2016 TOYOBO SUMMER SALE 2016.7.4 ~ 9.9 cdna Synthesis Kit qpcr Master Mix KOD Polymerase Enzyme DNA Polymerase Enzyme Immuno Enhancer
ReverTra Ace cdna Synthesis Kit d ReverTra Ace M-MLV RTase의 RNase H domain 부위에유전자조작을시켜, Rnase H activity를낮추어 mrna의 degradation을방지하여 cdna 합성효율을높임 Long cdna 합성이가능한 High Quality RT Kit ReverTra Ace -a- RT 반응에필요한모든시약구성 효소와버퍼조성을최적화하여 14 kb 이상의 cdna 합성가능 Library 구축및 RT-PCR에사용 품번 품명 구성 규격 정상가 (VAT 별도 ) TOFSK-101 ReverTra Ace -a- - ReverTra Ace - 5x RT buffer (contains 25 mm Mg 2+ ) - RNase inhibitor (10 U/ul) - dntps mixture (10 U/ul) - RNase-free water - Olido (dt) 20 (10 pmol/ul) - Random primers (25 pmol/ul) - Control primer F (10 pmol/ul) - Control primer R (10 pmol/ul) - Positive control RNA (10 5 copies/ul) 100 rxn 400,000 Total RNA 에포함된 gdna 제거와 cdna 합성을하나의 Kit 로빠르고간편하게진행 ReverTra Ace qpcr RT Master Mix with gdna Remover 오염된 gdna로인한 qpcr 결과의오류제거 타사대비월등히높은 gdna 제거율과빠르고간편한실험 Genomic DNA removal (Degradation) cdna Synthesis RNA Sample 4x DNA Master Mix (with gdna Remover) 37 5min Directly cdna (gdna-free) Real-time PCR etc 5x RT Master Mix Ⅱ 37 15min / 98 5min TOFSQ-301 ReverTra Ace qpcr RT Master Mix with gdna Remover - gdna remover - 4x DN master mix - 5x RT master mix II - 5x RT master mix II no RT-control - Nuclease-free water s 400,000 주문및상담 02-3471-6500 l 042-824-7000 l www.bmskorea.co.kr
15 분만에 RT 반응이완료되는고효율 RT Kit ReverTra Ace qpcr RT Kit 적절한시약구성으로타사대비높은합성효율 RNase H 를처리하는과정이없어실험시간단축 1 개의 Master Mix 타입으로편리하게 cdna 합성 ReverTra Ace qpcr RT Master Mix 간편한 protocol과시약구성으로사용편의성증대 15분만에 cdna 합성완료 TOFSQ-101 ReverTra Ace qpcr RT kit - 5x RT buffer - Enzyme mix (RTase, RNase inhibitor) - Primer mix (oligo dt, random primer) - Nuclease-free water s 350,000 TOFSQ-201 ReverTra Ace qpcr RT Master Mix - 5x RT master mix - 5x RT master mix no RT-control - Nuclease-free water s 360,000 Total RNA 추출없이 cdna 합성을약 30 분만에완료 SuperPrep Cell Lysis & RT Kit for qpcr 실험단계및시간단축으로 RNA degradation 방지 High-throughput 실험에최적화 다양한 cell line에서테스트완료 Cell Lysis & DNaseⅠ Treatment Directly! 7분! Reverse Transcription (RT) 25 분! Real-time PCR 1-step Real-time PCR (simplifed assay) TOSCQ-101 SuperPrep Cell Lysis & RT kit for qpcr - Lysis reagents (Lysis solution, 5x RT mater mix, 5x RT mater mix no RT-control, nuclease-free water) - RT reagents (gdna remover, stop solution, RNase inhibitor) 100 rxn 685,200
Two-Step qpcr Master Mix 모든샘플및 Target Gene 을확인할수있는초강력 qpcr Mix KOD SYBR qpcr Mix KOD SYBR qpcr Mix Amplification curve Conventional master mix (Taq based) qpcr에서도최대 2 kb target 확인 우수한내열성으로 GC-rich ( 최대 83%) target에서도높은증폭효율보장 쥐꼬리및혈액등의샘플에서 gdna 추출과정없이 direct qpcr 가능 Melting curve 10 4 10 1 copy 10 4 10 1 copy - 2x KOD SYBR qpcr Mix 3 x 1.67 ml TOKME-101 KOD SYBR qpcr Mix 209,400-50x ROX reference dye 250 ul 범용적인 qpcr Master Mix 의 Best Seller Realtime PCR series 탁월한반응특이성으로 primer dimer가생기지않음 사용이편리한 master mix 타입 (ROX