프로메가 2018 여름이벤트 BIG SALE 행사기간 2018년 8월 06일 ~ 9월 14일 *본 행사지에 표기된 금액은 부가세 별도 금액입니다. *단가 계약 및 입찰은 행사에서 제외됩니다.
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1 프로메가 2018 여름이벤트 BIG SALE 행사기간 2018년 8월 06일 ~ 9월 14일 *본 행사지에 표기된 금액은 부가세 별도 금액입니다. *단가 계약 및 입찰은 행사에서 제외됩니다.
2 EtBr 을아직사용하신다면? 일회용 Gel extractor 를사용해보세요! 내몸은소중하니깐 ~~~ 15ul minimum elution 이가능합니다!! 프로메가 Steady seller "T-vector"!! A260/A230 ratio 가 으로안정적이고, ethanol 을사용하지않아, NGS 목적에적합합니다. 프로메가 FFPE kit 는 xylene 을사용하지않고, mineral oil 을사용하여파라핀을제거합니다. Mini Midi Maxi EtBr 구매하세요? 위의 X-tracta 를같이추천드립니다!! Clean-up A2121 x-tracta Gel Extractor, 25/pack 47,000 24,000 A2122 x-tracta Gel Extractor, 100/pack 146,000 73,000 A9281 Wizard SV Gel and PCR Clean-up System, 50 preps 116,000 58,000 A9282 Wizard SV Gel and PCR Clean-up System, 250 preps 476, ,000 A9285 Wizard SV Gel and PCR Clean-up System, 1,000 preps 1,521, ,000 Cloning A1360 pgem -T Easy Vector System I 252, ,000 A1380 pgem -T Easy Vector System II 422, ,000 A3600 pgem -T Vector System I 207, ,000 A3610 pgem -T Vector System II 380, ,000 M1801 T4 DNA Ligase, 100u 73,000 55,000 25% M1804 T4 DNA Ligase, 500u 291, ,000 M8221 LigaFast Rapid DNA Ligation System, 30 rxn 126,000 95,000 M8225 LigaFast Rapid DNA LigationSystem, 150 rxn 412, ,000 V3941 X-Gal, 100mg 133,000 67,000 V3951 IPTG, Dioxane-Free, 5g Fibrous tissue에사용가능한 lysis 324, ,000 V3955 IPTG, Dioxane-Free, 1g buffer가별도로들어있습니다. 87,000 44,000 gdna A2051 ReliaPrep gdna Tissue Miniprep System, 100 Preps 388, ,000 A2052 ReliaPrep gdna Tissue Miniprep System, 250 Preps 886, ,000 A5081 ReliaPrep Blood gdna Miniprep System, 100 Preps 317, ,000 25% A5082 ReliaPrep Blood gdna Miniprep System, 250 Preps 725, ,000 A2352 ReliaPrep FFPE gdna Miniprep System, 100 Preps 465, ,000 A1120 Wizard Genomic DNA Purification Kit, 100 Isolations 240, ,000 A1125 Wizard Genomic DNA Purification Kit, 500 Isolations 600, ,000 Solution type A1620 Wizard Genomic DNA Purification Kit, 100 isolations 10ml 1,481,000 1,185,000 A2360 Wizard SV Genomic DNA Purification System, 50 preps 176, ,000 A2361 Wizard SV Genomic DNA Purification System, 250 preps 728, ,000 Plasmid purification A1330 Wizard Plus SV Minipreps DNA Purification System, 50 preps 103,000 52,000 A1460 Wizard Plus SV Minipreps DNA Purification System, 250 preps 417, ,000 A1222 PureYield Plasmid Miniprep System, 250 preps 631, ,000 A1223 PureYield Plasmid Miniprep System, 100 preps Vacuum방법에필요한 manifold, 282, ,000 A2492 PureYield Plasmid Midiprep System, 25 preps eluator 그리고 pump까지모두 294, ,000 A2495 PureYield Plasmid Midiprep System, 100 Preps 포함되어있습니다. 