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理葉 -- 猪粒葉類 CNHN-93-01 行 93 年 01 01 93 年 12 31 瑩林理參行理 94 年 2 28 1

理葉 -- 猪粒葉類 CNHN-93-01 行 93 年 01 01 93 年 12 31 瑩理林理理 E-mail: shihying@mail.chna.edu.tw (Morinda citrifolia) Rubiaceae Noni 療料葉糖尿療療葉力葉精 LYD 猪粒力力類粒 Morinda citrifolia Rubiaceae, commonly known as Noni, is a plant typically found in the Hawaiian, Tahitian and tropical Asia. The bark, stem, roots, leaves, and fruits have been used traditionally as a folk remedy for many diseases including diabetes, hypertension, and cancer. This study was to prove the leaves of Noni crude extracts in the protection ability of biological cell membrane which were attacted by oxidative damage. The results indicated that the absolute ethanol extracts of display significantly the inhibition ability of lipid peroxidation of microsomes from LYD pig liver. The scavenging mechanism of the absolute ethanol extracts of might have relation with hydroxyl radical. The antioxidant components might be attributed to higher content of polyphenolic compounds. Keywords: Morinda citrifolia, Noni, lipid peroxidation, microsomes. Free radical Reactive oxygen species 年來理糖尿老 1 Morinda citrifolia Rubiaceae Noni 2 22 2

易類不料量年來 Hirazumi, 1994 3 1996 4 Lewis lung carcinoma Liu Sang 葉 5,6 Hirazumi 1999 類 Polysaccharide-rich substance immunomodulator 理 7 Sang 8 葉來 Flavonol glycosides Zin 2002 年葉 9 10-16 葉 in vitro 不論 80 50% 99.5% 精臨 SF-CO 2 都不不了更葉猪粒猪易力類立猪粒料料 Morinda citrifolia 葉冷列 1 2 99.5%EtOH 3 50%EtOH 4 80 Germano 2002 17 說行 (1) 猪粒離 3 30~40 Kg LYD 猪 500-600 g 冷 1.15% KCl 0.01M KH 2 PO 4 (ph 7.4, 4 ) 4 20 w/v 離 20 w/v 4 670 g 離 10 離 4 C 100,00 g 離 15 粒離離 (4 C 105,000 g, 60 ) 粒粒 1~2 ml 粒粒 90 1.5 粒行量 (2) 1ml 粒 0.5mg protein ml FeSO 4 ( 度 10 µm) ascorbic acid ( 度 0.1 mm) 37 (180 rpm) 0.5 ml 1 ml TCA-TBA-HCl (15%w/v TCA, 0.375% w/v TBA, 0.25N HCl) 15 冷冷 8,000 g 離 10 532nm (3) 3

行數 SAS/PC 行 ANOVA 異數 Duncan s multiple range tests 異論葉猪粒度力度 125 µg/ml 葉 99.5% 50% 90 34.7% 29.0% 葉力若 50% 粒度 (EC 50 ) Trolox 6.81 0.54 µg/ml 葉 99.5% 50% 28.55 6.83 90.09 0.95 µg/ml 粒 EC 50 Trolox 4 13 10-16 葉不力葉力葉 O 2 - H 2 O 2 力葉力葉葉類類量參葉類類 Inhibition effect on lipid peroxidation(%) 120 100 80 60 40 20 0 0 200 400 600 Concentration(µg/ml) EtoAc;50 99.5%EtOH; 50 50%EtOH; 50 H2O; 80 Trolox 葉不猪粒 Figure 1. Effect of crude extracts by various solvent extractions on lipid peroxidation from pig liver microsome. All samples were tested at five to eight concentrations and each value was means of three replicate analysis. 葉不猪粒 EC 50 Table 1. EC 50 of crude extracts by various solvent extractions on lipid peroxidation from pig liver microsome. Sample EtOAc ; 50 ) (99.5% EtOH ; 50 ) (50%EtOH ; 50 ) (H 2 O ; 80 ) Trolox EC 50 (µg/ml) 226.00±13.93 e 28.55±6.83 b 90.09±0.95 c 164.77±4.42 d 6.81±0.54 a 1. Each value is the mean±sd (n=3). 2. EC 50 the efficient concentration of crude Noni extracts caused 50% inhibition effects on lipid peroxidation. 4