reference dye 포함 ) TOQPK-101 Real-time PCR Master Mix - 2x Real-time PCR master mix 5 x 1 ml 290,000 TOQPK-201 SYBR Green Real-time PCR Master Mix - 2x SYBR green Real-time PCR master mix 5 x 1 ml 290,000 정확성과증폭효율을모두겸비한 qpcr Master THUNDERBIRD Series 특허출원 PCR enhancer로증폭효율및 dynamic range 증가 최적화된 buffer 조성으로높은검출민감도보장 모든 qpcr 장비에호환가능한제품구성 50x ROX reference dye가개별 vial로구성 TOQPS-101 TOQPS-201 THUNDERBIRD Probe qpcr Mix THUNDERBIRD SYBR qpcr Mix - 2x THUNDERBIRD Probe qpcr mix - 50x ROX reference dye - 2x THUNDERBIRD SYBR qpcr mix - 50x ROX reference dye 3 x 1.67 ml 250 ul 3 x 1.67 ml 250 ul 280,000 280,000 주문및상담 02-3471-6500 l 042-824-7000 l www.bmskorea.co.kr
One-Step qpcr Master Mix Tth Polymerase 와특화된 RT Enzyme 으로민감도극대화 THUNDERBIRD Probe One-Step qrt-pcr Kit 높은증폭효율로 10 copies 수준의 RNA virus도검출 PCR bias를낮춰다양한 target 서열을고르게증폭하여 multiplexing qrt-pcr에효과적 dutp를포함하고있어 UNG 처리를통한위양성검출위험없음 PCR inhibitor에대한높은저항성 TOQRZ-101 THUNDERBIRD Probe One-Step qrt-pcr kit - 2x Reaction buffer (contains dutp) - DNA polymerase - RT Enzyme mix - 50x ROX reference dye - RNase free water 100 rxns 226,300 최적화된 Buffer 조성을통해증폭효율강화 RNA-direct TM Realtime PCR Series Tth polymerase로구성되어 cdna 합성과 DNA 증폭동시진행가능 간소화한실험을통해샘플오염방지및재현성증가 TOQRT-101 TOQRT-201 RNA-direct TM Realtime PCR Master Mix RNA-direct TM SYBR Green Realtime PCR Master Mix - 2x RNA-direct Real-time PCR master mix - 50 nm Mn(OAc) 2 - RNase free water - 2x RNA-direct SYBR green Real-time PCR master mix - 50 nm Mn(OAc) 2 - RNase free water 100 rxns 100 rxns 405,500 405,500
High Quality KOD DNA Polymerase Enzyme 최대 7.5 배높음 약 5 배빠름 15 배이상높음 최대 80 배높음 Thermal Stability 온도안정성 Extension Rate 신장속도 Processivity 반응의연속성 Fidelity 정확도 Property Value for indicated DNA polymerase KOD DNA polymerase UKOD DNA polymerase Pfu DNA polymerase Taq DNA polymerase Taq DNA Polymerase 보다 80 배높은 PCR Fidelity Origin Archaea Archaea Archaea Bacteria Thermostability (half-life) 95, 12h; 100, 3.0h 95, 12h; 100, 3.0h 95, 6h; 100, 2.9h 95, 1.6h 5' 3' exonuclease activity - - - + 3' 5' exonuclease activity + + + - Processivity (bases) > 300 > 300 < 20 ND Elongation rate (bases/sec) 106 ~138 106 ~138 25 61 Use of DNA containig uracil - + - + KOD -Plus- Neo KOD -Plus- KOD FX Pfu DNA Polymerase Long PCR enzyme (A company) Taq DNA Polymerase 0.0 25.0 50.0 75.0 100.0 125.0 150.0 Mutation frequency(number of misincorporated bases/100,000 bases) Multiplexing 및 Bisulfite PCR 에특화된제품 KOD -Multi & Epi- PCR bias를최소화하여 multiplexing에최적화 (10 kb까지가능 ) 업그레이드된 UKOD로구성되어 Epigenetic 및 Metagenomic 실험에도효과적인 PCR 반응결과 최대 40 kb까지증폭 - KOD -Multi & Epi- (1.0 U/ul) * TOKME-101 KOD -Multi & Epi- 183,200-2x PCR buffer for -Multi & Epi- * Hot-Start PCR을위해 anti-kod DNA polymerase 포함 PCR Inhibitor 가다량포함된샘플및 GC-rich 타겟에최적 KOD FX Series gdna 추출없이 direct-pcr 가능 일반 Taq polymerase 보다 11배높은정확도 최대 40 kb까지증폭 높은증폭효율과정확도를겸비하여 GC-rich 샘플의 cloning 및 genotying 용으로최적 TOKFX-101 TOKFX-201 KOD FX KOD FX Neo - KOD FX (1.0 U/ul) * - 10x PCR buffer for KOD FX - 2 mm dntps - KOD FX Neo (1.0U/ul) * - 10x PCR buffer for KOD FX Neo - 2 mm dntps 270,000 270,000 * Hot-Start PCR 을위해 anti-kod DNA polymerase 포함 주문및상담 02-3471-6500 l 042-824-7000 l www.bmskorea.co.kr
