-Soo Park 1, Myung-Kyu Oh Recently drought caused by global climate change is seriously giving a threat to world rice production and food security in rice field. However, little progress has been made in the genetic analysis of drought tolerance, because it is a complex trait controlled by a number of genes and affected by various environmental factors. In here, we screened 218 rice genetic resources for drought tolerance at vegetative stage and selected 32 highly drought-tolerant varieties in greenhouse. Under rain-fed conditions, Nagdong grain yield decreased by 53.3% from 517 kg/10a to 241 kg/10a when compare to irrigation condition. By comparison, Samgang yield decreased by 23.6% from 550 kg/10a to 420 kg/10a. The variety Samgang exhibited strong drought tolerance and stable yield in rain-fed conditions and was selected for further study. To identify QTLs for drought tolerance, we examined visual drought tolerance (VDT) and relative water content (RWC) using a doubled haploid (DH) population consisted of 101 lines derived from a cross between Samgang (a drought tolerance variety) and Nagdong (a drought sensitive variety). Three QTLs for VDT were located on chromosomes 2, 6, and 11, respectively, and explained 41.8% of the total phenotypic variance. qvdt2, flanked by markers RM324 and S2016, explained 8.8% of the phenotypic variance with LOD score of 3.3 and an additive effect of 0.6. qvdt6 was flanked by S6022 and S6023 and explained 12.7% of the phenotypic variance with LOD score of 5.0 and an additive effect of 0.7. qvdt11, flanked by markers RM26765 and RM287, explained 19.9% of the phenotypic variance with LOD score of 7.1 and an additive effect of 1.0. qrwc11 was the only QTL for RWC to be identified; it was in the same locus as qvdt11. qrwc11 explained 19.6% of the phenotypic variance, with a LOD score of 4.0 and an additive effect of 9.7. To determine QTL effects on drought tolerance in rain-fed paddy conditions, seven DH lines were selected according to the number of QTLs they contained. Of the drought tolerance associated QTLs, qvdt2 and qvdt6 did not affect tiller formation, but qvdt11increased tiller number. Tiller formation was most stable when qvdt2 and qvdt11 were combined. DH lines with both of these drought tolerance associated QTLs exhibited the most stable tiller formation. These results suggest that qvdt11 is important for drought tolerance and stable tiller formation under drought stress condition in field. *Corresponding Author: Tel , tnals88319@korea.kr PA-72 김현태 1*, 강범규 1, 한원영 1, 이병원 2, 이영훈 3, 최만수 3, 서정현 1, 고종민 3, 윤홍태 2, 오기원 1, 하태정 3, 곽도연 콩다수확품종 대풍 은뛰어난재배안정성과수량성에도불구하고종자가작고배꼽색이갈색인이유로재배가확대되지못하고있다. 대풍 의이러한종자상의단점을개선하고, 더하여기계수확적응성을향상시키고자시도해온결과 새금 을개발하였기에여기에소개한다. 수원 190 호 와 대원콩 을교배하여육성한대립내재해성인계통을부본으로하여 2003 년에 대풍콩 과교배하였다 년 F 2 집단에서선발한개체를 2006 년아시아채소연구개발센터에서 3 세대세대진전후 2007 년 F 6 세대부터국립식량과학원시험포장에서계통전개하여선발하였다. 2009~2010 년생산력검정시험에서종실색택이좋고다수성이며기계수확적성이높은계통을선발하여 밀양 232 호 의계통명을부여하고, 2011~2013 년 3 년간전국 11 개소에서지역적응성을검정하였다. 새금 은유한신육형으로모용과협색이갈색이고종피와배꼽색은황색이며꽃색은백색이다. 성숙기가 10 월 17 일로 대원콩 보다 3 일정도늦으며, 종실은구형이고 100 립중이 25.4g 으로대원콩보다 1.2g 무거운대립품종이다. 키가대원콩보다크지만표준재배에서의도복정도는대원콩과비슷한정도로강하다. 포장에서의자연이병정도와 8r 균주를이용한분무접종에서불마름병에저항성을보였으며, 콩모자이크바이러스의경우자연이병포장에서는증상이나타나지않았으나 G7H, G6H, G5 strain 의즙액접종에서는모자이크반응을보였다. 내탈립성이강하여시험포장에서탈립이잘되지않으며, 성숙꼬투리를 40 건조기에 48 시간두어도꼬투리가터지지않았다. 도복에강하고, 가장낮은꼬투리의높이가 18cm 로서컴바인등을이용한기계수확이용이한품종으로생각된다. 그러나, 밀식재배에서는도복에약하며, 검은뿌리썩음병이병주율이대원콩보다높다. 두부수율과메주청국장수율및발효적성은대원콩과비슷하였으며, 남부적응지역의평균수량이 3.02ton/ha 로 대원콩 과비슷한수준이다. * 주저자 : Tel , sojatae@korea.kr 82

125 PA-73 Soon Ju Park 1,2, Sebastian Soyk 2, Jong Hyang Bae 3, José M Jiménez-Gómez 4, Zachary B Lippman In many plants, the signals for flower induction depends on seasonal variation in day length. Photoperiod response depends on cross-talk between light perception and the circadian clock, which function together to control expression of the flowering hormone florigen. Here we show that loss of day-length-sensitive flowering in tomato was driven by the florigen paralog and flowering repressor SELF-PRUNING 5G (SP5G). SP5G expression is induced to high levels during long days in wild species, but not in cultivated tomato because of cis-regulatory variation. CRISPR/Cas9-engineered mutations in SP5G cause rapid flowering and enhance the compact determinate growth habit of field tomatoes, resulting in a quick burst of flower production that translates to an early yield. Our findings suggest that pre-existing variation in SP5G facilitated the expansion of cultivated tomato beyond its origin near the equator in South America, and they provide a compelling demonstration of the power of gene editing to rapidly improve yield traits in crop breeding. Corresponding Author: S.J.P. (sjpark75@wku.ac.kr), J.M.J.-G. (jose.jimenez-gomez@versailles.inra.fr) Z.B.L. (lippman@cshl.edu). PA-74 Kyounghee Lee *, Ok-Sun Park & Pil Joon Seo Plant somatic cells can be dedifferentiated by in vitro tissue culture methods, and massive genome-wide chromatin remodeling occurs particularly during callus formation. Since callus resembles lateral root primordium, tissue identity conversion is essentially required when leaf explants are used. Consistent with that differentiation state is defined by chromatin structure that permits limited gene profiles, epigenetic reprogramming would underlie cellular reprogramming for tissue identity changes. Although histone methylation suppressing leaf identity during callus formation has been demonstrated, it is still elusive which epigenetic factor is involved in activation of root identity. Here, we report that JUMONJI C DOMAIN-CONTAINING PROTEIN 30 (JMJ30) stimulates callus formation by promoting expression of a subset of LATERAL ORGAN BOUNDARIES-DOMAIN (LBD) genes that establish lateral root primordia. The JMJ30 protein binds directly to the LBD16 and LBD29 promoters with the help of AUXIN RESPONSE FACTOR 19 (ARF19) and activates expression of LBD16, LBD17, LBD18, and LBD29. In support of this, the JMJ30-deficient mutants displayed reduced callus formation with low transcript levels of LBDs. The ARF-JMJ30 complex catalyzes removal of methyl groups from H3K9me3 especially at the LBD loci to activate their expression. These results indicate that the JMJ30-ARF-LBD axis promotes callus formation. *Corresponding Author: khl0905@skku.edu 83

126 PA-75 Hong Gil Lee 1*, Juyoung Kim 2, Mi Chung Suh 2 and Pil Joon Seo Cuticular wax is an important hydrophobic layer that covers the plant aerial surface. Cuticular wax biosynthesis is shaped by multiple layers of regulation. In particular, a pair of R2R3-type MYB transcription factors, MYB96 and MYB30, are known to be the main participants in cuticular wax accumulation. Here, we report that the MYB30-INTERACTING E3 LIGASE 1 (MIEL1) E3 ubiquitin ligase controls the protein stability of the two MYB transcription factors and thereby wax biosynthesis in Arabidopsis. MIEL1-deficient miel1 mutants exhibit increased wax accumulation in stems, with upregulation of wax biosynthetic genes targeted by MYB96 and MYB30. Genetic analysis reveals that wax accumulation of the miel1 mutant is compromised by myb96 or myb30 mutation, but MYB96 is mainly epistatic to MIEL1, playing a predominant role in cuticular wax deposition. These observations indicate that the MIEL1 MYB96 module is important for balanced cuticular wax biosynthesis in developing inflorescence stems. *Corresponding Author: Mi Chung Suh, mcsuh@chonnam.ac.kr; Pil Joon Seo, pjseo1@skku.edu PA-76 김경민 *1, 신동진 1, 김경훈 1, 박효진 1, 권유리 1, 현종내 1, 오명규 1 1 귀리 (Avena sativa L.) 는세계적으로벼과작물중에서밀, 옥수수, 벼, 보리다음으로생산량이많은작물로대부분이가축사료로쓰이나, 종실은단백질과지질이풍부하고체내이용률이높은식이섬유인베타글루칸이 3~6% 들어있어식용으로도그가치를인정받고있다. 귀리는두가지종류로종실에껍질이있는겉귀리와껍질이없는쌀귀리로구분되는데이런이유로겉귀리는수확후껍질을탈피하기위한노력이필요하지만쌀귀리는탈곡과함께껍질이제거되어식용으로이용하기에유리해국내에서대부분쌀귀리를식용으로이용하고있다. 이에겉귀리와쌀귀리의탈부특성과근연관계가연관성이있는지알아보고자국내에서재배되고있는겉귀리 7 품종과쌀귀리 5 품종총 12 품종을수집하여공시하였다. 겉귀리와쌀귀리의탈부여부는수확후탈곡기를이용해탈곡되어나오는종실의껍질의유무로탈부성을확인하였고, 품종간의근연관계는분자마커를활용하여분석하였다. 분자마커는 University of Sasakatchewan 의 CMG-Lab 에서제공하는 61 개의 SSR 마커를이용하여분석하였고그중 22 개의마커가다형성을나타내었다. 이후 22 개의마커에서확인되는밴드패턴을 NTSYSpc 2.1 프로그램을사용하여 Tree plot 으로나타내어근연관계를확인하였다. 그결과, 탈곡후껍질이벗겨지는탈부특성은겉귀리 7 품종의경우탈부되지않았고, 쌀귀리 5 품종은탈부되는특성을나타내었다. 근연관계는 Coefficient 1.20 에서겉귀리 6 품종과쌀귀리 1 품종, 그리고겉귀리 1 품종과쌀귀리 4 품종 2 개의그룹으로나뉘어졌으며대체로겉귀리와쌀귀리끼리가깝게그룹화되는경향을나타냈다. 이결과를토대로향후더많은다형성마커를이용하여귀리의품종판별에대한기초자료로활용하겠다. * 주저자 : Tel , raiders87@korea.kr 84

127 PA-77 Hyun-Ju Kang 1, Eokkeun Ahn 2, Song Lim Kim 1, Inchan Choi 1, Jeongho Baek 1, Kyung-Hwan Kim 1, Hyeonso Ji 1* 1 2 Gayabyeo has been known to be resistant to various BPH biotypes collected in Korea. With a purpose of mapping genes of Gayabyeo conferring BPH resistance, we developed a F2 population derived from a cross between Gayabyeo and Taebaegbyeo which is a Tongil type BPH susceptible rice variety. Also, we performed whole-genome resequencing of these two varieties. The sequencing yielded bp for Gayabyeo, and bp for Taebaegbyeo. After quality trimming and read mapping onto Nipponbare reference sequence, the final average mapping depth was 27.6 for Gayabyeo and 25.5 for Taebaegbyeo. Totally, 284,501 putative SNPs between Gayabyeo and Taebaegbyeo were detected. Up to now, 96 CAPS (Cleaved Amplified Polymorphic Sequences) markers were developed based on the detected SNPs which were within restriction enzyme recognition sites, and they have been being used for genotyping 180 F2 plants derived from a cross between Gayabyeo and Taebaegbyeo. By comparison of resequencing data of Gayabyeo and the sequences of already reported BPH resistance genes (Bph3, Bph14, BPH18, BPH26), it was revealed that Gayabyeo has Bph3 and BPH26 resistance genes. We will confirm the BPH resistance genes of Gayabyeo by mapping experiment, and are developing selection markers for these genes. *Corresponding Author: Tel , jhs77@korea.kr PA-78 Jun Oh 1, Hyun-Ju Kang 1, Song Lim Kim 1, Jeongho Baek 1, Inchan Choi 1, Kyung-Hwan Kim 1, Hyeonso Ji 1* 1 Dense green leaf mutants can be utilized in discovery of genes regulation leaf growth and development. We found four dense green leaf mutant rice lines which are Ds061942, Ds073406, Ds074081, and Ds among the Ac/Ds insertion mutant population. For genetic analysis, the mutant lines were crossed to their wild type variety, Dongjin. All of F1 plants showed wild type phenotype indicating that the dense green leaf trait was caused by a recessive gene in each mutant line. F2 segregation ratios were also fit to 3:1 (wild type : mutant type), which demonstrated that single recessive gene controls dense green leaf trait in each mutant line. Resequencing of the four mutant lines were performed using Illumina Hiseq 2000 platform Gbp sequence data were produced per mutant line. After quality trimming and read-mapping onto rice reference genome sequence (Nipponbare), Gbp were mapped onto the reference sequence resulting in average mapping depth of By comparison with Dongjin resequencing data, 42,386-58,393 single nucleotide polymorphisms (SNPs) were found between each mutant line and Dongjin. Based on these results map-based cloning of the genes regulating the dense green leaf trait of the mutant lines will be carried out with F2 population derived from crosses between each mutant line and Dongjin. *Corresponding Author: Tel , jhs77@korea.kr 85

128 PA-79 배민지 1, 진영돈 1, 안동춘 1, 정용모 1, 최시림 1, 홍광표 2, 황주천 3* 경남농업기술원화훼연구소에서는 2012 년 10 월에화형과화색이우수한분홍색폼폰형의 Lollipop 을모본, 흰녹병저항성이며착화성이우수한황색폼폰형 Yellow Cap 을부본으로인공교배하여교배조합으로부터 386 개의종자를획득하였다. 그후교잡 1 세대를육묘하여특성검정후화색이선명하고성장세와착화성이우수한 5 개체 (LY13-27, 63, 85, 126, 274) 를 1 차로선발하였다. 이들중특성이우수한개체 LY-85( 경남교 CS-54 호 ) 를삽목에의해개체증식후화훼연구소비닐온실내에정식하였으며, 2014 년부터 2016 년까지 3 년간에걸쳐 1~2 차생육특성검정을통해안정성, 균일성과흰녹병저항성등을조사하였고, 2016 년에는계통번호 경남교 CS-54 호 를부여하여 3 차특성검정을수행해안정성과균일성에대한연차별재현성그리고주년생산성 ( 자연, 촉성, 억제재배 ) 및품평회와시장출하등을통해생산자와소비자의기호성평가를받았다. 그결과기호성이좋고, 화색및화형등품질이우수하다고판단되어 2016 년농작물직무육성신품종심의회심의를거쳐 오렌지팝 (Orange Pop) 으로명명하고국립종자원에품종보호출원하였다. 오렌지팝 은밝은오렌지색 (GON163C) 의폼폰형의겹꽃화형인스프레이국화로착화성이좋고줄기가강건한특징이있다. 자연개화기는 10 월 28 일로대조품종 (11 월 4 일 ) 보다 7 일정도빨리개화하는특징이있으며, 초장은 96.9cm 로대조품종 (92.1cm) 보다길다. 꽃직경은 5.2cm, 꽃자루길이는 11.1cm 이며, 설상화수는 12.2 개정도이다. 절화수명은약 20 일정도이며, 비닐하우스내에서연중재배가가능하다. 재배시초기생육증진을위한영양분관리가중요하며, 하계고온기재배시화색발현을위해한낮에차광율 30% 정도의한랭사로차광을해주고환기를충분히해주는것이좋다. * 주저자 : Tel , baeminji@korea.kr PA-80 Myoung Hee Lee 1, Ki Won Oh 1*, Myung Sik Kim 1, Jung In Kim 1, Eun Young Oh 1, Suk Bok Pae 1, Un Sang Yeo 1, Tae-Ho Kim 2, Jeong Hee Lee 3, Do Yeon Kwak 1, Yong Chul Kim A interspecific cross between P. citriodora and P. hirtella constitutes ideal material for a linkage map construction in genome project of Perilla. The chromosomes of the species are same with n=10 and progenies of the cross are normal in growth and seed set. The phenotype of F2 population of the cross are normally distributed and this is a prof of the high affinity of the chromosomes during their sexual reproduction. Total number of 2,518 markers were mapped in 10 linkage groups with total length of 1,309 cm. Among the markers, 21 QTLs were associated with agronomic traits under LOD 3.0 condition. Purple leaf color related QTL, qtl_lc-1, was mapped in LG3 and two anthocyanin biosynthesis transcription factor like sequences, WD40 repeat-like superfamily protein and myb domain protein 43, were detected near from qtl_lc_1. *Corresponding Author: Tel , ohkw1004@korea.kr 86

129 PA-81 박은영 1, 이정동 1* 1 세계적으로생산되는식물성기름의주요원료중하나인콩은기름구성성분의 23% 를단일불포화지방산인 oleic acid 가차지하고있다. Oleic acid 는높은온도에서기름의안정성을높여주고혈압을조절하는기능이있는것으로조사된바있다. Oleic acid 함량이높은계통을선발하기위해 EMS 를처리한돌연변이집단을육성하였고, M4 세대에서고 oleic acid 함량을보인 PE451(44.4%), PE2742(47.7%), PE3058(33.4%) 을선발하였다. 이들돌연변이계통과 wild type 인풍산나물콩과다수성이며대립품종인우람을인공교배하여얻은 F 2:3 집단의지방산을분석한결과 oleic acid 의변이가관찰되었다. Oleic acid 에서 linoleic acid 로생합성되는과정에 Fad2-1A 유전자와 Fad2-1B 유전자가관여하는것으로알려졌고, 이들유전자가기능을잃으면 oleic acid 함량이높아지는것으로선행연구에서보고되었다. 선발된고 oleic acid 계통들의 Fad2-1A, Fad2-1B 유전자염기서열을풍산나물콩과비교한결과엑손에서는변이가없는것으로확인이되었다. 따라서 oleic acid 함량이높은돌연변이계통에대한 mapping 연구가필요하면이를통해 oleic acid 함량을높이는새로운 genome 영역이나유전자를밝힌다면콩의 oleic acid 함량을높이는데기여할수있을것으로기대된다. * 주저자 : Tel , jdlee@knu.ac.kr PA-82 도재왕 1*, 윤재복 1, 박석진 1 1 고추탄저병은 Colletotrichum spp. 에의해발생하며고추재배에있어서많은피해를주는병중에하나로국내외에서발생하여농가소득의감소를초래하고있다. 이러한탄저병피해를줄이고자약제를이용한화학적방제보다는식품으로서도안전한탄저병저항성품종의육성을시도하였고 AR 탄저박사 를개발하게되었다. 탄저병저항성품종의모계로사용한 8047 계통은 2009 년도에 37 개상용품종의 F 2 분리집단을정식하고 GMS 와 CMS 분자표지를이용하여웅성불임이 GMSK-MSms, CMS-Rfrf 이며원예적특성이우수한개체를선발하고고정하기를반복하였다. 최종적으로 대들보 F 8 세대에서노지포장에서바이러스에강하고원예적특성및조합능력이우수한개체를선발하여 8047 로명명하였다. 탄저병저항성인부계계통의육성은 1998 년부터현재까지국내에서우점병원균인 Colletotrichum. acutatum 균주를사용하였고대만의 AVRDC 에서도입한 PBC81(Capsicum baccatum) 계통을 microinjection 방법으로과실에직접접종하고선발한개체의탄저병저항성을국내주요재배종인 C. annuum(sp26) 계통으로도입하였다. 그러나종간교잡불화합성으로후대진전이어려워배배양을통해 F 1 개체를생산하고 C. annuum(sp26) 계통과여교잡하여 BC 1 F 1 집단을전개하였다. 이후 C. acutatum 균주로기내접종하여저항성인개체를선발하고 BC 1 F 2 에서원예적특성이우수한 C. annuum(h41) 계통에여교잡하여 BC 2F 2 집단을만들고기내접종과포장저항성으로선발하였다. 또한 SP26 과 PBC81 을이용한 BC 1F 1, BC 1F 2 분리집단에서 QTL 분석을통해 C. acutatum 과 C. capsici 저항성과연관된 QTLs 을동정함에따라개발된분자표지로탄저병저항성을보유한개체를선발하여정식하고기내접종을병행하여서탄저병에강한개체를선발하였다. 분자표지와탄저병기내접종및포장저항성으로선발하고세대진전을반복한결과 BC 2F 8 세대에서탄저병저항성이강하고수량성이좋은탄저병저항성부계 계통을선발하였다. 이렇게만들어진두계통으로예비조합을작성하여조합성능검정과농가시교재배시험을거쳐원예적특성이우수하고탄저병에강한 AR 탄저박사 를육성하였다. * 주저자 : Tel , wahng0@honmail.net 87

130 PA-83 Giha Song 1*, Da-Yea Park 1, Nam-Chon Paek 1 1 The mutants show autonomous lesion formation, spontaneous cell death without any pathogen attack and resistance to a pathogen are classified to lesion mimic mutants (LMMs). Through these phenotypes, LMMs were used to study the mechanisms of programmed cell death pathway and response to a pathogen. In this study, the spotted leaf4 (spl4) mutant which is derived from γ -ray irradiation were used to study the spontaneous cell death mechanism. It has been reported that many LMMs its encoding genes were identified but its molecular mechanism of lesion formation and pathogen resistance is still unclear. The reactive oxygen species (ROS) is the product of senescence and ROS can be found near the spots in LMMs during autonomous lesion formation in LMMs even dough it is a developmental stage. Stay-green is the phenomena of delayed senescence and this is the one character that breeders want to achieve. Also, Scientists study this delayed senescence phenotype to elucidate the leaf senescence mechanism. In this study, we analyzed the rice spl4 mutant, which shows autonomous lesion formation on leaf blades, ROS accumulation and shows the stay-green phenotype. The spl4 locus was identified by map-based cloning. This locus encodes a putative microtubule severing protein, spastin. Our data may suggest that the malfunctioning of microtubule severing protein results in pleiotropic phenotypes of autonomous lesion formation, ROS accumulation and delayed senescence in spl4 mutant. *Corresponding Author: rcmavic@snu.ac.kr PA-84 Hyung-Keun Ku 1, Ye Sol Jeong 1,3, Min-Kyoung You 1,2, Dongho Lee 3, Sun-Hyung Lim 4*, Sun-Hwa Ha 1,2* Terpenoid, the largest group of naturally occurring organic chemical compounds, has been taken the steady concern as a target for genetic modulation because of the nutritional and economic significance of these compounds. To identify the regulatory genes of two secondary metabolisms in rice, blue light was selected because it simultaneously affected the highest content of total carotenoids and phenolic compounds when examined in different light treatments of white, blue, red, dark, and green. The seventeen transcription factor (TF) genes were discovered to show the up-regulated pattern in blue light (B) compared to white light (W) through microarray analysis. In particular, a TF13 gene, a member of the MYB transcription factor gene family, showed 2.7 fold higher expression levels in B/W and was selected one of the best candidates via further trans-activation assay of five promoters of anthocyanin and carotenoid biosynthetic genes. In this study, the function of TF13 on terpenoid biosynthesis was only analyzed. Firstly, meta-profiling analysis with TF13 and eight terpenoid biosynthetic genes based on microarray data sets showed dramatic circadian pattern of TF13 in rice leaves and roots at different stages, as well as, the expression patterns induced by ABA, drought, and cold stress conditions. To investigate the functional roles of TF13 in rice plants, we analyzed transgenic plants overexpressing TF13 and the T-DNA activation tagging mutant lines using qrt-pcr. The overexpression of TF13 resulted in the enhanced expression of terpenoid biosynthetic genes. Recently, we are producing the CRISPR/Cas9-induced TF13 KO lines in rice. We hope it might be very helpful to understand the regulation mechanism of plant secondary metabolism. *Corresponding Author: limsh2@korea.kr, sunhwa@khu.ac.kr 88

131 PA-85 Lee Jae Woo *, Cheol Seong Jang Recently, many mutation breeding studies have been used ionizing radiation such X-rays, gamma rays and neutrons in many major crops. Gamma-ray induced mutant rice has been also reported for breeding and genetic studies. However this genome-wide effect is still largely unknown. In this study, we carried out genome-wide effect identification by gamma-ray induced mutant rice. We obtained 3,000 mutant rice lines induced by gamma-rays of 200Gy and 300Gy applied to Donganbyeo (wild-type,wt) After that, we developed up to 8 th generation, Among them we selected a total of 244 core individuals about abiotic stress tolerance and excellent quantitative traits. Among these selected mutants line, we performed whloe genome re-sequencing at abiotic stress tolerance 20 line and excellent quantitative tratis 40 line from an Illumina HiSeq4000 platform. Average 65,421,382 quality-filtered read were generated, with average sequencing depth of ~30X for each line. Fitered reads aligned on to the rice reference genome Nipponbare, its average mapped reads and mapping percentage are 62,739,789 and 95.88% respectively. Single nucleotide polyphisms(snps) and insertions/delections(indels) were detected using Genome Analysis Toolkit(GATK). Average SNPs and 41,752 INDELs were identified. We selected only homozygous variants from a total variants, where significant mutation were average SNPs 85, InDels 131 respectively. The effects of SNPs/InDels were classified four levels HIGH effect MODERATE effect, LOW effect and MODIFIER according to SnpEff. A total HIGH effect SNPs variation were 46 and InDels were 496 respectively. Additionally we will study gene functions for stress tolerance and quantitative traits. *Corresponding Author: Tel , csjang@kangwon.ac.rk PA-86 Yong-Wook Ban 1,2, Taewook Kim 3, Doori Park 1,4, Chanseok Shin 3, Ik-young Choi 1* A plant growth type is one of the major important key to use for economical effect including high yield product in agriculture and industry. We tried to find differently expressed genes to classify and understand the regulation of genes related to plant growth in a mutant, dwarf soybean which are happened in F5 derived from crossing of G. max var. Peking and G. soja var. IT The trancriptome sequencing are generated by Illumina high throughput sequencing platform in each 3 samples normal and dwarf lines, respectively. We found highly significantly up-/down-regulated genes in the comparing of gene expression in normal and dwarf soybeans. There are classified that the genes related to disease and stress responsive showed with the up-regulation in dwarf soybean. Such over-expression of disease resistance and other immune responsive genes could be targeted to understand the gene regulation how the immune genes regulate the response of plant growth. Otherwise, photosynthesis related genes are very low expression in dwarf lines. In plants, sucrose interacts with hormone signals, not only regulates many plant processes, but also affects gene expression of photosynthetic components. Lower concentrations of sucrose reduce the overall metabolism and adversely affect plant growth. In addition, expression of genes related to transport such as SAUR, which is responsible for auxin transport, sucrose-proton symporter, cation transporter were also low. The transcriptome expression and genes classified related plant growth could be useful resource to researchers studying plant growth. *Corresponding Author: Tel , choii@kangwon.ac.kr 89

132 PA-87 안혜림 1,2, 김영미 1, 조아르나 1,2, 권용락 1, 서용원 2, 정지희 1* 1 2 곰취 (Ligularia fischeri) 는국화과의다년초로최근들어산채작물로각광받으면서재배면적이확대되어주요산촌소득종으로자리잡고있다. 본연구에서는곰취에대한유전육종연구에활용할수있는 microsatellite 표지를개발하고적용가능성을평가하였다. 강릉과태백두지역에서채집한곰취 2 개샘플로부터 DNA 를추출하고일루미나 HiSeq2500 플랫폼을이용하여 250-bp PE sequencing 수행하였다. Genome-wide in silico 분석을통해두샘플간 8bp 이상차이를보이는 unique polymorphic microsatellite loci 를탐색하여총 72 개의프라이머쌍을디자인하였다. 강원도강릉 4 개, 태백 3 개집단에서수집한곰취 66 개샘플을이용하여증폭여부및변이특성을검증하여최종적으로다형성을보이고유전자형식별이뚜렷한 7 개 primer 를선발하였다. Primer 별로관찰된대립유전자수는 3-27 개, 총 154 개가확인되었고, 이형접합도관찰치및기대치평균은각각 0.624( ), 0.680( ) 였다. Bayesian cluster 분석결과곰취집단간에비교적뚜렷한차이가확인되어개발된마커는앞으로곰취의집단유전학적연구및분류학적연구에도유용하게활용될수있을것으로기대된다. * 교신저자 : Tel , florajh@korea.kr PA-88 Fahad Ramzan 1, Hyoung Tae Kim 1, Adnan Younis 1,2, Young Kwan Jo 1, Choi Yu Hyeon 1, Ki-Byung Lim 1* 1 2 In Lilium, development of interspecific hybrids imparts the significant traits (colors, resistance to diseases etc.) of the two sections in consequential hybrids. Verification of interspecific hybrids based on the presence of hybrid characteristics or not. In this regard, cytogenetic techniques, genomic in situ hybridization (GISH) has proved to be most precise and applicable approach for genome evaluations of resulting progenies. Chromosome analysis and genomic constitution identification are main objectives in GISH analysis. GISH technique discriminate the maternal as well as paternal genome in interspecific hybrids. In this study, F1 interspecific crosses were made by using Asiatic hybrids as maternal while L. longiflorum cultivars as paternal parent. According to GISH results, interspecific crosses (AL), one progeny of ID number showed equal genomic distribution of both parents i.e. 12 chromosomes of L. longiflorum & 12 chromosomes of Asiatic genome with two A/L genomic recombinations. Progeny (12268) showed 10 Asiatic chromosomes and 14 L. longiflorum chromosomes. Paternal parent genome influence were increased in the resulting progeny, verified the successful interspecific hybridization between two genomic sections. Consequently, GISH analysis confirmed the genomic presence of parents and discriminated the parental genomes in AL hybrids. Acknowledgement: This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (Project No. NRF-2016R1D1A1B ) and regional Subgenebank Support Programme Rural Development Administration, Republic of Korea (No.PJ ) *Corresponding Author: kblim@knu.ac.kr 90

133 PA-89 Yo-Han Yoo 1, Anil Kumar N.C 1, Jong-Chan Park 1, Yun-Shil Gho 1, Sang-Won Lee 1, Gynheung An 1 and Ki-Hong Jung 1* 1 Water deficiencies are one of the most serious challenges to crop productivity. To improve our understanding of soil moisture stress, we performed RNA-Seq analysis using roots from four-week-old rice seedlings grown in soil that had been subjected to drought conditions for 2 to 3 d. In all, 1098 genes were up-regulated in response to soil moisture stress for 3 d, which causes severe damage in root development after recovery, unlikely that of 2 d. We then validated the expression patterns of two candidate genes using a promoter-gus reporter system in planta and monitored the stress response with novel molecular markers. An integrating omics tool, MapMan analysis, indicated that RING box E3 ligases in the ubiquitin-proteasome pathways are significantly stimulated by induced drought. We also analyzed the functions of 66 candidate genes that have been functionally investigated previously, suggesting the primary roles of our candidate genes in resistance or tolerance relating traits including drought tolerance through literature searches besides diverse regulatory roles of our candidate genes for morphological traits or physiological traits. Of these, we used a T-DNA insertional mutant of rice OsPhyB that negatively regulates a plant s degree of tolerance to water deficiencies through the control of total leaf area and stomatal density based on previous finding. Unlike previous result, we found that OsPhyB represses the activity of ascorbate peroxidase and catalase mediating ROS processing machinery required for drought tolerance of roots in soil condition, suggesting the potential significance of remaining uncharacterized candidate genes for manipulating drought tolerance in rice. *Corresponding Author: Tel , khjung2010@khu.ac.kr PA-90 Joon Ki Hong 1, Ki Jin Park 2, Gang-Seob Lee 1, Dool Yi Kim 3, Ju-Kon Kim 4, Seung Bum Lee 1, Eun Jung Suh 1, Kyung-Hwan Kim 1, Yeon-Hee Lee 1* Callus induction and plant regeneration ability of 16 maize genotypes including Korean inbred lines were investigated using 9 to 15 day-old immature zygotic embryos from maize grown in the pots and field culture. Immature zygotic embryos placed on MS medium supplemented with L-proline 0.7 g/l, MES 0.5 g/l, Dicamba 1.5 mg/l, 2,4-D 0.5 mg/l, AgNO 34 mg/l, and Sucrose 20 g/l showed the highest frequency of call us induction. The highest number of shoots was regenerated upon transferring the embryogenic callus to MS medium supplemented with 5 mg/l zeatin. The root formation was observed when shoots were grown on MS medium supplemented with 0.2 mg/l indole-3-butyric acid (IBA). Additionally, immature zygotic embryos from maize grown in the field produced high frequency of plant regeneration, under the same culture conditions above. Fifteen genotypes except one line showed callus induction and shoot regeneration. Among sixteen genotypes tested, H99, B98, HW3, and B73 gave the best plant regeneration. H99 showed the highest number of shoot formation from the primary embryogenic callus. The results suggest that genotypes and growth conditions of maize plants play a very important role for enhancing the embryogenesis competence of immature zygotic embryos. The successful regeneration from immature zygotic embryos of maize inbred lines provides a basis for molecular breeding of new cultivars by genetic transformation. *Corresponding Author: Tel , yhl2222@korea.kr 91

134 PA-91 Mijeong Kim 1, Taegyu Kim 1, Yoonjung Lee 1, Jisu Choi 1, Giwon Cho 1, Joohyun Lee 1* 1 As rice is the model crop, many studies of abiotic stress is using rice. Cold stress, especially at fertility stage, is the affected to rice yield. In order to identify cold stress-related genes at early young microspore stage, genome-wide association study (GWAS) was conducted. Cultivated 110 rice germplasm were moved in the growth chamber under the condition of 12 /RH70%(12h day/12h night when the rice plant was grown in 10 DBH(days before heading). Also, rice plant as control was moved in the green house under condition of 28 /RH70%(12h day/12h night). After 4 days the plants were moved in a greenhouse. The fertility of rice plant were monitored after the grain were fully grown. The most tolerant rice germplasm to cold stress were Cheongdo-Hwayang-12 and IR38 as 63.1 and 61.8 of fertility and the most recessive rice germplasm were Cheongsongangmi as As a result of GWAS with re-sequencing data and fertility after cold treatment germplasm using genome association and prediction integrated tool (GAPIT), the 18 candidated gene is on chromosome 6, 11 and 12. *Corresponding Author: Tel , joohyun00@gmail.com PA-92 Eun-Gyeong Kim 1,3, Ji-Young Son 3, Yeo-Ok, Park 3, Young-Han Lee 3, Doo-Sang Park 3, Youngmi Choi 1, Joonyup Kim 2, Younghoon Park 1,2* Flavonoids play crucial roles in biotic and abiotic stress responses that are tightly associated with quality of fruit crops. Proanthocyanidins (PAs), a class of plant flavonoids, are colorless phenolic oligomers or polymers synthesized from the anthocyanin branch of the flavonoid pathway. Persimmon (Diospyros kaki Thunb.; 2n=6x=90) fruits accumulate large amounts of PAs during fruit development, which often comprise more than 1% fresh weight at maturity that causes strong astringent sensation in the mouth. Although many approaches have been useful to remove the astringency, molecular understanding that underlie the process remains unclear. Here, we examined the transcriptomes of two commercially grown late (Fuyu) and early (Soshu) maturity cultivars to understand the differences that occur during the maturation of persimmon fruit. De novo transcriptome assembly and subsequent analysis were performed for four major stages (i.e., 9 WAB (week after blooming), 12 WAB, 15 WAB and 18 WAB) selected based on physiological changes examined in each cultivar. Average rates of read mapping for eight representative stages of all fruit maturity were 84.59%. Overall, the results of transcriptome data well reflected the phenological and physiological changes such as free sugar content, physical characteristics of fruits, tannin contents unique to each cultivar. Clustering the differentially up- or down-regulated genes more than two-fold over maturation time in each cultivar (e.g., 9 WAB of Fuyu vs 12 WAB of Fuyu) or at maturation time between the cultivars (e.g., 9 WAB of Fuyu vs 9 WAB of Soshu) further revealed distinct expression profiles responsible for the fruit maturity. *Corresponding Author: Tel , ypark@pusan.ac.kr 92

135 PA-93 Swapan Kumar Roy 1, Seong-Woo Cho 2, Soo Jeong Kwon 1, Kabita Sarker 1, Zhanguo Xin 3 and Sun-Hee Woo 1* Copper (Cu) is an important micronutrient for plants growth and development. But it is extremely toxic to plants at high concentration and can inactivate and disturb protein structures. To investigate the molecular alterations that occur in response to copper stress, the present study was performed in ten-day-old Cu-exposed leaves and roots of sorghum seedlings. The growth characteristics were markedly inhibited, and ionic alterations were prominently observed both in the leaves and roots when the seedlings were exposed to different concentrations (0, 100, and 150 µm) of CuSO 4. Using two-dimensional gels with silver staining, a total of 24 prote in spots ( 1.5-fold) from Cu-exposed sorghum leaves were successfully analyzed by MALDI-TOF-TOF mass spectrometry. Of the 24 differentially expressed proteins from Cu-exposed sorghum leaves, 13 proteins were up-regulated, and 11 proteins were down-regulated. In the Cu root proteome, twenty-one (21) protein spots were found to be expressed differentially upon Cu stress, with more than 1.5-fold differences in abundances between the control and Cu-treated samples. Compared with the control seedlings, a total of 10 proteins were increased significantly while 11 proteins were decreased abundances. These findings suggest that the differentially expressed proteins involved in resistance to stress helped Sorghum bicolor to tolerate high levels of Cu. However, reductions in the abundance the translation and synthesis, photosynthesis and energy metabolism related proteins were observed in response to Cu stress. The results of the present study suggest that photosynthesis and energy metabolism was inhibited under oxidative stress caused by elevated Cu stress. The betaine-aldehyde dehydrogenase identified in this investigation displayed dynamic changes in abundance upon Cu stress, and these proteins are associated with carbohydrate metabolism. The enhancement of the antioxidant defense by this BADH enzyme seems to be one of the strategies to reduce the effect of oxidative damage caused by Cu stress. The resulting differences in protein expression patterns together with related morpho-physiological processes suggested that these results could help to elucidate plant adaptation to Cu stress and provide insights into the molecular mechanisms of Cu responses in C 4 plants. *Corresponding Author: Tel , shwoo@chungbuk.ac.kr PA-94 Zhi-Fang Zuo 1, Hana Jeong 1, Hyeon-Jin Sun 2, Mi-Young Park 2, Dae-Hwa Yang 2, Chang-Ho Eun 2, Hong-Gyu Kang 2*, Hyo-Yeon Lee 1,2 1 Plenty of genes are known in regulating the cold response in plants. Arabidopsis thaliana ICE1 (Inducer of CBF Expression1), a bhlh (basic-helix-basic) type transcription factor which binding to the MYC recognition cis-elements in the promoter of CBF3/DREB to regulate the transcription of CBF(Inducer of CBF Expression CRT/DRE Binding Factor) genes during cold stress. Although we know overexpression of ICE1 increased the cold tolerance in Arabidopsis thaliana, the cold resistance mechanism has not been reported in detail. Here, the 1485 bp ICE1 was cloned from A. thaliana and constructed into the binary vector pcambia3301-pubi::ice1. After plant transformed by the floral dip transformation method, the transcription level in T 1 generation and segregation of T2 transgenic plants analysis of 7 PPT resistance lines suggested that ICE1 had been successfully integrated into T 2-3 and T 2-5transgenic Arabidopsis genome with single copy. The overexpression of ICE1 in T 3-3 and T 3-5 showed big leaves, higher proline content, less MDA content, higher activities of superoxide dismutase (SOD), peroxidase (POD) at low temperatures. This is the detailed reason of overexpression of ICE1 can increase the cold tolerance of transgenic Arabidopsis during chilling and freezing. Acknowledgement: This research was funded by Bio Green 21 Program of Rural Development Administration (PJ011280, PJ011860). *Corresponding Author: honggyu@jejunu.ac.kr 93

136 PA-95 Swapan Kumar Roy 1, Seong-Woo Cho 2, Soo Jeong Kwon 1, Kabita Sarker 1, Zhanguo Xin 3 and Sun-Hee Woo 1* Cadmium (Cd) stress may cause serious morphological and physiological abnormalities in addition to altering the proteome in plants. The present study was performed to explore Cd-induced morpho-physiological alterations and their potentiality associated mechanisms in Sorghum bicolor leaves and roots at the protein level. The most significant growth inhibition was observed in plants treated with a combination of the highest concentrations of Cd 2+ ions (150 μ M). The accumulated level of Cd was significantly increased in plants exposed to the high concentration of Cd (150 µm) compared to the untreated plants. The concentrations of Zn 2+ and Ca 2+ ions were decreased, whereas the Fe 2+ ion concentration was increased when seedling leaves were exposed to Cd 2+. Under Cd stress, quantitative image analysis revealed a total of 33 protein spots that exhibited more than a 1. 5-fold changes in intensity and the 33 differentially expressed proteins were successfully analyzed using MALDI-TOF/TOF MS analysis. Among these proteins, a total of 15 proteins showed increased expression, and 18 proteins showed decreased expression in the treated samples compared to their levels in untreated seedlings. On the other hand, a total of 86 differentially changed proteins were identified at 15-day-old sorghum roots between Cd stress and control conditions (p<0.05) whereas 39 increased and 45 decreased based on 3 biological replicated MaxQuant computational platform. The protein abundance involved in glycolysis and tricarboxylic acid cycle, including glyceraldehyde 3-phosphate dehydrogenase was changed in in sorghum roots after Cd treatment. Cd toxicity may significantly alter the glycolytic pathway and the Cd-induced disorganization of the photosynthetic apparatus, and these effects may have a major impact on the plant s ability to withstand this type of stress. The up-regulation of GADPH protein may play an active role in supplying energy to Cd-treated plants via the glycolytic pathway. Cd has a strongly negative impact on proteins related to carbohydrate metabolism and that could limit ATP production, limit primary carbon metabolism, hamper photosynthetic machineries and inhibit plant growth and development. The observed down-regulation of this protein indicates that carbon fixation is inhibited in Cd-exposed plants, and these results suggest that photosynthesis machineries are also susceptible to Cd stress. On the other hand, the increased abundance of stress-related proteins (GSTs and CYPs) was strongly induced by Cd exposure that observed in the present study that involved in antioxidant defense and detoxification systems. *Corresponding Author: Tel , shwoo@chungbuk.ac.kr PA-96 정하나 2, 좌지방 2, 선현진 1, 양대화 1, 강홍규 1*, 이효연 1,2 1 2 유전자변형작물의상업화를위해서는유전자변형작물의안전성과환경에미치는영향에관한평가가이루어져야한다. 본연구는제초제저항성유전자변형들잔디 JG21 와금잔디 (Z. meliloti) l) 의종간인공수분을통해육성된제초제저항성교배종잔디계통 (JG21-MJ) 의분자생물학적인분석을통해유전자변형작물의환경위해성평가를수행하였다. 제초제저항성교배종잔디들에대한후대안정성을분석하기위하여도입유전자에대한복수세대의 genomic Southern blot 분석과 T-DNA 의게놈내의인접서열을분석하였고, 도입유전자의복수세대별발현을확인하였다. T-DNA 의게놈내삽입인접서열을바탕으로제초제저항성들잔디이벤트 (JG21) 와교배종잔디 (JG21-MJ) 를동정할수있는프라이머를제작하였고, 이를이용한검정방법을수립하였다. 본연구결과로환경위해성평가를위한제초제저항성교배종잔디의분자생물학적인자료를획득하였으며, 계통특이프라이머를제초제저항성유전자변형들잔디이벤트 JG21 과교배종잔디계통의검출을위하여유용하게사용할수있음을확인하였다. 제초제저항성교배잔디의복수세대에대한후대안정성을 Southern blot 과 T-DNA 의게놈내의인접서열을분석한결과, 제초제저항성교배종잔디의 F1 ~ F2 세대에서는도입된모든유전자들이안정적으로도입되어있으며, backbone DNA 는제초제저항성들잔디이벤트 JG21 과교배종잔디계통 (JG21-MJ) 에삽입되지않았음을확인하였고, qrt-pcr 을통해복수세대에서제초제저항성교배종잔디에서목적유전자들이안정적으로발현되고있음을확인하였다. 사사 : 본연구는농촌진흥청차세대바이오그린 21 프로그램 (PJ ) 과농림축산식품부농림수산식품기술기획평가원농림축산식품부사업 ( 과제번호 : ) 의지원에의해수행되었음. * 교신저자 : Tel , honggyu@jejunu.ac.kr 94

137 PA-97 하건수 1*, 송윤호 1, 조윤상 1, 이지우 1 1 소립검정콩은정선등여러지역에서쥐눈이콩으로불리며대립콩에비하여특수하게이용되고있다. 따라서최근소비자들이선호하는소립검정콩에서비린내를제거하고자하였으며, 육성모본으로는비린내유발효소가제거된진품콩 2 호와소립검정콩인다원콩을이용하였다. 비린내유발효소의결핍여부를검정하기위하여효소검정방법과전기영동법을이용하였고, 또한지방종소립겸정콩인쥐눈이콩의도복, 바이러스병해등내재해성을보완하기위하여내도복성, 내병충성, 성숙기및수량성을육성지표로하였다. 특히전통적인이용목적인약용콩으로서생리활성성분함유정도를육성지표로하였다 년인공교배하여 2000 년 F 1 을양성하였으며, 년도에 F 2 F 4 세대를경과하였다. F 5 세대를경과하며 GSL B 계통을선발하였으며, 년도에실시한생산력검정시험에서생육특성이우수하고소립다수성이며내재해성계통으로비린내유발효소가결핍된 강원 152 호 를육성하였다. 강원 152 호 는안토시아닌함량이 16,975µg/ 종피 g 이고이소플라본함량이 2,437/µg/g 으로생리활성성분함량이많은기능성소립검정콩계통이다. * 주저자 : Tel , redclover@korea.kr PA-98 Minjung Yook, Soo-Hyun Lim, Hyejin Yu, Do-Soon Kim * Abiotic stress tolerance is the essential trait for second-generation bioenergy crops which compete with food/feed crops for farmlands. Miscanthus is generally known for its high abiotic stress tolerance, but has not been much studied. To cultivate Miscanthus in a marginal land including salt-stressed area, we need to understand the response mechanism to salt stress and how salt tolerance can be improved. Therefore, in this study, we performed transcriptome analysis between two M. sinensis accessions with contrasting salt tolerance, M119 (sensitive) and M131 (tolerant). The highest and second highest ligule leaves of M119 and M131 were harvested at 0, 24, and 48 hours after salt treatment for RNA sequencing. During the time-course, a total of 363 DEGs were obtained from the pair-wise comparison of the six cdna libraries. For salt-sensitive accession, 143 genes were up-regulated during the first 24 hours after salt treatment, and keep the state of regulation in the subsequent 24 hours. For salt-tolerant accession, 178 genes were sharply up-regulated during the first 24 hours and turned to the normal state in the subsequent 24 hours. Particularly, 67 DEGs (48 annotated) were exclusively up-regulated in salt-tolerant accession and most of them were associated with signal transduction pathway to abiotic stresses in plants including transcription factors and receptor-like kinases. Thus, our results suggest that gene expression regulations of the upstream genes in the salt-tolerance cascades brought about diverse adaptability for salt stress in M. sinensis. The result of this study could be utilized for studying and improving salt stress tolerance in Miscanthus. Acknowledgement: This work was carried out with the support of "Next-Generation BioGreen21 Program for Agriculture & Technology Development (Project No. PJ )", Rural Development Administration, Republic of Korea. *Corresponding Author: Tel , dosoonkim@snu.ac.kr 95

138 PA-99 Yurry Um 1, Dae Hui Chung 1, Gwang Hun Park 1, Jinsu Gil 2, Yi Lee 2, Chungryul Jung 1, Kwon Seok Jeon 1, ManJo Kim 1, Yunmi Park 1* 1 2 Angelica gigas Nakai has been an important herbal medicine for women in Eastern Asia for a long time. However, the plant lack efficient information for genetics. In this study, we developed genomic simple sequence repeat (SSR) markers from the Illumina sequence reads of five A. gigas accessions using next generation sequencing (NGS) technology for molecular breeding base construction of A. gigas. We discovered 138,113 SSRs, motif types of di- to deca-nucleotides, from the 356,018,143 bp repeat regions of the genome of A. gigas. A total of 16,496 primer sets were successfully designed from the 138,113 SSR regions. From these candidates, we selected 848 SSR markers that show in silico polymorphism and we tested 23 tri-nucleotide motif type SSR primer sets for polymorphism using 30 A. gigas accessions. Twenty-one SSR markers showed high PIC value (PIC > 0.6) and we analyzed the genetic relationship of the 30 A. gigas accessions based on the 23 polymorphic SSR markers and constructed phylogenetic trees. These newly developed A. gigas SSR markers would be useful tools for molecular genetics, genotype identification, genetic mapping, molecular breeding, and species relationships study within the Angelica genus. *Corresponding Author: Tel , pym5250@korea.kr PA-100 Ye Sol Jeong 1,2, Young Joo Jung 1, Hyung-Keun Ku 1,3, Jae Kwang Kim 4, Kyoung Bok Lee 4, Mi Ran Ko 1,3, Min Kyoung You 1,3, Sun-Hyung Lim 5, Ju-Kon Kim 6, Dong ho Lee 2, Sun-Hwa Ha 1,3* The β -carotene biofortified rice showing golden color phenotype has been developed via bicistronic expression using a 2A sequence from foot and mouth disease virus (FMDV) that was known for a mammalian pathogenic virus. As a substitute for this FMDV-2A (F2A, 20 aa), 2A peptides of non-mammalian virus-origins, T2A (20 aa) from Thosea asigna virus and I2A2 (30 aa) from Infectious myonecrosis virus, were chosen considering of the reported cleavage efficiency. Their synthetic DNA sequences using rice codon usage were in-frame embedded between two synthetic genes for β -carotene production to generate two recombinant genes, stptac (stpsy-t2a-stcrti) and stpiac (stpsy-i2a2-stcrti), respectively. Both transgenic rice plants displayed golden color in seed endosperms. Three independent lines being verified the single copy-insertion of T-DNA were selected for transgene analysis, respectively. Total amounts of carotenoids in stptac were an average of 1.66 μ g/g, which were 2.1-fold higher value than stpiac seeds (0.81 μ g/g). Gene expression patterns of stpsy and stcrti were similarly showed the highest level among stptac and the lowest level among stpiac lines. Immunoblot analysis showed higher expression of proteins in stptac than stpiac lines. None of large recombinant proteins was detected as a linked form of stpsy and stcrti by T2A or I2A2 unlike F2A, demonstrating both T2A and I2A2 peptides are good system to simultaneously express two genes with better efficiency than a F2A peptide in plant system. In addition, highly elevated zeaxanthin level in stptac line 1 were identified by the integration of T-DNA into 1 st intron region of zeaxanthin epoxidase (ZEP). As a result, Zep expression and conversion of zeaxanthin into antheraxanthin were entirely shut down. *Corresponding Author: Tel , sunhwa@khu.ac.kr 96

