2018 TOYOBO BIG SALE 기간 : 2018년 7월 16일 ~ 9월 21일까지 TOYOBO 전제품 최대 33% SALE PCR Enzyme cpcr Enzyme cdna Synthesis cdna Synthesis Kit qpcr Enzyme One-Step / Two-Step Kit Immuno Enhancer High Quality Enzyme NGS Library Quantification Kit 전제품 300만원 이상 구매 시 전제품 500만원 이상 구매 시 ARA Mini Magnetic 증정 Stirrer 증정 i-myrun II Electrophoresis System 증정 Speed Range 100 ~ 1,500 RPM 일시 구매 시 / 부가세 별도 증정 일시 구매 시 / 부가세 별도
DNA Polymerase Enzyme Quick Selection Guide for Effective PCR DNA Polymerase High Quality Polymerase General PCR / Colony PCR High Fidelity PCR (80-fold higher than Taq) Long PCR (Max. 23 kb) Long PCR (Max. 40 kb), High GC, AT Target One-step RT-PCR Hot Start Easy to Use (with Loading dye) Hot Start T-vec Cloning Cloning (Blunt end)* Genotyping Crude Samples Multiplex, Bisulfite Inosine Primer Blend Taq Blend Taq Plus Quick Taq HS DyeMix Dash -Plus- -Plus- Neo FX FX Neo -Multi & Epi- RT-PCR Quick Master Mix * T-vec cloning 시, A-attachment mix 사용필요 범용적으로다양한 PCR 에사용 Blend Taq Series Proofreading 기능을더해효과적인 PCR 가능한 rtaq polymerase 제품일반 Taq polymerase 보다 3 ~ 4배낮은 error rate 최대 23 kb까지증폭 Blend Taq M 1 2 3 4 5 M M 1 2 3 4 5 M M 1 2 3 4 5 M M : 1 kb Ladder Marker 1 : β-globin 1.3 kb 2 : β-globin 2.7 kb Blend Taq -Plus- 3 : β-globin 3.6 kb 4 : β-globin 17.5 kb 5 : β-globin 23.0 kb High efficient Taq DNA polymerase (Company A) Sample : Human genomic DNA Target : β-globin gene Cycling condition : 94, 2 min. 94, 30 sec. 60, 30 sec. 72, 1 min./kb 30 cycles TOBTQ-101 Blend Taq Blend Taq (2.5 U/ul) 10x buffer 2 mm dntps 200 rxn 102,400 TOBTQ-201 Blend Taq -Plus- Blend Taq -Plus- (2.5 U/ul) * 10x buffer 2 mm dntps (50 ul 반응기준 ) 112,500 2X Master Mix 형태로 PCR 과 Gel Loading 까지한번에! Quick Taq HS DyeMix Hot-start PCR 로비특이적반응방지 높은증폭효율로 colony-direct PCR 가능 최대 4 kb 까지증폭 * Hot-Start PCR 을위해 anti-taq DNA pol. antibodies 포함 TODTM-101 Quick Taq HS DyeMix 2x Quick Taq HS DyeMix * 100 rxn (50 ul 반응기준 ) 90,300 * Hot-Start PCR 을위해 anti-taq DNA pol. antibodies 포함 One Tube 에서 Reverse Transcription 과 PCR 을한번에! RT-PCR Quick Master Mix 2x master mix로사용자편의성제공 RNA의 2차구조형성및 GC-rich 타겟도높은효율로증폭가능 Hot-start PCR을통한높은효율과정확성낮은 copy에서도증폭가능한높은 sensitivity Company A RT-PCR Quick Master Mix 10 6 10 5 10 4 10 3 B 10 6 10 5 10 4 10 3 10 2 B In vitro translation 결과 template human G3PDH gene 450 bp TOPCR-311 RT-PCR Quick Master Mix 2x RT-PCR Quick Master Mix, 50 mm Mn(OAc)2, Nuclease-free water, Positive control RNA (G3PDH mrna), Control primer F & R 50 rxn (50ul 반응기준 ) 302,400 TOTAK-301 10X A-attachment Mix 10 x A-attachment mix 25 rxn 78,000 TOLGK-201 Ligation high ver.2 Ligation high ver.2 750 ul 158,000