일본 Kodakara 섬온천지역에서서식하는 Thermococcus kodakaraensis KOD1 에서유래한 TOYOBO 사만의특별한효소, KOD DNA polymerase 높은정확도가필요한 Cloning 용 High Fidelity Enzyme KOD -Plus- Series 일반 Taq polymerase보다 80배높은정확도 PCR반응 buffer의최적화로증폭길이, 증폭효율향상 최대 24 kb까지증폭 Colony number Mutation Total Muntant frequency (%) KOD -Plus- 10,610 10 0.09 High fidelity PCR enzyme ( 타사 ) 10,900 68 062 Pfu based DNA polymerase 5,520 76 1.17 Taq DNA polymerase 10,560 780 0.09 TOKOD-201 TOKOD-401 KOD -Plus- KOD -Plus- Neo - KOD -Plus- (1.0 U/ul) * - 10x PCR buffer for KOD -Plus- - 25 mm MgSO4-2 mm dntps - KOD -Plus- Neo (1.0 U/ul) * - 10x PCR buffer for KOD -Plus- Neo - 25 mm MgSO 4-2 mm dntps 202,400 202,400 * Hot-Start PCR 을위해 anti-kod DNA polymerase 포함 범용적으로다양한 PCR 에사용 Blend Taq Series 일반 DNA polymerase와 proofreading 기능의효소가섞인제품 Taq polymerase보다 3 ~ 4배낮은 error rate 최대 23 Kb 까지증폭 TOBTQ-101 TOBTQ-201 Blend Taq Blend Taq -Plus- - Blend Taq (2.5 U/ul) - 10x buffer - 2mM dntps - Blend Taq -Plus- (2.5U/ul) * - 10x buffer - 2mM dntps 96,000 105,500 * Hot-Start PCR 을위해 anti-taq DNA polymerase 포함 2x Master Mix 형태로 PCR 과 Gel Loading 까지한번에 Quick Taq HS DyeMix Hot-start PCR로특이적인반응가능 높은증폭효율로 colony-direct PCR 가능 최대 4 Kb까지증폭 Quick Taq HS DyeMix* Primers PCR-grade water 100 rxn TODTM-101 Quick Taq HS DyeMix - 2x Quick Taq HS DyeMix * 82,500 * Hot-Start PCR 을위해 anti-taq DNA polymerase 포함
Immuno Enhancer Western Blot, Dot Blot 및 ELISA 실험에적용 Can Get Signal Signal-to-noise 가개선되어 target signal 증폭효과 최적의조건에서항원 - 항체가반응하여특이성증가 Signal 은높게, Background 는낮게도출된결과 Sample : Cultured bovine adrenal medulla cells 1. Control (H 2O) 2. Insulin (1 nm, stimulated for 5 min) 3. Insulin (10 nm, stimulated for 5 min) 4. Insulin (100 nm, stimulated for 5 min) 조직및세포에서 IHC 실험에적용 Can Get Signal Immunostain Signal의민감도와정확도를높여선명한이미지구현 샘플에따라적합한제품을선택하여사용 (A or B solution) <Specimen> Human brain cancer (glioblastoma) paraffin embedded section <Specimen> Drosophila culture cell line (schneider 2 (s2) cells) Conventional (1% BSA/PBSΧ 䠻 Can Get Signal immunostain (Solution A) Can Get Signal 䠻 immunostain (Solution B) (1) Conventional (1% BSA, 0.02% TX-100 /PBSΧ 䠻 (2) Can Get Signal immunostain (Solution A) 䠻 (3) Can Get Signal immunostain (Solution B) TONKB-101 Can Get Signal Solution 1 / Solution 2 각 250 ml 271,100 TONKB-401 Can Get Signal Immunostain Solution A / Solution B 각 5 ml 98,100 서울시강남구역삼로 7 길 22 BMS 빌딩 Tel. 02-3471-6500 ㅣ Fax. 02-3472-1211 대전시유성구계룡로 141 번길 30-12 Tel. 042-824-7000 ㅣ Fax. 042-828-6446 본자료는친환경재생용지와콩기름잉크로제작되었습니다. ( 주 ) 비엠에스는본사에서판매한제품에한해서비스를보장합니다. Copyrightc 2015 BMS Co., Ltd. All rights reserved. 015BM06B