139 PA-101 Hwan-Hee Bae *, Beom-Young Son, Jung-Tae Kim, Jin-Seok Lee, Sang Gon Kim, Seong-Bum Baek To date, many researches have been done for Quality protein maize (QPM). But there is few maize QPM varieties in Korea. In this reason, to develop maize QPM cultivars, investigation of the growth characteristics and amino acid composition among QPM lines was carried out. The range of siliking days was range from 60 to 69 days. In plant height, CML492 was shortest (152 cm) and CML143 was the tallest (295 cm). The ear height of CML492 was 66 cm and that of CML143 was 175 cm. Protein contents of maize QPM grains were from 9.07% to 12.67%. CML556 was the highest and CML493 was the lowest among maize QPM grains. The contents of fat were from 3.38% to 5.25%. The lowest and highest were CML141 and CML147, respectively. The contents of ash ranged from 1.22% to 1.78%. CML555 was the lowest and CML142 was the highest. carbohydrate contents were from 71.7% to 75.5%. CML557 was lowest and CML493 was the highest. The calories of each line ranged from 346 to 365 Kcal, CML140 was the lowest and CML492 was the highest. The composition of major amino acid in maize QPM grain is glutamic acid (16.34%), and proline (9.80%). The mean of lysine composition was 3.64%, and CML140 showed the highest 4.34%. It is anticipated that domestic adaptive QPM varieties will be developed through back-crossing with domestic maize lines, because CML 140, 141 and 493 contain more than 4% lysine. Acknowledgement: Supported by a project grant from Cooperative Research Program for Agricultural Science & Technology Development (Project title: Selection of RDA developed crop varieties adaptable to northern region of the Korea Peninsula, Project No. PJ ) Rural Developing Administration, Republic of Korea *Corresponding Author: Tel , miami9@korea.kr PA-102 최현모, 배은경, 이효신, 최영임 * GASA (Gibberelic Acid Stimulated transcript in Arabdidopsis) 는식물호르몬인지베렐린에의해조절되는식물유전자로서식물의발달및생장, 그리고다양한스트레스반응에관여하는것으로알려져있다. 토마토를비롯하여애기장대와포도, 포플러에이르기까지다양한식물종에보존되어있고여러식물에서유전자의특성구명연구가이루어지고있다. 그러나아직까지임목에서는 GASA 에관한연구가거의이루어지지않은실정이다. 본연구에서는 GASA 유전자를현사시나무 (Populus alba P. glandulosa) 에서분리하여이를 PagGASA 라명명하였고유전자의구조와발현특성을조사하였다. PagGASA 단백질은 95 개의아미노산으로구성되었으며 N- 말단에 signal peptide 영역과 C- 말단에 12 개시스테인반복서열이보존되어있다. 유전자발현분석결과, PagGASA 는꽃과뿌리에서높게발현하였고, 지베렐린뿐아니라앱시스산, 자스몬산, 살리실산과같은스트레스관련식물호르몬에의하여발현이증가하는것이확인되었다. 또한 PagGASA 유전자발현을증가시키는형질전환현사시나무를제작하여단수조건에서의일별광화학효율을측정한결과형질전환현사시나무가건조내성에효과가있음을확인하였다. 따라서 PagGASA 는스트레스반응성호르몬들에의한신호전달계에매개되어내건성기작에서역할을할것으로보이며, 기후변화대응임목개발을위한분자육종의주요대상유전자로서가치를보여줄것으로기대된다. * 주저자 : Tel , yichoi99@korea.kr 97

140 PA-103 So-Young Kim, Min Kang, Hye-min Lim, Jung-Il Cho, Hyeon-so Ji, Kang-Hyun Lee, Soo-Chul Park, Gang-Seob Lee * We have generated 383 independent transgenic lines that contained the 149 PsGPD (Glyceraldehyde-3-Phosphate Dehydrogenase), 118 ArCspA (Cold Shock Protein), 44 BrTSR15 (Triple Stress Resistance 15) and BrTSR53 (Triple Stress Resistance 53) genes under the constrol of a constitutive (CaMV 35S) promoter to generater genetically modified (GM) rice. TaqMan copy number assay was determined inserted T-DNA copy number. Also FSTs analysis was isolated from 203 single copy T-DNA lines of transgenic plants and sequence mapped to the rice chromosomes. In analyzing single copy lines, we identified 95 FSTs, among which 37 (38.9%) were integrated into genic regions and 58 (61.1%) into intergenic regions. About 27 homozygous lines were obtained through multi-generations of planting, resistance screening and TaqMan copy number assay. To investigate the transgenes expression patterns, the mrna transcripts of homozygous lines were examined by quantitative real-time PCR analysis from leaf tissue. The transcript levels were significantly increased in all of the transgenic plants compared with wild-type. In several lines of myc-tagged BrTSR15 and BrTSR53 transgenic plants, the BrTSR15 and BrTSR53 proteins were highly expressed in mature leaves. These results may be useful for the large-scale production of transgenic plants or T-DNA inserted rice mutants. *Corresponding Author: Tel , kangslee@korea.kr PA-104 Min Jung Kim 1, Van Binh Nguyen 1, Vo Ngoc Linh Giang 1, Yun Sun Lee 1, Tae-Jin Yang 1* 1 Ginseng (Panax ginseng) is one of the most promising medicinal plant due to its pharmacologically valuable ginsenosides for human health. Cultivation of ginseng takes about 4-7 years with extensive efforts to control quality from biotic and abiotic factors. To overcome this difficulty, an in vitro tissue culture system has been developed to produce biomass of adventitious root. However, this method normally goes with plant hormone application which is toxic or not good for human health. In order to overcome this limitation, we developed an efficient system for ginsenoside production through hairy root biomass mediated by Agrobacterium rhizogenes transformation. Callus tissues of five P. ginseng cultivars and an F 1 hybrid species (P. quinquefolius x P. ginseng) have been investigated for transformation efficiency. Genomic PCR analysis of hairy roots confirmed that rolb and rolc were introduced in six transformed hairy root lines but not in normal untransformed adventitious roots. Especially, these transformed lines grew well in SH basic salt medium without any plant hormones. Taken together, our study will contribute to mass production of ginseng roots with high ginsenosides in the future. Acknowledgement: This work was carried out with the support of "Cooperative Research Program for Agriculture Science & Technology Development (PJ )" Rual Development Administration, Republic of Korea *Corresponding Author: Tel , tjyang@snu.ac.kr 98

141 PA-105 Min Kang, So-Young Kim, Hye-min Lim, Jung-Il Cho, Hyeon-so Ji, Kang-Hyun Lee, Soo-Chul Park, Gang-Seob Lee * Crops are exposed to various environmental stresses. These have been affecting the growth of crops, resulting in the severe loss of agronomic production in many countries. Therefore, development of new varieties of resistant crops is required to assure the desired productivity of crops in stress conditions. In this study, a putatively stress-related gene BrTSR53 was isolated from Brassica rapa. The BrTSR53 is 481 bp long and contains ORF region of 234 bp. This ORF showed strong sequence similarities to the uncharacterized genes from Arabidopsis. The expression of BrTSR53 was determined by quantitative real-time PCR analysis. After 3 hr, the highest quantities of mrna were revealed in cold and salt stress treatments. In drought stress treatments, there was the highest expression after 36 hr. Therefore, it was confirmed that the ORF in BrTSR53 should be a gene that confer increased resistance to B. rapa growing in different stress conditions. The ORF region of BrTSR53 gene was cloned into an expression vector, pyes-dest52, and a new protein with molecular weight of 13 kda was detected by western blot analysis. Also, stress tolerance tests showed that BrTSR53-ORF transgenic yeast exhibited increased resistance to the salt stresses compared with the control. In conclusion, the present data predicts that novel ORF in BrTSR53 can serve as an important genetic resource for abiotic stress resistance. *Corresponding Author: Tel , kangslee@korea.kr PA-106 김동용 1, 박소연 2, 왕헝 2, 권순욱 춘란 (Cymbidium goeringii) 은잎이작고내한성이강하여심비디움류중가장북쪽까지분포되어있는종이다. 한국, 일본, 중국의춘란은 3~4 월에개화하며반음지성식물이다. 중국등주요난소비자들은크기가큰동양란을기피하고향기가있는중소형춘란을선호하고있으나, 이에부합하는품종연구가미흡하고, 여전히자연돌연변이개체들이재배되고있는실정이다. 새만금생명공학센터에서개발된 삼국향 품종은잎에진녹색바탕에설백복륜이강하게발현되고, 원판복륜화로향기를갖는품종이다. 또한잎의길이는 18cm 정도의소형종이고, 잎폭은 1cm 정도로권엽성을보인다. 신품종 삼국향 은향기와무늬를갖춘특유성을가진품종으로서수출용으로경쟁력이높을것으로기대된다. 주저자 : Tel , kdy5724@naver.com 99

142 PA-107 Inseo Kim 1, Yun Sun Lee 1, Jee Young Park 1, Ho Jun Joh 1, Hyun Oh Lee 2, Sang-Choon Lee 1, Tae-Jin Yang 1* 1 2 Rhus chinensis is a woody plant that is distributed throughout the tropical and temperate regions. Its remedial components have enabled R. chinensis to be used as a medicinal herb and for ecological restorations. However, a wide distribution of R. chinensis produced several geographically isolated populations that led to intraspecific diversity. Although R. chinensis have several ecological values, few studies have been conducted at the intraspecific level to classify populations for adequate use of the plants. In this study, a chloroplast genome of Chinese R. chinensis was generated through de novo assembly method. The length of chloroplast genome was 149,094 bp, which encoded 77 protein coding genes, 30 trnas genes, and 4 ribosomal RNA genes. Sequence comparisons to the reported Korean R. chinensis revealed 104 InDels and 463 SNPs between the two chloroplast genomes. These variations were confirmed by phylogenetic analysis conducted using 8 species from the order Sapindales. Then, three molecular markers were developed that could discriminate between Chinese R. chinensis and Korean R. chinensis successfully. As a result, we presume that the markers could be a suitable tool to distinguish different collections of R. chinensis. Ultimately, this research could be a basic data not only for further research of R. chinensis related to remedial effect that could lead to proper use as medicinal herbs but also for ecological restorations of Korean R. chinensis species through selection and breeding of local populations in Yang-gu that is known to be well preserved nature in Korea. Acknowledgement: This work was supported by the Korea Environmental Industry & Technology Institute (Project No.: ) and the Bio & Medical Technology Development Program of the NRF funded by the Korean government, MSIP (NRF-2015M3A9A ). *Corresponding Author: Tel , , tjyang@snu.ac.kr PA-108 Jeong Eun Kim 1*, Hee Ju Yoo 1, Byung-Cheorl Kang 2 and Je Min Lee 1* 1 2 Carotenoids are plant pigments that play a major role in conferring fruit color. Carotenoids or color variations are often controlled by genetic variation of the biosynthetic genes. The color of mature pepper fruit is mainly classified as red, orange, and yellow. Orange and yellow fruit colors are determined by mutations of phytoene synthase (Psy) and capsanthin-capsorubin synthase (Ccs), respectively. Unlike the previous fruit color model, we hypothesized that genetic variation in Ccs controls the orange fruit color in pepper. Ripe fruit of Capsicum annuum K is orange, and its carotenoid profile obtained by HPLC analysis showed a lack of the major pepper carotenoid capsanthin but an abundance of lutein, zeaxanthin, and β -carotene as compared to red pepper. cdna cloning and sequencing analysis detected a new nonsense mutation due to T 1026 insertion in the cdna of Ccs but no DNA variation in Psy. We developed a derived cleaved amplified polymorphic sequence (dcaps) marker to distinguish the nonsense mutation on Ccs. Genetic analysis of the F 2 population derived from C. annuum Sweet Banana (red fruit color) C. annuum K revealed that orange fruit color was determined by a single recessive gene. The nonsense mutation on Ccs distinguished by the dcaps marker was co-segregated with orange fruit color in the F 2 population. The germplasm coupled with the dcaps marker and carotenoid profiling will facilitate marker assisted breeding to select orange fruit color and improve lutein, zeaxanthin, and β -carotene levels in pepper. *Corresponding Author: Tel , jemin@knu.ac.kr 100

143 PA-109 Hee Ju Yoo 1, Gyu-Myung Lee 1, Chang-Sik Oh 2, Inhwa Yeam 3, Je Min Lee 1* Carotenoids are essential for plant and animal nutrition, and are important factors in the variation of pigmentation in fruit, leaf, and flower. Tomato is a model crop for studying the biology and biotechnology of fleshy fruits, and especially for the understanding of carotenoid biosynthesis. In commercial cultivars and germplasms of tomato, visual phenotyping of the colors of ripe fruits could be done easily. However, subsequent analysis of metabolic profiling should be demanded for hypothesizing genetic factors prior to time-consuming genetic analysis. We used high performance liquid chromatography (HPLC), employing C 30 reverse-phase column, to efficiently resolve six carotenoids and isomers of several carotenoids in yellow, orange, and red colored ripe tomatoes. High content of lycopene was detected in red tomatoes. The orange tomatoes contained three dominant carotenoids, namely, δ -carotene, β -carotene, and prolycopene. The yellow tomatoes showed low levels of carotenoids compared to red or orange tomatoes. Based on the HPLC profiles, genes responsible for overproducing δ -carotene and prolycopene were described as lycopene ε -cyclase and carotenoid isomerase, respectively. Subsequent genetic analysis using DNA markers in segregating population and germplasms were conducted to confirm the hypothesis. This study establishes the usefulness of metabolic profiling for inferring the genetic determinants of fruit color. *Corresponding Author: Tel , jemin@knu.ac.kr PA-110 Gyu-Myung Lee 1*, Hee Ju Yoo 1, InhwaYeam 3, DoilChoi 2, Je Min Lee 1* Pepper and tomato provide suitable models for comparisons of fruit ripening processes. We present distinct molecular patterns of ripening between pepper and tomato by transcriptomic analysis. Our analyses unveil potential mechanisms of non-climacteric ripening and pepper-specific pigmentation due to defect of regulators and ethylene synthesis. Functional analysis of ripening regulators by virus induced gene silencing in pepper fruits are in progress. The Golden-like 2 transcription factor is shown to distinct expression pattern rationalizing differential ripening pattern. The gene silencing effect of the Golden-like 2 in pepper fruit is analogous to the uniform mutation. The integrated analysis allows us to better understand differential genetic factors of fruit development and ripening in pepper and tomato. *Corresponding Author: codelkm@naver.com 101

144 PA-111 김재희, 박영기 *, 김세현 추위에강하고대립다수확성호두나무신품종을육성하기위하여본연구를수행하였다. 연구결과를요약하면 1983년에경기도광주에서선발한 산성4호 ( ) 와미국에서도입한 레이크 ( ) 를인공교배하여얻어진종자를파종하여교잡종차대묘를양성하여수확량이많고우량개체를대상으로재배안정성검정을실시한후품질이우수한 SL4-7 클론을 레이크볼 이라명명하였다. 또한 1982년에미국에서도입한 맥킨스터 ( ) 와내한성이우수한 상촌 ( ) 을인공교배하여교잡종차대묘를양성하여품질이우수한 MS2-15 클론을 한아름 이라고최종명명하였다. 신품종 레이크볼 과실의종경과횡경은각각 45.3mm와 42.5mm, 입중과인중비는 17.6g과 52.8% 였으며, 과피두께는 1.1mm이다. 한아름 과실의종경과횡경은각각 46.1mm와 38.8mm, 입중과인중비는 14.6g과 59.6%, 과피두께는 1.0mm로과실형질이일반종호두나무보다우수하였다. 이품종들은 2016년신품종보호출원 ( 레이크볼 : , 한아름 : ) 을하였다. * 교신저자 : Tel , woodpark@korea.kr PA-112 박영기 *, 김재희, 김세현 호두나무는호두나무과 (Juglandaceae) 에속하는낙엽교목으로우리나라에서연평균 12 정도범위인지역에서안정적인생장을한다. 본연구는고품질다수확성호두나무품종을육성하기위해호두나무주산지를대상으로병충해가없고수세가강하며, 과실형질이우수한우량개체들을선발하였다. 선발된우량개체후보목들은국립산림과학원재배시험포지에클론보존원을조성하였고, 개화, 결실된클론을대상으로풍매검정을통한호두나무특성을검정한결과형질이우수한 NM2 개체를선발하였다. 우량개체로선발된 NM2 는 2011년부터 2013년까지결실및과실특성에대한안정성검정을실시한후특성이안정적으로발현되어 골든볼 로최종명명하였다. 신품종 골든볼 과실의종경과횡경은 41.1mm와 40.1mm, 입중과인중비는각각 16.8g, 41.3% 였으며, 과피두께는 1.8mm였다. 이품종은 2014년신품종보호출원을하였다. * 교신저자 : Tel , woodpark@korea.kr 102

145 PA-113 Sun Ha Kim, Kyu-Chan Shim, Hyun-Sook Lee, Anh Quynh Le, Sang-Nag Ahn * Seed germination is being delayed or inhibited under several kinds of stress such as temperature, salt, and osmotic pressure. In direct-seeded rice method, low-temperature germinability is considered as one of the factors for stable stand establishment at temperate regions and high altitude areas. The qltg1 is a major quantitative trait locus (QTL) that plays a vital role in controlling tolerance to low temperature at seed germination stage (also termed as low-temperature germinability). To assess the function of qltg1, a population derived from the cross between Oryza rufipogon (Rufi) and the Korean japonica cultivar, Hwaseongbyeo (HS) was evaluated. A BC 3 F 7 line (TR5) showed better low-temperature germinability than its recurrent parent. The Rufi allele enhanced the low-temperature germination rate, which accounted for 55.4% of total phenotypic variation. The qltg1, including the 12 genes, was detected and located in RM10310-CRM14 on chromosome 1 within 68.2 kb region. To identify the genes targeted by qltg1, the expression profiles of the identified candidate genes and germination behavior of qltg1 under different stress conditions were further investigated and compared to HS, Rufi, and TR5. Increased germinability was observed in TR5 and Rufi than HS at 13 C, 250 mm NaCl, and 500 mm mannitol conditions. These results confirmed that qltg1 showed tolerance for several abiotic stresses such as salt, drought and low temperature. Furthermore, transgenic rice plants will also be developed to conduct a detailed investigation on low-temperature germinability. *Corresponding Author: ahnsn@cnu.ac.kr PA-114 Ji Yun Lee 1*, Hong Jib Choi 1, Chang Suk Huh 1, Jeong Suk Bae 1, Se Jong Kim 1 and Jeong Dong Lee For the efficient breeding of black soybean, a core collection is needed using genetic diversity study. In this study we evaluated 470 black soybean accessions with green cotyledon from RDA gene bank, and analyzed their genetic diversity, population structure, and core collection using the SoySNP6K Illumina Infinium BeadChip. Recently SNP markers are broadly used in plant by development of genome complexity reduction sequencing technologies. For data analysis of genetic diversity, gene diversity and polymorphism information content (PIC) were calculated using the genetic analysis package PowerMarker V3.25. Phylogenetic dendrogram was constructed with UPGMA based on genetic distance matrix using the same software. Polymorphic information content (PIC) values ranged from 0.00 to 0.38 with an average of The gene diversity also ranged from 0.00 to 0.50 with an average of A core collection was selected 89 accessions using PowerCore V1.0. For evaluation about coverage of selected core collection, we have analysis data including agronomic traits and functional components. A model based program STRUCTURE was used to study genetic relationship among individual accessions. *Corresponding Author: Tel , dock0409@korea.kr 103

146 PA-115 Jinkwan Jo 1*, Preethi M Purushotham 1, Koeun Han 1, Heung-ryul Lee 2, Gyoungju Nah 3, Byoung-Cheorl Kang Single nucleotide polymorphisms (SNPs) play an important role as molecular markers in plant genomics and breeding studies. Although onion (Allium cepa L.) is an important crop globally, relatively few molecular marker resources have been reported due to its huge genome size with high heterozygosity. Genotyping-by-sequencing (GBS) offers a greater degree of complexity reduction followed by concurrent SNP discovery and genotyping for species with complex genome. In this study, GBS was employed for SNP mining in onion that currently lacks reference genome. The segregating F 2 population derived from across between NW-001 and NW-002 as well as the multiple parental lines (M1, M2 from NW-001 and P1, P2 from NW-002 ) were used for GBS analysis. A total of Gbp raw sequence data were generated and 1,851,428 SNPs were identified from the de novo assembled contigs. Stringent filtering resulted in 10,091 high fidelity SNP markers. Robust SNPs satisfying the segregation ratio and evenly distributed in the mapping population were applied for onion genetic map construction. The final map resulted in eight linkage groups and spanned a genetic length of 1,383 centimorgan (cm) with an average marker interval of 8.08 cm. Moreover, these robust SNPs were further analyzed using high-throughput Fluidigm technology for marker validation. This is the first study in onion that developed genome wide SNPs using GBS. The developed SNP markers and linkage map will be a valuable tool for genetic mapping of important agronomic traits and marker assisted selection in onion breeding programs. *Corresponding Author: Tel , bk54@snu.ac.kr PA-116 Hye Seon Kim, Seok-Jun Moon, Kyong Sil Lee, Duk-Ju Hwang, Il-Pyung Ahn, Shin-Chul Bae, Myung Ok Byun, Sang Ryeol Park * The NPR1 (nonexpresser of pathogenesis-related genes) is key regulator of salicylic acid (SA)-mediated resistance and functionally conserved in diverse plant species, including rice (Oryza sativa L.). The rice genome contains five NPR1-like genes. In this study, one of rice homologous gene, OsNH5 was found to be induced by rice bacterial blight pathogen Xanthomonas oryzae pv. oryzae, and the defense molecules benzothiadiazole (BTH) and salicylic acid (SA). Over-expression of OsXRI5 conferred disease resistance to bacterial blight. OsXRI-overexpressed transgenic lines were shown the higher level expression of several pathogenesis-related protein genes, OsPR4, OsPR10 and OsPAL, than wild type rice. The OsXRI5-green fluorescent protein (GFP) fusion protein was localized in the nucleus. This study provides a practical approach for the improvement of disease resistance in rice. *Corresponding Author: srpark@korea.kr 104

147 PA-117 Daniel Hailegiorgis 1,3, Chong Ae Lee 2, Song Joong Yun 1,2* The endosperm proteins of Ethiopian durum wheat varieties have been isolated and characterized. Sequential extraction method was used to separate individual protein fractions according to their solubility. Prolamins (also known as large monomeric gluten proteins), were identified to be predominant protein fractions (51.47%). Albumins and globulins accounted for only 27% of the total protein. Under reduced condition, polyacrylamide-gel electrophoresis has revealed a wide range of molecular weight (MW) for albumin and globulin proteins. The molecular weight range of globulin was KDa. The electrophoretic pattern of globulin revealed more polymorphisms than that of albumin. High polymorphism, both in terms of band intensity and occurrence, was observed between 15 and 35 KDa. The presence of polymorphism in this region suggests that globulins could also be used as suitable and useful genetic markers to discriminate genotypes. In sum, five globulin dominant genes were identified with sizes of 15, 22, 35, 55, and 70 KDas. The gel electrophoresis result of albumin proteins did not show any significant variation among the genotypes. The number and position of bands were similar for all varieties. When compared to globulins, albumin proteins had wider molecular weight range (10 KDa to 130 KDa). Unlike globulins, there were only two prominent bands in albumins (73 KDa and 9 KDa). The ratio of monomeric to polymeric proteins was close to 4:1. This ratio is among the highest reported so far and it has some implication in quality of processed products. The cutoff point of 100 in the dendrogram that was constructed based on the dissimilarity matrix of the total protein content and individual protein fractions discriminated the 20 durum wheat varieties into 3 major classes. Our results could be useful for plant scientists, bread and pasta making industries in selecting good raw materials with high protein quality for end-users. Acknowledgement: This work was supported by the grant from Regional Subgenebank Support Program of Rural Development Administration, Republic of Korea. *Corresponding Author: sjyun@jbnu.ac.kr PA-118 Jeong-Gwan Ham, Sopheap Yun, Rahmatullah Jan, A-Ra Cho, Jun-Hoi Kim, Xiao-Xuan Du, Kyung-Min Kim * In this study, QTL analysis and physical map construction was performed by using Cheongcheong, Nagdong doubled haploid population for detect yield related flag-leaf angle genes. Investigated flag-leaf angle, photosynthetic efficiency, chlorophyll contents about doubled haploid population, RSM (Response surface methodology) was performed by Minitab 17 to analyze relationship between 3 factors. Genetic map was constructed by using Mapmaker (ver 3.0) and Mapchart (ver 2.2). QTL analysis was performed by using WinQTL cart (ver 2.2). When RSM analysis was performed, yield was increased by decreasing of flag-leaf angle, increasing of photosynthetic efficiency, increasing of chlorophyll contents. QTLs about flag-leaf angle was detected at chromosome 4, 12, QTLs about photosynthetic efficiency was detected at chromosome 3, QTLs about chlorophyll contents was detected at chromosome 11. When constructed physical map based on detected QTLs, 9 genes at qfa4, 2 genes at qfa11, 7 genes at qpe3, 6 genes at qcc11 were detected. As flag-leaf is known to be expressed by differentiation of cell elongation, genes related to cell elongation were detected in this study. This study will be useful for basic data of development and breeding of rice cultivars. *Corresponding Author: Tel , kkm@knu.ac.kr 105

148 PA-119 Jin-Hee Kim, Jun-Hoi Kim, Xiao-Xuan Du, Sopheap Yun, Kyung-Min Kim * According to the Food and Agriculture Organization (FAO) statistics, the world population will increase to about 91 million (Asia 51 million, Africa 19 million). A rise in the world s population means increased need for food. Sweet potato (Ipomoea batatas L.) is one of the alternatives to solving the problem. Thus, in the world breeding practice for improving the quality of sweet potato is ongoing. Recently, development of molecular marker for sweet potato demonstrated good potential to be used in genetic selection. In this study, cdna library were constructed from total RNA. A total of 789 of cdna were cloned in E. coli by employing pgemt-easy vector. As many as 579 EST-SSR markers were designed (73.38%) from the known cdna nucleotide base sequences. Developed EST-SSR marker expected length is range from 100 to 380bp. The motif sequence types of the EST-SSR markers were varied, of which most of the motif sequence types were di- and penta-, respectively, while the most found motifs was CAGAAT(29.0%) and TCT(2.8%). 21 cultivars and Yeseumi X Annobeny populations used for construction of genetic map. molecular analysis on sweet potato could be done more efficiently. Thus, we can develop high quality sweet potato while overcoming the challenge from climate change and unfavorable conditions. *Corresponding Author: Tel , kkm@knu.ac.kr PA-120 Kyu-Chan Shim, Luong Ngoc Ha, Sun Ha Kim, Yun-A Jeon, Hyun-Sook Lee, Xin Lu, Sang-Nag Ahn * In plant, chlorophyll content and leaf senescence is associated with grain filling and yield. We analyzed a QTL controling chlorophyll content using introgression line (CR7501). CR7501 derived from a cross between Oryza sativa Hwaseong as a recurrent parent and wild species O. grandiglumis as a donor parent showed higher chlorophyll content than Hwaseong. For QTL analysis, 58 F 3 and 38 F 4 lines were developed from across between CR7501 and Hwaseong. A QTL for chlorophyll content (qcc2) was detected in chromosome 2 and explained 24.63% of phenotypic variation. To examine the senescence effect of qcc2, dark-induced senescence (DIS) was conducted. Detached leaves from Hwaseong and CR7501 were incubated on 3mM MES buffer (ph 5.8) at 27 under complete dark condition. CR7501 has higher chlorophyll content with delayed senescence than Hwaseong. To know whether the qcc2 has leaf functionality during the DIS, ion leakage test and Fv/Fm measurement were performed. In the Fv/Fm value, significant difference was observed at 6 days after incubation. But, ion leakage rate was not significantly different. These results might suggest that the qcc2 is possibly associated with chlorophyll content and stay-green phenotype. Acknowledgement: This work was supported by the grant from the Next-Generation Biogreen 21 Program for Agriculture & Technology Development (Project No. PJ ). *Corresponding Author: Tel , ahnsn@cnu.ac.kr 106

149 PA-121 안영은 1, 2, 허온숙 1*, 노나영 1, 고호철 1, 전영아 1, 성정숙 1, 이주희 1, 백형진 1, 이준대 풋마름병 (bacterial wilt) 은열대, 아열대, 온대지역에걸쳐전세계에서약 50 과 400 종이상의식물에서병해를일으키며특히가지과작물에큰피해를입히고있다. 이에대한다양한방제방법이제시되고있지만현재풋마름병저항성품종을사용하는것이가장효과적인방법으로알려져있다. 따라서토마토풋마름병저항성유전자원을탐색하기위하여효율적이고정확한풋마름병저항성평가체계를구축하는것이필요하다. 병저항성을평가방법으로는생물검정과마커검정방법이있는데풋마름병은유전적요인은물론온도, 습도와같은환경적요인에도영향을받기쉬우므로생물검정과마커검정을병행하여야정확한데이터를얻을수있다. 따라서본연구에서는토마토풋마름병저항성을정확하게검정할수있는분자표지를개발하고자하였다. 식물재료로는토마토유전자원 61 개와저항성대조구 4 개, 이병성대조구 1 개를사용하였고, 병원균은국립원예특작과학원에서분양받은 Ralstonia solanacearum WR-1 isolate 를사용하였다. 먼저다양한토마토유전자원에대해풋마름병생물검정을실시하여극단적으로저항성과이병성을나타내는각 48 개체를선택하고 GBS 분석을통해저항성과이병성간변이를보이는 SNP 를탐색하였다. 이후 GWAS 분석으로 QTL 을분석한후 QTL 에연관된 SNP 를선발하여 HRM 분자표지로개발하였다. 두개의 QTLs 이염색체 6 번 (Bwr-6) 과 12 번 (Bwr-12) 에서탐색되었는데 12 번에있는것이주동 QTL 이었다. 이주동 QTL 에아주가깝게연관된 10 개의 SNP 를이용하여 9 개의 HRM 분자표지를개발하였고, 개발된분자표지를다양한유전자원에적용해본결과생물검정결과와 100% 일치하는분자표지를 2 개 (BWR12-HRM1 과 BWR12-HRM2) 선발할수있었다. 이과정에서기존에개발되어있던 SSR 분자표지 (SML12-8 과 SML12-9) 를같이실험하였지만 100% 일치하는것은없었다. 본연구에서개발된 2 개의 SNP 분자표지는앞으로토마토유전자원풋마름병저항성검정에활용될수있을것이라고생각되며, 앞으로추가적인연구를통해풋마름병저항성주동유전자를클로닝하는데에기여할수있을것이라고생각된다. * 주저자 : Tel , oshur09@korea.kr PA-122 Kyo-Sang Lee, Hyun A Jang, Sang-Keun Oh * Grapes have cultivated worldwide, specially grown for brewing purposes and accounting for about one-third of the world's fruits production. However, grape anthracnose disease caused by Colletotrichum spp is an economically important disease in grape production. Therefore, we conducted a study to identify the trait associated with grape disease-resistant and performed genome-wide association study(gwas) for pheno/genomics using grape core collections. Firstly, we conducted to prove the pathogenicity of the fungal isolates Colletotrichum acutatum and C. gloeosporioides which were inoculated onto healthy grape leaves by detached method. Results of pathogenicity test from 844 grape cultivars(obtained from RDA Korea) showed 726(87%) susceptible and 118(13%) resistance, respectively. Secondly, we finally selected 350 cultivars (118 resistance and 232 susceptible cultivars) and constructed the Genotyping-by-Sequencing(GBS) library with 96 barcode sets to find disease-resistant related(nb-lrr) genes. After that, the experiment was carried out to select the candidate genes, phenotype data converted into analyzed format data and performed GWAS(Genome-Wide Association Study) analysis with GBS data(using TASSEL software). As a results of GWAS, we identified 6 resistance-related candidate genes for C. gloeosporioides and 7 candidate genes for C. acutatum using filtered 77,126 SNPs by their trait. Among them, only 2 candidate genes were included disease resistance LRR family protein. However, it is necessary to confirm the current results which are actually related or not to resistant genes. Therefore, additional studies should be carried out to confirm resistance genes which are related to current candidate genes. *Corresponding Author: Tel , sangkeun@cnu.ac.kr 107

150 PA-123 Min-Sung Kang 1, Jong Hyang Bae 2, Soon Ju Park Dosage of florigen activation is one of most important yield trait in tomato because it controls tomato shoot growth. Fine-tuning of the dosage effect to shoot growth give a new window for manipulating tomato yield in the field. Here we studies new genetic variants effect in florigen activation complex in tomato, which are capable of using as genetic resources for high yield tomato breeding. We were isolated three new mutant alleles in Self Pruning (SP) locus, known as a tomato anti-florigen, from 200 semi-determinate core collection lines. A single amino acid substitution mutant, sp-337 produced more sympodial shoots per plant with the potential to increase yield through whole life under determinate growth than the one of sp classic. IL of Solanum pennellii producing two leaves on each sympodial shoot was caused by significant increase of SFT expression. IL5-4 carrying S.penSP5G results in late flowering time in S. lycopersicum. Additionally, CR-sp5g, 60bp deletion mutant induced by CRISPR/cas9 system, showed early flowering producing 6 leaves on primary shoot meristem. Altogether, these genetic variations are new resources working on a single florigen pathway for tomato breeding and they could be used as multiple genetic tools to overcome background issue on breeding process. Author duke0357@naver.com PA-124 Hayoung Song and Yoonkang Hur * Molecular markers are useful techniques to shorten the maintenance and management costs of crops through early selection Therefore, it is very important to develop markers that are practically applicable to breeding materials. Cold stress, including freezing, limits crop yield and cultivation area. Freezing tolerance is one of the major horticultural features of cabbage plants. We developed cold - tolerant molecular markers for early selection of freezing-tolerant lines in cabbage cultivation. To develop gene-based molecular markers for freezing-tolerant cabbage, we focused on Circadian Clock Associates 1 (CCA1) and Cold shock domain containing protein 5 (CSDP5). CCA1 is a core circadian clock component that affects metabolic pathways and confers cold tolerance by upregulating CBF pathway genes. CSDP is known to help cells survive at temperatures below optimal growth temperatures. We cloned and analyzed CCA1 (BoCCA1s), and CSDP5 genes (BoCSDP5s) from seven inbred lines and one cultivar of Brassica oleracea ssp. capitata. Two types of alleles were detected: BN106-type (freezing-tolerant; BoCCA1-1, BoCSDP5-106) and BN107-type (freezing-susceptible; BoCCA1-2, BoCSDP5-107). Numerous InDels (insertions/deletions), SSRs (simple sequence repeats), and SNPs (single nucleotide polymorphisms) were found in the exons and introns of BoCCA1s and BoCSDP5s Using InDels and SNPs, we designed PCR primer pairs to distinguish freezing-tolerant lines, which we validated using 102 cabbage lines and cultivars. In the case of allelic variation in CCA1, Inbred lines possessed either the BN106-type or BN107-type allele, but most cultivars had both alleles. Freezing-tolerant cabbage had BN106-type InDels and/or BN106-type SNPs regardless of the presence of BN107-type InDels and SNPs. BN106-type SNPs appear to be more widely detected in freezing-tolerant cabbages than BN106-type InDels. Such markers can best be utilized for cabbage breeding using several markers derived from different genes and independently established inbred lines from different seed companies. Author hayoung282@hanmail.net 108

151 PA-125 Daewoong Lee 1, Tae-Hwan Jun 1* 1 Peanut or groundnut (Arachis hypogaea L.) is a major economic legume crop widely cultivated in tropical and subtropical regions of the world and an important source of protein and vegetable oil especially unsaturated fatty acid (such as oleic acid) for human nutrition. Cultivated peanut is an allotetraploid (AABB; 2n=4x=40) with a relatively large genome size of 2800 Mb/1C, which is presumed to have derived from a single recent hybridization event between two diploid ancestors of A. duranensis (the A genome) and A. ipaensis (the B genome). We resequenced two Korean peanut cultivars K-Ol (Arachis hypogaea ssp. fastigiata L.) and Pungan (Arachis hypogaea ssp. hypogaea L.), which were developed at the Department of Southern Area Crop Science, National Institute of Crop Science (NICS) in Milyang. We identified various types of genetic markers including single nucleotide polymorphisms (SNPs), insertions/deletions (Indels), simple sequence repeats (SSRs), and Cleaved Amplified Polymorphic Sequences (CAPS). To verify the availability of markers found in the study, we screened some of polymorphic markers for 96 peanut varieties that are made up of 5 different origins. Our results are likely to provide a valuable resource for the peanut breeders and researchers. *Corresponding Author: Tel , thjun76@pusan.ac.kr PA-126 Juhun Lee 1*, Min-Young Kang 1, Koeun Han 1, Doyeon Hwang 1 and Byoung-Cheorl Kang 1 1 It is reported that capsaicinoids synthase (CS) is encoded by the Pun1 gene and synthesize pungent causative compound, capsaicinoids. Because of the capsaicinoids, mammals can feel the pungency when they consume the peppers (Capsicum spp.). Until now most of the non-pungent peppers had mutation in the Pun1 gene. We isolated a non-pungent mutant from 1480 M 2 mutant lines, originated from pungent Yuwol-Cho (C. annuum). To figure out the novel locus which is controlling pungency, F 2 population between Yuwol-Cho (C.annuum) and its isolated mutant line was analyzed. The segregation ratio of pungency in F 2 converged on 15:1, which means that duplicate gene action explains this phenomenon. However, the expression levels of BCAT, ACL, KAS, FAT, PAL, Pun1 genes decreased in isolated non-pungent mutant parent. The expression levels of four fatty acid biosynthesis genes in the capsaicinoids biosynthesis pathway showed systematical decreases. This discordance between F 2 segregation ratio and the expression levels analysis by real-time PCR means that certain genes affected the activation of fatty-acid biosynthesis pathway genes. From this research, the novel gene regulating pungency will be elucidated. *Corresponding Author: Tel , bk54@snu.ac.kr 109

152 PA-127 이현숙 1, 심규찬 1, 김선하 1, 송원용 2, 안상낙 1* 1 2 식물의미량원소아연 (Zn), 철 (Fe), 구리 (Cu), 망간 (Mn), 칼슘 (Ca) 등은다량원소와함께식물생육에중요한요소이며철, 아연과같은미량원소는동물의생육, 발달에있어서필수적인원소이다. 본연구에서는벼종자의미량원소을조절하는양적형질유전자좌 (QTLs) 을탐색하였다. 기존의연구에서납저항성으로알려진잡초벼 KH2J 와재배벼밀양 23 호를교잡한 F2 집단을이용하여 QTLs 분석을실시하였다. 벼종자는수확후현미를 65% 질산에녹인후 ICP-MS (inductively coupled plasma mass spectrometry) 로미량원소아연, 철, 구리, 망간, 칼슘, 추가적으로납 (Pb), 카드늄 (Cd), 비소 (As) 의함량을조사하였다. 120 개의 F2 집단을총 92 개의 SSR marker 로유전분석한결과총 8 개의원소에대하여 single point analysis 분석으로총 27 개, CIM (composite interval mapping) 분석으로 22 개의유전자좌를탐지하였고이들중염색체 5 번에아연과철, 망간을조절하는 QTL 이공통적으로탐지되었다. 아연함량조절 QTLs 은모든분석에서공통적으로염색체 5 번이외에 8 번과 10 번에서도탐지되었다. F2 집단에서이들 QTLs 을포함하는계통을선발하여아연함량을비교한결과, 밀양 23 호가 24.5ng/mg, 선발된 CR4046 (CR5113) 가밀양 23 호보다높은 34.7ng/mg 을보였다. 이계통을다음세대로진전시켜밀양 23 호와현미아연함량을비교한결과밀양 23 호가 18.2ng/mg 일때 CR4046 (CR5113) 은 33.7ng/mg 로높은함량을유지하였다. 추가적으로 KH2J 계통이보이는까락과까락색에관여하는 QTLs 이염색체 4 번에서공통적으로탐지되었고부선색에관여하는 QTLs 또한염색체 4 번, 6 번에서탐지되었다. 본연구에서는벼종자의미량원소조절 QTLs 을탐지하였고, 특히아연함량조절이우수한계통을선발하였다. 현재이들아연, 철함량과까락관여유전자좌의고밀도지도작성을진행중에있다. * 주저자 : Tel , ahnsn@cnu.ac.kr PA-128 Daewoong Lee 1, YongSuk Chung 2, Changsoo Kim 2, Tae-Hwan Jun 1* 1 2 A population of 186 F2 individuals derived from a cross of 'Umjinara' x 'Sinsunhwang' and the two parental lines were used for this study. In general, Allium species including onion has very large genome sizes ranging from approximately 10 to 20 Gbp, which have complicated genomic studies and precluded genome sequencing until recently. For the development of framework map, various types of markers including SSRs, RAPD, SNPs, and CAPS makers have been used for polymorphism test. Especially, a lot of SNP and CAPS markers were obtained from the onion transcriptome sequence by RNASEQ of two parental lines. The GBS libraries have been constructed based on a modified protocol from Poland Lab using a two-enzyme system. We have been developing polymorphic markers for two parental lines, and genotyping for all F2 individuals were completed for a number of polymorphic markers. For the construction of GBS libraries, a set of 192 barcoded adapters were generated from complementary oligonucleotides with XhoI overhang sequence and unique barcodes of 4-8 bp length. The optimum conditions were determined for GBS analysis, and sequencing for all 186 F2 lines has been conducted using NGS platform. *Corresponding Author: Tel , thjun76@pusan.ac.kr 110

153 PA-129 Daewoong Lee 1, YangJae Kang 2, Yul-Kyun Ahn 3, Taehwan Jun 1* Bacterial wilt (BW) caused by Ralstonia solanacearum is one of the most common soil-borne vascular diseases of many solanaceous crops such as pepper and tomato. This study aimed to identify QTL for bacterial wilt resistance in a 169 F 8 recombinant inbred line (RIL) population obtained from across of YCM334 x Taean using genotyping by sequencing approach. First, we resequenced YCM344 and Taean that are the parental lines of recombinant inbred lines (RIL). Compared to the known SNPs, we could identify the novel variations only existing in both parental lines and we annotated the informative SNPs by neighboring genes that are already known for disease resistance such as R genes and stress response genes. Moreover, via comparative analysis, we retrieved the SNPs located in the genomic regions that are in synteny with tomato genome. The final genotype matrix from GBS analysis resulted in 2684 SNPs for all 169 individuals, and total 14 linkage groups were constructed while 10 linkage groups represent chromosome 3 to 12, chromosome 1 and 2 were comprised with two linkage groups for each. The genetic map was ranged from 68.8 (chr.11) to (chr.03) cm with the mean length of 89.3 cm, and covered approximately cm with 1820 unique loci (or bins). The average distance between mapped loci was about 0.7 cm with a range from 0.1 to 11.8 cm. We found significant association with five SNP markers and phenotype segregation as QTL with LOD threshold in different five chromosomes based on the high density genetic map. *Corresponding Author: Tel , thjun76@pusan.ac.kr PA-130 Ah-Rim Lee 1, Heng Wang 1, Seong-Gyu Jang 1, Da-Eun Im 1, Soon-Wook Kwon 1* 1 Rice (Oryza sativa L.) is one of major crops and a staple food in Asia, which is cultivated in temperate regions and at high altitudes of tropical regions. Direct-seeding has become increasingly important in rice growing areas because direct seeding in rice can reduce the labor requirement and the labor costs. Low-temperature germinability is an important trait in direct seedling culture, where rice is sown directly. Low temperature condition during sowing stage decrease yields and increase weed competition in rice. Despite of the importance of low-temperature germinability in rice cultivation, the genetic mechanism of this trait is well unknown. In this study, we evaluated the low-temperature germinability of 137 rice accessions and conducted GWAS to identify candidate genes related to low-temperature germinability GWAS analysis performed using the GAPIT with PCA + Kinship model. These results could provide basic information to enable the introduction of genes related to low-temperature germinability and be useful to developing direct-seeding cultivars. *Corresponding Author: Tel , swkwon@pusan.ac.kr 111

154 PA-131 Min Kyoung You 1,2 and Sun-Hwa Ha 1,2* 1 2 Terpene metabolites are composed of isoprene building blocks (C 5), isopentenyl diphosphate (IPP) and dimethyl allyl diphosphate (DMAPP) biosynthesized by a plastidial methylerythritol 4-phosphate (MEP) pathway and a cytosolic mevalonate (MVA) pathway. The isoprene building blocks (C 5 ) are sequentially condensed to generate the major terpenoid precursors, geranyl diphosphates (GPP) for monoterpenoids, farnesyl diphosphates (FPP) for sesquiterpenoids, geranyl geranyl dihphosphates (GGPP) for diterpenoids, and solanesyl diphosphates (SPP). These terpenoid precursors, GPP, FPP, GGPP, and SPP, are biosynthesized by GPS (GPP synthase), FPS (FPP synthase), GGPS (GGPP synthase), and SPS (SPP synthase), and are modified to generate various monoterpenes, sesquitepenes, diterpenes, and ubiquinones through cyclization, reduction, glycosylation, and so on. In this study, 10 predictive rice genes as major prenyltransferase responsible for GPS, FPS, GGPS and SPS are identified using sequence based analyses on in silico databases, and their biochemical activity modules are predicted. Their expression patters on different tissues and developmental stages were investigated using quantitative RT-PCRs, and the subcellular localizations were determined using their GFP-fusion constructions. The analysis of subcellular localization show that the terpenoid precursors are predictively compartmentalized into cytosol, chloroplasts, mitochondria, and golgi apparatus. The predictively compartmentalization analysis could be useful for developing efficient and unique rice terpenoid metabolic engineering, when their biochemical activities would be completely identified. *Corresponding Author: sunhwa@khu.ac.kr PA-132 Chon-Sik Kang 1*, Kyeong-Hoon Kim 2, Young-Keun Cheong 1, Jae-Han Son 1, Jong-Chul Park 1, Yang-Kil Kim 1, Kyong-Ho Kim 1, Tae-Il Park 1, Young-Mi Yoon 1, Ouk-Kyu Han 3, Gi-Heung Hong 4, Jin-Kyeong Choi 5, Jae-Ki Song 6, Jeong-Suk Bae 7, Bo-Kyeong Kim 1, Chulsoo Park Baekkang, a winter wheat(triticum aestivum L.) cultivar was developed by the National Institute of Crop Science, RDA. It was derived from the cross Topdong/Klasic during Baekkang was evaluated as Iksan366 in advance yield trial test in It was tested in the regional yield trial test between 2013 and Baekkang is an awned, semi-dwarf and hard winter wheat, similar to Keumkang and Jokyung (check cultivar). The heading and maturing date of Baekkang were similar to Keumkang and Jokyung. Baekkang had higher test weight (806 g/l) and 1,000-grain weigh (47.5g) than Keumkang (710 g/l and 46.4g, respectively) and Jokyung (805 g/l and 46.1g, respectively). Baekkang showed resistance to Fusarium head blight than Keumkang and Jokyung. Baekkang showed higher protein content (12.4%), SDS-sedimentation volume (60.0ml ) and gluten content (10.1%) to Keumkang (12.1%, 61.3ml and 9.9%, respectively) and Jokyung (11.1%, 49.5ml and 8.3%, respectively). It showed higher lightness (92.40) in flour color than Jokyung (91.75, respectively). Baekkang showed higher volume (950ml ) of baking bread than Keumkang (860ml ) and Jokyung (900ml ). Baekkang exhibited lower firmness (1.96N) of crumb compared to Keumkang (2.54N) and Jokyung (2.14N). Average yield of Baekkang in the regional adaptation yield trial test was 5.05 MT/ha in upland and 5.20 MT/ha in paddy field, which was 10% and 18% higher than those of Keumkang (4.69 MT/ha and 4.45 MT/ha, respectively). *Corresponding Author: Tel , kcs1209@koera.kr 112

155 PA-133 Chon-Sik Kang 1*, Kyeong-Hoon Kim 2, Young-Keun Cheong 1, Jae-Han Son 1, Jong-Chul Park 1, Yang-Kil Kim 1, Kyong-Ho Kim 1, Tae-Il Park 1, Young-Mi Yoon 1, Ouk-Kyu Han 3, Gi-Heung Hong 4, Jin-Kyeong Choi 5, Jae-Ki Song 6, Jeong-Suk Bae 7, Bo-Kyeong Kim 1, Chulsoo Park Saekeumkang, a winter wheat(triticum aestivum L.) cultivar was developed by the National Institute of Crop Science, RDA. It was derived from the cross Keumkang/Olgeuru during Saekeumkang was evaluated as Iksan367 in advance yield trial test in It was tested in the regional yield trial test between 2013 and Saekeumkang is an awned, semi-dwarf and semi-hard winter wheat, similar to Keumkang (check cultivar). The heading and maturing date of Saekeumkang were similar to Keumkang. Saekeumkang had higher test weight (808 g/l) and 1,000-grain weigh (47.0g) than Keumkang (710 g/l and 46.4g, respectively). Saekeumkang showed resistance to winter hardiness and pre-harvest sprouting, which lower pre-harvest sprouting rate (8.1%) than Keumkang (16.5%, respectively). Saekeumkang showed moderate resistance to Fusarium head blight. Saekeumkang showed high milling rate (72.0%), and lower protein content (11.6%), SDS-sedimentation volume (41.8ml ) and gluten content (7.6%) to Keumkang (12.1%, 61.3ml and 9.9%, respectively). It showed higher lightness (93.54) in flour color than Keumkang (91.75, respectively). Saekeumkang showed higher amylose content(24.7%) and peak viscosity (118BU) of to Keumkang (23.7% and 114BU, respectively). Saekeumkang exhibited lower hardness (3.65N), higher springiness (0.92) and higher cohesiveness (0.67) of cooked noodles compared to Keumkang (4.13N, 0.91 and 0.62). Average yield of Saekeumkang in the regional adaptation yield trial test was 5.59 MT/ha in upland and 5.66 MT/ha in paddy field, which was 20% and 28% higher than those of Keumkang (4.69 MT/ha and 4.45 MT/ha, respectively). *Corresponding Author: Tel , kcs1209@koera.kr PA-134 Jae-Young Song 1, Seon-Kyeong Song 1, Dal-A Yu 1, Me-Sun Kim 1, Sothea Ouk 1, Kwon Kyoo Kang 2, Yong-Gu Cho Rice (Oryza sativa) cultivars show impairment of growth in response to abiotic stresses such as drought, salinity, heat and cold at the early seedling stage. It is important to precisely screen valuable genetic resources for improving stress tolerance and understanding tolerance mechanism to abiotic stresses. Because there are differences of experiment designs for screening of tolerant plant in several studies related to abiotic stress, this study has performed to provide the rapid and efficiency screening method for selection of tolerance rice to drought and salinity stresses. Two week-old rice seedlings that reached about three leaf stage were treated with drought and salinity stresses and examined tolerant levels with tolerant and susceptible control varieties, and transgenic plants. To determine the optimum concentration for the selection of drought and salinity condition, tolerant, susceptible and wild-type plants were grown under three soil moisture contents (5, 10 and 20% water contents) and three NaCl concentrations (100, 200 and 250 mm) for 10 days at seedling stage. 200 mm NaCl concentration and 5% moisture content soil were determined as the optimum conditions, respectively. The described methodologies in this study are simple and efficiency, and it might valuable for the selection of drought and salinity stress tolerance in plants at the 3,4-leaf-seedling stage. Acknowledgement: This work was supported by a grant from the Next-Generation BioGreen 21 Program (PJ ), RDA, Republic of Korea. Corresponding Author: ygcho@cbnu.ac.kr 113

156 PA-135 Chon-Sik Kang 1*, Kyeong-Hoon Kim 2, Young-Keun Cheong 1, Jae-Han Son 1, Jong-Chul Park 1, Yang-Kil Kim 1, Kyong-Ho Kim 1, Tae-Il Park 1, Young-Mi Yoon 1, Ouk-Kyu Han 3, Gi-Heung Hong 4, Jin-Kyeong Choi 5, Jae-Ki Song 6, Jeong-Suk Bae 7, Bo-Kyeong Kim 1, Chulsoo Park Johan, a winter wheat(triticum aestivum L.) cultivar was developed by the National Institute of Crop Science, RDA. It was derived from the cross 96PYT115/Suwon262//Joeun during Johan was evaluated as Iksan372 in advance yield trial test in It was tested in the regional yield trial test between 2014 and Johan is an awned, semi-dwarf and hard winter wheat, similar to Keumkang (check cultivar). The heading and maturing date of Johan were earlier to Keumkang. Johan had lower test weight (806 g/l) and 1,000-grain weigh (38.7g) than Keumkang (815 g/l and 46.5g, respectively). Johan showed resistance to winter hardiness and pre-harvest sprouting, which lower withering rate on the high ridge (4.8%) than Keumkang (32.6 %, respectively). Johan showed lower protein content (10.0%), SDS-sedimentation volume (41.7ml ) and gluten content (7.3%) to Keumkang (11.9%, 59.5ml and 9.9%, respectively). It showed higher lightness (92.18) in flour color than Keumkang (91.86, respectively). Johan showed higher lightness (83.75) of noodle dough sheet than Keumkang (83.00). Johan exhibited similar hardness (4.25N) and higher springiness and cohesiveness of cooked noodles (0.90 and 0.63) compared to Keumkang (4.35N, 0.89, and 0.60, respectively). Average yield of Johan in the regional adaptation yield trial test was 5.56 MT/ha in upland and 5.17 MT/ha in paddy field, which was 24% and 22% higher than those of Keumkang (4.47 MT/ha and 4.22 MT/ha, respectively). *Corresponding Author: Tel , kcs1209@koera.kr PA-136 Joonki Kim 1, Hye-Jung Lee 1, Wricha Tyagi 2, Michael Kovach 2, Megan Sweeney 2, Susan McCouch 2*, Yong-Gu Cho 1* 1 2 A deletion analysis of the Oryza sativa dihydroflavonol reductase (DFR) promoter defined a 25 bp region (-386 to -362) sufficient to confer pericarp-specific expression of a β-glucuronidase (GUS) reporter gene in transgenic rice. Site-specific mutagenesis of these conserved sequences and subsequent expression analysis in calli which transiently expressed the mutated promoter::gus gene showed that both bhlh (-386 to -381) and Myb (-368 to -362) binding sites in the DEL3 (-440 to 70) promoter were necessary for complete expression of the GUS gene including the tissue-specific expression of DFR::GUS gene. The GUS gene was expressed well in the mutated Myb (-368 to -362) binding site, but not as strong as in normal condition, implying that the Myb is also necessary to express GUS gene fully. Also, we found the non-epistatic relation between Rc and DFR. There were no changes of expression patterns GUS under the Rc and rc genotypes. Thus, DFR expression might be independent of the presence of functional Rc gene and suggested that Rc and Rd (DFR) share the same pathway controlling the regulation of flavonoid synthesis but not a direct positive transcriptional regulator of DFR gene. These authors contributed equally to this work. Acknowledgement: This research was supported by the OECD Co-operative Research Programme, Fellowship 2010: Biological Resource Management for the Sustainable Agricultural System and from the National Research Foundation program (2014R1A 2A1A ) funded by the Ministry of Science, ICT and Future Planning, Republic of Korea. *Corresponding Author: ygcho@cbnu.ac.kr 114

