2019 TOYOBO BIG SALE 기간 : 2019년 7월 1일 ~ 9월 11일까지 최대 33% SALE qpcr Enzyme PCR Enzyme cpcr Enzyme cdna Synthesis cdna Synthesis kit High Quality Enzyme Immuno Enhancer Cloning One-Step kit Can Get Signal -Plus- Mutagenesis Kit 30% Two-Step kit Can Get Signal Immunostain 10X A-attachment Mix NGS Prep & Quantification Ligation high ver.2 제품 300만원 이상 구매 시 제품 500만원 이상 구매 시 PALL Supor Acrodisc Syringe Filter Sterile 2종 + FastGene Stay Cool Rack 2개 Lab Armor Bucket Package or i-myrun II Electrophoresis System 증정 or + 0.45 um / 0.2 um, 각 1 pkg (50 ea/pkg) 증정 2 L Beads, Bead Bag, Chill Package 일시 구매 시 / 부가세 별도 문의 02-3471-6500 / 042-824-7000
DNA Polymerase Enzyme Quick Selection Guide for Effective PCR DNA Polymerase High Quality Polymerase * General PCR / Colony PCR Long PCR (Max. 23 kb) Hot Start Easy to Use (with Loading dye) Hot Start High Fidelity PCR (80-fold higher than Taq) for Cloning/ Genotyping Long PCR (Max 40 kb) / High GC/AT Target High Efficiency PCR (Tissue direct PCR) Multiplex / Bisulfite PCR Easy to use (Master Mix type) Without Loading Dye With Loading Dye One-step RT-PCR Blend Taq Blend Taq Plus Quick Taq HS DyeMix -Plus- -Plusneo FX FX Neo -Multi & Epi- One PCR Master Mix One PCR Master Mix -Blue- RT-PCR Quick Master Mix * T-vector cloning 시, A-attachment mix 사용필요 범용적으로다양한 PCR 에사용 Blend Taq Series Proofreading 기능을더해효과적인 PCR 가능한 rtaq polymerase 제품일반 Taq polymerase 보다 3 ~ 4배낮은 error rate 최대 23 kb까지증폭 Blend Taq M 1 2 3 4 5 M M 1 2 3 4 5 M M 1 2 3 4 5 M M : 1 kb Ladder Marker 1 : β-globin 1.3 kb 2 : β-globin 2.7 kb Blend Taq -Plus- 3 : β-globin 3.6 kb 4 : β-globin 17.5 kb 5 : β-globin 23.0 kb High efficient Taq DNA polymerase (Company A) Sample : Human genomic DNA Target : β-globin gene Cycling condition : 94, 2 min. 94, 30 sec. 60, 30 sec. 72, 1 min./kb 30 cycles * Hot-Start PCR 을위해 anti-taq DNA pol. antibodies 포함 Blend Taq TOBTQ-101 Blend Taq (2.5U/ul) 10x buffer 2 mm dntps 200 rxns (50 ul 반응기준 ) 102,400 원 Blend Taq -Plus- TOBTQ-201 Blend Taq -Plus- (2.5U/ul) * 10x buffer 2 mm dntps 200 rxns (50 ul 반응기준 ) 112,500 원 2X Master Mix 형태로 PCR 과 Gel Loading 까지한번에! Quick Taq HS DyeMix Hot-start PCR 로비특이적반응방지 높은증폭효율로 colony-direct PCR 가능 최대 4 kb 까지증폭 Quick Taq HS DyeMix * Hot-Start PCR 을위해 anti-taq DNA pol. antibodies 포함 TODTM-101 2x Quick Taq HS DyeMix * 100 rxns (50 ul 반응기준 ) 90,300 원 One Tube 에서 Reverse Transcription 과 PCR 을한번에! RT-PCR Quick Master Mix Company A RT-PCR Quick Master Mix 10 6 10 5 10 4 10 3 B 10 6 10 5 10 4 10 3 10 2 B 2x master mix로사용자편의성제공 RNA의 2차구조형성및 GC-rich 타겟도높은효율로증폭가능 Hot-start PCR을통한높은효율과정확성낮은 copy에서도증폭가능한높은 sensitivity Amplification of the 450-bp human G3PDH gene 450 bp RT-PCR Quick Master Mix TOPCR-311 2x RT-PCR Quick Master Mix, 50 mm Mn(OAc)2 Nuclease-free water, Positive control RNA(G3PDH mrna), Control primer F & R 50 rxns (50 ul 반응기준 ) 302,400 원 10X A-attachment Mix TOTAK-301 10 x A-attachment mix 25 rxns 78,000 원 Ligation high ver.2 TOLGK-201 Ligation high ver.2 750 ul 158,000 원
