2017 Summer BIG SALE 2017.7.3 ~ 9.8 ITEM 1 P C R Enzyme cpcr Enzyme 20% High Quality Enzyme ITEM 2 cdna Synthesis cdna Synthesis Kit 최대 33 % off ITEM 3 q P C R Enzyme Two-Step / One-Step Kit NGS Library Quantification Kit Special EVENT 1 Special EVENT 2 ITEM 1, 2, 3 해당제품모두구매시 Cool Rack 2 ea 증정 Stay Cool Rack -20 보관시 3~4 시간동안상온에서 4 유지 Rack 의온도가 7 보다높아지면보라색에서핑크색으로바뀜 전품목 300 만원이상구매시 Chill Bucket + 2 L Beads Set 증정 Chill Bucket PKG 수인성오염을배제하여안전하고깨끗한실험실환경조성 + + ( 일시구매, 부가세별도 ) Chill Bucket 1 ea 2L Bead with bag Chill Packs 2ea
ITEM 1 PCR Enzyme 20% DNA Polymerase Enzyme Quick Selection Guide for Effective PCR DNA Polymerase General PCR / Colony PCR Long PCR (Max. 23 kb) Hot Start Easy to Use (with Loading dye) Hot Start Cloning Genotyping High Quality Polymerase High Fidelity PCR (80-fold higher than Taq) Long PCR (Max. 40 kb), High GC, AT Target Crude Samples Multiplex, Bisulfite Inosine Primer One-step RT-PCR Blend Taq Blend Taq Plus Quick Taq HS DyeMix -Plus- -Plus- Neo FX FX Neo -Multi & Epi- RT-PCR Quick Master Mix 범용적으로다양한 PCR 에사용 Blend Taq Series 일반 DNA polymerase 에 proofreading 추가로효과적인 PCR 가능 Taq polymerase보다 3-4배낮은 error rate 최대 23 kb까지증폭 Blend Taq M : 1 kb Ladder Marker 1 : β-globin 1.3 kb 2 : β-globin 2.7 kb Blend Taq -Plus- 3 : β-globin 3.6 kb 4 : β-globin 17.5 kb 5 : β-globin 23.0 kb High efficient Taq DNA polymerase (Company A) M 1 2 3 4 5 M M 1 2 3 4 5 M M 1 2 3 4 5 M Sample : Human genomic DNA Target : β-globin gene Cycling condition : 94, 2 min. 94, 30 sec. 60, 30 sec. 72, 1 min./kb 30 cycles 2x Master Mix 형태로 PCR 과 Gel Loading 까지한번에 Quick Taq HS DyeMix Hot-start PCR로특이적인반응가능높은증폭효율로 colony-direct PCR 가능최대 4 kb 까지증폭 One tube 에서 Reverse Transcription 과 PCR 을한번에 RT-PCR Quick Master Mix 2X master mix로사용자편의성제공 RNA 2차구조형성및 GC-rich 타겟도높은효율로증폭가능 Hot-start PCR을통한높은효율과정확성낮은 copy에서도능폭가능한높은 sensitivity Company A RT-PCR Quick Master Mix 10 6 10 5 10 4 10 3 B 10 6 10 5 10 4 10 3 10 2 B In vitro translation 결과 template human G3PDH gene 450 bp 품번품명 * Hot-Start PCR을위한 anti-taq DNA polymerase가포함되어있음규격행사가 (VAT 별도 ) TOBTQ-101 TOBTQ-201 TODTM-101 TOPCR-311 Blend Taq 2.5 U/ul Blend Taq, 10x buffer, 2 mm dntps 200 rxn Blend Taq -Plus- 2.5 U/ul Blend Taq -Plus-*, 10x buffer, 2 mm dntps Quick Taq HS DyeMix 2x Quick Taq HS DyeMix* RT-PCR Quick Master Mix 2x RT-PCR Quick Master Mix, 50 mm Mn(OAc) 2, Nuclease-free water Positive control RNA (G3PDH mrna), Control primer F & R 100 rxn 50 rxn 20% 102,400 112,500 88,000 302,400