157 PA-137 Chon-Sik Kang 1*, Kyeong-Hoon Kim 2, Young-Keun Cheong 1, Jae-Han Son 1, Jong-Chul Park 1, Yang-Kil Kim 1, Kyong-Ho Kim 1, Tae-Il Park 1, Young-Mi Yoon 1, Ouk-Kyu Han 3, Gi-Heung Hong 4, Jin-Kyeong Choi 5, Jae-Ki Song 6, Jeong-Suk Bae 7, Bo-Kyeong Kim 1, Chulsoo Park Taejoong, a winter wheat(triticum aestivum L.) cultivar was developed by the National Institute of Crop Science, RDA. It was derived from the cross Xian83(104).11/Keumkang during Taejoong was evaluated as Iksan370 in advance yield trial test in It was tested in the regional yield trial test between 2014 and Taejoong is an awned and hard winter wheat, similar to Keumkang (check cultivar). The heading and maturing date of Taejoong were later to Keumkang. Taejoong had lower test weight (757 g/l) and higher 1,000-grain weigh (48.3g) than Keumkang (812 g/l and 46.1g, respectively). Taejoong showed similar resistance to winter hardiness and pre-harvest sprouting and higher resistance to Fusarium head blight than Keumkang. Taejoong showed lower protein content (11.1%), SDS-sedimentation volume (34.2ml ) and gluten content (8.8%) to Keumkang (11.9%, 59.5ml and 9.9%, respectively). It showed similar lightness (91.90) in flour color to Keumkang (91.86, respectively). Taejoong showed similar lightness (83.02) of noodle dough sheet than Keumkang (83.00). Taejoong exhibited similar hardness (4.24N), springiness and cohesiveness of cooked noodles (0.89 and 0.61) to Keumkang (4.35N, 0.89, and 0.60, respectively). Average yield of Taejoong in the regional adaptation yield trial test was 5.29 MT/ha in upland and 4.61 MT/ha in paddy field, which was 21% and 13% higher than those of Keumkang (4.36 MT/ha and 4.09 MT/ha, respectively). *Corresponding Author: Tel , kcs1209@koera.kr PA-138 Yun-Shil Gho 1*, Sun-A Park 1*, Sung-Ruyl Kim 1,2*, Anil Kumar Nalini Chandran 1, Gynheung An 1 and Ki-Hong Jung 1* 1 2 Peroxiredoxins (Prxs) have recently been identified as plant antioxidants. However, the biological functions in rice, a model crop plant, have not been well studied. In this study, we carried out a comparative transcriptome analysis of 10 rice and 10 Arabidopsis Prxs. As a result, Our phylogenetic analysis revealed that one subgroup contains three rice and three Arabidopsis Type-II PRXs that are expressed ubiquitously. This suggests that they are involved in housekeeping functions to process reactive oxygen species (ROS). A global expression analysis of the PRX family in rice identified tandem duplicates, Os1-CysPrxA and Os1-CysPrxB, in the 1-CysPrx subgroup that are differentially expressed in developing seeds and germinating seeds. Analysis of the cis-acting regulatory elements (CREs) revealed unique CREs responsible for embryo and root or endosperm-preferential expression. In addition, the presence of leaf/shoot-preferential Prxs in rice suggests that they are required in that crop because those plants must tolerate a higher light intensity in their normal growth environment when compared with Arabidopsis Prxs. Downregulation of two Prxs in the 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) mutant caused an albino phenotype, implying that those genes have roles in processing ROS produced during photosynthesis. Network analysis of four Prxs allowed us to model regulatory pathways that explain the underlying protein interaction network. Taken together, a systematic expression analysis of rice Prx family presented in this study will be a useful indicator for further functional studies of rice Prxs in morphological or physiological aspect. *Correspondence: khjung2010@khu.ac.kr 115

158 PA-139 Won Yong Jung 1,, Areum Lee 1,2,, Youn-Sung Kim 3*, Hye Sun Cho 1,2* Chinese cabbage (Brassica rapa ssp.pekinensis) is a typical species which requires vernalization to flowering. Flowering time is an economically important characteristic of Chinese cabbage crop with high leafy head yields and lateness of flowering is a favorable trait. However, there have been few reports on the flowering time-related genes and the flowering mechanism in Chinese cabbage. Here, we conducted genome-wide transcriptome analysis using an inbred line of Chinese cabbage, 4004, which showed the characteristics of early flowering in response to vernalization. In total, 1,677 differentially expressed genes (DEGs) were identified between with and without vernalization. From the transcriptome analysis result, 229 homologs of Arabidopsis flowering-time genes were identified in Chinese cabbage and 56 of these genes were responded to vernalization. qpcr analysis of major flowering genes showed that majority of flowering enhancers were up-regulated in response to vernalization, whereas most of flowering repressors were down-regulated in the condition. Among the major flowering genes, BrFT, BrSOC1/2, BrFLC1/2/3, and BrMAF gene expression were greatly affected by vernalization. Comparative analysis of flowering gene expression between Chinese cabbage and radish showed that expression of GA pathway-related genes was notably different: BrGAI and BrGID1A were expressed in reverse to that in radish. In addition, a number of major flowering genes of radish such as RsFT, RsFRI, RsAP1, and RsLFY, were not expressed, whereas, most of major flowering genes were expressed well in Chinese cabbage. These results suggest that different flowering mechanism may act between Chinese cabbage and radish under vernalization. *Corresponding Author: Tel , hscho@kribb.re.kr PA-140 Marjohn Nino 1, Franz M. Nogoy 1, Me-Sun Kim 1, Jae-Young Song 1, Kwon-Kyoo Kang 2, Yong-Gu Cho 1* 1 2 High throughput transcriptome investigations of immunity in plants highlight the complexity of gene networks leading to incompatible interaction. To identify genes crucial to resistance against Xanthomonas oryzae pv oryzae, functional genetic analysis of selected differentially expressed genes from our microarray data set was carried out. A total of 13 overexpression vector constructs were made using 35S CaMV promoter which drive constitutive expression in rice. Most of the genes are developmentally expressed especially during maximum tillering stage and are commonly highly expressed in the leaves. When screened against Xoo strain K2, the transgenic plants displayed shorter lesion length compared with wild type Dongjin which indicates partial resistance. The levels of ROS continuously magnified after inoculation which indicates robust cellular sensing necessary to initiate cell death. Elevated transcripts levels of several defense-related genes at the downstream of defense signal network also corroborate the phenotype reaction of the transgenic plants. Moreover, expression assays revealed regulation of these genes by cross-communicating signal-transductions pathways mediated by salicylic and jasmonic acid. These collective findings revealed the key immune signaling conduits critical to mount full defense against Xoo. Acknowledgement: This research was supported by a grant from the National Research Foundation (NRF) programs (2014R1A2A1A ) funded by the Korean Ministry of Science, ICT and Future Planning, Republic of Korea. *Corresponding Author: ygcho@cbnu.ac.kr 116

159 PA-141 Keumsun Kim 1,2, Sewon Oh 1,2, Hyeondae Han 1,2, Kidong Hwang 1,2, Min-Gi Lee 1,2, Suji Ahn 1,2, Jiyeon Kim 1,2, Yoon-Kyeong Kim 3, Daeil Kim 1,2* Estimation of genetic diversity among Pyrus spp. is often very difficult owing to the low morphological diversity, lack of differentiating characters among species, and widespread crossability. In present study, we conducted genotyping-by-sequencing technology to estimate the genetically diverse collection of 231 pear accessions and detected 10,186 SNPs. Phylogenetic tree was constructed using MEGA6 with neighbor-joining method and population structure was estimated by using STRUCTURE v based on the set of 10,186 SNPs. Analysis of phylogenetic tree and genetic structure substantiate the identification of tree distinct subpopulations. The first subpopulation contains mainly P. communis, 4 primary pears (P. glabra, P. amygdaliformis, P. nivallis, and P. elaeagrifolia), and hybrids between P. pyrifolia and P. communis. The second subpopulation includes Japanese pears (P. pyrifolia) and the third subpopulation comprises Chinese pears (P. bretschneideri, P. ussuriensis, and P. betulifolia) and Korean native pears. Genetic analysis within each subpopulation revealed patterns of diversity associated with geographical origin. Finally, our findings could be useful for developing a core collection of pear genetic data. *Corresponding Author: Tel , dkpomo@cbnu.ac.kr PA-142 Marjohn Nino 1, Joon ki Kim 1, Franz M. Nogoy 1, Dal-A Yu 1, Sothea Ouk 1, Jae-Young Song 1, Kwon-Kyoo Kang 2, Yong-Gu Cho 1* 1 2 Bacterial blight, caused by Xanthomonas oryzae pv. oryzae, is an emerging disease of rice in Korea causing damage to rice quality and yield. To understand the transcriptional gene network that is crucial to resistance against Xoo, a whole-genome oligonucleotide microarray of Dongjin, a japonica rice which is susceptible to BB, and Jinbaek, which is resistant cultivar were used to infer transcripts of inducible genes between compatible and incompatible interactions at 48 hour post inoculation. Comparison of expression profiles between two cultivars revealed a complex and prominent gene network involved in resistance against bacterial blight. A total of 1,721 differentially expressed genes, designated as K2-induced differentially expressed genes (K2-DEGs), were identified. A large number of inducible genes are more evident in the resistant cultivar, which is threefold higher than in susceptible plant. Functional annotations revealed at least ten categories that are common in both interactions, of which signaling is most abundant, followed by transcription, metabolism, defense, and transport among others. Extensive upregulation of these genes presumably signifies that gene regulatory network leading to incompatible interaction is orchestrated by defense signals and robust cellular reprogramming. Most of the DEGs were assigned in cytoplasm and nucleus, corroborating the idea that critical resistant gene components may be targeted to the nucleus to trigger full immunity response to pathogen. Acknowledgement: This research was supported by a grant from the National Research Foundation (NRF) programs (2014R1A2A1A ) funded by the Korean Ministry of Science, ICT and Future Planning, Republic of Korea. *Corresponding Author: ygcho@cbnu.ac.kr 117

160 PA-143 Keumsun Kim 1,2, Kidong Hwang 1,2, Sewon Oh 1,2, Hyeondae Han 1,2, Min-Gi Lee 1,2, Suji Ahn 1,2, Jiyeon Kim 1,2, Yoon-Kyeong Kim 3, Daeil Kim 1,2* Genotyping-by-sequencing (GBS) is a cost effective and straightforward technology for discovering single nucleotide polymorphisms (SNPs). However, the most important problem is the limit of fragment size to read and this phenomenon can influence on reliability of GBS. In this study, to evaluate the reliability of GBS, we analyzed genetic relationship among Asian pears (Pyrus spp.) using standard set of simple sequence repeats (SSRs) and SNPs derived from GBS, respectively. The genetic diversity was analyzed in 45 pear accessions (2 primary pears, 42 Asian pears, and 1 reference pear). Phylogenetic tree using SSRs was classified into a total of 5 groups. Group I consisted of 24 P. pyrifolia accessions except for Hori. Group II, III, and IV consisted of accessions belonged to P. bretschneideri and P. ussuriensis except for Japanese Golden Russet (JGR) Mikado Decline Resist. (MDR), Yakumo, and Yandangseolri. Group V contained P. fauriei, Bartlett, and other cultivars that consisted of Kiyomaro, Ooharabeni, and Taiheiyo. These cultivars are accessions of interspecific cross between Asian pear and P. communis. This group was out-grouped and served as a reference to determine the relationship among Asian pear accessions. Even though phylogenetic tree using SNPs was divided into 3 groups, it was similar to using SSRs. The only difference is that group II, III, and IV drawn from SSRs were combined into group II in the phylogenetic tree using SNPs. Our study suggests that GBS is reliable and this results may be used for the management and development of pear germplasm collections *Corresponding Author: Tel , dkpomo@cbnu.ac.kr PA-144 Sewon Oh 1,2, Keumsun Kim 1,2, Hyeondae Han 1,2, Kidong Hwang 1,2, Min-Gi Lee 1,2, Suji Ahn 1,2, Jiyeon Kim 1,2, Mockhee Lee 3 and Daeil Kim 1,2* The Actinidia arguta is wild genetic resource of hardy kiwifruit in Korea. In order to provide useful information to germplasm collection, the genetic relatedness and population structure were investigated within A. arguta and other Actinidia accessions (A. polygama, A. macrosperma, A. rufa, A. chinensis, and A. deliciosa). Those other Actinidia accessions were used as references. The phylogenetic relationship and population structure were obtained using single nucleotide polymorphisms derived from genotyping-by-sequencing. Relationship between Actinidia accessions was revealed by neighbor-joining method. In phylogenetic tree, the outgroup contains A. polygama, A. macrosperma, A. rufa, Hort16A (A. chinensis), and Hayward (A. deliciosa). The A. arguta accessions are classified into major two groups. In addition, three clusters were observed in population structure. The maximum delta K was detected at K = 3, which indicates the most likely number of genetic clusters. Cluster I and II are composed of A. arguta accessions and Cluster III is consisted of outgroup species in phylogenetic tree. Therefore, the result of phylogenetic analysis is consistent with population structure. These results could provide an information to develop a core germplasm set for A. arguta. Moreover, additional data such as agronomical traits may be need to classify the A. arguta accessions in detail. *Corresponding Author: Tel , dkpomo@cbnu.ac.kr 118

161 PA-145 Sewon Oh 1,2, Keumsun Kim 1,2, Hyeondae Han 1,2, Kidong Hwang 1,2, Min-Gi Lee 1,2, Suji Ahn 1,2, Jiyeon Kim 1,2, Mockhee Lee 3 and Daeil Kim 1,2* In kiwifruits, the classification of Actinidia chinensis and A. deliciosa is difficult. Morphologically, the two species are very similar and have close phylogenetic relationship. To classify those two species, single nucleotide polymorphisms (SNPs) derived from genotyping-by-sequencing (GBS) were used for constructing phylogenetic tree and population structure in 50 kiwifruits. In the phylogenetic tree, the kiwifruit accessions are divided into 2 groups. The 22 A. chinensis, 18 A. deliciosa, and 3 hybrids are belonged in first group and classified into two subgroups. Subgroup I is consisted of A. chinensis accessions and 2 hybrids, Jacy Green and Mega Gold (A. chinensis A. deliciosa). Subgroup II is composed of A. deliciosa accessions and Bangwoori (A. arguta A. deliciosa). The second group as an outgroup is consisted of A. polygama ( S8 ), A. arguta var. purpurea ( S3 ), A. arguta ( Chiak, K5-1-8, K5-2-3, K ), and A. arguta A. deliciosa ( Skinny Green ). In addition, population structure analysis showed that identified K = 3, which means the kiwifruit accessions are divided into three clusters. Our results suggest that the GBS derived SNPs could distinguish A. chinensis and A. deliciosa. *Corresponding Author: Tel , dkpomo@cbnu.ac.kr PA-146 Jae Yoon Kim 1*, Chang-Ho Kim 1, Yong Weon Seo Gibberellic acids (GAs) are influential plant hormones that function during a plant s whole lifetime. GAs regulate major growth and developmental processes such as seed germination, stem elongation, flowering, and fruit development. Among numerous GA-responsive genes, the GAST family are known to be important growth inducers and regulators of development that act in response to GA. In this study, four GA-stimulated genes were isolated from wheat and designated TaGAST1, 2, 3, and 4 (Triticum aestivum gibberellic acid stimulated transcript 1, 2, 3 and 4).All TaGAST family members encode approximately 100 amino acid residues and include highly conserved cysteine-rich domains termed GASA domains in their C-terminal regions, along with divergent intermediate N-terminal regions. The expression of the TaGASTs was analyzed at the inflorescence development stage, in different tissues, and under the application of phytohormones. TaGAST1 was prominently expressed at the inflorescence development stage in response to phytohormone treatment. However, TaGAST1 was not expressed in the seedlings except under abscisic acid (ABA) treatment. TaGAST2 and TaGAST3 showed moderate expression in the spike but vigorous transcript accumulation in the seedling. TaGAST4 was predominantly expressed only in the seedling. To identify putative interacting proteins of the TaGAST genes during spike development, a yeast two-hybrid assay was conducted and wheat cyclophilin A-1 (TaCypA1) was identified as a TaGAST1 interacting protein. The expression pattern of TaCypA1 in the developing spike was opposite to that of TaGAST1. A bimolecular fluorescence complementation assay revealed that the interaction of TaGAST1 with TaCypA1 is targeted to the plasma membrane. Acknowledgement: This work was also supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (NRF-2017R1A1A1A ) *Corresponding Author: Tel , jaeyoonkim@kongju.ac.kr 119

162 PA-147 Hyeondae Han 1,2, Keumsun Kim 1,2, Sewon Oh 1,2, Kidong Hwang 1,2, Min-Gi Lee 1,2, Suji Ahn 1,2, Jiyeon Kim 1,2, Sunheum Cho 1, Daeil Kim 1,2* 1 2 This study was performed to evaluate genetic diversity between various apple (Malus spp.) accessions using single nucleotide polymorphisms which derived from genotyping-by-sequencing. The genetic relationship among 73 apple accessions was evaluated using a phylogenetic tree, which clustered all the accessions into four groups. Group I is contained three major subgroups with the following characteristics. Subgroup I consisted of wild species (M. prunifolia, M. sieboldii, and others) and both subgroup II and III are composed of crab apples and M. domestica accessions. Group II is characterized by the apple cultivars bred in Korea and their parents accessions. Group III is composed M. domestica accessions which are introduced cultivars and Korean bred cultivar of Seokwang. Group IV consisted of wild species (M. floribunda, M. asiatica, and others), Mollie s Delicious, and Honey Crispy. These results indicate that the 73 apple accessions are mainly divided into wild species and cultivated species. Moreover, the estimated clusters revealed by the population structure showed an optimum value of 3 (K=3), that represented the 73 apple accessions could be categorized into three clusters. Our results are useful for consideration of parental selection in apple breeding. *Corresponding Author: Tel , dkpomo@cbnu.ac.kr PA-148 Hyeondae Han 1,2, Keumsun Kim 1,2, Sewon Oh 1,2, Min-Gi Lee 1,2, Kidong Hwang 1,2, Jiyeon Kim 1,2, Suji Ahn 1,2, Sunheum Cho 1, Daeil Kim 1,2* 1 2 Cleaved amplified polymorphic sequence (CAPS) markers are techniques for identifying polymorphisms at a particular locus that is amplified by polymerase chain reaction (PCR), followed by digesting with restriction enzymes. The purpose of this research was to develop a new cost effective PCR based CAPS marker set using the high-throughput single nucleotide polymorphism (SNP) genotypes developed by the genotyping-by-sequencing (GBS) to distinguish the apple sports. SNP survey was comprised of using 18 apple sports genotypes ( Fuji, 13 sports of Fuji, Hongro, and 3 sports of Hongro ) via GBS that resulted in 11,136 polymorphic SNP markers. After filtering out the polymorphic SNPs using recognition sequences of the 8 restriction endonucleases as a query, 79 SNPs were randomly selected. PCR primers were designed to amplify 450 to 550 bp sequences of each locus and their polymorphisms were visualized on 2% agarose gel. The genetic diversity in sports of Fuji and Hongro was very low, only Sobeak No.3 (sports of Fuji ) and Sobeak No.2 (sports of Hongro ) were distinguished by using CAPS markers. In this study, we succeeded in converting SNP sites into CAPS markers and distinguishing 2 sports of apple accessions. It is anticipated that these putative SNPs could be applied in apple genetic research and breeding. *Corresponding Author: Tel , dkpomo@cbnu.ac.kr 120

163 PA-149 Yeo Jin Lee 1,2, Min Kyoung You 1,2, and Sun-Hwa Ha 1,2* 1 2 In plants, terpenoid precursors are produced through two independent pathways, the cytosolic mevalonate (MVA) pathway and the plastidial methylerythritol 4-phosphate (MEP) pathway. Isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), which are precursor for the supply of terpenoid catalyzed by six enzymes of the MEP pathway. 4-hydroxy-3-methylbut-2-enyl diphosphate reductases (HDR or IspH) is essential enzyme in the last step of MEP pathway. Two IspH genes have been found in Oryza sativa. To profiling of expression patterns, transcript level of OsIspH genes were analyzed among developmental stages and tissues. OsIspH1 is constitutively expressed higher in the leaves, whereas OsIspH2 is expressed in the roots. OsIspH1 complemented that functionally an E. coli IspH deficient mutant (DLYT1) in enzyme activity. OsIspH1 fused sgfp protein was detected only into chloroplasts. Knock-out mutant of OsIspH1 by T-DNA insertional mutagenesis in rice (osisph1) had albino phenotype, and signified that OsIspH1 is critical functions for provide plastidial IPP/DMAPP in the leaves. Also, the osisph1 were observed to be altered in roots of it, in which the number of roots, total weight, and length. In addition, transcript level of OsIspH2 was detected to increase in roots of osisph1 homozygous mutant than wild type plants, but not detected in shoots. These root phenotypes might be concern with transcript level of OsIspH2 increased in roots of osisph1 mutant. We conclude that OsIspH1 and OsIspH2 has tissue specific roles in terpenoids metabolism of shoots and roots, respectively. : equally first authors *Corresponding Author: sunhwa@khu.ac.kr PA-150 양환래, 박선경, 이상희, 김종보 * 알스트로메리아 (Alstroemeria) 는세계 10 대절화에속하는중요한절화작물중하나이다. 알스트로메리아는화색과화형이다양하고절화수명이비교적길며특히, 저온적응성이높아고소득화훼작물로서생산자들에게인기가많아국내시장에서시장규모가증가하는추세이다. 그러나낮은증식률과장기간의번식기간그리고바이러스감염율이높기때문에우량품종의대량증식이어려운현실이다. 따라서기존의근경 (rhizome) 을이용한증식시스템을개선하고또한다른절편체인엽병이나배발생캘러스그리고체세포를이용한새로운대량증식시스템을확립하는과정이향후필요하다. 본연구진은알스트로메리아의근경을이용하여조직배양시 cytokinin 과 auxin 을조합하여기내식물체성장에효과적인호르몬농도조합을선정하기위해본연구를수행하였다. 실험에사용된알스트로메리아는국내육성계통 D187 이고이계통의근경을절편체로사용하였는데, 이근경은 sucrose 30 g L - ¹, gelrite 0.25 %, BA(0.0, 0.5, 및 1.0) mg L - ¹ 와 NAA(0.0, 0.10 및 0.20) mg L - ¹ 가혼용첨가된 MS 배지에치상하여 23±1 C 그리고 16 시간명조건하에서 4 주간격으로계대배양하였다. 2 주마다데이터수집을수행하여총 10 주간측정하였으며, 생체중, 신초길이, 뿌리길이, 뿌리개수그리고 rhizome 개수를측정하였다. 그결과호르몬무처리구에서는신초발생개수가 2 개미만이었지만호르몬처리구에서는신초개수가전부 2 개이상발생하였다. 특히 BA 0.5 mg L - ¹ 와 NAA 0.20 mg L - ¹ 혼용처리를하였을때뿌리개수가 2.23 개로가장많이발생하였고, 생체중증가량또한 3.37g 으로가장높게측정되었으며, 알스트로메리아생장에필요한근경발생개수가 2.64 개로 5 개의호르몬조합중에가장많았다. 이러한결과를적용하면향후국산우량알스트로메리아품종대량생산시스템구축에기여하여우량품종의고효율의증식및농가보급에기여할것으로기대된다. * 주저자 : Tel , jbhee1011@kku.ac.kr 121

164 PA-151 Heebak Choi 1*, Sun-Hwa Ha 1 1 In plants, the terpene synthesis pathway generates more than 30,000 types of second metabolites including phytohormones (GA, ABA, cytokinin and strigolactone) and beneficial products. Interests for terpene synthesis pathway are increasing, but a sophisticated control of terpene synthesis seems difficult to achieve by direct modification of functional enzyme due to its severe defects. We purposed the blue light signal as appropriate condition for screening transcription factors and found ERF group VII family as negative regulator of terpene synthesis pathway. In here, prediction of relationship between ERF group VII family and terpene synthesis were presented. In addition, the characteristic of ERF group VII and comparison of its cis-elements with differential expression patterns were analysed. *Corresponding Author: Tel , backy28@khu.ac.kr PA-152 Wei Tong 1, Qiang He 1, Yong-Jin Park 1,2 1 Mitochondrial genome variations have been detected despite the overall conservation of this gene content, which has been valuable for plant population genetics and evolutionary studies. Here, we describe mitochondrial variation architecture and our performance of a phylogenetic dissection of Korean landrace and weedy rice. A total of 4,717 variations across the mitochondrial genome were identified adjunct with 10 wild rice. Genetic diversity assessment revealed that wild rice has higher nucleotide diversity than landrace and/or weedy, and landrace rice has higher diversity than weedy rice. Genetic distance was suggestive of a high level of breeding between landrace and weedy rice, and the landrace showing a closer association with wild rice than weedy rice. Population structure and principal component analyses showed no obvious difference in the genetic backgrounds of landrace and weedy rice in mitochondrial genome level. Phylogenetic, population split, and haplotype network evaluations were suggestive of independent origins of the indica and japonica varieties. The origin of weedy rice is supposed to be more likely from cultivated rice rather than from wild rice in mitochondrial genome level. This work has been published in Scientific Reports (2017). Corresponding Author: Tel , yjpark@kongju.ac.kr 122

165 PA-153 Kyu-Won Kim 1, Soon-Chun Jeong 2, Ji-Hye Hwang 3, Hye-Won Chang 4, Hee-Jong Koh 4, Jung-Kyung Moon 3, Yong-Jin Park 1, As genetic information for a large amount of crops can be collected rapidly via the next-generation sequencing (NGS) method or DNA chip, population genetics, which was previously described theoretically, has been applied to the field of crop breeding in recent years and its importance has been highlighted. In addition, crop gene mapping techniques based on statistical analysis methods such as genome-wide association study (GWAS) and genomic selection (GS) are emerging as important analytical methods. In order to keep the research level for the national crop breeding program global, a deeper understanding of statistical breeding is needed. We invited world-renowned academics in the field of statistical breeding and held lectures on statistical and bioinformatics tools to organically link genomic information with molecular breeding. More than 50 graduate students from all over the country participated in the study to gain a basic understanding of the various statistical models used in crop breeding and population genetics. Through this training, we hope that breeding research based on population genetics and statistical models will be promoted and thus contribute to cultivating new varieties. Corresponding Author: Tel , yjpark@kongju.ac.kr PA-154 Kyu-Won Kim 1, Won-Hee La 2, Il-Pyung Ahn 3, Yong-Jin Park 1, Rice blast is a serious disease that causes a decrease in rice yield. In Korea, it has implemented a considerable blast resistant cultivation system, but the epidemic of blast can occur at any time. In order to cope with rapidly evolving strains of blast disease, it is necessary to maintain sufficient resistance gene pool and to secure new resistance genetic resources continuously. We identified candidate genes for blast resistance in domestic rice populations. Using various evidences of the blast resistance genes such as sequence homology, transcriptional and genomic association, we summarized those evidences to narrow the defense candidate genes. Corresponding Author: Tel , yjpark@kongju.ac.kr 123

166 PA-155 Kyu-Won Kim 1, Jung-Rye Nam 1, Yong-Jin Park 1,2 1 2 Orthologs are divided into one-to-one orthologs, with one copy of orthologous gene per species, and many-to-many orthologs with multiple copies of orthologous genes per species. Many-to-many orthologs (n: n orthologs) account for a considerable proportion of all types of orthologs. Although, there is no model for comparative analysis from many-to-many orthologs, many studies have used only one-to-one orthologs. If we can make it that many-to-many orthologs are available, then we can compare the function of genes that have not previously been compared. We have developed an algorithm to enable comparative genomic analysis from many-to-many orthologs, by converting many-to-many orthologs to one-to-one orthologs. The algorithm we have developed is applied to monocotyledonous and dicotyledonous plants. A number of many-to-many orthologs were identified and converted to be compared with their functions. The results of this study are expected to contribute to the identification of new gene functions, by analyzing gene function of crops. Corresponding Author: Tel , yjpark@kongju.ac.kr PA-156 Kyu-Won Kim 1*, Jung-Rye Nam 1*, Yong-Jin Park 1,2 1 2 In order to effectively utilize the results of genome-wide association study (GWAS) to the researches for the fields of molecular biology, haplotype, well-made database which is suitable for genetic functional study is needed. Indel has a higher rate of mutation than SNP, and it has a significant effect on gene function changes through frame shift as well as SNP. Indel information is therefore of considerable importance in studies of gene function. However, it is difficult for researchers to easily and freely access Indel information. We constructed haplotype and indel information for rice as a model crop. We expect that the use of indel information will be activated through our study. *These authors contributed equally to this work. Corresponding Author: Tel , yjpark@kongju.ac.kr 124

167 PA-157 Jung-Rye Nam 1*, Kyu-Won Kim 1*, Yong-Jin Park 1,2 1 2 RNA-Seq is a technique for analyzing the presence and amount of RNA molecules in a given sample based on NGS technology. To estimate transcription quantity from RNA-Seq data, the sequence readings are aligned to the reference genome or transcriptome, and then the amount of expression is estimated by their alignment. Practically, various techniques are used for implementation. Estimation method for transcription quantity of each technique is slightly different. We focused on different features on estimation by different pipelines and compared between pipelines, and in particular, traced genes that differ significantly by pipelines. We distinguished several cases of features where the differentiation is made. We hope that this study will provide a more accurate estimate of quantification of transcripts in the field of crop research. *These authors contributed equally to this work. Corresponding Author: Tel , yjpark@kongju.ac.kr PA-158 Win Htet Oo 1, Yong-Jin Park 1,2 1 2 Rice is semi-aquatic, adapted to a wide range of hydrologies, from aerobic to anaerobic soils and flooded condition, to even deeply submerged soils in flood-prone areas. Flooding is a frequent hazard in lowland rice environments and is expected to intensify with climate change. Tolerance of anaerobic conditions exposed to complete submergence exploit two contrasting suites of traits, escape or quiescence, to survive this stress. In this study, comparison of anaerobic tolerance between two conditions, direct seeding and germinated seedling, to screen submergence tolerance variety from Korean Rice Heusristic Set. The quiescent and elongation strategies under control by the quantitative trait locus SUBMERGENCE 1 (SUB1) has been considered as a major mechanism controlling submergence tolerance in rice. Modern rice varieties are sensitive to flooding during germination and early growth but few landraces capable of germination under these conditions have recently been identified, enabling research into tolerance mechanisms. Further characterization of contrasting genotypes would help in elucidating the genetic and biochemical regulatory and signaling mechanisms associated with tolerance. Corresponding Author: Tel , yjpark@kongju.ac.kr 125

168 PA-159 Qiang He 1, Kyu-Won Kim 1, Weiguo Zhao 3, Yong-Jin Park 1, Improvement of rice eating and cooking quality (ECQ) is among the most important aims in current breeding programs. Genome-wide association studies (GWAS) and other methods have identified many loci associated with rice ECQ traits, however, many genetic variants influence complex traits by modulating gene expression, the biological mechanisms remain largely unknown. Here, we performed a transcriptome-wide association study (TWAS) to identify significant expression-trait associations with 10 ECQ traits using 84 Japonica rice accessions and 42,205 transcripts. There were 285 significant transcript associations for ECQ traits (FDR < 0.05), among 58 for ACC, 175 for PRO, 19 for PV, 16 for CS, 6 for PeT and 52 for PTemp. These associated genes mainly involved in the metabolic, biosynthesis of secondary metabolites and peroxisome pathways. For the genes associated with ECQ traits, variation in expression could be explained by expression quantitative trait loci (eqtls). Total 66,905 eqtls for 285 e-traits (p<1e-16), including 21,747 cis-eqtls (32.5%) and 45,158 trans-eqtls (67.5%), corresponding to 47,941 unique SNPs that affect the expression of 43 genes. The co-expression network for the genes associated with ECQ traits also constructed, 130 genes (45.6%) produced at least one network. Our results showcased the power of integrating genotype, gene expression and phenotype to gain insights into the genetic basis of ECQ traits, and will be providing an informative resource for studying rice ECQ traits molecular regulation mechanisms and be useful for rice molecular assistant selection breeding program aimed at developing new varieties with a high level of ECQ. Corresponding Author: Tel , yjpark@kongju.ac.kr PA-160 최홍집 1*, 오태영 2, 허창석 1, 이지윤 1, 배정숙 1, 김세종 우리나라찰옥수수생산규모는재배면적 15,000~20,000ha, 생산량은 80,000 톤정도이며전체시장규모는 3,000 억원정도로추정되고있다. 그러나재배되고있는대부분의옥수수가국내에서보급되고있는흰색찰옥수수이다. 본연구에서는베타카로틴, 지아산틴등기능성물질이함유된유색찰옥수수품종을개발보급하기위해 2002 년부터황색찰옥수수유전자원을수집하여교배및인공수분을통해계통을육성하여 2008 년도에 GYW1 과 GYW4 양친계통을육성하였고, 2011 년도에 GYW4 를종자친으로하고 GYW1 을화분친으로하여유망교잡종으로선발하였다 년부터 2013 년까지 2 년간특성평가와성능검정을수행하였으며 2014 년부터 2016 년까지 3 년간전국 5 개지역에서지역적응성시험을수행하여성능평가와균일성, 안정성시험을거처 황찰옥 으로품종명칭을정해 2016 년 12 월품종보호출원하였다. 황색찰옥수수신품종 황찰옥 은단교잡종으로웅수색과자수색이담녹색이며원통형이다. 이삭특성으로이삭길이는 18cm, 직경 4.2cm, 착립장률 98%, 평균이삭중 200g 정도였다. 10a 당이삭수는 6,543 개로표준품종인일미찰에비해 3% 많았다. 한편파종에서출사까지의소요일수는 62 일로표준품종인일미찰에비해 9 정도빨라조기수확이가능한품종으로확인되었다. 황찰옥 의채종시험결과모본과부본을 2:1 비율로파종하였을때 10a 당 132kg 을수확할수있었다. * 주저자 : Tel , chj1217@korea.kr 126

169 PA-161 김미진 1,2*, 문기범 1, 사미나단수브라야 2, 장지영 1, 배상수 3, 이긍주 2, 김현순 감자 (Solanum tuberosum L.) 는세계 4 대작물중의하나로서전세계인의중요한식량자원일뿐만아니라표준유전체가밝혀져연구모델로서도그가치가우수한작물이다. 감자에는솔라닌이라는천연독성물질이존재하는데, 이솔라닌은가지과에일반적으로있는 Glycoalkaloid 독성으로아린맛, 구토, 식중독, 현기증등사람에게부작용을유발할수있다. 수확후에도합성이진행되고취급방법이나저장조건에따라화합물이증가하는것으로알려져있다. 따라서맛과안전성을고려한감자의활용도증가를위하여연구가필요하고판단하였고, 유전자가위 CRISPR/Cas9(Clustered Regularly-Interspaced Short Palindromic Repeats/CRISPR associated protein 9) 을이용해솔라닌생합성에관여하는유전자를교정하여솔라닌생합성조절을위한기초연구를수행하였다. 유전자의교정여부를확인하는방법으로 Deep sequencing 과 HRM (High resolution melting) 을수행하였고, Deep sequencing 결과염기삽입, 결실등이일어났으며 0.2~2.2% 에변이율을확인하였다. HRM 분석결과에서는또한 Wild type 과 CRISPR/Cas9 처리한유전자간뚜렷한 Melting curve 차이를확인함으로서유전적변이가일어났다고판단할수있었다. * 주저자 : Tel , mijin364@kribb.re.kr PA-162 Paulina Calderón Flores, Dae Yeon Kim, Yong Weon Seo * Transcription factors (Tfs) naturally act as master regulators of cellular processes. They have broad functions such as regulation of floral organ development, photomorphogenesis, epidermal cell differentiation, hormonal responses, and flavonoid biosynthesis, also they act in concert with other regulations, the same transcription factor can be used repeatedly, with the outcome of the DNA binding event determined by which other regulators act in concert or opposition. The transcription complex composed of a WD40, a bhlh and a MYB protein regulates the expression of multiple distinct target genes in a range of plant species. It has been reported in rice, A. thaliana, petunia hybrida and Zea mays, that the bhlh interact with several MYB proteins. In this study, we performed transcriptome analysis of two samples of wheat seeds, Deep Purple (P) and Yellow (Y) during three developmental stages (early, middle, and late stage), 10 Days After Flowering (DAF), 20 DAF, and 30 DAF. Furthermore, we performed a Differential Expression Analysis to investigate which Tfs (MYB, bhlh or WD) are up or down-regulated and in which stage, and to analyze if there was difference between Yellow and Deep Purple samples. It seems that most of the Transcription Factors involved during the seed development between the Yellow and Purple samples are not the same and if the same Tf is shared, they are not expressing at the same time, we speculate that testa color is involved, further research need to be done to research if this Transcription Factors are working together to create some MYB-bHLH-WD complex to regulate gene expression. Acknowledgement: This study was supported by the Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ ), Rural Development Administration, Republic of Korea. Paulina Calderón Flores acknowledges CONACyT for financial support to follow doctoral studies. *Corresponding Author: Tel , seoag@korea.ac.kr 127

170 PA-163 Paulina Calderón Flores, Dae Yeon Kim, Yong Weon Seo * Transcription factors (Tfs) naturally act as master regulators of cellular processes. They have broad functions such as regulation of floral organ development, photomorphogenesis, epidermal cell differentiation, hormonal responses, and flavonoid biosynthesis. The transcription complex composed of a WD, a bhlh and a MYB protein regulates the expression of multiple distinct target genes in a range of plant species. It has been reported in rice, A. thaliana, petunia hybrida and Zea mays that the bhlh interact with several MYB proteins, furthermore in other plant species it has been reported the requirement for a WD-bHLH-MYB transcription complex for the control of anthocyanin biosynthesis. We performed transcriptome analysis during early [10 Days after Flowering (DAF)], middle (20 DAF) and late (30 DAF) stages of seed development to identify putative MYB-bHLH and WD transcription factors related to the flavonoid, more specific to the proanthocyanindin, pathway. We cloned and sequenced some putative genes and found some amino acid differences between samples, even insertions or deletions of several amino acids. We need to further analyze using yeast two hybrid to prove if actually there is interaction between these Transcription factors and know if they are working together in the flavonoid production, moreover, to investigate if the deleted or inserted amino acids are related to the interaction between these MYB-bHLH-WD complex function. Acknowledgement: This study was supported by the Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ ), Rural Development Administration, Republic of Korea. Paulina Calderón Flores acknowledges CONACyT for financial support to follow doctoral studies. *Corresponding Author: Tel , seoag@korea.ac.kr PA-164 Chan Seop Ko 1, Meong Kyu Ou 2, Jong Nae Hyun 2, Kyung Hun Kim 2, Jin Baek Kim 3, Min Jeong Hong 3, Yong Weon Seo 1* Hexaploid wheat (Triticum aestivum L.) represents about 30% of the world s cereal numbers, with over 220 million ha cultivated worldwide. Heat stress is one of the major environmental factors that give detrimental effect on crop yields as well as quality. The increased temperature during reproductive phase of plant growth has emerged as a serious problem all over the world. Korean wheat cultivars that were developed for various characteristics such as winter hardness, earliness, and pest resistance have been released since 1960s. However, heat stress which was minor consideration up to 1990s, is now increasing problem. We used Baegjoong which is developed for noodle making and its growing area is increasing. Plants were grown at optimum environment until 8 days after flowering. Plants that are subjected to heat stress are moved to phytotron set 33 /31 (day/night). The heat treated plants were brought back to normal environment until harvest. Whole spikes were collected on stressed periods (DAF8, DAF10, DAF12) and soothing period (DAF15) for total RNA extraction. The transcript results showed that most of the transcripts share treated and control, and only 45 and 6 high and low expression, respectively, during heat stress were found. Differentially expressed genes will be validated for further elucidation of gene functions. Acknowledgement: This work was carried out with the support of R&D agenda Agriculture & Technology development program (Project No. PJ ) Rural Development Administration, Republic of Korea. *Corresponding Author: Tel , seoag@korea.ac.kr 128

171 PA-165 Dae Yeon Kim 1, Min Jeoug Hong 2, Yong Weon Seo 1* 1 2 Hexaploid wheat is one of the most important cereal crops for human nutrition. Molecular understanding of the biology of the developing grain will assist the improvement of yield for different environments. In this study, we demonstrate transcript and protein expression analysis during seed development to understand the process of accumulation of phytochemicals in colored-wheat. The transcriptome from three different stages of developing spikelets in colored-wheat was determined using RNA sequencing. About 1200 and 3100 of significant transcripts (p-value < 0.05) from middle and late stages of grain development comparing with early stage of grain development were aligned to IWGSC1+popseq DB which is composed of over 110,000 transcripts including 100,934 coding genes. Gene ontology analysis were conducted and KEGG mapping was performed to show expression pattern of biosynthesis genes related in flavonoid, isoflavonoid, flavons and anthocyanin biopathway. Expression patterns of flavonoid biosynthesis pathway genes and candidate MYB bhlh WD40 (MBW) complex genes in RNAseq analysis during seed development were displayed by heatmap. Moreover, proteins shown high expression pattern at DAF 20 and DAF 30 compared to DAF 10 were selected by a k-means clustering method and identified by MLDI-TOF analsysis for peptide mass fingerprinting. Acknowledgement: This study was supported by the Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ ), Rural Development Administration, Republic of Korea. *Corresponding Author: Tel , seoag@korea.ac.kr PA-166 Eun Jeong Lee, Jin Seok Yoon, Yong Weon Seo * Wheat (Triticum aestivum L.), managing about 35% of the human population, is one of the most significant staple crops; moreover, consumption of crops has been increasing rapidly as the population grows over the decades, but the production rate can t keep up with the growing population. Therefore, advances in grain yields are the main ways to solve the challenge and meet the global demand. One approach for improving grain number and yield was the reduced plant height (dwarfing plants) that led to impressive increases in grain numbers and yields during the green revolution in wheat. It has been known that the genes associated with the dwarf stature in wheat are Reduced Height Genes (Rhts). Those dwarfing genes confer the reduction in stem elongation by a limited response to the phytohormone resulting in lodging resistance, improved harvest index, increase in partitioning of assimilates to the grain, more fertile spike, efficient utilization of the favorable environment, and yield benefits. In this study, genetic constitute of Rhts and their relationship with phenotype was investigated using F 2 segregation populations derived from crosses between Keumkang (tall) and 15DSPL014 (short line). Molecular markers that are relevant to dwarf genes are applied to individual plant for marker validation. The results could provide novel information about the effect of the dwarfing genes on plant height, and demonstrate potential availability for improving grain numbers and yields in wheat. *Corresponding Author: Tel , seoag@korea.ac.kr 129

172 PA-167 Cheol Won Lee, Yong Weon Seo * Flowering is a critical change which transit from vegetative stage to reproductive stage. In wheat, various environmental and endogenous cues determine flowering time. These factors and flowering pathways interact with each other and work as regulatory networks. Of these, vernalization, the induction of a plant's flowering process by exposure to the prolonged cold, specially works on flowering through the regulation of various vernalization genes and transcription factors. To clarify an influences of vernalization on the wheat flowering, we set experimental groups with different exposure length under low temperature. With this, we also analyzed other phenotype results such as leaf and shoot length. We used two Korean cultivars, Keumgang and Yeongkwang, which have different maturity. They were exposed under the 4, treated different period per five days. The longer wheat were exposed under low temperature, the more gap of flowering time was increased between two cultivars. However, spike primordium were ranked same stages at same wheat development stages although the treatment of vernalization period was different. The obtained results will provide valuable information to understand the role of vernalization for flowing regulation in wheat aimed at establishing the mechanism of wheat flowering. Acknowledgement: This work was carried out with the support of Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ ) Rural Development Administration. Republic of Korea. *Corresponding Author: Tel , seoag@korea.ac.kr PA-168 Woo Joo Jung, Yong Weon Seo * Cold stress is one of the most fatal abiotic stresses affecting the wheat production. Plants adjust to temperature changes by up-regulating cold-tolerant genes at non-freezing low temperature, which is called acclimation. Though wheat-rye translocation lines are known for their high adaptiveness to harsh environments and tolerance to biotic stresses, molecular mechanism in the process of acclimation is not fully understood. In the present study, we performed an RNA-seq analysis of 1BL.1RS translocation and its near-isonic line under normal and low temperatures (4 ). Total RNA extracted from the wheat seedlings were used to synthesize cdnas and construct a sequencing library. The high-throughput sequencing was done using the Illumina HiSeq 2500 platform. After trimming low-quality reads from raw reads, HISAT2 was utilized to map the raw reads to the IWGSC RefSeq v1.0. Gene function was annotated using BLAST2GO program based on NCBI nr protein database, InterProScan, Gene Ontology (GO) and KEGG pathways. Differentially expressed genes (DEG) with an adjusted p-value 0.05 and fold changes 2 were selected using featurecounts and DEseq. A Venn diagram analysis revealed that a certain group of genes was up-regulated specifically in 1BL.1RS under the cold treatment. Finally, real-time PCR analysis was performed to verify the DEGs. The results of this study show the process of gene expressions under low temperature in both 1BL.1RS and normal wheat. Further studies will be needed to fully investigate the roles of the identified genes in cold tolerance. Acknowledgement: This work was carried out with the support of Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ ) Rural Development Administration. Republic of Korea. *Corresponding Author: Tel , seoag@korea.ac.kr 130

173 PA-169 Tae-Heon Kim 1, Su-Min Jo 1, Saes-Beul Lee 1, Ji-Yoon Lee 1, Sang-Ik Han 1, Jun-Hyeon Cho 1, Jong-Hee Lee 2, You-Chun Song 1, Dong-Soo Park 1, Myung-Kyu Oh 1, Dongjin Shin *1 1 2 Rice is a staple food crop for more than half of the world population. Rice production is largely affected by various environmental conditions such as cold, heat and flooding annually. Especially rice is a highly sensitive to low temperature below because of originating from tropical or subtropical climates. Seedling of rice is easily damaged to low temperature and result in yellowing, growth retardation, reduced tillering, which can cause severe yield losses. In addition, more frequent and intense cold damage of rice has been occurred in recent years by global climate change. A sharp decrease in rice production on account of cold damage constitute a threat to global food security. We used a recombinant inbreeding lines (RIL) population of 384 individuals derived from a cross between Hanareum 2, a highly cold sensitive variety and Unkwang, a cold tolerant variety for molecular mapping of QTLs related to cold tolerance. Seedling discoloration of each lines and parents caused by cold response were investigated in field condition after transplanting in 2016~2017. The seedling of each lines and parents was subjected to low temperate by 5~13 during 14 days. The cold recovery score (CRS) of RILs was recorded after 4 days recovery period according to standard evaluation system (SES, IRRI). Total of 10 QTLs were detected on chromosome 1, 6, 7, 8, 10, 11 and 12 using cold tolerance traits, seedling discoloration and cold recovery score in 384 RILs. The qcrs12, which detected on chromosome 12 between two flanking markers id , id (1.1 Mbp) showed 25 LOD score with 26% of phenotypic variation of cold recovery score in RILs population. The positive allele contributing to cold tolerance came from the cold tolerant parent Unkwang. The result may provide useful information for a marker-assisted breeding program to improve cold tolerance in rice. *Corresponding Author: Tel , jacob1223@korea.kr PA-170 Jong-Yeol Lee *1, You-Ran Jang 1, Susan B Altenbach 2, Sun-Hyung Lim 1, Young-Mi Kim The accurate identification of alleles for high-molecular weight glutenins (HMW-GS) is critical for wheat breeding programs targeting end-use quality. RP-HPLC methods were optimized for separation of HMW-GS, resulting in enhanced resolution of 1By and 1Dx subunits. Statistically significant differences in retention times (RTs) for subunits corresponding to HMW-GS alleles were determined using 16 standard wheat cultivars with known HMW-GS compositions. Subunits that were not identified unambiguously by RP-HPLC were distinguished by SDS-PAGE or inferred from association with linked subunits. The method was used to verify the allelic compositions of 32 Korean wheat cultivars previously determined using SDS-PAGE and to assess the compositions of six new Korean cultivars. Three cultivars contained subunits that were identified incorrectly in the earlier analysis. The improved RP-HPLC method combined with conventional SDS-PAGE provides for accurate, efficient and reliable identification of HMW-GS and will contribute to efforts to improve wheat end-use quality. *Corresponding Author: jy0820@korea.kr 131

174 PA-171 Jong-Yeol Lee *1, You-Ran Jang 1, Sun-Hyung Lim 1, Kyoungwon Cho 1 1 Wheat gliadins confer the dough elasticity and extensibility through forming the gluten complex with glutenins and contain dominant epitopes to cause celiac disease (CD) and wheat-dependent exercise-induced anaphylaxis (WDEIA). The gliadins, including α-/β-, γ- and ω-gliadins, in hexaploid wheat cultivars are encoded at Gli-1, Gli-2 and Gli-3 loci on homoeologous group 1 and 6 chromosomes. The large sizes of their gene families, the similar genetic structure and the large proportion of pseudogenes and the lack of genomic data make it difficult to study the composition of gliadin in hexaploid wheat variants. In this study, to challenge the difficulty using the improved proteomic tools, we extracted ethanol-soluble gliadin fractions from flour of a Korean wheat bread cultivar Keumkang (Triticum aestivum cv. Keumkang). Total 98 spots from the fraction were separated on 2-DGE gel, digested with chymotrypsin and then subjected to a nanouplc qtof-ms/ms. Using Scaffold program linked to NCBI Triticeae and Butte 86-specific protein databases, α -/β -gliadins, γ -gliadins, LMW-GSs, ω -gliadin and non-gluten proteins were identified to be present as the major protein in thirty-one (40.8%), twenty-eight (27.7%), eleven (10.6%), one (0.5%) and seven spots (5.3%) of 96 spots, respectively. The composition and repetitive number of CD- and WDEIA-relevant epitopes in the gliadin sequences indicate the potential riskicity of wheat cv. Keumkang flour in patients with CD and WDEIA and suggest the way forward for improving the food processing qualities and reducing the gliadin-derived adverse health effects. *Corresponding Author: jy0820@korea.kr PA-172 Jong-Yeol Lee *1, You-Ran Jang 1, Sun-Hyung Lim 1, Kyoungwon Cho 1 1 Seed storage proteins (SSPs) such as glutelin, prolamin and globulin are abundant components in some of the most widely consumed food cereals in the world. Synthesized in the rough endoplasmic reticulum (ER), SSPs are translocated to the protein bodies. Prolamins are located at the spherical protein body I derived from the ER, whereas glutelins and globulin are accumulated in the irregularly shaped protein bodies derived from vacuoles. Our previous studies have shown that the individual suppression of glutelins, 13-kDa prolamins and globulin caused the compensative accumulation of other SSPs. Herein, to investigate the phenotypic and molecular features of SSP deficiency transgenic rice plants suppressing all glutelins, prolamins and globulin were generated using RNA interference (RNAi). The results revealed that glutelin A, cysteine-rich 13-kDa prolamin and globulin proteins were less accumulated but that glutelin B and ER chaperones, such as binding protein 1 (BiP1) and protein disulfide isomerase-like 1-1 (PDIL1-1), were highly accumulated at the transcript and protein levels in seeds of the transformants compared to those in the wild-type seeds. Further, the transcription of starch synthesis-related genes was reduced in immature seeds at two weeks after flowering, and the starch granules were loosely packaged with various sphere sizes in seed endosperms of the transformants, resulting in a floury phenotype. Interestingly, the rates of sprouting and reducing sugar accumulation during germination were found to be delayed in the transformants compared to the wild-type. *Corresponding Author: jy0820@korea.kr 132