High Quality Polymerase Enzyme Thermal Stability 온도안정성 최대 7.5 배높음 Extension Rate 신장속도 약 5 배빠름 Processivity 반응의연속성 15 배이상높음 Fidelity 정확도 최대 80 배높음 Property DNA polymerase Value for indicated DNA polymerase U DNA polymerase Pfu DNA polymerase Taq DNA polymerase Origin Archaea Archaea Archaea Bacteria Thermostability (half-life) 95, 12h; 100, 3.0h 95, 12h; 100, 3.0h 95, 6h; 100, 2.9h 95, 1.6h 5' 3' exonuclease activity - - - + 3' 5' exonuclease activity + + + - Processivity (bases) > 300 > 300 < 20 ND Elongation rate (bases/sec) 106 ~138 106 ~138 25 61 Use of DNA containing uracil - + - + Taq DNA Polymerase 보다 80 배높은 PCR Fidelity One PCR Master Mix / One PCR Master Mix -Blue- -Plus- / -Plus- Neo High fidelity PCR Master Mix ( 타사 ) FX / FX Neo Long PCR enzyme ( 타사 ) Taq DNA polymerase 0 20 40 60 80 100 120 140 160 Mutation frequency (10-5 ) Number of misincorporated bases/100,000 bases) kb 당 10 초의 Ultra Fast Enzyme One TM PCR Master Mix 최대 40 kb 까지 kb 당 10 초의속도로빠른증폭가능 High Fidelity, High Efficiency enzyme Easy to use type (Master Mix) One TM PCR Master Mix One TM PCR Master Mix -Blue- M 1 2 3 4 5 6 7 M 1 2 3 4 5 6 7 M 1 2 3 4 5 6 7 타사 1 1 kb PCR condition 2 2 kb 98, 0:10 3 3 kb 60, 0:05 68, 0:05 / kb 4 4 kb 5 5 kb 6 6 kb 7 7 kb M 1 kb DNA Ladder One TM PCR Master Mix TOKMM-101 One PCR Master Mix * 200 rxns (50ul 반응기준 ) 243,800 원 One TM PCR Master Mix -Blue- TOKMM-201 One PCR Master Mix with loading dye * 200 rxns (50ul 반응기준 ) 243,800 원 x 30 * Anti- DNA pol. antibodies 포함 Multiplexing 및 Bisulfite PCR 에특화된제품 -Multi & Epi- 단일유전자증폭시, 최대 40 kb 까지증폭가능 NGS Library 합성에효과적 : Multiplex PCR 에최적화 (10 개의타겟유전자, 최대 10 kb 까지증폭가능 ) Bisulfite PCR, Bisulfite sequencing 에효과적 : Uracil(U) 포함 DNA 도인식가능한 U enzyme 사용 -Multi & Epi- TOKME-101 -Multi & Epi- (1.0U/ul) *, 2x PCR buffer for -Multi & Epi- * Anti- DNA pol. antibodies 포함 200 rxns (50 ul 반응기준 ) 305,400 원 Cloning 용 High Fidelity Enzyme -Plus- Series 일반 Taq polymerase 보다 80 배높은정확도 Buffer 조건최적화로 PCR 증폭길이와효율향상 최대 24 kb 까지증폭 * Anti- DNA pol. antibodies 포함 -Plus- TO-201 -Plus- (1.0U/ul) *, 10x PCR buffer for -Plus-, 25mM MgSO4, 2mM dntps -Plus- Neo TO-401 -Plus- Neo (1.0U/ul) *, 10x PCR buffer for -Plus- Neo, 25mM MgSO4, 2mM dntps -Plus- (1U/ul) *, 10X Buffer, 2mM -Plus- Mutagenesis Kit TOSMK-101 dntps, Dpn Ⅰ(10U/ul), Ligation high, control plasmid pak119m, control primer #1,2 200 rxns (50 ul 반응기준 ) 202,400 원 200 rxns (50 ul 반응기준 ) 202,400 원 20 rxns 248,400 원 Crude 샘플및 High GC 타겟에최적화 FX Series gdna 추출없이 direct PCR 가능 최대 40 kb 까지증폭가능 * Anti- DNA pol. antibodies 포함 FX FX Neo TOKFX-101 TOKFX-201 FX (1.0U/ul) *, 10x PCR buffer for FX, 2mM dntps FX Neo (1.0U/ul) *, 10x PCR buffer for FX Neo, 2mM dntps 200 rxns (50 ul 반응기준 ) 270,000 원 200 rxns (50 ul 반응기준 ) 270,000 원
