Korean Chem. Eng. Res., Vol. 44, No. 1, February, 2006, pp. 87-91 치마버섯유래다당체의화상및상처치유효과 Ç h o 200-701 o }e q 2 192-1 (2005 12o 26p r, 2006 1o 31p }ˆ) Effect of Polysaccharide from Schizophyllum commune on Burn and Wound Healing Min Kyoung Kim and Eock Kee Hong Department of Bioengineering & Technology, Kangwon National University, 192-1, Hyoja-2-dong, Chuncheon, Kangwondo 200-701, Korea (Received 26 December 2005; accepted 31 January 2006) k o ~ k l p n n v p. p ~ p }l p k sv r m sv q l l l~ eˆp f o ˆo k v p. np ~ q d n l q svp 2 m p lr p. l l d m l p n o ~ pn l in vivo e k r l } o Š m. l 2 mp o o p o r Š l p p m. h Abstracts Polysaccharides extracted from mushroom have been most commonly used to enhance the immune system. Also polysaccharides maintaining the moisture extent on epidermal tissue have an effect on the removal of necrotic tissue and the restoration of epidermal tissue through enhancing the immune system at skin layers. In this work, polysaccharides were from Schizophyllum commune studied about the burn and wound healing activity in the epidermal tissue on rats through in vivo experiment and hematological values. And antibacterial activities were examined using pathogenic microorganisms causing the secondary inflammation. Key words: Polysaccharide, Burn and Wound Healing, Schizophyllum commune 1. }l qp psp on p l k qnl ~ rp l v l k, t d r, k, p d, m l p l p [1, 2]. o p β-glucanp } ol rp qpqp p v t q pq p p v l q p v eˆ p pv l [3]., o p β-glucanp p l m k q s e p q p eˆ svp pme v p eˆ ( v)p p lr pp 2 mp pp } o p eˆ p p., p o p s, p ˆ p o To whom correspondence should be addressed. E-mail: ekhong@kangwon.ac.kr ve t l v p k r p [4]. q n p v l l r, llrr e p pp p l p r p v l n. m l, } k p v l n p l rm llrr p ~ l p p p. l rp qnp l r l p e, r, v p ppˆ p p o v p m p svl e p r l 2 mp mv pp lr ~ p ql o l p s l l v p eˆ q p. v l r rp v p lr l ql o p p r r p p p k. } p v l pl e m el eˆ p r n p [5-7]. l l p llv ~ pn l } o in vivo e k r l 87
88 Ë l Š mp, Staphylococcus sp. p 2 mp o p p l kk q m. 2. 2-1. m e l n }l p k~ k lkp lˆm r Œ p l rs m. (t) k lp Fucidin (t)ˆ k lp Azulene-Sm p nl k s l rn sl l e l rs l m l } ol p Š m., ~p p rl nl ((t) rk) l n m. } r l e e u l k p H1-system q (Technicon Co.) n m., rl p bacteriap Bacillus megaterium(kctc 3709), Staphylococcus aureus(kctc 1621), Streptococcus pyogenes(kctc 3096)m yeastp Candida albicans (KCTC 7270) pn l e p m. 2-2. i oo k~ k lkp llv ~ r n o ~p 100 mg/mlp ˆpm l p, l l nl 100 µlj l l skrm ~ l, ~ l rs m. 2-3. m lo e p (t)m l 6t (200~250 g)p o Sprague-Dawley k k m 22±3 o C, d 50±5Í, 10~15 /p, 12 h t k, s 150~300 Luxp o l ptp rp e p, (t)m l n p pn m. }p p 7p m. e p e Table 1l ˆ l. 2-4. zm z 6t ratp ol p r, p p pr, 30 l p k l. l p s p k } v kp, s krp Azulene-S, skrm ~ }, ~ } s p p } p l m. l p e (Azulene-S n) } p e (Fucidin n) p p m. p l } pp o p r m. 2-5. n