175 PA-173 Da-Hye Kim *1, Jung Ae Park 1, Sung Ok Park 1, Sang-Kyu Park 1, Jong-Yeol Lee 1, Sun-Hyung Lim 1 1 Flower color is one of the main target traits in the flower breeding. To production of novel flower color in transgenic plants, it requires transgene that confer a useful trait and flower-specific promoters. To get the flower specific promoter, we focused on the late biosynthetic gene of anthocyanin, which is exclusively accumulated in flower of tobacco plant. Anthocyanidin synthase (ANS), a key enzyme in the late stages of the biosynthesis of anthocyanins, was cloned from flowers of the tobacco plant (Nicotiana tabacum). Analysis of the spatial regulation of expression of tobacco ANS gene has revealed that levels of its transcript are particularly abundant in flower tissues. To investigate activity of the promoter of the NtANS1 gene, transgenic tobacco plants with β -glucuronidase (GUS) under the control of the NtANS1 promoter (NtANS1-P) exhibited the authentic flower-specific expression. To enhance flower colors, the mpap1 and B-Peru TF genes were both expressed in tobacco plant under NtANS1-P. Transgenic tobacco plants showed the normal growth and distinct reddish colors in flowers compared to nontransgenic tobacco plants. Structural genes and regulatory genes of anthocyanin biosynthetic pathway were significantly upregulated in transgenic tobacco flower. Consistent with gene expression profiles, anthocyanin content was significantly higher in transgenic tobacco flower. This result suggests that combination both of target trait gene, TFs and flower-specific promoter, NtANS1-P can show a reddish flower color in transgenic tobacco without any detrimental phenotype such as growth retardation. In conclusion, it might have valuable components for flower color modification through metabolic engineering. *Corresponding Author: kimdh143@naver.com PA-174 You-Ran Jang *1, Sun-Hyung Lim 1, Jong-Yeol Lee 1 1 Gliadins, which account for 40-50% of total proteins, contribute to viscosity and elongation and play an important role in the formation of wheat dough. The genes encoding gliadins are mainly located on the short arms of chromosome 1 and 6. Gliadins are divided into four groups ω 5-, ω 1,2-, α -/β -, γ -gliadin. The ω 5-gliadin, encoded on wheat chromosomes 1, is the major antigen of wheat dependent exercise-induced food anaphylaxis (WDEIA). γ -gliadin encoded on chromosome 1 and α - / β -gliadin present on chromosome 6 are the major antigens of celiac disease. We conducted RP-HPLC, SDS-PAGE, A-PAGE and 2-DGE in gliadin fractions using Chinese Spring and its aneuploid lines of chromosome 1 and 6 to assign the individual gliadins to their chromosome encoded. In RP-HPLC, all peaks except one peak could be explained, but much could not be explained in SDS-PAGE, A-PAGE and 2-DGE. In particular, 11 out of 33 gliadin spots were not assigned in 2-DGE experiments. From the above results, we conclude cautiously that there might some gliadin proteins encoded on other chromosomes than chromosome 1 and 6. To prove this, additional experiments are required with the MS / MS identification of these 11 spots and analysis of CS aneuploid lines except for chromosome 1 and 6 as further study. *Corresponding Author: jang6122@jbnu.ac.kr 133

176 PA-175 Su-Jin Cho *1, Jin Sun Kim 1, You-Ran Jang 1, Sun-Hyung Lim 1, Jong-Yeol Lee 1 1 Glutenin is a protein that is soluble in alkali even in gluten, which is a major component of wheat storage proteins. Glutenin is divided into high-molecular weight glutenin subunits (HMW-GS) and low-molecular weight glutenin subunits (LMW-GS) according to molecular weight size in SDS-PAGE. The effect of each alleles of glutenin on wheat quality properties is very important, each alleles is different from each other. Therefore, we need to know and identify each exact allele. In particular, LMW-GS plays an important role in the extensibility of wheat. On the other hand, HMW-GS alleles have been studied to some extent. However, LMW-GS allele studies are very insufficient and difficult to identify exact alleles of LMW-GS because there are many variations of allele and mobility is overlapped with gliadin fraction. In previous studies, the standard cultivar was checked by 2-DGE for the LMW-GS allele assay, but it was not clearly distinguishable. In this study, a comprehensive allele was analyzed by both 2-DGE and RP-HPLC experiments using the Aroona LMW-GS NILs (near isogenic lines) for more accurate and rapid LMW-GS allele validation. NIL refers to one line in which only one target LMW-GS allele is different and the remaining genetic composition is same. As a result of comparison of 2-DGE and RP-HPLC in the Aroona NILs, it was possible to establish more accurate alleles than existing alleles which was not distinguished by standard cultivars. These results are expected to contribute faster screening of improved wheat varieties development when breeders identify alleles. *Presenting Author: Tel , bartbox@naver.com PA-176 Mummadi Reddy Ramya, Hye Ryun An, Pue Hee Park * Flower volatiles are valuable for many floricultural crops. Floral scents play key role to attract the pollinators and ensure the fertilization. The genus Cymbidium belongs to the Orchidaceae family and is economically important due to many species having beautiful and fragrant flowers. Cymbidium cultivar Sunny Bell flowers are highly fragrant and small size pink flowers. Based on the richer volatiles in this plant we needed to isolate the floral scent pathway full length genes. In this paper, three genes encoding 1- deoxy-d-xylulose-5-phosphate synthase (DXS), 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR), and linalool synthase (LIS) involved in the monoterpene biosynthesis pathways were cloned and sequenced by using topo cloning kit. In functional characterization LIS gene 81% matches Rose, and DXS and DXR sequences similar to Ricinus communis and Hevea brasiliensis. The variations in the constituent and content of volatile monoterpenes including linalool and geraniol For expression analysis among the three genes LIS were highly expressed in all flower stages. In addition DXS, and DXR also expressed in full flowering stages at low level and down regulated in early developmental stages. According to bioinformatics analysis the deduced amino acid sequences were 382, 342 and 359 respectively. However the full length floral scents genes which as LIS, DXR and DXS highly expressed in all floral organs and floral developmental stages. According to these results we confirmed that monoterpenoid pathway might be leading pathway. In further we will focus on regulation model through floral scent in Cymbidium cultivar Sunny Bell. 134

177 PA-177 Seon-Ju Shin 1, Jae-Hee Lee 1, Hongryul Ahn 2, Inuk Jung 3, Sun Kim 2,3, Hawk-Bin Kwon 1* Nitrogen concentration in soil is one of the main factors directly affecting crop yield. However, the use of large amounts of nitrogen fertilizer to promote growth causes environmental problems. Therefore, we sought to identify genes involved in nitrogen metabolism that could improve the nitrogen metabolism efficiency in rice. In this study, we identified several novel nitrogen-use-efficiency (NUE) regulatory coding and noncoding transcripts in Oryza sativa L., using the next generation sequencing (NGS) technique and bioinformatics analyses. We investigated the functions of the activated/suppressed differentially expressed genes under nitrate treatment by Gene Ontology (GO) enrichment analysis. We identified the coding and noncoding transcripts (and clusters) that responded differently upon increasing or decreasing the nitrogen concentration. We then analyzed the transcription factors controlling the expression of these gene clusters through gene module analysis, and analyzed interactions between coding and noncoding genes through gene expression networking *Corresponding Author: Tel , hbkwon@sunmoon.ac.kr PA-178 Chaeyoung Lee 1, Jin-Hyun Kim 1, Joo-Seok Park 2, Yoram Choi 2, Min-Gyun Jeong 2 and Hong-Kyu Choi 3* The Fabaceae (legume family) is the third largest and the second of agricultural importance among flowering plant groups. In this study, we report the reconstruction of a composite comparative map composed of ten legume genomes, including seven species from the galegoid clade (Medicago truncatula, Medicago sativa, Lens culinaris, Pisum sativum, Lotus japonicus, Cicer arietinum, Vicia faba) and three species from the phaseoloid clade (Vigna radiata, Phaseolus vulgaris, Glycine max). To accomplish this comparison, a total of 209 cross-species gene-derived markers were employed. The comparative analysis resulted in a single extensive genetic/genomic network composed of 93 chromosomes or linkage groups, from which 110 synteny blocks and other evolutionary events (e.g., 13 inversions) were identified. This comparative map also allowed us to deduce several large scale evolutionary events, such as chromosome fusion/fission, with which might explain differences in chromosome numbers among compared species or between the two clades. As a result, useful properties of cross-species genic markers were re-verified as an efficient tool for cross-species translation of genomic information, and similar approaches, combined with a high throughput bioinformatic marker design program, should be effective for applying the knowledge of trait-associated genes to other important crop species for breeding purposes. Here, we provide a basic comparative framework for the ten legume species, and expect to be usefully applied towards the crop improvement in legume breeding. *Corresponding Author: Tel , hkchoi@dau.ac.kr 135

178 PA-179 Nomar Espinosa Waminal 1,2, Remnyl Joyce Pellerin 1, Sung-Min Youn 1, Tae-Jin Yang 2* and Hyun Hee Kim 1* 1 2 Fluorescence in situ hybridization (FISH) is a highly efficient molecular cytogenetic tool to visualize distribution of DNA elements in a genome. Conventional FISH procedure involves preparation of fluorescence-labeled probes that is time-consuming and requires technical experience. To improve this process, we designed oligodeoxynucleotide (ODN) probes for three commonly used targets, 5S, 45S rdna, and telomere repeats. We designed twelve, four, and one ODN probes for 45S rdna and 5S rdna, and telomere tandem arrays, respectively. The rdna probes has universal utility for plants including gymnosperms and angiosperms, animals, and fungi and telomere probe can be utilized for most of plants harboring Arabidopsis-type, (TTTAGGG)n, telomere array. Use of different fluorochromes conjugated to the 5 end of each ODN probes permitted the simultaneous visualization of the genomic loci of the three target sequences which are commonly the most distinct feature of a chromosome complement. Utilization of pre-labelled ODN probes reduced efforts and time for FISH analysis. It greatly reduced the FISH hybridization time from ~16h at 37 for conventional FISH to as short as 5 min at room temperature. The ODN probes can be ideal for routine FISH analysis even for species without preliminary chromosomal data. The method is efficient, reliable, and rapid in simultaneously detecting several target repeats, and is particularly useful in rapid chromosomal analysis of crop collections in breeding studies. Acknowledgement: This work was carried out with the support of "Cooperative Research Program for Agriculture Science & Technology Development (PJ )" Rual Development Administration, Republic of Korea. *Corresponding Authors: Tae-Jin Yang: Tel , , tjyang@snu.ac.kr Hyun Hee Kim: Tel , kimhh@syu.ac.kr PB-01 Inyoung Kim, Hyun Uk Kim * Fibrillin (FBN) 1a, 1b and 2 are lipid-associated proteins of plastids and accumulated under abiotic stress. In high light stress, FBN1a and 1b accumulation are regulated by abscisic acid (ABA) response regulators ABA-insensitive 1 (ABI1) and ABI2. It enhances resistance of photosystem II photoinhibition. The phenotype of triple FBN1a,1b,-2 suppressed transgenic plants generated by RNA interference (RNAi) using common sequences from three FBN1a, 1b and 2 show a lower shoot growth development and reduced anthocyanin accumulation under high light/cold stresses. These phenotype of transgenic plants are restored by jasmonate (JA) treatment. As a results, FBN1a,1b, and -2 are expected to mediate by JA biosynthesis. In previous research, the functions of FBN2 is not well known. Our data suggest that the FBN2 interacted with PTM, a chloroplast envelope-bound transcription factor involved in retrograde signaling to mediate activation ABI4 transcription. To investigate detail molecular function of FBN1a,1b and -2 under the high light/cold stress, we generated each of single and double mutants of fbn1a and 1b using selection of T-DNA insertional mutants, and FBN2 suppressed transgenic plant of using FBN2-RNAi. Also, we are generating triple mutants of fbn1a,1b,-2 combined with CRISPR/Cas9 gene editing system. *Corresponding Author: Tel , hukim64@sejong.ac.kr 136

179 PB-02 Kwang-Soo Cho *, Min-Kwon, Hong-Sik Won, Ji-Hong Cho, Ju-Seong Im, Jang-Gyu Choi and Young-Eun Park Trichomes are specialized epidermal structures that protect plants from abiotic and biotic stresses. Cultivated potato (Solanum tuberosum) is known to have glandular and non-glandular trichomes. However, the classification of trichome types have not been studied in detail. In addition, little is known about what types of tirchoms exist in its wild species. Here, one representative cultivated potato and 17 wild potato species were examined with FESEM (Field Emission Scanning Electron Microscopy) to compare trichome types, morphology, and density. Cultivated potato has two glandular and two non-glandular trichome types based on their morphology. All wild potato species tested here also have similar trichome types and have no unique trichome types. Among solanaceous species tomato trichome types (I~VII) were well characterized. So we designated potato trichome types with tomato trichome types. Previously known potato glandular type A and B (tomato trichome type VI and VII) were characterized by a very short stalk with irregularly shaped multi-celled heads (4 to 8) and by a short multi-cellular stalk with four-celled globular heads, respectively. Two potato non-glandular trichomes, previously not named, were characterized by a long multi-cellular stalk with multi-cellular bases (tomato trichome type II) and by a multi-cellular stalk with unicellular base (tomato trichome type III), respectively. Based on the trichome density, we could classified wild relative potato clones into four groups. First group (2 species) S. berthaultii and S. hugaii have high density of glandular type in abaxial and adaxial side. Second (1 species), S. mochiquense have high density only non-glandular type trichomes only in abaxial side Third group (13 species), S. raphanifolium, S. jamesii, S. cardiophyllum, S. hjertingii, S. stoloniferum, S. acaule S. brevicaule, S. chacoense, S. kurtzianum, S. verrucosum, S. pinnatisectum, S. iopetalum have similar density of glandular and non-glandular type trichomes that of S. tuberosum. Fourth group (2 species), S. microdontum and S. verni have high density non-glandular type trichomes in abaxial and adaxial sides. To test infestation degree by aphids (Macrosiphum euphorbiae), four species include S. tuberosum were used. The lowest number (13.1) and highest (30.0) of honey dew spots secreted by aphids was shown in S. hugasii and S. mochiquense, respectively. The degree of infestation by aphids was strongly negative correlated with total number of glandular trichome in potato abaxial and adaxial leaf sides. Acknowledgement: This work was supported by a grant from the Next-Generation BioGreen 21 Program (Plant Molecular Breeding Center No. PJ011841), Rural Development Administration, Republic of Korea *Corresponding Author: Tel , kscholove@korea.kr PB-03 조성우 1, 김학신 2, 최용현 3, 최용석 3, 박철수 1* 밀의전분특성은국수와빵의부드러운식미를결정하는주요인자로주로아밀로스함량에영향을받는다. 그러나, 비슷한아밀로스함량이라고하더라도전분의팽윤 (Swelling) 과호화 (Pasting) 및노화 (Staling) 특성은다양하게나타나기때문에아밀로스함량측정만으로식미가우수한자원선발에는한계가있다. 그러므로본연구에서는전분특성을평가하는방법간비교를통하여육종에효율적으로이용이가능한분석방법을제시하고자한다. 본연구에는 2014 년과 2015 년에수확한 15 개품종에대해서밀가루와전분의팽윤및호화특성을비교분석하였다. 밀가루와전분의팽윤및호화특성은년차간변이보다는품종간변이에영향을많이받는것으로나타나서이들특성을육종프로그램의선발지표로이용이가능할것으로보인다. 아밀로스함량은팽윤특성과높은상관을나타내었으며, 호화특성에서도 setback 값을제외하고높은상관을나타내었다. 밀가루와전분의팽윤특성간에는높은정의상관을나타내었고, 호화특성에서는식미특성과높은상관이있는 peak viscosity 와 breakdown 이밀가루와전분간에높은정의상관을나타내었다. 이를통하여단백질및전분함량이밀가루의팽윤및호화특성에영향을줄수는있지만전분과밀가루의전분특성결과간에높은상관이나타나기때문에육종프로그램에서는밀가루를이용한전분특성이크게문제가되지않을것으로생각되며, 분석에시료가적게드는팽윤특성이육종프로그램에이용이보다효율적일것으로보인다. 그러나팽윤부피와무게 (Swelling volume and power) 분석간차이는발견하지못했다. 그러나밀가루를이용한팽윤특성이육종프로그램에이용된다고하더라도제분과정이필요하기때문에실제육종에서이용하기위해서는통밀 (Wholemeal) 분쇄를이용한팽윤특성분석을실시가필요하다. 통밀을이용할경우여러가지요인을고려해야하기때문에이에대한연구는추후진행하고자한다. * 주저자 : Tel , pc89@jbnu.ac.kr 137

180 PB-04 조성우 1, 김학신 2, 최용현 3, 최용석 3, 박철수 1* 믹소랩은반죽의단백질특성뿐만아니라전분화호화및노화특성을평가할수있기때문에반죽물성을평가하는믹소그래프나파리노그래프와전분호화특성을평가하는아밀로그래프를이용한평가를동시에할수있는장점이있다. 믹소랩분석결과, 국내밀품종의가수량과반죽시간은연질밀이나박력분과비슷하였으나, 반죽의안정도그리고단백질연화정도는경질밀이나중강력분수준을보였다. 믹소랩으로측정된국내밀품종의전분특성은경질밀의전분특성과유사함을보였다. 믹소그래프와믹소랩의상관에서반죽의가수량은정적상관을보인반면에반죽시간이나안정도는상관이없는것으로확인되었다. 아밀로그래프의최고점도와브레이크다운은호화및조리의안성도그리고노화정도와상관을보였다. 믹소랩의반죽및단백질특성은빵부피, 삶은국수의경도및과자직경과높은상관을보였고, 삶은국수의탄성및점성은단백질특성및전분의호화정도와노화특성과상관이있는것으로나타났다. * 주저자 : Tel , pc89@jbnu.ac.kr PB-05 이원도 1, 강시용 2, 박용진 3, 권순욱 4, 이영상 5, 조유현 1* 스위트드림 1 세 는밥쌀용쌀소비감소와쌀재고량증가로인한쌀산업위기를극복하고자소비자요구에다양성을제공하고, 당질미를활용한다양한가공적성쌀품종을공급하기위하여농업회사법인주식회사시드피아에서육성되었다 년해외수집조생찰의 sugary 변이체 (KNUR42225M(1132) ) 를부본으로하고화영벼모본으로하여교배하여 2008 년부터 2014 년까지매년포장에서계통재배하면서찰벼이며당질미특성을갖는계통을선발, 유전적으로온전히고정하였다. 선발된계통을 SP47 로계통명을부여하고, 2015 년 ~ 2016 년에난괴법 3 반복으로생산력검정을수행하여품종의균일성과안정성을확인하여 스위트드림 1 세 라고명명하였다. 스위트드림 1 세 는중부지역보통기재배에서 8 월 20 일경에출수하는중만생종으로간장이 79.2cm 이며주당수수 14.5 개정도이다. 현미천립중은 19.0g, 장폭비 1.70 로단원형이다. 백미수량은 418 kg /10a 로추청벼대비 80% 수준으로기존당질미에비해대단히높은편이다. 특히 스위트드림 1 세 는유리당함량이 5.5% 로일반품종에비해 5 배가량높으며, 식이섬유함량도 100g 당 10.91g 로다량으로함유하고있어가공식품원재료로활용이기대된다. * 주저자 : Tel , seedpia.001@gmail.com 138

181 PB-06 이원도 1, 강시용 2, 박용진 3, 권순욱 4, 조유현 1* 예농 2 호 는고기능성거대배신품종육성을목적으로육성되었으며, 2007 년하계에신동진거대배돌연변이체를모본으로하고, 변이모본인다수확, 내도복특성을갖는신동진벼를부본으로여교배하여 2007 년부터 2009 년까지계통재배하면서거대배특성을갖고, 임성, 초형이양호한 KJ 계통을선발고정하였다. 선발된계통을 YN-007 로계통명을부여하고, 2011 년 ~ 2012 년에난괴법 3 반복으로생산력검정을수행하여품종의균일성과안정성을확인하여 예농 2 호 라고명명하였다. 예농 2 호 는중부지역보통기재배에서 8 월 18 일경에출수하는중만생종으로간장이 87.1cm 이며주당수수 12.8 개정도이다. 현미의장폭비는 1.81 의중원형이고, 현미천립중은원품종인신동진벼 27.7g 보다가벼운 23.6g 수준이다. 그러나예농 2 호의쌀눈무게는신동진벼보다 2.8 배무거웠다. 예농 2 호는 α -tocopherol 이대조품종보다 2.5 배높은함량을보였고, 전토코페롤함량에서도 2 배정도로차이가났으며, δ -tocopherol 은 6.5 배수준으로나타났다. * 주저자 : Tel , seedpia.001@gmail.com PB-07 이원도 1, 김태형 2, 박용진 3, 권순욱 4, 이영상 5, 조유현 1* 천지향 1 세 는글로벌소비자기호에적합하고농가소득향상과함께미래식문화변화에대응하고자우수한향특성이가미된품종개발을목적으로농업회사법인주식회사시드피아에서육성되었으며, 2004 년하계에초형이우수하고약한정도의향을갖는 HJ-11 계통을모본으로하고, 현미외관품위가양호하고약한정도의향을갖으며, 초형이우수한 J-42 를부본으로교배하여 2004 년부터 2012 년까지매년포장에서계통재배하면서유전적으로온전히고정된계통을선발, 고정하였다. 선발된계통에대해 2013 년 ~ 2015 년에난괴법 3 반복으로생산력검정을수행하여품종의균일성과안정성을확인하여 천지향 1 세 라고명명하였다. 천지향 1 세 는중부지역보통기재배에서 8 월 19 일경에출수하는중만생종으로간장이 79.3cm 이며주당수수는 15.4 개정도이다. 현미천립중은 18.6g, 장폭비 1.71 로단원형이다. 메벼로서현미의아밀로스함량과단백질함량은각각 19.3%, 6.5% 정도이며, 백미수량은 5.28MT/ha 로추청벼와비슷한수준이다. 천지향 1 세 는쌀에서은은한팝콘향이나는향미품종으로재배확대가기대된다. * 주저자 : Tel , seedpia.001@gmail.com 139

182 PB-08 홍지화 1*, 이영길 1, 강지선 2, 이민휘 1, 박영준 1, 김현태 1, 이재훤 1, 김동민 양곡관리법의양곡의혼합금지조항에따라생산연도 ( 연산 ) 가다른양곡 ( 쌀, 현미 ) 의혼합이금지되어있으나신곡과구곡을혼합하여거짓으로표시하는사례가증가하고있어이를판별할수있는방법개발이필요한실정이다. 현미는쌀에비해영양성분이풍부하고발아가되기때문에이를이용하여현미의생산연도판별이가능한지에대한연구를수행하였다. 일품 품종의연산별시료 (2012 년산, 2013 년산, 2014 년산, 2015 년산각 10 점 ) 를대상으로국제종자검정협회의발아율검정방법을이용하여발아율을조사한결과각연산별평균발아율은각각 0%, 7%, 56%, 97% 로나타나생산연도별시료의발아율에차이가나타났고통계분석결과신곡과구곡간에유의한차이를나타내었다. 발아율과생산연도판별의상관성을알아보기위하여상주의양곡보관창고를지정하여저장기간 (6 개월, 8 개월, 10 개월, 12 개월 ) 과저장조건 ( 상온, 저온 ) 에따른현미의발아율을시험하였다. 동일한시료라도상온보관에비해저온보관시발아율이더높게나타났고저장기간이길어질수록발아율이낮아지는경향이었다. 상온조건에보관된시료의저장기간에따른현미의발아율을검정한결과시료를수확시점으로부터 8 개월정도보관한시료는 2015 년산과 2014 년산간에유의차가없었으나, 10 개월에서 1 년간보관시에는신곡과구곡이통계적인유의차를나타내었다. 반면저온조건에보관된시료의경우는 6 개월정도보관시에는 2015 년산과 2014 년산간에유의차가없었으나 8 개월에서 1 년간보관시에는신곡과구곡간에유의차를나타내었다. 시료를단기간보관시에는상온과저온조건이신곡과구곡의발아율에크게영향을주지않았으나시료를장기간보관시에는저장온도조건에따라신곡과구곡시료의발아율에영향을주는것을알수있었다. 본연구에서수집한일품벼의연산별발아율은각연산간에발아율범위가중복되지않은것으로나타나향후현미의단일연산또는신 구곡을판별하기위한기초자료로활용이가능할것으로사료된다. * 주저자 : Tel , hongjh19@korea.kr PB-09 홍지화 1*, 이솔화 2, 손민식 2, 박영준 1, 김현태 1, 이재훤 쌀의신곡과구곡의판별을위한지표물질로기존에활용중인인지질지표물질외에신규지표물질을탐색하기위하여안정동위원소분석, 무기원소분석, 유기성분분석, 전자코분석을시도하였다. 하지만신곡과구곡단일곡에대한판별정확도가 95% 이하로나타나지표물질로선발하지못하였다. 이에국립농산물품질관리원에서고시한쌀의신선도감정법을신곡과구곡의판별법개발연구에적용해본결과신곡과구곡인단일곡의경우 95% 이상의정확도로판별되는것으로나타났다. 신선도감정법은 Guaiacol, Oxydol, p-phenylenediamin 3 종의시약처리를통하여쌀에포함된효소의산화효소작용의정도를알아보는방법으로발색된시약의착색정도를육안으로감정하는방법으로규정되어있다. 따라서효소활성도를쌀의신곡과구곡판별을위한지표물질로추가선정하였으나본감정법은시약을조제하는사람에따라감정결과가상이하고, 시약조제에시간이많이소요되는등현장업무에직접활용하기에는제약이존재하여현재의방법에대한신속화와검정표준화시스템구축이필요한실정이다. 이에현장휴대용으로활용하기위하여기존의시약원액을이용하여수집된시료에대한시약조제별안정성시험을수행하였고 Guaiacol 1%, p-phenylenediamin 0.2% 를기질로 Sodium Perborate 1% 에안정제를첨가한시약을개발하였다. 개발된키트를이용한검정결과는기존에시약을직접조제하여검정한결과와동일한착색반응을나타내었다. 본연구를통해개발된키트는쌀의신선도뿐만아니라신곡과구곡단일곡을판별하는도구로활용이될것으로기대된다. * 주저자 : Tel , hongjh19@korea.kr 140

183 PB-10 홍지화 *, 최경후, 김은옥, 연영자, 박영준, 김현태, 이재훤 풋고추농산물의매운맛성분에대한표준규격개발을위하여시중유통풋고추 111 점을수집한후매운맛함량을분석하여매운맛규격설정이가능한지알아보았다. 풋고추캡사이신추출방법을확립하기위하여풋고추꼭지와씨를제거한후건조시켜분쇄후고춧가루 KS 규격설정시활용된매운맛분석법을활용하여분석조건을확립하였다. LC 분석결과고추매운맛표준물질인 capsaicin 및 dihydrocapsaicin 의피크머무름시간 (Retention time, RT) 은각각 4.2 분, 6.8 분으로나타났고표준물질검량식의상관계수값 (R 2 ) 값은 이상으로나타났다. 풋고추공시시료의매운맛함량은 4.4ppm ppm 의범위를나타났고평균매운맛함량값은 967.4ppm 으로나타났다. 풋고추에표시된상품별평균매운맛함량값 (ppm) 은풋고추 (697.9), 모닝고추 (43.9), 롱그린 (48.2), 청양 (2494.8), 오이맛 아삭이 (95.2), 매운고추 (3521.4), 과일 (23.2), 애기초 (398.6) 로분석되었고분석횟수간의상대표준편차 (% RSD) 값은 로나타나재현성과정밀도가높게나타났다. 풋고추포장재표시사항별평균매운맛함량을살펴보면풋고추 ( 품종포함 ) 로표기된시료의매운맛함량분포는청양고추, 매운고추, 오이맛 아삭이, 과일고추, 애기초고추, 모닝고추, 롱그린고추의매운맛분포범위를모두포함하여매운맛정도의변이가큰것으로파악되었다. 따라서시중에유통되는풋고추의매운맛수치는값의변이가존재하여향후등급안 ( 매우매운맛, 보통매운맛, 순한맛등 ) 마련이가능할것으로판단된다. 또한공시시료를대상으로 CS(capsaicinoid synthetase) 유전자포함유무를분석한결과 111 점모두연관마커에서증폭이되는것으로나타났다. 본연구를기반으로풋고추의매운맛성분에대한표준규격이설정된다면풋고추농산물의품질관리뿐만아니라매운맛성분육종등의분야에도유용하게활용될것으로사료된다. * 주저자 : Tel , hongjh19@korea.kr PB-11 이민석 1*, 고건희 1, 이정대 1, 유주 1, 이진호 1 1 제주도소재오설록연구소에서고기능성고품질차나무신품종개발을위하여한국, 일본, 중국에서수집된차나무 (Camellia sinensis L.) 유전자원에대하여작물학적특성과잎의기능성성분및재차품질특성에대한평가를실시하였다. 그결과 2007 년 4 월에기능성성분과품질이뛰어난유전자원에대하여 1 차선발을실시하였고, 삽목방법을통하여계통육성을실시하였다. 이렇게정식을한 21 개의계통을성목이될때까지 3 년간 (2008~2010) 육성하며모수의유전형질이안정적으로유지되는지에대해서 2 차평가를실시하였다. 그중내한성, 내병성등생육특성이뛰어나고차의기호성에관여하는 TFAA, Teanin 함량이기존도입품종인야부기다보다 1.5 배높은계통을최종적으로선발하여 장원 2 호 라명명하고한국생명공학연구소에종자기탁과특허등록을완료하였다 ( 특허 : ). 본연구결과국내육성차나무품종인 장원 2 호 는기존도입품종들과비교하였을때맛과향의기호성에서우수한특징을나타내었을뿐만아니라피부보습효과도뛰어난것으로밝혀졌다. 이는새로운신품종을활용한기호성이뛰어난다류제품뿐만아니라부가가치가높은화장품원료로서도그가치가뛰어난것을알수있다. * 주저자 : Tel , leems@osullocfarm.com 141

184 PB-12 Hyung Seong Lee, Haeun Jung, Seongbin Hwang, Hyun Uk Kim * Melon (Cucumis melo L.) is an economically important fruit crop because of its productivity in worldwide. However, powdery mildew (PM) diseases, the main factor of reducing crop yields, affect melon farming these days. For this reason, we are developing the molecular makers to detect PM resistance in melon breeding lines. We got 8 PM resistance melon lines, and chose some genes as the candidate of molecular makers. We first chose the Mildew Locus O (MLO) genes that is known as susceptible genes. If they are lost their function by mutation, the melon lines get resistance to PM. We found 14 MLO genes in melon database, and categorized into six clades. Among of them, MLO genes in clade5 are susceptible factor to PM. CmMLO3, 5, and 12 are grouped in clade5. We get their cdna from 8 resistance lines, and analysis the gene expression and sequence. Unfortunately, There is no differences between susceptible and resistance lines. So we found another susceptible genes, named PMR. On the basis of previous researches, PMR4, 5, and 6 are susceptible factor to PM, so we searched the PMR genes in melon, and get cdna from 8 resistance lines. We will identify cdna sequence of these lines, and compare to reference genes. Finding differences between susceptible and resistance lines will guide us to identify where the loss of function mutation is occurred. It can help to develop the PM molecular marker. *Corresponding Author: Tel , hukim64@sejong.ac.kr PB-13 Seokhyung Lee 1, Gyu Tae Park 1, Jagadeesh Sundaramoorthy 1, Jeong-Dong Lee 1, Hak Soo Seo 2, Jong Tae Song 1* 1 2 Soybean [Glycine max (L.) Merr.] is one of the major agricultural crops, which is widely known as a salt sensitive crop and may result in low yield in salt-affected areas. Therefore, it is necessary to develop salt tolerant soybean varieties for increasing yield in salt-affected areas. In a previous study, a dominant gene (GmSALT3) for salt tolerance was identified and nine haplotypes (H1 to H9) were detected among a total of 221 soybean accessions from China. The purpose of the present study is to develop molecular markers for screening salt tolerant and sensitive lines among G. max and G. soja accessions distributed in Korea, Japan and China depending on haplotypes in GmSALT3 gene. Sequence analysis for GmSALT3 gene from a total of 102 lines of G. max and 119 lines of G. soja showed various polymorphisms in coding regions. Depending upon the polymorphisms we identified 37 novel haplotypes and three formerly reported haplotypes (H1, H2, and H5). Among 40 haplotypes indentified, eight haplotypes showed tolerant and 32 haplotypes showed sensitive phenotypes. To confirm whether the polymorphisms detected in coding regions influence the GmSALT3 expression, we performed reverse transcription-polymerase chain reaction. As a result, we detected expression of GmSALT3 in all tolerant and in most of sensitive haplotypes. Therefore, currently we are looking for the other possibilities to explain the relationship between haplotypes and phenotypes. Acknowledgement: This work was supported by Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ ) Rural Development Administration, Republic of Korea. *Corresponding Author: Tel , jtsong68@knu.ac.kr 142

185 PB-14 박기진 *, 박종열, 류시환, 서영호, 최재근, 남궁민, 용우식, 윤석원, 최준근 국내옥수수재배는풋옥수수용도인찰옥수수가중심이되면서그동안연농 1 호 ( 일명대학찰, 1999), 미흑찰 (2004), 찰옥 4 호 (2004), 미백 2 호 (2005), 일미찰 (2005) 등품질이우량한품종들이개발되어현재까지재배되고있다. 하지만이들품종을능가하는품질이우량한품종이지속개발되지못하고있는실정으로삶거나단순가공등부가가치가낮은소비형태로지속하여왔는데, 이제는부가가치를창출할신품종개발이시급하다. 옥수수연구소는 2000 년부터관상용옥수수에서안토시아닌이다량함유된계통을선발하고육종모집단을구성하거나여교잡을실시하여안토시아닌이우량한계통을개발하여왔다. 그동안육종모집단으로부터색소 1 호 (flint, 안토시아닌포엽이용 ), 청춘찰 (waxy, 안토시아닌풋옥수수 ), 색소 2 호 (semi-flint, 안토시아닌알곡이용 ) 품종을개발하는성과를거두었다. 최근에는미백 2 호의종자친과화분친에대하여각각 3 4 회여교잡을통하여다양한색을가진여교잡계통을확보하였으며이를상호교잡하여미백 2 호와상당히유사한다양한색의교잡종을구성하여적응시험을추진하고있다. 특히이들다양한색의교잡종은미백 2 호와품질과수확시기가비슷하여칼라찰옥수수를상품화하는데많은도움이된다. 안토시아닌함량의차이에따라흰색, 분홍색, 갈색, 검정색등으로분리함으로서식가공재료로직접활용할수있는제품이나상품개발이이루어지게되면옥수수의부가가치를높일수계기가될것이다. * 주저자 : Tel , kjp@korea.kr PB-15 김정곤 1*, 박문웅 1, 성병열 1, 최재성 1, 박미자 1 1 국산밀의품질규격화를위한밀품종별용도에따른품질등급기준을마련하기위하여농가산밀원곡과밀가루의품질분석을통하여본연구를수행하였다. 연구결과를요약하면, 조경밀은강력밀가루 ( 제빵용 ) 용으로적합하였는데 1 등급은해당품종이 95% 이상을기준으로, 이종곡물및타품종비율이 5% 이내이어야함 ( 전품종공통사항 ). 조경밀은용도가제빵용이므로단백질의양과질이높을수록유리함. 따라서조경밀 1 등급의단백질함량은 13% 이상, 2 등급은 12% 이상으로설정하였음. 초자율은원맥의내부형질이유리질과같이단단하고투명한상태를말하는것으로초자질이높은밀은단백질함량이높음. 조경밀 1 등급은초자질함량이 75% 이상, 2 등급은 50% 이상으로설정하였음. 원맥 Falling Number 는발아정도를판단할수있는항목으로 FN 값 300 sec 이상은정상이라고판단할수있음. 발아된밀로생산한제빵용밀가루는반죽이끈적이고, 급수량이낮아져완제품품질이떨어지게됨. 참고로, 밀가루의단백질과침전가항목은원맥의단백질함량과정의상관관계를갖는항목이며, 조경밀원맥등급의참고자료로활용할수있음. 금강밀의용도를제빵과제면용으로정했을때준강력수준의단백질함량이바람직함. 금강밀 1 등급의단백질함량은 12.5% 이상, 2 등급은 11.5% 이상으로설정하였음. 금강밀 1 등급은초자질함량이 50% 이상, 2 등급은 25% 이상으로설정하였음. 원맥 Falling Number 는발아정도를판단할수있는항목으로, FN 값 300 sec 이상은정상이라고판단할수있음. 발아된밀로생산한제빵용제면용밀가루는완제품품질을떨어뜨리게함. 해당금강밀은등급기준을설정하기에는샘플수가부족하여과거에분석된자료를감안하여기준을설정하였음수안밀의용도는중력밀가루 ( 제면용 ) 에가까우므로원맥 1 등급의단백질함량을 10% 이상으로설정하였음. 초자율등급기준의설정은불필요함. 원맥 Falling Number 는발아율뿐만아니라전분의호화점도특성을판단할수있고, FN 가높은원맥은밀가루의호화점도가높으며면의식감이향상됨. 따라서수안밀 1 등급의 FN 은 350 sec 이상으로설정하였음. 백중밀과연백밀의용도는박력밀가루제과용으로단백질함량이낮을수록유리함. 원맥 1 등급의단백질함량을 10% 이하, 2 등급은 11% 이하로설정하였음. 초자율등급기준의설정은불필요함. 발아피해를입은원맥은케익과과자품질을떨어뜨리므로 1 등급은 FN 300 sec 이상으로설정하였음. 사사 : 본연구는농림축산식품부연구개발사업 ( 과학기술기반창조농업촉진우선추진과제, 과제번호 : ) 의지원에의해수행하였음. * 주저자 : Tel , kimjgon22@naver.com 143

186 PB-16 강경원 1*, 김백준 1, 조영득 2, 강시용 동양계심비디움은산채에의해수집되거나주로중국및대만에서수입되어상품화되고있는데, 서양계심비디움에비해조직배양에의한묘의생산기간이길고재배가까다로워산업화에어려움이있다. 서양계심비디움은조직배양에의한증식및재배가용이한반면, 대부분일본에서육종되어도입된품종이주를이루고있어관상가치가높은국산품종개발이요망된다. 본연구에서는테이블위에화분재배에적합한식물체가소형이면서, 잎에무늬가있어관상가치가높아소비자가선물용으로수요가높고, 생산농가는작은면적에서대량생산이가능한동양계심비디움의수입을대체할수있는국산품종을육성하여보급하고자하였다. 원품종황용금은잎에황금색의속빛무늬가있는품종이다. 생장점을배양한기내 protocom-like body (PLB) 를한국원자력연구원첨단방사선연구소감마선조사시설에서 30, 50, 70Gy 의선량으로 24 시간조사하였다. 조사한배양체를계대배양하면서배양병내에서잎의변이발생을관찰한결과, 이듬해에 50Gy 조사구에서원품종보다단엽성이면서잎의갓줄무늬녹색이뚜렷한변이체를기내선발하였다. 이변이체를조직배양에의한증식및고정과정을거치고, 온실에서순화및재배과정을거쳐변이특성이고른계통을최종선발하였다. 선발계통의초장은 6.5cm 로원품종 16cm 에비교하여아주작아진왜성변이체이다. 선발계통의잎폭도 0.7cm 로원품종 0.9cm 에비교하여작고, 잎두께도두꺼워졌다. 그리고, 잎의가장자리에남아있는녹색의줄무늬도원품종에비교하여두껍고진하여뚜렷하다. 따라서, 선발된변이계통은초형이작은왜성으로조직배양묘의순화및순환재배가용이하고단위면적당생산효율도높은장점이있다. 또한, 원품종속빛무늬보다단엽성이며잎의녹색갓줄무늬도뚜렷하여관상성및소비자선호도가높을것으로사료되어, 앞으로품종보호권등록및대량생산상품화를추진할예정이다. * 주저자 : Tel , dawin7@hanmail.net PB-17 김정주 1*, 신운철 2, 정재혁 3, 강위금 중산간지역에서관행적인조기이앙, 질소질비료과용등으로쌀의외관품위와밥맛이떨어지는문제가발생하고있어이앙시기및적정질소질비료살포량을설정하여적기에이앙하고균형시비를함으로써조생종쌀의품질향상에기여할수있다 년에육성된중산간지적응조생종벼품종, 설레미 는수량성이높고도정특성이우수하여중산간지역에서재배면적이점차증가하고있는추세이다. 설레미 는 2 주간격 (5 월 6 일, 5 월 20 일, 6 월 5 일, 6 월 20 일 ) 으로이앙하여주요농업적특성및완전미수량등을조사한결과이앙적기를 6 월 5 일로설정한바있다. 질소질비료살포량 4 수준 (0, 5, 10, 15kg/10a) 과재식밀도 4 수준 (60, 70, 80, 90 주 /3.3m 2 ) 으을처리하여 2015 년과 2016 년, 2 개년간주요농업적특성및완전미수량등을조사한결과는다음과같다 년평균출수기는연차간차이는있으나재식밀도및질소시비량에관계없이 8 월 7~8 일로비슷하였고, 질소시비량이많을수록간장과수장이커지는경향이었음 2. 질소시비량 15kg/10a 시험구는재식밀도와상관없이수당립수와천립중의증가로대부분도복되었음 3. 질소시비량이증가할수록재식밀도별m2당수수, 수당립수는증가하는경향을보였고, 등숙비율은감소하는경향을나타냈음 4. 백미수량은질소시비량이많을수록증가하여질소시비량 15kg/10a 수준에서 674~688kg/10a 범위로높게나타났음 5. 질소시비량이많을수록단백질함량은높아지는경향을보였고완전미비율은낮아지는경향이었으며이에따라완전미수량도낮아지는경향이었음. 따라서중산간지적응조생종벼품종 설레미 를재배할때재식밀도는완전미수량을고려하여 80~90 주 /3.3m 2 수준이적정할것으로보인다. 또한, 질소질비료는 15kg/10a 을살포하였을때도복에따른수량및미질저하의우려가있을뿐만아니라등숙률의저하로완전미수량이감소하므로적정질소질비료살포량은쌀의외관품위및완전미수량을고려했을때본답의양분상태에따라도복의위험을고려하여 10kg/10a 내외가적절하다. 사사 : 본연구는농촌진흥청아젠다사업 ( 과제번호 : PJ ) 의지원에의해수행되었음 * 주저자 : Tel , jjkim74@korea.kr 144

187 PB-18 박현수 1*, 김기영 2, 백만기 1, 조영찬 1, 김보경 1, 남정권 1, 고재권 1, 신운철 1, 김우재 1, 고종철 1, 김정주 1, 김현순 1, 이점호 2, 정종민 1, 강경호 1, 정지웅 1, 전재범 1, 이영복 신진백 은벼흰잎마름병저항성유전자를집적한고품질중만생벼품종으로기후변화에의한고병원성신규레이스의분화로인한피해확산에대비하고자농촌진흥청국립식량과학원에서개발하였다. 2007/2008 년동계에벼흰잎마름병저항성유전자 Xa3 와 xa5 를가지고있는 익산 493 호 ( 이후 진백 으로명명 ) 를모본으로하고, 최고품질품종이면서 Xa3 를보유하고있는 호품 에야생벼에서유래한 Xa21 을도입한자포니카형근동질계통 HR 을교배한 F 1 을부본으로하여삼원교배하였다. 집단육종법과계통육종법을이용하여벼흰잎마름병균에대한생물검정과함께저항성유전자와연관된분자표지를이용한 MAS 로세개의저항성유전자 Xa3+xa5+Xa21 이집적된저항성계통을선발하였으며, 표현형이우수한계통에대해서생산력검정시험을수행하였다. 출수기가남평보다늦은중만생종으로벼흰잎마름병균에광범위고도저항성을나타내며도열병과줄무늬잎마름병에도강한복합내병성으로, 쌀의외관품위와밥맛이양호한단간내도복계통 HR27814-B-B 을선발하여 익산 575 호 로계통명을부여하였다 년 3 년간실시된지역적응성검정시험결과 익산 575 호 는벼흰잎마름병에대한저항성이증진된복합내병성계통으로밥맛이양호하고도정특성이우수한점이인정되어직무육성신품종선정심의위원회에서품종명 신진백 으로명명되었다. 신진백 은우리나라벼품종중에최초로 Xa21 을도입하여세개의벼흰잎마름병저항성유전자 Xa3+xa5+Xa21 가집적되어기존의저항성품종들에비해벼흰잎마름병에대한저항성이향상된복합내병성실용품종이다. 또한외관품위와밥맛등이우수한고품질밥쌀용품종으로흰잎마름병발병상습지및친환경재배단지에활용할수있어안전하고밥맛좋은쌀생산에기여할것으로기대된다. * 주저자 : Tel , mayoe@korea.kr PB-19 Shi Jun Ma, Ju Kyong Lee * To better understand the morphological variation for Perilla crop in north areas of China, we studied the morphological variation of 87 Perilla accessions by examining 7 quantitative and 10 qualitative characters. As a result, morphological variation determined that between cultivated var. frutescens and cultivated var. crispa, showed significant morphological differences in 5 quantitative traits including effective number of branches (QN2), number of internodes (QN3), number of branches (QN4), length of the largest inflorescence (QN5), and days from seedling to flowering (QN7). However, two quantitative traits plant height and number of floret of the largest inflorescence did not show significant differences between cultivated var. frutescens and cultivated var. crispa. In principal components analysis (PCAs), five quantitative characters [plant height (QN1), effective number of branches (QN2), number of internodes (QN3), number of branches (QN4), and days from seedling to flowering (QN7)] and 6 qualitative characters [fragrance of plant (QL1), color of reverse side of leaf (QL3), degree of pubescence (QL5), color of flower (QL6), shape of leaf (QL7), and hardness of seed (QL10)] contributed to the positive direction on the first axis. The other quantitative and qualitative characters contributed to the negative direction on the first axis. Most accessions of cultivated var. frutescens and cultivated var. crispa were clearly separated by the first axis. In addition, most accessions of cultivated var. frutescens from high latitude and middle latitude areas were clearly separated by the first axis, except for several accessions. These findings in this study will provide useful information for understanding the morphological variation of Perilla crop according to the geographical distribution in high latitude and middle latitude areas of China. *Corresponding Author: Tel , jukyonglee@kangwon.ac.kr 145

188 PB-20 윤영미 1, 김지은 2, 조성우 2, 강천식 1, 정영근 1, 박철수 2* 1 2 국내식품회사에서중화면제조에이용되는국내기계와연구기관에서주로사용하는일본기계를이용하여중화면을제조하여식미특성을비교분석하였다. 국수기계의롤러의크기와무게의차이로국내기계는일본기계에비해서가수량이 5% 높고, 반죽휴지기간이 30 분짧았다. 국내품종에서는국수면대두께는단백질함량과믹소그래프의가수량에서제조기계와상관없이정의상관을보였다. 단백질함량이다른 3 품종의면대두께는두기계모두에서단백질함량, 침전가및믹소그래프반죽시간과정의상관을나타내었다. 부분찰밀과찰밀의면대밝기는국내기계에서만아밀로스함량에정의상관을보였다. 삶은국수특성을살펴보면, 국내기계가일본기계에비해서경도가높고탄성은낮았으며점성은차이가없었다. 단백질함량이다른 3 품종에서는기계종류와상관없이단백질함량이증가할수록삶은국수의경도는증가하였지만, 탄성과점성은상관이없었다. 부분찰밀과찰밀에서는두기계에서모두아밀로스함량과 setback 은삶은국수의경도, 점도, 탄성과정의상관을보였고, 손상전분과 breakdown 은부의상관을보였다. * 주저자 : Tel , pcs89@jbnu.ac.kr PB-21 김정주 1*, 신운철 2, 강위금 1, 조현숙 3, 양창인 4, 강신구 벼는생육기간중온도및일사조건에의해벼생육, 수량및품질에크게영향을받는다. 기후변화에따른온도상승으로쌀수량감소뿐만아니라쌀의배백, 심복백발생및동할미증가등쌀품질이저하되고온도변화, 일조의다소및강우일수증감등의변화에따라벼생육특성의연차간차이를나타낸다. 따라서중산간지역인경북상주시화서면 ( 해발 285m) 에소재하고있는국립식량과학원상주출장소시험연구포장에서 2014 년 ~2016 년, 3 개년간오대벼를재배하여벼재배군락주위의미세온도변화와벼생육양상및양분관련특성의연관성을추정하기위하여벼재배군락주위의온도변화, 작물학적특성및벼생육시기별식물체내양분변화를조사하였다. 작물학적특성및식물체내양분 (N, P, K) 은이앙기, 분얼초기, 분얼성기, 유수형성기, 출수기, 수확기로나누어생육단계별로조사하였고온도는대기와군락내온도, 수온, 지온을 5 월 21 일부터 9 월 20 일까지 3 개년간조사하였다. 대기및지온의적산온도는 2016 년도에높게유지되었고군락내온도및수온의누적온도는 2014 년도에낮게유지되었다. 출수기전까지대기의연차간온도변화는 2015 년에낮게유지되었고지온의변화는 2016 년도에높게유지되었으며수온및군락내온도변화는큰차이를보이지않았다. 벼키의변화는 2014 년, 2015 년, 2016 년순으로증가속도가빨랐고, 건물생산량의변화는 2014 년도에증가속도가빨랐다. 대기의온도와지온이높게유지된 2016 년도에벼생육이진행됨에따른식물체내양분함량의변화가적었다. 따라서식물체내양분의흡수, 이동에따른벼생육및건물생산은대기의온도및지온의영향이클것으로보인다. 벼재배지역의미세환경변화에의해벼생육반응및양분의흡수, 이동에영향을주므로재배지역의미세온도변화, 양분의흡수및이동과벼생육양상의연관성을추정하기위해서는각각의데이터를장기적으로확보하여분석할필요가있다. 사사 : 본연구는농촌진흥청아젠다사업 ( 과제번호 : PJ ) 의지원에의해수행되었음 * 주저자 : Tel , jjkim74@korea.kr 146

189 PB-22 Jin-A Kim, Sung-Jin Han, Sang-Woo Choi, Dong-Hui Kang, Su-Jin Lee, Jong-Il Chung * Soybean [Glycine max (L.) Merr.] is an important source of protein and oil for humans and animals. Sucrose, raffinose, and stachyose are major soluble sugars present in mature soybean seeds. Stachyose is the most nutritionally undesirable in the soybean, because it causes flatulence and diarrhoea. Soybean cultivars with genetically low levels of stachyose enhance the utilization of soybean in food as well as feed uses. The objective of this research is to obtain the information on selection possibility of soybean lines with low stachyose content using DNA marker based on RS2(rs2) gene. Genetic population was developed from the crosses of (low stachyose content) and PI (normal stachyose content) parents. Twenty F 2 plants of RS2_ genotype and twenty F 2 plants of rs2rs2 genotype from each populations were harvested. Content of stachyose was detected by HPLC. Stachyose contents (g/kg) of and PI were 3.7, 23.7, respectively. Stachyose content 20 F 2 plants with RS2_ genotype was and stachyose content 20 F 2 plants with rs2rs2 genotype was Mean difference between RS2_ genotype and rs2rs2 genotype was highly significant. From this results, selection of genetic lines with low stachyose content by DNA marker based on RS2(rs2) gene will be possible. *Corresponding Author: Tel , jongil@gnu.ac.kr PB-23 Su-Jin Lee, Sung-Jin Han, Sang-Woo Choi, Dong-Hui Kang, Jin-A Kim, Jong-Il Chung * Soybean [Glycine max (L.) Merr.] seed is one of the major food sources for protein, oil, carbohydrates, isoflavones, and many other nutrients to humans and animals. Raffinose and stachyose are main antinutritional factors in soybean seed. Both raffinose and stachyose are carbohydrates, belonging to the raffinose family of oligosaccharides (RFOs). RFOs are not readily digested in humans and cause flatulence or diarrhea. Genetic reduction of raffinose and stachyose content in mature soybean seed will improve the nutritional value of soybean. The objective of this research was to evaluate agronomic traits with 10 F 6 strains selected from breeding populations derived from the cross by seven parents. Content of raffinose and stachyose in mature seed was detected by HPLC. Agronomic traits such as flower color, flowering date, harvesting date, lodging, plant height, seed coat color, hilum color, 100 seed weight, and yield were evaluated. Total 10 intermediate parents with low raffinose and stachyose content were improved intermediate parent has small seed size. Six intermediate parents (15A1, 15D1, RS-5, RS-33, RS-64 and RS-70) have medium seed size and two intermediate parents (14G20 and RS-21) have large seed size. RS-21 intermediate parent has black seed coat and green cotyledon. Four intermediate parents (883-1, 14G20, RS-5 and RS-21) have elite agronomic traits. New intermediate parents developed through this study will be used to improve new soybean cultivars with low raffinose and stachyose content. *Corresponding Author: Tel , jongil@gnu.ac.kr 147