ReverTra Ace cdna Synthesis Kit d ReverTra Ace M-MLV RTase의 RNase H domain 부위에유전자조작을시켜, RNase H activity를낮추어 mrna의 degradation을방지하여 cdna 합성효율을높임 cdna Synthesis kit Selection Guide 특성 ReverTra Ace -α- Oligo (dt) 20 & Random primer vial each ReverTra Ace qpcr RT kit Oligo (dt) 20 & Random primer Mix type ReverTra Ace qpcr RT Master Mix Master mix type ReverTra Ace qpcr RT Master Mix with gdna remover Master mix with gdna remover SuperPrep Ⅱ Cell Lysis & RT kit for qpcr Cell lysis & RT Master mix (No need RNA purification) 합성시간 25 min 15 min 30 min 30 min 합성 cdna Long cdna ( 14 kb) short-chain cdna (< 5 kb) 응용 for RT PCR Library prep Gene expression for qpcr Gene expression RNA Quantification input RNA 1 pg ~ 1 μg 1 pg ~ 1 μg 0.5 pg ~ 0.5μg 1 10 1 ~7 10 4 cells Long cdna 합성이가능한 High Quality RT kit ReverTra Ace -α- 14 kb 이상의 long cdna 합성가능 Library 제작및 RT-PCR에사용 ReverTra Ace -a- TOFSK-101 - ReverTra Ace - 5x RT buffer (contains 25mM Mg2+) - RNase inhibitor (10 U/ul) - dntps mixture (10 U/ul) - RNase-free water - Olido (dt)20 (10 pmol/ul) - Random primers (25 pmol/ul) - Control primer F (10 pmol/ul) - Control primer R (10 pmol/ul) - Positive control RNA (10 5 copies/ul) 100 rxns 400,000 원 15 분만에반응완료되는고효율 RT Kit ReverTra Ace qpcr RT Kit 적절한시약구성으로타사대비높은합성효율 RNase H 를처리하는과정이없어합성시간단축 ReverTra Ace qpcr RT kit TOFSQ-101-5x RT buffer - Enzyme Mix (RTase, RNase inhibitor) - Primer Mix (oligo dt, random primer) - Nuclease-free water 200 rxns 350,000 원
ReverTra Ace cdna Synthesis Kit 1 개의 Master Mix 타입으로간편하게 cdna 합성 ReverTra Ace qpcr RT Master Mix 간편한 protocol 과시약구성으로사용편의성증대 15 분만에 cdna 합성완료 ReverTra Ace qpcr RT Master Mix TOFSQ-201-5x RT Master mix - 5x RT Master mix no RT-control - Nuclease-free water 200 rxns 360,000 원 Total RNA 에포함된 gdna 제거와 cdna 합성을하나의 kit 로! ReverTra Ace qpcr RT Master Mix with gdna Remover Genomic DNA removal (Degradation) RNA Sample Directly 4x DNA Master Mix with gdna Remover 37, 5 min gdna 오염에의한 qpcr 결과의오류방지 타사대비월등히높은 gdna 제거율과 30 분이내의빠르고간편한실험 cdna Synthesis cdna (gdna-free) 5x RT Master Mix Ⅱ 37,15 min / 98, 5 min Real-time PCR etc. ReverTra Ace qpcr RT Master Mix with gdna Remover TOFSQ-301 - gdna remover - 4x DN Master mix - 5x RT Master mix II - 5x RT Master mix II no RT-control - Nuclease-free water 200 rxns 400,000 원 Total RNA 추출없이 cdna 합성을약 30 분만에완료 SuperPrep Ⅱ Cell Lysis & RT kit for qpcr Cell Lysis & DNaseⅠTreatment 5 min Directly Reverse Transcription (RT) 25 min 실험단계및시간단축으로 RNA degradation 방지 24-well 이상 high throughput cell culture 실험에최적화 Primary, Secondary cell 에서도효과적 (26 종류이상 cell 에서테스트완료 ) 15 개 house keeping gene 의 purified RNA 와 qpcr 효율비교 (2.5 X 10 4 HEK293 cells) SuperPrep ⅡCell Lysis & RT kit for qpcr TOSCQ-401 * Lysis Reagents (Lysis solution, gdna remover, RNase inhibitor) * RT Reagents 5x RT Mater mix 5x RT Mater mix no RT-control Nuclease-free water 100 rxns 1,142,100 원