1. 葉 2. 立猪粒易 3. 葉精 LYD 猪粒力力類六參 1. Gutteridge J. M. C., and Halliwell B., Antioxidants in nutrition health and disease, 1th edition, Oxford: Oxford University Press, 1994. 2. Sang S., Cheng X., Zhu N., Stark RE., Badmaev V., Ghai G., Rosen RT. and Ho CT. 2001 Flavonol glycosides and novel iridoid glycoside from the leaves of Morinda citrifolia. J.Agric.& Food Chem. 49(9):4478-81. 3. Hirazumi A., Furusawa E., Chou SC. and Hokama Y., Anticancer activity of Morinda citrifolia (noni) on intraperitoneally implanted Lewis lung carcinoma in syngeneic mice. Proceedings of the Western Pharmacology Society, 37: 145-6, 1994. 4. Hirazumi A., Furusawa E., Chou S. C. and Hokama Y., Immuno -modulation contributes to the anticancer activity of Morinda citrifolia (noni) fruit juice. Proceedings of the Western Pharmacology Society, 39: 7-9, 1996. 5. Liu G., Bode A., Ma WY., Sang S., Ho CT. and Dong Z., Two novel glycosides from the fruits of Morinda citrifolia (noni) inhibit AP-1 transactivation and cell transformation in the mouse epidermal JB6 cell line. Cancer Research, 61(15): 5749-56, 2001. 6. Sang S., He K., Liu G., Zhu N., Cheng X., Wang M., Zheng Q., Dong Z., Ghai G., Rosen RT. and Ho CT., A new unusual iridoid with inhibition of activator protein-1 (AP-1) from the leaves of Morinda citrifolia L. Organic Letters, 3(9): 1307-9, 2001. 7. Hirazumi A. and Furusawa E., An immunomodulatory polysaccharide-rich substance from the fruit juice of Morinda citrifolia (noni) with antitumour activity. Phytotherapy Research, 13(5): 380-7, 1999. 8. Sang S., Cheng X., Zhu N., Stark RE., Badmaev V., Ghai G., Rosen RT. and Ho CT., Flavonol glycosides and novel iridoid glycoside from the leaves of Morinda citrifolia. J.Agric.& Food Chem., 49(9): 4478-81, 2001. 9. Zin ZM., Abdul-Hamid A., and Osman A., Antioxidative activity of extracts from Mengkudu (Morinda citrifolia L.) root,fruit and leaf. Food Chemistry, 78: 227 231, 2002.Chen, S. Y., Chen C. H., Lin T. P. and Yeh D. B. 2004 Comparison of antioxidative activity of various crude extracts from leaves, stems and fruits of Morinda citrifolia. Journal of food and drug analysis in manuscript. 10. Chen, S. Y., Chen C. H., Wan Y. C., Chiang C. H., Chung Y. L. and Yeh D. B. 2003 Evaluation of antioxidative activity in Noni leaf extracts. Chia Nan Annual Bulletin 29:87-96. 11. Chen, S. Y., T. P. Lin2, Chung Y. L. and Yeh D. B. 2004 Studies on the antioxidant activity of crude extracts from Noni leaves 12 p114. 12. 玲林葉瑩 * 2004 年 13. 瑩 2004 Antioxidants purification and antioxidative physiological properties of Morinda citrifolia 行 14. 瑩 2003 行 15. 瑩葉 2003 Morinda citrifolia 理 16. 玲瑩 2004 Comparison of Antioxidative Activity of Crude Extracts from Leaves, Stems and Fruits of Morinda citrifolia (Noni). Department of Biotechnology Chia-Nan University of Pharmacy and Science. Thesis for the Degree of Master. 17. Germano, M. P., et al., Evaluation of Extracts and Isolated Fraction from Capparis spinosa L. Buds as an Antioxidant Source. J. Agric. Food Chem.,50(5); 1168-1171, 2002. 5