190 PB-24 Jong-Min Jeong 1*, Woon-chul Shin 1, Man-Kee Baek 1, Hyun-Su Park 1, Chun-Song Kim 1, Jeong-Kwon Nam 1, Young-Chan Cho 1 1 Stable germination and seedling establishment is an important factors for the success of direct seeding cultivation. In order to develop rice cultivars adaptable to wet direct seeding, it is necessary to investigate genetic resources, providing basic knowledge to allow the introduction of genes involved in Anoxia germination (AG) ability from accessions into elite cultivars. 139 recombinant inbred lines derived from Tongil-type rice cultivar Milyang 23 and Japonica rice cultivar Gihobyeo were used a mapping population. Evaluation of AG was performed at phytotron in NICS (Jeon-ju, Korea) under temperature variation of 20±5 C per day. After sowing, 10cm depth of water was irrigated and the germination rate was measured at 15 days after sowing. In the QTL analysis using composite interval mapping, we identified two putative QTLs associated to AG tolerance at germination stages. These QTLs located on chromosome 2 and explained 9.43% and 14.75% of the phenotypic variance for AG, respectively. We would be to confirm the detected QTLs through fine mapping and detection of candidate gene. This result would be helpful to facilitate MAS system for improvement of AG resistance in rice variety breeding program for wet direct seeding. *Corresponding Author: Tel , jjm0820@korea.kr PB-25 Jong-Min Jeong 1*, Woon-chul Shin 1, Man-Kee Baek 1, Hyun-Su Park 1, Chun-Song Kim 1, Jeong-Kwon Nam 1, Young-Chan Cho 1 1 In order to secure competitiveness of our rice and increase consumption of rice, it is necessary to develop high quality new varieties that have our own unique taste to meet consumers' preferences. In this study, We carried out a genome wide association study (GWAS) for 10 traits related to the eating and cooking qualities using 96 Korean japonica rice germplsam. The 96 Korean japonica germplsam consist of 24 varieties, 26 landraces, 22 weedy rices, 19 breeding lines and five Japanese japonica varieties. The traits for eating and cooking quality, amylose content (AC), protein content (PC), alkali digestion value (ADV), glossiness of cooked rice (GCR) and 6 parameters for pasting properties from the rapid viscosity analyzer (RVA) were measured. In the GWAS analysis, a total of 3 marker-trait associations at P< for the break down (BD) were identified on chromosomes 1 and 6. Among them, 2 SNP makers associated BD, ab and ab on chromosome 6 located in the interval of wx loci, and explained 48.4% and 47.4% of the total variation in BD, respectively. These SNP marker closely linked to the wx gene could be applied to rice eating quality improvement with marker-assisted selection. *Corresponding Author: Tel , jjm0820@korea. kr 148

191 PB-26 Hyo-suk Kim 1,3, Sungyul Chang 2*, Youngchul Chung 3, Hyeong Seok Kim 2 and Jae Hun Kim Selecting and implementing non-destructive methods to study complex biological system was active research topic in both plant and animal kingdom, since it could measure the complex system continuously in vivo. Recently, phenomic matrix that only contained visible morphological spectrum images (visible phenotypic marker) enhanced our understanding of complex biological traits greatly. However, visible spectrum data alone was not enough to study complex functional food trait. Hence, we implemented hyperspectral (multiple spectrum ranges) imaging technique to detect anthocyanin presence in multiple bok choy cultivars. The results showed that the method accurately detected anthocyanin concentration compared to traditional destructive chemical assays (HPLC). Furthermore, the method was sensitive enough to detect anthocyanin expression patterns influenced by environment factors in an identical bok choy cultivar. The fast and reliable method coupled with multi-omics data might obtain the complete picture of complex biological trait. *Corresponding Author: Tel schang8@kist.re.kr PB-27 김영진 *, 정종성, 황태영, 김지혜, 김원호 본연구는충남천안에서수행하였으며, 옥수수품종은조사료용인 광평옥 을이용하였다. 파종은 2016 년 4 월 25 일과 5 월 12 일에 75x16cm 재식거리로ha당 40 kg의종자를점파하였다. 질소시비량은 200kg/ha 과 220kg/ha 을처리하였으며, 기비는 50%, 추비는생육 7 8 엽기에 50% 로나누어시용하였다. 수수 수단그라스교잡종품종은출수형인 SX-17 을이용하였다. 파종은 2016 년 4 월 20 일과 5 월 9 일에주간거리 50cm 로ha당 40 kg의종자를세조파하였으며, 각시험구면적은 12 m2 (3 4m) 로하였다. 질소시비량은 250kg/ha 과 300kg/ha 을처리하였으며, 기비는 40%, 생육기에 30%, 1 차예취후에 30% 로나누어시용하였다. 옥수수의사료가치 (TDN) 는 4 월 25 일파종에서 71.75% 로가장높았으며, 조단백질은 5 월 12 일파종에서 9.26% 로가장높았다. 반면소화율은 5 월 12 일파종에서 77.05% 로가장높은수치를보였다. 질소 200kg/ha 처리가 220kg/ha 처리보다사료가치 (TDN) 가높고, 소화율도좋았으나, 조단백질함량은약간적은경향이었다. 또한질소분시는기비 : 추비 100:0% 처리가 50:50% 처리보다사료가치 (TDN) 가높고소화율도좋으며조단백질함량도높은경향이었다. 수수 수단그라스교잡종의사료가치 (TDN) 와조단백질함량은 1 차예취시에는 4 월 20 일파종에서가장높았으며, 2 차예취시에는 5 월 9 일파종에서가장높았다. 질소 300kg/ha 처리에서의사료가치 (TDN) 는 250kg/ha 보다 1 차예취시높았으며, 소화율도양호했다. 질소분시사료가치 (TDN) 는 2 차예취시 40:30:30% 가 50:50% 에서보다양호했으며조단백질함량도높았다. * 주저자 : Tel , yjikim@korea.kr 149

192 PB-28 Jung-Woo Lee 1*, Jang-Uk Kim 1, Ick-Hyun Jo 1, Ji-Eun Hong 1, Kyong-Hwan Bang 1, Kee-Choon Park 1, Young-Chang Kim Korean ginseng is propagated by seeds, which are required 3-4 years to produce mature seeds from their mother plants. It is very important to improve germination rate since ginseng seeds take a long time to be set and the price is high. Also it is required to establish the early breaking dormancy techniques in order to reduce the breeding cycle. In this study, we conducted to investigate the effect of kinetin and/or gibberellin on seed germination in Panax ginseng C.A. Meyer. Ginseng berries were harvested when they are mature completely from a research field of the Department of Ginseng research, National Institute of Horticultural and Herbal Science, RDA Eumseong, Korea in July Dehiscent seeds for germination test were immersed in a solution containing 50 mg/l or 100mg/L GA 3 for 24 hours, respectively at room temperature. Also, in order to confirm the combination effect of kinetin and GA 3, the seeds were immersed in a solution containing 50mg/L kinetin+100mg/l GA 3. GA 3 treatment has a little effect on breaking dormancy and germination, but could not be substituted for chilling requirement in P. ginseng due to characteristic of deep physiological dormancy. Kinetin has a significant effect on breaking dormancy and germination although a short period of cold treatment was required. Combination of GA 3 and kinetin treatments was more effective than each hormone treatments. Acknowledgement: This work was carried out with the support of the Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ ) of the Rural Development Administration, Republic of Korea *Presenter Corresponding Author: Tel , enzymer@korea.kr PB-29 Jung-Woo Lee 1*, Jang-Uk Kim 1, Ick-Hyun Jo 1, Kyong-Hwan Bang 1, Kee-Choon Park 1, Young-Chang Kim The ginseng seeds separated from sarcocarps are surrounded by hard and impermeable seed coat acting a mechanical inhibitor. It probably takes about 21 months to achieve germination owing to delayed embryo development when the seeds detached naturally from their mother plants. In order to shorten the period, the seeds are placed in wet sand for over 90 days with periodic watering at known as warm stratification or dehiscence. In completely dehisced seed, zygotic embryo is still immature and required for additive embryo development to overcome morphological dormancy. In this study, we conducted to investigate the effects of stratification periods, kinetin and/or gibberellin treatment on zygotic embryo development in Panax ginseng C.A. Meyer. Dehiscent seeds for measuring embryo development were immersed in a solution containing 50 mg/l or 100mg/L GA 3 for 24 hours, respectively at room temperature. Embryo and endosperm length were measured at intervals of 15 days for 90 days using light microscope after cutting ten seeds in half and then embryo to endosperm ratio was calculated. The embryo grew gradually as the cold stratification period increased, but it was rapidly increased between 30 and 60 days. The germination characteristics of seeds treated by kinetin were excellent when compared with GA 3 treatment and control during the same treatment period. The maximum embryo to endosperm ratio (0.99) observed at GA 3 +kinetin treatments with 90 days cold stratification, but there is no difference with kinetin treatment. Acknowledgement: This work was carried out with the support of the Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ ) of the Rural Development Administration, Republic of Korea *Corresponding Author: Tel , enzymer@korea.kr 150

193 PB-30 Ye Rin Lee 1, Myeong Hyeon Nam 2, Won Suk Jang 2, Jin Hoon Lee 1, Jundae Lee 1* 1 2 Crown rot caused by Phytophthora cactorum is a serious disease damaging strawberry (Fragaria x ananassa Duch.) in worldwide. In this study, we aimed to identify resistant strawberry cultivars against P. cactorum through bioassay. A total of 104 strawberry cultivars including 'Sulhyang', 'Santa' and 'Akihime' were inoculated with an isolate, P. cactorum PC151111, obtained from the Nonsan Strawberry Experiment Station. The zoospore suspension was diluted in autoclaved sterilized water to approximately spores ml -1. The inoculated strawberry plants were incubated at 25±3 C and 16h/8h (light/dark), and the resistance was evaluated through observation of symptom development for 3 weeks. Disease index was scored on a scale from 0 to 4. In result, the disease index scores varied from 0 to 2.7 and the 14 cultivars, Sinyeobong, Yeohong, Sulhong, Sukyeong, Santa, Gwanha, Kamarosa, Festival, Flamengo, Okmea, J&Q s early, Goryeong, Gumsil, and Diamante, were highly resistant to P. cactorum. These resistant resources will be useful for the genetic analysis and breeding of strawberry resistant to crown rot. *Corresponding Author: Tel , ajfall@jbnu.ac.kr PB-31 박석만 1*, 고상욱 2, 윤수현 1, 박재호 1, 김민주 1, 이동훈 3, 최영훈 현재제주지역에서재배되고있는감귤류는 85% 이상이노지온주밀감이며나머지가시설에서재배되고있는만감류품종이다 년대말부지화 ( 상품명 : 한라봉 ) 가본격적으로재배되기시작하면서제주지역의만감류재배면적은급격하게늘어나게되었다. 만감류는일반적으로온주밀감과구분되어해를넘겨수확되고온주밀감보다과피가두꺼우며교잡을통해육성된품종들을말한다. 그러나근래에들어육종기술의발달로과거에는교잡이힘들었던온주밀감과다양한품종들과의교잡품종이육성되면서만감류의특성을가지면서도연내에수확되는품종들이육성되고있다. 현재제주에서재배되고있는연내수확형만감류는일본에서육성된 하레히메 와 에히메과시제 28 호 ( 상품명 : 황금향 ) 가있으며 에히메과시제 28 호 는재배면적이급증하고있다. 그러나 에히메과시제 28 호 는과피벗김이어렵고특유의향을보유하고있어소비자들의평가에있어호불호가분명한품종이다. 이에따라본연구는 에히메과시제 28 호 의단점을극복하여소비자들의기호를보다충족시키는품종을육성하고자수행하였다. 윈터프린스는 2007 년 하레히메 를모본으로 태전병감 을교배하여육성된교배실생으로 2008 년실생을탱자에접목하여 2014 년 1 차선발을실시하여 2016 년에최종선발하여올해 5 월품종보호출원을실시하였다. 과실특성은 10 월하순부터착색이시작되어 12 월상순에완전착색되며과실은옅은오렌지색이다. 과실크기는평균 150g 내외이며성숙기당도가 12 Brix 정도이고, 산함량은 0.8% 로당산비가높고과즙이많고과육이부드럽다. 나무의특징은부본인병감과유사하여수세가강하고가시가발생하지않는다. 또한자가불화합성으로종자가발생하지않는다. 윈터프린스의경우 에히메과시제 28 호 의가장큰단점인과피벗김에있어과피가두껍고강도가있어온주밀감과같이매우쉽게벗겨낼수있다. * 교신저자 : Tel , babau2000@korea.kr 151

194 PB-32 In-Sok Lee *, Chan-Ho Kang, Ki-Kwon Lee Kenaf (Hibscus cannabinus L.) is a real jack of all trades in terms of using such as fiber, forage and fuel. However, kenaf seeds are reported to have poor physiological quality limiting biomass productivity. In this study, the effects of different concentration of three priming sources such as Hydro, KNO 3 and PEG on kenaf seed have been investigated to determine the optimal conditions for surging germination. A variety was Jangdae released from KAERI. The germination rates, MGD (mean germination days) and dry weight of the treated sample seed were compared with those of the untreated seed germinated undernon-stress and salt stress conditions. First, there was significant difference in germination rate of kenaf compared non-stress and salt stress (0.1, 0.2, 0.3, 0.4 and 05% NaCl) in 24 hours. However, we could not find some difference of germination in 62 hours. It was possible for us to find significant difference in both shoot and root growth degree after 5 days. And, there was no difference in dry weight regardless of stress treatment. RD50 (salt concentration of causing 50% growth reduction) was 0.2~0.3% NaCl based on the shoot and root growth degree. The seeds were treated with Hydro, KNO 3 and PEG. It was found that germination of the treated kenaf seeds was sharply increased compared with that of the untreated seeds in 12 hours under non-stress (distilled water) and 0.3% salt stress conditions. As to MGD, a significant difference was shown in comparison with the treated and untreated kenaf seeds under two conditions. In dry weight (mg/10 plants), sample s dry weight treated with Hydro and KNO 3 was increased under non-stress condition compared with that of the untreated one. But, we found an opposite result under stress condition. The results showed significant effects of priming at 10% PEG concentration under non-stress. Up to 20% PEG, a dry weight of plants cultivated under 0.3% salt concentration was increased, since then, decreased. Three priming techniques proved to be efficient in reducing the germination period. The results suggest that priming with Hydro, KNO 3 and PEG optimized seed germination and vigor and is therefore recommended for kenaf seed invigoration before planting. *Corresponding Author: Tel , bioplant325@korea.kr PB-33 남정권 1, 박현수 1, 김기영 3, 김보경 1, 조영찬 1, 고재권 1, 김현순 1, 백만기 1, 김정주 2, 김우재 1, 신운철 1, 고종철 1, 이점호 3, 정종민 1, 정지웅 1, 강경호 1, 이영복 우리나라벼재배면적중중만생품종의재배면적이약 85% 를점유하고있어수확기홍수출하에따른쌀값하락현상이매년반복되고있는실정이다. 밥맛이좋고병해에저항성이면서수량성이높은조생품종을개발하여재배면적이확대되면중만생종집중출하에따른쌀값하락방지, 콤바인등농기계작업시기분산등의효과를거둘수있다. 국립식량과학원은 2006 년하계에조생종인운봉 37 호를모본으로하고진부와운광이교배된 F1 을부본으로인공교배를하였으며, F3 이후는계통육종법에따라우량계통을선발하였다. 생산력검정시험에서조생, 다수성이면서흰잎마름병과줄무늬잎마름병에강한우량계통 HR 에 익산 570 호 로계통명을부여하였다. 익산 570 호 는 년까지 3 년간지역적응시험결과, 그우수성이인정되어 조일 로품종명을부여하였다. 평야지조기재배에서 조일 의출수기는 7 월 16 일로 조평 과비슷한조생종이며, 간장은 72cm 로단간이며내도복성이다. 조일 은벼흰잎마름병 (K1 K3) 과줄무늬잎마름병에저항성이나도열병에는중저항성이다. 조일 의수발아율은 10.9% 로조평의 25.2% 에비해낮은편이다. 조일 의쌀수량은 년실시한지역적응시험보통기보비재배에서 5.39MT/ha 로 조평 보다 3% 증수되며쌀알은맑고심복백이거의없다. 조일 의적응지역은경기이남평야지대와소득작물후작지이다. 조일 은평야지특히남부평야지에서추석전조기출하용으로적합한조생종이다. 주저자 : Tel , namjk725@korea.kr 152

195 PB-34 남정권 1*, 신운철 1, 박현수 1, 정종민 1, 김춘송 1, 백만기 1, 조영찬 1, 고재권 1, 정희채 1, 김보경 1 1 쌀시범사업연계산업용 RPC 도정특성및가공적성평가를위하여벼최고품질품종인삼광, 하이하미, 수광등 13 품종을저온 (15 ) 과상온에서 6 개월및 9 개월저장후품종별밥의윤기치와도정특성을분석하였다. 저장전시료의밥의윤기치 (Toyo 식미치 ) 는삼광 74.6, 운광 75.5, 하이아미 81.2, 영호진미 77.9, 수광 76.0, 해품 73.8 이었다. 저온 (15 ) 과상온에서 6 개월간저장한시료들의밥의윤기치는 로저장온도간에는차이를거의보이지않았다. 저장후 9 개월에서는저장전의식미치에비하여 정도낮았다. 특히, 조생종인운광과해담쌀의식미치저하는중만생종인삼광, 수광, 칠보의밥의윤기치저하폭이컷고, 상온저장에서저온저장보다감소폭이더컸다. 도정율은저장기간이길어질수록동할미발생증가하여도정율이낮아지는경향이었다. 특히상온저장에서저온저장의도정율보다 % 정도낮은경향을보였다. 이상의결과에서조생종은저장기간이길고, 저장온도가높아지면중만생종보다밥맛저하가크므로기온이상승하기전에소비를하는것이유리하다. 또한 RPC 저장시설부족으로벼가야적되는경우가많은데, 이는밥맛과도정율을저하시키는원인으로품질유지를위한품종별최적의저장조건을산업용 RPC 에제시하여쌀소비촉진에기여하고자한다. * 주저자 : Tel , namjk725@korea.kr PB-35 원용재 1*, 안억근 1, 장재기 6, 이상복 2, 이정희 4, 정국현 1, 김명기 1, 양창인 3, 하운구 1, 현웅조 1, 정응기 6, 조영찬 4, 오세관 1, 윤미라 1, 김보경 4, 김병주 쌀소비를높일수있는방안으로쌀가루산업활성화에적합한건식제분전용신품종 한가루 를개발하였다. 건식쌀가루전용벼 한가루 는 2007 년하계에중만생종이면서현미천립중이 34.8g 인대립으로쌀튀김성과현미튀김정립률이좋은 대립벼 1 호 를모본으로, 밥맛이우수한 일품 의돌연변이로뽀얀멥쌀특성을가져양조용으로산업화에성공한 설갱 을부본으로교배하여술을담글때원료곡생산성을높일수있는대립과뽀얀멥쌀의특성을조합하여국내최초로건식쌀가루전용으로개발된품종이다. 한가루 의이삭패는시기는 8 월 23 일로중만생종이다. 벼키는 79 cm로 화성 보다작고, 현미천립중은 32.9g 으로 화성 보다 1.5 배정도무거우며, 쌀수량은 542kg/10a 이다. 쓰러짐에강하지만병해충에약하고, 수발아에중정도의반응을보여재배적응지역은중부평야및호남평야북부지역이다. 한가루 는쌀알의굳기가연질이면서배유내전분구조가밀과같이둥글어건식쌀가루의입자는평균 60 ~ 70 μm로작고, 손상전분이 6.4% 로낮아쌀가루용쌀로적합하다. 한가루 로맥주를만들었을때 설갱 보다환원당함량이높고쓴맛이낮으며목넘김이부드러워양조용에도적합한품종으로확인되었다. 사사 : 본연구는농촌진흥청연구사업 ( 세부과제명 : 중부지역적응초다수성쌀가루전용품종육성, 세부과제번호 : PJ ) 의지원에의해이루어진것임. * 주저자 : Tel , yjwon@korea.kr 153

196 PB-36 Min-kyu Lee 1, Jaihyunk Ryu 1, Dong-Gun Kim 1, Jung Min Kim 1, Jin-Baek Kim 1, Si-Yong Kang 1, Bo-Keun Ha 2, Soon-Jae Kwon 1* 1 2 Faba bean (Vicia faba L.) is an important grain legume extensively grown as a spring crop to avoid freezing damage. However, the expected benefits in the harvest time and grain yields prefer autumn sowing to spring sowing faba bean. Recently, oversupply of rice was issue in South Korea, since high grain yield and developed farming system. The winter faba bean is going to fill as economic crop the time between harvest and transplant of rice field. Plastic mulching provide many positive advantages in crop cultivation such as earlier and higher overall yields, reduced evaporation, and fewer weed problems. The purpose of this study is to compare the difference in growth between PE film mulching field and non-mulching filed in 256 individual lines of faba bean. Total 50 seeds were sown in PE film mulching field (22 seeds) and non-mulching field (28 seeds) at Korea Advanced Radiation Technology Institute in Jeongup, in middle Oct, Of the total 256 lines, zero percentage of survival rate was 63 lines in plastic mulching field and 78 lines in non-mulching field, respectively. Survival plants in PE mulching field had higher plant height than non-mulching field in middle of April, 2016, averagely 10 cm. Flowering started from 29 March, and mulching field was about 2 weeks faster than non-mulching field. In addition, this result was confirmed that the autumn sown faba bean was flowered one month earlier than the spring sown faba bean. These results will be very useful for the cultivation of pulse crop in South Korea, and faba bean will provide great opportunity for mutation breeding. *Corresponding Author: Tel.: , soonjaekwon@kaeri.re.kr PB-37 Dong-Gun Kim 1, Jaihyunk Ryu 1, Min-kyu Lee 1, Jung Min Kim 1, Jin-Baek Kim 1, Chang-Hyu Bae 2, Si-Yong Kang 1 and Soon-Jae Kwon 1* 1 2 Faba bean (Vicia faba L.) is one of important versatile and cool-season pulse crop. As a protein-rich legume, faba bean is well adapting to most climatic areas of North Europe, Australia and Asia country for feed and food. This study was conducted to evaluate the fatty acid composition of 10 elite accessions. The fatty acid was extracted from leaves, green pods and matured seeds in each accessions. The most abundant fatty acids of leaves were a-linolelaidic acid (C18:3)(n=3)(avg 61.9%), followed by Palmitic acid (C16:0)(avg 12.9%) and Linoleic acid (C18:2)(avg 12.7%). PI567883, PI and PI had highest a-linolelaidic acid (63.7%), Palmitic acid (15.2%), Linoleic acid (13.5%) in leave, respectively. In green pods, Linoleic acid (C18:2)(avg 54%) were major fatty acid compound, Palmitic acid (C16:0)(avg 23.3%) and a-linolelaidic acid (C18:3)(n=3)(avg 12.2%) were revealed less than 25%. Among 10 accessions, PI366039, PI and PI had highest Linolelaidic acid (58.9%), Palmitic acid (25.8%), a-linolelaidic acid (20.4%) in green pod, respectively. The major fatty acids of matured seeds were Linolelaidic acid (C18:2)(avg 58.1%), Palmitic acid (C16:0)(avg 19%) and Oleic acid (C18:1)(avg 9.9%). PI252004, PI and PI had highest Linolelaidic acid (63.6%), Palmitic acid (20.8%), Oleic acid (12.7%) in seed, respectively. In this study, we assessed fatty acid of faba bean first adapted in Korea environment and will be useful for functional food using faba bean. *Corresponding Author: Tel , soonjaekwon@kaeri.re.kr 154

197 PB-38 김성업 1*, 이명희 1, 배석복 1, 오은영 1, 오기원 1, 정찬식 2, 여운상 참깨는고온다습한기후에서재배시역병, 시들음병으로인한수량감소피해가크다. 따라서국내에서안정적으로참깨생산을하기위해서는역병과시들음병에강한품종육성이필요하다. 누리 는내병성참깨품종육성을목적으로 1999 년모본 수원 131 호 와부본 양백 (IT229436) 을인공교배하여계통육종법으로육성한품종이다. 고유특성은초형은소분지형이고꼬투리는 3 과성이며꽃과종피는흰색이다. 또한성숙기는 8 월 23 일로표준품종인 고품 보다 2 일느리며경장은 138 cm 로크고주당삭수가 82 개로대등한수준이었다. 병해및도복저항성포장검정결과표준품종인 고품 보다역병, 시들음병, 흰가루병및도복에강한특성을나타내었다. 특히역병유묘검정결과에서저항성으로나타났다. 종실수량은 년 3 개년간전국 7 개지역평균 118kg/10a 으로표준품종인 고품 보다 17% 유의하게높았다. 품질분석결과조지방함량은 49.0%, 단백질함량은 26.1% 으로 고품 보다 3% 낮았으며, 세사민함량은 293mg/100g 으로 고품 보다 19% 낮았다. * 주저자 : Tel , sesameup@korea.kr PB-39 송석보 1*, 고지연 1, 우관식 2, 정태욱 2, 최명은 1, 김정인 1, 고종철 3, 최유미 4, 곽도연 해오롬 은기계화적성이우수하고가공적성이뛰어난팥품종을육성하고자 2008 년하계에국립식량과학원에서단간인밀양 7 호 (SA9709-2B ) 를모본으로하고적색대립인 K204656(Toyomidainagon) 을부본으로인공교배하여계통육종법으로선발한품종이다 ~ 2014 년생산력검정시험에서쓰러짐에강하고수량성이높아 밀양 27 호 로계통명을부여하였다 ~ 2016 년지역적응시험을실시한결과, 가공적성이우수하고내재해성품종으로인정되어 2016 년농작물직무육성신품종선정위원회에서신규등록품종으로결정되었다. 해오롬 의개화일수는 55 일로충주팥보다 9 일정도빠르고생육일수가 92 일로 2 일빠른중만생종이다. 경장은 69 cm이며협당립수는 6.6 개로충주팥보다많고쓰럼짐에강하다. 100 립중은 18.6g 으로맑은적색의종피색을가진대립품종이다. 통팥가공적성이우수하고앙금수율이높아팥가공제품제조시품질면에서유리하다. 수량성은지역적응시험에서평균수량이 2.04MT/ha 로다수성품종이다. 적응지역은강원도산간고랭지를제외한전국팥재배지역에서재배가가능하다. 기계수확이가능한 해오롬 은노동력과생산비절감으로생력화와농가소득증대에기여할것으로기대된다. * 주저자 : Tel , songsb1254@korea.kr 155

198 PB-40 Jung Min Kim 1, Jaihyunk Ryu 1, Min-kyu Lee 1, Dong-Gun Kim 1, Jin-Baek Kim 1, Si-Yong Kang 1, Bo-Keun Ha 2, Soon-Jae Kwon 1* 1 2 Faba bean (Vicia faba L.) has rich L-dopa (3,4-dihydroxyphenyl-L-alanine), the precursor of dopamine, have long been used for the treatment of Parkinson s disease in ancient Eastern Indian ethnotherapeutics. The major objective of this study was to extract L-dopa in Faba bean from 10 accession by ultra performance liquid chromatography (UPLC), with data being gathered from matured seeds, green pod and leaves. The PI contained highest L-dopa content as 15.69±0.10g, whereas PI had lowest L-dopa contents in matured seeds. In green pod, PI included highest L-dopa content (79.58±0.40g), PI had lowest content (16.31±0.02g). In addition, PI contained highest contents (39.82±0.28g) in leaves. The standard deviation of L-dopa in matured seeds, green pod and leaves showed 4.55, and 3.65, respectively. However, green pod (34.73) had higher average of L-dopa contents than leaves (33.91). Ultimately, the new variety of faba bean had high content of L-dopa, should be needed for the functional foods. These results will provide valuable information to assist the potentially environmental friendly natural products of use to medicine and other purpose. *Corresponding Author: Tel , soonjaekwon@kaeri.re.kr PB-41 Tae-Il Park 1*, Yang-Kil Kim 1, Hyoung-Ho Park 1, Ok-Kyu Han 1, Tae-Hwa Song 1, Jong-Chul Park 1, Jong-Ho Park 1, Chon-Sik Kang 1, Young-Jin Oh 2, Kyong-Ho Kim 1, Kyu-Hwan Choi 3, Ki-Hong Hong 4, Hyun-Seok Chae 5, Young-Keun Cheong 1 and Bo-kyeong Kim Oats have the characteristics of a high dry matter yield and good livestock palatability as well as feed value. So it s popular for livestock farming. In addition, being the recent climate warming and diversifying of crop rotation system in rice field of Korea, varieties of oats have been developed for forage use because the imported seeds can be also replaced. A new oat(avena sativa L.) cultivar, 'Dakyeong' was released because of its earliness and high forage yield potential. Heading date was April 30 which was 6 days earlier than that of 'Samhan' as the standard one. Meanwhile, plant length is so high of 122cm, and rate of leaves showed higher of 14.7% than check variety of 10.9%. Over 3 years, forage yield based on fresh and dry matter yield were averaged 45ton ha -1 and 15.7ton ha -1 respectively. Dry matter yield was superior with an increase of 12% more than that of 'Samhan'. Also silage quality is good as the crude protein content was 6.1%, NDF and ADF were 60.0% and 33.9%, respectively, and TDN was 62.1%. TDN yield and silage grade were similar to check one. It was found out Dakyeong would be suitable for areas with above -6 of daily minimum temperature in January in Korean peninsula. *Corresponding Author: Tel , parktl@korea.kr 156

199 PB-42 Tae-Il Park 1*, Yang-Kil Kim 1, Hyoung-Ho Park 1, Ok-Kyu Han 1, Tae-Hwa Song 1, Jong-Chul Park 1, Jong-Ho Park 1, Chon-Sik Kang 1, Young-Jin Oh 2, Kyong-Ho Kim 1, Kyu-Hwan Choi 3, Ki-Hong Hong 4, Hyun-Seok Chae 5, Young-Keun Cheong 1 and Bo-kyeong Kim Oats, which are known as one of the forage crops of Korea, have good livestock palatability and are popular with livestock farmers because of their high dry matter. However, the cultivation of double cropping in the rice field was reluctant due to the late maturing for farmers to plant rice continuously. A new oat(avena sativa L.) cultivar, Hi-early, showed the heading date 10days earlier than that of check variety Samhan. Average fresh and dry matter yield of Hi-early in the regional adaptation yield trial test were similar of 40ton ha -1 and 14ton ha -1, respectively, but it was 20% of 38ton ha -1 and 13ton ha -1 higher than that of Samhan in the paddy field with many tillers and 122cm of plant length, especially. Also, quality was good of silage grade II as the crude protein content was 6.2%, NDF and ADF were 61.9% and 35.3%, respectively, and TDN was 61.0% as the check one. Hi-early would be adapted for areas with above -6 of daily minimum temperature in January in Korean peninsula. *Corresponding Author: Tel , parktl@korea.kr PB-43 Sung Min Han, Hong-Il Choi, Si-Yong Kang, Jin-Baek Kim * Radiation breeding is a powerful tool for creating a new germplasm with various characteristics. Until now, KAERI has produced many rice varieties through radiation breeding techniques. In this study, we tried to compare physiological responses to acute and chronic irradiation, respectively. The rice plants (Oryza sativa L. cv Ilpum) were exposed to 100, 200, 300 Gy doses of acute for 8 hours or chronic for 10 days. The plant height, leaf length and leaf width were decreased with increase of a does than the non-irradiated plants. The tiller number was increased by 100 Gy and 200 Gy chronic irradiation. DNA damage, chlorophyll fluorescence and free radical accumulation were increased. Induced gamma irradiation increases in the contents of H 2 O 2 and malondialdehyde, decreases in the contents of glutathione and ascorbic acid, and increases in the superoxide dismutase and catalase activities. But the ascorbate peroxidase and peroxidase activities were different as gamma-irradiation patterns. Acute and chronic irradiation significantly increased the total phenols contents and phenylalanine ammonia-lyase activity induced under oxidative stress condition. Results suggest that the responses and defense providing reactive oxygen species scavenging systems prompted by irradiation exposure are dependent upon the gamma-ray pattern. *Corresponding Author: Tel , jbkim74@kaeri.re.kr 157

200 PB-44 이진석 1*, 손범영 1, 김정태 1, 배환희 1, 백성범 1, 정태욱 2, 김상곤 1, 김성국 1, 정건호 고당옥 1 호는자식계통 KSE13 을종자친 ( 모본 ) 으로하고 KSE37 을화분친 ( 부본 ) 으로하는단교잡종단옥수수이다. 고당옥 1 호는 2006 년과 2008 년생산력검정시험을거쳐 2014 년 ~2016 년 3 년간전국 5 개지역에서지역적응시험을수행하였으며그우수성이인정되어 2016 년농촌진흥청직무육성신품종으로선정되었다. 고당옥 1 호는 sugary enhancer(se) 유전자를보유한단옥수수로이삭의색은황백색을나타내고이삭형태는중간형을나타낸다. 고당옥 1 호는출사일수는 59 일로표준품종인단옥 3 호와차이가없었고이삭의길이는 18.7cm 로단옥 3 호보다길었으며착립장률은 96% 로단옥 3 호보다우수하였다. 고당옥 1 호의깨씨무늬병, 그을음무늬병, 조명나방등병해충저항성은단옥 3 호와유사하였으며도복은단옥 3 호보다강하였다. 고당옥 1 호의 10a 당이삭수와이삭중은각각 6,530 개 /10a, 1,139kg/10a 로단옥 3 호와유사하였다. 당도는 20.8 Bx 로단옥 3 호보다높았고백립중은 27.4g 으로단옥 3 호보다작은경향을나타내었으며관능평가결과전체기호도가 5.7 로식미가우수하였다. 고당옥 1 호는 se 유전자를보유하고있어일반옥수수, 초당옥수수 (sh, sh2, bt 등 ), 일반단옥수수 (su), 찰옥수수 (wx) 등다른종류의옥수수와함께재배시고유의특성이사라지므로반드시시간적혹은공간적으로격리재배하여야하며품질이우수한단옥수수생산을위하여적기수확이반드시필요하다. 사사 : 본연구는농촌진흥청연구사업 ( 세부과제명 : 식용옥수수우량계통육성시험, 세부과제번호 : PJ ) 의지원에의해이루어진것이다. * 주저자 : Tel , z @korea.kr PB-45 이지윤 1*, 조준현 1, 이종희 2, 손영보 1, 박노봉 3, 한상익 1, 오성환 2, 신동진 1, 송유천 1, 박동수 1, 박병명 1, 오인여 1, 남민희 3, 오명규 한아름 4 호 는가공용원료곡에적합한통일형초다수성품종을만들기위하여, 2007 년하계에직립초형으로수광태세가우수한금강과수량성이높은한아름벼를각각모본과부본으로인공교배를실시하였다 년하계에 F 1 을양성하였고, 2009 년 F 2 집단에서 80 개체를선발한후 2009/2010 년국제미작연구소에서 F 3 를전개하여계통당 3 계통씩선발하였다. 이후 2 년간계통육종법으로세대를진전시켰으며, 2012 년예비선발시험과 2 년간의생산력검정시험을거쳐밀양 295 호의계통명을부여하였다 년부터 2016 년까지 3 년간의지역적응시험결과, 수량성이높고, 줄무늬잎마름병및도열병등에저항성이고수량성등의우수성이인정되어 2016 년농작물직무육성신품종선정위원회에서 한아름 4 호 로명명되었다. 한아름 4 호 는출수기가 8 월 15 일인중만생종이며, 간장은 78 cm로 다산 보다 5 cm크고, 수장은 24 cm로 다산 보다 1 cm작다. 한아름 4 호 는저온발아성과등숙비율이각각 70.0% 와 82.6% 이다. 한아름 4 호 는쌀수량이 797kg/10a 로현재개발된벼품종중에서수량성이가장높아양조용등가공용원료곡으로적합한품종이다. * 주저자 : Tel , minitia@korea.kr 158

201 PB-46 구자환 1*, 한옥규, 안종웅, 권순종 1 북한에서수집된보리유전자원에대해서북방지역에서재배가가능한유전자원을탐색하고자북한원산의보리유전자원 209 점과국내에서육성된동보리 1 호, 올보리, 서둔찰보리, 유연보리를비교품종으로수원 (2016 년 10 월 13 일파종 ), 연천 (2016 년 10 월 7 일파종 ), 진부 (2016 년 10 월 1 일파종 ) 에파종하여월동율및출수기를조사한결과수원지역에서월동중동사한유전자원수는 4 점 (1.9%) 이었고, 연천지역에서월동중동사한유전자원수는 51 점 (24.4%) 이었으며, 진부지역에서월동중동사한유전자원수는 75 점 (32.8%) 이었다. 국내에서육성된비교품종동보리 1 호, 올보리, 서둔찰보리, 유연보리는 3 개지역에서모두월동이가능하였다. 수원지역의유전자원집단평균출수기는 4 월 28 일로나타났으며, 연천지역의유전자원집단평균출수기는수원보다 10.6 일이늦은 5 월 9 일이었으며, 진부지역의유전자원집단평균출수기는수원보다 11.1 일이늦은 5 월 10 일로나타났다. 사사 : 본연구는농촌진흥청어젠다사업 ( 과제번호 : PJ ) 의지원에의해이루어진것임. * 주저자 : Tel , jhku@korea.kr PB-47 Sovetgul Asekova *, Myung Hee Lee, Jung In Kim, Sung Up Kim, Suk Bok Bae, Un Sang Yeo, Eun Young Oh, Ki Won Oh, Do Yeon Kwak Sesame is one of the most ancient oil crop with high nutritional value beneficial to human health. It is widely grown in tropical, subtropical, and southern temperate regions of the world. Various types of molecular markers including RAPD, AFLP, EST-SSRs, ISSRs, SRAP were developed and used to detect genetic diversity for sesame germplasm. There is a limited study associated with genetic diversity of Korean sesame germplasm using SSR markers. SSR is one of very powerful genetic markers because they are hypervariable, relatively abundant, and provide extensive genome coverage. The primary goal of this project is to survey additional genomic SSR markers for polymorphism between 8 cultivars, and for assessing genetic diversity in sesame. The first draft genome sequence of sesame assembled de novo (274 Mb) of the elite Chinese sesame cultivar Zhongzhi 13 was sequenced. Furthermore, 1500 SSR primer pairs were designed by whole genome survey. Among these, 218 polymorphic SSRs were developed and used to screen 31 sesame accessions. In the current study, we downloaded microsatellite DNAs with a known position on the chromosome in Excel file format (PMDBase database) referring to the first draft sesame genome. We randomly select 99 SSR primer pairs evenly distributed in 16 linkage groups (LGs) based on abundant repeat motifs of di- and tri-nucleotide repeats. Eight sesame cultivars (Milseong, Ansan, Galmi, Kangheuk, Daheuk, Kopoom, Chamhwang and Yangbaek) were evaluated by 99 SSR markers, of which 37 showed polymorphic bands. Among of them, 14 SSR polymorphic markers were reported earlier using 96 sesame accessions collected from 22 countries mainly from Asia and Africa. As a result, we found novel 23 SSR primer pairs amplified abundant, clear and repeatable fragments then they will employ to evaluate the genetic diversity. However, current result is primarily test for 8 Korean cultivars to confirm their suitability. The molecular studies on sesame carrying out for further assessing the level of genetic variation of Korean germplasm collection and worldwide sesame accessions. *Corresponding Author: Tel , asekovasovetgul@korea.kr 159

202 PB-48 Song Lim Kim, Hyenso Ji, Hongseock Lee, Eungyeong Lee, June Oh, Nyunhee Kim, Yoonkook Park, Jungmin Park, Inchan Choi, JeongHo Baek and Taek-Ryoun Kwon, Kyung-Hwan Kim * The initial growth rate is an important physiological trait to improve seedling establishment in direct seeding cultivation of rice. Plant phenomics provides nondestructive analyzing methods using image information of various plant phenotypes. In this study, 162 recombinant inbred lines from a cross between Milyang23 and Gihobyeo (MGRIL) were used for analysis of initial growth using phenomics approaches. The main parameters from RGB images were number of pixels and plant height (PH) in image data. In addition, substantial measurements of shoot length (SL), fresh weight (FW), and dry weight (DW) were performed. Correlation coefficients (R) between image data and substantial measurement were revealed to be high among pixel, fresh weight, and dry weight (T-test<0.01). Several QTLs related with initial growth were found. In the 2 and 4 weeks old seedlings, qpixel-1.1 (interval marker, R1M47-STS01039) and qpixel-1.2 (interval marker, STS01031-STS01039) were nearly located at chromosome 1 with LOD (likelihood of odds) scores of 5.63 and 4.42, respectively. These two pixels related QTLs were similarly located not only with qsl-1.1, qfw-1.1, and qdw-1.1 of 4 week old seedlings, but also with qph-1.1, qsl-1.2, qfw-1.3, and qdw-1.2 of 2 week old seedlings at chromosome 1. All of these QTLs were located at similar location with the semi-dwarf gene, sd-1. These results indicated that number of pixels might reflect plant biomass which is a result of initial growth rate. It is expected that phenomics approach will improve phenotyping accuracy, speeds, and efficiency for QTL analysis of agronomical traits. *Corresponding Author: Tel , biopiakim@korea.kr PB-49 김경훈 *, 김경민, 신동진, 권유리, 현종내, 오명규 국내에서생산되는밀은농가당생산규모가작고, 벼중심의밀재배에따른조기수확등으로품질균일도가떨어져수입밀보다품질이낮게평가되고있다. 이에국내지역별밀품종의품질변이를알아보고자이실험을수행하였다. 각지역별로대표품종에대한품질분석한결과, 전북지역의단백질함량은 8.0% 로작년대비 3.2% 낮아졌고, 전남지역의단백질함량은 9.6% 로작년대비 1.0% 낮아졌으며, 경남지역은 9.3% 로 2.9% 낮아졌다. 추가로수집한경북지역, 충남지역원맥의단백질함량은각각 9.2%, 11.7% 를나타냈다. 모든지역의단백질함량이작년과평년에비해서낮았다. 전분관련요인인아밀로스함량의큰차이는없었고, 단백질의질적특성을나타내는침전가수치는지역간차이보다는품종간차이로, Glu-D1 유전자형 (5+10) 을지닌금강과조경이백중밀보다높은수치를나타냈다. 지역간의단백질함량차이는전남북지역은백중의재배면적비율이높고, 경남북지역은조경의재배면적비율이높은것에기인한것으로생각된다. 특이적으로충남지역의단백질함량이나질적특성면에서도우수한것으로나타났다. 같은품종을재배해도지역별로다른품질의원맥이생산되는점은추후연차별로원맥수집을통해재확인할필요성이있다. 향후 2017 년수확한원맥의품질을추가로조사 분석하여지역별생산되는품종의품질차이를보완 확인할예정이다. * 주저자 : Tel , k2h0331@korea.kr 160

203 PB-50 Young Eun Jang, Min Jeong Hong, Soon-Jae Kwon, Joon-Woo Ahn, Sang Hoon Kim, Yeong Deuk Jo, Hong-Il Choi, Si-Yong Kang, Jin-Baek Kim * Soybean (Glycine max) is one of the major oil crops. Soybean oil is consumed approximately 50 million metric tons per year that placing second highest in world vegetable oil consumption. Soybean seed containing 20 to 25 % lipid and most of the fatty acids are unsaturated fatty acid (18:1, 18:2 and 18:3). Linolenic acid (18:3n-3) is the dietary precursor for the long-chain omega-3 polyunsaturated fatty acids and it is not synthesized by human and other animals. In plant, linolenic acid is desaturased from oleic acid by sequential activity of fatty acid desaturases, FAD2 and FAD3 gene families. We measured seed fatty acid contents of 78 and 154 M 8 mutant progenies from 250 Gy gamma-ray radiated Danbaek (DB) and Daepung (DP) in 2016, respectively. Each 20 mutants of highest linolenic acid were selected from two mutant populations. We investigated agriculture characteristics, such as seed coat color, 100 seed weight, flowering and maturation. According to two year measurement of seed fatty acids, five lines from each mutant population were selected with high linolenic acid content. The average linolenic acid contents of selected lines from DB and DP mutant populations were 9.54% and 9.48%, respectively. For further research, we are searching for nucleotide polymorphism and gene expression changes of FAD2 and 3 gene families during seed development. *Corresponding Author: Tel , jbkim74@kaeri.re.kr PB-51 최인찬, 김송림, 이홍석, 이은경, 김년희, 백정호, 지현소, 권택윤, 김경환 * 최근유럽, 호주와미국등농업선진국에서는식량생산량의증대를위한육종과형질분석을위해식물표현체 (Plant Phenomics) 플랫폼을구축하고표현형연구를하고있다. 이에국립농업과학원은스마트온실에식물운송컨베이어, 이미지센서와챔버그리고워터링시스템을구축하였다. 이시스템은생장양상, 엽색, 엽면적, 물공급량, 비료시비, 스트레스, 형태학등을분석하여식물의특성을파악할수있다. 먼저카메라 Exposure, gain, gamma, 리프트높이및회전속도조합을설정하여선명하고흔들림없는이미지를 2 분 30 초안에획득하여대기시간을단축시켰고펌프의 1 회물공급량과속도, 화분에대한물의총공급량설정, 컨베이어스케줄링, 벼재배에적합한토양을선정하였다. 또한이미지계산값과실측값인픽셀, 엽면적, 크기, 초장, 건물중을이용한피어슨 (Pearson) 상관관계계수 (R) 는 0.96 이상으로시스템의실용성을판단하였다. 이처럼최적화된식물표현체시스템은육안으로판독하기어려운여러가지형질들을고속대량의디지털분석을통해벼육종자원개발및유전체분석기술과연계될수있을것이다. * 교신저자 : Tel , biopiakim@korea.kr 161

204 PB-52 이상복 1*, 현웅조 2, 정종민 3, 이정희 3, 서대하 1, 김병주 2, 성열규 우리나라는 1970 년대통일벼품종의보급됨과더불어벼냉해에대한연구필요성이크게대두되었다 년대의냉해는국내벼재배면적의 64.2% 에결정적인피해를주고약 32% 의수확량감소를가져왔다. 그후 1993 년도냉해는 1980 년도와유사한극심한냉해가왔으나그동안냉해에견디게개량된벼품종의보급으로냉해의피해를크게줄일수있었다 년농촌진흥청작물시험장춘천출장소가설립된이후내냉성검정포장을만들고소양강댐의냉수를이용하여내냉성벼품종육성및유전자원탐색을위한검정사업을지속적으로수행하여왔다. 특히국내벼육종기관에서육성한우량계통들에대한포장및온실에서의내냉성검정을통하여벼품종을육성해온결과내냉성이크게증진되었다. 지난 30 년간국립식량과학원춘천출장소에서벼지역적응시험공시된우량계통에대하여냉수처리로포장검정하고조사한계통들의임실율을보면 80 년대에 29.8%, 90 년대 43.7% 그리고 2000 년대 50.3% 로점차증가하여, 2000 년대평균은 80 년대대비 31%, 90 년대대비약 7% 가증대되었다. 그러나 2010 년이후 2016 년까지연도별우량계통들의냉수처리검정에의한평균임실율은 42% 로감소하고있으며 2014 년이후최근 3 년간은 36.7% 의임실율을보이고있다. * 주저자 : Tel , sabolee@korea.kr PB-53 이홍석, 김송림, 이은경, 오준, 김년희, 백정호, 최인찬, 지현소, 김경환 * 최근이미지센서기반으로식물표현체연구가활발히진행되고있다. 특히생명자원선발효율성증진또는유전자기능대량검정을위한식물표현체분석알고리즘은다양한작물및방법을통해개발되고있다. 본논문에서는국내벼품종에대한생육특성분석알고리즘을연구하였다. 먼저 RGB 카메라를이용하여벼의이미지를획득하고 RGB 이미지를 Lab이미지로변환하여관심영역을추출한다. 추출된관심영역이미지를연산하는방법으로다양한알고리즘을개발하였다. 4개토양, 5개품종에대하여비료투입 5일전과비료투입 15일후의이미지를획득하여 Lab, Lch 색영역에서색상변화를판별하여비료투입여부에대한임계값을설정하였다. 벼의중요생육특성인생체중과건물중의예측을위해서면적, 높이, 폭을영상에서연산하여비교분석하였으며, 상관계수 (R 2 ) 는 0.85 이상이됨을확인할수있었다. 또한 Convex hull, 식물중심점및 Compactness를이용하여새로운생육특성을제시하였다. 본연구는육안으로확인하였던생육특성을개발된알고리즘으로대체가능함을보여주었고다양한알고리즘을통해생육특성에대한수치화, 정형화하여새로운해석방법을제시하고자한다. * 교신저자 : Tel , biopiakim@korea.kr 162

205 PB-54 김년희 1, 김송림 1, 이은경 1, 이홍석 1, 백정호 1, 지현소 1, 최인찬 1, 박부희 2, 김경환 1* 1 2 최근호접란은화훼재배산업에서가장인기있는난초중의하나로주로열대아시아전역에분포하고있다. 부가가치가높은품종을대량생산하기위해조직배양기술을활용하고있으며조직배양결과특이한표현형을보이는변이체가발견되고있다. 국립원예특작과학원에서조직배양을통해육성한 17개호접란의꽃을촬영하고영상처리프로그램 (Image J) 으로꽃의크기, 색깔, 형태학적특징등을이용해표현형을분석하였다. 먼저, 스케일바를이용하여촬영된이미지의단위를결정했고, 이미지의노이즈를제거하였다. 그다음, 꽃의이미지를 R, G, B 채널을이용하여영역추출을하였고, 픽셀값을설정하여배경과꽃을분리하였다. 분리된이미지는히스토그램, 면적, 폭, 높이, 측면의길이, 둥근정도, 퍼짐정도, 균일성등형태학적특징을수치화하고최소-최고표준화를통해꽃의형태, 꽃잎, 꽃받침에대한패턴을분류할수있었다. 본연구는호접란에대한특이표현형분석에있어중요한지표로향후고부가가치난의육종과유전자기능분석에이용될수있을것이다. * 교신저자 : Tel , biopiakim@korea.kr PB-55 이은경 1, 백정호 1, 한희정 1, 김송림 1, 김년희 1, 최인찬 1, 정남희 2, 최만수 2, 문중경 3, 지현소 1, 김경환 1* 콩은식물성단백질및지방산의주요한공급원으로세계적으로중요한식량자원이다. 국립식량과학원에서콩유전체육종의기반구축을위해모집단 4,382점에서재배종 407점, 야생종 393점을선발하여핵심집단 (Core SET-V2) 을작성하였다. 구축된핵심집단은종피색, 엽형, 백립중, 개화일수등의기본형질을조사하였다. 콩의게놈전체연관분석 (GWAS) 을위해핵심집단에서 50계통의종자를 RGB(Red Green Blue) 영상을활용하여디지털표현형분석 (digital phenotyping) 을하였다. RGB영상은위쪽과측면카메라를이용하여촬영하였으며영상분석프로그램 (Image J) 을활용하여콩종자의장폭, 단폭, 두께, 엽색, 제색, 원마도 (Roundness) 등을분석하였다. 특히각계통별영상데이터를명암 (Gray scale), 빨강 (Red), 녹색 (Green), 청 (Blue) 의 4가지히스토그램과로그값으로수치화한결과정상적인패턴이대부분이었으나 4계통은특이한패턴을나타내었다. 이연구를통해실측에의해서분석이어려운콩종자의형태적특성과종피색, 제색등의색상특성을통해게놈전체연관분석 (GWAS) 가능성을탐색하고자하였다. 향후더많은자원을이용하여영상획득, 표현형분석, 유전자기능해석등을통해유전체육종에활용하고자한다. * 교신저자 : Tel , biopiakim@korea.kr 163