1,079, ,000 A2392 PureYield Plasmid Maxiprep System, 10 preps 253, ,000 A2393 PureYield Plasmid Maxiprep System, 25 preps 598, ,000 A6742 PureYield Plasmid Midiprep Start-Up Kit, 50 prep 1,506,000 1,205,000 A6732 PureYield Plasmid Maxiprep Start-Up Kit, 100 preps 1,621,000 1,297,000 Marker G3191 RNA Markers, 50ul 197,000 99,000 G bp DNA Step Ladder, 32.5ug (50 lanes) 192,000 96,000 G bp DNA Step Ladder, 100ug (55 lanes) 192,000 96,000 G bp DNA Step Ladder, 340ug/ml 90ug (52 lanes) 225, ,000 G5711 DNA Ladder, 1kb, 500ul (100 lanes) 203, ,000 G bp DNA Step Ladder, 100ug (100 lanes) 280, ,000 G7521 BenchTop pgem DNA Markers, 250ul 238, ,000 G7541 BenchTop 1kb DNA Ladder, 600ul 224, ,000 G8291 BenchTop 100bp DNA Ladder, 300ul (50 lanes) 198,000 99,000 H5041 Ethidium Bromide Solution, 10ml 70,000 35,000 dntp C1141 PCR Nucleotide Mix, 10mM, 200ul 111,000 78,000 C1145 PCR Nucleotide Mix, 10mM, 1,000ul 428, ,000 U dntp's, 40uMoles each 473, ,000 U1511 dntp Mix, 10mM, 200ul 38,000 27,000 U1515 dntp Mix, 10mM, 1,000ul 90,000 63,000 어머!! 이런건이때사다놓으세요 ~~ 최고성능의 Miniprep kit! 할인가로경험해보세요. 할인할때프로메가마커한번사용해보세요 ~~
3 Dye-based Probe-based RT mix 에는 RNasin Plus RNase inhibitor 가포함되어있어, 따로 inhibitor 처리를하지않아도되요 ~~ 기존에 M-MLV, AMV, ImProm-II 를사용했었다면, 성능과편리성이향상된 GoScript RT mix 를사용해보시는건어떨까요? 이제품은 RT 를 15 분만에할수있는 Great faster 제품입니다!! 요제품은 ~~ One-step RT-PCR 제품입니다 ~ Inhibitor 를사용하시다니!! 철저히 RNA 보호를하시는군요? 그런데 RT mix 에는 inhibitor 가포함되어있어별도로사용할필요가없다는사실!! 을아시나요? 유기용매를사용하지않고, mirna 를다양한샘플에서분리정제할수있는제품입니다. 또한 mirna 이외의 small RNA 분리정제용으로도사용할수있어요. Real-time PCR A6001 GoTaq qpcr Master Mix, 5ml (5 x 1ml) 294, ,000 A6002 GoTaq qpcr Master Mix, 25ml (25 x 1ml) 1,375, ,000 A6010 GoTaq 2-Step RT-qPCR System 200 rxn 624, ,000 A6020 GoTaq 1-Step RT-qPCR System, 200 rxn 684, ,000 A6100 GoTaq Probe qpcr Master Mix, 100 rxn 65,000 46,000 A6101 GoTaq Probe qpcr Master Mix, 200 rxn 168, ,000 A6102 GoTaq Probe qpcr Master Mix, 1,000 rxn 731, ,000 A6110 GoTaq Probe 2-Step RT-qPCR System, 200 rxn 400, ,000 A6120 GoTaq Probe 1-Step RT-qPCR System, 200 rxn 253, ,000 Reverse Transcription enzyme A2790 GoScript Reverse Transcription Mix, Oligo(dT), 50 rxn 326, ,000 A2791 GoScript Reverse Transcription Mix, Oligo(dT), 100 rxn 436, ,000 A2800 GoScript Reverse Transcription Mix, Random Primers, 50 rxn 326, ,000 25% A2801 GoScript Reverse Transcription Mix, Random Primers, 100 rxn 436, ,000 A5000 GoScript Reverse Transcription System, 50 rxn 326, ,000 A5001 GoScript Reverse Transcription System, 100 rxn 628, ,000 A5003 GoScript Reverse Transcriptase, 100 rxn 187, ,000 A5004 GoScript Reverse Transcriptase, 500 rxn 720, ,000 A3800 ImProm-II Reverse Transcription System, 100 rxn 718, ,000 A3802 ImProm-II Reverse Transcriptase, 100 rxn 198, ,000 A3803 ImProm-II Reverse Transcriptase, 500 rxn 780, ,000 M1701 M-MLV Reverse Transcriptase, 