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理蓮臨類 V CNPH-93-10 行 93 年 1 1 93 年 12 31 參行理 94 年 2 21

濫不諸類料 (1) 歷年來 (70-92 年 ) 六更年來 (80-92 年 ) 療金蓮葉龍蓮蓮療 (2) 蓮葉 Scutellria barbata Don 臨 (3, 4) 蓮 D- 糖 (D-galactosamine) (Acetaminophen) 不 (5) 蓮 Chou (6) (7-12) 蓮 Baicalein Baicalin Wogonin 蓮理 Kimuya (13) Abe (14) (15-19) Baicalein Baicalin Wogonin apigenin luteolin 類列殺 (20) 茶 protocatechuic acid 類 (21) 蓮不療療臨見都量便 1976 年 (22) 量 3.5 % 49.8 % 46.7% (23-27) (28) (29) 臨流 (23-26,,28, 30-31) 臨流行綠臨流良離臨度 (304.2 K) 臨力 (1070.7 psi) 行别離不便率易離臨蓮 naringenin luteolin protocatechuic acid wogonin baicalein baicalin apigenin 量益理

1. 蓮粒量 105 量蓮量 12.1 % 量 (dry basis) 2. 臨 (SFE) 臨流 ISCO 臨流良量力 40Mpa 數 (Fig. 1) 量蓮 (cartridge) 度力行 HPLC 度 3. Naringenin Aldrich HPLC 度 HPLC JASCO UV/Vis (photodiode) 離 Inertsil 5C 18 ODS-2 4.6 mm 度 250 mm 度 45 流流 1 ml/min protocatechuic acid 流 0.1% H 3 PO 4 270 nm naringenin luteolin baicalin apigenin 流 0.1% H 3 PO 4 -CH 3 CN (80:20, v/v) 280 nm baicalein 流 0.1% H 3 PO 4 -CH 3 CN (75:25, v/v) 280 nm wogonin 流 0.1% H 3 PO 4 -CH 3 CN (70:30, v/v) 280 nm 論 1. HPLC 精量 0.5 mg/ml Naringenin 六 (intraday) 連六 (interday) 兩 HPLC 精 wogonin baicalein baicalin naringenin luteolin apigenin protocatechuic acid intraday (RSD) 3.41 2.92 3.71 2.18 3.06 2.87 3.64 wogonin baicalein baicalin naringenin luteolin apigenin protocatechuic acid interday (RSD) 3.83 3.04 4.19 2.73 3.53 3.05 3.97 兩 (RSD) 略 4% 精 2. 臨 (1) 臨

臨力 103-205 bar 度 307-333K 4 諸 wogonin CO 2 (2) 臨量力 171 bar 度 325K 量量 2 度 2.13 % 量 wogonin 量量論 Lucien Foster 契 11.5% 度力量 3 力 171 bar 度 307-333K wogonin 量度度臨度降 339-342K 量臨度 4 度 325K wogonin 量力力臨度度 Hildebrand 3 4 wogonin 力 171-205bar 度 328-333K 臨 wogonin 力度論臨蓮臨 wogonin (CNPH 93-10) 參 1. (http://www.doh.gov.tw/) 料 2. Chiu, H. F., Lin, C. C., Yang, C. C. and Yang, F., The Pharmacological and Pathological Studies on Several Hepatic Protecttive Crude Drugs from Taiwan(I), American Journal of Chinese Medicine, 16(3-4), 127-137, 1988. 3. Chiu, F. H., Lin, C. C., Yen, M. H., Wu, P. S. and Yang, C. Y., The Pharmacological and Pathological Studies on Several Hepatic Protective Crude Drugs from Taiwan (V): The Effects of Bombax Malabrica and Scutellaria Rivularis, Am. J. Chin. Med., 4, 257-264, 1992. 4. Lin, S. C., Lin, C. C., Lin, Y. H. and Chen, C. H., Protective and Therapeutic Effects of Ban-Zhi-lian on Hepatotoxin-induced Liver Injuries, Am. J. Chin. Med., 22, 29-42, 1994. 5. 林蓮 180 論 73 年度 6. Chou, C. J., A New Favone from Scutellaria Rivularis, J. Taiwan Parm. Assoc., 30, 36-43, 1978. 7. Miyaichi, Y. and Tomimori, T., Studies on the Constituents of Scutellaria Species XVI, On

