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한수지 48(3), , 2015 Original Article Korean J Fish Aquat Sci 48(3), ,2015 전복 (Haliotis discus hannai) 용배합사료내오징어분및해조류대체원으로서대두박이전복치패의성장과체조성에미

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<110> Super-Bio Co., Ltd. KWON, Suk-Tae <120> Thermostable DNA Polymerase-encoding Gene from Thermus sp. X-1 an d Amino Acid Sequence Deduced Therefrom <130> 01-0001 <160> 5 <170> KOPATIN 1.5 <210> 1 <211> 2694 <212> DNA <213> Thermus sp. X-1 <220> <221> CDS <222> (97)..(2589) <400> 1 aagcttcccg cagaggaaag ggaggctttt tggcaggagc tcagggacct cacccgggga 60 caggtgcgct gggaaggcta gggtagcata agggcg atg ctg ccc ctc ttt 111 Met Leu Pro Leu Phe 1 5 gag ccc aag ggc cgg gtg ctt ctg gtg gac ggc cac cac ctg gcc tac 159 Glu Pro Lys Gly Arg Val Leu Leu Val Asp Gly His His Leu Ala Tyr 10 15 20 cgt acc ttt ttt gcc ctg aag ggc ctc acc acc agc cgc ggg gag ccg 207 Arg Thr Phe Phe Ala Leu Lys Gly Leu Thr Thr Ser Arg Gly Glu Pro 25 30 35 gtc cag gcg gtg tac ggg ttt gcc aag agc ctt ttg aag gcg cta agg 255 Val Gln Ala Val Tyr Gly Phe Ala Lys Ser Leu Leu Lys Ala Leu Arg 40 45 50 gaa gac ggg gat gtg gtg atc gtg gtg ttt gac gcc aag gcc ccc tcc 303 Glu Asp Gly Asp Val Val Ile Val Val Phe Asp Ala Lys Ala Pro Ser 55 60 65 ttc cgc cac cag acc tac gag gcc tac aag gcg ggg cgg gct ccc acc 351 Phe Arg His Gln Thr Tyr Glu Ala Tyr Lys Ala Gly Arg Ala Pro Thr - 12 -

70 75 80 85 ccc gag gac ttt ccc cgg cag ctt gcc ctt atc aag gag atg gtg gac 399 Pro Glu Asp Phe Pro Arg Gln Leu Ala Leu Ile Lys Glu Met Val Asp 90 95 100 ctt ttg ggc ctg gag cgc ctc gag gtg ccg ggc ttt gag gcg gat gac 447 Leu Leu Gly Leu Glu Arg Leu Glu Val Pro Gly Phe Glu Ala Asp Asp 105 110 115 gtc ctg gct acc ctg gcc aag aag gcg gaa aag gaa ggc tac gag gtg 495 Val Leu Ala Thr Leu Ala Lys Lys Ala Glu Lys Glu Gly Tyr Glu Val 120 125 130 cgc atc ctc acc gcg gac cgg gac ctt tac cag ctt ctt tcg gag cga 543 Arg Ile Leu Thr Ala Asp Arg Asp Leu Tyr Gln Leu Leu Ser Glu Arg 135 140 145 atc tcc atc ctt cac ccg gag ggt tac ctg atc acc ccg gag tgg ctt 591 Ile Ser Ile Leu His Pro Glu Gly Tyr Leu Ile Thr Pro Glu Trp Leu 150 155 160 165 tgg gag aag tat ggg ctt aag cct tcc cag tgg gtg gac tac cgg gcc 639 Trp Glu Lys Tyr Gly Leu Lys Pro Ser Gln Trp Val Asp Tyr Arg Ala 170 175 180 ttg gcc ggg gac cct tcc gac aac atc ccc ggc gtg aag ggc atc ggg 687 Leu Ala Gly Asp Pro Ser Asp Asn Ile Pro