206 PB-56 Soo-Hyun Lim, Yeon-Ho Park, Do-Soon Kim * Canopy structure of monocot crop is determined by vertical and horizontal plant growths mainly represented by stem height and stem distribution, respectively, and determine crop biomass yield. Miscanthus species have diverse growth habits and agronomic traits depending on species and thus show high diversity in biomass yield potential. This study was thus conducted to develop methods to assess canopy structure of Miscanthus and to investigate Miscanthus canopy structure in determining biomass yield of Miscanthus. The growth parameters of M. giganteus, selected as a model Miscanthus, associated with canopy structure such as the spatial distribution of stems, stem height, and stem dry weight were assessed at harvest during the four years after planting and its canopy area and volume were estimated. In the first and the second year, canopy area and volume of M. giganteus increased due to slow expansion of stem growth, but from the third year after planting increased dramatically, resulting in dramatic biomass yield. It is suggested that our method and results can be applied to other Miscanthus species for investigating relationship between agronomic traits and canopy structure in determining Miscanthus biomass yield and can be used to find target traits for Miscanthus breeding program. Acknowledgement: This work was carried out with the support of "Next-Generation BioGreen21 Program for Agriculture & Technology Development (Project No. PJ )", Rural Development Administration, Republic of Korea. *Corresponding Author: Tel , dosoonkim@snu.ac.kr PB-57 Jaihyunk Ryu, Soon-Jae Kwon, Yeong Deuk Jo, Jung-Min Kim, Min-Kyu Lee, Dong-Gun Kim, Joon-Woo Ahn, Jin-Baek Kim, Si-Yong Kang * Kenaf seeds have high antioxidant activity due to high amounts of phenolic compounds. This study was carried out to investigate phenolic compounds in three mutant kenaf cultivar (Jangdae, Baekma and Jeokbong) and two original cultivars (Jinju and C14). The phenolic compounds in seeds were determined by HPLC-MS. Three phenolic compounds (N-coumaroyl-3-hydroxytyrosine, ferulic acid and sinapic acid) were identified in early maturing cultivars (Baekma, Jeokbong and C14). Whereas, N-coumaroyl-3-hydroxytyrosine was not detected in mid-late maturing cultivars (Jangdae and Jinju). The ferulic acid content in all cultivars ranged from mg/kg to mg/kg; highest amount of ferulic acid was observed in Baekma. Ferulic acid content in Baekma is approximately 30% higher than it in mother cultivar C14. C14 cultivar showed highest amount of N-coumaroyl-3-hydroxytyrosine. Different amount of sinapic acid was observed between kenaf cultivars; Jinju and Jangdae displayed lowest (25.6 mg/kg) and highest level (924.1 mg/kg), respectively. Findings from the present study indicate that kenaf is a potential source of natural antioxidant and may be utilized for pharmaceutical production. In addition, early maturing cultivars contained N-coumaroyl-3-hydroxytyrosine which can be used as a maker compound to distinguish cultivars. *Corresponding Author: Tel , sykang@kaeri.re.kr 164

207 PB-58 오영진 1, 박태일 1, 김양길 1, 박형호 1, 박종철 1, 강천식 1, 한옥규 2, 윤건식 3, 홍기흥 4, 배정숙 5, 송재기 6, 김경호 1, 정영근 1, 송태화 1, 박종호 1* 청보리는곡류를포함한사료맥류로남부지역을중심으로대부분벼 + 보리작부체계로로전통적인이모작방식으로재배되어왔다. 특히청보리에함유된곡실은고급육생산에유리하며, 배합사료를줄일수있어수입곡물에의존도가높은국내축산농가의사료비절감에크게기여하고있다. 국립식량과학원에서는우수한청보리품종개발에대한축산농가의요구에부응하여 2001 년에도복및내한성이강하며, 초형이양호한 SB00T2064 를모본으로하고, 양질다수성으로총체적성이높은수원 385 호 (IT215831) 를부본으로인공교배하여까락이반매끈망으로부드럽고, 내한성및도복에강하고, 사일리지젖산함량이높아사일리지품질이우수한 ' 미한 ' 을개발하였다. 미한 은직립초형으로초장은 96cm 로 영양 보다크며, 파성은 Ⅳ 정도로가을파종에적합하다. 답리작재배시출수기는 4 월 24 일로영양보다 3 일늦었으며, 황숙기는 5 월 24 일로 영양 보다하루늦었다. 줄기수는m2당 665 개로다얼성이며, 내한성은 영양 보다강하고, 호위축병은나주 (Ⅰ 형 ), 진주 (Ⅳ) 에서저항성, 익산 (Ⅲ 형 ) 에서중도저항으로 영양 과비슷한내재해성품종이다. 조사료건물수량은전작에서 13.8 톤 /ha 으로 영양 에비해 5%, 답리작에서평균 10.6 톤 /ha 로 영양 에비해 1% 감수하였다. 조사료품질은조단백질함량이 10.3%, 총가소화영양분 (TDN) 은 68.2% 로영양에비해다소낮았으나젖산함량이높고초산및낙산함량이낮아사일리지품질이 Ⅰ 등급으로매우양호한품종이다. 미한 의적응지역은 1 월최저평균기온 -8 이상으로북부산간내륙지방을제외한전국에서재배가가능하다. 미한 은추위및도복에도강하고반매끈망으로가축기호성과사일리지품질이좋아금후축산농가들로부터큰호응을얻을것으로기대되며, 금후신품종이용촉진사업을통한종자생산단계를거쳐 2019 년부터농가에보급될예정이다. * 교신저자 : Tel , ark0104@korea.kr PB-59 오영진 1, 박태일 1, 김양길 1, 박형호 1, 박종철 1, 강천식 1, 한옥규 2, 윤건식 3, 홍기흥 4, 배정숙 5, 송재기 6, 김경호 1, 정영근 1, 송태화 1, 박종호 1* 청보리는곡류를포함한사료맥류로남부지역을중심으로대부분벼 + 보리작부체계로로전통적인이모작방식으로재배되어왔다. 특히청보리에함유된곡실은고급육생산에유리하며, 배합사료를줄일수있어수입곡물에의존도가높은국내축산농가의사료비절감에크게기여하고있다. 국립식량과학원에서는우수한청보리품종개발에대한축산농가의요구에부응하여 2005 년에삼차망으로내한성에강하며, 초형이양호한수원 406 호 / 수원 389 호를모본으로하고, 장간으로총체적성이높은 SB961012/ 수원 389 호를부본으로인공교배하여삼차망다수성이면서기호성이우수한우수한 ' 유진 ' 을개발하였다. 유진 은직립초형으로초장은 99cm 로 영양 보다크며, 파성은 Ⅱ 이다. 답리작재배시출수기는 4 월 24 일, 황숙기는 5 월 26 일로 영양 과같다. 줄기수는m2당 696 개로다얼성이며, 내한성은 영양 보다강하고, 호위축병은나주 (Ⅰ 형 ), 진주 (Ⅳ) 에서저항성, 익산 (Ⅲ 형 ) 에서중도저항으로 영양 과비슷한내재해성품종이다. 조사료건물수량은전작에서 17.1 톤 /ha 으로 유연 에비해 19%, 답리작에서평균 11.0 톤 /ha 로 유연 에비해 2% 높다. 조사료품질은조단백질함량이 10.6%, 총가소화영양분 (TDN) 은 69.1% 로유연에비해다소높았으며, 젖산함량이높고초산및낙산함량이낮아사일리지품질이 Ⅰ 등급으로매우양호한품종이다. 유진 의적응지역은 1 월최저평균기온 -8 이상으로북부산간내륙지방을제외한전국에서재배가가능하다. 유진 은삼차망이면서추위및도복에도강하고총체수량이높은다수성으로금후축산농가들로부터큰호응을얻을것으로기대되며, 금후신품종이용촉진사업을통한종자생산단계를거쳐 2019 년부터농가에보급될예정이다. * 교신저자 : Tel , ark0104@korea.kr 165

208 PB-60 Min-Young Kang 1, Hyo-Bong Jeong 1, Ayoung Jung 1, Koeun Han 1, Moo-Chan Kang and Byoung-Cheorl Kang 1* 1 In plants, carotenoid plays important roles in photosynthesis, photo-protection, and phytohormones synthesis such as ABA and strigolactone. In addition, carotenoid provides color to flowers and fruits for attracting insects or animals for pollination and seed dispersal. In Capsicum, ripe pepper fruits display various colors ranging from white to deep red. In the carotenoids synthesis pathway, capsanthin-capsorubin synthase (CCS), phytoene synthase (PSY1), β -Carotene hydroxylase (CrtZ-2) and lycopene β -cyclase (LcyB) genes are known for regulators of fruit color. In this study, total 132 accessions were used for sequence analysis of these genes using single molecule real time (SMRT) sequencing technology. We detected many variants in coding region of the each genes. The nucleotide sequences were aligned to those of red color fruit accessions. Sequencing analysis showed that 5 point mutations and 2 frame shift mutations in the coding region of the PSY1 gene. Among these mutations, one nonsense mutation and 2 frame shift mutations lead to premature stop codon. The CCS gene is consisted of one exon. In the coding region of CCS, 3 missense mutations, 5 nonsense mutations, and 5 frame shift mutations were identified. As for LcyB, we selected 23 accessions which had 4 missense mutations and 1 nonsense mutation. In coding region of the CrtZ-2 gene, 6 missense mutations and 1 frame shift mutation were detected. Specially, sequence analysis of the CrtZ-2 gene revealed a big structural mutation in the non-red color fruits that may result in no expression of this gene. Taken together, we revealed various genetic variations of PSY1, CCS, LcyB, and CrtZ-2 genes which are responsible for non-red color fruit in Capsicum spp. *Corresponding Author: Tel , bk54@snu.ac.kr PB-61 이형운 1*, 이준설 1, 정미남 2, 남상식 1, 양정욱 1, 김재명 3, 안승현 4, 송연상 1, 한선경 5, 황엄지 최근소비자는단맛이강하고찐고구마육질이중간질또는점질로서부드러우면서간편하게먹을수있는적당한크기의고구마를선호한다. 또한고구마의시장가격은조기재배고구마가출하되기시작하는 7 월에가장높고 8 월가격은보통기재배고구마가홍수출하되는 10~11 월보다 30~34% 정도높기때문에 ( 14~ 16 년 3 년평균, 도매상품기준 ) 재배농가는품종선택시식미뿐만아니라조기재배수량성도중요하게고려한다. 고구마신품종 진율미 는농촌진흥청국립식량과학원바이오에너지작물연구소에서식미가우수하고괴근모양과수량성이양호한 신천미 (IT232328)' 를모본으로하고수량성이양호하고찐고구마육질이중간질인 전미 (IT258827) 를부본으로하여 2008 년도에교배하였다 년에실생개체선발시험에서 1,449 개체중괴근의모양과비대가양호한 98 계통을선발하였다 년부터 2011 년까지계통선발시험, 2012 년부터 2013 년까지생산력검정시험을수행하여괴근육색이담황색이고모양, 수량성, 식미가양호한 MI 계통을 목포 90 호 로계통명을부여하여지역적응시험에공시하였다 년부터 2016 년까지 3 년간지역적응시험을수행한결과, 수량성, 품질특성, 병해충저항성등이대비품종인 율미 보다우수하여 2016 년 12 월농촌진흥청농작물직무육성신품종선정심의회에서신품종으로선정되었으며 진율미 (Jinyulmi) 라명명하였다. 진율미 는잎모양이심장형이며, 끝잎색은자색, 엽색과잎자루색, 줄기색은녹색이다. 괴근의껍질색은홍색, 육색은담황색이며찐고구마육질은중간질로부드러운편이고괴근모양은방추형이다. 진율미 는덩굴쪼김병과고구마뿌리혹선충에중도저항성이다. 진율미 는식미점수가 3.6 으로대비품종보다식미가우수하며 ( 대비품종 3.0) 생고구마총유리당함량은 8.8%( 대비품종 4.9%), 감미도는 8.1( 대비품종 4.8) 로대비품종보다각각 80%, 69% 높다. 찐고구마의감미도는 13.4( 대비품종 11.9) 로대비품종보다 13% 높아단맛이더강하다. 진율미 는보통기재배시상품괴근수량이 28.6 MT/ha 이며, 조기재배시상품괴근수량이 24.2 MT/ha 으로대비품종보다각각 19%, 26% 많다. 상품괴근의평균무게는 137g 으로대비품종 (178g) 보다작고주당상품괴근수는 3.6 개로많은편이다. 식미가우수하고조기재배에도적합한 진율미 는농가와소비자모두선호할것으로기대된다 ( 품종출원번호 : 출원 ). * 주저자 : Tel , leehu79@korea.kr 166

209 PB-62 김현철 *, 이현석, 이솔지, 강준원, 임혜민, 이위영, 장경환 2009 년인천서구수도권매립지내에위치한간척지에이태리포플러 3 클론 (Eco28, Dorskamp, Venziano), 미류나무교잡종 3 클론 (Ay48, 97-18, 97-19), 현사시나무 3 클론 (72-30, Bonghwa1, Clivus) 을 1 년수확구, 2 년수확구및 3 년수확구에클론별로각각 1 반복 9 본으로 3 반복총 27 본씩식재밀도 10,000 본 /ha 으로식재한후단벌기목재에너지림조성을위해지상 10 cm 부위에서대절을실시하였다. 수확구별대절에의한맹아지발생수는 1 년수확구에서 11.1 개로가장높았고, 2 년및 3 년수확구에서 7.0, 6.4 개로나타났다. 수확구별연평균바이오매스생산량을비교한결과, 1 년수확구에서평균 2.9 ton/ha/year 로나타났으며, 2 년및 3 년수확구에서각각평균 3.6, 4.3 ton/ha/year 로 3 년수확구의연평균바이오매스생산량이가장우수하였다. 클론별연평균바이오매스생산량은미류나무교잡종들이모든수확구에서다른클론들보다우수하였으며, 특히 및 클론의경우 3 년수확구에서의연평균바이오매스생산량이각각 9.5, 10.8 ton/ha/year 으로나타나가장우수하였다. 간척지환경에서의식엽충 (leaf damage), 천공충 (stem borer damage) 및조기낙엽 (early defoliation) 등의피해도를조사한결과, 식엽충과조기낙엽피해는모든클론이적었으며, 천공충피해가많이나타났다. 바이오매스, 식엽충, 천공충및조기낙엽피해등을지표로사용하여중요도에따라차등을두어가중치를부여한후적응지수를산출하였으며, 그결과미류나무교잡종 및 클론이간척지단벌기목재에너지림에가장적합한포플러클론으로나타났다. * 주저자 : Tel , hckim97@korea.kr PB-63 이현석 1*, 김현철 1, 이솔지 1, 강준원 1, 임혜민 1, 이위영 1 1 테다소나무 (Pinus taeda L.) 는미국남부지방에서광범위하게분포하는것으로알려져있으며우수한생장력과수간통직성으로인하여현지에서는주요경제수종으로이용되고있다. 우리나라에는해방전도입된것으로알려져있으나자료의소실로인하여결과를알수가없으며, 1964 년부터산지시험을통하여리기다소나무와비교하여 3~4 배높은것으로알려져있다. 하지만내한성이약하여우리나라에서생육가능한지역은충청이남으로알려져있다. 최근기후변화로인하여테다소나무의생육가능지확대가능성에대비한우량종자확보및보급의필요성이대두되고있다. 이를위하여가계별로채취된구과및종자특성을구명함으로서, 유용용재수조림을위한종자생산성향상방안을모색하고자본연구를수행하였다 년전남보성에조성된테다소나무시험림에서총 14 가계를선발하였으며, 미국농무성구과분석지침을참고하여종자생산능력, 종자생산효율및고사배주를조사하였다. 구과당총인편수는평균 개로나타났으며, 임성인편수와불임성인편수의평균은각각 63.2 개와 52.6 개로조사되었다. 종자생산능력은평균 으로조사되었으며 73.4 ~ 범위에서나타나가계간최대 2 배가량차이를나타내었다. 종자생산능력은구과당생산가능한종자수의생물학적한계를의미하며구과착과량이증가함에따라종자생산성이저하될수있다. 고사배주수의범위는가계별로 1 차에서 6 52 개, 2 차에서는 개로가계간차이가크게나타났다. 성숙종자수중비립종자와충실종자는각각구과당평균각각 개로조사되었다. 임성인편수는종자생산능력과높은정의상관관계가나타나 (r=0.80, p<0.01), 임성인편수가증가할수록종자생산능력이높아지는것을확인할수있었다. * 주저자 : Tel , elvangood@korea.kr 167

210 PB-64 박승민 1*, 김기용 2, 김지혜 1, 김영진 1, 김원호 본연구는최근확산되고있는이탈리안라이그라스의무인헬기파종과기존의산파및조파파종방법의생육특성을비교하기위해수행하였다. 시험은국립축산과학원축산자원개발부논시험포장 ( 천안소재 ) 에서 2017 년 3 월 9 일부터 5 월 30 일까지수행하였으며, 공시재료는이탈리안라이그라스 (IRG) 코윈어리품종을이용하였다. 파종방법은무인헬기, 조파및산파를하였으며, 파종량은무인헬기및미스트기산파시 60kg/ha, 조파시 40kg/ha 으로하였다. 시비는전량기비로하였으며, 시비량은 N-P 2 O 5 -K 2 O = kg/ha 을살포하였으며, 파종후답압하였다. IRG 수량성을비교한결과, 무인헬기시험구가조파및산파에비해수량성이낮았으며, 조파시험구에서수량성이가장높게나타났다. 초장은무인헬기파종이 90cm 로가장컸으며산파에서가장작았다. 생초수량은조파가 27,000kg/ha, 산파가 25,700kg/ha 및무인헬기파종에서 22,700kg/ha 로나타났다. 건물수량에서는조파가 5,967kg/ha 로가장많았으며미스트기산파가 5,680kg/ha, 무인헬기가 5,017kg/ha 였으며, 건물률은시험구모두 22.1% 로나타났다. 이탈리안라이그라스파종방법에서조파가가장우수한것으로나타났으며, 무인헬기파종시는산파에비해 88% 의수량성을보였다. * 주저자 : Tel , seung7min@gmail.com PB-65 이솔지 1,2*, 김현철 1, 이현석 1, 임혜민 1, 강준원 1, 이위영 1, 김판기 1,2 1 2 본연구는우리나라의기후풍토에적합한용재수를육성하기위해서종간교잡육종된리기테다소나무의생장특성과재적생장에대한육종효과를검증 고찰하였다. 공시재료는서울대학교광양연습림에조림된 47 년생리기테다소나무와이것의육종모수종 ( 育種母樹種 ) 인테다소나무 (49 년생 ) 와리기다소나무 (43 년생 ) 5 개체씩이다. 생장량분석은공시재료를지상 0.2m 에서벌도하여 1-2m 간격으로원판을채취한다음, 수간석해를 huber 식구분구적법에따라서실시하였다. 그결과, 1 영급생장기간 (1~10 년 ) 중의연평균수고생장량은테다소나무가 0.56m 로리기다소나무 (0.37m), 리기테다소나무 (0.44m) 보다높은값을나타냈다. 연평균흉고단면적생장량은테다소나무가 3.83cm 2 로리기다소나무 (2.33cm 2 ), 리기테다소나무 (2.74cm 2 ) 보다높은값을나타냈다. 그리고연평균재적생장량은테다소나무가 m 2 로리기다소나무 (0.0009m 2 ), 리기테다소나무 (0.0018m 2 ) 보다높은값을나타내, 테다소나무가가장좋은생장을나타내고있음을알수있다. 2 영급생장기간 (11~20 년 ) 중에는 1 영급과마찬가지로영급생장기간 (11~20 년 ) 중의평균수고생장량은테다소나무가 0.80m 로리기다소나무 (0.46m), 리기테다소나무 (0.59m) 보다높은값을나타냈다. 평균흉고단면적생장량은테다소나무가 10.5cm 2 로리기다소나무 (6.90cm 2 ), 리기테다소나무 (8.34cm 2 ) 보다높은값을나타냈다. 그리고평균재적생장량은테다소나무가 m 2 로리기다소나무 (0.0037m 2 ), 리기테다소나무 (0.0059m 2 ) 보다높은값을나타내테다소나무가좋은생장을나타내었다. 3 영급생장기간 (21~30 년 ) 도마찬가지로평균수고생장량은테다소나무가 0.70m 로리기다소나무 (0.47m), 리기테다소나무 (0.58m) 보다높은값을나타냈다. 평균흉고단면적생장량은테다소나무가 10.88cm 2 로리기다소나무 (8.35cm 2 ), 리기테다소나무 (9.44cm 2 ) 보다높은값을나타냈다. 평균재적생장량은테다소나무가 m 2 로리기다소나무 (0.0062m 2 ), 리기테다소나무 (0.0092m 2 ) 보다높은값을나타내테다소나무가좋은생장을나타내었다. 4 영급생장기간 (31~40 년 ) 에들어서면서평균수고생장량은테다소나무가 0.61m 로리기다소나무 (0.41m), 리기테다소나무 (0.53m) 보다높은값을나타냈다. 평균흉고단면적생장량은테다소나무가 10.88cm 2 로리기다소나무 (9.13cm 2 ), 리기테다소나무 (10.44cm 2 ) 보다높은값을나타냈다. 그리고평균재적생장량은테다소나무가 m 2 로리기다소나무 (0.0099m 2 ), 리기테다소나무 (0.0121m 2 ) 보다높은값을나타내테다소나무가좋은생장을나타낸다. 그리고전생장기간동안의평균재적생장에서도테다소나무가 m 2 로리기다소나무 (0.0045m 2 ) 와리기테다소나무 (0.0068m 2 ) 에비하여좋은재적생장을나타낸다. 이러한결과로본연구의시험지역에서는재적생장에대한리기테다소나무의육종효과는기대할수없으나, 테다소나무가리기다소나무나리기테다소나무보다용재수조림에적합한수종임을알수있다. * 주저자 : Tel , solji730@korea.kr 168

211 PB-66 MyoungHee Lee *, JiMyoung Choi, EunYoung Oh, SungUp Kim, JungIn Kim, SukBok Pae, KiWon Oh, UnSang Yeo, DoYeon Kwak Perilla is one of oilseed crops in Korea and its seed is composed of lipid 35 ~ 45%, protein 20 ~ 25%. The proportion of omega-3 among fatty acids(α -linolenic acid) in Perilla oil is over 60%, which is the highest percentage among vegetable oils. A perilla variety Deulhyang (Perilla frutescens (L.) Britton) for edible seed was developed at the Department of Southern Area Crop Science, NICS, in Milyang in It was originated from the cross between a pedigree of K with white seed and a few branch, and YCPL647 with early maturity in Deulhyang has white and soft seed, so its characters showed a great advantage to make a perilla powder in terms of color favor and yield as compared with brown seed. Deulhyang has 100cm of main stem length and 12 branch numbers per plant, which is 4 numbers fewer than that of check variety Saeyeopsil. Maturing date of Deulhyang was September 30, which is 5 days faster than that of Saeyeopsil. This variety has high oil content (45%) and linolenic acid(63%) in the fatty acid composition. The yield potential of Deulhyang was about 1.24 ton/1ha in the regional yield trial. *Corresponding Author: Tel , emhee@korea.kr PB-67 Da-Young Baek 1*, Kyung Jun Lee 1, Myoung-Jae Shin 1, Gi-An Lee 1, Yang-Hee Cho 1, Kyung-Ho Ma 1, Yoon-Sup So 2, Jung-Ro Lee Black soybean (Glycine max L. Merr.) has been used as an excellent dietary source for the disease prevention and health promotion in Korea for hundreds over years. This study was carried out to evaluate the antioxidant activity and to determine the flavonoid contents in the 231 Korean black soybean landraces(kbsl), which conserved at RDA Gene bank. Antioxidant activities in the 231 KBSL were measured by using DPPH, TPC, TFC, ABTS and FRAP assay. The antioxidant activity assay of 231 KBSL showed the averages as follows; DPPH, 61.4 ± 33.4 (IC50); TPC, 7.0 ± 2.8 mg gallic acid equivalent/g; TFC, 0.53 ± 0.15 mg quercetin equivalent/g; ABTS 4.9 ± 1.6 mg ascobic acid equivalent(asc)/g; and FRAP 1.10 ± 0.62 mg ASC/g. Based on the results of antioxidant activities, 31 representative KBSL were selected by using POWER CORE program. To compare the flavonoid content related to antioxidant activity, we measured the contents of myricetin, quercetin, kaempferol, and naringenin among the 31 representative collections. Myricetin contents of 231 KBSL were ranged from 0.06 to 0.23 mg/100g and IT showed the highest myricetin content among them. Quercetin contents were ranged from 0.05 to 1.19 mg/100g and IT showed the highest. Kaempferol and Naringenin were ranged from 0.15 to 1.66 mg/100g and 0.06 to 0.63 mg/100g, respectively and IT showed the highest kaempferol and naringenin content. This study will be able to provide useful data for selecting seeds with high antioxidant activity and flavonoid content. *Presenting Author: dayung96@naver.com 169

212 PB-68 Kyung Jun Lee 1*, Myoung-Jae Shin 1, Jung-Ro Lee 1, Yang-Hee Cho 1, Kyung-Ho Ma 1, Gi-An Lee 1 1 Adzuki bean (Vigna angularis L.) is cultivated around the world, mainly in Asiatic countries, as diverse food sources. In Korea, the leaves of adzuki bean has been used as medicine and side dishes. Sikryochanyo, the Korean traditional medical book, described the leaves of adzuki beans that are helpful in treating and preventing diabetes. In this study, we investigated the antioxidant activity in the leaf extracts of 222 Korean adzuki bean landraces (KABL). Antioxidant activities of adzuki bean leaves extracts were analyzed by using DPPH, ABTS, ferric reducing antioxidant power (FRAP), reducing power (RP) total phenolic content (TPC), and SOD assays. Among the six antioxidant activities assay, DPPH showed wide variations, ranging from 12.6 to (IC50, CV 81.3%). ABTS, FRAP, RP, TPC and SOD in KABL ranged from 1.48 to 2.50 mg ASC/g, 0.39 to 6.03 mg ASC/g, 2.9 to 147 mg GAE/g, 0.37 to 14.7 mg ASC/g, and 7.8 to 38.2 (IC50), respectively. According to the relative antioxidant capacity index (RACI), IT (1.6) had the highest antioxidant activity, while IT (-2.3) was the lowest. In clustering analysis, 222 KABL were classified into five groups. These results will expand the database for antioxidant activity and provide information on KABL which are valuable for development of functional foods and feed-additives resources. *Presenting Author: Tel , lkj5214@korea.kr PB-69 Xiao-Han Wang 1*, Kyung Jun Lee 1, Myoung-Jae Shin 1, Kyung-Ho Ma 1, Gi-An Lee 1, Yang-Hee Cho 1, Jung-Ro Lee 1 1 Beans are important dietary components with versatile health benefits. We analysed the extracts of 211 Korean common bean (Phaseolus vulgaris) landraces in order to determine their antioxidant activity. The antioxidant activities were measured by using total polyphenolic content (TPC), ABTS and FRAP assay. The results revealed that IT had the highest TPC (3.46 mg gallic acid equivalent (GAE)/g) and FRAP (2.76 mg ascobic acid equivalent (ASC)/g). In ABTS assay, IT showed the highest antioxidant activity (1.49 mg ASC/g). TPC of 211 Korean common bean landraces showed a range of 0.60 to 3.46 mg GAE/g. FRAP and ABTS showed a range of 0.32 to 2.76 and 0.28 to 1.49 mg ASC.g, respectively. According to relative antioxidant capacity index (RACI), IT (2.05) had the highest antioxidant activity, while IT (-2.2) was the lowest. In clustering analysis, 222 common landraces were classified into four groups. This study will be able to provide useful data for selecting common bean landraces with high antioxidant activity. *Presenting Author: Tel , wang@knu.ac.kr 170

213 PB-70 Ga-Hee Kim 1*, Kyung Jun Lee 1, Myoung-Jae Shin 1, Gi-An Lee 1, Yang-Hee Cho 1, Kyung-Ho Ma 1, Yoon-Sup So 2, Jung-Ro Lee The aim of this study was to compare the tocopherols composition from the germplasms of Korean black soybean (Glycine max L.) landraces. Tocopherols are well known for their beneficial effects on human health such as antioxidant and anti-inflammatory activities. In this study, tocopherol contents in the seeds of 231 Korean black soybean landraces were analyzed using HPLC. Total tocopherol contents of 231 Korean black soybean landraces ranged from 5.4 to ug/g with average of 80.7 ± 44.4 ug/g. The contents of α -tocopherol, β +γ -tocopherol δ -tocopherol ranged from 2.3 to 327.3, 7.0 to 336.0, and 6.1 to ug/g, respectively. Among 231 Korean black soybean landraces, IT showed the highest total tocopherol contents. The highest contents of α -tocopherol, β +γ -tocopherol δ -tocopherol were found in IT177459, IT177584, and IT respectively. Among them, IT had the lowest contents of all of tocopherols. These results describe the variations of tocopherol composition in the Korean black soybean landraces. The data obtained would be useful to both consumers and producers for manufacturing of traditional black soybean, which are commonly used in many countries. *Presenting Author: Tel , dayung96@naver.com PB-71 Sea-Hee Han *, Jung-Ro Lee, Gi-An Lee, Kyung-Ho Ma The National Agrobiodiversity Center (NAS, RDA, Republic of Korea) has been committed to collect new valuable genetic resources continually. In this study, we regenerated conserved Capsicum spp. germplasm which could not be available due to seed quantity and quality, and we also investigated their morphological characters for sustainable utilization. A total of 192 Capsicum spp. accessions were regenerated and 15 morphological traits were surveyed according to the characterization guideline of RDA Genebank. Among the surveyed traits, flowering time ranged from June 7 to July 22 and 85% of tested accessions were mainly flowering in June. The maturity time ranged from June 3 to October 18 and main flowering time was October (45.6%) and September (37.8%). Main flower colors (FC) were white (46.6%) and pale green (43.0%). The fruit shape (FS) was various; flat round (7.8%), round (7.3%), heart shape (6.2%), quadrangle (10.9%), rectangle (6.7%), trapezoid (5.7%), triangular (13.0%), elongated (36.3%), Horn (5.7%). Principal component analysis (PCA) showed four principle components included PC-Ⅰ (32.6%), PC-Ⅱ (12.2%), PC-Ⅲ (8.3%), and PC-Ⅳ (7.4%). Cumulatively four components explained 60.5% of the total variation in the data. PC-1 showed positive effect for some specific traits such as FC, maturity, fruit weght. PC-2 was more related to FC and FS, and PC-3 and PC-4 exhibited positive effect for plant type and density of stem hair. Our results provides insight into the characteristics of Capsicum spp., and the utilization basis of Capsicum spp. might be elevated for bio-industry. *Presenting Author: Tel , @naver.com 171

214 PB-72 Hyo-Yong Kim 1, Yun-Shil Gho 1, Ki-Hong Jung * 1 Little and tiny organ, ligule is able to have an effect on whole plant. The ligule is a thin and tongue-like white membrane in some plants including Rice. In rice, the ligule works many things as a column, umbrella and humidifier. However, we actually don t know the ligule specific gene and sequence. To effectively address this limitation, selection of useful candidate genes and identification of major regulatory factors through global approaches are necessary. So This paper used meta-expression analysis data and network analysis data from NCBI Gene Expression Omnibus about collar preferred gene to find out ligule specific gene. And identified 654 rice genes commonly differentially expressed under collar specific conditions. Gene ontology enrichment analysis show the quality and correlation about selected genes for ligule specific genes. Additionally, Regulation, Metabolism, Transcription and biotic stress overview were predicted with MapMan analysis. Using these methods, we will analyze the suitability of candidate genes. uncovering veiled information of the ligule specific genes should contribute to increase major crops production and aid additional experiment. *Corresponding Author: Tel , khjung2010@khu.ac.kr PB-73 Jeeyeon Ko 1*, Seokbo Song 1, Myeongen Choe 1, Kwansik Woo 2, Taewook Jeong 2, Doyeon Kwak 1, Kiyoung Kim 3, Jongcheol Ko 4, Inseok Oh Barnyard millet Echinochloa esculenta (A. Braun) is one of the small grain crops of millets, having balanced nutrients and abundant minerals. Recently there has been a growing interest in barnyard millet because it has characteristics that can be grow both in dry upland and waterlogged paddy filed. Borajik, newly developed barnyard millet variety, was developed at the Department of Southern Area Crop Science, NICS, in Miryang in It was originated from K in 2009 by pure line selection. The cultivation period of Borajik were 95days at seeded in normal season(15th May) and 85days at seeded in double cropping season(15th June) which were 7 9 days later than that of check variety Sodamjik. The column length were 120cm at seeded in normal season and 137cm at seeded in double cropping season and showed resistance to lodging. The yield potential of Borajik were about 3.16 ton/ha at seeded in normal season(15th May) and 3.80 ton/ha at seeded in double cropping season(15th June) in replicated yield trials(ryt), which were 33 74% increased than that of check variety Sodamjik. The endosperm characteristic of it showed non-waxy having 27% of amylose contents. *Corresponding Author: Tel , kjeeyeon@korea.kr 172

215 PB-74 Eun-Young Oh *, Myung-Hee Lee, Jung-In Kim, Sung-Up Kim, Suk-Bok Bae, Un-Sang Yeo, Sovetgul Asekova, Ki-Won Oh, Do-Yeon Kwak This study was carried out to investigate the quality of perilla (Perilla frutescens var.frutescens) seeds using non-destructive and time-saving Near-Infrared Spectroscopy method. Samples were tested on Unity Scientific Spectro2500-XL, which has the wavelength ranging from 680 to 2500nm by an interval of one. Crude fat, protein, rosmarinic acid content and fatty acid composition were analyzed by soxhlet extraction, Dumas combustion, liquid chromatography and gas chromatography, respectively. For calibration equation. 215 perilla lines including varieties and germplasms were tested for both NIR and component analysis. Among the perilla seeds, crude fat contents ranged from 27.4 to 51.2% at the average of 37.3% (R 2 =0.985), protein contents varied from 14.5 to 30.8%, which has average of 19.5% (R 2 =0.936). Rosmarinic acid content has 823 to 2815μg /g of variation and the average value of 1490μg /g (R 2 =0.844). Coefficient of correlation of palmitic acid (C 16:0), stearic acid (C 18:0 ), oleic acid (C 18:1 ), and linoleic acid (C 18:2 ) composition were 0.896, 0.784, 0.914, 0.869, respectively. Linolenic acid (C 18:3 ) varied from 51.1 to 68.6%, which has average composition of 56.4% (R 2 =0.937). Each tested component showed the strong positive linear correlation between NIR value and wet analysis. This NIR equation could be a useful tool for selecting line and gathering quality information in perilla breeding. *Corresponding Author: Tel , lavondy10@korea.kr PB-75 Suk-Bok Pae 1*, Myung-Hee Lee 1, Sung-Up Kim 1, Eun-Young Oh 1, Un-Sang Yeo 1, Ki-Won Oh 1, Deok-Young Song 2, In-Seok Oh 1, Yong-Chul Kim Korean usually like to consume large grain of peanut for the roasted or boiled. One of peanut breeding programs is also focused on developing large grain cultivar. A new peanut variety "Tamsil"(Arachis hypogaea ssp. hypogaea L.) with large grain and short stem was developed in the Department of Southern Area Crop Science, NICS, in Milyang This variety was developed from the crossing line between variety "Charmpyeong(Milyang26)" with short stem Virginia plant type and line "Milyang20" with semi-erect Virginia plant type. "Tamsil" which is short stem and semi-erect Virginia plant type has 31cm of main stem length, 42cm of branch length and 22 branch number per plant. Each pod has two grains with ellipse shape of brown testa and its yield components are composed of 44 mature pods of per plant, 111g of 100-seed weight, 78% of pod shelling ratio in the regional yield trials(ryt). Its seed quality show 28.8% of crude protein and 48.5% of crude oil and 53.2% of oleate in fatty acid composition. This also show resistant to early leaf spot, and more resistant to web blotch, stem rot, and lodging, compared with check variety Daekwang. Owing to these superior growth characteristics of lodging resistance and source ability in late maturing stage, the average grain yield of "Tamsil" was more productive than reference variety by 19% with 4.97 MT/ha for grain production in 3 year regional yield trials. *Corresponding Author: Tel , paesb@korea.kr 173

216 PB-76 Yeong Deuk Jo 1, Han Sol Kang 1, Sang Hoon Kim 1, Jaihyunk Ryu 1, Jin-Baek Kim 1, Si-Yong Kang 1* 1 Mutation breeding based on irradiation has been widely used for development of useful genetic resources for plant breeding. We have been developing mutation populations using diverse radiation sources, irradiation methods, and plant materials in pepper. First of all, M 2 M 3 populations that consist a total of 4,490 individuals were developed in 'Yuwolcho', a Korean landrace (C.annuum L.) that has early-flowering characteristics, by irradiation of gamma-rays and carbon ion beam, respectively, as standard populations for forward and reverse genetics studies. In the second place, we are developing M 1 population that consist of 1,666 individuals of 'Habanero', a C.chinense cultivar which is famous for its extreme pungency, to provide useful materials for practical breeding of cultivars for export and production of functional materials. Finally, populations were developed from crosses using irradiated pollens or pistils aiming the broader mutation spectrum or higher efficiency. In combination with optimized TILLING system based on capillary electrophoresis, these population will be used for breeding and functional genomics studies in pepper. *Corresponding Author: Tel , sykang@kaeri.re.kr PB-77 현웅조 1*, 이정희 5, 원용재 1, 정국현 1, 안억근 1, 이상복 2, 김명기 1, 하운구 1, 양창인 3, 성낙식 1, 조영찬 4, 강경호 4, 서대하 2, 김병주 우리나라식량자급률제고를위해중부지역은다양한식량작물이재배되고있으며이러한작물들을효율적으로재배하기위해다양한작부체계가필요하다. 작부체계의성공을위해서는작물재배기간이중첩되는것을피해야하고이를위해벼를일반재배보다빨리재배하는조기재배또는늦게재배하는만기재배에적응할수있는벼품종개발이필수적이다. 중부지역에적응하는밥맛이우수한조생품종개발을목적으로진광은단교배된운광 / 고시히까리 F1 과호품을각각모부본으로하여 2007 년에교배되었다. 세대단축을위해 F1 세대의약을배양하여 AC3 세대에서초형과쌀품질이우수하고도열병및흰잎마름병저항성을보인 SR HB 을선발하여수원 575 호의계통명을부여하였다. 2 년간의생산력검정시험과 3 년간의지역적응시험결과그우수성이인정되어 2016 년직무육성신품종심의회에서최고품질벼로선정되었다. 진광 은조생종으로서밥맛과재배안정성이우수하여생산자부터소비자까지만족할수있고다양한작부체계에적응할수있는품종으로역할이기대된다. 사사 : 본연구는농촌진흥청연구사업 ( 세부과제명 : 조 중생고품질벼품종개발, 세부과제번호 : PJ ) 의지원에의해이루어진것임. *Corresponding Author: Tel , greathyunwj@gmail.com 174

217 PB-78 손지영, 박여옥, 김은경, 이영한, 최성태, 김성철, 안광환, 박두상 단감의주요재배품종인 부유 는 11 월에수확이가능한만생종으로수확기서리피해, 노동력집중등많은문제점을가지고있다. 따라서수확이빠르고고품질의특성을지니며떫은맛의우려가없는완전단감품종의육성이요구된다. 완전단감은열성형질로완전단감과의교배조합에의해출현될확률이월등히높다. 고품질의완전단감을육성하기위해서는요구형질을지닌고품질의완전단감품종의수가많아야하지만완전단감품종의수는극히제한적이기때문에육성에어려움이많다. 따라서단감연구소에서는완전단감이면서수확기가빠르고고품질의특성을지니는품종을육성하고자시험을수행하였다. 단연 는 2009 년완전단감 양풍 을모본으로, 태추 를부본으로교배하여얻은완전단감계통이다. 숙기가 10 월 17 일로 부유 에비해빠르고당도는 15 Brix 로 부유 와비슷하다. 과중이 370g 으로대과종에속한다. 과실모양은장형이다. 육질이다소치밀하고감꼭지부위의과육심부위가넓은특성을보이지만, 단감말랭이로가공하였을때우수한식감과품질을나타냈다. 따라서, 수확기가빠른고품질완전단감육종의중간모본용으로사용가치가높고, 단감가공용으로의효용가능성도있어이계통을선발하였으며, 추후세부특성조사수행후품종보호출원예정이다. Author PB-79 김경훈 *, 윤성란, 신동진, 권유리, 김경민, 한상익, 현종내, 오명규 유색밀은종피에자색또는흑색이착색되어색을띄는밀이다. 이러한유색밀자원의색소함량분석을위해서추출분석방법을설정하고자한다. 식품원료에서색소가함유되어있는페놀산추출분석시유기용매로추출하는방법이주로이용되고있으나추출하고남은잔사에도결합형형태의페놀산등의유용한물질이존재하고있다. 그러므로이실험에서는유색밀의색소성분추출시기존의추출방법인에탄올, 메탄올등의용매별분석하는방법과가수분해를통한결합형의페놀산까지분석하는방법을비교해보았다. 실험재료로유색밀인밀양 42 호, 일반밀 ( 금강 ) 등을통밀가루로분쇄하여 50% 아세톤, 80% 메탄올, 80% 에탄올용매로추출하는방법과알칼리가수분해방법을이용하여결합형탄닌, 총페놀성화합물, 항산화능등을분석하였다. 총페놀성화합물은가수분해, 50% 아세톤, 80% 메탄올, 80% 에탄올용매순으로함량이많았다. 유색밀의결합형탄닌, 총페놀성화합물의함량은각각 ±83.27( μg catechin Eq./g), ±18.37( μg GAE/g) 로일반밀인금강밀에비해서모두높았다. 항산화능분석으로 Trolox equivalent antiocidant capacity(teac) 은 ±28.64( μg trolox Eq./g) 로서금강의 ±4.77 보다높았다. 그러므로가수분해방법은결합형페놀성함량까지알수있지만항산화능을측정할수없으므로, 추출용매중에서는 50% 아세톤을이용한추출방법이가장적합한것으로생각된다. * 주저자 : Tel , k2h0331@korea.kr 175

218 PB-80 Kyung Eun Lee 1, Jegeun Cho 1, Jong Bae Kim 2, Sang Gu Kang 1* 1 2 Rice Purple Pericarp (Prp) trait is required primarily to make black rice. Prp trait was known as a recessive epistasis following the Mendelian 9:3:4 ratios of purple, brown and white, respectively. However, self-pollination of the F 1 hybrid Pbpb Pppp genotype generated 4 different patterns of progenies with 3 dark purple : 6 medium purple : 3 brown : 4 white, resulted in differ to the typical recessive epistasis. The biochemical analysis of the progenies divided evidently into two groups; one contained cyanidin 3-O-glucoside (C3G) in the dark and medium purple seeds, but the other showing absence of C3G in brown and white seeds, resulting in the ratio of 9 C3G: 7 absences C3G, which fitted to the extended Mendelian 9:7 ratios of complementary gene interaction. Although back rice has been defined in either recessive epistasis or complementation depending on researchers, here we confirmed the Prp trait is defined as a complementary gene interaction of the Pb and Pp genes. Moreover, by assist with Pb allele genotyping, breeding convenience was achieved to select the black rice lines fixed with the two dominant alleles of Pb and Pp genes among complex allelic constitutions of the two genes in the hybrid progenies. *Corresponding: kangsg@yu.ac.kr PB-81 Paulina Calderón Flores, Yong Weon Seo * The grain color of wheat has been related to the brightness of flour and to tolerance to preharvest sprouting. Grain color is controlled by dominant R-1 genes located on the long arm of hexaploid wheat chromosomes 3A, 3B, and 3D (R-A1, R-B1, and R-D1, respectively). The R-1 genes affect the sensitivity of embryos to abscisic acid (ABA) and the development of grain dormancy. Tamyb10-A1, Tamyb10-B1, and Tamyb10-D1 genes, located on chromosomes 3A, 3B, and 3D, respectively. The red pigment of the grain coat is composed of catequin and proanthocyanidin (PA). These genes encode R2R3-type MYB domain proteins, similar to TT2 of Arabidopsis, which controls PA synthesis in testa. A single dominant allele is sufficient to result with red color, but grain redness increases in a gene-dosage-dependent manner. We choose publicly available markers which are known to related to the seed coat and the dormancy level based on the R gene of Yellow-Red wheat seeds to see if it is also related to the seed dormancy and testa color in our materials. We concluded that the testa color might not be a factor to determine dormancy in wheat or at least is not related to the deep purple testa color from our samples. Acknowledgement: This study was supported by the Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ ), Rural Development Administration, Republic of Korea. Paulina Calderón Flores acknowledges CONACyT for financial support to follow doctoral studies. *Corresponding Author: Tel , seoag@korea.ac.kr 176

219 PB-82 김양길 1*, 이미자 1, 박종철 1, 김경호 1, 강천식 1, 박종호 1, 한옥규 1, 최진경 2, 배정숙 3, 송재기 4, 박태일 1, 정영근 1, 김보경 최근다양한건강식품이개발되어소비자들의관심이높아짐에따라채소는물론곡류에있어서도혼반용곡류의소비가증가하고있다. 따라서이에적합한품종을육성하기위해 2004 년에쓰러짐에강하고다수성등특성을가진 수영보리 를모본으로, 출수가빠른 극조숙 55 계통을부본으로인공교배하여. 도복에강하면서다수성인혼반및보릿가루용메성쌀보리 호강 을개발하였다. 호강 은 6 조이며파성이 Ⅱ 인병성쌀보리로이삭의형태는중소수형이고, 까락이길며탈부성이좋다. 출수기는새쌀보리에비해익산 ( 전주 ), 나주, 진주, 대구에서 4 월 18 일로 1 일빨랐다. 간장은 69 cm로새쌀보리보다 5 cm정도짧은중간형으로내도복성이며, 수장 (6.2 cm ) 은길고 1 수립수 (65 개 ) 가많다. 천립중 (34.6g) 은새쌀보리보다 4.1g 무거웠다. 병해저항성중보리호위축병은저항성이며, 내한성은새쌀보리와비슷하였다. 호강 은단백질 (10.1%), 아밀로스 (21.7%) 함량이비슷하나베타글루칸 (5.5%), 백도 (37.6) 높고, 흡수율, 퍼짐성이좋았다. 조곡수량성은 4.56 톤 /ha 으로 7% 증수하였다. 호강 은 1 월평균기온이 6 이상인지역에보급될것으로기대된다. * 주저자 : Tel , kim5yk@korea.kr PB-83 김양길 1*, 박종철 1, 김경호 1, 강천식 1, 박종호 1, 한옥규 1, 윤건식 2, 배정숙 3, 조수현 4, 박태일 1, 정영근 1, 김보경 최근곡물을이용한차음료시장확대에따른다양한기능성음료가개발되어소비자들의관심이높아지고있다. 따라서이에적합한보리차음료용품종을육성하기위해 2005 년에보리호위축병에강하면서재해에저항성인특성을가진 새강보리 를모본으로, 고품질의 두루보리 품종을부본으로인공교배하여. 써러짐에강하고다수성인보리차용겉보리 호향 을개발하였다. 호향 은 6 조이며파성이 Ⅱ 인병성겉보리로이삭의형태는밀수형이고, 까락이길며탈망성이좋다. 출수기는올보리에비해전작 ( 수원, 춘천, 청원 ) 에서 4 월 23 일로 1 일, 답리작 ( 대구, 익산, 전주 ) 에서 4 월 18 일로 1 일빨랐다. 간장은 78 cm로올보리와비슷한장간형으로내도복성이며, 수장 (4.7 cm ) 은길고 1 수립수 (55 개 ) 가많으며, 천립중 (31.9g) 은올보리보다 3.6g 가벼웠다. 병해저항성중보리호위축병은올보리보다저항성이며, 추위저항성정도는올보리와비슷하였다. 호향 은단백질 (10.9%) 과베타글루칸 (5.0%) 함량은올보리와비슷하나폴리페놀함량 (0.19%) 이높다. 보리차특성으로서볶음처리에의한동일한조건에서의보리차색도 (L) 가진하고, 적색 (a) 및황색 (b) 높았다. 조곡수량성은전작에서 6.68 톤 /ha 으로 6% 증수, 답리작 6.03 톤 /ha 으로 9% 증수하였다. 호향 은 1 월평균기온이 8 이상인지역에보급될것으로기대된다. * 주저자 : Tel , kim5yk@korea.kr 177

220 PB-84 Mia Park 1, Gregory B. Tilton 2, Erin Bell Maize (Zea mays L.) is known as a highly genetically diverse species, which is reflected in the considerable natural variability in composition. The paper reviewed here provides an in-depth compositional analysis of a set of hybrids based on Nested Association Mapping (NAM) founder lines and landraces that were selected for their genetic diversity, and documents the variability in the levels of a large set of grain components. 25 inbred lines selected to represent genetic diversity in maize (NAM inbreds) and 24 inbred lines derived from a diverse collection of landraces were hybridized with B73, an inbred line that has a high-quality reference genome. These hybrids were planted in 2012 in a replicated trial at a single location in the United States. Components from the harvested grain were analyzed for proximates, fiber, minerals, amino acids, fatty acids, tocopherols, β -carotene, phytic acid, and raffinose. Results reported in the publication demonstrated that while composition segregated by group (ex. tropical vs. temperate varieties) extensive variation existed across all grain components assessed for both the NAM and landrace hybrids, reflecting the underlying genetic diversity of these lines. The results from these hybrids are important because they provide a first survey of grain composition in hybrids from two important genetic resources, the NAM founder lines and landraces. Title of paper: Compositional Assessments of Key Maize Populations: B73 Hybrids of the Nested Association Mapping Founder Lines and Diverse Landrace Inbred Lines. Tyamagondlu V. Venkatesh, George G. Harrigan, Tim Perez, and Sherry Flint-Garcia (J. Agric. Food Chem. 2015, 63, ) Corresponding presenter: Tel , mia.park@monsanto.com PB-85 Ki-Won Lee *, Md. Atikur Rahman, Sang-Hoon Lee, Ki-Yong Kim, Hee Chung Ji, Tae Young Hwang, Gi Jun Choi Tall fescue (Festuca arundinacea Schreb.) is a Gramineae perennial grass species and commonly used as a forage crop and developed to be used for soil retention or as lawn. A new tall fescue variety named Greenmaster2ho and Greenmaster3ho was developed by the National Institute of Animal Science, Rural Development Administration, in Cheonan, Korea from 2010 to For the synthetic seed production of this new variety, 5 superior clones were selected and polycrossed. The agronomic growth characteristics and forage production capability of the seeds were studied at Cheonan from 2010 onward, and regional trials were conducted in Cheonan, Hoengseong, Jeju, and Jinju from 2012 to New tall fescue variety showed resistance to disease, persistence, and regrowth ability that were all enhanced when compared with Fawn. This study developed a new tall fescue variety with excellent environmental adaptability, aiming to make a contribution to the vitalization of the Korean grassland industry Acknowledgement: This work was carried out with the support of Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ012503). *Corresponding Author: Tel , kiwon@korea.kr 178