10,000u 99,000 80,000 M1705 M-MLV Reverse Transcriptase, 50,000u 374, ,000 M5101 AMV Reverse Transcriptase, 300u 148, ,000 M5108 AMV Reverse Transcriptase, 1,000u 398, ,000 A3500 Reverse Transcription System 719, ,000 One-step RT-PCR A1701 AccessQuick RT-PCR System, 20 rxn 184, ,000 A1702 AccessQuick RT-PCR System, 100 rxn 707, ,000 RNase inhibitor N2111 RNasin RNase Inhibitor, 2,500u 187, ,000 N2115 RNasin RNase Inhibitor 10,000u 491, ,000 N2511 Recombinant RNasin RNase Inhibitor, 2,500u 162, ,000 N2515 Recombinant RNasin RNase Inhibitor, 10,000u 415, ,000 N2611 RNasin Plus RNase Inhibitor, 2500u 164, ,000 N2615 RNasin Plus RNase Inhibitor, 10,000u 494, ,000 RT Primer C1101 Oligo(dT) 15 Primer, 500ug/ml 20ug 83,000 67,000 C1181 Random Primers, 20ug 53,000 43,000 RNA purification_dnase M6101 RQ1 RNase-Free DNase, 1,000u 76,000 61,000 RNA purification Z6011 ReliaPrep RNA Cell Miniprep System, 50 preps 308, ,000 Z6012 ReliaPrep RNA Cell Miniprep System, 250 preps 1,375,000 1,032,000 25% Z6111 ReliaPrep RNA Tissue Miniprep System, 50 preps 308, ,000 Z6112 ReliaPrep RNA Tissue Miniprep System, 250 preps 1,375,000 1,032,000 Z1002 ReliaPrep FFPE Total RNA Miniprep System, 100 preps 881, ,000 Z6211 ReliaPrep mirna Cell and Tissue Miniprep System, 50 preps 460, ,000 Z3100 SV Total RNA Isolation System, 50 preps 294, ,000 Z3105 SV Total RNA Isolation System, 250 preps 1,307,000 1,046,000 ReliaPrep RNA Kit 의 column 은요렇게생겼어요! Membrane 의직경이좁아 10-15ul 농축을할수있답니다 ~~
4 모든 G2 Polymerase 는 할인입니다 ~~ 아시나요? 모든 GoTaq 제품에는 gel loading dye 가포함된 Green buffer 와 loading dye 가없는 Colorless buffer 두종류가들어있습니다. 실험목적에맞게요렇게저렇게사용하세요 ~~ 아 ~~~ 그래서 master mix 가 Green 과 Colorless 두종류였군요? ㅎㅎ Purity 확인은흡광방법으로! 정량은형광방법인 Quantus 로진행하세요 ~ 저는 DNA 에만붙는답니다 ~~ 저는 RNA 에만붙는답니다 ~~ Taq DNA polymerase M7841 GoTaq G2 DNA Polymerase, 100u 49,000 35,000 M7845 GoTaq G2 DNA Polymerase, 500u Routine PCR용폴 ~~ 폴 ~~ 151, ,000 M7848 GoTaq G2 DNA Polymerase, 2,500u 661, ,000 M7801 GoTaq G2 Flexi DNA Polymerase, 100u 49,000 35,000 M7805 GoTaq G2 Flexi DNA Polymerase, 500u 151, ,000 내맘대로 MgCl 2 를조절해야지폴 ~~ M7806 GoTaq G2 Flexi DNA Polymerase, 2,500u 661, ,000 M7808 GoTaq G2 Flexi DNA Polymerase, 10,000u 2,240,000 1,568,000 M7822 GoTaq G2 Green Master Mix, 100 rxn 54,000 38,000 M7823 GoTaq G2 Green Master Mix, 1,000 rxn 448, ,000 편리한 master mix 타입폴 ~~ M7832 GoTaq G2 Colorless Master Mix, 100 rxn 54,000 38,000 M7833 GoTaq G2 Colorless Master Mix, 1,000 rxn 448, ,000 M7401 GoTaq G2 Hot Start Polymerase, 100u 81,000 57,000 M7405 GoTaq G2 Hot Start Polymerase, 500u 잡밴드가많다구요? 