the Phenol Glycosides of the Root of Scutellaria Baicalensis Georgi, Natural Med., 48, 215-218, 1994. 8. Chou, C. J. and Lin, S. Y., Studies on the Constituents of Scutellaria Rivularis Roots( ), J. Taiwan Parm. Assoc., 37, 186-209, 1985. 9. Tomimori, T., Miyaichi Y., Imoto, Y. and Kizu, H., Studies on the Constituents of Scutellaria Species X, On the Flavonoid Constituents of Ban Zhi Lian the Whole Herb of Scutellaria Rivalaris Wall(I), Shoyakugaku Zasshi, 38, 249-252, 1984. 10. Tomimori, T., Lin, H., Miyaichi, Y., Toyofuku, S. and Namba, T., Studies on the Constituents of Scutellaria Species VI, On the Flavonoid Constituents of the Root of Scutellaria Baicalensis Georgi (5), Quantitative analysis of Flavonoids in Scutellaria Roots by High-performance Liquid Chromatography, Yakugaku Zasshi, 105, 148-155, 1985. 11. Tomimori, T., Miyaichi, Y., Imoto, Y. and Kizu, H., Studies on the Constituents of Scutellaria Species VII, On the Flavonoid Constituents of Ban Zhi Lian, the Whole Herb of Scutellaria Rivalaris Wall(2), Shoyakugaku Zasshi, 40, 432-433, 1986. 12. Tomimori, T., Imoto, Y. and Miyaichi, Y., Studies on the Constituents of Scutellaria Species XII, On the Flavonoid Constituents of Root of Scutellaria Rivularis Wall, Chem. Pharm. Bull., 38(12), 3488-3490, 1990. 13. Kimuya, Y., Kubo, M., Tani, T., Arichi, S. and Okuda, H., Studies on Scutellaria Radix IV, Effects on Lipid Peroxidation in Rat Liver, Chem. Pharm. Bull., 29, 2610-2617, 1981. 14. Abe, K., Inoue, O. and Yumioka, E., Binary Excretion of Metabolites of Baicalin and Baicalein in Rats, Chem. Pharm. Bull., 38(1), 208-211, 1990. 15. Lin, C. C. and Shieh, D. E., The Anti-inflammatory Activity of Scutellaria Rivalaris Extractions and Its Active Components, Baicalin, Baicalein and Wogonin, The 10 th Symposium on Natural Products, Kaohsiung, Taiwan, R.O.C., pp. 94-95, 1995. 16. Lin, C. C. and Shieh, D. E., In Vivo Hepatoprtective Effect of Baicalin, Baicalein and Wogonin from Scutellaria Rivalaris, The Bull. Of Society of Pharmacognosy, R.O.C., NO. IV, pp. 69-70, 1996. 17. 呂 : Baicalein 類 :Shikonin 類論 89 年度 18. 類 Hep G2 cells 滑理論 82 年度 19. 呂 (1) (2) Baicalein 論 89 年度 20. Kobayashi, T., Nakata, T. and Kuzumaki, T., Effect of Favonoids on Cell Cycle Progression in Prostate Cancer Cells, Cancer Lett., Feb 8, 176 (1), 17-23, 2002. 2 1. Hudson, E. A., Dinh, P. A., Kokubun, T., Simmonds, M. S. and Gescher, A., Characterization of Potentially Chemopreventive Phenols in Extracts of Brown rice that Inhibit the Growth of Human Colon Cancer cells, Cancer Epidemiol Biomarkers Prev., Nov, 9(11), 1163-70, 2000. 2 2. 年度 1976 23. Baysal, T. and Starmans, D. A. J., Supercritical carbon dioxide extraction of carvone and limonene from caraway seed, Journal of Supercritical Fluids, 14, 225-234, 1999. 24. Yepez, B., Espinosa, M., Lopez, S. and Bolanos, G., Producing antioxidant fractions from herbaceous matrices by supercritical fluid extraction, Fluid Phase Equilibria, 194-197, 879-884, 2002. 25. Lucas, A., Martinez, E., Rincon, J., Balanco, M. A. and Gracia, I., Supercritical fluid extraction of tocopherol concentrates from olive tree leaves, Journal of Supercritical

Fluids, 22, 221-228, 2002. 26. Chiu, K. L., Cheng, Y. C., Chen, J. H. Chang, C. J. and Yang, P. W., Supercritical fluids extraction of Ginkgo ginkgolides and flavonids, Journal of Supercritical Fluids, 24, 77-87, 2002. 27. Chen, J. H., Xia, Z. H. and Tan, R. X., High-performance liquid chromatographic analysis of bioactive triterpenes in Perilla frutescens, Journal of Pharmaceutical and Biomedical Analysis, 32, 1175-1179, 2003. 28. Lin, M. C., Tsai, M. J.and Wen, K. C., Supercritical fluid extraction of flavonids from Scutellariae Radix, Journal of Chromatography A, 830, 387-395, 1999. 29. Sha, Y. F., Huang, Tao M., Shen, S. and Duan, G. L., Determination of volatile compounds in Magnolia Bark by microwave-assisted extraction couple to headspace solid-phase microextraction and gas chromatography-mass spectrometry, The Japan Society for Analytical Chemistry, 20, 857-859, 2004. 30. Catchpole, O. J., Perry, N. B., da Silva, B. M. T., Grey, J. B. and Smallfied B. M., Supercritical extraction of herbs Ι: Saw Palmetto, St John, s Wort, Kava Root, and Echinacea,, Journal of Supercritical Fluids, 22, 129-138, 2002. 31. Zancan, K. C., Marques, M. O.M., Petenate, A. J., Meireles, M. A. A., Extraction of ginger (Zingiber officinale Roscoe) oleoresin with carbon dioxide and co-solvents: a study of the antioxidant action of the extracts, Journal of Supercritical Fluids, 24, 57-76, 2002. 32. L u q u e d e C a s t r o, M. D. and Te n a, M. T., S t ategies for Supercritical Fluid Extraction of Polar and Ionic Compounds, Trends in Analytical Chemistry, 15, 32-37, 1996. Fig. 1. A schematic diagram of the extraction apparatus of supercritical carbon dioxide.

2.0 Conents of components (mg/g) 1.5 1.0 0.5 :Baicalin :Baicalein :Wogonin 3 6 9 12 Percentage of alcohol (%) Fig. 2. Effect of the amount of co-solvent added on the extraction of three constituents from Scutellaria Barbata Don as function of percentage of alcohol (SCE at 171 bar and 328K). 2.4 Conents of components (mg/g) 1.8 1.2 0.6 :Baicalin :Baicalein :Wogonin 300 310 320 330 340 350 Temperature (K) Fig. 3. Effect of temperature on the extraction of three constituents from Scutellaria Barbata Don (SCE at 171 bar and 11.5% alcohol).

2.4 Contents of components (mg/g) 1.8 1.2 0.6 :Baicalin :Baicalein :Wogonin 80 120 160 200 240 Pressure (bar) Fig. 4. Effect of pressure on the extraction of three constituents from Scutellaria Barbata Don (SCE at 328 K and 11.5% alcohol).