High Quality Polymerase Enzyme Thermal Stability Extension Rate Processivity Fidelity 최대 7.5 배높음약 5 배빠름 15 배이상높음최대 80 배높음 Taq DNA Polymerase 보다 80 배높은 PCR Fidelity Property DNA polymerase Value for indicated DNA polymerase U DNA polymerase Pfu DNA polymerase Taq DNA polymerase -Plus- Neo -Plus- FX Pfu DNA Polymerase Long PCR enzyme (A company) Taq DNA Polymerase 0.0 25.0 50.0 75.0 100.0 125.0 150.0 Mutation frequency(number of misincorporated bases/100,000 bases) Origin Archaea Archaea Archaea Bacteria Thermostability (half-life) 95, 12h; 100, 3.0h 95, 12h; 100, 3.0h 95, 6h; 100, 2.9h 95, 1.6h 5' 3' exonuclease activity - - - + 3' 5' exonuclease activity + + + - Processivity (bases) > 300 > 300 < 20 ND Elongation rate (bases/sec) 106 ~138 106 ~138 25 61 Use of DNA containig uracil - + - + Multiplexing 및 Bisulfite PCR 에특화된제품 -Multi & Epi- Multiplexing Multi-target 에대한균일한증폭효율 Multiplex PCR 에최적화 (10 개의타겟, 최대 10 kb 까지증폭가능 ) Uracil(U) 포함 DNA 도인식가능한 U enzyme 사용 단일유전자증폭시최대 40 kb 까지증폭가능 Cloning 용 High Fidelity Enzyme -Plus- Series 일반 Taq polymerase 보다 80 배높은정확도 Buffer 조건최적화로 PCR 증폭길이, 증폭효율향상 최대 24 kb 까지증폭 NGS Library 합성에효과적 Epigenetic / Metagenomic 실험에효과적 Epigenetic Uracil(U) 포함 target 증폭 Metagenomic Uracil(U), Inosine(I) 포함 primer 증폭 Colony Total No. Colony Mutant No. Mutation frequency (%) -Plus- 10,610 10 0.09 High fidelity PCR enzyme ( 타사 ) 10,900 68 0.62 Pfu based DNA polymerase 5,520 76 1.17 Taq DNA polymerase 10,560 780 7.39 Crude 샘플및 High GC 타겟에최적화 FX Series gdna 추출없이 direct PCR 가능최대 40 kb까지증폭가능 Directly 품번품명 * Hot-Start PCR을위한 anti-taq DNA polymerase가포함되어있음규격정상가 (VAT 별도 ) TOKME-101 TO-201 TO-401 TOKFX-101 TOKFX-201 -Multi & Epi- High Quality -Multi & Epi- (1.0 U/ul)*, 2x PCR buffer for -Multi & Epi- -Plus- -Plus- (1.0 U/ul)*, 10x PCR buffer for -Plus-, 25 mm MgSO 4, 2 mm dntps -Plus- Neo BEST -Plus- Neo (1.0 U/ul)*, 10x PCR buffer for -Plus- Neo 25 mm MgSO 4, 2 mm dntps FX BEST FX (1.0 U/ul)*, 10x PCR buffer for FX, 2 mm dntps FX Neo FX Neo (1.0 U/ul)*, 10x PCR buffer for FX Neo, 2 mm dntps 200 rxn 305,400 202,400 270,000
ITEM 2 cdna Synthesis ReverTra Ace cdna Synthesis Kit ReverTra Ace d M-MLV RTase 의 RNase H domain 부위에유전자조작을시켜, Rnase H activity 를낮춤으로써 mrna 의 degradation 이방지되어 cdna 합성효율이증가함 cdna Synthesis kit Selection Guide 특성 ReverTra Ace -α- Oligo (dt) 20 & Random primer vial each ReverTra Ace qpcr RT kit Oligo (dt) 20 & Random primer Mix type ReverTra Ace qpcr RT Master Mix Master mix type ReverTra Ace qpcr RT Master Mix with gdna remover Master mix with gdna remover SuperPrep Cell Lysis & RT kit for qpcr Cell lysis & RT Master mix (No need RNA purification) 합성시간 25 min 15 min 30 min 35 min 합성 cdna Long cdna ( 14kb) short-chain cdna (< 5kb) 응용 for RT PCR Library prep Gene expression for qpcr Gene expression RNA Quantification input RNA 1 pg ~ 1 μg 1 pg ~ 1 μg 0.5 pg ~ 0.5μg 1 10 1 ~7 10 4 cells Long cdna 합성이가능한 High Quality RT Kit ReverTra Ace -α- 14 kb 이상의 Long cdna 