Gly Val Lys Gly Ile Gly 185 190 195 gag aag acg gcg gcc aag ctg atc cgg gag tgg gga agc ctg gaa aac 735 Glu Lys Thr Ala Ala Lys Leu Ile Arg Glu Trp Gly Ser Leu Glu Asn 200 205 210 ctt ctt aag cac ctg gaa cag gtg aaa cct gcc tcc gtg cgg gag aag 783 Leu Leu Lys His Leu Glu Gln Val Lys Pro Ala Ser Val Arg Glu Lys 215 220 225 atc ctt agc cac atg gag gac ctc aag cta tcc ctg gag cta tcc cgg 831 Ile Leu Ser His Met Glu Asp Leu Lys Leu Ser Leu Glu Leu Ser Arg 230 235 240 245 gtg cgc acg gac ttg ccc ctt cag gtg gac ttc gcc cgg cgc cgg gag 879 Val Arg Thr Asp Leu Pro Leu Gln Val Asp Phe Ala Arg Arg Arg Glu 250 255 260 ccg gac cgg gag ggg ctt aag gcc ttt ttg gag agg ctg gag ttc gga 927 Pro Asp Arg Glu Gly Leu Lys Ala Phe Leu Glu Arg Leu Glu Phe Gly 265 270 275 agc ctc ctc cac gag ttc ggc ctg ttg gaa agc ccg gtg gcg gcg gag 975 Ser Leu Leu His Glu Phe Gly Leu Leu Glu Ser Pro Val Ala Ala Glu 280 285 290 gaa gct ccc tgg ccg ccc ccc gag gga gcc ttc gtg ggg tac gtt ctt 1023 Glu Ala Pro Trp Pro Pro Pro Glu Gly Ala Phe Val Gly Tyr Val Leu 295 300 305 tcc cgc ccc gag ccc atg tgg gcg gag ctt aac gcc ttg gcc gcc gcc 1071 Ser Arg Pro Glu Pro Met Trp Ala Glu Leu Asn Ala Leu Ala Ala Ala 310 315 320 325 tgg gag gga agg gtt tac cgg gcg gag gat ccc ttg gag gcc ttg cgg 1119 Trp Glu Gly Arg Val Tyr Arg Ala Glu Asp Pro Leu Glu Ala Leu Arg 330 335 340 ggg ctt ggg gag gtg agg ggg ctt ttg gcc aag gac ctg gcg gtg ctg 1167-13 -

Gly Leu Gly Glu Val Arg Gly Leu Leu Ala Lys Asp Leu Ala Val Leu 345 350 355 gcc ctg agg gaa ggg att gcc ctg gca ccg ggc gac gac ccc atg ctc 1215 Ala Leu Arg Glu Gly Ile Ala Leu Ala Pro Gly Asp Asp Pro Met Leu 360 365 370 ctc gcc tac ctc ctg gat cct tcc aac acc gcc ccc gaa ggg gta gcc 1263 Leu Ala Tyr Leu Leu Asp Pro Ser Asn Thr Ala Pro Glu Gly Val Ala 375 380 385 cgg cgc tac ggg ggg gag tgg acc gag gag gcg ggg gaa agg gcg ttg 1311 Arg Arg Tyr Gly Gly Glu Trp Thr Glu Glu Ala Gly Glu Arg Ala Leu 390 395 400 405 ctt tcc gaa agg ctt tac gcc gcc ctc ctg gag cgg ctt aag ggg gag 1359 Leu Ser Glu Arg Leu Tyr Ala Ala Leu Leu Glu Arg Leu Lys Gly Glu 410 415 420 gag agg ctt ctt tgg ctt tac gag gag gtg gaa aag ccc ctt tcg cgg 1407 Glu Arg Leu Leu Trp Leu Tyr Glu Glu Val Glu Lys Pro Leu Ser Arg 425 430 435 gtc ctg gcc cac atg gag gcc acg ggg gta cgg ttg gat gtg gcc tac 1455 Val Leu Ala His Met Glu Ala Thr Gly Val Arg Leu Asp Val Ala Tyr 440 445 450 tta aag