y} h n e rat urethanep eˆ l l k 10 mlp } m. r p } EDTA p r lp tube pn l } m. } r p q pn l k p (white blood cells, WBC), r (red blood cells, RBC), (hemoglobin, Hb), r nr (hematocrit, HCT) Ž (platelet, PLT) r m. 2-6. ~o Bacteriap o v Nutrient Broth(beef extract 3 g/l, peptone 5g/l) n m, yeastp Candida albicansp n glucose(40 g/l), peptone(10 g/l), yeast extract(2 g/l) v pn m. k m Bacillus megateriump 30 o C, bacteriap n 37 o C pl, Candida albicansp n 24 o C pl. r l n s v ~ l v kp v n l p agar plate t m. e v ~p 10 mg/ml, 1 mg/ml, 0.1 mg/ml, 0.01 mg/mlp vl ~ l rs m. r kp ~ l pv kp plate l k t p v 50 mlp kkl single colony rs 12e k k m. kk 100 µl l plate 200~300 p p r p pt 100 µl plate l rs l spreading m. 24e k platel p colony Š l p r m. 3. y 3-1. os {s l p p pp e ratp n, n l r(azulene-s) p } k s o p ~m } e l o skp p okp p m (Fig. 1, Fig. 2). e l v ol mv p mp ve(hyperplasia of epidermis)p pl. s l p plp e l pv kk. v svl o mvp k plp v (sebaceous gland)p l p Ž l. v l p p n s l ee r e l ƒ r opr p l o. e l p r o ˆo p Ž l., } p e l r rp p q l š v s l r(fucidin)m ~ } nl k } v kp e sl r p } e }p o n p pl (Fig. 3). p o p ~ } o Table 1. Grouping of rats for artificial experiments Experimental group scalded o44 o1 2006 2k Normal control No treatment Azulene-S or Fucidin Azulene-S or Fucidin + polysaccharide Vaseline + polysaccharide Experimental group burned 2 4 4 4 4 4 Experimental group wounded 4 4 4 4 4 Vaseline 4 4 4 4 4
~p } o 89 Fig. 1. Experimental groups scalded in the back. A. initial control, B. control after 7 days, C. rat treated with Azulene-S, D. rat treated with Azulene-S+polysaccharide, E. rat treated with vaseline, F. rat treated with Vaseline+polysaccharide(after 7 days). Fig. 3. Experimental groups wounded in the back. A. initial control, B. control after 7 days, C. rat treated with Fucidin, D. rat treated with Fucidin+polysaccharide, E. rat treated with vaseline, F. rat treated with vaseline+polysaccharide(after 7 days). l e n qpqp p ve ~ p p β-glucanp }svp l pp eˆ p Ž [8]., } ol do p s l 2 mp lr p f } ol rpl Ž. Fig. 2. Experimental groups burned in the back. A. initial control, B. control after 7 days, C. rat treated with Azulene-S, D. rat treated with Azulene-S+polysaccharide, E. rat treated with vaseline, F. rat treated with vaseline+polysaccharide(after 7 days). 3-2. h n p p e l ~ pn l l r } up k j v dp m (Table 2). p l r p mv o p pv, p r op 6.60~12.6 10 3 /µll l v kpp p p l., r m p n l rp Azulene-Sp } nl k p pl, p o svp p p Ž lp [9], Žp ~rp r o ov p p ˆ. p p e p n, r o l n pp r rp r o ov m. t } e l e pp l mv p pp m, e p r m Ž, r tpl. } p e p n etl n p l r(fucidin) } Korean Chem. Eng. Res., Vol. 44, No. 1, February, 2006
