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理 CN9725 糖行 97 年 1 1 97 年 12 31 玲 98 年 2 28

糖廉玲理不女老糖料裡料糖料罹 X, metabolic syndrome 狀異力 oxidative stress 糖料了糖 30% sucrose 糖 250 Wistar (control group, C) 糖 (30% sucrose water group, S) 糖 (pair-fed sucrose group, SP) 8 C S 料 (ad libitum) AIN-93G 料離 7 TBARS 糖降料量 65% 量類例糖 retroperitoneal fat 量 p<0.05 糖料量 55% 兩 60%p<0.05 糖 7 TG C SP 1.64 2.2 p<0.05 糖量降 α- 量 α-/tg 率糖降 GSH 量異糖不 TBARS GSH 量降 α- 度 p<0.05 catalasesod GPx 不糖糖 TBARS 粒 aconitase catalase 量 western blot 論糖 TG 量量糖糖 6 惡 E 狀不降 TBARS catalase 量了糖力糖料例例 60% 糖糖類 metabolic syndrome 狀 (hyperinsulinemia) (hyperlipidemia) (hypertension) 糖糖尿糖糖不力 (oxidative stress) 論糖糖力 (Bar-On & Stein, 1968; Reed et al., 2000) 糖力 (oxidative stress) Faure 1997 利 2 Wistar 糖料 6 TG 不 E TBARS GSSG/GSH 率量降度不 Cu,Zn-SOD 降了 33%Se-GPx 不糖力 20 E 降 TBARS GSSG/GSH 率復 Cu,Zn- SOD 不 TG E 力降力力 Cavarape 2001 Wistar 200-220g 糖 fructose 料糖例量 60% 2 糖 mrna 量異降 catalase mrna Cu,Zn-SOD mrna 降異料糖利率 SODGPx catalase O Dell, B. L. 1993

Busserolles 3 離 Wistar 糖料 2 尿 TBARS E 降異 NOx nitrite+nitrate 量度降量 Cu,Zn-SOD 降 mrna 量 Mn-SOD 粒 GPx catalase mrna 不度降度不 Busserolles et al., 2002a, b 糖兩力 Busserolles et al., 2002c Cu, Zn-SOD 降力糖力 biosensercu, Zn-SOD 兩 Cu ZnCu Zn coactive or cocatalytic role Cu,Zn-SOD 降不量 post-translational level Cu,Zn-SOD 狀 Taylor et al., 1988Chung et al., 1988 H 2 O 2 Cu, Zn-SOD Zn 降 superoxide 力 histidine ligand Cu 降 Sampson and Beckman, 2001 Cu Lynch and Colón,2006 糖力 Cu,Zn-SOD mrna C/EBPα (Kim et al., 1997) 力 Cu,Zn-SOD 錄 mrna 量量降糖 pro-oxidant 力 oxidative stress 不 McDonald, 1995 降量力 Reactive oxygen speices, ROS 力 oxidative stress 糖類糖量來糖類量力糖了量量降了力糖料了糖 30% sucrose 糖料 1. 行 250 Wistar 3 (control group, C) 糖 (30% sucrose water group, S) 糖 (pair-fed sucrose group, SP) 8 C S 料 (ad libitum) 糖量來糖料量降了糖 SP 量糖離 S 糖 or 量降 AIN-93G 料離不行 7 8-12 CO 2 行行 fractionation -70 2. 1 TG 量 RANDOX, Antrim, UK 量 E 0.1 ml 2 ml ( 1% pyrogallol)0.1 ml HCL 6 ml (n-hexane) 烈 5 離 Hexane 5 ml 行冷 200 µl 留 HPLC 量 2EGSH 量 (Superoxide dismutasecu,zn-sod)floheotting, 1984 (Catalase)Aebi, H., 1984 (Glutathione peroxidasegpx)pagliavalentine, 1967 3TBARSMDA (malondialdehyde) 1MDA2TBA(thiobarbituric acid)tbarsthiobarbituric acid reactive substancetbars 量來度 TMP1,1,3,3-tetramethoxypropane MDA 度

4 western blot 行量 5 ± Mean ± SD 數 Duncan s Multiple Range test 來立異 p 0.05 Pearson correlation SAS 行論狀 12 量 1 糖狀力異狀料量降 65% 量類兩異糖 SP 料量 55% 暴不 7 兩 60%p<0.05 量量 2 了例糖量糖 retroperitoneal fat 量 35%p<0.05 量 33%p<0.05EP 糖 SP 了量兩量 p<0.05sp 兩 60% 量量糖不狀糖量例 lipogenesis 量 1 糖量量料利率 C S SP n 8 8 8 Initial body weight (g/rat) 278 ± 7 a 278 ± 7 a 278 ± 7 a Final body weight (g/rat) 496 ± 40 a 504 ± 23 a 308 ± 59 b Total caloric intake (Kcal) 4487 ± 260 a 4750 ± 239 a 2492 ± 520 a Feed efficiency 19.1 ± 2.5 a 30.9 ± 5.0 a 3.5 ± 8.2 a 1. Each value represents Mean ±S.D. 2. Values not sharing the same superscript letters in the same horizontal row are significantly different from on another by Duncan's Multiple Range Test (p<0.05) 3. Feed efficiency = Body weight gain (g) / Food intake (g)