252, ,000 M7406 GoTaq G2 Hot Start Polymerase, 2,500u 그렇다면 Hot start 폴 ~~ 1,127, ,000 M7408 GoTaq G2 Hot Start Polymerase, 10,000u 4,200,000 2,940,000 M7422 GoTaq G2 Hot Start Green Master Mix, 100 rxn 90,000 63,000 M7423 GoTaq G2 Hot Start Green Master Mix, 1,000 rxn Hot start 709, ,000 M7432 GoTaq G2 Hot Start Colorless Master Mix, 100 rxn master mix 폴 ~~ 90,000 63,000 M7433 GoTaq G2 Hot Start Colorless Master Mix, 1,000 rxn 709, ,000 M3001 GoTaq DNA Polymerase, 100u 67,000 54,000 M3005 GoTaq DNA Polymerase, 500u 258, ,000 M3008 GoTaq DNA Polymerase, 2500u 1,220, ,000 M8291 GoTaq Flexi DNA Polymerase, 100u 51,000 41,000 M8295 GoTaq Flexi DNA Polymerase, 500u 212, ,000 M8296 GoTaq Flexi DNA Polymerase, 2500u 940, ,000 M7122 GoTaq Green Master Mix, 100 rxn 65,000 52,000 M7123 GoTaq Green Master Mix, 1,000 rxn 543, ,000 M7132 GoTaq Colorless Master Mix, 100 rxn 65,000 52,000 M7133 GoTaq Colorless Master Mix, 1,000 rxn 543, ,000 M5001 GoTaq Hot Start Polymerase, 100u 107,000 86,000 M5005 GoTaq Hot Start Polymerase, 500u 464, ,000 M5008 GoTaq Hot Start Polymerase, 10,000u 6,790,000 5,432,000 M5122 GoTaq Hot Start Green Master Mix, 100 rxn 145, ,000 M5123 GoTaq Hot Start Green Master Mix, 1,000 rxn 1,146, ,000 M5132 GoTaq Hot Start Colorless Master Mix, 100 rxn 145, ,000 M5133 GoTaq Hot Start Colorless Master Mix, 1,000 rxn 1,146, ,000 Special Taq Target 유전자가 5 kb가넘는다고요? 그렇다면롱 ~ 폴 ~ M4021 GoTaq Long PCR Master Mix 241, ,000 M7741 Pfu DNA Polymerase, 100u 159, ,000 M7745 Pfu DNA Polymerase, 500u 586, ,000 Quantitation Mismatching을수정해주는 proofreading기능이있답니다 ~~ E2670 QuantiFluor dsdna System 377, ,000 E3190 QuantiFluor ssdna System 425, ,000 E3310 QuantiFluor RNA System 425, ,000 E4870 QuantiFluor ONE dsdna System, 500rxn ONE 은희석할필요가없는 438, ,000 E4871 QuantiFluor ONE dsdna System, 100rxn 간편한제품입니다 149, ,000 25% E mL PCR Tubes, 200 pack NGS Starter는 Quantus와 QuantiFluor ONE dsdna, 104,000 78,000 E6150 Quantus Fluorometer 500rxn 시약이포함된패키지제품이에요 2,839,000 2,130,000 E5150 Quantus NGS Starter Package 2,860,000 2,145,000 Restriction enzyme 전품목 할인 제한효소리스트는프로메가홈페이지에서확인하세요 ~~
5 몸에해로운 EtBr 대신사용할수있는 fluorescent dye 에요 ~~ 기존사용하셨던 UV Transilluminator 로확인할수있답니다! SDS-polyacrylamide gel 만들때필요한 chemical 입니다 ~~ 미리미리구비해두세요!! Western blot. washing 할때필요하시죠? 앗!! Induction 해야할시간이에요!! Lag Log Stationary 프로메가에도 G-418 이있답니다 ~~ 모르셨죠? Biochemical V3121 Agarose, LE, Analytical Grade, 100g 255, ,000 V3125 Agarose, LE, Analytical Grade, 500g 689, ,000 V2111 Agarose, LMP, Analytical Grade, 25g 237, ,000 V2831 Agarose, LMP, Preparative Grade for Large Fragments, 25g 323, ,000 V3841 Agarose, LMP, Preparative Grade for Small Fragments, 25g 209, ,000 H1181 Diamond Nucleic Acid Dye, 500ul 105,000 53,000 H5001 Boric Acid, 500g 94,000 47,000 H5003 Boric Acid 1000g 148,000 74,000 H5031 EDTA Disodium Salt 100g 73,000 37,000 H5032 EDTA Disodium