합성가능 Library 제작및 RT-PCR 에사용 Genomic DNA removal (Degradation) RNA Sample 4x DNA Master Mix with gdna Remover 37, 5 min Total RNA 에포함된 gdna 제거와 cdna 합성을하나의 kit 로빠르고간편하게진행 ReverTra Ace qpcr RT Master Mix with gdna Remover gdna 오염에의한 qpcr 결과의오류방지 타사대비월등히높은 gdna 제거율과 30 분이내의빠르고간편한실험 Directly cdna Synthesis cdna (gdna-free) Real-time PCR etc. 5x RT Master Mix Ⅱ 37,15 min 98, 5 min TOFSK-101 TOFSQ-301 ReverTra Ace -a- kit ReverTra Ace 5x RT buffer (contains 25 mm Mg 2+ ), RNase inhibitor (10 U/ul) dntps mixture (10 U/ul), RNase-free water, Olido (dt)20 (10 pmol/ul) Random primers (25 pmol/ul), Control primer F, R (10 pmol/ul) Positive control RNA (10 5 copies/ul) ReverTra Ace qpcr RT Master Mix with gdna Remover BEST gdna remover, 4x DN Master mix, 5x RT Master mix II, 5x RT Master mix II no RT-control Nuclease-free water 100 rxn 200 rxn 400,000
15 분만에반응완료되는고효율 RT Kit ReverTra Ace qpcr RT kit 적절한시약구성으로타사대비높은합성효율 Rnase H를처리하는과정이없어합성시간단축 1 개의 Master Mix 타입으로간편하게 cdna 합성 ReverTra Ace qpcr RT Master Mix 간편한 protocol과시약구성으로사용편의성증대 15분만에 cdna 합성완료 TOFSQ-101 TOFSQ-201 ReverTra Ace qpcr RT kit 5x RT buffer, Enzyme Mix (RTase, RNase inhibitor), Primer Mix (oligo dt, random primer) Nuclease-free water ReverTra Ace qpcr RT Master Mix BEST 5x RT Master mix, 5x RT Master mix no RT-control, Nuclease-free water 200 rxn 350,000 360,000 Cell Lysis & DNaseⅠTreatment 7 min Total RNA 추출없이 cdna 합성을약 35 분만에완료 SuperPrep Cell Lysis & RT kit for qpcr Cultured cell 에서 High throughput RNA level screening 실험에추천실험단계및시간단축으로 RNA degradation 방지다양한 cell line 에서검증완료 Directly Reverse Transcription (RT) 25 min 15 개 house keeping gene 의 purified RNA 와 qpcr 효율비교 (2.5 X 10 4 HEK293 cells) TOSCQ-101 SuperPrep Cell Lysis & RT kit for qpcr Lysis Reagents (Lysis solution, gdna remover, Stop solution, RNase inhibitor) RT Reagents 5x RT Mater mix, 5x RT Master mix no RTcontrol, Nuclease-free water 100 rxn 1,142,100
ITEM 3 qpcr Enzyme Real Time PCR Master Mix Selection Guide Product Realtime PCR Series High efficiency Two-Step qpcr Master Mix Thunderbird Series High Specificity, sensitivity SYBR qpcr mix Best Stability on Difficult condition One-Step qpcr Master mix Thunderbird Probe one-step qrt-pcr mix Best Performance on multiplex Long qpcr < 1 kb < 2 kb < 1 kb RNA-direct TM Realtime PCR Series High efficiency GC-rich targets + + +++ ( 83%) ++ ++ Crude samples + + qpcr instrument +++ Whole blood, animal & plant tissue All qpcr - - Fluorescence type SYBR Green / Probe SYBR Green Probe SYBR Green / Probe Real Time PCR Master Mix - Two Step Kit 까다로운샘플및 Target gene 을위한 High-Quality qpcr Mix SYBR qpcr Mix qpcr에서도최대 2 kb target 확인우수한내열성으로 GC-rich ( 최대 83%) target 에서도높은증폭효율보장쥐꼬리및혈액등의샘플에서 gdna 추출없이 