Immuno Enhancer Real-Time PCR Master Mix One Step Kit Western Blot, Dot Blot 및 ELISA 실험에적용 Can Get Signal 반응조건의최적화를통해항원-항체반응의특이성증가 Signal-to-noise 개선하여특이 signal 향상, 비특이적 signal 및 noise 감소 Signal 향상효과로 antibody 절약사용가능 품명 품번 구성 규격 행사가 (VAT 별도 ) Can Get Signal Immunoreaction Enhancer Solution TONKB-101 Solution 1 & Solution 2 각 250 ml 289,200원 Can Get Signal Immunoreaction Enhancer Solution 1 TONKB-201 Solution 1 250 ml 162,700원 Can Get Signal Immunoreaction Enhancer Solution 2 TONKB-301 Solution 2 250 ml 162,700원 조직및세포에서 IHC 실험에적용 Can Get Signal Immunostain 샘플에따라테스트후적합한제품을선택하여사용가능 Stain signal 의민감도와정확도를높여더욱선명한이미지구현 품명 품번 구성 규격 행사가 (VAT 별도 ) Can Get Signal Immunostain Starter Set TONKB-401 Solution A & Solution B 각 5 ml 117,000원 Can Get Signal Immunostain Solution A TONKB-501 Solution A 20 ml 295,600원 Can Get Signal Immunostain Solution B TONKB-601 Solution B 20 ml 295,600원 우수한민감도와 Multiplex 에특화된제품 THUNDERBIRD Probe One-Step qrt-pcr Kit RNA virus 검출민감도 1 x 10 1 copies PCR bias를낮춰다양한 target을고르게증폭하여 multiplexing qrt-pcr에효과적 dutp를포함하여 UNG 처리를통한위양성검출위험없음 THUNDERBIRD Probe One-Step qrt-pcr kit TOQRZ-101-2X Reaction buffer (contains dutp) - DNA polymerase - RT Enzyme mix - 50X ROX reference dye - RNase free water 100 rxns (50 ul 반응기준 ) 377,300 원 최적화된 Buffer 조성을통해증폭효율강화 RNA-direct TM Realtime PCR Series Tth polymerase 로구성되어 cdna 합성과 DNA 증폭동시진행가능 간소화한실험을통해샘플오염방지및재현성증가 RNA-direct TM Realtime PCR Master Mix RNA-direct TM SYBR Green Realtime PCR Master Mix TOQRT-101 TOQRT-201-2X RNA-direct Realtime - PCR Master Mix - 50nM Mn(OAc)2 - RNase free water - 2X RNA-direct SYBR - Green Realtime PCR - Master Mix - 50nM Mn(OAc)2 - RNase free water 100 rxns (50 ul 반응기준 ) 100 rxns (50 ul 반응기준 ) 405,500 원 405,500 원
Real-Time PCR Master Mix One Step Kit Real Time PCR Master Mix Selection Guide One-Step qpcr Master mix Two-Step qpcr Master Mix Product Thunderbird Probe one-step qrt-pcr mix RNA-direct TM Realtime PCR Series Realtime PCR Series Thunderbird Series SYBR qpcr mix Best Performance on multiplex High efficiency High efficiency High Specificity, sensitivity Best Stability on Difficult condition Reference dye Separate vial (50X ROX) ROX Included ROX Included Separate vial (50X ROX) qpcr instrument All qpcr Bio-Rad, Roche, TaKaRa, Rotor-Gene Q, StepOnePlus Bio-Rad, Roche, TaKaRa, Rotor-Gene Q, StepOnePlus All qpcr Fluorescence type Probe Only SYBR Green / Probe SYBR Green / Probe SYBR Green / Probe SYBR Green Only 범용적인 qpcr Master Mix 의 Best Seller Real-time PCR Series 탁월한반응특이성으로 primer dimer 형성억제사용이편리한 master mix 타입 (ROX reference dye 포함 ) Real-time PCR Master Mix TOQPK-101 2X Real-time PCR Master Mix 5 x 1 ml (500 rxn) 290,000 원 SYBR Green Real-time PCR Master Mix TOQPK-201 2X SYBR Green Real-time PCR Master Mix 5 x 1 ml (500 