gcc ctt tcc ctg gag gtg gag gcg gag ctc agg cgc ctc gag 1503 Leu Lys Ala Leu Ser Leu Glu Val Glu Ala Glu Leu Arg Arg Leu Glu 455 460 465 gag gag gtc cac cgc ctg gcc ggg cat cct ttc aac ctg aac tcc cgg 1551 Glu Glu Val His Arg Leu Ala Gly His Pro Phe Asn Leu Asn Ser Arg 470 475 480 485 gac cag ctg gaa agg gtc ctc ttt gac gag ctt ggg ctt ccc gcc atc 1599 Asp Gln Leu Glu Arg Val Leu Phe Asp Glu Leu Gly Leu Pro Ala Ile 490 495 500 ggc aag acg gag aag acg ggc aag cgc tcc acc agc gcc gcc gtt ttg 1647 Gly Lys Thr Glu Lys Thr Gly Lys Arg Ser Thr Ser Ala Ala Val Leu 505 510 515 gag gcc ttg cgg gag gct cat ccc atc gtg gac cgc atc ctt cag tac 1695 Glu Ala Leu Arg Glu Ala His Pro Ile Val Asp Arg Ile Leu Gln Tyr 520 525 530 cgg gag ctt tcc aag ctc aag gga acc tac atc gat ccc ttg cct gcc 1743 Arg Glu Leu Ser Lys Leu Lys Gly Thr Tyr Ile Asp Pro Leu Pro Ala 535 540 545 ctg gtc cac ccc aag acg aac cgc ctc cac acc cgt ttc aac cag acg 1791 Leu Val His Pro Lys Thr Asn Arg Leu His Thr Arg Phe Asn Gln Thr 550 555 560 565 gcc acc gcc acg ggg agg ctt agc agc tcg gat ccc aac ctg caa aat 1839 Ala Thr Ala Thr Gly Arg Leu Ser Ser Ser Asp Pro Asn Leu Gln Asn 570 575 580 atc ccc gtg cgc acc cct ttg ggc cag cgg atc cgc cgg gcc ttc gtg 1887 Ile Pro Val Arg Thr Pro Leu Gly Gln Arg Ile Arg Arg Ala Phe Val 585 590 595 gcc gag gag ggg tgg agg ctg gtg gtt ttg gac tac agc cag att gag 1935 Ala Glu Glu Gly Trp Arg Leu Val Val Leu Asp Tyr Ser Gln Ile Glu 600 605 610-14 -

ctc agg gtc ctg gcg cac ctt tcc ggg gac gag aac cta atc cgg gtc 1983 Leu Arg Val Leu Ala His Leu Ser Gly Asp Glu Asn Leu Ile Arg Val 615 620 625 ttc cag gag ggc cag gac atc cac acc cag acg gcc agc tgg atg ttc 2031 Phe Gln Glu Gly Gln Asp Ile His Thr Gln Thr Ala Ser Trp Met Phe 630 635 640 645 ggc gtg ccc cca gag gcc gtg gat tcc ctg atg cgt cgg gcg gcc aag 2079 Gly Val Pro Pro Glu Ala Val Asp Ser Leu Met Arg Arg Ala Ala Lys 650 655 660 acc atc aac ttc ggc gtc ctc tac ggc atg tcc gcc cac cgg ctt tcg 2127 Thr Ile Asn Phe Gly Val Leu Tyr Gly Met Ser Ala His Arg Leu Ser 665 670 675 gga gag ctg gcc atc ccc tac gag gag gcg gtg gcc ttc atc gag cgg 2175 Gly Glu Leu Ala Ile Pro Tyr Glu Glu Ala Val Ala Phe Ile Glu Arg 680 685 690 tat ttc cag agc tac ccc aag gtg cgg gcc tgg att gag aaa acc ctg 2223 Tyr Phe Gln Ser Tyr Pro Lys Val Arg Ala Trp Ile Glu Lys Thr Leu 695 700 705 gcg gaa gga cgg gaa cgg ggc tat gtg gaa acc ctc