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그림 1-4 -
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그림 3 농도에따른댓잎추출액의항세균효과 - 6 -
그림 4 한천확산법 그림 5 배지에균뿌리는과정 그림 6 배지에균스프레딩하는과정 표 1 실험에사용한미생물의종류와배양에사용한배지 Bacterial Strain Gram (+) bacteria Rothia dentocariosa G1201 Streptococcus mutans CCARM 0079 Bacillus subtilis Staphylococcus saprophyticus Staphylococcus epidermis Media for Cultivation Nutrient media (TSA), 30 C, Aerobic Brain Heart Infusion (BHI), 37 C, Aerobic Gram (-) bacteria Escherichia coli JM109-7 -
Serratia marcescens Klebsiella pneumoniae Salmonella typhimurium Lactic Acid Bacteria (LAB) Lactobacillus paraplantarum GL Lactobacillus casei LS2 Lactobacillus plantarum M1 Lactobacillus fermentum JS1101 Leuconostoc gasicomitatum MB Leuconostoc citreum IH22 Leuconostoc kimchii IH25 그림 7 그림 8 Gram (+) bacteria Strain 표 2 댓잎추출물에의한성장억제효과 Optical density (1) (1unit) Inhibition zone on agar plate (mm) (2) (1unit) Inhibition zone on agar plate (mm) (2) (10 unit) Rothia dentocariosa G1201 0.038 33.5 33.5 Streptococcus mutans CCARM0079 0.072 22 22.5 Staphylococcus epidermis 0.126 17.5 17 Bacillus subtilis 0.142 20 21.5-8 -
Staphylococcus saprophyticus 0.171 22.5 22 Gram (-) bacteria Escherichia coli JM109 0.092 12.5 13.5 Serratia marcescens 0.103 20 20 Salmonella typhimurium 0.110 15.5 16 Klebsiella pneumoniae 0.145 17.5 15.5 Lactic Acid Bacteria (LAB) Lactobacillus fermentum JS1101 0.764 ㅡ (3) ㅡ Leuconostoc citreum IH22 0.979 ㅡㅡ Lactobacillus paraplantarum GL 0.988 ㅡ ㅡ Leuconostoc kimchii IH25 0.991 ㅡ ㅡ Lactobacillus plantarum M1 0.992 ㅡ ㅡ Lactobacillus casei LS2 1.012 ㅡ ㅡ Leuconostoc gasicomitatum MB 1.022 ㅡ ㅡ (1) Optical density at 540nm. (2) Diameter of inhibition/clear zone. (3) Very weak or no inhibitory zone was formed. 표 3 황산암모늄침전물에의한항균작용을검사하기위한미생물의배양조건 Strain Gram (+) bacteria Rothia dentocariosa Streptococcus mutans CCARM0079 Bacillus subtilis Staphylococcus saprophyticus Staphylococcus epidermis Culture condition Nutrient media (TSA), 30 C, Aerobic Brain Heart Infusion (BHI), 37 C, Aerobic - 9 -
Gram (-) bacteria Escherichia coli JM109 Serratia marcescens Klebsiella pneumoniae Salmonella typhimurium Lactic Acid Bacteria (LAB) Lactobacillus paraplantarum GL Lactobacillus casei LS2 Lactobacillus plantarum M1 Lactobacillus fermentum JS1101 Leuconostoc gasicomitatum MB Leuconostoc citreum IH22 Leuconostoc kimchii IH25 표 4 여러종류의미생물들에대한성장억제실험의결과 Gram (+) bacteria Strain Optical density (1) Inhibition zone on agar plate (mm)* (2) Rothia dentocariosa G1201 0.028 25.5 ± 0.25 Streptococcus mutans CCARM0079 0.058 23 ± 0.73 Staphylococcus epidermis 0.142 12 ± 0.50 Gram (-) bacteria Staphylococcus saprophyticus 0.171 17.5 ± 0.87 Lactic Acid Bacteria (LAB) Bacillus subtilis 0.898 ㅡ (3) Escherichia coli JM109 0.071 13 ± 0.43 Salmonella typhimurium 0.141 16.5 ± 0.71 Klebsiella pneumoniae 0.186 11.5 ± 0.50 Serratia marcescens 0.925 ㅡ Lactobacillus fermentum JS1101 0.837 ㅡ Leuconostoc citreum IH22 0.912 ㅡ Lactobacillus paraplantarum GL 0.948 ㅡ Leuconostoc kimchii IH25 0.951 ㅡ Lactobacillus plantarum M1 0.984 ㅡ Lactobacillus casei LS2 1.010 ㅡ Leuconostoc gasicomitatum MB 1.036 ㅡ * Values are means ± SD (mm) of three separate experiments (1) Optical density at 540nm. (2) Diameter of inhibition/clear zone. (3) Very weak or no inhibitory zone was formed. - 10 -
표 5 황산암모늄침전물에상등액의첨가에의한항균효과의회복 Gram (+) bacteria Strain Optical density (1) (1 unit) Inhibition zone on agar plate (mm) (2) (1 unit) Inhibition zone on agar plate (mm) (2) (10 unit) Rothia dentocariosa G1201 0.038 33.5 ± 0.50 33.5 ± 0.43 Streptococcus mutans CCARM 0079 0.072 22 ± 0.25 22.5 ± 0.71 Gram (-) bacteria Staphylococcus epidermis 0.126 17.5 ± 0.43 17 ± 0.25 Bacillus subtilis 0.142 20 ± 0.87 21.5 ± 0.83 Staphylococcus saprophyticus 0.171 22.5 ± 0.50 22 ± 0.20 Lactic Acid Bacteria (LAB) Escherichia coli JM109 0.092 12.5 ± 0.83 13.5 ± 0.83 Serratia marcescens 0.103 20 ± 0.71 20 ± 0.25 Salmonella typhimurium 0.110 15.5 ± 0.50 16 ± 0.50 Klebsiella pneumoniae 0.145 17.5 ±0.25 15.5 ± 0.73 Lactobacillus fermentum JS1101 0.764 ㅡ (3) ㅡ Leuconostoc citreum IH22 0.979 ㅡ ㅡ Lactobacillus paraplantarum GL 0.988 ㅡ ㅡ Leuconostoc kimchii IH25 0.991 ㅡ ㅡ Lactobacillus plantarum M1 0.992 ㅡ ㅡ Lactobacillus casei LS2 1.012 ㅡ ㅡ Leuconostoc gasicomitatum MB 1.022 ㅡㅡ * Values are means ± SD (mm) of three separate experiments (1) Optical density at 540nm. (2) Diameter of inhibition/clear zone. (3) Very weak or no inhibitory zone was formed. - 11 -
` 그림 9 acrylamide gel 만드는과정 그림 10 전기영동과정 그림 11 단백질전기영동결과 - 12 -
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그림 12 Streptococcus mutan 에대한댓잎의항세균효과 - 15 -
그림 13 CT-26 세포에대한댓잎의효과 - 16 -
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