理 (3) 類 CNPH93-01 行 93 年 1 1 93 年 12 31 切 蘭 參 劉 李 林 嵐 行 94 年 2 27 0
不 了 酪 (tyrosine) 酪 列 兩 eumelanin phaeomelanin (1) 酪 率 (2) 了酪 參 兩 酪 (tyrosine related-protein; TRP) TRP-1 (DHICA oxidase) TRP-2 (dopachrome tautomerase) (3) 數 度 (hyperpigmentation) 泌 力 力 降 都 度 若 度 了 (4) 留 落 落 理 若 率 率 落 來 說 流行 來 例 來 率 數 若 理不 數 度 酪 量來 A (retinolsalicylic acid A 裂 來 滑 (5) 都 類 magnesium ascorbyl phosphate kojic acid ascorbyl glucoside arbutin chamomile ET sodium ascorbyl phosphate ellagic acid 易 不易 療 (6) (7) (8) (arbutin) bearberry 酪 了 (9) (kojic acid) Aspergillus Penicillium 煉 離 酪 降 酪 (10) 1
Endothelin 1 (ET1) 酪 TRP-1 力 (11) chamomile ET ET1 數 都 路 酪 路 率 酪 (12) 來 酪 歷 療 不 利 酪 不 不見 便 了 說 鶴 蓮 說 異 酪 酪 in vitro 96 洞 洞 70µl HBSS 10µl 不 度 蓮 10µl 2U 酪 37 C 30 10µl 10 mm L-dopa 5 492nm 來 量 (12) 參 Jiao 1992 年 (13) MTT 粒 succinate dehydrogenase 藍 formazan DMSO formazan 藍 550nm 便 率 (B16-F0 cells) 10% FBS 2mM L-glutamine 100µg/ml penicillin/streptomycin 1% non-essential amino acid 3.7g/l NaHCO 3 ph 7.2~7.4 DMEM 行 100µl 96 洞 6 10 3 cells/well 24 不 度 蓮 100µl 72 HBSS MTT 0.5mg/ml 100µl 2.5 DMSO 藍 ELISA reader 讀 550nm 理不 度 蓮 量 (melanin content assay) 利 藍 數 數 來 量 2
數 離 pellet 1 HBSS 離 離 6,000g 10 pellet 0.5 1N NaOH 30 ; 30 離 200µl 96 洞 415nm 來 量 (14) 論 蓮 酪 蓮 Eclipta prostrata L. ( 暈 痢 神 療 (15) 蓮 coumestans 類 wedelolactone dimethylwedelolactone (16) 蓮 cyclophosphamide hydrocortisone 老 力 降 力 (17) 蓮 wedelolactone trypsin phospholipase A2 (18) 蓮 酪 力 蓮 易 不 更 來 蓮 離 參 1. Ito, S., Wakamatsu, K., and Ozeki, H. Chemical analysis of melanins and its application to the study of the regulation of melanogenesis. Pigment Cell Res., 13 (suppl. 8), 103-109, 2000. 2. Hearing, V.J. and Jimenez, M. Mammalian tyrosinase the critical regulatory point in melanocyte pigmentation. Int. J. Biochem., 19, 1141-1147, 1987. 3. Boissy, R.E., Sakai, C., and Zhao, H., Kobayashi, T., Hearing, V.J. Human tyrosinase related protein-1(trp-1) does not function as a DHICA oxidase activity in contrast to murine TRP-1. Exp. Dermatol., 7, 198-204, 1998. 4. Jimbow, K. and Minamitsuji, Y. Topical therapies for melasma and disorders of hyperpigmentation. Dermatol. Ther., 14, 35-45, 2001. 5. Pathak, M.A., Fitzpatrick, T.B., and Kraus, E.W. Usefulness of retinoic acid in the treatment of melasma. J. Am. Acad. Dermatol., 15, 894-899, 1986. 6. Mumma, R.O., Mckee, E.E., and Verlangieri, A.J. Anti-ascorbutic effect of ascorbic acid 2-sulfate in the guinea pig. Nutr. Rep. Int., 6, 133-137, 1972. 7. Manning, J.M. and Meister, A. Conversion of praline to collagen hydroxyproline. Biochemistry, 5, 1154-1165, 1966. 8. Hayakawa, R. Effects of combination preparation of vitamine E and C in comparison with single preparation to the patients of facial hyperpigmenation: a double-blind led clinical trial. Nishinihon. J. Dermatol., 42, 1024-1034, 1980. 9. Maeda, K. and Fukuda, M. Arbutin: mechanism of its depigmenting action in human melanocyte culture. Pharmacol. & Experimental Ther. 276, 765-769, 1996. 10. Briganti, S., Camera, E., and Picardo, M. Chemical and instrumental approaches to treat hyperpigmentation. Pigment Cell Res. 16, 101-110, 2003. 11. Imokawa, G., Kobayashi, T., Miyagishi, M., Higashi, K., and Yada, Y. The role of endothelin-1 in epidermal hyperpigmentation and signaling mechanisms of mitogenesis and melanogenesis. Pigment Cell Res., 10, 218-28, 1997. 12. Choi, S.Y., Kim, S., Kim, H., Suk, K., Hwang, J.S., Lee, B.G., Kim, A.J., and Kim, S.Y. (4-Methoxy-benzylidene)-(3-methoxy-phenyl)-amine, a nitrogen analog of stilbene as a potent inhibitor of melanin production. Chem. Pharm. Bull., 50, 450-452, 2002. 3
13. Jiao, H., Soejima, Y., Ohe, Y., and Saijo, N.A. A new MTT assay for examining the cytotoxicity of activated macrophages towards the nonadherent P388 leukemia cell line. J. Immnol. Methods., 153, 265-266, 1992. 14. Kim, D.S., Kim, S.Y., Chung, J.H., Kim, K.H., Eun, H.C., and Park, K.C. Delayed ERK activation by creamide reduces melanin synthesis in human melanocytes. Cellular Sig., 14, 779-785, 2002. 15. 年,, (5), 北, p.231-232, 1983. 16. Saxena, A. X., Singh, B., and Anand, K. K. Hepatoprotective effects of Eclipta alba on subcellular levels in rats. J. Ethanopharmacol., 40, 155-161, 1993. 17. Jayathirtha, M. G. and Mishra, S. H. Preliminary immunomodulatory activities of methanol extracts of Eclipta alba and Centella asiatica. Phytomedicine, 11, 361-365, 2004. 18. Melo, P. A. and Ownby, C. L. Ability of wedelolactone, heparin, and para-bromophenacyl bromide to antagonize the myotoxic effects of two crotaline venoms and their PLA2 myotoxins. Toxicon., 37, 199-215, 1999. 0.5 年 年 tynosinase:u/µl 異 異 dopa:1m 4
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