High Quality Polymerase Enzyme Thermal Stability 온도안정성 최대 7.5 배높음 Extension Rate 신장속도 약 5 배빠름 Processivity 반응의연속성 15 배이상높음 Fidelity 정확도 최대 80 배높음 Taq DNA Polymerase 보다 80 배높은 PCR Fidelity -Plus- Neo -Plus- FX Pfu DNA Polymerase Long PCR enzyme (A company) Taq DNA Polymerase 0.0 25.0 50.0 75.0 100.0 125.0 150.0 Mutation frequency(number of misincorporated bases/100,000 bases) Property DNA polymerase Value for indicated DNA polymerase U DNA polymerase Pfu DNA polymerase Taq DNA polymerase Origin Archaea Archaea Archaea Bacteria Thermostability (half-life) 95, 12h; 100, 3.0h 95, 12h; 100, 3.0h 95, 6h; 100, 2.9h 95, 1.6h 5' 3' exonuclease activity - - - + 3' 5' exonuclease activity + + + - Processivity (bases) > 300 > 300 < 20 ND Elongation rate (bases/sec) 106 ~138 106 ~138 25 61 Use of DNA containig uracil - + - + T-vec Cloning 과 Blunt end Cloning 을자유롭게! Dash Taq 대비 2배빠른연장속도 Taq polymerase 보다 3배낮은 error rate A-overhang end와 blunt end product를동시에합성 1 2 3 4 1 2 3 4 M M 1 2 3 4 1 2 3 4 Template: HCV cdna synthesized from HCV RNA isolated from serum specimens Target: HCV pre-core gene TOLDP-101 Dash Dash (2.5 U/ul), 10x PCR Buffer for Dash 2 mm dntps 200 rxn (50 ul 반응기준 ) 308,000 Multiplexing 및 Bisulfite PCR 에특화된제품 -Multi & Epi- 단일유전자증폭시, 최대 40 kb까지증폭가능 NGS Library 합성에효과적 : Multiplex PCR에최적화 (10개의타겟유전자, 최대 10 kb까지증폭가능 ) Epigenetic 및 Metagenomic 실험에효과적 : Uracil(U) 포함 DNA도인식가능한 U enzyme 사용 -Multi & Epi- (1.0 U/ul) * TOKME-101 -Multi & Epi- 2x PCR buffer for -Multi & Epi- 200 rxn (50 ul 반응기준 ) 305,400 * Anti- DNA pol. antibodies 포함 Crude 샘플및 High GC 타겟에최적화 FX Series gdna 추출없이 direct PCR 가능최대 40 kb까지증폭가능 품번 품명 구성 규격 행사가 (VAT 별도 ) TOKFX-101 FX FX (1.0 U/ul) * 10x PCR buffer for FX, 2 mm dntps 200 rxn 270,000 TOKFX-201 FX Neo FX Neo (1.0 U/ul) * 10x PCR buffer for FX Neo, 2 mm dntps (50 ul 반응기준 ) 270,000 * Anti- DNA pol. antibodies 포함 Cloning 용 High Fidelity Enzyme -Plus- Series 일반 Taq polymerase 보다 80 배높은정확도 Buffer 조건최적화로 PCR 증폭길이와효율향상 최대 24 kb 까지증폭 TO-201 TO-401 -Plus- -Plus- Neo TOSMK-101 -Plus- Mutagenesis Kit -Plus- (1.0 U/ul) *, 10x PCR buffer for -Plus-, 25 mm MgSO4, 2 mm dntps 202,400 -Plus- Neo (1.0U/ul) *, 10x PCR buffer for 200 rxn -Plus- Neo, 25 mm MgSO4, 2 mm dntps (50 ul 반응기준 ) 202,400 -Plus- (1 U/ul), 1x Buffer, 2 mm dntps, DpnⅠ(10 U/ul), Ligation high, control plasmid pak119m, control primer #1, 2 248,400 * Anti- DNA pol. antibodies 포함