ttt ggc cgc cgg 2271 Ala Glu Gly Arg Glu Arg Gly Tyr Val Glu Thr Leu Phe Gly Arg Arg 710 715 720 725 cgc tac gtg ccc gac ttg gct tcc cgg gtg aag agc atc cgg gag gca 2319 Arg Tyr Val Pro Asp Leu Ala Ser Arg Val Lys Ser Ile Arg Glu Ala 730 735 740 gcg gag cgc atg gcc ttc aac atg ccg gtc cag ggg acc gcc gcg gat 2367 Ala Glu Arg Met Ala Phe Asn Met Pro Val Gln Gly Thr Ala Ala Asp 745 750 755 ttg atg aaa ctg gcc atg gtg aag ctc ttt ccc agg ctt cag gag ctg 2415 Leu Met Lys Leu Ala Met Val Lys Leu Phe Pro Arg Leu Gln Glu Leu 760 765 770 ggg gcc agg atg ctt ttg cag gtg cac gac gaa ctg gtc ctc gag gct 2463 Gly Ala Arg Met Leu Leu Gln Val His Asp Glu Leu Val Leu Glu Ala 775 780 785 ccc aag gag caa gcg gag gaa gtc gcc cag gag gcc aag cgg acc atg 2511 Pro Lys Glu Gln Ala Glu Glu Val Ala Gln Glu Ala Lys Arg Thr Met 790 795 800 805 gag gag gtg tgg ccc ctg aag gtg ccc ttg gag gtg gaa gtg ggc atc 2559 Glu Glu Val Trp Pro Leu Lys Val Pro Leu Glu Val Glu Val Gly Ile 810 815 820 ggg gag gac tgg ctt tcc gcc aag gcc tag g ggtcgtgggg tatagtcgtt 2610 Gly Glu Asp Trp Leu Ser Ala Lys Ala *** 825 830 cccatgaggc gttacttcgg caccgacggg gtgcgggggg aggcgggcaa gcaccccctt 2670 acccccgggt ttgtccttaa gctt 2694 <210> 2 <211> 830 <212> PRT <213> Thermus sp. X-1 <400> 2 Met Leu Pro Leu Phe Glu Pro Lys Gly Arg Val Leu Leu Val Asp Gly - 15 -

1 5 10 15 His His Leu Ala Tyr Arg Thr Phe Phe Ala Leu Lys Gly Leu Thr Thr 20 25 30 Ser Arg Gly Glu Pro Val Gln Ala Val Tyr Gly Phe Ala Lys Ser Leu 35 40 45 Leu Lys Ala Leu Arg Glu Asp Gly Asp Val Val Ile Val Val Phe Asp 50 55 60 Ala Lys Ala Pro Ser Phe Arg His Gln Thr Tyr Glu Ala Tyr Lys Ala 65 70 75 80 Gly Arg Ala Pro Thr Pro Glu Asp Phe Pro Arg Gln Leu Ala Leu Ile 85 90 95 Lys Glu Met Val Asp Leu Leu Gly Leu Glu Arg Leu Glu Val Pro Gly 100 105 110 Phe Glu Ala Asp Asp Val Leu Ala Thr Leu Ala Lys Lys Ala Glu Lys 115 120 125 Glu Gly Tyr Glu Val Arg Ile Leu Thr Ala Asp Arg Asp Leu Tyr Gln 130 135 140 Leu Leu Ser Glu Arg Ile Ser Ile Leu His Pro Glu Gly Tyr Leu Ile 145 150 155 160 Thr Pro Glu Trp Leu Trp Glu Lys Tyr Gly Leu Lys Pro Ser Gln Trp 165 170 175 Val Asp Tyr Arg Ala Leu Ala Gly Asp Pro Ser Asp Asn Ile Pro Gly 180 185 190 Val Lys Gly Ile Gly Glu Lys Thr Ala Ala Lys Leu Ile Arg Glu Trp 195 200 205 Gly Ser Leu Glu Asn Leu Leu Lys His Leu Glu Gln Val Lys Pro Ala 210 215 220 Ser Val Arg Glu Lys Ile Leu Ser His Met Glu Asp Leu Lys Leu Ser 