Salt 500g 192,000 96,000 H5051 Formamide, 100ml 102,000 51,000 H5052 Formamide, 500ml 218, ,000 H5071 Glycine 500g 126,000 63,000 H5073 Glycine, 1kg 167,000 84,000 H5113 Sodium Dodecyl Sulfate(SDS), 100g 111,000 56,000 H5114 Sodium Dodecyl Sulfate(SDS), 500g 253, ,000 H5115 Sodium Dodecyl Sulfate(SDS), 1kg 549, ,000 V6551 Sodium Dodecyl Sulfate(SDS) Solution, 100ml 64,000 32,000 V6553 Sodium Dodecyl Sulfate(SDS) Solution, 500ml 294, ,000 H5121 Tris-HCl (Tris-Hydrochloride), 100g 85,000 43,000 H5123 Tris-HCl (Tris-Hydrochloride), 500g 196,000 98,000 H5125 Tris-HCl (Tris-Hydrochloride), 2500g 867, ,000 V3111 Acrylamide Molecular Grade, 100g 83,000 42,000 V3115 Acrylamide Molecular Grade, 500g 250, ,000 V3131 Ammonium Persulfate, 25g (APS) 34,000 17,000 V3141 Bisacrylamide, 25g (N,N'-Methylenebisacrylamide) 52,000 26,000 V3143 Bisacrylamide, 125g (N,N'-Methylenebisacrylamide) 162,000 81,000 H5151 Tween 20, 500ml 143,000 72,000 H5152 Tween 20, 100ml 97,000 49,000 H5131 Tris Base, 500g 146,000 73,000 H5133 Tris Base, 100g 46,000 23,000 H5135 Tris Base, 2,500g 558, ,000 H5141 Triton X ml 161,000 81,000 H5142 Triton X ml 79,000 40,000 H5252 Ammonium Sulfate 5kg 228, ,000 H5271 Sodium Chloride 500g 60,000 30,000 H5273 Sodium Chloride 1kg 105,000 53,000 H5302 HEPES (free acid), 100g 116,000 58,000 H5303 HEPES (free acid), 500g 471, ,000 H5433 Glycerol, 1000ml 89,000 45,000 S2011 Coelenterazine h, 250ug 193,000 97,000 V3011 PEG 8000 Powder (Polyethylene Glycol), 500g 122,000 61,000 V3151 DTT, 5g (Dithiothreitol) 142,000 71,000 V3155 DTT, 25g (Dithiothreitol) 508, ,000 V3171 Urea, 1kg 105,000 53,000 V3175 Urea, 5kg 344, ,000 V3951 IPTG, Dioxane-Free, 5g 324, ,000 V3955 IPTG, Dioxane-Free, 1g 87,000 44,000 V4221 5M Sodium Chloride, 1,000ml 71,000 36,000 V M EDTA, ph 8.0, 100ml 22,000 11,000 V M EDTA, ph 8.0, 400ml 88,000 44,000 V4251 TBE Buffer, 10X Molecular Biology Grade, 1,000ml 97,000 49,000 V4261 SSC Buffer, 20X, 1L 108,000 54,000 V4271 TAE Buffer, 10X, 1,000ml 91,000 46,000 V4281 TAE, 40X (Tris-acetate-EDTA), 1,000ml 149,000 75,000 V6231 TE Buffer, 1X, 100ml 49,000 25,000 V6232 TE Buffer, 1X, 500ml 194,000 97,000 V7983 Antibiotic G-418 Sulfate, 5g 739, ,000 V8091 Antibiotic G-418 Sulfate Solution, 20ml 232, ,000
6 MTS assay MTT assay 세포환원력을이용하여최대 72 시간까지 real-time 으로세포의 viability 를발광기법으로측정하는제품입니다. LDH assay DNA 염색하는형광 dye 기법으로최대 72 시간까지 real-time 으로세포독성변화모니터링이가능합니다. PS-Annexin V 결합을 microplate 에서최대 48 시간까지 real-time 으로모니터링할수있습니다. Cell Viability Assay G3580 CellTiter 96 AQueous One Solution Cell Proliferation Assay, 1,000 assays 382, ,000 G3581 CellTiter 96 AQueous One Solution Cell Proliferation Assay, 5,000 assays 1,260, ,000 G5421 CellTiter 96 AQueous