direct qpcr 가능 SYBR qpcr Mix Conventional master mix (Taq based) Amplification curve 10 4 10 1 copy 10 4 10 1 copy Directly Melting curve 범용적인 qpcr Master Mix 의 Best Seller Realtime PCR Series 탁월한반응특이성으로 primer dimer 형성억제사용이편리한 master mix 타입 (ROX reference dye 포함 ) 정확성과증폭효율을모두겸비한 qpcr Master Mix THUNDERBIRD Series 특허출원 PCR enhancer로증폭효율및 dynamic range 증가최적화된 buffer 조성으로높은검출민감도보장모든 qpcr 장비에호환가능한제품구성 50x ROX reference dye 개별 vial로구성
또는 2 + ReverTra Ace qpcr RT Master Mix 중택 1 TOQKD-201 SYBR qpcr Mix High Quality 2x SYBR qpcr Mix, 50x ROX reference dye 3 x 1.67 ml 250 ul 349,000 TOQPK-101 TOQPK-201 TOQPS-101 Real-time PCR Master Mix 2x Real-time PCR Master Mix SYBR Green Real-time PCR Master Mix 2x SYBR Green Real-time PCR Master Mix BEST THUNDERBIRD qpcr Probe Mix 2x THUNDERBIRD qpcr Probe Mix, 50x ROX reference dye 또는 2 + ReverTra Ace qpcr RT Master Mix 중택 1 5 x 1 ml 290,000 3 x 1.67 ml 250 ul 280,000 TOQPS-102 THUNDERBIRD qpcr SYBR Mix BEST 2x THUNDERBIRD qpcr SYBR Mix, 50x ROX reference dye 3 x 1.67 ml 250 ul 280,000 Real Time PCR Master Mix - One Step Kit 우수한민감도와 Multiplex 에특화된제품 THUNDERBIRD Probe One-Step qrt-pcr Kit 높은증폭효율로 10 copies 수준의 RNA virus 검출가능 PCR bias 를낮춰다양한 target을고르게증폭하여 multiplexing qrt-pcr 에효과적 dutp를포함하여 UNG 처리를통한위양성검출위험없음 최적화된 Buffer 조성을통해증폭효율강화 RNA-direct TM Realtime PCR Series Tth polymerase 로구성되어 cdna 합성과 DNA 증폭동시진행가능간소화한실험을통해샘플오염방지및재현성증가 TOQRZ-101 TOQRT-101 TOQRT-201 THUNDERBIRD Probe One-Step qrt-pcr kit High Quality 2x Reaction buffer (contains dutp), DNA polymerase, RT Enzyme mix, 50x ROX reference dye, RNase free water RNA-direct TM Realtime PCR Master Mix 2x RNA-direct Realtime PCR Master Mix, 50 nm Mn(OAc) 2, Rnase free water RNA-direct TM SYBR Green Realtime PCR Master Mix 2x RNA-direct SYBR Green Real-time PCR Master Mix, 50 nm Mn(OAc) 2, Rnase free water 100 rxns 377,300 405,500
ITEM 3 qpcr Enzyme GenNext TM NGS Library Quantification Kit Illumina Platform 의 NGS Library qpcr Quantification Kit polymerase 사용으로기존 Taq-based 제품보다우수한증폭효율과높은정확도 6 개의 standard DNA (20 ~ 0.0002 pm, 452 bp) 로정확한 standard curve 보장 (R 2 값 0.99 이상 ) Dilution buffer 까지 all-in-one 으로구성하여고객편의성제공 모든 qpcr 장비와호환가능한구성 (50x ROX reference dye 개별 vial 로구성 ) High GC-/AT- target 에서도 95% 이상의높은증폭효율보장 다양한종류와 size 의 library 에대해서도 95% 이상의안정적인증폭효율보장 GenNext TM NGS Library Quantification Kit High Quality TONLQ-101 SYBR qpcr Mix Set SYBR qpcr Mix 50x ROX reference dye Standard & Primer Set Standard DNA 1~6 (20 ~ 0.0002 pm, 200 ul each) 5x Primer Mix 50x Dilution Buffer 500 rxns 20 ul 반응기준 834,100 제품주문 A/S 문의 02-3471-6500 042-824-7000 info@bmskorea.co.kr 02-3471-8171 service@bmskorea.co.kr Copyrightc 2017 Bio-Medical Science Co., Ltd. All rights reserved. 017BM06C