225 230 235 240 Leu Glu Leu Ser Arg Val Arg Thr Asp Leu Pro Leu Gln Val Asp Phe 245 250 255 Ala Arg Arg Arg Glu Pro Asp Arg Glu Gly Leu Lys Ala Phe Leu Glu 260 265 270 Arg Leu Glu Phe Gly Ser Leu Leu His Glu Phe Gly Leu Leu Glu Ser 275 280 285 Pro Val Ala Ala Glu Glu Ala Pro Trp Pro Pro Pro Glu Gly Ala Phe 290 295 300 Val Gly Tyr Val Leu Ser Arg Pro Glu Pro Met Trp Ala Glu Leu Asn 305 310 315 320 Ala Leu Ala Ala Ala Trp Glu Gly Arg Val Tyr Arg Ala Glu Asp Pro 325 330 335 Leu Glu Ala Leu Arg Gly Leu Gly Glu Val Arg Gly Leu Leu Ala Lys 340 345 350 Asp Leu Ala Val Leu Ala Leu Arg Glu Gly Ile Ala Leu Ala Pro Gly 355 360 365 Asp Asp Pro Met Leu Leu Ala Tyr Leu Leu Asp Pro Ser Asn Thr Ala 370 375 380 Pro Glu Gly Val Ala Arg Arg Tyr Gly Gly Glu Trp Thr Glu Glu Ala 385 390 395 400 Gly Glu Arg Ala Leu Leu Ser Glu Arg Leu Tyr Ala Ala Leu Leu Glu - 16 -

405 410 415 Arg Leu Lys Gly Glu Glu Arg Leu Leu Trp Leu Tyr Glu Glu Val Glu 420 425 430 Lys Pro Leu Ser Arg Val Leu Ala His Met Glu Ala Thr Gly Val Arg 435 440 445 Leu Asp Val Ala Tyr Leu Lys Ala Leu Ser Leu Glu Val Glu Ala Glu 450 455 460 Leu Arg Arg Leu Glu Glu Glu Val His Arg Leu Ala Gly His Pro Phe 465 470 475 480 Asn Leu Asn Ser Arg Asp Gln Leu Glu Arg Val Leu Phe Asp Glu Leu 485 490 495 Gly Leu Pro Ala Ile Gly Lys Thr Glu Lys Thr Gly Lys Arg Ser Thr 500 505 510 Ser Ala Ala Val Leu Glu Ala Leu Arg Glu Ala His Pro Ile Val Asp 515 520 525 Arg Ile Leu Gln Tyr Arg Glu Leu Ser Lys Leu Lys Gly Thr Tyr Ile 530 535 540 Asp Pro Leu Pro Ala Leu Val His Pro Lys Thr Asn Arg Leu His Thr 545 550 555 560 Arg Phe Asn Gln Thr Ala Thr Ala Thr Gly Arg Leu Ser Ser Ser Asp 565 570 575 Pro Asn Leu Gln Asn Ile Pro Val Arg Thr Pro Leu Gly Gln Arg Ile 580 585 590 Arg Arg Ala Phe Val Ala Glu Glu Gly Trp Arg Leu Val Val Leu Asp 595 600 605 Tyr Ser Gln Ile Glu Leu Arg Val Leu Ala His Leu Ser Gly Asp Glu 610 615 620 Asn Leu Ile Arg Val Phe Gln Glu Gly Gln Asp Ile His Thr Gln Thr 625 630 635 640 Ala Ser Trp Met Phe Gly Val Pro Pro Glu Ala Val Asp Ser Leu Met 645 650 655 Arg Arg Ala Ala Lys Thr Ile Asn Phe Gly Val Leu Tyr Gly Met Ser 660 665 670 Ala His Arg Leu Ser Gly Glu Leu Ala Ile Pro Tyr Glu Glu Ala Val 675 680 685 Ala Phe Ile Glu Arg Tyr Phe Gln Ser Tyr Pro Lys Val Arg Ala Trp 690 695 700 Ile Glu Lys Thr Leu Ala Glu Gly Arg Glu Arg Gly Tyr Val Glu Thr 705 710 715 720 Leu Phe Gly Arg Arg Arg Tyr Val Pro Asp Leu Ala