221 PB-86 함태호 1*, 권순욱 2, 류수노 본연구는기능성쌀인슈퍼홍미에함유된성분인 Taxifolin 과질소시비량, 등숙시기에따른함량을변화를조사하여환경변화에따른기능성성분의변화요인을밝히고자수행되었다. 만생종인슈퍼홍미벼를공시하고, 질소시비를 (5, 9, 13kg/10a) 하여 2016 년에시험포장에서수행한실험결과를요약하면, 당뇨억제성분인탁시폴린은슈퍼홍미의종실내의종피와과피부위에함유되었으며등숙시기별함량은출수후빠를수록높았으며출수후기간이지남에따라점점낮아졌다. 이는등숙이진행됨에따라 taxifolin 이포함되지않은배와배유부분이비대해짐으로상대적으로종피부분이낮아졌기때문으로판단된다. 슈퍼홍미는흑진주벼와수원 425 호교잡후대계통에서선발된 C3GHi 계통과종실이큰대립벼 1 호를인공교배한후대에서계통육종으로육성한품종이다. 출수기는 9 월 5 일로슈퍼자미보다 10 일늦은만생종이며, 간장은 94.7 cm 로슈퍼자미보다 13 cm 큰장간이다. 포기당이삭수는 5.4 개로적지만이삭당벼알수는 개로슈퍼자미보다 28% 많다. 현미의천립중은 26.8g 으로슈퍼자미와비슷하다. 슈퍼홍미의정조길이는 9.05 cm 이고폭은 3.79 cm 로슈퍼자미보다큰대립이며정현비율은 81.7% 이다. 기존의갈색유색미품종인건강홍미, 적진주, 홍진주와교배모본인흑진주, 수원 425 호, C3GHi 품종, 그리고같은교배모본으로육성된슈퍼자미, 슈퍼자미 2 호품종에유색미발현관련유전자마커와 SSR 마커를이용하여유전자특성을조사하였다. 유전자마커분석결과슈퍼홍미는기존에알려진갈색유색미관련유전자인 Rc, Rd 유전자가아닌다른유전자에의해적갈색이발현되고있으며, 흑자색유색미관련유전자인 Pb 유전자를가지고있는것으로조사되었다. * 주저자 : Tel , lion78@daum.net PB-87 정지웅 *, 정종민, 강경호, 고재권, 김보경 다양한돌려짓기체계개발을촉진하여농지활용도와농가소득을향상시키기위한목적으로 고시히카리 에대한돌연변이육종을통해극조숙품종인 백일미 가육성되었다. 백일미 는극조숙성과더불어도열병에민감한 고시히카리 보다매우안정적인저항성도함께겸비하고있음이확인되었다. 포장에서확보된 33 개의고병원성균주들에대한저항성평가 (0: 저항 5: 민감 ) 에서도 고시히카리 는 4 5(33 개균주평균 4.61) 의높은이병성을보인반면, 백일미 는 3 개균주 (88-031: 3, NC07-097: 3, NC14-096: 4) 를제외한모든균주에대해강한저항성 (0, 1) 을보였다 (33 개균주평균 0.64). 백일미 의극조숙에관여하는유전자를규명하기위해 고시히카리 와의교잡후대로부터 145 계통의 RIL 집단을육성하여 2 개의도열병균주 (NC14-020, NC14-054) 에대한반응기작을평가하였다. RIL 집단의저항성평균은각각 4.0(NC14-020) 과 3.0(NC14-054) 을보이며좌편향된분포양상을보였다. RIL 집단에대한유전분석을수행하기위해 Genotyping-by-Sequencing(GBS) 기법을활용, 총 311 개의유의한 SNP 를확보하여연관지도를작성하였다. Single Locus ANOVA 분석을통하여 RIL 계통들의 GBS 유전자형과표현형 ( 도열병균쥬에대한저항성 ) 간의관련성분석을실시한결과, 염색체 1 번말단 Mbp 영역에서두균주모두에대해안정적인저항성을발현하는유전자위를확인할수있었다. 해당유전자좌는 RIL 집단내표현형분산량의 32.5%(NC14-020) 와 51.2%(NC14-054) 를설명하는주동유전자였으며, 백일미 대립인자에의한저항성증가정도는 고시히카리 대비각각 1.3(NC14-020) 과 2.1(NC14-054) 이었다. 본연구를통해확인된 백일미 의변이유전자는향후벼픔종의도열병저항성을향상시키기위한분자육종재료로활용될수있을것으로판단된다. 사사 : 본연구는차세대바이오그린 21 사업 ( 과제번호 : PJ ) 의지원으로수행되었음 * 교신저자 : Tel , jrnj@korea.kr 179

222 PB-88 정지웅 *, 정종민, 김우재, 김현순, 김보경 농지활용도와농가소득향상을위한다양한돌려짓기체계의개발에활용코자 고시히카리 ( 출수기 8 월 3 일 ; 전주 ) 에대한돌연변이육종을통하여극조숙벼품종인 백일미 ( 출수기 7 월 15 일 ; 전주 ) 가육성된바있다. 백일미 의극조숙에관여하는유전자를규명하기위해 고시히카리 와의교잡후대로부터 145 계통의 RIL 집단을육성하고출수기분리양상을조사하였다. RIL 집단내에서출수기는 7 월 12 일 ( 파종후 64 일 ) 8 월 22 일 ( 파종후 105 일 ) 의범위에서파종후출수기까지의평균소요일수는 79.5 일로우편향된 (Skewness=0.82) 분포양상을보였다. RIL 집단에대한유전분석을수행하기위해 Genotyping-by-Sequencing(GBS) 기법을활용, 총 311 개의유의한 SNP 를확보하여연관지도를작성하였다. Single Locus ANOVA 분석을통하여 RIL 계통들의 GBS 유전자형과표현형 ( 파종후출수기까지소요일수 ) 간의관련성분석을실시한결과, 염색체 6 번 8.643Mbp 영역과염색체 7 번 Mbp 영역에서유의한유전자위들이확인되었다. 각유전자좌들은 RIL 집단내표현형분산량의 27%( 염색체 6 번 ) 와 28%( 염색체 7 번 ) 를각각설명할수있었으며, 백일미 대립인자에의한출수기단축효과는 고시히카리 대비 9.6 일 ( 염색체 6 번 ) 및 10.3 일 ( 염색체 7 번 ) 로각각추정되었다. 본연구를통해확인된 백일미 의두대립인자들은향후극조숙벼픔종육성을위한분자육종재료로활용될수있을것으로판단된다. 사사 : 본연구는차세대바이오그린 21 사업 ( 과제번호 : PJ ) 의지원으로수행되었음 * 교신저자 : Tel , jrnj@korea.kr PC-01 Mid-Eum Park 1, Kyeong-Ryeol Lee 2, Hyun Uk Kim 1* 1 2 Plants produce triacylglycerol (TAG) that is an ester between glycerol and three fatty acids in seeds. Most of the plants produce five common fatty acids that are composed of palmitic acid (16:0), stearic acid (18:0), oleic acid (18:1), linoleic acid (18:2), linolenic acid (18:3). However, some plants produce uncommon fatty acids that are useful for industrial application. Castor bean plant have a 80~90% of hydroxy fatty acid (ricinoleic acid, 18:1-OH) in seeds, but they also have a toxin ricin and allergenic 2S albumin in seeds. To produce hydroxy fatty acids (HFAs) in transgenic plants, many researchers have tried to increase HFA accumulation in Arabidopsis as a model oil plant using transformation of genes that are related to HFA synthesis from castor bean plant. Expression of RcFAH12 gene encoding a oleate Δ12-hydroxylase in Arabidopsis can accumulate HFAs up to 17% in seed oil. Coexpression of RcFAH12 and RcPDAT1-2 encoding a phospholipid:diacylglycerol acyltransferase can accumulate HFAs up to 22~23% in seed oil. Additional expression of RcDGAT2 encoding a diacylglycerol acyltransferase to RcFAH12+RcPDAT1-2 transgenic Arabidopsis increased HFA up to 27% in seed oil. Currently, we designed a pair of single guide RNA (sgrna) for gene editing and we cloned a CRISPR-Cas9 vector to knock-out genes encoding Arabidopsis PDAT and DGAT genes. We will eliminate the function of competitive endogenous genes that are related to TAG biosynthesis, thereby transgenic Arabidopsis will increase HFA more than current maximum 27% level. These result will contribute to produce better and more HFA in oil crops. In addition, this will help to understand lipid metabolism pathway. *Corresponding Author: Tel , hukim64@sejong.ac.kr 180

223 PC-02 Mi-Seon Kim, Bong-Sik Yu *, Jae-A Jung, Jong-Taek Park and Hak-Ki Shin 카네이션은우리나라화훼산업에서절화생산액이 131 억원 (2015, 화훼재배현황 ) 로비중이높지만, 종묘의자급률이매우낮다. 카네이션수입품종의묘값은품종에따라다르나, 평균주당 400 원대로비싸농가의경영부담이매우크다. 이러한어려움을해결하기위해국립원예특작과학원에서절화용카네이션 31 품종이개발하였다. 일부품종은농가에서재배하여시장출하를하고있으나수입품종의화색, 화형의다양성에는아직매우미흡하여다양한국산품종개발, 보급이요구되고있다. 따라서, 2011 년에화사한노랑색계의 GumByul 품종을모본, 노랑색바탕색에빨강색가는테두리가있는황색계 Picaro 품종을부본으로하여교배하였다. 실생계통양성및특성검정은 2012~2016 년에걸쳐 3 차의개화및생육을조사하여균일성등을확인하여최종적으로원교 B2-70 호계통을선발한후품평회를거쳐재배자, 유통업체등의기호도를평가하여 2016 년직무육성심의회를거쳐 Cream Magic 이라명명하여품종화하였다. 카네이션 Cream Magic 은절화용스프레이형으로베이지색바탕색에분홍색의줄무늬테두리가있는투톤의밝은화색이며개화가빠르고여름고온기적응성이우수하고생육이강건하며화형이둥글고꽃수가꽃대 1 개당 9.5 개로많아볼륨감이우수하다. 본품종의향기의정도를조사하기위해전자코 (Foxα 2000) 로분석한결과, 향기가강한 매리다 품종과비교시에도향기의정도가약간더강하게조사되어품종의우수성이더부각되었다. 더위에매우약한카네이션의특성상, 고온기하우스내온도가 30 이상 7 일이상재배시품질이떨어질우려가있으니주의가필요하다. * 교신저자 : Tel , kimms290@korea.go.kr PC-03 Yang Qin, Hee-Jong Woo, Kong-Sik Shin, Myung-Ho Lim, Hyun-Suk Cho, Seong-Kon Lee * With the development of a growing number of genetically modified (GM) crops, it is necessary to explore a detail, accurate and active molecular characterization method to select candidate events from a large pool of transformation events. Relative to traditional molecular analysis methods such as flanking sequencing and southern blot hybridization, the next generation sequencing (NGS) technology for whole genome sequencing of complex crop genomes had been proved to be useful for in-depth molecular characterization. In this study, five transformation events containing 1 Bt-resistant rice, 2 resveratrol-producing rices and 2 beta-carotene enhanced soybeans with one copy, two copies and three copies of foreign gene insertions respectively were conducted for southern blot hybridizations and NGS analysis, aim to reveal the relationship between copy number and read coverage. This finding might provide a useful way to confirm copy numbers of transformation events, consequently to cover the shortages of southern blot hybridization and flanking sequencing. *Corresponding Author: goryeong@korea.kr 181

224 PC-04 정용모 1*, 진영돈 1, 배민지, 최시림 1, 홍광표 2, 권오창 경상남도농업기술원화훼연구소에서, 기후변화에대응하여국내재배환경에적합하고거베라재배농가들의핑크색품종선택기회를더욱많이제공하며외국품종의로열티부담경감을위하여 2016 년화색이선명한핑크색절화용거베라 펄센스 를육성하였다 년 3 월핑크색대륜계의 핑크센세이션 을모본, 황색대륜계의 을부본으로인공교배를실시하여 4 월하순, 교배조합으로부터 364 개의종자를획득하였다. 교배후결실이된종자는 4 월하순경, 채종즉시파종하였으며, 그중 321 개의종자가발아 ( 발아율 88.2%) 하였다. 파종 1 개월후, 발아된유묘는본엽이 4 5 매전개되었을때화훼연구소유리온실내의선발포장에정식하였다. 정식후교잡 1 세대를육묘하여 2012 년 11 월하순경, 화색이선명하고수량이많은우수개체 ( ) 를 1 차로선발하였다. 그후특성이우수한 ( 경남교 G-58 호 ) 개체를 2012 년 12 월, 생장점배양에의해개체증식후조직배양묘의순화를거쳐서, 2013 년 10 월중순, 화훼연구소유리온실내의특성검정포장에정식하여 2016 년 7 월까지 3 회에걸쳐생육및수량특성검정을실시하였다. 특성검정을실시한결과화색, 화형, 수확량등품질과수량이우수하다고판단되어 2016 년농작물직무육성신품종심의회의심의를거쳐 펄센스 (Pearl Sense) 로명명하였다. 펄센스 품종의생육및개화특성조사를위하여대조품종으로 파티타임 (234259) 를사용하였다. 펄센스 품종은화색이선명한핑크색 (62D) 반겹꽃으로, 화폭이 12.5cm 정도인절화용대륜화이다. 또한포기당연간평균절화수량은 48.2 송이정도이며, 절화수명은약 12.4 일정도이다. 개화소요일수는 91.5 일로대비품종 파티타임 의 97.3 일에비하여약 6 일정도빠르며이때개화엽수는약 9.7 매정도이다. 펄센스 품종의설상화의길이와폭은각각 5.8m 와 0.8cm 로대조품종 파티타임 의 4.8cm, 0.9cm 와비교하여길이는 1.0cm 정도길었지만폭은다소작았다. 화경직경은상부와하부각각 0.6cm, 0.7cm 정도로대조품종 파티타임 의상부 0.6cm, 하부 0.8cm 와비슷한편이다. 재배상의유의사항은지온의관리및양 수분의흡수가쉽도록가능한이랑을높게만들고, 여름철고온기의생리장해및고온에의한꽃봉오리의유실방지를위하여차광재배하여온도상승을막아주고환기에주의하는것이좋다. * 주저자 : Tel , ymchung@korea.kr PC-05 Sais-Beul Lee 1, Tae-Heon Kim 1, Su-Min Jo 1, Jun-Hyun Cho 1, Ji-Yoon Lee 1, Jong-Hee Lee 2, Sang-Ik Han 1, You-Chun Song 1, Myung-Kyu Oh 1, Dong-Soo Park 1* 1 2 Bakanae disease is one of the most serious problems in rice production in Korea, and has become serious in the hybrid rice growing area, worldwide, where involves increased use of seed beds for the raising platelets. In this research, we used F 4 population to locate a QTL for resistance against bakanae disease. The 200 RILs (F 4) were derived from a cross between Wonseadaesoo and Junam. The previous reported SSR markers (for indica and japonica rice) could not represent the polymorphisms between the two japonica rice parents used in this study. To make the linkage map, then we developed 338 InDel markers based on the NGS (next generation sequencing) data of Wonseadaesoo and Junam. QTL analysis using 200 F 4 population detected a QTL, qbk WD, between InDel markers chr01_ and chr01_ , 1.59Mb interval, on chromosome 1 with a LOD score of 8.29 and the QTL explained 20.2 % of the total phenotype variation. we also identified Gene pyramiding effect of two QTLs, previously developed qbk1 and qbk WD, were also discussed. Results of this study are expected to provide useful information toward developing resistant rice lines harboring single or multiple major QTLs by pyramiding genes against bakanae disease. *Corresponding Author: Tel , parkds9709@korea.kr 182

225 PC-06 Youn Jung Choi *, Young Ran Lee, Su Young Lee, Yun Im Kang, Hae Ryong Cho Freesia (Freesia hybrida Hort.) is one of the popular ornamental crops over the world. Freesia Sunny Gold was developed for the cut flower in the National Institute of Horticultural Herbal Science in This hybrid was crossed and selected from a seedling of and Golden Flame in Morphological characteristics of the selected freesia hybrid were investigated for 5 years from 2011 to 2016, and then it was named Sunny Gold in Sweet Lemon has single flower and lemon petals (RHS, YO17B). The average flower width is 5.4 cm and the average yield is 6.3. The growth of the plant shows vigorous and the average height is 98.3cm, and it is higher than about 24 cm that of control cultivar Yvonne. The average number of floret per stalk was 10.3, and stalk was 6.9 cm length that of control cultivar Yvonne, 8.7 and 9.5cm length, respectively. The average days to first flowering of Sunny Gold, days, was approximately 8.7 days earlier than the control cultivar. It s average vase life and yield is 9.0 days and 5.3 cormlets per plant, respectively. The consumption of the yellow freesia such as Gold Rich, Yvonne, and Shiny Gold is approximately 80% in korean domestic cut flower market. Freesia Sunny Gold, as a mid-flowering freesia cultivar is expected to be popular to consumer. * 주저자 : Tel , lillium@korea.kr PC-07 Youn Jung Choi *, Young Ran Lee, Su Young Lee, Yun Im Kang, Hae Ryong Cho Freesia (Freesia hybrida Hort.) Love Fiction was developed for the cut flower in the National Institute of Horticultural Herbal Science in This hybrid was crossed and selected from a seedling of Golden Crown and White wing, with Teresa in Morphological characteristics of the selected freesia hybrid were investigated for 5 years from 2009 to 2014, and then it was named Love Fiction in Love Fiction has pink double petals (RHS, RPN74B). The average flower width is 6.6 cm and the average yield is 5.7. The growth of the plant shows vigorous and the average height is 101.7cm, and it is higher than about 34.6 cm that of control cultivar Opala. The average number of floret per stalk was 10, and stalk was 9.3 cm length that of control cultivar Opala, 11.7 and 10.5 cm length, respectively. The average days to first flowering of Love Fiction, days and the average vase life and yield is 9.3 days and 5.0 cormlets per plant, respectively. Despite the various colors of freesia flower, the consumption of the domestic cut-flower market is predominantly in the yellow freesia. This pink double freesia cultivar is expected to be useful for wedding bouquets not only in domestic market but also in overseas market. * 주저자 : Tel , lillium@korea.kr 183

226 PC-08 박석만 *, 박재호, 윤수현, 김민주 국내의감귤소비시장은 90% 가생과로소비되며 10% 정도가가공용으로이용되고있다. 이러한감귤생과시장에있어무핵여부는소비자의기호에많은영향을미친다. 국내에유통되는감귤은일부금감을제외하고는대부분이무핵품종이다. 그러나감귤품종육성기술이발달하고외국의감귤유전자원이되입됨에따라다양한교잡계통및품종이개발되고이들중에는우수한품질을가지고있으나종자가발생하는단점을가진계통이육성되기시작하였다. 이러한문제는국내뿐만아니라감귤육종프로그램을운영하는대부분의국가에서발생되고있으며이를극복하기위한다양한방법들이연구되고있다. 그중에한방법이방사선조사방법이며이러한방법을이용하여이스라엘, 호주, 스페인등에서는무핵의품종을육성한사례가있으며, 중국감귤연구소에서도이러한연구를수행하고있다. 본연구는 2012 년에육성된유핵의신예감을이용하여방사선조사를통해무핵의계통을육성하고자수행되었다 년 3 월신예감으로부터접수를채취하여제주대학교방사선연구원에서 60Gy 와 100Gy 의감마선을조사하였다. 이접수를그해 4 월탱자에접목을실시하였으며 1 년간생장을시켰다 년생장한접목묘로부터다시접수를채취하여 8 년생탱자에접목을실시하였다. 이들접목묘로부터 2016 년 1 월 12 일에 60Gy 조사계통 48 계통, 100Gy 22 계통의착과과실을수확하여과실특성및종자유무를확인하였다. 그결과각각 7 계통씩총 14 계통이무핵임을확인하였다. 무핵으로확인된계통을대상으로착화된 12 계통에대하여 2017 년 5 월에화기형태및꽃가루를관찰하여무핵의원인을규명하고자하였다. 화기형태관찰결과 계통의경우수술이매우작았으며현미경을통한화분수관찰에서정상보다매우적은화분의수를확인할수있었다. 이를통해이계통의경우웅성불임에의한무핵이유도되었을것으로생각된다. * 교신저자 : Tel , babau2000@korea.kr PC-09 Eun Ju Lee, Jun Young Choi, Sun-geum Jeong, Youn-Sung Kim * The CRISPR-Cas9 system, a novel molecular tool for site-specific genome modification, was recently introduced into plants by transient or stable transformation. In carrot, Agrobacterium tumefaciens-mediated transformation system is well established, but a gene targeting using CRISPR-Cas9 system is not. Chalcone synthase is a rate-limiting enzyme in the biosynthesis of anthocyanin that accumulates in plant organs such as flowers, fruits and roots. The purpose of this study is to produce the transgenic plants with inhibition of anthocyanin biosynthesis using A. tumefaciens-mediated CRISPR-Cas9 system in carrot. A. tumefaciens GV3101 harboring phatc containing genes encoding Cas9, DcCHS1 sgrna, and hygromycin phosphotransferase was used to infect hypocotyl explants. After 4 weeks of culture on selection medium, the explants produced embryogenic callus mainly at the cut edge. Plantlets were generated via embryo formation from the embryogenic callus after 8-12 weeks in culture. Under the selection with hygromycin, transgenic plants were selected and the insertion of transgenes into the transgenic plants was identified using PCR analysis. Targeted deep sequencing of transgenic plants revealed the deletion mutation at the target site. RT-PCR analysis showed the two bands, which may be obtained by the altered splicing. To confirm the root color of the transgenic plant, phenotype analysis was carried out, showing that the root color was changed from purple to yellow green. HPLC analysis showed that cyanidin was not detected in the transgenic line. These results showed that gene editing with CRISPR-Cas9 system in carrot could be useful for the development of the new valuable varieties. *Corresponding Author: Tel , yskim0907@naver.com 184

227 PC-10 조신환, 안율균 *, 김진희, 이은수, 김정호, 김도선 박이나오이, 멜론, 수박과같은박과채소작물을재배할경우물이나온도등의영향을받아생산량이감소하게되어, 이에대한저항성을가진작물육종이필요하다. 우리나라나일본등몇몇나라에서는이런불리한환경을극복하기위해박을대목으로하여접목재배를하고있으며, 최근 EU 에서는형질전환체를대목으로하여접목하는기술을 NBT (New Breeding Technology) 중하나로정의하였다. 이연구에서는저온적응과관련된 transcription factor 중하나인 CBF3 가도입된박 (Lageneraria siceraria Standl.) 을대목으로하여, 시판중인수박을접목한후저온저항성이있는지확인하였다. 접목한식물을저온처리한결과 3 일차에서부터점점식물이스트레스를받는것을육안으로확인할수있었으며, 4 일차에서비 - 형질전환체를대목으로한식물체에서고사직전까지의모습을보이기도하였다. 특히저온처리시수박의무게에서급격한감소가나타났다. 비 - 형질전환체를대목으로식물체의수박은 3 일차에서 1001 g, 4 일차에서 388 g 으로눈에띄게감소하였지만, 형질전환체를대목으로한식물체에서는 3 일차에서 1266 g, 4 일차에서 1237 g 으로관찰되어큰차이를보이지않았다. 따라서 CBF3 형질전환체를대목으로사용할경우작물의생장이나생산량증대에많은효과를줄수있을것으로기대된다. * 교신저자 : Tel , aykyun@korea.kr PC-11 조신환, 안율균 *, 김진희, 이은수, 김정호, 김도선 식물은생장함에있어서생물학적, 비생물학적스트레스의영향을받는다. 이러한스트레스에대한저항성을증진시키기위한많은노력중하나로저항성을가진두종류이상의유전자를집적시키는방법이있다. 한개의도입유전자를가진형질전환체를서로교배를통해두가지외부유전자를가진식물체를얻어복합저항성식물체를개발할수있다. 이연구에서는 H+-pyrophosphatase 를코딩하는애기장대 AVP1 유전자를도입시킨형질전환체와 CBF3 형질전환체를교배하여 AVP1 과 CBF3 유전자집적계통을획득하였으며저온저항성을확인하였다. 저온처리결과비 - 형질전환체와 AVP1 형질전환체는거의고사하는모습을보였지만, CBF3 형질전환체와교잡식물체는살아있는것을육안으로확인할수있었으며, 생육조사결과식물체의키와잎의넓이및길이, 개수에서차이를보였다. 이는 CBF3 형질전환체와교배를통해 AVP1 형질전환체가저온저항성을나타내게된것이다. 따라서교배를통해두가지이상의유전자가집적하게된다면복합저항성을가진식물체를개발할수있으며, 작물의생장이나생산량증대효과를가져와농가소득향상에기여할수있을것이다. * 교신저자 : Tel , aykyun@korea.kr 185

228 PC-12 진영돈 1*, 안동춘 1, 배민지 1, 정용모 1, 황주천 1, 최시림 1, 홍광표 경상남도농업기술원화훼연구소에서는분화용국화로열티부담경감과생산비절감을위하여 2016 년핑크색홑꽃조기개화성품종인 가야베이비 를육성하였다 년 1 월에성장세가균일하고개화가빠른핑크색 하모니핑크 를모본, 흰녹병에강하며개화가빠른핑크색 핑크아이 (CFC0087) 를부본으로인공교배하여교배조합으로부터 180 개의종자를획득하였다. 채종즉시파종하고발아시켜 1 개월정도육묘한후 10 cm화분에정식하고 3 주재배후적심하고단일처리하여개화가빠르고화색이우수한 HPPI-10, 23, 57, 110 등 4 계통을우수개체로선발하였다. 선발된 4 계통을 2014 년부터 2015 년까지 2 년간에걸쳐 1 2 차생육특성검정을통해기호성, 안정성, 균일성과흰녹병저항성등을조사하여최종적으로 HPPI-23 을선발하였다 년에계통번호 경남교 CP-50 호 를부여하여 3 차특성검정을수행해생육과개화특성, 안정성과균일성, 품평회와시장출하등을통해생산자와소비자의기호성평가를받았다. 그결과시장성과기호성이좋고, 화색및화형등품질이우수하다고판단되어 2016 년농작물직무육성신품종심의회심의를거쳐 가야베이비 으로명명하고국립종자원에품종보호출원하였다. 가야베이비 는연핑크색 (P75B) 홑꽃화형으로자연일장에서는 10 월 25 일경에개화하는추국이며, 촉성및억제재배에서단일처리후개화소요일이 5.5 주이하로조기개화하는품종이다. 꽃크기는 4.0cm 로중형이며암적색의화심을가지고있으며평균개화소요일이 36 일로짧다. 착화수와분지수가 26.4 개와 5.2 개로많아상품성이좋으며흰녹병에강하고재배기간이짧아상품성이좋은품종이다. 화훼연구소에서개발된 가야시리즈 와동시출하시상품성이높을것으로보이며 10cm 내외의화분에적심후에일반적인분화국화재배법으로재배하면휴면성이약해연중생산이가능하다. 재배상의유의사항은여름철고온기의화색퇴화및개화지연방지를위하여차광을해주고서늘하게관리하는것이좋다. * 주저자 : Tel , ydchin@korea.kr PC-13 박은준 *, 양은영, 채수영, 문지혜, 박태성, 김옥례, 김대영, 이선이, 김상규 다수성, 고품질과더불어생물학적또는비생물학적스트레스에도저항성이있는새로운품종을개발하기위한다양한육종기술이요구되고있다. 반수체와배가반수체는새로운육종방법의하나인반수체육종의좋은재료가될수있다. 고추에서반수체 / 배가반수체를생산하는방법으로약배양, shed- 소포자배양, 나출소포자배양등이보고되고있다. 현재까지실제육종에이용되고있는방법은약배양으로각회사및연구소에서자체적으로기술을보유하고있다. 그러나최근소포자배양이가능해지면서약배양보다높은효율로배를생산할수있는배양방법들이보고되고있다. shed- 소포자배양기술은고체배지와액체배지를동시에이용하는변형된약배양으로고추에서다수의배를확보할수있는방법이다. 본연구에서는고추의 shed- 소포자배양기술확립을위해전처리온도, 배양배지의조성과탄소원의종류및농도가소포자배발생에미치는영향을조사하였다. 전처리가소포자배발생에미치는영향을조사한결과 9 에서 7 일동안저온처리후 Maltose 가 8% 포함된배지에서배양시 5.4 개의배가발생하였다. 배양배지조성과탄소원에따른배발생효율을조사하기위해 maltose 와 sucrose 가포함된 1/2NLN, NLN, NN, MS2 배지를사용한결과 NLN 배지에서가장많은소포자배가발생하였으며, 탄소원의종류에따른차이는크지않았다. 탄소원농도의영향을조사하기위해액체배지내 sucrose 를 2, 4, 6, 8, 10% 농도로첨가후 28 에서배양한결과소포자배발생에영향을미치는최적의 sucrose 농도에따른차이는크지않았으나밀양재래는 6%, long fruit 품종은 6, 10% 에서배발생이높았다. 본연구결과들은 shed- 소포자배양을통해반수체 / 배가반수체를생산하기위한연구의기초자료로이용될수있을것이다. * 주저자 : Tel , ejpark97@korea.kr 186

229 PC-14 이상협 1*, 황병호 브로콜리는항암작용을비롯하여, 고혈압및심혈관질환에대한예방효과와해독효소의유도효과가크다고알려져있다. 따라서 2000 년이후국내소비량이매년상당히증가되고있는실정이다. 브로콜리의대표적인유효성분은 sulforaphane(s-methylsulfinylbutyl isothiocyanate) 로알려져있고, 세포조직이마쇄될때전구물질인 glucoraphanin 이 myrosinase 에의해분해되면서 sulforaphane nitrile 과함께생성된다고알려져있다. 따라서 glucoraphanin 고함량브로콜리육성은브로콜리육성기술을한단계높이는계기가될것이다 년미국몬산토사에서 Glucoraphanin 함량이기존재배종의 2~3 배되는 Beneforte 라는품종을전통육종을통해출시하였다. vilosa 라는야생자원 (Glucoraphanin 함량이재배종의 2~3 배 ) 을십수년동안 back-crossing 한결과이다. 본연구에서는 CRISP/Cas9 이라는분자생물학기법을적용하여 Glucorapanin 함량을높이고자하는시도를추구한다. Acknowledgement: This work was supported by the Bio-industry Technology Development Program [grant number , ] of ipet (Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry) * 주저자 : Tel , sanglee@sejong.ac.kr PC-15 이진석 1*, 손범영 1, 김정태 1, 배환희 1, 김상곤 1, 백성범 1 1 국내옥수수반수체유기계통을개발하기위하여열대형반수체유기계통 (TAIL, Tropically Adapted Inducer Line) 의특성및유기된반수체옥수수의특성평가를수행하였다. TAIL P1 과 TAIL P1/P2 는생물학적마커로이용하기위하여제 1 엽의엽초에안토시아닌색소를발현하였고출사일수는모두 71 일이었으며화분비산후출사까지의기간이 1~2 일이었다. TAIL 의반수체유기율을평가하기위하여광평옥, SK165, KS124, KS164, KY48 등을검정친으로하여 TAIL P1 과교배를수행하였으며 R1-nj 유전자의발현에따라반수체유기율을평가한결과 R1-nj 유전자발현에의한반수체유기율은 10.5% 였다. 이들을 50 공육묘포트에모두파종한후제 1 엽엽초의안토시아닌색소발현을확인하였다. R1-nj 유전자발현에의해이배체로선발된종자중 2% 는반수체종자였고반수체로선발된종자중 47.9% 는이배체종자였으며실제반수체유기율은 7.8% 로 R1-nj 유전자발현에의한유기율과는차이가있었다. 이배체와반수체식물체를포장에이식하여반수체옥수수와생육특성을비교한결과, 반수체옥수수는간장, 엽폭, 간경등이이배체옥수수보다작았으며출웅과출사는정상적으로이루어졌으나화분은형성되지않았다. 사사 : 본연구는농촌진흥청차세대바이오그린 21 연구사업 ( 세부과제명 : 옥수수반수체유기유전자도입및국내적응자원개발, 세부과제번호 : PJ ) 의지원에의해이루어진것이다. * 주저자 : Tel , z @korea.kr 187

230 PC-16 박민영 1,2, 장하영 1, 임용표 2, 이정수 1, 박수형 1* 1 2 십자화과채소에서소포자배양은 doubled-haploid (DH) 계통을개발하는데널리사용된다. 육종가들은유전적으로동일한상동염색체를갖는 DH 계통을양친으로사용하기를선호하며소포자배양기술을이용한육종은단기간에선택된식물재료로부터 DH 계통을개발할수있다는점에서유용하다. 국립원예특작과학원에서는유전적으로다양한양배추자원들의소포자배양을통하여효과적인시기와조건을구명하기위해본연구를실시하였다. 그결과, 배지조건의경우 2 NLN, AgNO 3 1mg/L, 13 % Sucrose 의조성이배생산에효과적임을확인할수있었다. 그리고배양재료로사용된다양한세대의양배추자원중소포자로부터유도된배발생효율은일대잡종세대보다자가수정후대인 F 2 또는 F 3 세대에서높았다. 이러한결과를통하여양배추의소포자배양시, 목적하는양배추자원으로부터유도된배의개수가일대잡종품종에서계획한양만큼도달하지못했을경우, 그의자손세대를사용하여소포자배양을지속하는것을추천한다. 그리고본연구를통하여획득된배발생개수와토양순화개체수는소포자배양전체기간중, 개화초기의결과가중기및말기보다높았다. 토양순화의경우, 개화초기에수집한꽃봉오리를이용하여소포자배양한결과에서식물체로토양순화된비율이전체중 71.2% 로중기와말기의각각 27.0%, 1.8% 결과보다월등히높았다. 이러한결과를통하여양배추의소포자배양을하고자할때, 저온처리후꽃봉오리가발생되는전체기간동안배양을진행하는것보다, 짧게는개화초기 ( 첫개화후 14 일까지 ) 에만, 늦어도개화중기의초기까지배양을진행하는것이배양성공면이나, 노동력절감면에서더효율적이라고판단된다. * 교신저자 : psh@korea.kr PC-17 류시환 *, 최재근, 박종열, 남궁민, 용우식, 윤석원, 박기진, 최준근 우량계통육종은옥수수교잡종육성을위한필수요소이다. 국내의옥수수계통육종은순도고정을위해 7 회이상의인공교배 (selfing) 를수행하는전통적인방법에의존하고있다. 시간과노력소모가많은전통육종의단점을극복하고자강원도농업기술원옥수수연구소에서는국제옥수수 밀연구소 (CIMMYT) 와협력하여배가반수체기술의국내도입에노력한결과, 국내최초로신기술을이용한옥수수계통육성에성공하였다. 배가반수체기술에의한옥수수계통육성체계는반수체유기, 염색체배가및계통생산, 그리고계통의특성평가등의 3 단계로이루어진다 년반수체유기체 (Inducer) 와의교배를통한반수체유기에서종실용옥수수 32P75 F2 집단과찰옥수수미백 2 호 F1 집단의반수체유기율은각각 4.0% 와 2.9% 였다 년 colchicine 를이용한염색체배가및계통생산에서 32P75 와미백 2 호집단으로부터각각 9 계통과 38 계통을생산하였다 년각집단에서육성된계통의표현형적특성평가를실시하여적응성, 도복, 내병성등이우수한 DHF1 및 DHW1 을선발하였다. DHF1 은출사일수 74 일, 간장 175cm, 100 립중 32g 의직립형종실용계통이다. DHW1 은출사일수 69 일, 착수고율 40%, 이삭장 11.5cm 의조숙성찰옥수수계통이다. 육성된계통은기존의엘리트계통과의교배를통해조합능력및생산력검정을수행할예정이며, 우량교잡종품종육성에활용성이높을것으로기대된다. 사사 : 본연구는농촌진흥청차세대바이오그린 21 사업 (PJ ) 의지원에의해이루어진것임 * 교신저자 : Tel , shr8921@korea.kr 188

231 PC-18 Jae-Ryoung Park, Won-Tae Yang, Doh-Hoon Kim, Yong-Sham Kwon * The assessment of genetic diversity investigated with SSR markers would benefit to make use of these germplasms as well as applying them in association mapping. The present study was conduct to evaluate the genetic diversity of rice germplasms with 376 black purple and 172 red pericarp color conserved by Dong-A university. The 600 pairs of simple sequence repeat (SSR) primers screened against 11 rice varieties. Sixteen informative primer pairs with high polymorphism information contents (PIC) value were used to assess genetic diversity in the collection. A total of 409 polymorphic amplified fragments were obtained by using 16 SSR markers. The number of alleles per locus ranged from 11 to 47 with an average of 25.6 alleles per locus. The average PIC was ranging from to Four hundred nine three SSR loci were used to calculate Jaccard s distance coefficients for unweighted pair group method with arithmetic mean cluster analysis. These accessions were separated into several distinctive groups corresponding to morphological types. At present, we are analyzing population structure and the relationship between molecular markers and major agronomic traits such as seed and major morphological trait in 548 rice accessions. These results would provide valuable information for colored rice breeding program and the protection of important germplasm resources using molecular marker. Acknowledgement: This work was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries(IPET) through Agri-Bio industry Technology Development Program, funded by Ministry of Agriculture, Food and Rural Affairs(MAFRA)( SB020) *Corresponding Author: Tel , yskwon3@dau.ac.kr PC-19 MyoungHee Lee *, JiMyoung Choi, EunYoung Oh, SungUp Kim, JungIn Kim, SukBok Pae, KiWon Oh, UnSang Yeo, DoYeon Kwak The perilla oil is a Korean traditional oil, it has over 60% proportion of omega-3 among fatty acids, which is the highest percentage among vegetable oils. The perilla oil were studied on the changing properties according to storage condition and fatty acid composition after oil extraction. Perilla seef was roasted at 170, extracted with oil press and stored at 4, 10, 250. Their lipid peroxidation was monitored by the acid and peroxide value test method every month. We analyzed the composition of fatty acid in perilla varieties and germplasm for examine oxidative stability according to α -linolenic acid composition. The acid value and peroxide value of perilla oil at 25 showed the increasing tendency in all the samples during the storage period. Though just a small changes was observed in the compositions of fatty acid but it was not affected seriously according to storage temperature and period. *Corresponding Author: Tel , emhee@korea.kr 189

232 PC-20 Shahina Akter 1, Hyo-Ju Lee 1, Ye Rim Lee 1, Yeo Jin Jung 1, Jong Hee Kim 1, Yong-Gu Cho 3, Kwon-Kyoo Kang 1,2, Yu-Jin Jung 1,2* Genome modification technologies such as ZFN (Zinc Finger Nuclease), TALEN (Transcription Activator-Like Effector Nuclease) and CRISPR/Cas9 technology have been successfully applied to improve various crop traits. Among them, CRISPR/Cas9 system is now conveniently available as a precise and efficient genome engineering tool. Targeted mutagenesis using CRISPR/Cas9 system is especially useful for plant breeding and gene functional analysis. In this study, we applied the CRISPR/Cas9 system to modify seed colors in higher plants using rice. Binary vectors harboring expression cassettes of Cas9 nuclease, single guide RNA (sgrna) targeting the Leucoanthocyanidin dioxygenase (LDOX) and bar as a selectable marker gene were constructed and used for production of transgenic rice plants via Agrobacterium-mediated transformation. Targeted mutations were analyzed in bar-resistant shoots by sequencing of the LDOX amplicons (PCR products) using NGS analysis. Transgenic rice plants clearly contained DNA mutations such as nucleotide substitutions, insertions and deletions at the target site, which varied depending on the transgenic lines. In the future, we will investigate seed color in transgenic lines. And these data will be discussing relationship between seed-color phenotypes and mutations in transgenic plants. *Corresponding Author: yuyu1216@hknu.ac.kr, kykang@hknu.ac.kr PC-21 Hyo-Ju Lee 1, Sangsu Bae 3, Geung-Joo Lee 4, Pil Joon Seo 5, Dong Hyun Kim 1, Jae Uk Lee 1, Yong-Gu Cho 6, Kwon Kyoo Kang 1,2*, Yu Jin Jung 1,2* The CRISPR/Cas9 is a core technology that can result in a paradigm for breeding new varieties. This study describes in detail the sgrna design, vector construction, and the development of a transgenic plant and its molecular analysis, and demonstrates how gene editing technology through the CRISPR/Cas9 system can be applied easily and accurately. CRISPR/Cas9 facilitates targeted gene editing through RNA-guided DNA cleavage, followed by cellular DNA repair mechanisms that introduce sequence changes at the site of cleavage. It also allows the generation of heritable- targeted gene mutations and corrections. Here, we present detailed procedures involved in the CRISPR/Cas9 system to acquire faster, easier and more cost-efficient gene edited transgenic rice. The protocol described here establishes the strategies and steps for the selection of targets, design of sgrna, vector construction, and analysis of the transgenic lines. The same principles can be used to customize the versatile CRISPR/Cas9 system, for application to other plant species. *Corresponding Author: yuyu1216@hknu.ac.kr, kykang@hknu.ac.kr 190

233 PC-22 김남훈 1, 김혜식 1, 신명주 1, 이현오 1, 최범순 1, 유의수 1* 1 작물의유전체정보생산은차세대유전체분석방법의발달로급속히증가하고있으며, 대량의유전변이데이터를확보하고이들정보의효율적인활용으로작물에서유전체육종을구현하기위한단계로전환하고있다. 따라서많은유전체정보중분자육종에적극적으로이용할수있는데이터베이스의구축과효율적이고통합된정보를추출, 활용이가능하게하는인터페이스의구축은분자육종의효율향상에많은기여를할것으로판단된다. 본연구는 육종현장에서사용이가능한유전체육종지원시스템의개발 의일환으로콩표준유전체를기반으로유전체재분석기술을이용한 NGS 데이터의분석을통해서국내보유콩유전자원내에존재하는 SNPs, indels, MIPs (MITE insertion polymorphism; MITEs=Miniature Inverted-repeat Transposable Elements) 등유전변이를확보하고질적, 양적형질의동정및개체선발에적용하기위한다량의분자마커를개발을위한분자마커생산모듈의개발에있다. MITE 기반의마커개발과정, 콩핵심집단대상대량의 SNP 및 indel 마커개발방법등의결과, 가시화전략등이본발표에소개되며, 과제의연구결과는관련작물유전체정보의집중화에기여하고, 정보관리, 분석방법에표준화를제시하여관련유전체정보의이용과해석에신뢰성을더하고, 분자육종시필요한 polymorphic 한다량의마커제작에도움을주어분자육종의효율을높일것으로기대한다. * 교신저자 : Tel , yeisooyu@phyzen.com PC-23 Jin Sun Kim *1, Su-Jin Choi 1, You-Ran Jang 1, Sun-Hyung Lim 1, Jong-Yeol Lee 1 1 By establishing a system that can efficiently induce transgenic plants using plant tissue culture and molecular biology techniques, it can be applied to wheat to produce crops with useful traits. But wheat is considered a plant that is recalcitrant to genetically modify because of its low transgenic efficiency and genotype dependency. Therefore, various wheat transformation methods are underway to increase efficiency. So far, studies of transformation methods based on the characteristics of Korean wheat cultivars have been reported mainly on the regeneration efficiency and the confirmation of transient expression in embryogenic callus, and studies of systematic transformation method have rarely been progressed. Therefore, the aim of this study was to establish transgenic plants in Korean wheat cultivar Keumkang which try to introduce the centrifuges and embryo axis excision as well as the conventional methods to increase the transformation efficiency by biolastic method. Immature embryos (IEs) between the 13th and 23th days of DPA were dissected and used for transformation. A total of 9,030 IEs were tested with gold particle bombardment to introduce the GFP gene. The phosphinothricin (ppt) resistance test, the incidence of callus and the rate of green spot occurrence in Keumkang wheat were checked according to whether embryo axis was removed or not. Based on results of this study, it will provide valuable information for further development and commercialization of transgenic wheat. *Presenting Author: Tel , jskim220@korea.kr 191

234 PC-24 박미소 1, 김남훈 1, 이승욱 1, 유의수 1, 강상호 2, 최범순 1* 1 2 결명자 (Senna tora L.) 는콩과에속하는일년생초본이다. 눈을맑게하는씨앗이란이름대로예로부터한의학에서시신경보호, 혈압강하, 항균, 이뇨등의치료에사용되어왔다. 결명자는 anthraquinone, flavonoide 등의이차대사산물을많이함유하고있고, 최근에는 emodin 성분이항암등에효능이있다고알려져산업적으로도가치가큰식물이다. 결명자의유전체크기는 600Mb 정도로알려져있으나, 아직까지결명자의유전체분석이나의학적효용이있는유전자들의기능및 pathway 연구는거의진행되지않은상태이다. NGS 기술의발전은수많은신규유전체분석을가능하게하고있다. 주식회사파이젠은 Illumina 사의 HiSeq 플랫폼기반의짧은염기서열을 de novo assembly 에이용한뒤생성되는부정확한 scaffold 의오류를줄이고자하였다. 그방안으로완성된유전체서열에 mate pair 데이터를다시붙여봄으로써 span size 를확인하는 Assembly Verification using Mate Pair (AVMP) 프로그램을파이젠유전체연구소에서개발하였다. AVMP (Assembly Verification using Mate Pair) 프로그램은 perl 과 GD library 2.46 을이용하여개발되었으며, 이는결명자유전체검증뿐만아니라다양한종에서유전체정보를검증함에있어많은기여를할것이라생각된다. * 주저자 : Tel , iamparkmiso@phyzen.com PC-25 Ji-Hye Lee, Jung-Hee Jang, Byoung-Rourl Choi and Tae-Jin Won * Gawaji 1, an early maturing and low amylose content rice variety adaptable to double cropping system with barley as later winter crop, was developed by the crop breeding team of Crop Research Division, GARES, Hwaseong, Korea in Gawaji 1 was derived from a cross between Baegjinju and Sangmibyeo in This variety had heading date of August 4 in Gyeonggi Province. It had a semi-elect plant type and resistance to lodging with strong culm. Its culm length was 67 cm. This variety had 15 tillers per hill and 104 spikelets per panicle. It was medium grain variety showing 1,000 grain weight of brown rice of 19.3g. Its protein contents of milled rice was 8.2% which was lower than Baegjinju. Its milling ratio was 75.5% which was higher than Baegjinju. The yield of milled rice was 5.0 MT/ha under the ordinary culture of the local adaptability test in 3 areas of Gyeonggi Province for three years. Gawaji1 is highly adaptable to Gyeonggi Province, especially to northern region of Han River. *Corresponding Author: Tel , wonboy@gg.go.kr 192

235 PC-26 Seong-Im Park 1, Young-Saeng Kim 1, Jin-Ju Kim 1, Il-Sup Kim 2, Ho-Sung Yoon 1* 1 2 Reactive oxygen species, which increase under various environmental stresses, have deleterious effects on plants. An important antioxidant, glutathione, is used to detoxify reactive oxygen species in plant cells and is mainly produced by two enzymes: gamma-glutamylcysteine synthetase (γ -ECS) and glutathione synthetase (GS). To evaluate the functional roles of the glutathione synthetase gene (OsGS) in rice, we generated four independent transgenic rice plants (TG1 TG4) that overexpressed OsGS under the control of the constitutively expressed OsCc1 promoter. When grown under natural paddy field conditions, the TG rice plants exhibited greater growth development, higher chlorophyll content, and higher GSH/GSSH ratios than control wild-type (WT) rice plants. Subsequently, the TG rice plants enhanced redox homeostasis by preventing hydroperoxide-mediated membrane damage, which improved their adaptation to environmental stresses. As a result, TG rice plants improved rice grain yield and total biomass following increases in panicle number and number of spikelets per panicle, despite differences in climate during the cultivation periods of 2014 and Overall, our results indicate that OsGS overexpression improved redox homeostasis by enhancing the glutathione pool, which resulted in greater tolerance to environmental stresses in the paddy fields. *Corresponding Author: Tel , hys@knu.ac.kr PC-27 Gyu Tae Park 1, Jagadeesh Sundaramoorthy 1, Seokhyung Lee 1, Jeong-Dong Lee 1, Hak Soo Seo 2, Jong Tae Song 1* 1 2 In nature, the wild relatives of soybean [Glycine soja Sieb. and Zucc.] have curly/wavy branches whereas cultivated varieties are upright. To investigate the molecular mechanism of coiling nature, screening was carried out from Arabidopsis activation tagging lines obtained by activation T-DNA treatment that have curly/wavy morphology. The mutant with a wavy and curly morphology with coiling branches was named cbr. Plasmid rescue and genomic southern blot analysis revealed the site of T-DNA insertion in the genome. RT-PCR was performed to evaluate the expression levels of the genes adjacent to the T-DNA integration site and showed the activation of an E3 ubiquitin ligase gene. Database search revealed that the protein with the C3HC4 type RING domain belongs to a family of E3 ubiquitin ligases. Complementation test by overexpression and RNA interference of the gene showed that activation of the novel gene caused the cbr mutant phenotypes. Ubiquitylation has been linked virtually to every cellular process including plant development. E3 ubiquitin ligase has been reported to recognize target proteins that are to be ubiquinated for further degradation by the proteasome complex. Therefore, we are performing 2-DE and Y2H experiments to find specific substrate(s) of the novel E3 ubiquitin ligase gene. Acknowledgement: This work was carried out with the support of Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ ) Rural Development Administration, Republic of Korea. *Corresponding Author: Tel , jtsong68@knu.ac.kr

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237 식물분자육종사업단 & GM 작물개발사업단 2017년 7월 7일 ( 금 ), 09:00 ~ 10:40 대구엑스코 식물분자육종사업단 & GM작물개발사업단

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239 OF-01 In Hye Lee 1, Yu-Jin Jung 1,2, Yong Gu Cho 3, Ill Sup Nou 4, Md. Amdadul Huq 1,2, Franz Marielle Nogoy 3, Kwon-Kyoo Kang 1,2* As rice is the main food source for more than half of the world s population, fluctuation in production can indirectly make a significant impact on the world economy. Bacterial leaf blight and blast are the most important diseases affecting rice production. They are difficult to control because the speed of diffusion is very fast. The usual way to control such type of pathogens is to spray pesticides. However, continuous and extensive use of such pesticides causes longterm exposure of the environment to contamination. In addition, many microorganisms can acquire resistance to chemical pesticides [Daoubi et. al., 2005]. The most effective and environment friendly way to control bacterial leaf blight and blast diseases is deployment of resistant cultivars. Classical plant breeding for resistant varieties requires complex system development like identification of source for donor genes, time and cost of breeding, stability of resistance and multiple location tests. Biotechnology can be used to complement the shortcomings of traditional breeding, and a number of success stories have already been reported. Antimicrobial peptides (AMPs) are components of the defense system of animals and plants against pathogens and are generally shorter than 50 amino acid residues per peptide. AMPs are controlled by a single gene, and can destroy microorganisms, including bacteria, fungi, mycoplasma, and viruses, with minimum energy consumption during the initial infection. Because of this huge advantage, scientists used AMPs as one of the important sources for breeding resistant varieties [Rahnamaeian M, 2011]. Human LL-37 is a multifunctional antimicrobial peptide of cathelicidin family. It has been shown in recent studies that it can serve as a host s defense against influenza A virus. We now demonstrate in this study how signal peptide LL-37 (SP-LL-37) can be used in rice resistance against bacterial leaf blight and blast. We synthesized LL-37 peptide and subcloned in a recombinant ppzp vector with pgd1 as promoter. SP-LL-37 was introduced into rice plants by Agrobacterium mediated transformation. Stable expression of SP-LL-37 in transgenic rice plants was confirmed by RT-PCR and ELISA analyses. Subcellular localization of SP-LL-37-GFP fusion protein showed evidently in intercellular space. Our data on testing for resistance to bacterial leaf blight and blast revealed that the transgenic lines are highly resistant compared to its wildtype. Our results suggest that LL-37 can be further explored to improve wide-spectrum resistance to biotic stress in rice. *Corresponding Author: Tel , kykang@hknu.ac.kr OF-02 Soon Ju Park 1*, Sebastian Soyk 2, Min-Sung Kang 1, Zachary B Lippman Tomato life adaptation in a year and sympodial determination fitness are the most important factors to increase yield for fresh market and commercial products. Here we show that tomato productivity can be fine-tuned and optimized by exploiting combinations of selected mutations in multiple florigen pathway components. By screening for chemically induced mutations that suppress the bushy, determinate growth habit of field tomatoes, we isolated a new weak allele of the florigen gene SINGLE FLOWER TRUSS (SFT) and two mutations affecting a bzip transcription factor component of the florigen activation complex. By combining heterozygous mutations, we pinpointed an optimal balance of flowering signals, resulting in a new partially determinate architecture that translated to maximum yields. Additionally, we further show that loss of day-length-sensitive flowering in tomato was driven by the florigen paralog and flowering repressor SELF-PRUNING 5G (SP5G). SP5G expression is induced to high levels during long days in wild species, but not in cultivated tomato because of cis-regulatory variation. CRISPR/Cas9-engineered mutations in SP5G cause rapid flowering and enhance the compact determinate growth habit of field tomatoes, resulting in a quick burst of flower production that translates to an early yield. We propose that harnessing mutations in the florigen pathway to customize plant architecture and flower production offers a broad toolkit to boost crop productivity. *Corresponding Author: sjpark75@wku.ac.kr 195