ReverTra Ace cdna Synthesis kit d ReverTra Ace M-MLV RTase의 RNase H domain 부위에유전자조작을시켜, RNase H activity를낮추어 mrna의 degradation을방지하여 cdna 합성효율을높임 cdna Synthesis kit Selection Guide 특성 ReverTra Ace -α- Oligo (dt) 20 & Random primer vial each ReverTra Ace qpcr RT kit Oligo (dt) 20 & Random primer Mix type ReverTra Ace qpcr RT Master Mix Master mix type ReverTra Ace qpcr RT Master Mix with gdna remover Master mix with gdna remover SuperPrep Cell Lysis & RT kit for qpcr Cell lysis & RT Master mix (No need RNA purification) 합성시간 25 min 15 min 30 min 30 min 합성 cdna Long cdna ( 14kb) short-chain cdna (< 5kb) 응용 for RT PCR Library prep Gene expression for qpcr Gene expression RNA Quantification input RNA 1 pg ~ 1 μg 1 pg ~ 1 μg 0.5 pg ~ 0.5μg 1 10 1 ~7 10 4 cells Long cdna 합성이가능한 High Quality RT kit ReverTra Ace -α- 14 kb 이상의 long cdna 합성가능 Library 제작및 RT-PCR에사용 TOFSK-101 ReverTra Ace -α- kit ReverTra Ace 5x RT buffer (contains 25 mm Mg 2+ ) RNase inhibitor (10 U/ul), dntps mixture (10 U/ul) RNase-free water, Olido (dt) 20 (10 pmol/ul) Random primers (25 pmol/ul), Control primer F (10 pmol/ul), Control primer R (10 pmol/ul), Positive control RNA (10 5 copies/ul) 100 rxn 400,000 15 분만에반응완료되는고효율 RT Kit ReverTra Ace qpcr RT Kit 적절한시약구성으로타사대비높은합성효율 RNase H 를처리하는과정이없어합성시간단축 TOFSQ-101 ReverTra Ace qpcr RT kit 5x RT buffer, Enzyme Mix (RTase, RNase inhibitor), Primer Mix (oligo dt, random primer), Nuclease-free water 200 rxns 350,000
ReverTra Ace cdna Synthesis kit 1 개의 Master Mix 타입으로간편하게 cdna 합성 ReverTra Ace qpcr RT Master Mix 간편한 protocol 과시약구성으로사용편의성증대 15 분만에 cdna 합성완료 TOFSQ-201 ReverTra Ace qpcr RT Master Mix 5x RT Master mix, 5x RT Master mix no RTcontrol, Nuclease-free water 200 rxns 360,000 Total RNA 에포함된 gdna 제거와 cdna 합성을하나의 kit 로! ReverTra Ace qpcr RT Master Mix with gdna Remover gdna 오염에의한 qpcr 결과의오류방지 타사대비월등히높은 gdna 제거율과 30 분이내의빠르고간편한실험 Genomic DNA removal (Degradation) cdna Synthesis RNA Sample Directly cdna (gdna-free) 4x DNA Master Mix with gdna Remover 37, 5 min 5x RT Master Mix Ⅱ 37,15 min / 98, 5 min Real-time PCR etc. TOFSQ-301 ReverTra Ace qpcr RT Master Mix with gdna Remover gdna remover, 4x DN Master mix, 5x RT Master mix II, 5x RT Master mix II no RT-control, Nuclease-free water 200 rxns 400,000 약 30 분만에 Total RNA 추출없이 cdna 합성완료 SuperPrep Ⅱ Cell Lysis & RT kit for qpcr Cell Lysis & DNaseⅠTreatment 5 min Directly Reverse Transcription (RT) 25 min 실험단계및시간단축으로 RNA degradation 방지 24-well 이상 high throughput cell culture 실험에최적화 Secondary, primary cell 에서도효과적 (26 종류이상 cell 에서테스트완료 ) 15 개 house keeping gene 의 purified RNA 와 qpcr 효율비교 (2.5 X 10 4 HEK293 cells) TOSCQ-401 SuperPrep Ⅱ Cell Lysis & RT kit for qpcr * Lysis Reagents (Lysis solution, gdna remover, RNase inhibitor) * RT Reagents (5x RT Mater mix, 5x RT Mater mix no RT-control Nuclease-free water) 100 rxn 1,142,100