2 糖量量 C S SP (g) n 8 8 8 Liver 13.3 ± 2.1 a 14.3 ± 0.8 a 8.37 ± 2.46 b Kidney 3.26 ± 0.34 a 3.06 ± 0.34 a 2.21 ± 0.38 b Heart 1.29 ± 0.08 a 1.34 ± 0.09 a 0.86 ± 0.13 b Brain 1.80 ± 0.18 a 1.92 ± 0.09 a 1.85 ± 0.10 a epididylmal fat pad (EP) 11.4 ± 3.2 a 10.3 ± 1.6 a 2.96 ± 2.11 b retroperitoneal fat (RE) 12.7 ± 2.2 b 17.2 ± 4.3 a 2.7 ± 2.0 c WAT# 24.1 ± 4.8 a 27.5 ± 4.8 a 6.0 ± 4.0 b Relative tissue weight (%) tissue weight (g) / body weight (g) Liver 2.74 ± 0.27 a 2.92 ± 0.12 a 2.78 ± 0.34 a Kidney 0.68 ± 0.05 b 0.62 ± 0.06 c 0.75 ± 0.03 a Heart 0.27 ± 0.01 b 0.27 ± 0.02 b 0.29 ± 0.02 a Brain 0.37 ± 0.04 b 0.39 ± 0.03 b 0.64 ± 0.13 a epididylmal fat pad (EP) 2.33 ± 0.47 a 2.09 ± 0.26 a 0.93 ± 0.52 b retroperitoneal fat (RE) 2.64 ± 0.42 b 3.5 ± 0.81 a 0.81 ± 0.51 c WAT# 4.98 ± 0.69 a 5.59 ± 0.84 a 1.84 ± 0.97 b 1. Each value represents Mean ±S.D. 2. Values not sharing the same superscript letters in the same horizontal row are significantly different from on another by Duncan's Multiple Range Test (p<0.05) 3. #White adipose tissue, WATepididylmal fat padretroperitoneal fat 3 度異度糖 4 兩 7 64% (p<0.05) 度糖量降 Eα- 度兩 α-/tg 糖 E 糖不 GSH 異 catalasesodgpx 不糖

3 糖 C S SP n 8 8 8 Hemoglobin (mmol/l) 16.7 ± 1.7 a 16.6 ± 1.2 a 16.3 ± 2.7 a Serum RBC TG (mmol/l) 1.07 ± 0.31 b 1.75 ± 0.34 a 0.80 ± 0.23 b α-tocopherol (µmol/l) 11.3 ± 3.6 a 7.7 ± 2.0 b 6.5 ± 3.4 b α-tocopherol/tg ratio (µmol/mmol) 10.9 ± 3.6 a 4.5 ± 1.4 b 8.4 ± 3.3 a GSH (mmol/l) 0.64 ± 0.13 a 0.61 ± 0.11 a 0.64 ± 0.17 a Alkaline phosphatase activity (U/L) 60.0 ± 6.4 a 63.1 ± 11.9 a 72.1 ± 16.7 a Catalase activity (k/µmol Hb) 1.25 ± 0.91 a 1.08 ± 0.35 a 1.28 ± 0.70 a SOD activity (unit/µmol Hb) 67.4 ± 11.1 a 66.6 ±7.2 a 71.4 ± 10.9 a Glutathione peroxidase activity (unit/µmol Hb) 2.55 ± 0.22 a 2.53 ± 0.35 a 2.49 ± 0.34 a 1. Each value represents Mean ±S.D. 2. Values not sharing the same superscript letters in the same horizontal row are significantly different from on another by Duncan's Multiple Range Test (p<0.05) 4 糖不 TBARSGSH 量降 α- 度 p<0.05catalasesodgpx 不糖糖 TBARS 粒 aconitase catalase 量 western blot 降老理論理論了例老.. 歩 4 糖 TBARS C S SP n 8 8 8 TBARS (µmol/g liver) 24.1 ± 3.6 a 22.1 ± 2.0 ab 18.8 ± 4.8 b Mitochondrial aconitase acitivity (unit/mg mitochondrial protein) 5.99 ± 3.2 a 4.46 ± 1.8 ab 3.52 ± 1.1 b Antioxidative molecular GSH (mmol/g liver) 3.53 ± 1.10 a 3.73 ± 0.69 a 4.01 ± 0.52 a α-tocopherol (µmol/ g liver) 15.0 ± 3.4 a 11.6 ± 1.8 b 8.1 ± 1.7 c Antioxidative enzyme Catalase activity (k/µmol Hb) 0.67 ± 0.13 b 0.67 ± 0.11 b 0.87 ± 0.11 a Catalase protein abundance (catalase/β-actin) 1.42 ± 0.38 ab 1.18 ± 0.35 b 1.98 ± 0.83 a SOD activity (unit/µmol Hb) 17.1 ± 2.4 a 17.8 ± 2.1 a 18.1 ±2.1 a Glutathione peroxidase activity (unit/µmol Hb) 0.64 ± 0.10 a 0.67 ± 0.10 a 0.67 ± 0.03 a

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