rxn) 290,000 원 정확성과증폭효율을모두겸비한 qpcr Master Mix THUNDERBIRD Series 특허출원 PCR enhancer로증폭효율및 dynamic range 증가최적화된 buffer 조성으로높은검출민감도보장모든 qpcr 장비에호환가능 50X ROX reference dye 개별 vial로구성 THUNDERBIRD qpcr Probe Mix THUNDERBIRD qpcr SYBR Mix TOQPS-101 TOQPS-201 2X THUNDERBIRD qpcr Probe Mix 50X ROX reference dye 3 x 1.67 ml (500 rxns) 280,000 원 2X THUNDERBIRD qpcr SYBR Mix 50X ROX reference dye 3 x 1.67 ml (500 rxns) 280,000 원 까다로운샘플및 Target Gene 을위한 High-Quality qpcr Mix SYBR qpcr Mix SYBR qpcr Mix Amplification curve 10 4 10 1 copy Conventional master mix (Taq based) 10 4 10 1 copy qpcr 에서도최대 2 kb target 확인 우수한내열성으로 GC-rich ( 최대 83%) target 에서도높은증폭효율보장 쥐꼬리및혈액등의샘플에서 gdna 추출없이 direct qpcr 가능 Directly Melting curve SYBR qpcr Mix TOQKD-201 2X SYBR qpcr Mix 50X ROX reference dye 3 x 1.67 ml (500 rxns) 349,000 원
Illumina Platform NGS Library Prep & Quantification Kit 2 시간이내에빠르고간편하게 library 제작가능 GenNext TM NGS Library Prep Kit U DNA polymerase, PCR enhancer 사용으로 Low bias, Ultra Fast PCR 가능 GC-contents 83% 이상의샘플도고효율로증폭가능 1 ng 극소량의샘플에서도높은 yield 의 library 제작가능 (Shared DNA Input 1 ng ~ 1 μg ) High GC target, FFPE 포함모든샘플에서도고품질 Library 제작가능 (Adapters, beads 별도구매 ) Human genomic DNA 1 ng / 11 cycles Human genomic DNA 10 ng / 8 cycles Human genomic DNA 100 ng / 5 cycles E coli genomic DNA 1 ug / PCR Free UM 1500 1000 800 700 600 500 UM 1500 1000 800 700 600 500 UM 1500 1000 800 700 600 500 UM 1500 1000 800 700 600 500 400 400 400 400 300 300 300 300 200 200 200 200 100 100 100 100 Yield (nm) M 1 2 Yield (nm) Yield (nm) Yield (nm) M 1 2 M 1 2 M 1 2 M : 100 bp DNA Ladder / 1 : GenNext TM NGS Library Prep Kit / 2 : 타사 GenNext TM NGS Library Prep Kit 타사 30% GenNext TM NGS Library Prep Kit TOLPK-101T - End repair and A tailing buffer 8 rxns 296,400원 TOLPK-101 - End repair and A tailing enzyme - Ligation solution 24 rxns 585,600원 TOLPK-101L - Library amplification master mix - Library amplification primer mix 96 rxns 1,850,500원 High GC-/AT- Target 에서도 95% 이상의높은증폭효율보장 GenNext TM NGS Library Quantification Kit Polymerase를사용하여기존 Taq base 제품보다우수한증폭효율과정확성보장 6개의 Standard DNA (20 ~ 0.0002 pm, 452 bp) 를통해 R 2 값 0.99 이상의정확한 Standard curve 제공 Dilution Buffer까지 All in One으로구성하여고객편의성제공 GenNext TM NGS Library Quantification Kit Melt Curve 105% Influence of GC content 타사 A Melt Curve 100% PCR efficiency 95% 90% 85% 80% 타사 B Melt Curve 75% S : Standard DNA (20 pm ~ 0.0002 pm) L : Diluted Library Sample 70% * * * Standard STD2 27.3% 69.5% 80.3% 83% 3.0% DNA* (296bp) (450bp) (294bp) (188bp) (Size) 30% GenNext TM NGS Library Quantification Kit TONLQ-101 - SYBR qpcr Mix (TOQKD-201) ( SYBR qpcr Mix / 50X ROX reference dye) - Standard & Primer Set (Standard DNA 1 ~ 6 / 5X Primer Mix / 50X Dilution Buffer) 500 rxns (20 ul 반응기준 ) 583,800 원 문의 02-3471-6500 / 042-824-7000 Copyrightc 2019 BMS Co., Ltd. All rights reserved. 019BM06A