Real-Time PCR Master Mix-One Step Kit Real Time PCR Master Mix Selection Guide One-Step qpcr Master mix Two-Step qpcr Master Mix Product Thunderbird Probe one-step qrt-pcr mix RNA-direct TM Realtime PCR Series Realtime PCR Series Thunderbird Series SYBR qpcr mix Best Performance on multiplex High efficiency High efficiency High Specificity, sensitivity Best Stability on Difficult condition Long qpcr < 1 kb < 1 kb < 2 kb GC-rich targets ++ ++ + + +++ ( 83%) Crude samples - - + + +++ Whole blood, animal & plant tissue qpcr instrument All qpcr Bio-Rad, Roche, TaKaRa, Rotor-Gene Q, StepOnePlus All qpcr Fluorescence type Probe SYBR Green / Probe SYBR Green / Probe SYBR Green 우수한민감도와 Multiplex 에특화된제품 THUNDERBIRD Probe One-Step qrt-pcr Kit 높은증폭효율로 10 copies 수준의 RNA virus 검출가능 PCR bias를낮춰다양한 target을고르게증폭하여 multiplexing qrt-pcr에효과적 dutp를포함하여 UNG 처리를통한위양성검출위험없음 TOQRZ-101 THUNDERBIRD Probe One-Step qrt-pcr kit For all qpcr 2x Reaction buffer (contains dutp) DNA polymerase, RT Enzyme mix 50x ROX reference dye, RNase free water 2 x 1.25 ml (250 rxn) 377,300 최적화된 Buffer 조성을통해증폭효율강화 RNA-direct TM Realtime PCR Series Tth polymerase로구성되어 cdna 합성과 DNA 증폭동시진행가능간소화한실험을통해샘플오염방지및재현성증가 TOQRT-101 TOQRT-201 RNA-direct TM Realtime PCR Master Mix For all qpcr RNA-direct TM SYBR Green Realtime PCR Master Mix For all qpcr 2x RNA-direct Realtime PCR Master Mix 50 nm Mn(OAc)2, Rnase free water 5 x 0.5 ml (250 rxn) 405,500 2x RNA-direct SYBR Green Realtime PCR Master Mix, 50 nm Mn(OAc)2, Rnase free water 405,500
Real Time PCR Master Mix - Two Step Kit 범용적인 qpcr Master Mix 의 Best Seller Real-time PCR Series 탁월한반응특이성으로 primer dimer 형성억제사용이편리한 master mix 타입 (ROX reference dye 포함 ) TOQPK-101 TOQPK-201 Real-time PCR Master Mix Bio-Rad, Roche, TaKaRa, Rotor-Gene Q, StepOnePlus SYBR Green Real-time PCR Master Mix Bio-Rad, Roche, TaKaRa, Rotor-Gene Q, StepOnePlus 2x Real-time PCR Master Mix 5 x 1 ml (500 rxn) 290,000 2x SYBR Green Real-time PCR Master Mix 290,000 정확성과증폭효율을모두겸비한 qpcr Master THUNDERBIRD Series 특허출원 PCR enhancer로증폭효율및 dynamic range 증가최적화된 buffer 조성으로높은검출민감도보장모든 qpcr 장비에호환가능 50X ROX reference dye 개별 vial로구성 TOQPS-101 THUNDERBIRD qpcr Probe Mix For all qpcr TOQPS-201 