90 Ë l Table 2. Hematological value of the rats during burn and wound experimental periods Groups WBC ( 10 3 /µl) RBC ( 10 3 /µl) Hb (g/dl) HCT ( ) PLTG( 10 3 /µl) Normal range 6.6~12.6 6.7~9.75 13.4~15.8 44.4~50.4 150~450 Standard group Normal control 9.02±0.42 9.45±0.71 11.3±0.25 39.6±1.13 203.5±2.121 Experimental group Control 5.66±1.10 6.61±2.67 1.10±3.81 142.1±16.26 1263.5±108.18 scalded Azulene-S 8.47±9.91 4.91±5.07 18.5±8.76 132.1±33.79 181.5±24.74 Vaseline 7.62±0.14 19.3±1.28 13.5±2.12 57.3±7.91 1.342±24.04 Azulene-S+polysaccharide 11.32±1.811 7.43±1.49 13.3±1.34 148.1±12.58 1.1217±101.11 Vaseline+polysaccharide 8.08±2.88 9.35±0.04 13.2±1.13 55.4±2.19 1.334±36.06 Experimental group Control 9.07±0.91 7.20±2.93 10.5±3.81 143.2±14.14 11.247±185.26 burned Azulene-S 10.43±0.181 9.47±0.38 14.5±0.84 58.45±4.731 351.5±4.941 Vaseline 13.94±2.311 8.56±0.74 14.4±0.00 45.1±8.62 281.5±36.06 Azulene-S+polysaccharide 13.09±2.501 6.97±0.48 1.13±0.42 44.55±2.191 1195.5±120.91 Vaseline+polysaccharide 12.75±0.631 8.46±0.96 13.9±0.42 1.61±1.41 1.176±11.31 Experimental group Control 7.18±1.86 7.68±0.14 13.55±0.071 41.6±0.14 215.5±4.941 wounded Fucidin 14.55±3.261 6.87±0.36 12.1±1.41 37.55±0.911 1.234±25.45 Vaseline 19.2±0.70 7.34±0.04 14.2±0.14 40.65±0.071 314.5±2.121 Fucidin+polysaccharide 7.85±1.73 7.73±0.30 12.6±0.14 42.6±0.70 1.269±67.88 Vaseline+polysaccharide 7.72±2.32 6.95±0.31 12.65±0.351 39.2±0.28 277.5±54.44 Table 3. Antibacterial activity of polysaccharide in agar plate ColonyW Control a b c d # Strains Colony # Colony # IR( ) Colony # IR( ) Colony # IR( ) Colony # IR( ) Bacillus megaterium 2.0 10 2 0.11 10 2 94.5 0.16 10 2 92 0.38 10 2 81 0.92 10 2 54 Staphylococcus aureus 90 11 88 28 69 34 63 32 65 Streptococcus pyogenes 5.1 10 3 1.2 10 3 77 1.5 10 3 71 2.6 10 3 50 2.8 10 3 45 Candida albicans 1.2 10 3 3.2 10 2 74 3.4 10 2 72 4.0 10 2 67 7.2 10 2 40 *Control: medium not including polysaccharide a. Polysaccharide concentration: 10 mg/ml, b. Polysaccharide concentration: 1 mg/ml, c. Polysaccharide concentration: 0.1 mg/ml, d. Polysaccharide concentration: 0.01 mg/ml *IR: Inhibition rate( ) e p r o r l mv o [10]p p l l ~ pn l l n nl, r m Ž, r o ov p p m. 3-3. ~ agar platel q p colony ~ v kp control platel p colony r ˆ p plp, ~p kp p o vp p colony r ˆ p pl (Table 3). Staphylococcus aureusm Streptococcus pyogenesp n v ~p 0.1 mg/ml, 0.01 mg/mlp platel p colony p p l ˆ v kv, ~ o n n n p qp p ˆ., Bacillus megateriumm Candida albicansp nl r~rp controll l qp r l p k pl [11]. 4. }l p } p r p p p o e rat pn l e r } p o44 o1 2006 2k in vivo e m k r p ee l } o Š m. n l p } p u o p ~ o l p l o, skr n k } v kp s l l } o lpp l pl. } p u p p v k p p plp, l o p p pl. p p e p } o s kr l ~p d l p p Ž. k r, r~rp o p ~ l } e p n kp r ol l v k pp k plp, }o e l mv p p rp p p pl. p f ~ l sp l r }l o m 2 mp m l ppp p m. mvp o p p ~p kk, ~ o l p vl q p s l qp r k. Bacillus megaterium Candida albicansp qp e r p e p l. rp o ~ } o k m ˆ p p pl.
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