Non-Radioactive Cell Proliferation Assay, 1,000 assays 345, ,000 G5430 CellTiter 96 AQueous Non-Radioactive Cell Proliferation Assay, 5,000 assays 1,140, ,000 35% G4000 CellTiter 96 Non-Radioactive Cell Proliferation Assay, 1,000 assays 374, ,000 G4100 CellTiter 96 Non-Radioactive Cell Proliferation Assay, 5,000 assays 1,110, ,000 G7570 CellTiter-Glo Luminescent Cell Viability, 10ml 160, ,000 G7571 CellTiter-Glo Luminescent Cell Viability, 10 x 10ml 728, ,000 G7572 CellTiter-Glo Luminescent Cell Viability, 100ml 576, ,000 G7573 CellTiter-Glo Luminescent Cell Viability, 10 x 100ml 4,712,000 3,770,000 G9241 CellTiter-Glo 2.0 Assay, 10ml 169, ,000 G9242 CellTiter-Glo 2.0 Assay, 100ml 620, ,000 G9243 CellTiter-Glo 2.0 Assay, 500ml 2,630,000 2,104,000 G9681 CellTiter-Glo 3D Cell Viability Assay, 10ml 173, ,000 G9682 CellTiter-Glo 3D Cell Viability Assay, 10 x 10ml 803, ,000 G9683 CellTiter-Glo 3D Cell Viability Assay, 100ml 624, ,000 G9711 RealTime-Glo MT Cell Viability Assay, 100 reactions 213, ,000 G9712 RealTime-Glo MT Cell Viability Assay, 10 x 100 reactions 1,040, ,000 25% G9713 RealTime-Glo MT Cell Viability Assay, 1,000 reactions 941, ,000 Cytotoxicity Assay G1780 CytoTox 96 Non-Radioactive Cytotoxicity Assay, 1,000 assays 405, ,000 G8741 CellTox Green Cytotoxicity Assay, 10ml 159, ,000 G8742 CellTox Green Cytotoxicity Assay, 50ml Apoptosis Inflammation Assay 580, ,000 G8743 CellTox Green Cytotoxicity Assay, 100ml 923, ,000 25% G8731 CellTox Green Express Cytotoxicity Assay, 200ul 867, ,000 Apoptosis Assay G8090 RealTime-Glo Caspase-Glo Annexin 3/7 Assay, 2.5ml V Apoptosis and 181, ,000 G8091 Necrosis Caspase-Glo Assay 3/7 Assay, 10ml 566, ,000 G8092 A No-Wash, Caspase-Glo One-Step 3/7 Method Assay, for 100ml Real-Time Apoptosis Detection 2,960,000 2,368,000 G8200 Caspase-Glo 8 Assay, 2.5ml 181, ,000 G8201 Caspase-Glo 8 Assay, 10ml 566, ,000 This simple, non-lytic assay allows real-time monitoring of apoptosis progression, without the need for multiple plates, G8210 complicated processing, Caspase-Glo or specialized detection 9 Assay, equipment. 2.5ml A multimode plate reader capable of detecting luminescence 181, ,000 and fluorescence is the only instrument required. G8211 Caspase-Glo 9 Assay, 10ml 566, ,000 The RealTime-Glo JA1011 RealTime-Glo Apoptosis and Necrosis Assay measures exposure of phosphatidylserine (PS) on the outer leaflet of the cell membrane during the apoptotic process, Annexin and loss of membrane V Apoptosis integrity during and secondary Necrosis necrosis. Annexin Assay, V 100 assays 681, ,000 binding is detected with a simple luminescence signal, and necrosis detected with a fluorescent DNA binding dye. JA1012 RealTime-Glo Annexin V