Ser Arg Val Lys 725 730 735 Ser Ile Arg Glu Ala Ala Glu Arg Met Ala Phe Asn Met Pro Val Gln 740 745 750 Gly Thr Ala Ala Asp Leu Met Lys Leu Ala Met Val Lys Leu Phe Pro 755 760 765 Arg Leu Gln Glu Leu Gly Ala Arg Met Leu Leu Gln Val His Asp Glu 770 775 780 Leu Val Leu Glu Ala Pro Lys Glu Gln Ala Glu Glu Val Ala Gln Glu 785 790 795 800 Ala Lys Arg Thr Met Glu Glu Val Trp Pro Leu Lys Val Pro Leu Glu - 17 -

805 810 815 Val Glu Val Gly Ile Gly Glu Asp Trp Leu Ser Ala Lys Ala *** 820 825 830 <210> 3 <211> 830 <212> PRT <213> Thermus sp. X-1 <400> 3 Met Leu Pro Leu Phe Glu Pro Lys Gly Arg Val Leu Leu Val Asp Gly 1 5 10 15 His His Leu Ala Tyr Arg Thr Phe Phe Ala Leu Lys Gly Leu Thr Thr 20 25 30 Ser Arg Gly Glu Pro Val Gln Ala Val Tyr Gly Phe Ala Lys Ser Leu 35 40 45 Leu Lys Ala Leu Arg Glu Asp Gly Asp Val Val Ile Val Val Phe Asp 50 55 60 Ala Lys Ala Pro Ser Phe Arg His Gln Thr Tyr Glu Ala Tyr Lys Ala 65 70 75 80 Gly Arg Ala Pro Thr Pro Glu Asp Phe Pro Arg Gln Leu Ala Leu Ile 85 90 95 Lys Glu Met Val Asp Leu Leu Gly Leu Glu Arg Leu Glu Val Pro Gly 100 105 110 Phe Glu Ala Asp Asp Val Leu Ala Thr Leu Ala Lys Lys Ala Glu Lys 115 120 125 Glu Gly Tyr Glu Val Arg Ile Leu Thr Ala Asp Arg Asp Leu Tyr Gln 130 135 140 Leu Leu Ser Glu Arg Ile Ser Ile Leu His Pro Glu Gly Tyr Leu Ile 145 150 155 160 Thr Pro Glu Trp Leu Trp Glu Lys Tyr Gly Leu Lys Pro Ser Gln Trp 165 170 175 Val Asp Tyr Arg Ala Leu Ala Gly Asp Pro Ser Asp Asn Ile Pro Gly 180 185 190 Val Lys Gly Ile Gly Glu Lys Thr Ala A Lys Leu Ile Arg Glu Trp 195 200 205 Gly Ser Leu Glu Asn Leu Leu Lys His Leu Glu Gln Val Lys Pro Ala 210 215 220 Ser Val Arg Glu Lys Ile Leu Ser His Met Glu Asp Leu Lys Leu Ser 225 230 235 240 Leu Glu Leu Ser Arg Val Arg Thr Asp Leu Pro Leu Gln Val Asp Phe 245 250 255 Ala Arg Arg Arg Glu Pro Asp Arg Glu Gly Leu Lys Ala Phe Leu Glu 260 265 270 Arg Leu Glu Phe Gly Ser Leu Leu His Glu Phe Gly Leu Leu Glu Ser 275 280 285 Pro Val Ala Ala Glu Glu Ala Pro Trp Pro Pro Pro Glu Gly Ala Phe 290 295 300 Val Gly Tyr Val Leu Ser Arg Pro Glu Pro Met Trp Ala Glu Leu Asn 305 310 315 320 Ala Leu Ala Ala Ala Trp Glu Gly Arg Val Tyr Arg Ala Glu Asp Pro 325 330 335-18 -