THUNDERBIRD qpcr SYBR Mix For all qpcr 2x Thunderbird qpcr SYBR mix, 50x ROX reference dye 3 x 1.67 ml 280,000 2x Thunderbird qpcr probe mix, 50x ROX reference dye (500 rxn) 280,000 까다로운샘플및 Target Gene 을위한 High-Quality qpcr Mix SYBR qpcr Mix SYBR qpcr Mix Amplification curve Melting curve 10 4 10 1 copy Conventional master mix (Taq based) qpcr 에서도최대 2 kb target 확인 10 4 10 1 copy 우수한내열성으로 GC-rich ( 최대 83%) target 에서도높은증폭효율보장 Directly 쥐꼬리및혈액등의샘플에서 gdna 추출없이 direct qpcr 가능 TOQKD-201 SYBR qpcr Mix For all qpcr 2x SYBR qpcr Mix 50x ROX reference dye 3 x 1.67 ml (500 rxn) 349,000 Illumina Platform 의 NGS Library qpcr Quantification Kit GenNext TM NGS Library Quantification Kit Polymerase를사용하여기존 Taq base 제품보다우수한증폭효율과정확성 High GC-/AT- Target에서도 95% 이상의높은증폭효율 6개의 Standard DNA (20 ~ 0.0002 pm, 452 bp) 를통한 R 2 값 0.99 이상의정확한 Standard curve 보장 Dilution Buffer까지 All in One으로구성하여고객편의성제공 TONLQ-101 GenNext TM NGS Library Quantification Kit For all qpcr SYBR qpcr, Mix(TOQKD-201), SYBR qpcr Mix, 50x ROX reference dye Standard & Primer Set, Standard DNA 1~6, 5x Primer Mix, 50x Dilution Buffer 500 rxn (20 ul 반응기준 ) 834,100
Immuno Enhancer Western Blot, Dot Blot 및 ELISA 실험에적용 Can Get Signal 반응조건의최적화를통해항원 - 항체반응의특이성증가 Signal-to-noise 개선하여특이 signal 향상, 비특이적 signal 및 noise 감소 Signal 향상효과로 antibody 절약사용가능 Signal 은높게, Background 는낮게도출된결과 Sample : Cultured bovine adrenal medulla cells 1. Control (H 2O) 2. Insulin (1 nm, stimulated for 5 min) 3. Insulin (10 nm, stimulated for 5 min) 4. Insulin (100 nm, stimulated for 5 min) 품번 품명 구성 규격 행사가 (VAT 별도 ) TONKB-101 Solution 1 & Solution 2 각 250 ml 289,200 Can Get Signal Immunoreaction TONKB-201 Solution 1 250 ml 162,700 Enhancer TONKB-301 Solution 2 250 ml 162,700 조직및세포에서 IHC 실험에적용 Can Get Signal Immunostain 샘플에따라테스트후적합한제품을선택하여사용가능 (IHC 용 Immunostain) Stain signal의민감도와정확도를높여더욱선명한이미지구현 <Specimen> Human brain cancer (glioblastoma) paraffin embedded section <Specimen> Drosophila culture cell line (schneider 2 (s2) cells) Conventional (1% BSA/PBSΧ Can Get Signal immunostain (Solution A) Can Get Signal immunostain (Solution B) (1) Conventional (1% BSA, 0.02% TX-100 /PBSΧ (2) Can Get Signal immunostain (Solution A) (3) Can Get Signal immunostain (Solution B) 품번 품명 구성 규격 행사가 (VAT 별도 ) TONKB-401 Starter Set Solution A & Solution B 각 5 ml 117,000 Can Get Signal TONKB-501 Solution A 20 ml 295,600 Immunostain TONKB-601 Solution B 20 ml 295,600 제품문의 02-3471-6500 / 042-824-7000 www.bmskorea.co.kr Copyrightc 2018 BMS Co., Ltd. All rights reserved. 018BM07B