Apoptosis and Necrosis Assay, 1,000 assays 3,405,000 2,554,000 25% Continually Monitors Cell State JA1000 Repeated measures RealTime-Glo of luminescence and fluorescence Annexin from the same V Apoptosis well during compound Assay, exposure 100 allow determination assays 585, ,000 of compound potency using far fewer plates and less reagents than endpoint assays. JA1001 RealTime-Glo Annexin V Apoptosis Assay, 1,000 assays 2,925,000 2,194,000 Well tolerated by a variety of cultured cell types. G3250 Allows real-time DeadEnd measurement of the Fluorometric dose and time-dependent TUNEL magnitude System, of apoptotic progression. 60 reactions 780, ,000 Can be multiplexed with other assays to gain more information on mechanism of toxicity. G7130 DeadEnd Colorimetric TUNEL System, 40 reactions 768, ,000 Plate-Based and Scalable G7360 DeadEnd Colorimetric TUNEL System, 20 reactions 557, ,000 Since the reagent is added directly to cells in culture, the assay can be conducted in microplate formats without onerous washing or processing steps. Glo 제품은 lysis 기능이시약내에이미포함되어있는별도의 lysis 단계가필요없는 homogenous 방법으로발광 signal 이긴제품입니다. Easy to Use Simply add assay reagent to cells at plating or dosing and read repeatedly. RealTime-Glo Annexin V Apoptosis & Necrosis Assay LgBiT Annexin V Healthy Cell SmBiT Phosphatidyl Serine Annexin V PS confined to inner leaflet Cell membrane intact Luminescence (RLU) negative Fluorescence (RFU) negative Necrosis Detection Reagent Early Apoptosis NanoLuc Luciferase PS translocation to outer leaflet Cell membrane intact Luminescence (RLU) POSITIVE Fluorescence (RFU) negative Secondary Necrosis RealTime-Glo Annexin V Assay Principle. The detection reagent contains two Annexin V fusion proteins containing complementary domains of NanoLuc luciferase (Annexin V-LgBiT and Annexin V-SmBiT). The Reagent also contains a cell impermeant, profluorescent DNA dye. Luminescence remains low until PS exposure brings the annexin fusion proteins close together, forming a functional luciferase. A fluorescent signal is generated upon loss of membrane integrity during late stage apoptosis. PS on inner and outer leaflet Cell membrane compromised Luminescence (RLU) POSITIVE Fluorescence (RFU) POSITIVE RealTime-Glo Annexin V Assay 원리 Detection 시약에는 NanoLuc luciferase 의 complementary domain 을포함한두개의 Annexin V fusion protein (Annexin V-LgBiT & Annexin V-SmBiT) 이포함되어있습니다. 또한세포막을투과하지못하는 pro-fluorescent DNA dye 도포함되어있습니다. 발광 (luminescence) 은 PS 와 Annexin V fusion 단백질이결합 (functional luciferase 형성 ) 할때까지낮게유지됩니다. 형광 (fluorescent signal) 은 late apoptosis 단계에서세포막손실에의해생성됩니다. * Assay 제품명앞의바색깔에따라흡광, 형광, 발광 assay 기법을확인하실수있습니다.
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