Leu Glu Ala Leu Arg Gly Leu Gly Glu Val Arg Gly Leu Leu Ala Lys 340 345 350 Asp Leu Ala Val Leu Ala Leu Arg Glu Gly Ile Ala Leu Ala Pro Gly 355 360 365 Asp Asp Pro Met Leu Leu Ala Tyr Leu Leu Asp Pro Ser Asn Thr Ala 370 375 380 Pro Glu Gly Val Ala Arg Arg Tyr Gly Gly Glu Trp Thr Glu Glu Ala 385 390 395 400 Gly Glu Arg Ala Leu Leu Ser Glu Arg Leu Tyr Ala Ala Leu Leu Glu 405 410 415 Arg Leu Lys Gly Glu Glu Arg Leu Leu Trp Leu Tyr Glu Glu Val Glu 420 425 430 Lys Pro Leu Ser Arg Val Leu Ala His Met Glu Ala Thr Gly Val Arg 435 440 445 Leu Asp Val Ala Tyr Leu Lys Ala Leu Ser Leu Glu Val Glu Ala Glu 450 455 460 Leu Arg Arg Leu Glu Glu Glu Val His Arg Leu Ala Gly His Pro Phe 465 470 475 480 Asn Leu Asn Ser Arg Asp Gln Leu Glu Arg Val Leu Phe Asp Glu Leu 485 490 495 Gly Leu Pro Ala Ile Gly Lys Thr Glu Lys Thr Gly Lys Arg Ser Thr 500 505 510 Ser Ala Ala Val Leu Glu Ala Leu Arg Glu Ala His Pro Ile Val Asp 515 520 525 Arg Ile Leu Gln Tyr Arg Glu Leu Ser Lys Leu Lys Gly Thr Tyr Ile 530 535 540 Asp Pro Leu Pro Ala Leu Val His Pro Lys Thr Asn Arg Leu His Thr 545 550 555 560 Arg Phe Asn Gln Thr Ala Thr Ala Thr Gly Arg Leu Ser Ser Ser Asp 565 570 575 Pro Asn Leu Gln Asn Ile Pro Val Arg Thr Pro Leu Gly Gln Arg Ile 580 585 590 Arg Arg Ala Phe Val Ala Glu Glu Gly Trp Arg Leu Val Val Leu Asp 595 600 605 Tyr Ser Gln Ile Glu Leu Arg Val Leu Ala His Leu Ser Gly Asp Glu 610 615 620 Asn Leu Ile Arg Val Phe Gln Glu Gly Gln Asp Ile His Thr Gln Thr 625 630 635 640 Ala Ser Trp Met Phe Gly Val Pro Pro Glu Ala Val Asp Ser Leu Met 645 650 655 Arg Arg Ala Ala Lys Thr Ile Asn Phe Gly Val Leu Tyr Gly Met Ser 660 665 670 Ala His Arg Leu Ser Gly Glu Leu Ala Ile Pro Tyr Glu Glu Ala Val 675 680 685 Ala Phe Ile Glu Arg Tyr Phe Gln Ser Tyr Pro Lys Val Arg Ala Trp 690 695 700 Ile Glu Lys Thr Leu Ala Glu Gly Arg Glu Arg Gly Tyr Val Glu Thr 705 710 715 720 Leu Phe Gly Arg Arg Arg Tyr Val Pro Asp Leu Ala Ser Arg Val Lys 725 730 735-19 -

Ser Ile Arg Glu Ala Ala Glu Arg Met Ala Phe Asn Met Pro Val Gln 740 745 750 Gly Thr Ala Ala Asp Leu Met Lys Leu Ala Met Val Lys Leu Phe Pro 755 760 765 Arg Leu Gln Glu Leu Gly Ala Arg Met Leu Leu Gln Val His Asp Glu 770 775 780 Leu Val Leu Glu Ala Pro Lys Glu Gln Ala Glu Glu Val Ala Gln Glu 785 790 795 800 Ala Lys Arg Thr Met Glu Glu Val Trp Pro Leu Lys Val Pro Leu Glu 805 810 815 Val Glu Val Gly Ile Gly Glu Asp Trp Leu Ser Ala Lys Ala 820 825 830 <210> 4 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> X-1NE <400> 4 nnngaattca tgctgcccct ctttgagccc 30 <210> 5 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> X-1CS <400> 5 nnngtcgacc taggccttgg cggaaagcc 29-20 -