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241 농생물게놈활용연구사업단 2017년 7월 7일 ( 금 ), 09:00 ~ 10:40 대구엑스코 농생물게놈활용연구사업단

242 Concurrent Session( ) : 농생물게놈활용연구사업단 시간발표제목 ( 가제 ) 발표자 ( 소속 ) 각발표는 20 분, 질의응답 5 분씩배정 198

243 OG-01 Kyu-Won Kim 1, Qiang He 2, Win Htet Oo 2, Ji-Min Yoo 2, Phitaktansakul Rungnapa 2, Dong-Hee Kim 1, Jung-Rye Nam 1, Sang-Ho Chu 1, Wei-Guo Zhao 3, Sang-Won Park 4, Il-Pyung Ahn 5, Yong-Soo Choi 6, Yoo-Hyun Cho 7, Young-Sang Lee 8, Jung-Kyung Moon 9, Yong-Jin Park 1,2* The development of high-throughput technology has led to a boost in omic-wide studies on organisms, and it is now actively applied to crop breeding. For domestic rice, we have produced and analyzed genome, transcriptome, and proteome from the Korean rice core collection (KRICE_CORE). Using genomic data, we characterized the ecotypes of Korean wild rice and identified the evolutionary characteristics of weed rice to evaluate the values of Korean wild and weed rice as genetic resources. We also carried out genome-, transcriptome-, proteome-wide association studies on major agricultural traits of rice. We applied a set of bioinformatics techniques for precise identification of candidate genes from the omic data. Our study showed the possibilities for using omic data in rice breeding. *Corresponding Author: yjpark@kongju.ac.kr OG-02 Man-Soo Choi A core collection is small population to represent the genetic diversity of the entire collection in plant germplasm resources, and is important strategy available as a small part of a germplasm collection to represent of the entire collection. Especially, developing of the core collection in soybean is also important and valuable task as one of the most important crop resources worldwide. Here, we developed the core collection consisting of 816 accessions by using approximately 180,000 (180K) single nucleotide polymorphisms (SNPs) developed in previous study. In addition, we performed genetic diversity and population structure analysis to construct the core collection from entire 4,400 collections. A good core collection has to conserve as much variation, such as phenotypic or genotypic, has no redundant accession included in the core, and is small enough to be easily manageable size. Therefore, there were excluded sample call rates less than 97% and also were removed duplicate samples more than 99.9% according to genotype analysis using 180K SNPs from total collections. Furthermore, we were also excluded natural hybridization resources of Glycine max and Glycine soja through population structure analysis. As a result, we are constructed the core collection of genetic diversity that reflects 99% of the entire collections, including 430 cultivated soybeans (Glycine max) and 386 wild soybeans (Glycine soja). The core collection developed in this study should be to provide useful materials for both soybean breeding programs and genome-wide association studies. Corresponding Author: Tel , mschoi73@korea.kr 199

244 OG-03 Jin-Kyung Kwon 1, Hea-Young Lee 1, Koeun Han 1, Na Young Ro 2, On-Sook Hur 2, Ho-Cheol Go 2 and Byoung-Cheorl Kang 1* 1 2 Pepper (Capsicum spp.) is one of the most popular vegetable that has medicinal and health benefits due to contain secondary metabolites such as capsaicinoid and carotenoid. In order to improve the power of the beneficial effects, many breeders and scientist were effort to figure out related genes or loci to control the diverse phytochemicals. Since the advance of genomics techniques, GWAS (Genome-wide association study) has become the most effective approach for identifying genetic variants associated to interested traits. For Capsicum GWAS, we have constructed a core collection as a natural population which can show the huge genetic diversity in reasonable size to control. From 2015, 19 quantitative traits and 4 kinds of secondary metabolites responsible for pungency were evaluated for 2 years. A genotyping-by-sequencing (GBS) approach was used to provide dense genome-wide marker (>9M SNPs) for a 355 pepper core collection. Population structure estimation (PCA and Kinship matrixes), and genome-wide association study (GWAS) based on the compressed mixed linear model were conducted using the R package of Genomic Association and Prediction Integrated Tool (GAPIT). As a result, average 26 SNPs distributed over 12 Capsicum chromosomes was identified that have association in each trait. For a validation, the associations were compared with well-known genes and the location of known QTLs which were surveyed from bi-parental population. It showed that, some QTL positions detected by GWAS were overlapped with previously detected QTLs in a parental population. These results will help to understand associations between phenotype and genotype and also will give more power to validate the candidate genes or quantitative trait loci. *Corresponding Author: Tel , sweettin@snu.ac.kr, bk54@snu.ac.kr OG-04 Sung-Taeg Kang 1*, Ju-Seok Lee 1, Jung-Kyung Moon 2, Soo-Kwon Park 2, Man-Soo Choi 2, Soon-Chun Jeong 3, Namshin Kim 4, Bo-Keun Ha Recent achievements in plant genomics by the development of high throughput sequencing technology have produced large amount of genomic data in soybean [Glycine max (Merr.) L.] and therefore, new gene identification strategies were employed to identify the quantitative/qualitative traits loci. A number of soybean cultivars for wide-range of purpose have been traditionally developed in Korea using broad range of germplasm including wild soybeans, since Korea is a center of origin in soybean. We developed Nest Associated Mapping (NAM) population to identify the useful genes and QTLs through fast and accurate high throughput procedure with Korean cultivars and germplasm. To develop the NAM population, the elite variety, Daepoong, was used as Hub parents and total 27 cultivars and lines were selected as parents in considering application-specific characteristics, such as physiological characteristics, polymorphism in DNA markers, cross combination, and desired characteristics for specific utilities. The selected parents and Hub parent were artificially crossed, and total about 3,000 lines of F 7 (or F 6) were developed using single seed descent. The genotyping for parents and NAM lines was conducted using the state-of-the-art Axiom 180K SoyaSNP array which is selected from more than four million high-quality SNPs identified from genome re-sequencing of 47 soybean accessions. The result showed that gene architecture can be disclosed with identification of the entire major and minor QTLs for flowering. The proposed NAM population could be very useful for further identification of agronomically important QTLs and genes. NAM population in soybean genomics could provide opportunity to discover the novel genes/qtls for various traits for sustainable crop breeding. *Corresponding Author: kangst@dankook.ac.kr 200

245 GSP 채소종자사업단 & GSP 원예종자사업단 2017년 7월 7일 ( 금 ), 09:00 ~ 10:40 대구엑스코 GSP채소종자사업단 & GSP원예종자사업잔

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247 OH-01 박용주 *, 이문호 하늘초고추 (erect type) 는강신미고추로주로아시아지역에서재배되는품종군에속한다. 재배면적은 80 만 ha~100 만 ha 이며아시아고추재배면적의약 40% 에달하는주요 segment 이다. 하늘초품종군에는단화방하늘초, 복화방하늘초, 탄두형 ( 볼 ) 하늘초등크게 3 개의 subgroup 로나뉘며이중단화방하늘초와복화방하늘초는 CGMS 를이용하여 F1 품종이보급되어재배되고있으나탄두형 (ball) 하늘초는아직일반종 ( 재래종 ) 으로재배되고있어서내병성이약하고수량성이낮다. 탄두형하늘초는주로중국남부지역및동남아지역에서주로재배되며이들지역은온도가높고강수량이많아바이러스및역병발생이심하여병저항성품종요구가높은실정이다. 탄두형하늘초의과는가공용 ( 염장 ) 및건과용의과형태로유통되어매운소스등으로이용되고있다. 본연구과제에서는탄두형 (ball) 일반종소재 ( 유전자원 ) 를이용하여최초로 CGMS F1 탄두 (ball) 형신품종육성연구를수행하였다. 수집된육종소재는 MAS 와재배시험을통하여원예적인형질과내병성 ( 역병, 바이러스 ) 평가를수행하였으며교배를통하여유전분석을실시하고이들재료들을활용하여다양한 B-line( 유지친 :maintainer) 계통과이를이용한웅성불임 (MS) A-line 계통, C-line( 임성회복친 :Rf) 계통을육성하였다. 육성된계통들은 F1 예비조합능력평가를통하여엘리트계통을선발하였으며선발된 F1 조합은현지연락시험을통해최종상품화하였다. * 주저자 : yjpark0226@hanmail.net OH-02 박은준 *, 양은영, 채수영, 문지혜, 박태성, 김옥례, 김대영, 이선이, 김상규 종자수출시장확대및수출액증대를위해서는각목표시장별우수한형질을가진자원의보유여부가매우중요하다. 우리나라에서고추육종에활용되어온유전자원은변이의폭이제한적이며, 수출시장이다원화되면서다양한형질의계통이필요한실정이다. 우수유전자원을고정시키고, 육종연한단축을위해서는배가반수체생산기술이요구된다. 본연구는실용적인고추소포자배양기술을확립하기위해모식물의생육시광도및생육기간이소포자배발생및발달에미치는영향과, 원예적특성분석을통해반수체 / 배가반수체의배수성을검증하였다. 그결과모식물은저광도에서단기간생육시소포자배발생효율이높았고정상자엽배의발생이가장많았다. 모식물생육환경을완전하게제어할수없는조건에서광도조절과생육기간을달리하는간단한방법을통해소포자배발생을크게증가시킬수있었다. 소포자유래의배를완전한식물체로생육후배수성검증을실시하였다. 총 208 개의식물체를분석한결과반수체는 89 개체, 배가반수체는 119 개체로재분화된식물체는반수체또는배가반수체이었다. 정확한분석을위해잎조직의염색체유동분석과식물체의원예적특성분석을실시하였다. 반수체와배가반수체의특성을조사한결과배가반수체는모식물과유사한경향이었고, 반수체의경우에는모든항목에서왜소한특성을보였으며, 특히잎과열매의크기는유의성있게작았다. 표현형에따른반수체와배가반수체분석은염색체유동분석방법과동일한결과를나타냈다. 반수체 / 배가반수체발생비율은밀양재래품종의경우 35.6%, LV2319 품종의경우 72.7% 로두배이상차이가났다. 소포자유래의배가반수체의효율이높은품종의경우에는직접적으로육종에이용할수있으나, 효율이낮은품종은콜히친처리후이용할수있다. 이와같은결과들은고추의소포자배양시스템을확립하는데기초자료가될것이며, 향후반수체육종프로그램에활용하는데유용하게이용될수있을것이다. * 주저자 : Tel , ejpark97@korea.kr 203

248 OH-03 최강준 나리 ( 백합 ) 는 Lilium 속의화훼식물로북반구에 130 여종이자생하고있는세계 4 대화훼작목중하나이다. 꽃의형태는 6 장의꽃잎으로이루어져있고종자와구근으로번식하는영년생의구근화훼식물이다. 대부분의원종이한국, 중국, 일본등동아시아를원산지로하고있으나상업적으로유통되는품종은네덜란드를중심으로개발되어세계적으로유통되고있다. 과거백합의세계적인시장은유럽, 미국, 일본이주요시장이었으나최근중국, 베트남에서화훼소비가급격히증가하고있어아시아에서의백합화훼시장이확대되고있다. 오리엔탈나리는꽃의크기가가장크고화려하며꽃의향기와절화수명이길어화훼시장에서가장많이유통되고있다. 이러한흐름에맞추어강원도농업기술원에서는 2001 년부터동아시아시장을목표로 Oriental hybrid 품종개발에집중하여추진하고있으며 2013 년부터는수입되는백합품종대체와수출확대를위해골든씨드프로젝트에참여하고있으며특히아시아시장공략을위한오리엔탈품종개발을위해프로젝트를수행하고있다. 강원도농업기술원에서는아시아시장공략을위한백합품종개발을위해 4 가지전략을갖고품종육성을추진하고있다. 첫번째는동아시아의기후에적합한품종개발을목표로추진하고있다. 기존품종이해양성기후의네덜란드에서개발되어유럽의기후에는적합하지만동아시아에서는기후적인차이로인해품질저하문제가발생하고있어동아시아기후에적합한품종개발을목표로품종육성을추진하고있다. 두번째는유럽인과아시아인의미적감각이다른점에착안을두어아시아인의미학적관점으로화색과화형의품종개발을추진하고있다. 세번째는품종개발기간을단축하여신속한품종개발시스템을구축하여시장의트렌드변화에빨리적응할수있도록하였다. 마지막으로네덜란드는적절한환경제어가되는온실을이용상자재배로절화생산이이루어지고있지만일본을비롯한아시아는대부분토경재배로생산되고있다. 아시아지역의토경재배에보다적응할수있고그러한환경에서도일정한품질을유지하는품종개발을추진하고있다. 현재 160 여계통과 7 품종이개발되었으며아시아시장현지적응을위해중국, 베트남, 몽골등아시아지역에서의개발품종의특성을검토하고있으며아시아시장구근수출을위한수출루트를개발하고있다. 주저자 : Tel , biockj87@korea.kr OH-04 박종인, 김회택, 노일섭 * 최근종자생산에있어서교잡, 선발등을이용한전통육종뿐만아니라분자육종기술의중요성이급격히커지고있는실정이다. MAS (Marker-assisted selection) 기술은목표형질의유전자를기반으로개체를선발함으로서전통육종의단점을보완하여우량개체선발효율을극대화시킴과동시에육종연한과투자비용을현저히감소시키므로종자산업계에서는우위경쟁력확보를위해서는필수적인기술로인식되고있다. 그러나현재국내종묘회사들중마커를자체적으로개발하여활용할수있는회사는극히일부회사를제외하고는없는실정이다. 또한육종가들은분자마커에대한필요성은인식하고있으나국내에서적용가능한분자마커들의종류, 선발효율성, 특성등이객관적으로파악되고정리되지않아그활용은극히미미한실정이다. 따라서, 국내원예작물의 MAS 기술지원을가속화하기위해서는종묘회사들에서필요로하는형질별요구도를명확히분석한후, 빠른시간내에종묘회사들에서요구하는형질별분자마커서비스가이루어져야한다. 그러기위해서는신규로개발이필요한분자마커개발연구와더불어전세계연구자들이이미개발하여발표한다수의원예작물분자마커들의정보들을수집하여실용성을검토한후실용성이확인된분자마커들은즉시종묘회사들에분자마커서비스가이루어져야한다. 본발표에서는수집된정보들을이용한분자마커개발및새롭게개발되고있는양배추의뿌리혹병 (CR), 검은썩음병 (BR), EP1 system 을이용한자가불화합성 S-genotype 구분용 chip 개발, 양파의순도검정용마커개발, 토마토의 MABC (Marker-assisted backcrossing) 용 chip 개발등의현재개발하고있는연구내용들에대하여소개하고, 본연구실에서수행하고있는분자마커서비스현황에대하여발표하고자한다. * 교신저자 : T , nis@sunchon.ac.kr 204

249 2017 년한국육종학회학회상시상

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251 2017 년한국육종학회학회상시상 - 수상자최봉호 ( 전충남대학교교수 ) - 선정사유국내재래종찰옥수수를다수수집하고적극적으로개량하여대한민국찰옥수수품질육종의지평을연찰옥수수 연농1호 ( 일명대학찰 ) 을육성 보급하고, 교단에서는작물육종의중요성일깨우고젊은육종가를배출하는등농가소득증대와인재양성에크게공헌 - 수상자 1 이정동 ( 경북대학교농업생명과학대학 ) - 논문제목 Reaction of Soybean Cyst Nematode Resistant Plant Introductions to Root-Knot and Reniform Nematodes (Plant Breeding and Biotechnology 2015, 3: ) - 수상자 2 이종열 ( 국립농업과학원농업생명자원부생물소재공학과 ) - 논문제목국내밀품종의오메가5-글리아딘 (Gli-B1) 유전자좌분석 ( 한국육종학회지, 2016, 48: ) 국내밀품종의글리아딘및 Spa 유전자평가 ( 한국육종학회지, 2016, 48: ) - 수상자 류수노 ( 한국방송통신대학교 ) - 품종명 슈퍼자미 [ 벼품종품종보호제4151호 ( )] - 수상자 이준설 ( 국립식량과학원바이오에너지작물연구소 ) - 품종명 풍원미 (Pungwonmi) 품종등록번호 : 6428 ( 고구마 (Ipomoea batatas (L.) Lam.)) - 수상자 (1) 박동수 ( 국립식량과학원남부작물부 ) - 논문제목 기능성흑찰거대배아미조생종벼품종 눈큰흑찰 ( 한국육종학회지, 2015, 47(1): 68-74) - 수상자 (2) 최홍규 ( 동아대학교생명자원과학대학 ) - 논문제목 Soybean [Glycine max (L.) Merrill]: Importance as A Crop and Pedigree Reconstruction of Korean Varieties (Plant Breeding and Biotechnology 2015, 3(3), ) - 수상자 (1) 정지웅 ( 국립식량과학원답작과 ) - 수상자 (2) 노일섭 ( 순천대학교생명산업과학대학 ) - 29 대학회장조용구 ( 충북대학교 ) 207

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253 색인

254

255 Name Page Name Page Name Page A-Ra Cho 105 Abinaya Manivannan 71, 77, 79 Adnan Younis 90 Ah-Rim Lee 111 Ana Cecilia Aliaga Fandino 37 Anh Quynh Le 103 Anil Kumar N.C 91 Anil Kumar Nalini Chandran 76, 115 Areum Lee 116 Ayoung Jung 166 Beom Seok Choi 66 Beom-Young Son 97 Beum-Chang Kang 26 Bing Yang 4 Bo-Keun Ha 53, 54, 55, 154, 156, 200 Bo-Kyeong Kim 61, 62, 112, 113, 114, 115 Bo-kyeong Kim 156, 157 Bong-Sik Yu 181 Byong Gon Choi 81 Byoung-Cheorl Kang 26, 71, 72, 104, 109, 166, 200 Byoung-Rourl Choi 192 Byung-Cheorl Kang 100 Byung-Chun In 23 Byung-Moo Lee 51 Chaeyoung Lee 50, 51, 135 Chan Seop Ko 128 Chan-Ho Kang 152 Chan-sung Oh 47 Chang Suk Huh 103 Chang-Ho Eun 93 Chang-Ho Kim 119 Chang-Hyu Bae 154 Chang-Sik Oh 101 Changsoo Kim 110 Chanseok Shin 89 Cheol Seong Jang 74, 89 Cheol seong Jang 24 Cheol Won Lee 130 Cheol Woo Min 22 Choi Yu Hyeon 90 Chon-Sik Kang 112, 113, 114, 115, 156, 157 Chong Ae Lee 105 Choon-Song Kim 61, 62 Chulsoo Park 112, 113, 114, 115 Chun-Song Kim 148 Chungryul Jung 96 Da-Eun Im 111 Da-Hye Kim 133 Da-Yea Park 88 Da-Young Baek 169 Dae Hui Chung 96 Dae Yeon Kim 67, 127, 128, 129 Dae-Hwa Yang 93 Dae-Jin Yun 37, 65 Dae-Woo Lee 23 Daeil Kim 117, 118, 119, 120 Daewoong Lee 109, 110, 111 Dal-A Yu 42, 113, 117 Daniel Hailegiorgis 105 David Yeh 14 Deok-Young Song 173 Do Yeon Kwak 86, 159 Do-Hon Kim 59 Do-Soon Kim 95, 164 Do-Sun Kim 77, 79 Do-Yeon Kwak 173 Doh-Hoon Kim 189 DoilChoi 101 Dong ho Lee 96 Dong Hyun Kim 190 Dong-Gun Kim 154, 156, 164 Dong-Hee Kim 199 Dong-Hui Kang 147 Dong-Min Kim 42, 65 Dong-Seon Kim 36 Dong-Soo Park 82, 131, 182 Dongho Lee 88 Dongjin Shin 82, 131 Dong Min Kim 60 Doo-Sang Park 92 Dool Yi Kim 91 Doori Park 89 Doyeon Hwang 109 DoYeon Kwak 169, 189 Doyeon Kwak 172 Duk-Ju Hwang 104 Enrico Martinoia 38 Eokkeun Ahn 85 Erin Bell 178 Eun Jeong Lee 129 Eun Ju Lee 184 Eun Jung Suh 91 Eun Su Lee 77 Eun Young Oh 86, 159 Eun-Gyeong Kim 92 Eun-Jo Shim 65 Eun-Jo Sim 60 Eun-Ju Byeon 81 Eun-Su Lee 79 Eun-Young Oh 173 Eun-Young Yang 79 Eungyeong Lee 160 EunYoung Oh 169, 189 Fahad Ramzan 90 Fenglin Deng 38 Franz M. Nogoy 116, 117 Franz Marielle Nogoy 42, 195 G.D. Prahalada 21 Ga-Hee Kim 171 Gang-Seob Lee 81, 91, 98, 99 Geung-Joo Lee

256 Name Page Name Page Name Page Gi Jun Choi 178 Gi-An Lee 169, 170, 171 Gi-Heung Hong 112, 113, 114, 115 Giha Song 88 Gihwan Yi 59 Giwon Cho 92 Goh Choe 37 Gregory B. Tilton 178 Gun-Mi Lee 62 Gwang Hun Park 96 Gynheung An 22, 91, 115 Gyoungju Nah 104 Gyu Tae Park 52, 53, 69, 142 Gyu-Chan Shim 65 Gyu-Myung Lee 101 Ha Thi Lieu 70 Hacheol Hong 76 Hae Ryong Cho 183 Haeun Jung 142 Hak Soo Seo 52, 53, 66, 67, 69, 142 Hak-Ki Shin 181 Han Sol Kang 174 Han-Yong Um 47 Hana Jeong 93 Hans Vasquez-Gross 43 Hawk-Bin Kwon 135 Hayoung Song 108 Hea-Young Lee 200 Hee Chung Ji 178 Hee Ju Yoo 100, 101 Hee-Jong Koh 72, 73, 123 Hee-Jong Woo 181 Hee-Jung Sim 26 Heebak Choi 22, 122 Heng Wang 111 Heung-ryul Lee 104 Ho Jun Joh 36, 41, 77, 80, 100 Ho-Cheol Go 200 Hong Gil Lee 84 Hong Jib Choi 103 Hong-Il Choi 67, 74, 157, 161 Hong-il Choi 39 Hong-Kyu Choi 50, 51, 135 Hong-Sik Won 137 Hongryul Ahn 135 Hongseock Lee 160 Hwan-Hee Bae 97 Hyangmi Park 76 Hye Jeong Kim 37, 40, 64, 65 Hye Ryun An 134 Hye Seon Kim 104 Hye Sun Cho 116 Hye-Jung Lee 42, 114 Hye-min Lim 98, 99 Hye-Won Chang 123 Hyejin Yu 95 Hyekyung Son 72 Hyenso Ji 160 Hyeon-Jin Sun 93 Hyeon-so Ji 98, 99 Hyeondae Han 117, 118, 119, 120 Hyeong Seok Kim 149 Hyeonso Ji 25, 81, 85 Hyeran Kim 26, 27 Hyo-Bong Jeong 166 Hyo-Ju Lee 190 Hyo-min Ahn 26 Hyo-suk Kim 149 Hyo-Yeon Lee 93 Hyo-Yong Kim 172 Hyochul Kim 51 Hyoung Tae Kim 73, 90 Hyoung-Ho Park 156, 157 Hyun A Jang 107 Hyun Hee Kim 35, 136 Hyun Oh Lee 100 Hyun Suk Cho 37, 40, 64, 65 Hyun Uk Kim 50, 136, 142, 180 Hyun Ungjo 76 Hyun-Ju Kang 85 Hyun-Oh Lee 36 Hyun-Seok Chae 156, 157 Hyun-Seung Park 41 Hyun-Sook Lee 42, 103, 106 Hyun-Soon Kim 52 Hyun-Su Park 52, 61, 62, 148 Hyun-Suk Cho 181 Hyung Seong Lee 142 Hyung-Keun Ku 88, 96 Ick-Hyun Jo 150 Ik Young Choi 81 Ik-young Choi 89 Il-Pyung Ahn 104, 123, 199 Il-Sheob Shin 61 Ill Sup Nou 195 In Hye Lee 195 In-Seok Oh 173 In-Sok Lee 152 Inchan Choi 25, 85, 160 Inhwa Yeam 101 InhwaYeam 101 Inseo Kim 77, 80, 100 Inseok Oh 172 Inuk Jung 135 Inyoung Kim 136 Ja Kyong Ko 24 Jae Hun Kim 149 Jae Kwang Kim 96 Jae Seong Kim 37, 40, 64, 65 Jae Uk Lee 190 Jae Yong Han 37, 40, 64, 65 Jae Yoon Kim 51, 119 Jae-A Jung 181 Jae-Beom Chun 52 Jae-Han Son 112, 113, 114, 115 Jae-Hee Lee 135 Jae-Ki Song 112, 113, 114, 115 Jae-Kwon Ko 52 Jae-Ryoung Park 189 Jae-Young Song 113, 116, 117 Jae-Young Yun 26 Jaean Chun 61 Jaehyeon Jeon 77, 80 Jagadeesh Sundaramoorthy 52, 53, 69, 142 Jahee Ryu 26 Jaihyunk Ryu 154, 156, 164, 174 Jang-Gyu Choi 137 Jang-Uk Kim 150 Je Hyeong Jung 24 Je Min Lee 73, 100,

257 Name Page Name Page Name Page Je Wook Woo 26 Jee Young Park 39, 41, 100 Jeeyeon Ko 172 Jegeun Cho 176 Jelli Venkatesh 26 Jeom-Sig Lee 21 Jeong Dong Lee 103 Jeong Eun Kim 100 Jeong Hee Lee 86 Jeong Suk Bae 103 Jeong-Dong Lee 52, 53, 69, 142 Jeong-Gwan Ham 105 Jeong-Hee Lee 80 Jeong-Il Kim 40, 64 Jeong-Kwon Nam 61, 62, 148 Jeong-Suk Bae 112, 113, 114, 115 Jeong-Tak An 71 JeongHo Baek 25, 160 Jeongho Baek 85 Ji Yun Lee 103 Ji-Eun Hong 150 Ji-Hong Cho 137 Ji-Hye Hwang 123 Ji-Hye Lee 192 Ji-Min Yoo 199 Ji-Ung Jeong 52 Ji-Yoon Lee 82, 131, 182 Ji-Young Lee 37 Ji-Young Son 92 Jian Feng Ma 38 JiMyoung Choi 169, 189 Jin A Kim 59 Jin Baek Kim 128 Jin Hoon Lee 151 Jin seok Moon 73 Jin Seok Yoon 129 Jin Sol Park 37, 40, 64, 65 Jin Sun Kim 134, 191 Jin-A Kim 147 Jin-Baek Kim 74, 154, 156, 157, 161, 164, 174 Jin-Beak Kim 67 Jin-Hee Kim 106 Jin-Hee Lim 23 Jin-Ho Kang 26 Jin-Hyun Kim 50, 51, 135 Jin-Kee Jung 60 Jin-Kyeong Choi 112, 113, 114, 115 Jin-Kyung Kwon 26, 200 Jin-Seok Lee 97 Jin-Soo Kim 26 JinHee Kim 77 Jinhee Kim 79 Jinhuan Wei 22 Jinkwan Jo 104 Jinmi Yoon 22 Jinsu Gil 96 Jinwoo Lee 72 Jisu Choi 92 Jiyeon Kim 117, 118, 119, 120 Joie Ramos 21 Jong Bae Kim 176 Jong Hee Kim 190 Jong Hyang Bae 83, 108 Jong Nae Hyun 128 Jong Pil Hong 77 Jong Tae Song 25, 52, 53, 69, 142 Jong-Chan Park 91 Jong-Chul Park 112, 113, 114, 115, 156, 157 Jong-Hee Lee 82, 131, 182 Jong-Ho Park 156, 157 Jong-Il Chung 147 Jong-Min Jeong 61, 68, 148 Jong-Seong Jeon 23, 76 Jong-Taek Park 181 Jong-Yeol Lee 131, 132, 133, 134, 191 Jongcheol Ko 172 Joo Yeol Kim 59 Joo Yong Kim 67 Joo-Seok Park 50, 51, 135 Joohyun Lee 92 Joon Ki Hong 91 Joon ki Kim 117 Joon-Woo Ahn 67, 74, 161, 164 Joong Hyoun Chin 47 Joonki Kim 114 Joonyup Kim 92 Jorge Dubcovsky 43 José M Jiménez-Gómez 83 Joung Sug Kim 60 Joung-Ho Lee 71 Ju Kyong Lee 41, 56, 57, 58, 81, 145 Ju Seok Lee 55 Ju-Kon Kim 16, 91, 96 Ju-Seok Lee 200 Ju-Seong Im 137 Juhun Lee 109 Juhyun Im 53, 54, 55 Jun Oh 85 Jun Soo Kwak 66 Jun Young Choi 184 Jun-Cheol Moon 51 Jun-Hoi Kim 105, 106 Jun-Hyeon Cho 82, 131 Jun-Hyun Cho 182 Jundae Lee 80, 151 June Oh 160 June-Seob Eom 23 Jung Ae Park 133 Jung In Kim 86, 159 Jung Min Kim 154, 156 Jung-Hee Jang 192 Jung-Hoon Choi 47 Jung-Il Cho 98, 99 Jung-In Kim 173 Jung-Kyung Moon 55, 123, 199, 200 Jung-Min Kim 164 Jung-Ro Lee 169, 170, 171 Jung-Rye Nam 124, 125, 199 Jung-Tae Kim 97 Jung-Woo Lee 150 JungIn Kim 169, 189 Jungmin Park 160 Junki Lee 36, 39, 41 Junkyoung Choe 23 Juyeon Jung 36 Juyoung Kim 84 Kabita Sarker 93, 94 Kang-Hyun Lee 98, 99 Kazuhiro Nemoto 47 Kee Hoon Sohn 10 Kee-Choon Park 150 Kenichi Matsushima 47 Keumsun Kim 117, 118, 119, 120 Keunpyo Lee 23 Ki Hong Jung 70 Ki Jin Park 91 Ki Won Oh 86, 159 Ki-Byung Lim

258 Name Page Name Page Name Page Ki-Hong Hong 156, 157 Ki-Hong Jung 76, 91, 115, 172 Ki-Kwon Lee 152 Ki-Won Lee 178 Ki-Won Oh 80, 173 Ki-Yong Kim 178 Kidong Hwang 117, 118, 119, 120 Kihwan Song 77, 80 Kitae Song 51 KiWon Oh 169, 189 Kiyoon Kang 21 Kiyoung Kim 172 Koeun Han 71, 72, 104, 109, 166, 200 Kong-Sik Shin 181 Kristin Bilyeu 8 Kshirod K. Jena 21 Kukhyun Jung 76 Kwang-Soo Cho 137 Kwansik Woo 172 Kwon Kyoo Kang 113, 190 Kwon Seok Jeon 96 Kwon-Kyoo Kang 42, 116, 117, 190, 195 Kyeong-Ho Kang 68 Kyeong-Hoon Kim 112, 113, 114, 115 Kyeong-Ryeol Lee 180 Kyeong-Seong Cheon 80, 81 Kyo-Sang Lee 107 Kyong Cheul Park 41, 81 Kyong Mi Jun 60 Kyong Sil Lee 104 Kyong-Cheul Park 57, 58 Kyong-Ho Kim 112, 113, 114, 115, 156, 157 Kyong-Hwan Bang 150 Kyoung Bok Lee 96 Kyounghee Lee 83 Kyoungwon Cho 132 Kyu Ho Lee 67 Kyu Jin Sa 56, 57, 58 Kyu-Chan Shim 103, 106 Kyu-Hwan Choi 156, 157 Kyu-Won Kim 123, 124, 125, 126, 199 Kyung Eun Lee 176 Kyung Hun Kim 128 Kyung Jun Lee 169, 170, 171 Kyung-Hee Kim 51 Kyung-Ho Kang 52 Kyung-Ho Ma 169, 170, 171 Kyung-Hwan Kim 25, 85, 91, 160 Kyung-Min Kim 82, 105, 106 Lan Cho 53 Le Thi Dung 50 Lee Dasom 38, 70 Lee Jae Woo 89 Li Liu 26 Lincoln Ong 28 Luis Alejandro de Haro 43 Luong Ngoc Ha 106 Man-Kee Baek 61, 62, 148 Man-Soo Choi 199, 200 ManJo Kim 96 Marjohn Nino 42, 116, 117 MBA 29 Md. Amdadul Huq 195 Md. Atikur Rahman 178 Me-Sun Kim 42, 113, 116 Megan Sweeney 114 Meong Kyu Ou 128 Mi Chung Suh 84 Mi Jeong Jeong 59 Mi Ran Ko 96 Mi-Seon Kim 181 Mi-Young Park 93 Mia Park 178 Michael Kovach 114 Mid-Eum Park 180 Mijeong Kim 92 Min Jeong Hong 67, 128, 161 Min Jeoug Hong 129 Min Jung Kim 98 Min Kang 98, 99 Min Kyoung You 96, 112, 121 Min Kyung Choi 26 Min-Gi Lee 117, 118, 119, 120 Min-Gyun Jeong 50, 51, 135 Min-Kwon 137 Min-Kyoung You 88 Min-Kyu Lee 164 Min-kyu Lee 154, 156 Min-Sung Kang 108, 195 Min-Young Kang 26, 109, 166 Minjung Yook 95 Minkyoung Kim 77 Mockhee Lee 118, 119 Moo-Chan Kang 166 MS.Eng. 29 Muhammad Irfan Siddique 71, 72 Muhammad Qudrat Ullah Farooqi 57 Mummadi Reddy Ramya 134 Munetaka Hosokawa 26 Murukarthick Jayakodi 39 Myeong Hyeon Nam 151 Myeongen Choe 172 Myoung Hee Lee 86 Myoung-Hee Lee 80 Myoung-Jae Shin 169, 170, 171 MyoungHee Lee 169, 189 Myung Hee Lee 159 Myung Ok Byun 104 Myung Sik Kim 86 Myung-Hee Lee 173 Myung-Ho Lim 181 Myung-Kyu Oh 82, 131, 182 Na Young Ro 200 Na-Hyun Shin 47 Nam-Chon Paek 21, 88 Nam-Hoon Kim 39, 41 Nam-In Hyung 81 Namshin Kim 200 Naoki Yamaji 38 Neha Samir Roy 41 Nomar Espinosa Waminal 35, 39, 136 Norvie L. Manigbas 59 Nyunhee Kim 160 Ok-Kyu Han 156,

259 Name Page Name Page Name Page Ok-Sun Park 83 On-Sook Hur 200 Ouk-Kyu Han 112, 113, 114, 115 Paulina Calderón Flores 127, 128, 176 Phitaktansakul Rungnapa 199 Pil Joon Seo 83, 84, 190 Ping Jin 22 Preethi M Purushotham 104 Pue Hee Park 134 Qiang He 122, 126, 199 Rahmatullah Jan 105 Rahul Vasudeo Ramekar 41, 57, 58, 81 Ramesha H. Jayaramaiah 22 Ravi Gupta 22 Remnyl Joyce Pellerin 35, 136 Rupesh Tayade 38, 70 Russell Reinke 68 Ryuli Gang 54 Sae Hyun Lee 36, 77, 80 Saes-Beul Lee 82, 131 Saima Akhter 70 Sais-Beul Lee 182 Sampathkumar Palaniswamy 52 Sandra Goritschnig 26 Sang Gon Kim 97 Sang Gu Kang 176 Sang Hoon Kim 67, 74, 161, 174 Sang Hun Kim 53, 54 Sang Ryeol Park 104 Sang Woo Park 66 Sang-Bok Lee 60 Sang-Choon Lee 36, 39, 41, 77, 80, 100 Sang-Gyu Kim 26 Sang-Ho Chu 199 Sang-Hoon Lee 178 Sang-Ik Han 82, 131, 182 Sang-Keun Oh 107 Sang-Kyu Lee 23 Sang-Kyu Park 133 Sang-Nag Ahn 42, 103, 106 Sang-Tae Kim 26 Sang-Won Lee 91 Sang-Won Park 199 Sang-Woo Choi 147 Sanghun Kim 55 Sangsu Bae 190 Sanwen Huang 6 Se Jong Kim 103 Sea-Hee Han 171 Sebastian Soyk 83, 195 Sena Choi 79 Seok-Hyun Choi 23 Seok-Jun Moon 104 Seokbo Song 172 Seokhyung Lee 69, 142 Seon-Hwa Bae 80 Seon-Ju Shin 135 Seon-Kyeong Song 113 Seong-Bum Baek 97 Seong-Gyu Jang 111 Seong-Kon Lee 181 Seong-Woo Cho 93, 94 Seongbin Hwang 142 Seul-Gi Park 61, 62 Seul-Ki Lim 60 Seula Choi 71 Seung Bum Lee 91 Seung Cheol Oh 53 Seung-Eun Park 65 Seung-In Yi 60, 65 Sewon Oh 117, 118, 119, 120 Shahina Akter 190 Sherry Lou Hechanova 21 Shi Jun Ma 56, 57, 145 Shin-Chul Bae 104 Shin-Jae Kang 41 Shin-Sook Park 65 Si-Yong Kang 53, 54, 67, 74, 154, 156, 157, 161, 164, 174 So-Young Kim 98, 99 Somyung Lee 73 Song Joong Yun 105 Song Lim Kim 25, 85, 160 Songhwa Chae 60 Soo In Lee 59 Soo Jeong Kwon 93, 94 Soo-Cheul Yoo 47 Soo-Chul Park 98, 99 Soo-Hyun Lim 95, 164 Soo-Kwon Park 200 Soon Ju Park 83, 108, 195 Soon Ki Park 38, 69, 70 Soon-Chun Jeong 55, 123, 200 Soon-Jae Kwon 74, 154, 156, 161, 164 Soon-Wook Kwon 61, 111 Sopheap Yun 105, 106 Sota Koeda 26 Sothea Ouk 42, 113, 117 Sovetgul Asekova 159, 173 Stephen Pearce 43 Su Jang 72 Su Young Lee 183 Su-Jin Cho 134 Su-Jin Choi 191 Su-Jin Lee 147 Su-Min Jo 82, 131, 182 Suji Ahn 117, 118, 119, 120 Suji Bae 26 Suk Bok Bae 159 Suk Bok Pae 86 Suk-Bok Bae 173 Suk-Bok Pae 173 Suk-Man Kim 68 SukBok Pae 169, 189 Sun Gang Choi 81 Sun Ha Kim 42, 103, 106 Sun Kim 135 Sun Tae Kim 22 Sun-A Park 115 Sun-geum Jeong 184 Sun-Goo Hwang 74 Sun-Hee Woo 93, 94 Sun-Hwa Ha 88, 96, 112, 121, 122 Sun-Hyung Lim 88, 96, 131, 132, 133, 134,

260 Name Page Name Page Name Page Sun-Mi Lee 24 Sung Aeong Oh 38, 69, 70 Sung Min Han 74, 157 Sung Ok Park 133 Sung Up Kim 159 Sung-Chur Sim 23 Sung-Hwan Cho 21 Sung-Hwan Jo 53 Sung-Jin Han 147 Sung-Min Youn 35, 136 Sung-Ruyl Kim 115 Sung-Ryul Kim 21 Sung-Taeg Kang 200 Sung-Up Kim 173 Sunggil Kim 36 Sungtaeg Kang 55 SungUp Kim 169, 189 Sungyul Chang 149 Sunheum Cho 120 Sunyoung Moon 26 Susan B Altenbach 131 Susan B. Altenbach 12 Susan McCouch 114 Swapan Kumar Roy 93, 94 Tae Sung Park 77 Tae Young Hwang 178 Tae-Heon Kim 82, 131, 182 Tae-Ho Kim 80, 81, 86 Tae-Hwa Song 156, 157 Tae-Hwan Jun 79, 109, 110 Tae-Il Park 112, 113, 114, 115, 156, 157 Tae-Jin Won 192 Tae-Jin Yang 35, 36, 39, 41, 77, 80, 98, 100, 136 Taegyu Kim 92 Taehwan Jun 111 Taejun Kim 72 Taek-Ryoun Kwon 25, 160 Taewook Jeong 172 Taewook Kim 89 Taiyong Quan 22 Tak-Ki Hong 56 Thi Hai Yen Nong 53 Tien Dung Nguyen 38, 69, 70 Tomotaro Nishikawa 47 Tyson Howell 43 Un Sang Yeo 86, 159 Un-Sang Yeo 173 Ung-Han Yoon 80, 81 UnSang Yeo 169, 189 Van Binh Nguyen 98 Vo Ngoc Linh Giang 98 Wan Woo Yeom 37, 40, 64, 65 Wei Tong 122 Wei-Guo Zhao 199 Weiguo Zhao 126 Win Htet Oo 125, 199 Won Joo Shin 66 Won Suk Jang 151 Won Yong Jung 116 Won-Hee La 123 Won-Kyung Lee 77, 80 Won-Tae Yang 189 Won-Yong Song 38 Woo Joo Jung 130 Woo Sub Kim 66 Woo-Jae Kim 52, 61 Woo-Jong Hong 76 Woojong Jang 39, 41 Woon Ji Kim 53, 54, 55 Woon-Chul Shin 62 Woon-chul Shin 148 Wricha Tyagi 114 Xiao-Han Wang 170 Xiao-Xuan Du 105, 106 Xin Lu 106 Yang Qin 181 Yang-Hee Cho 169, 170, 171 Yang-Kil Kim 112, 113, 114, 115, 156, 157 Yang-Seok Lee 22 YangJae Kang 111 Ye Rim Lee 190 Ye Rin Lee 151 Ye Sol Jeong 88, 96 Ye-Ji Lee 81 Yeeun Jang 39 Yeo Jin Jung 190 Yeo Jin Lee 121 Yeo-Ok, Park 92 Yeon-Hee Lee 91 Yeon-Ho Park 164 Yeon-Ki Kim 60 Yeon-Ok Choi 36 Yeong Deuk Jo 26, 74, 161, 164, 174 Yi Lee 96 Yo-Han Yoo 91 Yogesh Gupta 37 Yong Bog Kim 81 Yong Chul Kim 86 Yong Gu Cho 195 Yong Weon Seo 67, 119, 127, 128, 129, 130, 176 Yong-Chul Kim 173 Yong-Gu Cho 42, 113, 114, 116, 117, 190 Yong-Jin Park 122, 123, 124, 125, 126, 199 Yong-Sam Kwon 59 Yong-Sham Kwon 189 Yong-Soo Choi 199 Yong-Wook Ban 89 Yongjae Won 76 YongSuk Chung 110 Yoo-Hyun Cho 199 Yoon-Kyeong Kim 61, 117, 118 Yoon-Sup So 169, 171 Yoonjung Lee

261 Name Page Name Page Name Page Yoonkang Hur 108 Yoonkook Park 160 Yoram Choi 50, 51, 135 You-Chun Song 61, 82, 131, 182 You-Ran Jang 131, 132, 133, 134, 191 youjin Shin 26 Youn Jung Choi 183 Youn Sung Kim 41 Youn-Sung Kim 116, 184 Young Eun Jang 161 Young Joo Jung 96 Young Kwan Jo 90 Young Ran Lee 183 Young Soo Chung 37, 40, 64, 65 Young-Chan Cho 61, 62, 148 Young-Chang Kim 150 Young-Eun Park 137 Young-Han Lee 92 Young-Jin Oh 156, 157 Young-Jun Park 47 Young-Keun Cheong 112, 113, 114, 115, 156, 157 Young-Mi Kim 131 Young-Mi Yoon 112, 113, 114, 115 Young-Sang Lee 199 Youngchul Chung 149 Younghoon Park 23, 92 Youngjun Mo 43 Youngmi Choi 92 Youngsook Lee 38 Yu Jin Jung 190 Yu-Jin Jung 42, 190, 195 Yul-Kyun Ahn 77, 79, 111 Yulho Kim 76 Yun Im Kang 183 Yun Sun Lee 98, 100 Yun-A Jeon 42, 106 Yun-Shil Gho 91, 115, 172 Yunfei Wu 22 Yunmi Park 96 Yurry Um 96 Zachary B Lippman 83, 195 Zhanguo Xin 93, 94 Zhi-Fang Zuo 93 Zhuo Jin 73 ᄀ 강경원 144 강경호 145, 152, 174, 179 강범규 56, 63, 82 강상호 192 강시용 138, 139, 144 강신구 146 강위금 62, 144, 146 강주원 39 강준원 167, 168 강지선 140 강천식 146, 165, 177 강택규 49 강항구 30 강홍규 94 고건희 141 고상욱 151 고재권 145, 152, 153, 179 고종민 56, 63, 82 고종철 145, 152, 155 고지연 155 고호철 107 곽도연 56, 63, 82, 155 구자환 159 권순욱 99, 138, 139, 179 권순종 159 권오덕 58 권오창 182 권용락 90 권유리 84, 160, 175 권택윤 161 금보라 35 김경민 84, 160, 175 김경호 165, 177 김경환 161, 162, 163 김경훈 84, 160, 175 김기영 145, 152 김기용 168 김남훈 28, 191, 192 김년희 161, 162, 163 김대식 62 김대영 186, 203 김도선 71, 185 김동민 140 김동용 99 김명권 31 김명기 75, 153, 174 김미선 68, 78, 79 김미진 127 김민주 48, 78, 151, 184 김백준 144 김병주 153, 162, 174 김보경 40, 62, 145, 152, 153, 177, 179, 180 김상곤 158, 187 김상규 186, 203 김상열 58 김선하 110 김성국 158 김성업 155 김성철 175 김세종 126 김세현 102 김송림 161, 162, 163 김양길 165, 177 김영미 90 김영진 149, 168 김옥례 186, 203 김용호 54 김우재 62, 145, 152, 180 김웅범 27 김원호 149, 168 김은경 175 김은옥 141 김재덕 49 김재명 166 김재희 102 김정곤 143 김정인 155 김정주 62, 144, 145, 146, 152 김정태 158, 187 김정호 185 김종보 121 김지은 27, 146 김지혜 149, 168 김진욱 29 김진희 71, 185 김춘송 40,

262 Name Page Name Page Name Page 김태영 29 김태형 139 김판기 168 김학신 137, 138 김현순 127, 145, 152, 180 김현영 63 김현철 167, 168 김현태 56, 63, 82, 140, 141 김형태 64 김혜식 191 김호방 48 김홍식 56, 63 김회택 204 ᄂ 남궁민 74, 143, 188 남민희 58, 158 남상식 166 남정권 40, 62, 145, 152, 153 노나영 107 노일섭 204 ᄃ 도재왕 87 ᄅ 류수노 179 류시환 74, 143, 188 ᄆ 문기범 127 문중경 163 문지혜 186, 203 ᄇ 박기진 74, 143, 188 박노봉 58, 158 박동수 58, 158 박두상 175 박문웅 143 박미소 28, 192 박미자 143 박민영 188 박병명 158 박부희 163 박상근 68 박석만 48, 78, 151, 184 박석진 87 박선경 121 박소연 99 박수형 188 박슬기 40 박승민 168 박여옥 175 박영기 102 박영준 140, 141 박용주 203 박용진 138, 139 박은영 87 박은준 186, 203 박재호 48, 78, 151, 184 박종열 74, 143, 188 박종인 204 박종철 165, 177 박종택 68, 78, 79 박종호 165, 177 박철수 49, 137, 138, 146 박태성 186, 203 박태일 165, 177 박현수 40, 62, 145, 152, 153 박형준 30 박형호 165 박효진 84 배민지 86, 182, 186 배상수 127 배석복 155 배은경 97 배정숙 126, 165, 177 배환희 158, 187 백만기 40, 62, 145, 152, 153 백성범 158, 187 백연경 49 백인열 56, 63 백정호 161, 162, 163 백형진 107 ᄉ 사미나단수브라야 127 서대하 162, 174 서영호 74, 143 서용원 90 서정필 75 서정현 56, 63, 82 선현진 94 성낙식 174 성병열 143 성열규 162 성정숙 107 손민식 140 손범영 158, 187 손영보 158 손지영 175 송석보 155 송연상 166 송원용 110 송유천 58, 158 송윤호 95 송재기 165, 177 송태화 165 신동진 84, 158, 160, 175 신명주 191 신상욱 56, 63 신운철 40, 62, 144, 145, 146, 152, 153 신학기 78, 79 심규찬 110 ᄋ 안경구 31 안광환 175 안동춘 86, 186 안상낙 39, 110 안승현 166 안억근 75, 153, 174 안영은 107 안율균 71, 185 안종웅 159 안혜림 90 양대화 94 양미희 29 양은영 186, 203 양정욱

263 Name Page Name Page Name Page 양창인 75, 146, 153, 174 양환래 121 여운상 58, 155 연영자 141 오기원 63, 82, 155 오동진 29 오명규 84, 158, 160, 175 오성환 58, 158 오세관 153 오영진 165 오은영 63, 155 오인석 63 오인여 158 오재은 27 오준 162 오태영 126 왕헝 99 용우식 74, 143, 188 우관식 155 원용재 75, 153, 174 유의수 28, 191, 192 유재흥 31 유주 141 윤건식 165, 177 윤미라 153 윤석원 74, 143, 188 윤성란 175 윤수현 48, 78, 151, 184 윤영미 146 윤재복 87 윤홍태 56, 63, 82 이건미 40 이긍주 127 이기원 48 이동훈 151 이명희 155 이문호 203 이미자 177 이민석 141 이민휘 140 이병원 56, 63, 82 이봉우 27 이상복 75, 153, 162, 174 이상협 187 이상희 121 이선이 186, 203 이솔지 167, 168 이솔화 140 이승욱 28, 192 이영길 140 이영복 145, 152 이영상 138, 139 이영한 175 이영훈 63, 82 이원도 138, 139 이위영 167, 168 이은경 161, 162, 163 이은수 71, 185 이은혜 79 이재훤 140, 141 이점호 75, 145, 152 이정대 141 이정동 87 이정수 188 이정희 27, 75, 153, 162, 174 이종훈 31 이종희 58, 158 이주영 54 이주희 107 이준대 35, 107 이준설 166 이지우 95 이지윤 58, 126, 158 이진석 158, 187 이진호 141 이현석 167, 168 이현숙 39, 110 이현오 28, 191 이형운 166 이홍석 161, 162, 163 이효신 97 이효연 94 임기병 64 임용표 188 임혜민 167, 168 ᄌ 장경환 167 장은하 74 장재기 75, 153 장지영 127 장하영 188 전영아 107 전윤아 39 전재범 145 정건호 158 정국현 75, 153, 174 정규미 35 정남희 163 정미남 166 정영근 146, 165, 177 정용모 86, 182, 186 정윤재 29 정응기 75, 153 정재아 68, 78, 79 정재혁 144 정종민 75, 145, 152, 153, 162, 179, 180 정종성 149 정지웅 145, 152, 179, 180 정지희 90 정찬식 63, 155 정태욱 155, 158 정하나 94 정희채 153 조성우 49, 137, 138, 146 조성환 27 조수현 177 조신환 185 조아르나 90 조영득 144 조영일 32 조영찬 40, 62, 145, 152, 153, 174 조유현 138, 139 조윤상 95 조준현 58, 158 조현숙 146 좌지방 94 지현소 161, 162, 163 지희정 48 진성범 48, 78 진영돈 86, 182, 186 ᄎ 채수영 186, 203 최강준 204 최경후 141 최근진 29 최만수 63, 82, 163 최명은

264 Name Page Name Page Name Page 최범순 28, 191, 192 최성태 175 최승국 71 최시림 86, 182, 186 최영임 97 최영훈 151 최용석 137, 138 최용현 137, 138 최유미 155 최유현 64 최인찬 161, 162, 163 최재근 74, 143, 188 최재성 143 최준경 27 최준근 74, 143, 188 최진경 177 최현모 97 최홍집 126 ᄒ 하건수 95 하운구 75, 153, 174 하태정 82 한상익 158, 175 한선경 166 한옥규 159, 165, 177 한원영 56, 63, 82 한희정 163 함태호 179 허온숙 107 허창석 126 현웅조 75, 153, 162, 174 현종내 84, 160, 175 홍광표 86, 182, 186 홍기흥 165 홍종필 71 홍지화 140, 141 홍하철 75 황병호 187 황엄지 166 황주천 86, 186 황태영 48,

265 인쇄 2017년 6월 27일 발행 2017년 7월 5일 발행자 사단법인한국육종학회 경기도수원시권선구수인로 126 국립식량과학원중부작물부내 인쇄처 교보문고 ( ) 경기도파주시광인사길 226 두성종이빌딩 4층